Searching alternative feed antibiotics is always a study hotspot in the field of animal production. In this study, the antipathogenic activities and probiotics stimulatory effects of 30 kinds of herbs were screened th...Searching alternative feed antibiotics is always a study hotspot in the field of animal production. In this study, the antipathogenic activities and probiotics stimulatory effects of 30 kinds of herbs were screened through Oxford cup method and Hungate roll method. 15 herbs showed significantly antibacterial activities（P〈0.05） against Escherichia coli, Salmonella enteritidis, Salmonella typhimurium and Staphylococcus aureus. Seven herbs showed greatly stimulatory promoting effects on Lactobacillus acidophilus and Bifidobacterium longum. Finally, five herbs were combined for the developed formulation with selective antibacterial properties and they were Mume Fructus（35%）, Isatidis Folium（25%）, Moslae Herba（20%）, Chrysanthemi Indici Flos（13%） and Bupleuri Radix（7%）. The herbal formulation showed significantly antibacterial abilities against four pathogens and stimulatory promoting abilities on two probiotics in vitro and the equivalent activities in broiler chickens in vivo against E. coli and L. acidophilus. The toxicity study showed it had no toxicity, which indicated that it would be a kind of preferred candidate for an alternative antibiotic in future animal production.展开更多
Bifidobacterium animalis subsp.lactis 420(B420),serving as a probiotic helping in metabolic health,has been extensively studied.Since the lipids produced by Bifidobacterium animalis subsp.lactis 420(B420)exhibit varia...Bifidobacterium animalis subsp.lactis 420(B420),serving as a probiotic helping in metabolic health,has been extensively studied.Since the lipids produced by Bifidobacterium animalis subsp.lactis 420(B420)exhibit variable bioactivities,understanding lipid profile alterations across different cultivation phases could offer valuable insights for developing targeted production strategies that enhance both the yield and bioactivity of specific lipid constituents.However,studies elucidating these lipid profile changes throughout various cultivation phases are limited,yet such research is essential for facilitating effective production strategies aimed at optimizing the yield and bioactivity of desired lipid components.Linoleic acid supplementation has been reported to enhance the production of unsaturated fatty acids in Bifidobacterium animalis subsp.lactis 420(B420).This study aimed to reveal the lipidomics of B420 and its profile change during the cultivation.Using ultra-performance liquid chromatography/nano-electrospray ionization-tandem mass spectrometry,the investigation revealed a significantly diverse composition and relative intensity of lipids across different cultivation stages.A total of 862 lipids,categorized into 23 distinct lipid classes,were identified in B420.Among these,683 unsaturated lipids comprised nearly 70%of the total lipid pool.Following linoleic acid supplementation,the total number of identified lipid compounds increased to 891,with unsaturated lipids rising to 701,which included 509 polyunsaturated lipids.Additionally,the relative content of unsaturated lipids at each growth phase demonstrated a significant increase,peaking at 75.69%during the late stationary phase,which is 8.89%higher than observed in the absence of linoleic acid supplementation.The intensity of unsaturated fatty acyls increased to 13.55 times.Notably,linoleic acid induced the production of unsaturated lipids characterized by varying numbers and distributions of double bonds.For the first time,this study enhances our understanding of the lipid profile changes in B420 and reveals the compositional and quantitative variations of unsaturated lipids resulting from linoleic acid supplementation during cultivation.Our research provided a new understanding of the probiotic effects of B420 from a lipidomic perspective and elucidate the mechanisms underlying linoleic acid supplementation during cultivation.展开更多
基金supported by Hebei Science and Technology Support Program of China (13226602D)
文摘Searching alternative feed antibiotics is always a study hotspot in the field of animal production. In this study, the antipathogenic activities and probiotics stimulatory effects of 30 kinds of herbs were screened through Oxford cup method and Hungate roll method. 15 herbs showed significantly antibacterial activities（P〈0.05） against Escherichia coli, Salmonella enteritidis, Salmonella typhimurium and Staphylococcus aureus. Seven herbs showed greatly stimulatory promoting effects on Lactobacillus acidophilus and Bifidobacterium longum. Finally, five herbs were combined for the developed formulation with selective antibacterial properties and they were Mume Fructus（35%）, Isatidis Folium（25%）, Moslae Herba（20%）, Chrysanthemi Indici Flos（13%） and Bupleuri Radix（7%）. The herbal formulation showed significantly antibacterial abilities against four pathogens and stimulatory promoting abilities on two probiotics in vitro and the equivalent activities in broiler chickens in vivo against E. coli and L. acidophilus. The toxicity study showed it had no toxicity, which indicated that it would be a kind of preferred candidate for an alternative antibiotic in future animal production.
基金supported by the National Key Research and Development Program of China(2023YFE0104400).
文摘Bifidobacterium animalis subsp.lactis 420(B420),serving as a probiotic helping in metabolic health,has been extensively studied.Since the lipids produced by Bifidobacterium animalis subsp.lactis 420(B420)exhibit variable bioactivities,understanding lipid profile alterations across different cultivation phases could offer valuable insights for developing targeted production strategies that enhance both the yield and bioactivity of specific lipid constituents.However,studies elucidating these lipid profile changes throughout various cultivation phases are limited,yet such research is essential for facilitating effective production strategies aimed at optimizing the yield and bioactivity of desired lipid components.Linoleic acid supplementation has been reported to enhance the production of unsaturated fatty acids in Bifidobacterium animalis subsp.lactis 420(B420).This study aimed to reveal the lipidomics of B420 and its profile change during the cultivation.Using ultra-performance liquid chromatography/nano-electrospray ionization-tandem mass spectrometry,the investigation revealed a significantly diverse composition and relative intensity of lipids across different cultivation stages.A total of 862 lipids,categorized into 23 distinct lipid classes,were identified in B420.Among these,683 unsaturated lipids comprised nearly 70%of the total lipid pool.Following linoleic acid supplementation,the total number of identified lipid compounds increased to 891,with unsaturated lipids rising to 701,which included 509 polyunsaturated lipids.Additionally,the relative content of unsaturated lipids at each growth phase demonstrated a significant increase,peaking at 75.69%during the late stationary phase,which is 8.89%higher than observed in the absence of linoleic acid supplementation.The intensity of unsaturated fatty acyls increased to 13.55 times.Notably,linoleic acid induced the production of unsaturated lipids characterized by varying numbers and distributions of double bonds.For the first time,this study enhances our understanding of the lipid profile changes in B420 and reveals the compositional and quantitative variations of unsaturated lipids resulting from linoleic acid supplementation during cultivation.Our research provided a new understanding of the probiotic effects of B420 from a lipidomic perspective and elucidate the mechanisms underlying linoleic acid supplementation during cultivation.
