To screen new biological formulations, accurate and efficient application technology, field test was conducted with 72% Bacillus thuringiensis(Bt)·monosultap WP, 8% lambda-cyhalothrin·emamectin benzoate SA a...To screen new biological formulations, accurate and efficient application technology, field test was conducted with 72% Bacillus thuringiensis(Bt)·monosultap WP, 8% lambda-cyhalothrin·emamectin benzoate SA and 3.6% lambda-cyhalothrin·Bt SA through manual spraying and unmanned aerial vehicle spraying. Test results and comprehensive evaluation analysis demonstrated that 72% Bt·monosultap WP and 8%lambda-cyhalothrin·emamectin benzoate SA by manual spraying and unmanned aerial vehicle spraying had good prevention and control effect on strains and internodes damaged by Tryporyza intacta Snellen in middle and late stage, which were ideal new biological formulations with high effi-ciency and low risk for prevention and control of T. intacta in middle and late stage, and could be popularized in sugarcane area. 72% Bt·monosultap WP at the dose of 3 000 g/hectare and 8% lambda-cyhalothrin·emamectin benzoate SA at the dose of 750 mL/hectare could be sprayed in mid September at the peak occurrence period of the forth and fifth generations of T. intacta and Proceras venosatus. Agents were diluted with 900 kg water per hectare, and manually sprayed with electric knapsack sprayer; or agents were diluted with 15 kg of Haoyang aerial control special addi-tives and water per hectare, and sprayed with unmanned aerial vehicle. The control effect against borer-damaged strain rate was above 81.3% and that against borer-damaged internode rate was above 88.6%.展开更多
Total concentration of heavy metals(Cd, Cr, Cu, Fe, Pb, Ni, Mn and Zn) was estimated in the redeveloping soil of mine spoil under 5 yr old plantations of four woody species namely: Albizia lebbeck, Albizia procera, Te...Total concentration of heavy metals(Cd, Cr, Cu, Fe, Pb, Ni, Mn and Zn) was estimated in the redeveloping soil of mine spoil under 5 yr old plantations of four woody species namely: Albizia lebbeck, Albizia procera, Tectona grandis and Dendrocalamus strictus. The data recorded in the present study were compared with other unplanted coal mine spoil colliery, which was around to the study site and adjoining area of dry tropical forest. Among all the heavy metals, the maximum concentration was found for Fe and minimum for Cd. However, among all four species, total concentrations of these heavy metals were recorded maximally in the plantation plots of T. grandis except for Fe, while minimally in A. lebbeck except for Zn, whereas, the maximum concentration of Fe and Zn was in the plantation plots of D. strictus and A. procera. Statistical analysis revealed significant differences due to species for all the heavy metals except Cu. Among four species, A. lebbeck, A. procera and D. strictus showed more efficient for reducing heavy metal concentrations whereas T. grandis was not more effective to reduce heavy metal concentrations in redeveloping soil of mine spoil.展开更多
AIM： To evaluate the anticancer property of the dried latex （DL） of Calotropis procera, a tropical medicinal plant, in the X15-myc transgenic mouse model of hepatocellular carcinoma and to elucidate its mechanism o...AIM： To evaluate the anticancer property of the dried latex （DL） of Calotropis procera, a tropical medicinal plant, in the X15-myc transgenic mouse model of hepatocellular carcinoma and to elucidate its mechanism of action in cell culture. METHODS： The young transgenic mice were orally fed with the aqueous suspension of DL （400 mg/kg for 5 d/wk） for 15 wk and their liver was examined for histopathological changes at 20 wk. Serum levels of vascu- lar endothelial growth factor （VEGF） were also measured in these animals. To characterize the active fraction, DL was extracted with petroleum ether followed by methanol. The methanolic extract was sub-fractionated on a silica gel G column using a combination of non-polar and polar solvents and eleven fractions were obtained. Each fraction was analysed for cytotoxic effect on hepatoma （Huh7） and non-hepatoma （COS-1） cell lines and nontransformed hepatocytes （AML12） using tetrazolium （MTT） assay. Finally, the mechanism of cell death was investigated by measuring the levels of Bcl2, caspase 3 and DNA fragmentation. RESULTS： DL treatment of mice showed a complete protection against hepatocarcinogenesis. No adverse effect was observed in these animals. The serum VEGF level was significantly lowered in the treated mice as compared to control animals. Cell culture studies revealed that the methanolic extract of DL as well as its fraction 8 induced extensive cell death in both Huh-7 and COS-1 cells while AML12 cells were spared. This was accompanied by extensive fragmentation of DNA in Huh-7 and COS-1 cells. No change in the levels of canonical markers of apoptosis such as Bcl2 and caspase 3 was observed. CONCLUSION： DL of C. procera has the potential for anti-cancer therapy due to its differentJable targets and non-interference with regular pathway of apoptosis.展开更多
Medicinal plants have been used to treat various ailments of the poor population around the world;hence the interest among researchers to know the active ingredients of certain plants has being increased.The Calotropi...Medicinal plants have been used to treat various ailments of the poor population around the world;hence the interest among researchers to know the active ingredients of certain plants has being increased.The Calotropis procera(C.procera) is a plant original from Africa,commonly found in northeastern Brazil.It is well known for their pharmacological properties,since it produces large amounts of latex.The important role that medicinal plants play in folk medicine has led us to develop this article in order to review the major pharmacological activities of C. procera.展开更多
Objective:To ascertain analgesic,antibacterial and central nervous system(CNS)depressant activities of ethyl acetate,dichloromethane and carbon tetrachloride fractions of methanol extract of Albizia proceru(A.procera)...Objective:To ascertain analgesic,antibacterial and central nervous system(CNS)depressant activities of ethyl acetate,dichloromethane and carbon tetrachloride fractions of methanol extract of Albizia proceru(A.procera)leaves.Methods:Leaves extracts of A.procera were tested for analgesic activity by acetic acid induced and formalin test method in mice.The in vitro antibacterial activity was performed by agar well diffusion method.CNS depressant activity was evaluated by hole cross and open field tests.Results:All the extracts at 200 mg/kg exhibited significant(P<0.01)analgesic activity in acetic acid induced and formalin tests method in mice.Analgesic activity of the ethyl acetate fraction was almost same like as standard drug indomelhacin in acetic acid induced method.The CNS depressant activity of the extracts at 500 mg/kg was comparable to the positive control diazepam as determined by hole cross and open field test method.The extracts exhibited moderate antimicrobial activity against all the tested microorganisms(Staphylococcus aureus,Bacillus cereus,Pseudomonas aeruginosa,Esherichia coli,Shigetta soneii.Shigella boydii)at concentration of 0.8 mg/disc.The measured diameter of zone of inhibition for the extracts was within the range of 7 to 12 mm which was less than the standard kanamycin(16-24 mm).Conclusions:It is concluded that all the extracts possess potential analgesic and CNS depressants activity.This study also showed that different fractions of methanol extract could be potential sources of new antimicrobial agents.展开更多
Objective:To synthesize silver nanoparticles(AgNPs) by green methods using scrum latex of Calotropis procera at 80 ℃ and evaluate them against bacteria,dermatophytes and phytopathogenic fungi comparing with the activ...Objective:To synthesize silver nanoparticles(AgNPs) by green methods using scrum latex of Calotropis procera at 80 ℃ and evaluate them against bacteria,dermatophytes and phytopathogenic fungi comparing with the activity of untreated latex.Methods:The synthesis of AgNPs was performed by mixing 3%latex scrum extract with the same volume of silver nitrate(2 mmol/L) solution in round flask and heating in water bath at80 ℃.Characterization of silver particles were determined using UV-vis spectrophotometer,transmission electron microscopy(TEM),X-ray diffraction and Fourier transform infrared spectroscopy.The antimicrobial activity of the green synthesized AgNPs was determined against bacteria,dermatophytes and phytopathogenic fungi and compared to the crude untreated latex by agar-well diffusion methods.Results:Biosynthesis of latex silver nanoparticles was successfully obtained by green method.The formation of AgNPs has been confirmed by UV-vis,TEM microscopy.X-ray diffraction and Fourier transform infrared spectroscopy.TEM analysis showed that synthesized AgNPs are highly stable spherical shaped particles,well dispersed with a diameter ranged from 4 nm up to 25 nm and an average size of 12.33 nm.AgNPs showed strong antibacterial activity against Gram-negative bacteria(Escherichia coli,Pseudomonas aeruginosa and Serratia sp.) and antifungal activity against Trichophyton rubrum,Candida albicans and Aspergillus terreus.Conclusions:It can be concluded that serum latex of Calotropis pmcera was found to display strong potential for the synthesis of AgNPs as antimicrobial agents through rapid reduction of silver ions(Ag^+ to Ag^0).The green synthesized AgNPs were found to show higher antimicrobial efficacy than crude latex.展开更多
Calotropis procera(Aiton)W.T.Aiton,belonging to the family Apocynaceae,is C3 evergreen plant species in arid and semi-arid areas of the Punjab Province,Pakistan.It grows in a variety of habitats like salt affected and...Calotropis procera(Aiton)W.T.Aiton,belonging to the family Apocynaceae,is C3 evergreen plant species in arid and semi-arid areas of the Punjab Province,Pakistan.It grows in a variety of habitats like salt affected and waterlogged area,desert/semi-desert,roadside,wasteland,graveyard,forest,crop field,coastline,and river/canal bank.A total of 12 populations growing in different ecological regions were sampled to evaluate their growth,physio-biochemical,and anatomical responses to specific environmental condition.Population adapted to desert/semi-desert showed vigorous growth(plant height,shoot length,and number of leaves),enhanced photosynthetic level(chlorophyll a,chlorophyll b,carotenoids,and total chlorophyll),and apparent anatomical modifications such as increased stem radius,cuticle thickness,storage parenchyma tissues(cortex and pith),and vascular bundles in stems,while the maximum of midrib and lamina thickness,epidermal cells,cuticle thickness,cortical proportion,abaxial stomatal density,and its area in leaves.There was high plasticity in structural and functional features of these populations,which enable them to survive and tolerate under such hot and dry desert environment.Population of saline areas exhibited very critical modifications to sustain under salt prone environment.At physiological level,it possesses the maximum amount of organic osmolytes(glycine betaine and proline)and antioxidants(superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD)),while at anatomical level,it showed intensive sclerification,large phloem region(inner and outer),pith parenchyma cells,and metaxylem vessels in stems and leaves.The population of dry mountains showed very distinctive features,such as increased shoot ionic contents(K+and Ca2+),collenchyma and sclerenchyma thickness in stems,trichomes size,and numerous small stomata on abaxial surface of leaves.It is concluded that no definite or precise single character can be taken as a yardstick for adjudging the biomass production in this rubber bush weed population.展开更多
In Ethiopia,Cupressus lusitanica and Juniperus procera are important tree species.The incidence of the cypress aphid,Cinara cupressi,which has not been reported before on the exotic Cupressus lusitanica is becoming ca...In Ethiopia,Cupressus lusitanica and Juniperus procera are important tree species.The incidence of the cypress aphid,Cinara cupressi,which has not been reported before on the exotic Cupressus lusitanica is becoming catastrophic.The appearance of the insect was reported for the first time in 2003 in Ethiopia.However,information is scarce on the status of this species in northeast Amhara state.The objectives of this study were to assess the extent of damage,abundance and status of the cypress aphid on C.lusitanica and J.procera in the protected and cultivated forests of South Wollo,Ethiopia.Results reveal that tree compositions of the three study areas differed.The dominant tree species were C.lucitanica(15–80%),Olea europaea(5–90%)and J.procera(14–70%).The cypress aphid showed significantly higher levels of infestation on C.lusitanica(35–90%)than on J.procera(1–16%).Moreover,there was higher C.lusitanica mortality(40–93%).In contrast,there was low infestation(1–16%)on J.procera and no mortality.The results reveal that the cypress aphid causes enormous losses of C.lusitanica.Therefore,it is recommended that insect surveillance be strengthened and the introduction bioagents be considered and integrated with other insecticides to minimize the degree of C.lusitanica infestation and loss.展开更多
The African Rice Gall Midge, Orseolia oryzivora H. & G., is an important insect pest recorded in 20 countries in Sub-Sahara Africa. In Burkina Faso, the insect pest particularly prevails in Western and South-Weste...The African Rice Gall Midge, Orseolia oryzivora H. & G., is an important insect pest recorded in 20 countries in Sub-Sahara Africa. In Burkina Faso, the insect pest particularly prevails in Western and South-Western of the country where both biotic and abiotic conditions favor its development. The insect pest can damage up to 60% of rice tillers in Western Burkina Faso. A study was conducted during the 2011 wet season in the Kou Valley, located 25 km North-West of Bobo-Dioulas- so, Western Burkina Faso. Its objective was to evaluate the damage of this insect pest in farmers’ fields in relation with rice transplanting periods. The Kou Valley rice scheme, 1200 ha, was divided into two zones within which 48 farmers’ fields were randomly selected in relation with rice transplanting periods: P1: 1st period;P2: 2nd period and P3: 3rd period;that is 16 fields per period. Both agronomic and entomological evaluations were performed each week starting from the 21th day after transplanting (DAT) up to 84 DAT. Results showed that the highest average damage level (16% of galls) was recorded in the 3rd transplanting period. The highest larval and pupal parasitism was recorded in P3 at 84 DAT. Pupal parasitism due to Aprostocetus procerae Risbec was higher than larval parasitism due to Platygaster diplosisae Risbec. Lastly, the lowest yield (4.78 t/ha) was recorded in P3. These results can be used in the implementation of an integrated pest management strategy for this insect pest in the Kou Valley.展开更多
Species specific allometric equations are important for estimation and quantification of net volume and aboveground biomass of living trees. This study was basically focused on fitting total volume and aboveground bio...Species specific allometric equations are important for estimation and quantification of net volume and aboveground biomass of living trees. This study was basically focused on fitting total volume and aboveground biomass models for Juniperus procera plantations in Wondo Genet, Sidama Zone, Ethiopia. Data for fitting the total volume and aboveground biomass models were obtained by destructively sampling of trees from the ten diameter classes of the Juniperus procera plantation in the study area. A total of one hundred ten and fifty-one trees were destructively sampled to fit six total volume and six aboveground biomass models respectively. After important measurements of parameters have completed, model performance evaluation and selecting of best fit models were undertaken using standard error of estimates (SEE), coefficient of determination (R2), bias (B) and mean of the absolute value of errors (MAE). Accordingly, the total volume model Vt = -5.466 + 0.959Dbh0.005H003 and aboveground biomass model of B = 0.348Dbh0.57H0.032 were found to be the best predictive models for total tree volume and aboveground biomass respectively. In addition to the above results, diameter at breast height and total tree height data obtained from 69 circular sample plots of 0.01 ha area drawn from the plantation were used to estimate the total volume and aboveground biomass per hectare BEF which was estimated to be 0.64 Mg/m3. Generally, the selected models and computed BEF in this study are believed to be applied by different organisations and researches to estimate the total volume and aboveground biomass of the J. procera.展开更多
Background: Calotropis procera (C. procera), is an authentic plant naturally grown in the flora of Dead Sea region. Despite its toxicity, C. procera presents healing properties. However, it has not been implemented ye...Background: Calotropis procera (C. procera), is an authentic plant naturally grown in the flora of Dead Sea region. Despite its toxicity, C. procera presents healing properties. However, it has not been implemented yet in cosmetics as an active ingredient. Objective: The biological effects of C. procera callus extract on skin were elucidated solely and in combination with Dead Sea water (DSW). Methods: The capability of C. procera extract to protect against skin inflammation and irritation was tested on ex vivo human skin organ culture by LPS and SDS addition respectively. Viability and cytokine secretion were evaluated. The combination of C. procera extract with Dead Sea water was tested on full thickness skin equivalents. Gene expression and relevant biochemical markers for glycolysis, hypoxia and extracellular matrix balance were tested. Results: C. procera extract exhibits a protective biological activity against skin irritation and inflammation at the biochemical level. Furthermore, a combination of C. procera extract and DSW demonstrates a potential contribution for skin wellbeing via enhance energy production, resistance to hypoxia and extracellular matrix balance. Conclusions: Topical application of C. procera callus extract might support skin balance and wellbeing at the molecular level. Hence, it is recommended for new cosmetic formulae as standalone or in combination with Dead Sea water, in the effort to achieve anti-aging bio-activity that is working beyond skin aging symptoms, especially via skin calming effects and skin energy enhancement.展开更多
Objective:To examine thein vitroandin vivoanti-Trypanosoma evansi(T.evansi)activity ofsaponins-rich fraction ofCalotropis procera(cpsf)leaves as well as the effect of the fraction onthe parasite-induced anemia.Methods...Objective:To examine thein vitroandin vivoanti-Trypanosoma evansi(T.evansi)activity ofsaponins-rich fraction ofCalotropis procera(cpsf)leaves as well as the effect of the fraction onthe parasite-induced anemia.Methods:A 60-minutes time course experiment was conductedwith various concentrations of the fraction using a 96-well microtiter plate technique,andsubsequently used to treat experimentallyT.evansiinfected rats at 100 and 200 mg/kg bodyweight.Index of anemia was analyzed in all animals during the experiment.Results:The cpsfdid not demonstrate anin vitroantitrypanosomal activity.Further,the cpsf treatments did notsignificantly(P>0.05)keep the parasites lower than the infected untreated groups.At the end ofthe experiment,allT.evansiinfected rats developed anemia whose severity was not significantly(P>0.05)ameliorated by the cpsf treatment.Conclusions:It was concluded that saponins derivedfromCalotropis proceraleaves could not elicitin vitroandin vivoactivities againstT.evansi.展开更多
Objective:To evaluate the toxicological and psychotropic properties of Calotropis(C.)procera.Methods:C.procera leaves and root-bark aqueous extracts were evaluated for their toxic and behavioral effects using adult mi...Objective:To evaluate the toxicological and psychotropic properties of Calotropis(C.)procera.Methods:C.procera leaves and root-bark aqueous extracts were evaluated for their toxic and behavioral effects using adult mice.Toxicity studies were carried out using Organisation for Economic Cooperation and Development guidelines 423 and 407 for acute and subacute evaluation.Behavioral studies were performed using traction test,fireplace test,hole-board test and forced-swimming test to evaluate the sedative,anxiety and depressive-like activities of the extracts.Results:Very low acute toxicity was observed in mice that received both leaves and rootbark extracts.The subacute test showed some morphological,biochemical and hematological changes in the treated groups.Behavioral assessment demonstrated anxiety effects on mice for C.procera leaf extract(400 mg/kg of body weight).Conclusions:The acute use of C.procera(leaves and root-barks)aqueous extracts could be considered as low toxic.However,their repeated uses could have harmful effect on some organs.Likewise,a single dose up to 400 mg/kg body weight of these extracts produce no sedative or depressive-like effect,but they possess possible dose dependent anxiety effect.Yet,more studies are necessary to relate these results to the chemical profile of the plant extracts.展开更多
Objective: To examine three plant extracts [Lumnitzera racemosa(Combretaceae)(L.racemosa), Albizia procera(Fabaceae)(A.procera) and Cananga odorata(Annonaceae)] for their potential as source of photosensitizers in pho...Objective: To examine three plant extracts [Lumnitzera racemosa(Combretaceae)(L.racemosa), Albizia procera(Fabaceae)(A.procera) and Cananga odorata(Annonaceae)] for their potential as source of photosensitizers in photodynamic therapy.Methods: Human mammary adenocarcinoma(MCF-7) cells were treated with the plant extracts, which were irradiated with 5.53 m W and 0.553 mW broadband light.Cell viability was assessed using MTT assay and induction of apoptosis was determined using terminal deoxynucleotidyl transferase-dUTP nick end labeling assay.Results: The crude ethanolic extracts, independently, were nontoxic against cancer and non-cancer cells but when irradiated with 5.53 mW broadband light, L.racemosa and A.procera extracts were cytotoxic against MCF-7 with IC_(50) of 11.63 mg/mL and10.73 mg/mL, respectively.With 0.553 mW broadband light, the IC_(50) values were higher at 17.14 mg/mL and 19.59 mg/mL, respectively.Photoactivated L.racemosa and A.procera extracts were found to be more cytotoxic against MCF-7 than the non-cancer cell line, human dermal fibroblast-neonatal.Moreover, the cytotoxicity of the extracts was mediated by apoptosis.Conclusions: Two of the plant extracts used, L.racemosa and A.procera were toxic and induced apoptosis to mammary cell adenocarcinoma, MCF-7 when photoactivated.These extracts were also more toxic to human cancer than non-cancer cell lines.展开更多
Nontimber forest products are a source of income for women in rural African communities. However, these products are frequently damaged by insect pests. The present study investigates the diversity and damage rates of...Nontimber forest products are a source of income for women in rural African communities. However, these products are frequently damaged by insect pests. The present study investigates the diversity and damage rates of insect pests that attack Carapa procera seeds and Lophira lanceolata fruits. The experiment was set up in western Burkina Faso and, for C. carapa, consisted of pests collected from seeds that had fallen to the ground and from stockpiled seeds. For L. lanceolata, pests were collected from fruits on the trees, and on the ground. The collected samples were sent to the laboratory to estimate the proportion of damaged seeds/fruits and rear the insects. The results showed that Ephestia spp., Tribolium castaneum, Oryzeaphilus spp., and Tenebroides mauritanicus were the pests of Carapa procera seeds and Lophira lanceolata fruits. Ephestia spp. was recorded as the main pest of both C. proeera and L. lanceolata, whereas T. castaneum was only detected from seeds of L. lanceolata. For C. procera, the stocks were the most infested （29 %） by Ephestia spp. The infestation rate of fruits of L. lanceolata by Ephestia spp, on trees （31.42 ± 3.75 %） was less than the rate of fruits by T. castaneum on the ground （44.00 ± 3.5 %）. The different body sizes of Ephestia spp. may indicate the occurrence of two putative species, one from C. procera and another one from L. lanceolata. This work provides important information that could contribute to the setting up of a local-scale sustainable management framework for oil tree pests in Burkina Faso and surrounding countries.展开更多
An experiment was conducted to assess the effect of Phosphorous （P） fertilizer （i.e. TSP or triple super phosphate @ 80 kg/hm^2） on seedling growth and nodulation capabilities of three potentially important agrofo...An experiment was conducted to assess the effect of Phosphorous （P） fertilizer （i.e. TSP or triple super phosphate @ 80 kg/hm^2） on seedling growth and nodulation capabilities of three potentially important agroforestry tree species （Acacia auriculiformis, Albizia lebbeck and Albizia procera） of Bangladesh. The study was conducted in nursery beds with six-month-old polybag seedlings ofA. auriculiformis, A. lebbeck and A. procera. The effects of P fertilizer on seedling growth and nodulation were compared with that of the seedlings grown in control （i.e. unfertilized soil）. The observations revealed that the seedling growth was enhanced significantly with the application of P fertilizer. The growth was found more pronounced in A. auriculiformis, whereas it was not apparent and shows depressed growth in case ofA. lebbeck. The study also suggests that the nodulation in terms of nodule number and size was also increased significantly with the application of P fertilizer.展开更多
基金Supported by Special Fund of China Agricultural (Sugar) Industry Research System(CARS-170303)Training Project of Yunling Industry and Technology Leading Talents "Control of Sugarcane Harm-ful Organisms"(2018LJRC56)Special Fund of Agricultural Industry Research System in Yunnan Province
文摘To screen new biological formulations, accurate and efficient application technology, field test was conducted with 72% Bacillus thuringiensis(Bt)·monosultap WP, 8% lambda-cyhalothrin·emamectin benzoate SA and 3.6% lambda-cyhalothrin·Bt SA through manual spraying and unmanned aerial vehicle spraying. Test results and comprehensive evaluation analysis demonstrated that 72% Bt·monosultap WP and 8%lambda-cyhalothrin·emamectin benzoate SA by manual spraying and unmanned aerial vehicle spraying had good prevention and control effect on strains and internodes damaged by Tryporyza intacta Snellen in middle and late stage, which were ideal new biological formulations with high effi-ciency and low risk for prevention and control of T. intacta in middle and late stage, and could be popularized in sugarcane area. 72% Bt·monosultap WP at the dose of 3 000 g/hectare and 8% lambda-cyhalothrin·emamectin benzoate SA at the dose of 750 mL/hectare could be sprayed in mid September at the peak occurrence period of the forth and fifth generations of T. intacta and Proceras venosatus. Agents were diluted with 900 kg water per hectare, and manually sprayed with electric knapsack sprayer; or agents were diluted with 15 kg of Haoyang aerial control special addi-tives and water per hectare, and sprayed with unmanned aerial vehicle. The control effect against borer-damaged strain rate was above 81.3% and that against borer-damaged internode rate was above 88.6%.
文摘Total concentration of heavy metals(Cd, Cr, Cu, Fe, Pb, Ni, Mn and Zn) was estimated in the redeveloping soil of mine spoil under 5 yr old plantations of four woody species namely: Albizia lebbeck, Albizia procera, Tectona grandis and Dendrocalamus strictus. The data recorded in the present study were compared with other unplanted coal mine spoil colliery, which was around to the study site and adjoining area of dry tropical forest. Among all the heavy metals, the maximum concentration was found for Fe and minimum for Cd. However, among all four species, total concentrations of these heavy metals were recorded maximally in the plantation plots of T. grandis except for Fe, while minimally in A. lebbeck except for Zn, whereas, the maximum concentration of Fe and Zn was in the plantation plots of D. strictus and A. procera. Statistical analysis revealed significant differences due to species for all the heavy metals except Cu. Among four species, A. lebbeck, A. procera and D. strictus showed more efficient for reducing heavy metal concentrations whereas T. grandis was not more effective to reduce heavy metal concentrations in redeveloping soil of mine spoil.
基金Supported by the core grant of International Centre for Genetic Engineering and Biotechnology. New Delhi
文摘AIM： To evaluate the anticancer property of the dried latex （DL） of Calotropis procera, a tropical medicinal plant, in the X15-myc transgenic mouse model of hepatocellular carcinoma and to elucidate its mechanism of action in cell culture. METHODS： The young transgenic mice were orally fed with the aqueous suspension of DL （400 mg/kg for 5 d/wk） for 15 wk and their liver was examined for histopathological changes at 20 wk. Serum levels of vascu- lar endothelial growth factor （VEGF） were also measured in these animals. To characterize the active fraction, DL was extracted with petroleum ether followed by methanol. The methanolic extract was sub-fractionated on a silica gel G column using a combination of non-polar and polar solvents and eleven fractions were obtained. Each fraction was analysed for cytotoxic effect on hepatoma （Huh7） and non-hepatoma （COS-1） cell lines and nontransformed hepatocytes （AML12） using tetrazolium （MTT） assay. Finally, the mechanism of cell death was investigated by measuring the levels of Bcl2, caspase 3 and DNA fragmentation. RESULTS： DL treatment of mice showed a complete protection against hepatocarcinogenesis. No adverse effect was observed in these animals. The serum VEGF level was significantly lowered in the treated mice as compared to control animals. Cell culture studies revealed that the methanolic extract of DL as well as its fraction 8 induced extensive cell death in both Huh-7 and COS-1 cells while AML12 cells were spared. This was accompanied by extensive fragmentation of DNA in Huh-7 and COS-1 cells. No change in the levels of canonical markers of apoptosis such as Bcl2 and caspase 3 was observed. CONCLUSION： DL of C. procera has the potential for anti-cancer therapy due to its differentJable targets and non-interference with regular pathway of apoptosis.
文摘Medicinal plants have been used to treat various ailments of the poor population around the world;hence the interest among researchers to know the active ingredients of certain plants has being increased.The Calotropis procera(C.procera) is a plant original from Africa,commonly found in northeastern Brazil.It is well known for their pharmacological properties,since it produces large amounts of latex.The important role that medicinal plants play in folk medicine has led us to develop this article in order to review the major pharmacological activities of C. procera.
基金Supported by the National Science and Technology(NST),Government of the People's Republic of Bangladesh(Grant No.39.012.002.01.03.018.2012/256)
文摘Objective:To ascertain analgesic,antibacterial and central nervous system(CNS)depressant activities of ethyl acetate,dichloromethane and carbon tetrachloride fractions of methanol extract of Albizia proceru(A.procera)leaves.Methods:Leaves extracts of A.procera were tested for analgesic activity by acetic acid induced and formalin test method in mice.The in vitro antibacterial activity was performed by agar well diffusion method.CNS depressant activity was evaluated by hole cross and open field tests.Results:All the extracts at 200 mg/kg exhibited significant(P<0.01)analgesic activity in acetic acid induced and formalin tests method in mice.Analgesic activity of the ethyl acetate fraction was almost same like as standard drug indomelhacin in acetic acid induced method.The CNS depressant activity of the extracts at 500 mg/kg was comparable to the positive control diazepam as determined by hole cross and open field test method.The extracts exhibited moderate antimicrobial activity against all the tested microorganisms(Staphylococcus aureus,Bacillus cereus,Pseudomonas aeruginosa,Esherichia coli,Shigetta soneii.Shigella boydii)at concentration of 0.8 mg/disc.The measured diameter of zone of inhibition for the extracts was within the range of 7 to 12 mm which was less than the standard kanamycin(16-24 mm).Conclusions:It is concluded that all the extracts possess potential analgesic and CNS depressants activity.This study also showed that different fractions of methanol extract could be potential sources of new antimicrobial agents.
文摘Objective:To synthesize silver nanoparticles(AgNPs) by green methods using scrum latex of Calotropis procera at 80 ℃ and evaluate them against bacteria,dermatophytes and phytopathogenic fungi comparing with the activity of untreated latex.Methods:The synthesis of AgNPs was performed by mixing 3%latex scrum extract with the same volume of silver nitrate(2 mmol/L) solution in round flask and heating in water bath at80 ℃.Characterization of silver particles were determined using UV-vis spectrophotometer,transmission electron microscopy(TEM),X-ray diffraction and Fourier transform infrared spectroscopy.The antimicrobial activity of the green synthesized AgNPs was determined against bacteria,dermatophytes and phytopathogenic fungi and compared to the crude untreated latex by agar-well diffusion methods.Results:Biosynthesis of latex silver nanoparticles was successfully obtained by green method.The formation of AgNPs has been confirmed by UV-vis,TEM microscopy.X-ray diffraction and Fourier transform infrared spectroscopy.TEM analysis showed that synthesized AgNPs are highly stable spherical shaped particles,well dispersed with a diameter ranged from 4 nm up to 25 nm and an average size of 12.33 nm.AgNPs showed strong antibacterial activity against Gram-negative bacteria(Escherichia coli,Pseudomonas aeruginosa and Serratia sp.) and antifungal activity against Trichophyton rubrum,Candida albicans and Aspergillus terreus.Conclusions:It can be concluded that serum latex of Calotropis pmcera was found to display strong potential for the synthesis of AgNPs as antimicrobial agents through rapid reduction of silver ions(Ag^+ to Ag^0).The green synthesized AgNPs were found to show higher antimicrobial efficacy than crude latex.
文摘Calotropis procera(Aiton)W.T.Aiton,belonging to the family Apocynaceae,is C3 evergreen plant species in arid and semi-arid areas of the Punjab Province,Pakistan.It grows in a variety of habitats like salt affected and waterlogged area,desert/semi-desert,roadside,wasteland,graveyard,forest,crop field,coastline,and river/canal bank.A total of 12 populations growing in different ecological regions were sampled to evaluate their growth,physio-biochemical,and anatomical responses to specific environmental condition.Population adapted to desert/semi-desert showed vigorous growth(plant height,shoot length,and number of leaves),enhanced photosynthetic level(chlorophyll a,chlorophyll b,carotenoids,and total chlorophyll),and apparent anatomical modifications such as increased stem radius,cuticle thickness,storage parenchyma tissues(cortex and pith),and vascular bundles in stems,while the maximum of midrib and lamina thickness,epidermal cells,cuticle thickness,cortical proportion,abaxial stomatal density,and its area in leaves.There was high plasticity in structural and functional features of these populations,which enable them to survive and tolerate under such hot and dry desert environment.Population of saline areas exhibited very critical modifications to sustain under salt prone environment.At physiological level,it possesses the maximum amount of organic osmolytes(glycine betaine and proline)and antioxidants(superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD)),while at anatomical level,it showed intensive sclerification,large phloem region(inner and outer),pith parenchyma cells,and metaxylem vessels in stems and leaves.The population of dry mountains showed very distinctive features,such as increased shoot ionic contents(K+and Ca2+),collenchyma and sclerenchyma thickness in stems,trichomes size,and numerous small stomata on abaxial surface of leaves.It is concluded that no definite or precise single character can be taken as a yardstick for adjudging the biomass production in this rubber bush weed population.
基金financially supported by Wollo University,Ethiopia
文摘In Ethiopia,Cupressus lusitanica and Juniperus procera are important tree species.The incidence of the cypress aphid,Cinara cupressi,which has not been reported before on the exotic Cupressus lusitanica is becoming catastrophic.The appearance of the insect was reported for the first time in 2003 in Ethiopia.However,information is scarce on the status of this species in northeast Amhara state.The objectives of this study were to assess the extent of damage,abundance and status of the cypress aphid on C.lusitanica and J.procera in the protected and cultivated forests of South Wollo,Ethiopia.Results reveal that tree compositions of the three study areas differed.The dominant tree species were C.lucitanica(15–80%),Olea europaea(5–90%)and J.procera(14–70%).The cypress aphid showed significantly higher levels of infestation on C.lusitanica(35–90%)than on J.procera(1–16%).Moreover,there was higher C.lusitanica mortality(40–93%).In contrast,there was low infestation(1–16%)on J.procera and no mortality.The results reveal that the cypress aphid causes enormous losses of C.lusitanica.Therefore,it is recommended that insect surveillance be strengthened and the introduction bioagents be considered and integrated with other insecticides to minimize the degree of C.lusitanica infestation and loss.
文摘The African Rice Gall Midge, Orseolia oryzivora H. & G., is an important insect pest recorded in 20 countries in Sub-Sahara Africa. In Burkina Faso, the insect pest particularly prevails in Western and South-Western of the country where both biotic and abiotic conditions favor its development. The insect pest can damage up to 60% of rice tillers in Western Burkina Faso. A study was conducted during the 2011 wet season in the Kou Valley, located 25 km North-West of Bobo-Dioulas- so, Western Burkina Faso. Its objective was to evaluate the damage of this insect pest in farmers’ fields in relation with rice transplanting periods. The Kou Valley rice scheme, 1200 ha, was divided into two zones within which 48 farmers’ fields were randomly selected in relation with rice transplanting periods: P1: 1st period;P2: 2nd period and P3: 3rd period;that is 16 fields per period. Both agronomic and entomological evaluations were performed each week starting from the 21th day after transplanting (DAT) up to 84 DAT. Results showed that the highest average damage level (16% of galls) was recorded in the 3rd transplanting period. The highest larval and pupal parasitism was recorded in P3 at 84 DAT. Pupal parasitism due to Aprostocetus procerae Risbec was higher than larval parasitism due to Platygaster diplosisae Risbec. Lastly, the lowest yield (4.78 t/ha) was recorded in P3. These results can be used in the implementation of an integrated pest management strategy for this insect pest in the Kou Valley.
文摘Species specific allometric equations are important for estimation and quantification of net volume and aboveground biomass of living trees. This study was basically focused on fitting total volume and aboveground biomass models for Juniperus procera plantations in Wondo Genet, Sidama Zone, Ethiopia. Data for fitting the total volume and aboveground biomass models were obtained by destructively sampling of trees from the ten diameter classes of the Juniperus procera plantation in the study area. A total of one hundred ten and fifty-one trees were destructively sampled to fit six total volume and six aboveground biomass models respectively. After important measurements of parameters have completed, model performance evaluation and selecting of best fit models were undertaken using standard error of estimates (SEE), coefficient of determination (R2), bias (B) and mean of the absolute value of errors (MAE). Accordingly, the total volume model Vt = -5.466 + 0.959Dbh0.005H003 and aboveground biomass model of B = 0.348Dbh0.57H0.032 were found to be the best predictive models for total tree volume and aboveground biomass respectively. In addition to the above results, diameter at breast height and total tree height data obtained from 69 circular sample plots of 0.01 ha area drawn from the plantation were used to estimate the total volume and aboveground biomass per hectare BEF which was estimated to be 0.64 Mg/m3. Generally, the selected models and computed BEF in this study are believed to be applied by different organisations and researches to estimate the total volume and aboveground biomass of the J. procera.
文摘Background: Calotropis procera (C. procera), is an authentic plant naturally grown in the flora of Dead Sea region. Despite its toxicity, C. procera presents healing properties. However, it has not been implemented yet in cosmetics as an active ingredient. Objective: The biological effects of C. procera callus extract on skin were elucidated solely and in combination with Dead Sea water (DSW). Methods: The capability of C. procera extract to protect against skin inflammation and irritation was tested on ex vivo human skin organ culture by LPS and SDS addition respectively. Viability and cytokine secretion were evaluated. The combination of C. procera extract with Dead Sea water was tested on full thickness skin equivalents. Gene expression and relevant biochemical markers for glycolysis, hypoxia and extracellular matrix balance were tested. Results: C. procera extract exhibits a protective biological activity against skin irritation and inflammation at the biochemical level. Furthermore, a combination of C. procera extract and DSW demonstrates a potential contribution for skin wellbeing via enhance energy production, resistance to hypoxia and extracellular matrix balance. Conclusions: Topical application of C. procera callus extract might support skin balance and wellbeing at the molecular level. Hence, it is recommended for new cosmetic formulae as standalone or in combination with Dead Sea water, in the effort to achieve anti-aging bio-activity that is working beyond skin aging symptoms, especially via skin calming effects and skin energy enhancement.
基金This study was supported,in part,by the Education Trust FundABU desk office with reference ETF/DESS/AST&D/ABU ZARIA
文摘Objective:To examine thein vitroandin vivoanti-Trypanosoma evansi(T.evansi)activity ofsaponins-rich fraction ofCalotropis procera(cpsf)leaves as well as the effect of the fraction onthe parasite-induced anemia.Methods:A 60-minutes time course experiment was conductedwith various concentrations of the fraction using a 96-well microtiter plate technique,andsubsequently used to treat experimentallyT.evansiinfected rats at 100 and 200 mg/kg bodyweight.Index of anemia was analyzed in all animals during the experiment.Results:The cpsfdid not demonstrate anin vitroantitrypanosomal activity.Further,the cpsf treatments did notsignificantly(P>0.05)keep the parasites lower than the infected untreated groups.At the end ofthe experiment,allT.evansiinfected rats developed anemia whose severity was not significantly(P>0.05)ameliorated by the cpsf treatment.Conclusions:It was concluded that saponins derivedfromCalotropis proceraleaves could not elicitin vitroandin vivoactivities againstT.evansi.
文摘Objective:To evaluate the toxicological and psychotropic properties of Calotropis(C.)procera.Methods:C.procera leaves and root-bark aqueous extracts were evaluated for their toxic and behavioral effects using adult mice.Toxicity studies were carried out using Organisation for Economic Cooperation and Development guidelines 423 and 407 for acute and subacute evaluation.Behavioral studies were performed using traction test,fireplace test,hole-board test and forced-swimming test to evaluate the sedative,anxiety and depressive-like activities of the extracts.Results:Very low acute toxicity was observed in mice that received both leaves and rootbark extracts.The subacute test showed some morphological,biochemical and hematological changes in the treated groups.Behavioral assessment demonstrated anxiety effects on mice for C.procera leaf extract(400 mg/kg of body weight).Conclusions:The acute use of C.procera(leaves and root-barks)aqueous extracts could be considered as low toxic.However,their repeated uses could have harmful effect on some organs.Likewise,a single dose up to 400 mg/kg body weight of these extracts produce no sedative or depressive-like effect,but they possess possible dose dependent anxiety effect.Yet,more studies are necessary to relate these results to the chemical profile of the plant extracts.
基金Supported by Institute of Biology,University of the Philippines,Diliman through TA#9774-362-499-439
文摘Objective: To examine three plant extracts [Lumnitzera racemosa(Combretaceae)(L.racemosa), Albizia procera(Fabaceae)(A.procera) and Cananga odorata(Annonaceae)] for their potential as source of photosensitizers in photodynamic therapy.Methods: Human mammary adenocarcinoma(MCF-7) cells were treated with the plant extracts, which were irradiated with 5.53 m W and 0.553 mW broadband light.Cell viability was assessed using MTT assay and induction of apoptosis was determined using terminal deoxynucleotidyl transferase-dUTP nick end labeling assay.Results: The crude ethanolic extracts, independently, were nontoxic against cancer and non-cancer cells but when irradiated with 5.53 mW broadband light, L.racemosa and A.procera extracts were cytotoxic against MCF-7 with IC_(50) of 11.63 mg/mL and10.73 mg/mL, respectively.With 0.553 mW broadband light, the IC_(50) values were higher at 17.14 mg/mL and 19.59 mg/mL, respectively.Photoactivated L.racemosa and A.procera extracts were found to be more cytotoxic against MCF-7 than the non-cancer cell line, human dermal fibroblast-neonatal.Moreover, the cytotoxicity of the extracts was mediated by apoptosis.Conclusions: Two of the plant extracts used, L.racemosa and A.procera were toxic and induced apoptosis to mammary cell adenocarcinoma, MCF-7 when photoactivated.These extracts were also more toxic to human cancer than non-cancer cell lines.
基金supported by Danida(10-002AU)within the framework of the collaborative research project Quali Tree
文摘Nontimber forest products are a source of income for women in rural African communities. However, these products are frequently damaged by insect pests. The present study investigates the diversity and damage rates of insect pests that attack Carapa procera seeds and Lophira lanceolata fruits. The experiment was set up in western Burkina Faso and, for C. carapa, consisted of pests collected from seeds that had fallen to the ground and from stockpiled seeds. For L. lanceolata, pests were collected from fruits on the trees, and on the ground. The collected samples were sent to the laboratory to estimate the proportion of damaged seeds/fruits and rear the insects. The results showed that Ephestia spp., Tribolium castaneum, Oryzeaphilus spp., and Tenebroides mauritanicus were the pests of Carapa procera seeds and Lophira lanceolata fruits. Ephestia spp. was recorded as the main pest of both C. proeera and L. lanceolata, whereas T. castaneum was only detected from seeds of L. lanceolata. For C. procera, the stocks were the most infested （29 %） by Ephestia spp. The infestation rate of fruits of L. lanceolata by Ephestia spp, on trees （31.42 ± 3.75 %） was less than the rate of fruits by T. castaneum on the ground （44.00 ± 3.5 %）. The different body sizes of Ephestia spp. may indicate the occurrence of two putative species, one from C. procera and another one from L. lanceolata. This work provides important information that could contribute to the setting up of a local-scale sustainable management framework for oil tree pests in Burkina Faso and surrounding countries.
文摘An experiment was conducted to assess the effect of Phosphorous （P） fertilizer （i.e. TSP or triple super phosphate @ 80 kg/hm^2） on seedling growth and nodulation capabilities of three potentially important agroforestry tree species （Acacia auriculiformis, Albizia lebbeck and Albizia procera） of Bangladesh. The study was conducted in nursery beds with six-month-old polybag seedlings ofA. auriculiformis, A. lebbeck and A. procera. The effects of P fertilizer on seedling growth and nodulation were compared with that of the seedlings grown in control （i.e. unfertilized soil）. The observations revealed that the seedling growth was enhanced significantly with the application of P fertilizer. The growth was found more pronounced in A. auriculiformis, whereas it was not apparent and shows depressed growth in case ofA. lebbeck. The study also suggests that the nodulation in terms of nodule number and size was also increased significantly with the application of P fertilizer.
