Ischemic stroke is on the rise worldwide,and stent intervention is gradually becoming one of the effective treatments.Biodegradable Mg-Zn-Y-Nd alloy(ZE21B)has good mechanical properties and biocompatibility,and has a ...Ischemic stroke is on the rise worldwide,and stent intervention is gradually becoming one of the effective treatments.Biodegradable Mg-Zn-Y-Nd alloy(ZE21B)has good mechanical properties and biocompatibility,and has a good application prospect in vascular scaffolds.However,it still suffers from poor corrosion resistance,insufficient endothelialization,and blood-brain barrier(BBB)remodeling.Previously,it was found that constructing a bionic coating of barnacle gum protein cp19k on the surface of ZE21B by electrostatic spraying could improve its corrosion resistance and pro-endothelialization ability.Therefore,we considered piggybacking sulfonated hyaluronic acid nanoparticles(NP@S-HA)on the coating,which inherited the excellent anticoagulant and anti-inflammatory properties of S-HA on the basis of its improved corrosion resistance.The coatings were evaluated by electrochemistry,static immersion corrosion,in vitro blood experiments,and cellular experiments.Collectively,the cp19k/NP@S-HA coating demonstrated an average corrosion resistance enhancement of approximately 40.6%relative to the uncoated ZE21B.Cp19k/NP@S-HA was able to effectively improve the proliferation and migration of endothelial cells,inhibit the proliferation and regulate the contractile phenotype of smooth muscle cells,inhibit macrophage(MA)adherence,regulate the M2 phenotype of MAs,reduce the expression of the inflammatory factor tumor necrosis factor-α(TNF-α),and inhibit fibroplasia.In summary,the composite coatings developed in this study provide an effective strategy for surface modification of magnesium alloys in cerebrovascular applications,which has a broad application prospect.展开更多
During interventional surgeries of implantable cardiovascular devices in addressing cardiovascular diseases(CVD),the inevitable tissue damage will trigger host inflammation and vascular lumen injury,leading to delayed...During interventional surgeries of implantable cardiovascular devices in addressing cardiovascular diseases(CVD),the inevitable tissue damage will trigger host inflammation and vascular lumen injury,leading to delayed re-endothelization and intimal hyperplasia.Endowing cardiovascular implants with anti-inflammatory and endothelialization functions is conducive to the target site,offering significant tissue repair and regeneration benefits.Herein,inspired by the snake’s molting process,a ShedWise device was developed by using the poly(propylene fumarate)polyurethane(PPFU)as the foundational material,which was clicked with hyperbranched polylysine(HBPL)and followed by conjugation with pro-endothelial functional Arg-Glu-Asp-Val peptide(REDV),and finally coated with a“self-sacrificing”layer having reactive oxygen species(ROS)-scavenging ability and degradability.During the acute inflammation in the initial stage of implantation,the ROS-responsive hyperbranched poly acrylate-capped thioketone-containing ethylene glycol(HBPAK)coating effectively modulated the level of environmental inflammation and resisted initial protein adsorption,showcasing robust tissue protection.As the coating gradually“sacrificed”,the exposed hyperbranched HBPL-REDV layer recruited specifically endothelial cells and promoted surface endothelialization.In a rat vascular injury model,the ShedWise demonstrated remarkable efficiency in reducing vascular restenosis,protecting the injured tissue,and fostering re-endothelization of the target site.This innovative design will introduce a novel strategy for surface engineering of cardiovascular implants and other medical devices.展开更多
基金supported by the National Key Research and Development Program of China(2021YFC2400703)Zhengzhou University Major Project Cultivation Special Project(125-32214076)。
文摘Ischemic stroke is on the rise worldwide,and stent intervention is gradually becoming one of the effective treatments.Biodegradable Mg-Zn-Y-Nd alloy(ZE21B)has good mechanical properties and biocompatibility,and has a good application prospect in vascular scaffolds.However,it still suffers from poor corrosion resistance,insufficient endothelialization,and blood-brain barrier(BBB)remodeling.Previously,it was found that constructing a bionic coating of barnacle gum protein cp19k on the surface of ZE21B by electrostatic spraying could improve its corrosion resistance and pro-endothelialization ability.Therefore,we considered piggybacking sulfonated hyaluronic acid nanoparticles(NP@S-HA)on the coating,which inherited the excellent anticoagulant and anti-inflammatory properties of S-HA on the basis of its improved corrosion resistance.The coatings were evaluated by electrochemistry,static immersion corrosion,in vitro blood experiments,and cellular experiments.Collectively,the cp19k/NP@S-HA coating demonstrated an average corrosion resistance enhancement of approximately 40.6%relative to the uncoated ZE21B.Cp19k/NP@S-HA was able to effectively improve the proliferation and migration of endothelial cells,inhibit the proliferation and regulate the contractile phenotype of smooth muscle cells,inhibit macrophage(MA)adherence,regulate the M2 phenotype of MAs,reduce the expression of the inflammatory factor tumor necrosis factor-α(TNF-α),and inhibit fibroplasia.In summary,the composite coatings developed in this study provide an effective strategy for surface modification of magnesium alloys in cerebrovascular applications,which has a broad application prospect.
基金financially supported by the Joint Fund of National Natural Science Foundation of China(U22A20155)the Lingyan Program of Zhejiang Province(2022C01106)+1 种基金the State Key Laboratory of Transvascular Implantation Devices(012024004)the ZJU DPSE-NEWMED DPSE-NEWMED Biomedical Polymers and Devices Research&Development Lab,and the 111 Project(B16042).
文摘During interventional surgeries of implantable cardiovascular devices in addressing cardiovascular diseases(CVD),the inevitable tissue damage will trigger host inflammation and vascular lumen injury,leading to delayed re-endothelization and intimal hyperplasia.Endowing cardiovascular implants with anti-inflammatory and endothelialization functions is conducive to the target site,offering significant tissue repair and regeneration benefits.Herein,inspired by the snake’s molting process,a ShedWise device was developed by using the poly(propylene fumarate)polyurethane(PPFU)as the foundational material,which was clicked with hyperbranched polylysine(HBPL)and followed by conjugation with pro-endothelial functional Arg-Glu-Asp-Val peptide(REDV),and finally coated with a“self-sacrificing”layer having reactive oxygen species(ROS)-scavenging ability and degradability.During the acute inflammation in the initial stage of implantation,the ROS-responsive hyperbranched poly acrylate-capped thioketone-containing ethylene glycol(HBPAK)coating effectively modulated the level of environmental inflammation and resisted initial protein adsorption,showcasing robust tissue protection.As the coating gradually“sacrificed”,the exposed hyperbranched HBPL-REDV layer recruited specifically endothelial cells and promoted surface endothelialization.In a rat vascular injury model,the ShedWise demonstrated remarkable efficiency in reducing vascular restenosis,protecting the injured tissue,and fostering re-endothelization of the target site.This innovative design will introduce a novel strategy for surface engineering of cardiovascular implants and other medical devices.
