Probe molecule Eu(DBM)3 phen is made up of europium oxide(EuO),dibenzoylmethane(DBM) and1,10-phenanthroline(phen). The temperature sensitive paint(TSP) was compounded by the polymerization of the probe molecule, methy...Probe molecule Eu(DBM)3 phen is made up of europium oxide(EuO),dibenzoylmethane(DBM) and1,10-phenanthroline(phen). The temperature sensitive paint(TSP) was compounded by the polymerization of the probe molecule, methyl methacrylate(MMA) and the initiator of benzoyl peroxide(BPO).The structure, morphology, luminescence property of probe molecule and the temperature quenching property of the temperature sensitive paint(TSP) were characterized by infrared spectrometer, UV-vis spectrometer, scanning electron microscopy and fluorescence spectrometer respectively. The infrared spectrum and UV-vis spectra show that Eu and DBM form six membered rings, and Eu-O coordinate bonds form. The nanocrystals are in sphere-like morphology with an average size of approximately100 nm. Fluorescence spectra present that the performance of temperature quenching is excellent,what’s more, TSP sample has different temperature sensitivity in various temperature scope. Particularly,under excitation of 286 nm, TSP has a highest temperature sensitivity between 50 and 60 ℃, and the strongest fluorescence emission reaches a peak(615 nm). It indicated that probe molecule(Eu(DBM)phen) has strong luminescent intensity and the temperature quenching properties of Eu(DBM)phen/PMMA is good.展开更多
Nitroreductase（NTR） is a member of flavin-containing enzymes that exists widely in bacteria. Hypoxia,which is a characteristic of locally advanced solid tumors, resulting from an imbalance between oxygen consumption...Nitroreductase（NTR） is a member of flavin-containing enzymes that exists widely in bacteria. Hypoxia,which is a characteristic of locally advanced solid tumors, resulting from an imbalance between oxygen consumption and supply, can result in NTR overexpression. Using either nicotinamide adenine dinucleotide（NADH） or nicotinamide adenine dinucleotide phosphate（NADPH） as a source of reducing equivalents, NTR can catalyze the reduction of nitroaromatic compounds to the corresponding amines.Based on this reduction mechanism, NTR can be applied not only in the bioremediation and degradation of organic nitrogen compounds, but also in the development of NTR-targeted fluorescent probes to detect the hypoxic status of cancer cells. This review aims to provide a summary of the progress in fluorescent probes for NTR in recent years and elucidate the main fluorescent mechanisms that have been applied to design probes.展开更多
Cu(II)ions play a critical role in tumor growth and metastasis,making in vivo high-resolution imaging of Cu(II)crucial for understanding its role in tumor pathophysiology.However,designing suitable molecular probes fo...Cu(II)ions play a critical role in tumor growth and metastasis,making in vivo high-resolution imaging of Cu(II)crucial for understanding its role in tumor pathophysiology.However,designing suitable molecular probes for this purpose remains challenging.Herein,we report the development of a photoacoustic probe for specific in vivo imaging of Cu(II)in tumors.This probe utilizesβ-galactoside as a targeting group and incorporates a unique self-immobilization strategy.Uponβ-galactosidase-mediated cleav-age,the probe generates a reactive quinone methide intermediate that covalently binds to intracellular proteins,enabling selective tumor accumulation.The probe exhibits a ratiometric photo-acoustic response to Cu(II)with high selectivity over that of other biological species.In vitro and in vivo studies demonstrated the efficacy of the probe for Cu(II)imaging in tumors.This research provides valuable insights into the role of Cu(II)in tumorigenesis and may facilitate the development of diagnostic and therapeutic approaches for cancer.展开更多
Formaldehyde(FA,a typical reactive carbonyl species)is a well-known environmental pollutant and a disease-related biomarker,making its sensitive and selective detection significant.Fluorescent probes have been explore...Formaldehyde(FA,a typical reactive carbonyl species)is a well-known environmental pollutant and a disease-related biomarker,making its sensitive and selective detection significant.Fluorescent probes have been explored for FA perception in environment,intracellular media and in vivo.In this review,we majorly conclude the recently represented fluorescence FA analysis based on small molecule probes.The general FA sensing mechanisms are first introduced.Regarding the FA detection in various environments,sensing tactics and performances are discussed in order of natural environment,living cells and in vivo.In the end,this review discusses the challenges and future trends of FA detection based on fluorescent probes.展开更多
Molecular imaging allows the biological processes taking place in the body to be viewed at a cellular and molecular level.Molecular imaging probe is used to visualize,characterize and quantify the processes.Nano-assem...Molecular imaging allows the biological processes taking place in the body to be viewed at a cellular and molecular level.Molecular imaging probe is used to visualize,characterize and quantify the processes.Nano-assembly small molecule probe provides an attractive means to precisely engineer nanomedicine with distinct biophysicochemical properties and simplify the optimization of formulation,which is an emerging and promising area that can integrate the advantages of the two kinds of molecular imaging probes with high loading capacity,effective signal amplification,preferable clearance,etc.Herein,we review main advances such as small molecules self-assembly into nanoprobes,its development and application,including fluorescent,magnetic resonance imaging,and nuclide marker labeled nano-assembly small molecules probes,as well as“smart”probes of this kind and their applications in molecular imaging.展开更多
基金supported by the Pre-Research Fund Project(BQ0302)
文摘Probe molecule Eu(DBM)3 phen is made up of europium oxide(EuO),dibenzoylmethane(DBM) and1,10-phenanthroline(phen). The temperature sensitive paint(TSP) was compounded by the polymerization of the probe molecule, methyl methacrylate(MMA) and the initiator of benzoyl peroxide(BPO).The structure, morphology, luminescence property of probe molecule and the temperature quenching property of the temperature sensitive paint(TSP) were characterized by infrared spectrometer, UV-vis spectrometer, scanning electron microscopy and fluorescence spectrometer respectively. The infrared spectrum and UV-vis spectra show that Eu and DBM form six membered rings, and Eu-O coordinate bonds form. The nanocrystals are in sphere-like morphology with an average size of approximately100 nm. Fluorescence spectra present that the performance of temperature quenching is excellent,what’s more, TSP sample has different temperature sensitivity in various temperature scope. Particularly,under excitation of 286 nm, TSP has a highest temperature sensitivity between 50 and 60 ℃, and the strongest fluorescence emission reaches a peak(615 nm). It indicated that probe molecule(Eu(DBM)phen) has strong luminescent intensity and the temperature quenching properties of Eu(DBM)phen/PMMA is good.
基金financially supported by the National Natural Science Foundation of China (Nos. 81672508 and 61505076)Jiangsu Provincial Foundation for Distinguished Young Scholars (No. BK20170041)+2 种基金Jiangsu Key Research and Development Program (No. BE2015699)Natural Science Foundation of Guangdong Province (No. 2017A030313299)State Key Laboratory of Pulp and Paper Engineering (No. 201706)
文摘Nitroreductase（NTR） is a member of flavin-containing enzymes that exists widely in bacteria. Hypoxia,which is a characteristic of locally advanced solid tumors, resulting from an imbalance between oxygen consumption and supply, can result in NTR overexpression. Using either nicotinamide adenine dinucleotide（NADH） or nicotinamide adenine dinucleotide phosphate（NADPH） as a source of reducing equivalents, NTR can catalyze the reduction of nitroaromatic compounds to the corresponding amines.Based on this reduction mechanism, NTR can be applied not only in the bioremediation and degradation of organic nitrogen compounds, but also in the development of NTR-targeted fluorescent probes to detect the hypoxic status of cancer cells. This review aims to provide a summary of the progress in fluorescent probes for NTR in recent years and elucidate the main fluorescent mechanisms that have been applied to design probes.
基金National Natural Science Foundation of China(22377057,22371124,22293050,and 22293051)the Excellent Research Program of Nanjing University(ZYJH004)the Natural Science Foundation of Jiangsu Province(BK20231298,BK20221333,and BK20232020).
文摘Cu(II)ions play a critical role in tumor growth and metastasis,making in vivo high-resolution imaging of Cu(II)crucial for understanding its role in tumor pathophysiology.However,designing suitable molecular probes for this purpose remains challenging.Herein,we report the development of a photoacoustic probe for specific in vivo imaging of Cu(II)in tumors.This probe utilizesβ-galactoside as a targeting group and incorporates a unique self-immobilization strategy.Uponβ-galactosidase-mediated cleav-age,the probe generates a reactive quinone methide intermediate that covalently binds to intracellular proteins,enabling selective tumor accumulation.The probe exhibits a ratiometric photo-acoustic response to Cu(II)with high selectivity over that of other biological species.In vitro and in vivo studies demonstrated the efficacy of the probe for Cu(II)imaging in tumors.This research provides valuable insights into the role of Cu(II)in tumorigenesis and may facilitate the development of diagnostic and therapeutic approaches for cancer.
基金supported by the National Natural Science Foundation of China(22074005)the Natural Science Foundation of Beijing Municipality(2202038)the Open Research Fund Program of Beijing Key Lab of Plant Resource Research and Development,Beijing Technology and Business University(PRRD-2021-YB6)
文摘Formaldehyde(FA,a typical reactive carbonyl species)is a well-known environmental pollutant and a disease-related biomarker,making its sensitive and selective detection significant.Fluorescent probes have been explored for FA perception in environment,intracellular media and in vivo.In this review,we majorly conclude the recently represented fluorescence FA analysis based on small molecule probes.The general FA sensing mechanisms are first introduced.Regarding the FA detection in various environments,sensing tactics and performances are discussed in order of natural environment,living cells and in vivo.In the end,this review discusses the challenges and future trends of FA detection based on fluorescent probes.
基金supported by the National Basic Research Program of China(2015CB931800)National Natural Science Foundation of China(81627901,31210103913)the Key Laboratory of Molecular Imaging Foundation(College of Heilongjiang Province).
文摘Molecular imaging allows the biological processes taking place in the body to be viewed at a cellular and molecular level.Molecular imaging probe is used to visualize,characterize and quantify the processes.Nano-assembly small molecule probe provides an attractive means to precisely engineer nanomedicine with distinct biophysicochemical properties and simplify the optimization of formulation,which is an emerging and promising area that can integrate the advantages of the two kinds of molecular imaging probes with high loading capacity,effective signal amplification,preferable clearance,etc.Herein,we review main advances such as small molecules self-assembly into nanoprobes,its development and application,including fluorescent,magnetic resonance imaging,and nuclide marker labeled nano-assembly small molecules probes,as well as“smart”probes of this kind and their applications in molecular imaging.
