Probiotic fermentation is attractive for beverage processing.However,its regulatory role in quality of coix seed-chrysanthemum beverage(CCB)rich in phenolics and polysaccharides was unknown.Results showed that co-ferm...Probiotic fermentation is attractive for beverage processing.However,its regulatory role in quality of coix seed-chrysanthemum beverage(CCB)rich in phenolics and polysaccharides was unknown.Results showed that co-fermentation with Bifidobacterium animalis subsp.lactis BLa80(BLa80),Lactobacillus delbrueckii subsp.bulgaria LB42(LB42),Streptococcus salivarius subsp.thermophilus ST81(ST81),and Lactobacillus plantarum LP90(LP90)for 12 h combined their respective advantages to prepare fermented CCB with viable counts of 8.78 lg CFU/mL,high phenolics bioaccessibility(>60%),good sensory quality,and strong antioxidant activity.LP90 greatly contributed to the aroma.BLa80 and LP90 hydrolyzed resistant starch/polymer dextrin.BLa80 showed the strongest deglycosidic ability,decreasing bitterness.The compound probiotic fermentation led to the biotransformation of endogenous polysaccharides/phenolics and introduction of exogenous viable probiotics resistant to gastrointestinal digestion,facilitating the proliferation of beneficial gut microbiota and accumulation of their metabolites.These findings provided a useful strategy for dual regulation of high quality of plant-based beverage.展开更多
Corn silk,an agricultural by-product rich in bioactive compounds,has long remained underutilized.In this study,we systematically examined how probiotic co-fermentation influences the bioactivity,flavor profile,and met...Corn silk,an agricultural by-product rich in bioactive compounds,has long remained underutilized.In this study,we systematically examined how probiotic co-fermentation influences the bioactivity,flavor profile,and metabolic landscape of corn silk.The results demonstrated that fermentation significantly enhanced the total phenolic content by 73.31%and flavonoid content by 80.20%,along with a substantial improvement in antioxidant capacity.Volatile compound analysis identified 36 key aroma-related substances.Notably,4-methylguaiacol and 4-ethylguaiacol contributed to both improved flavor and antioxidant function,highlighting a synergistic relationship between sensory quality and bioactivity during fermentation.Non-targeted metabolomics further revealed substantial changes in the metabolic profile post-fermentation,with 110 differential metabolites identified—75 upregulated and 35 downregulated.These metabolites were primarily enriched in the TCA cycle,phenylpropanoid biosynthesis,and amino acid metabolism pathways.Together,these findings uncover the molecular mechanisms by which probiotic fermentation remodels metabolic networks to enhance both the functional and sensory attributes of corn silk.This work offers a theoretical foundation and technical guidance for the high-value utilization of agricultural by-products and the development of functional foods.展开更多
Several studies have examined the metabolomic changes in brown probiotic fermented milk beverages(BPFBs)prepared via single-and mixed-strain fermentation during storage.In this study,the pH,titratable acidity,viable c...Several studies have examined the metabolomic changes in brown probiotic fermented milk beverages(BPFBs)prepared via single-and mixed-strain fermentation during storage.In this study,the pH,titratable acidity,viable cell counts,sensory characteristics,and metabolite profiles were of BPFBs prepared using Lacticaseibacillus paracasei PC-01 alone(S group)and those prepared using Lacticaseibacillus paracasei PC-01 mixed with Lacticaseibacillus rhamnosus Probio-M9(C group)were compared.Notably,no significant differences in pH and titratable acidity were observed between the two groups(P>0.05).However,viable cell counts were significantly higher in the S group at D1 and D7(P<0.05).Notably,Probio-M9 addition did not only improve the sensory scores of BPFBs but also increased the content of short-chain polypeptides,amino acids,and amino acid derivatives after storage,enhancing the quality of BPFBs.Together,these findings offer a theoretical basis for improving the probiotic efficacy of BPFBs.展开更多
Ulcerative colitis(UC)treatment depends on the balance of intestinal macrophages polarization and gut homeostasis.Plant-derived active peptides have emerged as a valuable resource for novel therapeutic agents of UC.In...Ulcerative colitis(UC)treatment depends on the balance of intestinal macrophages polarization and gut homeostasis.Plant-derived active peptides have emerged as a valuable resource for novel therapeutic agents of UC.In present study,Ziziphi Spinosae Semen peptides obtained by Lactobacillus reuteri SY523(L.reuteri SY523)fermentation,FZSSP-L,significantly reduced the M1/M2 macrophage ratio,as evidenced by the decrease of M1 markers(CD86,iNOS)and the increase of M2 markers(CD206,Arg1),with a shifted cytokine profile characterized by the reduced TNF-α,IL-1β,IL-6 and elevated IL-10,suggesting its underlying anti-UC efficacy in vitro and in vivo.Meanwhile,FZSSP-L significantly remodeled the diversity and structure of the gut microbiota in UC mice,characterized by the enhanced abundances of probiotic bacteria,such as the Bacteroides sp.and Muribaculum intestinale,which was markedly negatively correlated with inflammatory-related indicators.Together,FZSSP-L from Ziziphi Spinosae Semen can alleviate UC through regulating the macrophages polarization and gut microbiota homeostasis,supporting its potential development as a nutraceutical for intestinal health.展开更多
Colorectal cancer(CRC),a leading cause of cancer-related morbidity and mortality globally,is strongly associated with gut dysbiosis and intestinal barrier dysfunction,which drive chronic inflammation and carcinogenesi...Colorectal cancer(CRC),a leading cause of cancer-related morbidity and mortality globally,is strongly associated with gut dysbiosis and intestinal barrier dysfunction,which drive chronic inflammation and carcinogenesis.Given the emerging role of fermented foods with probiotics in microbiota modulation,this study systematically evaluated the preventive effects of Lactiplantibacillus plantarum NCU0011129-fermented guava(FG)and sterilized FG(SFG)against azoxymethane/dextran sodium sulfate(AOM/DSS)-induced CRC in mice.Through histopathological assessments,gut microbiome analysis,and intestinal barrier function evaluation,FG and SFG significantly improved survival rates(p<0.05),reduced tumor burden,and mitigated colon shortening compared to unfermented guava(UFG).Notably,gut microbiota analysis revealed FG reconfigured the gut microbiota and enriched beneficial taxa(Lachnospiraceae,Oscillospirales,Bacteroidaceae,and Akkermansiaceae)negatively correlated with mortality and pro-inflammatory cytokines while suppressing CRC-linked genera(Erysipelotrichaceae,Desulfovibrionaceae,and Peptostreptococcaceae).Concurrently,FG and SFG restored colonic mucosal integrity via tight junction protein upregulation and enhanced short-chain fatty acid(SCFA)production.These results highlight FG and SFG as promising dietary interventions for CRC prevention,mediated through modulation of the microbiota-immune-barrier axis.展开更多
One factor that shapes the establishment of early neonatal intestinal microbiota is environmental microbial exposure,and probiotic application has been shown to promote health and growth of piglets.Thus,this study hyp...One factor that shapes the establishment of early neonatal intestinal microbiota is environmental microbial exposure,and probiotic application has been shown to promote health and growth of piglets.Thus,this study hypothesized that environmental probiotic application in early days of life would be beneficial to newborn piglets.This study aimed to investigate the effect of spraying a compound probiotic fermented liquid(CPFL)into the living environment of piglets on their early growth performance and immunity.This work included 68 piglets,which were randomized into probiotic and control groups.Blood and fecal samples were collected at 0,3,7,14,and 21 days of age.Spraying CPFL significantly reshaped the microbiota composition of the delivery room environment,increased piglets’daily weight gain and weaning weight(P<0.001),and modulated piglets’serum cytokine levels(increases in Ig A,Ig G,and IL-10;decrease in IFN-γ;P<0.05 in each case)in piglets.Additionally,spraying CPFL during early days of life modified piglets’gut microbiota structure and diversity,increased the abundance of some potentially beneficial bacteria(such as Bacteroides uniformis,Butyricimonas virosa,Parabacteroides distasonis,and Phascolarctobacterium succinatutens)and decreased the abundance of Escherichia coli(P<0.05).Interestingly,CPFL application also significantly enhanced the gut microbial bioactive potential and levels of several serum metabolites involved in the metabolism of vitamins(B2,B3,B6,and E),medium/long-chain fatty acids(caproic,tetradecanoic,and peptadecanoic acids),and dicarboxylic acids(azelaic and sebacic acids).Our study demonstrated that spraying CPFL significantly could improve piglets’growth performance and immunity,and the beneficial effects are associated with changes in the gut microbiota and host metabolism.Our study has provided novel data for future development of probiotic-based health-promoting strategies and expanded our knowledge of probiotic application in animal husbandry.展开更多
Blueberry juice(BJ)contains a high concentration of biological components and current study assessed potential alternatives regarding bioactive compounds in blueberry juice after fermentation with probiotics.After fer...Blueberry juice(BJ)contains a high concentration of biological components and current study assessed potential alternatives regarding bioactive compounds in blueberry juice after fermentation with probiotics.After fermentation with Lactobacillus plantarum and Lactobacillus casei,it was shown that BJ was characterized by more active substances,and the antioxidant capacity of BJ was also increased via the verification of the DPPH and the hydroxyl radical scavenging assays.Additionally,15 anthocyanins and 9 polyphenols were identified with HPLC-MS in the probiotic fermented blueberry juice(FBJ):the relative content of several anthocyanins and polyphenols increased after fermentation.The pretreatment of both BJ and FBJ could attenuate gastric injuries,suppress oxidative stress and inflammatory via the inhibition of NF-κB pathway and induction of PGE2 level.Moreover,the protective effects of FBJ were significantly stronger than those of BJ.The findings outlined in the current study demonstrated that BJ can be utilized as a protective strategy against alcohol-induced gastric injuries,and the effects can be strengthened by fermentation with probiotics.展开更多
In the present study freeze-and spray-dried powders from non-fermented and fermented beetroot juice with the addition of prebiotic carriers(inulin,oligofructose,Nutriose®)and maltodextrin were obtained and analyz...In the present study freeze-and spray-dried powders from non-fermented and fermented beetroot juice with the addition of prebiotic carriers(inulin,oligofructose,Nutriose®)and maltodextrin were obtained and analyzed for physicochemical and biological properties.Fermentation and carrier type affected the powders’quality to a greater extent than drying techniques.Higher betalains content was noted for non-fermented juice powders,while ferulic and syringic acids’derivatives for their fermented-juice-based counterparts.Oligofructose induced hydroxymethyl-L-furfural formation,but also together with inulin resulted in products with the strongest antioxidant capacity.Nutriose®had the greatest in vitro antiproliferative activity towards human leukemia cell lines,unlike oligofructose which was shown to stimulate their growth.Overall,fermentation that led to beetroot matrix modification and carrier type affected powders’quality toward their improved potential functional properties.展开更多
基金financial support from Guangdong Natural Science Foundation(2022B1515120083).
文摘Probiotic fermentation is attractive for beverage processing.However,its regulatory role in quality of coix seed-chrysanthemum beverage(CCB)rich in phenolics and polysaccharides was unknown.Results showed that co-fermentation with Bifidobacterium animalis subsp.lactis BLa80(BLa80),Lactobacillus delbrueckii subsp.bulgaria LB42(LB42),Streptococcus salivarius subsp.thermophilus ST81(ST81),and Lactobacillus plantarum LP90(LP90)for 12 h combined their respective advantages to prepare fermented CCB with viable counts of 8.78 lg CFU/mL,high phenolics bioaccessibility(>60%),good sensory quality,and strong antioxidant activity.LP90 greatly contributed to the aroma.BLa80 and LP90 hydrolyzed resistant starch/polymer dextrin.BLa80 showed the strongest deglycosidic ability,decreasing bitterness.The compound probiotic fermentation led to the biotransformation of endogenous polysaccharides/phenolics and introduction of exogenous viable probiotics resistant to gastrointestinal digestion,facilitating the proliferation of beneficial gut microbiota and accumulation of their metabolites.These findings provided a useful strategy for dual regulation of high quality of plant-based beverage.
基金supported by the Anhui Provincial Science and Technology Innovation Tackling Project[grant number 202423m10050005]the Key Research and Development Project of Anhui Province[grant number 2023n06020049]+1 种基金the Natural Science Foundation of Anhui Province[grant number 2308085MC112]the Major Science and Technology Project of Suzhou[grant number SZKJXM202315].
文摘Corn silk,an agricultural by-product rich in bioactive compounds,has long remained underutilized.In this study,we systematically examined how probiotic co-fermentation influences the bioactivity,flavor profile,and metabolic landscape of corn silk.The results demonstrated that fermentation significantly enhanced the total phenolic content by 73.31%and flavonoid content by 80.20%,along with a substantial improvement in antioxidant capacity.Volatile compound analysis identified 36 key aroma-related substances.Notably,4-methylguaiacol and 4-ethylguaiacol contributed to both improved flavor and antioxidant function,highlighting a synergistic relationship between sensory quality and bioactivity during fermentation.Non-targeted metabolomics further revealed substantial changes in the metabolic profile post-fermentation,with 110 differential metabolites identified—75 upregulated and 35 downregulated.These metabolites were primarily enriched in the TCA cycle,phenylpropanoid biosynthesis,and amino acid metabolism pathways.Together,these findings uncover the molecular mechanisms by which probiotic fermentation remodels metabolic networks to enhance both the functional and sensory attributes of corn silk.This work offers a theoretical foundation and technical guidance for the high-value utilization of agricultural by-products and the development of functional foods.
基金funded by the National Natural Science Foundation of China(Grant No.32261143729)Natural Science Foundation of Inner Mongolia(Grant No.2021BS03043)+1 种基金Fundamental Research Funds of Inner Mongolia Agricultural University(Grant No.BR22-11-05)Scientific Research Start-up Fund for High-level Talents of Inner Mongolia Agricultural University(NDYB2021-16).
文摘Several studies have examined the metabolomic changes in brown probiotic fermented milk beverages(BPFBs)prepared via single-and mixed-strain fermentation during storage.In this study,the pH,titratable acidity,viable cell counts,sensory characteristics,and metabolite profiles were of BPFBs prepared using Lacticaseibacillus paracasei PC-01 alone(S group)and those prepared using Lacticaseibacillus paracasei PC-01 mixed with Lacticaseibacillus rhamnosus Probio-M9(C group)were compared.Notably,no significant differences in pH and titratable acidity were observed between the two groups(P>0.05).However,viable cell counts were significantly higher in the S group at D1 and D7(P<0.05).Notably,Probio-M9 addition did not only improve the sensory scores of BPFBs but also increased the content of short-chain polypeptides,amino acids,and amino acid derivatives after storage,enhancing the quality of BPFBs.Together,these findings offer a theoretical basis for improving the probiotic efficacy of BPFBs.
基金supported by the Key Research and Development(R&D)Program of Shanxi Province(No.2022ZDYF121)the Shanxi Province Science Foundation(No.202203021221031)the Shanxi Province Science Foundation for Youths(No.202203021222007).
文摘Ulcerative colitis(UC)treatment depends on the balance of intestinal macrophages polarization and gut homeostasis.Plant-derived active peptides have emerged as a valuable resource for novel therapeutic agents of UC.In present study,Ziziphi Spinosae Semen peptides obtained by Lactobacillus reuteri SY523(L.reuteri SY523)fermentation,FZSSP-L,significantly reduced the M1/M2 macrophage ratio,as evidenced by the decrease of M1 markers(CD86,iNOS)and the increase of M2 markers(CD206,Arg1),with a shifted cytokine profile characterized by the reduced TNF-α,IL-1β,IL-6 and elevated IL-10,suggesting its underlying anti-UC efficacy in vitro and in vivo.Meanwhile,FZSSP-L significantly remodeled the diversity and structure of the gut microbiota in UC mice,characterized by the enhanced abundances of probiotic bacteria,such as the Bacteroides sp.and Muribaculum intestinale,which was markedly negatively correlated with inflammatory-related indicators.Together,FZSSP-L from Ziziphi Spinosae Semen can alleviate UC through regulating the macrophages polarization and gut microbiota homeostasis,supporting its potential development as a nutraceutical for intestinal health.
基金supported by the National Key Laboratory Project(Project No.20232BCD44005)Jiangxi Medicine Academy of Nutrition and Health Management 2022 Cultivation project(Project No.2022-PYXM-04)Jiangxi Provincial Agricultural Key Core Technology Tackling Issues(Project No.JXNK202303-05).
文摘Colorectal cancer(CRC),a leading cause of cancer-related morbidity and mortality globally,is strongly associated with gut dysbiosis and intestinal barrier dysfunction,which drive chronic inflammation and carcinogenesis.Given the emerging role of fermented foods with probiotics in microbiota modulation,this study systematically evaluated the preventive effects of Lactiplantibacillus plantarum NCU0011129-fermented guava(FG)and sterilized FG(SFG)against azoxymethane/dextran sodium sulfate(AOM/DSS)-induced CRC in mice.Through histopathological assessments,gut microbiome analysis,and intestinal barrier function evaluation,FG and SFG significantly improved survival rates(p<0.05),reduced tumor burden,and mitigated colon shortening compared to unfermented guava(UFG).Notably,gut microbiota analysis revealed FG reconfigured the gut microbiota and enriched beneficial taxa(Lachnospiraceae,Oscillospirales,Bacteroidaceae,and Akkermansiaceae)negatively correlated with mortality and pro-inflammatory cytokines while suppressing CRC-linked genera(Erysipelotrichaceae,Desulfovibrionaceae,and Peptostreptococcaceae).Concurrently,FG and SFG restored colonic mucosal integrity via tight junction protein upregulation and enhanced short-chain fatty acid(SCFA)production.These results highlight FG and SFG as promising dietary interventions for CRC prevention,mediated through modulation of the microbiota-immune-barrier axis.
基金supported by the earmarked fund for CARS-36the Science and Technology Major Projects of Inner Mongolia Autonomous Region(2021ZD0014)。
文摘One factor that shapes the establishment of early neonatal intestinal microbiota is environmental microbial exposure,and probiotic application has been shown to promote health and growth of piglets.Thus,this study hypothesized that environmental probiotic application in early days of life would be beneficial to newborn piglets.This study aimed to investigate the effect of spraying a compound probiotic fermented liquid(CPFL)into the living environment of piglets on their early growth performance and immunity.This work included 68 piglets,which were randomized into probiotic and control groups.Blood and fecal samples were collected at 0,3,7,14,and 21 days of age.Spraying CPFL significantly reshaped the microbiota composition of the delivery room environment,increased piglets’daily weight gain and weaning weight(P<0.001),and modulated piglets’serum cytokine levels(increases in Ig A,Ig G,and IL-10;decrease in IFN-γ;P<0.05 in each case)in piglets.Additionally,spraying CPFL during early days of life modified piglets’gut microbiota structure and diversity,increased the abundance of some potentially beneficial bacteria(such as Bacteroides uniformis,Butyricimonas virosa,Parabacteroides distasonis,and Phascolarctobacterium succinatutens)and decreased the abundance of Escherichia coli(P<0.05).Interestingly,CPFL application also significantly enhanced the gut microbial bioactive potential and levels of several serum metabolites involved in the metabolism of vitamins(B2,B3,B6,and E),medium/long-chain fatty acids(caproic,tetradecanoic,and peptadecanoic acids),and dicarboxylic acids(azelaic and sebacic acids).Our study demonstrated that spraying CPFL significantly could improve piglets’growth performance and immunity,and the beneficial effects are associated with changes in the gut microbiota and host metabolism.Our study has provided novel data for future development of probiotic-based health-promoting strategies and expanded our knowledge of probiotic application in animal husbandry.
基金supported by the Science Fund for Distinguished Young Scholars of Liaoning Province(2023010)China Agriculture Research System of MOF and MARA(CARS-29)+1 种基金the First Batch of Liaoning“Unveiling Leader”Scientific and Technological Projects(2021JH1/10400036)the General Project of Science and Technology Department of Liaoning Province(No.2022-MS-256).
文摘Blueberry juice(BJ)contains a high concentration of biological components and current study assessed potential alternatives regarding bioactive compounds in blueberry juice after fermentation with probiotics.After fermentation with Lactobacillus plantarum and Lactobacillus casei,it was shown that BJ was characterized by more active substances,and the antioxidant capacity of BJ was also increased via the verification of the DPPH and the hydroxyl radical scavenging assays.Additionally,15 anthocyanins and 9 polyphenols were identified with HPLC-MS in the probiotic fermented blueberry juice(FBJ):the relative content of several anthocyanins and polyphenols increased after fermentation.The pretreatment of both BJ and FBJ could attenuate gastric injuries,suppress oxidative stress and inflammatory via the inhibition of NF-κB pathway and induction of PGE2 level.Moreover,the protective effects of FBJ were significantly stronger than those of BJ.The findings outlined in the current study demonstrated that BJ can be utilized as a protective strategy against alcohol-induced gastric injuries,and the effects can be strengthened by fermentation with probiotics.
基金supported by“UPWR 2.0:international and interdisciplinary programme of development of Wrocław University of Environmental and Life Sciences”co-financed by the European Social Fund under the Operational Program Knowledge Education Development,under contract No.POWR.03.05.00-00-Z062/18 of June 4,2019.
文摘In the present study freeze-and spray-dried powders from non-fermented and fermented beetroot juice with the addition of prebiotic carriers(inulin,oligofructose,Nutriose®)and maltodextrin were obtained and analyzed for physicochemical and biological properties.Fermentation and carrier type affected the powders’quality to a greater extent than drying techniques.Higher betalains content was noted for non-fermented juice powders,while ferulic and syringic acids’derivatives for their fermented-juice-based counterparts.Oligofructose induced hydroxymethyl-L-furfural formation,but also together with inulin resulted in products with the strongest antioxidant capacity.Nutriose®had the greatest in vitro antiproliferative activity towards human leukemia cell lines,unlike oligofructose which was shown to stimulate their growth.Overall,fermentation that led to beetroot matrix modification and carrier type affected powders’quality toward their improved potential functional properties.
