Recent advances in organ transplantation,regenerative medicine,and drug discovery have emphasized the critical importance of effective preservation techniques for organs.Despite these advances,current preservation tec...Recent advances in organ transplantation,regenerative medicine,and drug discovery have emphasized the critical importance of effective preservation techniques for organs.Despite these advances,current preservation techniques have significant limitations in maintaining the viability and functional efficacy of organs over the long term.As a result,there is a pressing need to develop reliable and efficient preservation strategies for organs.Currently,the clinical standard for organ preservation involves the use of static cold storage and organ machine perfusion,but these methods can only preserve organs for a couple of days or even a few hours.Notably,the development of cryobiology has yielded promising alternatives.In this review,we aim to provide a comprehensive overview of the progression of organ preservation methods,while emphasizing the limitations of traditional approaches.Moreover,we evaluate advanced preservation techniques for organs,including kidneys,livers,hearts,lungs,and intestines.Furthermore,we share a progress perspective on the future of organ preservation,with the ultimate goal of achieving viable long-term preservation to address the pressing issue of organ shortage.展开更多
Fertility preservation and pregnancymanagement are critical considerations for patients undergoing organtransplantation.Innovations in assisted reproductive technologies,hormonalmodulation,and personalized medicine ha...Fertility preservation and pregnancymanagement are critical considerations for patients undergoing organtransplantation.Innovations in assisted reproductive technologies,hormonalmodulation,and personalized medicine have expanded options for these patients,who face unique challenges due to immunosuppressive therapy and organ functionconcerns.This mini-review explores advancements in cryopreservationtechniques,pre-conception counseling,and multidisciplinary strategies forsafe pregnancies post-transplantation.Emphasis is placed on balancing maternalhealth,graft function,and fetal outcomes.The integration of reproductive andtransplant medicine is paving the way for improved quality of life andreproductive autonomy for this patient population.展开更多
In addition to the organic matter type,abundance,thermal maturity,and shale reservoir space,the preservation conditions of source rocks play a key factor in affecting the quantity and quality of retained hydrocarbons ...In addition to the organic matter type,abundance,thermal maturity,and shale reservoir space,the preservation conditions of source rocks play a key factor in affecting the quantity and quality of retained hydrocarbons in source rocks of lacustrine shale,yet this aspect has received little attention.This paper,based on the case analysis,explores how preservation conditions influence the enrichment of mobile hydrocarbons in shale oil.Research showns that good preservation conditions play three key roles.(1)Ensure the retention of sufficient light hydrocarbons(C_(1)–C_(13)),medium hydrocarbons(C_(14)–C_(25))and small molecular aromatics(including 1–2 benzene rings)in the formation,which enhances the fluidity and flow of shale oil;(2)Maintain a high energy field(abnormally high pressure),thus facilitating the maximum outflow of shale oil;(3)Ensure that the retained hydrocarbons have the miscible flow condition of multi-component hydrocarbons(light hydrocarbons,medium hydrocarbons,heavy hydrocarbons,and heteroatomic compounds),so that the heavy hydrocarbons(C_(25+))and heavy components(non-hydrocarbons and asphaltenes)have improved fluidity and maximum flow capacity.In conclusion,in addition to the advantages of organic matter type,abundance,thermal maturity,and reservoir space,good preservation conditions of shale layers are essential for the formation of economically viable shale oil reservoirs,which should be incorporated into the evaluation criteria of shale oil-rich areas/segments and considered a necessary factor when selecting favorable exploration targets.展开更多
In this study,composite films consisting of polylactic acid(PLA),ethyl cellulose(EC),and zein were prepared by solution casting method,and their performance and application in chilled fresh meat preservation were inve...In this study,composite films consisting of polylactic acid(PLA),ethyl cellulose(EC),and zein were prepared by solution casting method,and their performance and application in chilled fresh meat preservation were investigated.The results showed that the three materials had satisfactory compatibility in the composite film.Addition of EC and zein effectively improved the mechanical properties,thermodynamic properties,surface hydrophilicity,oxygen permeability,and degradation properties of PLA films.When the ratio of PLA to EC was 3:7,the tensile strength and elongation at break reached maximum values of 16.6 MPa and 30.5%,respectively.Moreover,under different conditions,the composite film exhibited better degradability than the PLA film.The composite film with a 3:7 ratio of PLA to EC had the best performance,with a degradation rate of 21.75%after 84 days.Chilled fresh meat wrapped with the composite film showed significantly improved antioxidant,antibacterial,and water-holding properties.展开更多
To alleviate the quality deterioration and extend the shelf life of crucian carp fillets,a bio-based active composite film integrating eugenol(EG)-loaded mesoporous silica nanoparticles(MSNs)modified with caffeic acid...To alleviate the quality deterioration and extend the shelf life of crucian carp fillets,a bio-based active composite film integrating eugenol(EG)-loaded mesoporous silica nanoparticles(MSNs)modified with caffeic acid(CA)within the zein matrix(EG/CA-MSN/Zein)was developed.This study compared the effects of EG/CA-MSN/Zein,polyethylene(PE),and zein films on the quality of fish fillets during refrigerated storage.The results showed that the EG/CA-MSN/Zein film significantly retarded the increase in pH and microbial growth.Moreover,the film had higher water-holding capacity(WHC),better texture,and color stability,as indicated by low-field nuclear magnetic resonance(LF-NMR),magnetic resonance imaging(MRI),and sensory evaluation.The EG/CA-MSN/Zein composite film extended the shelf life of crucian carp fillets by 3-4 days,highlighting its potential as an environmentally friendly and efficient packaging material for aquatic products.展开更多
Mold spoilage and nutrition enrichment are major concerns of Chinese steamed bread,and using functional lactic acid bacteria as starter to play the role of in-situ preservation and nutritional fortification was a prom...Mold spoilage and nutrition enrichment are major concerns of Chinese steamed bread,and using functional lactic acid bacteria as starter to play the role of in-situ preservation and nutritional fortification was a promising alternative.Hence,Lactiplantibacillus plantarum P10 capable of inhibiting spoilage microorganisms and producing γ-aminobutyric acid(GABA)(380 mg/L)was screened from Chinese pickle.Metabolomics analysis showed that P10 produced key antimicrobial metabolites mainly including ten organic acids,aldehyde,alkaloid.Besides,P10 might exhibit health beneficial effects such as degrading cholesterol,lowering blood glucose and reducing uric acid due to the production of deoxycholic acid and Ile-Pro-Ile,and the degradation of purine.Finally,the promising application potential of P10 was evidenced in steamed bread with extended shelf life,excellent anti-aging effect,and 24.5-fold enrichment of GABA.Conclusively,this investigation could provide scientific basis for the application of P10 as a functional starter in steamed bread.展开更多
The thioacetamide derivative(TD)-composite preservation system(TDCPS)exhibits superior preservation effects on natural rubber latex(NRL)and significantly enhances its vulcanization efficiency and mechanical properties...The thioacetamide derivative(TD)-composite preservation system(TDCPS)exhibits superior preservation effects on natural rubber latex(NRL)and significantly enhances its vulcanization efficiency and mechanical properties.This study primarily investigated the principal chemical groups and mechanism of action of TDCPS in promoting NRL vulcanization through a comparative analysis.The results indicated that the key functional groups(thioamide and pyridine)in TDCPS synergistically accelerated crosslinking,reducing the vulcanization time by 41.18%compared to the high-ammonia(HA)preservation system.At an optimal TDCPS dosage of 5 mmol·L^(−1),vulcanized films achieved a tensile strength of 34.18 MPa,with a sulfur content of 1.5 phr further improving the strength by 42.26%.TD outperformed the conventional accelerators 2-imidazolidinethione(ETU)and 3-hydroxypyridine(3-Hp)in promoting the crosslinking density and mechanical performance while eliminating ammonia-related environmental risks.This eco-friendly system demonstrates the industrial potential for sustainable rubber production.展开更多
In order to ensure food safety,controlling foodborne pathogen contamination is of utmost importance.Growing apprehensions regarding the safety of synthetic antimicrobials,due to their adverse health effects,have promp...In order to ensure food safety,controlling foodborne pathogen contamination is of utmost importance.Growing apprehensions regarding the safety of synthetic antimicrobials,due to their adverse health effects,have prompted a search for alternative options.Plant natural products(PNPs)with antimicrobial activity are being explored as a viable alternative.Among the various antimicrobial natural products studied,plant essential oils,plant flavonoids,plant polyphenols,plant polysaccharides,and plant antimicrobial peptides have been identified as potential candidates.PNPs demonstrate a diverse array of antimicrobial mechanisms,encompassing cell wall and membrane damage,interference with genetic replication,disruption of energy metabolism,and induction of oxidative stress at the single-cell level,as well as inhibition of biofilm formation and quorum sensing at the population level.Certain PNPs have been harnessed as natural antimicrobial agents for the food preservation.The utilization of encapsulation technology proves to be an effective strategy in protecting PNPs,thereby ensuring good antimicrobial efficacy,enhanced dispersibility,and controlled release within food products.The utilization of nanoemulsions,nanoliposomes,edible packaging,electrospun nanofibers,and microcapsules formed by encapsulation has enriched the ways in which PNPs can be applied in food preservation.Although PNPs have great potential in food preservation,their widespread application in the food industry is currently constrained by factors such as production costs,safety concerns,and legal considerations.Chemical synthesis and biosynthesis pathways offer viable strategies for reducing the cost of producing PNPs,and ongoing efforts to assess safety and improve regulatory frameworks are likely to facilitate the broader adoption of PNPs in food preservation practices.This article provides an overview of the main types of PNPs with antimicrobial activity and their properties,focusing on their mechanisms of action.Additionally,it summarizes the use of PNPs in food preservation and discusses the characteristics and applications of different encapsulation technologies.Lastly,the paper briefly analyzes current limitations and proposes potential future trends for this field.展开更多
BACKGROUND Low rectal cancer poses a significant surgical challenge because of its close proximity to the anal sphincter,often requiring radical resection with permanent colostomy to achieve oncological safety.Revisit...BACKGROUND Low rectal cancer poses a significant surgical challenge because of its close proximity to the anal sphincter,often requiring radical resection with permanent colostomy to achieve oncological safety.Revisited rectal anatomy,advances in surgical techniques and neoadjuvant therapies have enabled the possibility of sphincter-preserving procedures,however,it is uniformly not applicable.Selecting appropriate candidates for sphincter preservation is crucial,as an illadvised approach may compromise oncological outcome or lead to poor functional outcomes.Currently there is no consensus-which clinical,anatomical,or molecular factors most accurately predict the feasibility of sphincter-preserving surgery(SPS)in this subset of patients.By identifying these predictors,the study seeks to support improved patient selection,enhance surgical planning,and ultimately contribute to better functional and oncological outcomes in patients with low rectal cancer.AIM To identify predictive factors that determine the feasibility of SPS in patients with low rectal cancer.METHODS A comprehensive literature search was conducted using PubMed/MEDLINE databases.The search focused on various factors influencing the feasibility of SPS in low rectal cancer.These included patient-related factors,anatomical considerations,findings from different imaging modalities,advancements in diagnostic tools and techniques,and the role of neoadjuvant chemoradiotherapy.The relevance of each factor in predicting the potential for sphincter preservation was critically analyzed and presented based on the current evidence RESULTS Multiple studies have identified a range of predictive factors influencing the feasibility of SPS in low rectal cancer.Patient-related factors include age,sex,preoperative continence status,comorbidities,and body mass index.Anatomical considerations,such as tumor distance from the anal verge,involvement of the external anal sphincter,and levator ani muscles,also play a critical role.Additionally,a favourable response to neoadjuvant chemoradiotherapy has been associated with improved suitability for sphincter preservation.Several biomarkers,such as inflammatory markers like interleukins and C-reactive protein,as well as tumor markers like carcinoembryonic antigen,are important.Molecular markers,including BRAF and KRAS mutations and microsatellite instability status,have been linked to prognosis and may further guide decision-making regarding sphincter-preserving approaches.Artificial intelligence(AI)can further add in to select an ideal patient for sphincter preservation.CONCLUSION SPS is feasible in low rectal cancer and depends on patient factors,tumor anatomy and biology,preoperative treatment response,and biomarkers.In addition,tools and technology including AI can further help in selecting an ideal patient for long term optimal outcome.展开更多
BACKGROUND The surgical management of rectal cancer is continuously advancing,with a current emphasis on minimising the need for a permanent stoma.Understanding the risk factors influencing sphincter preservation is c...BACKGROUND The surgical management of rectal cancer is continuously advancing,with a current emphasis on minimising the need for a permanent stoma.Understanding the risk factors influencing sphincter preservation is crucial for guiding clinical decision-making and optimising preoperative patient evaluation.AIM To examine the risk factors influencing sphincter preservation in laparoscopic radical rectal cancer surgery.METHODS A retrospective analysis of the demographics,preoperative and intraoperative data,and pathological findings of 179 patients with rectal cancer who underwent laparoscopic radical rectal cancer surgery at our hospital between January 2022 and December 2023 was conducted.These clinical data were compared between two groups:Patients with sphincter preservation and those without,categorised as the sphincter-preserved and sphincter-unpreserved groups,respectively.RESULTS Of the 179 patients analysed,150 were in the sphincter-preserved group and 29 were in the sphincter-unpreserved group.Tumour height was significantly greater in the sphincter-preserved group compared to the sphincter-unpreserved group.Conversely,elevated levels of carcinoembryonic antigen,carbohydrate antigen 19-9,and plasma D-dimer were significantly higher in the sphincter-unpreserved group.Significant differences were also observed between the two groups in terms of place of residence,presence of colonic polyps,neoadjuvant chemotherapy,preoperative radiotherapy,mucinous adenocarcinoma,nerve invasion,and tumour height.No significant differences were observed for other parameters.Logistic regression analysis identified colonic polyps,mucinous adenocarcinoma,nerve invasion,and tumour height as independent risk factors for sphincter preser-vation.CONCLUSION Several risk factors influencing sphincter preservation in laparoscopic radical rectal cancer surgery were identified.These factors could be valuable tools for guiding clinical decision-making and optimising preoperative patient evaluations.展开更多
The Yangchuling porphyry W-Mo deposit(YPWD),located in the Jiangnan porphyryskarn tungsten ore belt,is one of the most important and large-scale porphyry W-Mo deposits in South China.While previous zircon U-Pb and mol...The Yangchuling porphyry W-Mo deposit(YPWD),located in the Jiangnan porphyryskarn tungsten ore belt,is one of the most important and large-scale porphyry W-Mo deposits in South China.While previous zircon U-Pb and molybdenite Re-Os data suggest that Yangchuling WMo ore bodies formed almost simultaneously with granodiorite and monzogranitic porphyry at~150–144 Ma,their post emplacement history remains poorly understood,making their preservation status at depth uncertain.In this paper,new zircon and apatite(U-Th)/He and apatite fission track(ZHe,AHe and AFT,respectively)data of one hornfels and five intrusive rocks from a 1000-meter borehole are presented.These,together with new inverse thermal history models and previous geochronological data,help elucidate the post-diagenetic exhumation history and preservation status of the Yangchuling porphyry W-Mo deposit.In general,ZHe and AHe ages decrease gradually from the near surface downwards and have relatively little intra-sample variation,ranging from 133 to 73Ma and 67 to 25 Ma,respectively.All four granodiorites yield similar AFT ages that range from 63 to 55 Ma with mean track lengths varying from 12.2±0.7 to 12.6±0.5μm.Thermal history modelling indicates that the Yangchuling ore district experienced slow,monotonic cooling since the Cretaceous.Age-depth relationships are interpreted as recording~3.7±0.8 km of Cretaceous-recent exhumation in response to regional extension throughout South China thought to have been driven by subduction retreat of the Paleo-Pacific Plate.Comparison of estimated net exhumation and previous metallogenic depth of~4–5 km suggests that W-Mo ore bodies could still exist at depths of up to~1.3±0.8 km relative to Earth surface in the YPWD region.Preservation of the YPWD is attributed to the limited amount of regional denudation during the Late Cretaceous and Cenozoic.展开更多
The aim of this investigation was to determine the optimal storage medium for testicular hypothermic transportation and identify the ideal concentration for the application of the protective agent 5-aminolevulinic aci...The aim of this investigation was to determine the optimal storage medium for testicular hypothermic transportation and identify the ideal concentration for the application of the protective agent 5-aminolevulinic acid(5-ALA).Furthermore,this study aimed to explore the underlying mechanism of the protective effects of 5-ALA.First,we collected and stored mouse testicular fragments in different media,including Hank’s balanced salt solution(HBSS;n=5),Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12(DMEM/F12;n=5),and alpha-minimum essential medium(αMEM;n=5).Storage of testicular tissue in HBSS preserved the integrity of testicular morphology better than that in the DMEM/F12 group(P<0.05)and theαMEM group(P<0.01).Testicular fragments were subsequently placed in HBSS with various concentrations of 5-ALA(0[control],1 mmol l−1,2 mmol l−1,and 5 mmol l−1)to determine the most effective concentration of 5-ALA.The 2 mmol l−15-ALA group(n=3)presented the highest positive rate of spermatogonial stem cells compared with those in the control,1 mmol l−1,and 5 mmol l−15-ALA groups.Finally,the tissue fragments were preserved in HBSS with control(n=3)and 2 mmol l−15-ALA(n=3)under low-temperature conditions.A comparative analysis was performed against fresh testes(n=3)to elucidate the underlying mechanism of 5-ALA.Gene set enrichment analysis(GSEA)for WikiPathways revealed that the p38 mitogen-activated protein kinase(MAPK)signaling pathway was downregulated in the 2 mmol l−15-ALA group compared with that in the control group(normalized enrichment score[NES]=−1.57,false discovery rate[FDR]=0.229,and P=0.019).In conclusion,these data suggest that using 2 mmol l−15-ALA in HBSS effectively protected the viability of spermatogonial stem cells upon hypothermic transportation.展开更多
Occurrence and abundance of molecular hydrogen in natural geologic reservoirs are enigmatic,due to its various sources,diverse migration pathways and complicated biological and chemical reactions.Natural gas samples c...Occurrence and abundance of molecular hydrogen in natural geologic reservoirs are enigmatic,due to its various sources,diverse migration pathways and complicated biological and chemical reactions.Natural gas samples containing hydrogen from producing wells in several sedimentary basins in China were collected in this study,and gas abundances and isotopic compositions of these gases were compared with those in global petroliferous basins and deep intrusive rocks.Several geochemical indicators were suggested for identifying sources,migration and accumulation mechanisms of hydrogen in the subsurface environment.Hydrogen contents in natural gas deposits have contributions from various sources with the following high-to-low order:microbial degradation>serpentinization>deep mantle volatile release>radiation-induced water decomposition>thermal cracking of organic matter.A hydrogen-rich reservoir in Kansas,USA,is specifically analyzed to determine its formation mechanism.This study suggests that future exploration of geological hydrogen resources may focus on the igneous rock bodies with overlying dense sedimentary rocks in the continental rift systems.展开更多
BACKGROUND Osteonecrosis of the femoral head(ONFH)is a prevalent clinical condition,and as the affected population becomes younger and more widespread,an increasing number of patients prefer to retain their own hip jo...BACKGROUND Osteonecrosis of the femoral head(ONFH)is a prevalent clinical condition,and as the affected population becomes younger and more widespread,an increasing number of patients prefer to retain their own hip joints.In comparison to joint replacement and hip-preserving surgeries,conservative hip preservation treatment is gaining more recognition from both clinicians and patients.AIM To observe the clinical efficacy and influencing factors of conservative hip preservation treatment for patients with ONFH.METHODS A total of 73 patients(119 hips)were included,and patients were grouped based on Association Research Circulation Osseous(ARCO)stage,Japanese Investigation Committee(JIC)classification,lesion size,and collapse degree.The clinical efficacy of conservative hip preservation treatment was evaluated from two aspects:Radiological progression and clinical functional scores[Harris Hip Score,Western Ontario and McMaster Universities Arthritis Index(WOMAC),international Hip Outcome Tool 12(iHOT-12),and visual analogue scale(VAS)].Analyzing the success rate of conservative hip preservation based on conversion to total hip arthroplasty or hip preservation surgery.RESULTS In this retrospective cohort study,we retrospectively analyzed 73 patients(119 hips)with an average follow-up time of 53.58±26.80(18-129)months.The overall success rate of hip preservation is 90.76%.The success rates of hip preservation in ARCO I-II,IIIa,and IIIb were 97.67%,91.53%,and 70.59%,respectively(P<0.05).The success rates of hip preservation for JIC A-B,C1,and C2 were 93.37%,92.59%,and 77.78%,respectively(P<0.05).The success rates of hip preservation with collapse degree≤2 mm and>2 mm were 94.12%and 70.59%,respectively(P<0.05).There was no significant difference in the success rate of hip preservation between different lesion sizes.In terms of clinical function,the Harris Hip Score,WOMAC,iHOT-12,and VAS scores at the last follow-up were significantly better than before treatment(P<0.05).The VAS scores of ARCO IIIa and IIIb were significantly worse than those of ARCO I and II(P<0.05).The iHOT-12,WOMAC,and VAS scores were significantly better with the lesion size<15%(P<0.05).In terms of radiological results,there was significant progress in ARCO staging and collapse degree after conservative hip preservation treatment,but there was no significant difference in JIC classification and lesion size.CONCLUSION Conservative hip preservation treatment can effectively treat ONFH,prevent the progression of the necrosis,and significantly improve the clinical function of patients.The radiological staging and lesion size at the initial visit may help determine the prognosis of conservative hip preservation treatment.展开更多
Porous organic polymers are remarkably versatile materials with porous and carefully designed structures.They complement traditional preservation methods by overcoming their limitations and significantly extending the...Porous organic polymers are remarkably versatile materials with porous and carefully designed structures.They complement traditional preservation methods by overcoming their limitations and significantly extending the shelf life of preserved products.Notably,porous hollow nanospheres(PHNs),with their unique hollow structures capable of adsorbing and releasing organic molecules,have garnered considerable attention in food preservation.However,most PHNs are challenging to synthesize in one step,and PHNs are usually in powder form,which makes it challenging to apply them directly.In this study,we successfully synthesized PHNs in one step using the Friedel–Crafts reaction.The PHNs,adsorbed with hexanal molecules,were then embedded in polymer fibers to create composites via electrospinning.The preservation effect of the composite nanofiber membranes was investigated by determining the changes in appearance,weight,peel hardness,and pulp sugar content of three fruits,namely strawberries,bananas,and kumquats,after several days of storage.In comparison to pure poly(ε-caprolactone)fiber membranes,PHNs containing hexanal molecules slowed down the oxidative deterioration process and enhanced the quality and flavor of preserved fruits.This research presents innovative approaches for using porous organic polymers in food preservation and serves as a valuable reference for the development of future food packaging materials.展开更多
In recent years,drilling data from wells Pengshen 10,Heshen 9,Tongshen 17 and Zhengyang 1 in the Sichuan Basin have confirmed the presence of a set of porous reef-beach limestone reservoirs in the Upper Permian Changx...In recent years,drilling data from wells Pengshen 10,Heshen 9,Tongshen 17 and Zhengyang 1 in the Sichuan Basin have confirmed the presence of a set of porous reef-beach limestone reservoirs in the Upper Permian Changxing Formation,which breaks the traditional view that deep carbonate oil and gas are only distributed in porous dolomite reservoirs and karst fracture-cavity limestone reservoirs.Through core and thin section observations,geochemical analysis,and well-seismic based reservoir identification and tracking,the study on formation mechanism of pores in deep reef-beach limestone reservoirs is carried out,this study provides insights in four aspects.(1)Porous reef-beach limestone reservoirs are developed in the Changxing Formation in deep-buried layers.The reservoir space is composed of intergranular pores,framework pores,intra-fossil pores,moldic pores and dissolution pores,which are formed in depositional and epigenetic environments.(2)The intermittently distributed porous reef-beach complexes are surrounded by relatively dense micrite limestone,which leads to the formation of local abnormal high-pressure inside the reef-beach complexes with the temperature increased.(3)The floor of the Changxing Formation reservoir is composed with interbedded tight mudstone and limestone of the Upper Permian Wujiaping Formation,and the roof is the tight micrite limestone interbedded with mudstone of the first member of Lower Triassic Feixianguan Formation.Under the clamping of dense roof and floor,the abnormal high-pressure in the Changxing Formation is formed.Abnormal high-pressure(overpressured compartment)is the key to maintain the pores formed in the depositional and epigenetic environments in deep-buried layers.(4)Based on the identification of roof,floor and reef-beach complexes,the favorable reef-beach limestone reservoir distribution area of 10.3×10^(4) km^(2) is predicted by well-seismic integration.These insights lay the theoretical foundation for the development of deep porous limestone reservoirs,expand the new field of exploration of deep-buried limestone reservoirs in the Sichuan Basin.展开更多
Konjac is an ideal candidate for edible coatings on fruits due to its hydrophilic properties,film-forming ability,barrier properties,safety,and biodegradability.Meanwhile,the high market demand for strawberries necessi...Konjac is an ideal candidate for edible coatings on fruits due to its hydrophilic properties,film-forming ability,barrier properties,safety,and biodegradability.Meanwhile,the high market demand for strawberries necessitates post-harvest treatment to extend their shelf life and preserve their quality,as strawberries are known for their fragile skin and soft texture.To fully utilize konjac and develop high-quality coatingfilms,native konjacflour(NKF)and konjac glucomannan(KGM)were extracted from its corm and used as a coatingfilm for strawberries in the present study.Therefore,this study aimed to compare the physical properties of thefilm coatings between NKF and KGM,and evaluate their effects on strawberries preservation over 7 days of storage.A multistage extrac-tion process was employed to isolate NKF and KGM,after which the glucomannan content was measured.NKF yield was 31.81%,exceeding KGM yield of 26.42%,and the glucomannan content obtained of NKF(25.93%)was higher than KGM(21.41%).Nuclear magnetic resonance spectroscopy confirmed that both NKF and KGM con-tain glucomannan in their structure.Furthermore,both NKF and KGM were combined with carboxymethyl cel-lulose(CMC)and glycerol to produce eight thin-layerfilms to assess their physical and mechanical properties.Compared to the KGM variant,the NKF variant generally exhibited higher moisture content,water vapor trans-mission rate,and tensile strength.However,NKF was less effective than KGM in extending strawberry storage life,leading to faster color changes and greater weight loss,despite maintaining similar hardness values.Nonetheless,konjac-based coatings were generally effective at maintaining the freshness and quality of strawberries compared to uncoated samples.Konjac shows promise as an edible coating,improving fresh produce shelf life and appeal,aligning with consumer preferences for natural and sustainable products.展开更多
In 2004 and 2014,two monographs on hypobaric storage(LP)were published by Stanley P.Burg.Based on his theoretical framework and technological advancements,as well as the research and development of equipment conducted...In 2004 and 2014,two monographs on hypobaric storage(LP)were published by Stanley P.Burg.Based on his theoretical framework and technological advancements,as well as the research and development of equipment conducted under his guidance,alongside customer practices and reflections on various scientific literature both domestically and internationally,it is posited that,as articulated in the monograph,misconceptions regarding his theory and technology in Western scientific literature prior to 1985 continue to persist.This ongoing dissemination of misunderstandings has resulted in a near stagnation of research and has adversely impacted the Chinese academic community as well.Consequently,it is essential to delineate the characteristics and main points of its theory and technology,with the aim of offering guidance to individuals seeking to comprehend its foundational purpose.LP technology is a dynamic physical technology that continuously and uninterruptedly extracts air from a closed container and simultaneously introduces fresh,low-pressure moist air from the external environment,while maintaining specific levels of humidity and/or temperature within the container and upholding a predetermined pressure value.Preservation technology is the collective term for the set of various technical parameters associated with preservation,including pressure,relative humidity,and other relevant factors,to which LP equipment is specifically designed.The theory of LP is characterized by the enhanced diffusion of gases and vapors that enter and exit the commodity in a dynamic manner under low pressure conditions.The theoretical points involve equipment performance,low pressure,the impact of trace concentrations of gases such as O_(2),CO_(2) and C_(2)H_(4) that naturally occur at low pressure,diffusive mass transfer,heat transfer,and impacts on the activity of enzymes associated with maturation and senescence.The technology is characterized by dynamic low pressure,and the range of commodities preserved is comparable to that of refrigeration.However,certain commodities exiting the hypobaric environment possess subsequent preservation advantages that are not available through refrigeration.The main points of the technology encompass an extended sotrage life,a postponement of quality degradation,minimized water loss,the suppression of pathogen growth,and the killing of both internal and external insects of the commodity under dynamic low pressure conditions.The core advantage of LP technology lies in its ability to significantly reduce water loss,inhibit respiration and C_(2)H_(4) action,and pathogen growth,killing insects and modulate the activity of enzymes associated with maturation and senescence in post-harvest fresh horticultural products.Consequently,this technology plays a crucial role in prolonging the post-harvest lifespan of these commodities and mitigating quality degradation.Over the past decade,researchers in China have developed a hypobaric short period treatment technology,grounded in LP theory and technical practice,which is commonly referred to as hypobaric treatment.This method has garnered significant attention,leading to an increase in both domestic and international research.A growing body of literature categorizes LP as hypobaric treatment,while some studies also consider vacuum packaging and modified atmosphere packaging(MAP)as LP or hypobaric treatment.Misunderstandings are exacerbated by confusion surrounding nomenclature,which,in conjunction with pre-existing misconceptions,represents a significant barrier to both the research and practical application of the technology.The successful commercial implementation of a vacuum cold fresh chain,centered on LP or hypobaric treatment technology,may be the sole solution to the prevailing misunderstandings associated with LP.展开更多
The 10000-m ultradeep dolomite reservoir holds significant potential as a successor field for future oil and gas exploration in China's marine craton basin.However,major challenges such as the genesis of dolomite,...The 10000-m ultradeep dolomite reservoir holds significant potential as a successor field for future oil and gas exploration in China's marine craton basin.However,major challenges such as the genesis of dolomite,the formation time of high-quality reservoirs,and the preservation mechanism of reservoirs have always limited exploration decision-making.This research systematically elaborates on the genesis and reservoir-forming mechanisms of Sinian-Cambrian dolomite,discussing the ancient marine environment where microorganisms and dolomite develop,which controls the formation of large-scale Precambrian-Cambrian dolomite.The periodic changes inMg isotopes and sedimentary cycles show that the thick-layered dolomite is the result of different dolomitization processes superimposed on a spatiotemporal scale.Lattice defects and dolomite embryos can promote dolomitization.By simulating the dissolution of typical calcite and dolomite crystal faces in different solution systems and calculating their molecular weights,the essence of heterogeneous dissolution and pore formation on typical calcite and dolomite crystal faces was revealed,and the mechanism of dolomitization was also demonstrated.The properties of calcite and dolomite(104)/(110)grain boundaries and their dissolution mechanism in carbonate solution were revealed,showing the limiting factors of the dolomitization process and the preservation mechanism of deep buried dolomite reservoirs.The in situ laser U-Pb isotope dating technique has demonstrated the timing of dolomitization and pore formation in ancient carbonate rocks.This research also proposed that dolomitization occurred during the quasi-contemporaneous or shallow-burial periods within 50Ma after deposition and pores formed during the quasi-contemporaneous to the early diagenetic periods.And it was clear that the quasi-contemporaneous dolomitization was the key period for reservoir formation.The systematic characterization of the spatial distribution of the deepest dolomite reservoirs in multiple sets of the Sinian and the Cambrian in the Chinese craton basins provides an important basis for the distribution prediction of large-scale dolomite reservoirs.It clarifies the targets for oil and gas exploration at depths over 10000 m.The research on dolomite in this study will greatly promote China's ultradeep oil and gas exploration and lead the Chinese petroleum industry into a new era of 10000-m deep oil exploration.展开更多
By analyzing the structure and academic characteristics of early Western Han medical texts such as Prescriptions for Fifty-Two Diseases(Wu Shi Er Bing Fang)and Prescriptions for Health Preservation(Yang Sheng Fang),an...By analyzing the structure and academic characteristics of early Western Han medical texts such as Prescriptions for Fifty-Two Diseases(Wu Shi Er Bing Fang)and Prescriptions for Health Preservation(Yang Sheng Fang),and considering the development of philosophical thinking during the pre-Qin and Han periods,this study summarizes how Zhongjing Zhang’s Treatise on Cold Damage and Miscellaneous Diseases(Shang Han Za Bing Lun)inherited pre-Qin and Han philosophical thought while further integrating Daoist“natural law”cosmology,Confucian“benevolence”social ethics,and the“isomorphic unity of heaven and humanity”medical theory in Yellow Emperor’s Inner Classic(Huang Di Nei Jing).It proposes health preservation concepts encompassing heaven-human,body-spirit,and benevolence perspectives,along with dietary hygiene,representing the medical embodiment of pre-Han“virtue cultivation”and“body nurturing”life philosophies that profoundly influenced subsequent traditional Chinese medicine health preservation theory and practice.展开更多
基金financially supported by the National Key Research and Development Program of China(2022YFC2100800)the National Natural Science Foundation of China(22478296,22078238,52373117,and U23B20121)+1 种基金the Haihe Laboratory of Sustainable Chemical Transformations(24HHWCSS00005)the Open Funding Project of the National Key Laboratory of Biochemical Engineering。
文摘Recent advances in organ transplantation,regenerative medicine,and drug discovery have emphasized the critical importance of effective preservation techniques for organs.Despite these advances,current preservation techniques have significant limitations in maintaining the viability and functional efficacy of organs over the long term.As a result,there is a pressing need to develop reliable and efficient preservation strategies for organs.Currently,the clinical standard for organ preservation involves the use of static cold storage and organ machine perfusion,but these methods can only preserve organs for a couple of days or even a few hours.Notably,the development of cryobiology has yielded promising alternatives.In this review,we aim to provide a comprehensive overview of the progression of organ preservation methods,while emphasizing the limitations of traditional approaches.Moreover,we evaluate advanced preservation techniques for organs,including kidneys,livers,hearts,lungs,and intestines.Furthermore,we share a progress perspective on the future of organ preservation,with the ultimate goal of achieving viable long-term preservation to address the pressing issue of organ shortage.
基金Supported by European Union-Next Generation EU,through the National Recovery and Resilience Plan of the Republic of Bulgaria,No.BG-RRP-2.004-0008.
文摘Fertility preservation and pregnancymanagement are critical considerations for patients undergoing organtransplantation.Innovations in assisted reproductive technologies,hormonalmodulation,and personalized medicine have expanded options for these patients,who face unique challenges due to immunosuppressive therapy and organ functionconcerns.This mini-review explores advancements in cryopreservationtechniques,pre-conception counseling,and multidisciplinary strategies forsafe pregnancies post-transplantation.Emphasis is placed on balancing maternalhealth,graft function,and fetal outcomes.The integration of reproductive andtransplant medicine is paving the way for improved quality of life andreproductive autonomy for this patient population.
基金Supported by the National Natural Science Foundation of China(U22B6004)Project of PetroChina Research Institute of Petroleum Exploration and Development(2022yjcq03)Core Technology Key Project of China Petroleum Changqing Oilfield Company(KJZX2023-01).
文摘In addition to the organic matter type,abundance,thermal maturity,and shale reservoir space,the preservation conditions of source rocks play a key factor in affecting the quantity and quality of retained hydrocarbons in source rocks of lacustrine shale,yet this aspect has received little attention.This paper,based on the case analysis,explores how preservation conditions influence the enrichment of mobile hydrocarbons in shale oil.Research showns that good preservation conditions play three key roles.(1)Ensure the retention of sufficient light hydrocarbons(C_(1)–C_(13)),medium hydrocarbons(C_(14)–C_(25))and small molecular aromatics(including 1–2 benzene rings)in the formation,which enhances the fluidity and flow of shale oil;(2)Maintain a high energy field(abnormally high pressure),thus facilitating the maximum outflow of shale oil;(3)Ensure that the retained hydrocarbons have the miscible flow condition of multi-component hydrocarbons(light hydrocarbons,medium hydrocarbons,heavy hydrocarbons,and heteroatomic compounds),so that the heavy hydrocarbons(C_(25+))and heavy components(non-hydrocarbons and asphaltenes)have improved fluidity and maximum flow capacity.In conclusion,in addition to the advantages of organic matter type,abundance,thermal maturity,and reservoir space,good preservation conditions of shale layers are essential for the formation of economically viable shale oil reservoirs,which should be incorporated into the evaluation criteria of shale oil-rich areas/segments and considered a necessary factor when selecting favorable exploration targets.
文摘In this study,composite films consisting of polylactic acid(PLA),ethyl cellulose(EC),and zein were prepared by solution casting method,and their performance and application in chilled fresh meat preservation were investigated.The results showed that the three materials had satisfactory compatibility in the composite film.Addition of EC and zein effectively improved the mechanical properties,thermodynamic properties,surface hydrophilicity,oxygen permeability,and degradation properties of PLA films.When the ratio of PLA to EC was 3:7,the tensile strength and elongation at break reached maximum values of 16.6 MPa and 30.5%,respectively.Moreover,under different conditions,the composite film exhibited better degradability than the PLA film.The composite film with a 3:7 ratio of PLA to EC had the best performance,with a degradation rate of 21.75%after 84 days.Chilled fresh meat wrapped with the composite film showed significantly improved antioxidant,antibacterial,and water-holding properties.
文摘To alleviate the quality deterioration and extend the shelf life of crucian carp fillets,a bio-based active composite film integrating eugenol(EG)-loaded mesoporous silica nanoparticles(MSNs)modified with caffeic acid(CA)within the zein matrix(EG/CA-MSN/Zein)was developed.This study compared the effects of EG/CA-MSN/Zein,polyethylene(PE),and zein films on the quality of fish fillets during refrigerated storage.The results showed that the EG/CA-MSN/Zein film significantly retarded the increase in pH and microbial growth.Moreover,the film had higher water-holding capacity(WHC),better texture,and color stability,as indicated by low-field nuclear magnetic resonance(LF-NMR),magnetic resonance imaging(MRI),and sensory evaluation.The EG/CA-MSN/Zein composite film extended the shelf life of crucian carp fillets by 3-4 days,highlighting its potential as an environmentally friendly and efficient packaging material for aquatic products.
基金financially supported by Natural Science Foundations of Hebei Province(C2022208003)Youth Top Talent Plan in Hebei Province。
文摘Mold spoilage and nutrition enrichment are major concerns of Chinese steamed bread,and using functional lactic acid bacteria as starter to play the role of in-situ preservation and nutritional fortification was a promising alternative.Hence,Lactiplantibacillus plantarum P10 capable of inhibiting spoilage microorganisms and producing γ-aminobutyric acid(GABA)(380 mg/L)was screened from Chinese pickle.Metabolomics analysis showed that P10 produced key antimicrobial metabolites mainly including ten organic acids,aldehyde,alkaloid.Besides,P10 might exhibit health beneficial effects such as degrading cholesterol,lowering blood glucose and reducing uric acid due to the production of deoxycholic acid and Ile-Pro-Ile,and the degradation of purine.Finally,the promising application potential of P10 was evidenced in steamed bread with extended shelf life,excellent anti-aging effect,and 24.5-fold enrichment of GABA.Conclusively,this investigation could provide scientific basis for the application of P10 as a functional starter in steamed bread.
基金the Ministry of Agriculture and Rural Affairs of Chinathe Department of Science and Technology of the Hainan Province for their support+2 种基金financially supported by the National Key R&D Program of China(No. 2022YFD2301201)Hainan Province Science and Technology Special Fund (No. ZDYF2024XDNY284)Earmarked Fund for China Agriculture Research System (No.CARS-33-JG1)
文摘The thioacetamide derivative(TD)-composite preservation system(TDCPS)exhibits superior preservation effects on natural rubber latex(NRL)and significantly enhances its vulcanization efficiency and mechanical properties.This study primarily investigated the principal chemical groups and mechanism of action of TDCPS in promoting NRL vulcanization through a comparative analysis.The results indicated that the key functional groups(thioamide and pyridine)in TDCPS synergistically accelerated crosslinking,reducing the vulcanization time by 41.18%compared to the high-ammonia(HA)preservation system.At an optimal TDCPS dosage of 5 mmol·L^(−1),vulcanized films achieved a tensile strength of 34.18 MPa,with a sulfur content of 1.5 phr further improving the strength by 42.26%.TD outperformed the conventional accelerators 2-imidazolidinethione(ETU)and 3-hydroxypyridine(3-Hp)in promoting the crosslinking density and mechanical performance while eliminating ammonia-related environmental risks.This eco-friendly system demonstrates the industrial potential for sustainable rubber production.
基金supported by the National Natural Science Foundation of China(32060520)Science and Technology Talents and Platform Program of Yunnan Province(202105AF150049)University Key Laboratory of Food Microbial Resources and Utilization in Yunnan Province(Yunjiaofa[2018]No.135).
文摘In order to ensure food safety,controlling foodborne pathogen contamination is of utmost importance.Growing apprehensions regarding the safety of synthetic antimicrobials,due to their adverse health effects,have prompted a search for alternative options.Plant natural products(PNPs)with antimicrobial activity are being explored as a viable alternative.Among the various antimicrobial natural products studied,plant essential oils,plant flavonoids,plant polyphenols,plant polysaccharides,and plant antimicrobial peptides have been identified as potential candidates.PNPs demonstrate a diverse array of antimicrobial mechanisms,encompassing cell wall and membrane damage,interference with genetic replication,disruption of energy metabolism,and induction of oxidative stress at the single-cell level,as well as inhibition of biofilm formation and quorum sensing at the population level.Certain PNPs have been harnessed as natural antimicrobial agents for the food preservation.The utilization of encapsulation technology proves to be an effective strategy in protecting PNPs,thereby ensuring good antimicrobial efficacy,enhanced dispersibility,and controlled release within food products.The utilization of nanoemulsions,nanoliposomes,edible packaging,electrospun nanofibers,and microcapsules formed by encapsulation has enriched the ways in which PNPs can be applied in food preservation.Although PNPs have great potential in food preservation,their widespread application in the food industry is currently constrained by factors such as production costs,safety concerns,and legal considerations.Chemical synthesis and biosynthesis pathways offer viable strategies for reducing the cost of producing PNPs,and ongoing efforts to assess safety and improve regulatory frameworks are likely to facilitate the broader adoption of PNPs in food preservation practices.This article provides an overview of the main types of PNPs with antimicrobial activity and their properties,focusing on their mechanisms of action.Additionally,it summarizes the use of PNPs in food preservation and discusses the characteristics and applications of different encapsulation technologies.Lastly,the paper briefly analyzes current limitations and proposes potential future trends for this field.
文摘BACKGROUND Low rectal cancer poses a significant surgical challenge because of its close proximity to the anal sphincter,often requiring radical resection with permanent colostomy to achieve oncological safety.Revisited rectal anatomy,advances in surgical techniques and neoadjuvant therapies have enabled the possibility of sphincter-preserving procedures,however,it is uniformly not applicable.Selecting appropriate candidates for sphincter preservation is crucial,as an illadvised approach may compromise oncological outcome or lead to poor functional outcomes.Currently there is no consensus-which clinical,anatomical,or molecular factors most accurately predict the feasibility of sphincter-preserving surgery(SPS)in this subset of patients.By identifying these predictors,the study seeks to support improved patient selection,enhance surgical planning,and ultimately contribute to better functional and oncological outcomes in patients with low rectal cancer.AIM To identify predictive factors that determine the feasibility of SPS in patients with low rectal cancer.METHODS A comprehensive literature search was conducted using PubMed/MEDLINE databases.The search focused on various factors influencing the feasibility of SPS in low rectal cancer.These included patient-related factors,anatomical considerations,findings from different imaging modalities,advancements in diagnostic tools and techniques,and the role of neoadjuvant chemoradiotherapy.The relevance of each factor in predicting the potential for sphincter preservation was critically analyzed and presented based on the current evidence RESULTS Multiple studies have identified a range of predictive factors influencing the feasibility of SPS in low rectal cancer.Patient-related factors include age,sex,preoperative continence status,comorbidities,and body mass index.Anatomical considerations,such as tumor distance from the anal verge,involvement of the external anal sphincter,and levator ani muscles,also play a critical role.Additionally,a favourable response to neoadjuvant chemoradiotherapy has been associated with improved suitability for sphincter preservation.Several biomarkers,such as inflammatory markers like interleukins and C-reactive protein,as well as tumor markers like carcinoembryonic antigen,are important.Molecular markers,including BRAF and KRAS mutations and microsatellite instability status,have been linked to prognosis and may further guide decision-making regarding sphincter-preserving approaches.Artificial intelligence(AI)can further add in to select an ideal patient for sphincter preservation.CONCLUSION SPS is feasible in low rectal cancer and depends on patient factors,tumor anatomy and biology,preoperative treatment response,and biomarkers.In addition,tools and technology including AI can further help in selecting an ideal patient for long term optimal outcome.
基金Supported by The National Natural Science Foundation of China,No.82460107Science and Technology Talent Support Program of Shaanxi Provincial People's Hospital,No.2021 LJ-05.
文摘BACKGROUND The surgical management of rectal cancer is continuously advancing,with a current emphasis on minimising the need for a permanent stoma.Understanding the risk factors influencing sphincter preservation is crucial for guiding clinical decision-making and optimising preoperative patient evaluation.AIM To examine the risk factors influencing sphincter preservation in laparoscopic radical rectal cancer surgery.METHODS A retrospective analysis of the demographics,preoperative and intraoperative data,and pathological findings of 179 patients with rectal cancer who underwent laparoscopic radical rectal cancer surgery at our hospital between January 2022 and December 2023 was conducted.These clinical data were compared between two groups:Patients with sphincter preservation and those without,categorised as the sphincter-preserved and sphincter-unpreserved groups,respectively.RESULTS Of the 179 patients analysed,150 were in the sphincter-preserved group and 29 were in the sphincter-unpreserved group.Tumour height was significantly greater in the sphincter-preserved group compared to the sphincter-unpreserved group.Conversely,elevated levels of carcinoembryonic antigen,carbohydrate antigen 19-9,and plasma D-dimer were significantly higher in the sphincter-unpreserved group.Significant differences were also observed between the two groups in terms of place of residence,presence of colonic polyps,neoadjuvant chemotherapy,preoperative radiotherapy,mucinous adenocarcinoma,nerve invasion,and tumour height.No significant differences were observed for other parameters.Logistic regression analysis identified colonic polyps,mucinous adenocarcinoma,nerve invasion,and tumour height as independent risk factors for sphincter preser-vation.CONCLUSION Several risk factors influencing sphincter preservation in laparoscopic radical rectal cancer surgery were identified.These factors could be valuable tools for guiding clinical decision-making and optimising preoperative patient evaluations.
基金supported by the National Natural Science Foundation of China(Nos.42162013,42002095)the Foundation of State Key Laboratory of Nuclear Resources and Environment(Nos.2022NRE34,NRE2021-01)+1 种基金Jiangxi Provincial Natural Science Foundation(Nos.20242BAB26048,20242BAB25178)Fund of National Key Laboratory of Science and Technology on Remote Sensing Information and imagery Analysis,Beijing Research Institute of Uranium Geology(No.6142A01210405)。
文摘The Yangchuling porphyry W-Mo deposit(YPWD),located in the Jiangnan porphyryskarn tungsten ore belt,is one of the most important and large-scale porphyry W-Mo deposits in South China.While previous zircon U-Pb and molybdenite Re-Os data suggest that Yangchuling WMo ore bodies formed almost simultaneously with granodiorite and monzogranitic porphyry at~150–144 Ma,their post emplacement history remains poorly understood,making their preservation status at depth uncertain.In this paper,new zircon and apatite(U-Th)/He and apatite fission track(ZHe,AHe and AFT,respectively)data of one hornfels and five intrusive rocks from a 1000-meter borehole are presented.These,together with new inverse thermal history models and previous geochronological data,help elucidate the post-diagenetic exhumation history and preservation status of the Yangchuling porphyry W-Mo deposit.In general,ZHe and AHe ages decrease gradually from the near surface downwards and have relatively little intra-sample variation,ranging from 133 to 73Ma and 67 to 25 Ma,respectively.All four granodiorites yield similar AFT ages that range from 63 to 55 Ma with mean track lengths varying from 12.2±0.7 to 12.6±0.5μm.Thermal history modelling indicates that the Yangchuling ore district experienced slow,monotonic cooling since the Cretaceous.Age-depth relationships are interpreted as recording~3.7±0.8 km of Cretaceous-recent exhumation in response to regional extension throughout South China thought to have been driven by subduction retreat of the Paleo-Pacific Plate.Comparison of estimated net exhumation and previous metallogenic depth of~4–5 km suggests that W-Mo ore bodies could still exist at depths of up to~1.3±0.8 km relative to Earth surface in the YPWD region.Preservation of the YPWD is attributed to the limited amount of regional denudation during the Late Cretaceous and Cenozoic.
基金funded by the National Natural Science Foundation of China(No.81971759 and No.82171604)the Guangdong Basic and Applied Basic Research Foundation(2023B1515020108)+3 种基金the Science and Technology Program of Guangzhou(202206010089)the Excellent Talents Training Project of The Sixth Affiliated Hospital of Sun Yat-sen University(R20210217202601970)the Postdoctoral Fellowship Program of CPSF(GZC20233216)the Basic and Applied Basic Research Foundation of Guangdong Province(2021A1515111195).
文摘The aim of this investigation was to determine the optimal storage medium for testicular hypothermic transportation and identify the ideal concentration for the application of the protective agent 5-aminolevulinic acid(5-ALA).Furthermore,this study aimed to explore the underlying mechanism of the protective effects of 5-ALA.First,we collected and stored mouse testicular fragments in different media,including Hank’s balanced salt solution(HBSS;n=5),Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12(DMEM/F12;n=5),and alpha-minimum essential medium(αMEM;n=5).Storage of testicular tissue in HBSS preserved the integrity of testicular morphology better than that in the DMEM/F12 group(P<0.05)and theαMEM group(P<0.01).Testicular fragments were subsequently placed in HBSS with various concentrations of 5-ALA(0[control],1 mmol l−1,2 mmol l−1,and 5 mmol l−1)to determine the most effective concentration of 5-ALA.The 2 mmol l−15-ALA group(n=3)presented the highest positive rate of spermatogonial stem cells compared with those in the control,1 mmol l−1,and 5 mmol l−15-ALA groups.Finally,the tissue fragments were preserved in HBSS with control(n=3)and 2 mmol l−15-ALA(n=3)under low-temperature conditions.A comparative analysis was performed against fresh testes(n=3)to elucidate the underlying mechanism of 5-ALA.Gene set enrichment analysis(GSEA)for WikiPathways revealed that the p38 mitogen-activated protein kinase(MAPK)signaling pathway was downregulated in the 2 mmol l−15-ALA group compared with that in the control group(normalized enrichment score[NES]=−1.57,false discovery rate[FDR]=0.229,and P=0.019).In conclusion,these data suggest that using 2 mmol l−15-ALA in HBSS effectively protected the viability of spermatogonial stem cells upon hypothermic transportation.
基金supported by the National Natural Science Foundation of China(Nos.42488101,42141021,42172149,42172168,U2244209)the Tencent Foundation through the XPLORER PRIZE。
文摘Occurrence and abundance of molecular hydrogen in natural geologic reservoirs are enigmatic,due to its various sources,diverse migration pathways and complicated biological and chemical reactions.Natural gas samples containing hydrogen from producing wells in several sedimentary basins in China were collected in this study,and gas abundances and isotopic compositions of these gases were compared with those in global petroliferous basins and deep intrusive rocks.Several geochemical indicators were suggested for identifying sources,migration and accumulation mechanisms of hydrogen in the subsurface environment.Hydrogen contents in natural gas deposits have contributions from various sources with the following high-to-low order:microbial degradation>serpentinization>deep mantle volatile release>radiation-induced water decomposition>thermal cracking of organic matter.A hydrogen-rich reservoir in Kansas,USA,is specifically analyzed to determine its formation mechanism.This study suggests that future exploration of geological hydrogen resources may focus on the igneous rock bodies with overlying dense sedimentary rocks in the continental rift systems.
基金Supported by Zhejiang Medical and Health Science and Technology Project,No.2024KY133Zhejiang Provincial Plan for Traditional Chinese Medicine Science and Technology Project,No.2025ZR111 and No.2023ZL038.
文摘BACKGROUND Osteonecrosis of the femoral head(ONFH)is a prevalent clinical condition,and as the affected population becomes younger and more widespread,an increasing number of patients prefer to retain their own hip joints.In comparison to joint replacement and hip-preserving surgeries,conservative hip preservation treatment is gaining more recognition from both clinicians and patients.AIM To observe the clinical efficacy and influencing factors of conservative hip preservation treatment for patients with ONFH.METHODS A total of 73 patients(119 hips)were included,and patients were grouped based on Association Research Circulation Osseous(ARCO)stage,Japanese Investigation Committee(JIC)classification,lesion size,and collapse degree.The clinical efficacy of conservative hip preservation treatment was evaluated from two aspects:Radiological progression and clinical functional scores[Harris Hip Score,Western Ontario and McMaster Universities Arthritis Index(WOMAC),international Hip Outcome Tool 12(iHOT-12),and visual analogue scale(VAS)].Analyzing the success rate of conservative hip preservation based on conversion to total hip arthroplasty or hip preservation surgery.RESULTS In this retrospective cohort study,we retrospectively analyzed 73 patients(119 hips)with an average follow-up time of 53.58±26.80(18-129)months.The overall success rate of hip preservation is 90.76%.The success rates of hip preservation in ARCO I-II,IIIa,and IIIb were 97.67%,91.53%,and 70.59%,respectively(P<0.05).The success rates of hip preservation for JIC A-B,C1,and C2 were 93.37%,92.59%,and 77.78%,respectively(P<0.05).The success rates of hip preservation with collapse degree≤2 mm and>2 mm were 94.12%and 70.59%,respectively(P<0.05).There was no significant difference in the success rate of hip preservation between different lesion sizes.In terms of clinical function,the Harris Hip Score,WOMAC,iHOT-12,and VAS scores at the last follow-up were significantly better than before treatment(P<0.05).The VAS scores of ARCO IIIa and IIIb were significantly worse than those of ARCO I and II(P<0.05).The iHOT-12,WOMAC,and VAS scores were significantly better with the lesion size<15%(P<0.05).In terms of radiological results,there was significant progress in ARCO staging and collapse degree after conservative hip preservation treatment,but there was no significant difference in JIC classification and lesion size.CONCLUSION Conservative hip preservation treatment can effectively treat ONFH,prevent the progression of the necrosis,and significantly improve the clinical function of patients.The radiological staging and lesion size at the initial visit may help determine the prognosis of conservative hip preservation treatment.
文摘Porous organic polymers are remarkably versatile materials with porous and carefully designed structures.They complement traditional preservation methods by overcoming their limitations and significantly extending the shelf life of preserved products.Notably,porous hollow nanospheres(PHNs),with their unique hollow structures capable of adsorbing and releasing organic molecules,have garnered considerable attention in food preservation.However,most PHNs are challenging to synthesize in one step,and PHNs are usually in powder form,which makes it challenging to apply them directly.In this study,we successfully synthesized PHNs in one step using the Friedel–Crafts reaction.The PHNs,adsorbed with hexanal molecules,were then embedded in polymer fibers to create composites via electrospinning.The preservation effect of the composite nanofiber membranes was investigated by determining the changes in appearance,weight,peel hardness,and pulp sugar content of three fruits,namely strawberries,bananas,and kumquats,after several days of storage.In comparison to pure poly(ε-caprolactone)fiber membranes,PHNs containing hexanal molecules slowed down the oxidative deterioration process and enhanced the quality and flavor of preserved fruits.This research presents innovative approaches for using porous organic polymers in food preservation and serves as a valuable reference for the development of future food packaging materials.
基金Supported by the National Natural Science Foundation of China(U23B20154)General Program of the National Natural Science Foundation of China(42372169)。
文摘In recent years,drilling data from wells Pengshen 10,Heshen 9,Tongshen 17 and Zhengyang 1 in the Sichuan Basin have confirmed the presence of a set of porous reef-beach limestone reservoirs in the Upper Permian Changxing Formation,which breaks the traditional view that deep carbonate oil and gas are only distributed in porous dolomite reservoirs and karst fracture-cavity limestone reservoirs.Through core and thin section observations,geochemical analysis,and well-seismic based reservoir identification and tracking,the study on formation mechanism of pores in deep reef-beach limestone reservoirs is carried out,this study provides insights in four aspects.(1)Porous reef-beach limestone reservoirs are developed in the Changxing Formation in deep-buried layers.The reservoir space is composed of intergranular pores,framework pores,intra-fossil pores,moldic pores and dissolution pores,which are formed in depositional and epigenetic environments.(2)The intermittently distributed porous reef-beach complexes are surrounded by relatively dense micrite limestone,which leads to the formation of local abnormal high-pressure inside the reef-beach complexes with the temperature increased.(3)The floor of the Changxing Formation reservoir is composed with interbedded tight mudstone and limestone of the Upper Permian Wujiaping Formation,and the roof is the tight micrite limestone interbedded with mudstone of the first member of Lower Triassic Feixianguan Formation.Under the clamping of dense roof and floor,the abnormal high-pressure in the Changxing Formation is formed.Abnormal high-pressure(overpressured compartment)is the key to maintain the pores formed in the depositional and epigenetic environments in deep-buried layers.(4)Based on the identification of roof,floor and reef-beach complexes,the favorable reef-beach limestone reservoir distribution area of 10.3×10^(4) km^(2) is predicted by well-seismic integration.These insights lay the theoretical foundation for the development of deep porous limestone reservoirs,expand the new field of exploration of deep-buried limestone reservoirs in the Sichuan Basin.
基金funded by the Academic Leadership Grant of Universitas Padjadjaran,Bandung,Indonesia,with grant number 1540/UN6.3.1/PT.00/2024the Research Collaboration Center for Biomass and Biorefinery,Bandung,Indonesia,with grant number B-1723/II.7/HK.01.00/4/2024.
文摘Konjac is an ideal candidate for edible coatings on fruits due to its hydrophilic properties,film-forming ability,barrier properties,safety,and biodegradability.Meanwhile,the high market demand for strawberries necessitates post-harvest treatment to extend their shelf life and preserve their quality,as strawberries are known for their fragile skin and soft texture.To fully utilize konjac and develop high-quality coatingfilms,native konjacflour(NKF)and konjac glucomannan(KGM)were extracted from its corm and used as a coatingfilm for strawberries in the present study.Therefore,this study aimed to compare the physical properties of thefilm coatings between NKF and KGM,and evaluate their effects on strawberries preservation over 7 days of storage.A multistage extrac-tion process was employed to isolate NKF and KGM,after which the glucomannan content was measured.NKF yield was 31.81%,exceeding KGM yield of 26.42%,and the glucomannan content obtained of NKF(25.93%)was higher than KGM(21.41%).Nuclear magnetic resonance spectroscopy confirmed that both NKF and KGM con-tain glucomannan in their structure.Furthermore,both NKF and KGM were combined with carboxymethyl cel-lulose(CMC)and glycerol to produce eight thin-layerfilms to assess their physical and mechanical properties.Compared to the KGM variant,the NKF variant generally exhibited higher moisture content,water vapor trans-mission rate,and tensile strength.However,NKF was less effective than KGM in extending strawberry storage life,leading to faster color changes and greater weight loss,despite maintaining similar hardness values.Nonetheless,konjac-based coatings were generally effective at maintaining the freshness and quality of strawberries compared to uncoated samples.Konjac shows promise as an edible coating,improving fresh produce shelf life and appeal,aligning with consumer preferences for natural and sustainable products.
文摘In 2004 and 2014,two monographs on hypobaric storage(LP)were published by Stanley P.Burg.Based on his theoretical framework and technological advancements,as well as the research and development of equipment conducted under his guidance,alongside customer practices and reflections on various scientific literature both domestically and internationally,it is posited that,as articulated in the monograph,misconceptions regarding his theory and technology in Western scientific literature prior to 1985 continue to persist.This ongoing dissemination of misunderstandings has resulted in a near stagnation of research and has adversely impacted the Chinese academic community as well.Consequently,it is essential to delineate the characteristics and main points of its theory and technology,with the aim of offering guidance to individuals seeking to comprehend its foundational purpose.LP technology is a dynamic physical technology that continuously and uninterruptedly extracts air from a closed container and simultaneously introduces fresh,low-pressure moist air from the external environment,while maintaining specific levels of humidity and/or temperature within the container and upholding a predetermined pressure value.Preservation technology is the collective term for the set of various technical parameters associated with preservation,including pressure,relative humidity,and other relevant factors,to which LP equipment is specifically designed.The theory of LP is characterized by the enhanced diffusion of gases and vapors that enter and exit the commodity in a dynamic manner under low pressure conditions.The theoretical points involve equipment performance,low pressure,the impact of trace concentrations of gases such as O_(2),CO_(2) and C_(2)H_(4) that naturally occur at low pressure,diffusive mass transfer,heat transfer,and impacts on the activity of enzymes associated with maturation and senescence.The technology is characterized by dynamic low pressure,and the range of commodities preserved is comparable to that of refrigeration.However,certain commodities exiting the hypobaric environment possess subsequent preservation advantages that are not available through refrigeration.The main points of the technology encompass an extended sotrage life,a postponement of quality degradation,minimized water loss,the suppression of pathogen growth,and the killing of both internal and external insects of the commodity under dynamic low pressure conditions.The core advantage of LP technology lies in its ability to significantly reduce water loss,inhibit respiration and C_(2)H_(4) action,and pathogen growth,killing insects and modulate the activity of enzymes associated with maturation and senescence in post-harvest fresh horticultural products.Consequently,this technology plays a crucial role in prolonging the post-harvest lifespan of these commodities and mitigating quality degradation.Over the past decade,researchers in China have developed a hypobaric short period treatment technology,grounded in LP theory and technical practice,which is commonly referred to as hypobaric treatment.This method has garnered significant attention,leading to an increase in both domestic and international research.A growing body of literature categorizes LP as hypobaric treatment,while some studies also consider vacuum packaging and modified atmosphere packaging(MAP)as LP or hypobaric treatment.Misunderstandings are exacerbated by confusion surrounding nomenclature,which,in conjunction with pre-existing misconceptions,represents a significant barrier to both the research and practical application of the technology.The successful commercial implementation of a vacuum cold fresh chain,centered on LP or hypobaric treatment technology,may be the sole solution to the prevailing misunderstandings associated with LP.
基金National Natural Science Foundation of China,Grant/Award Number:42230812。
文摘The 10000-m ultradeep dolomite reservoir holds significant potential as a successor field for future oil and gas exploration in China's marine craton basin.However,major challenges such as the genesis of dolomite,the formation time of high-quality reservoirs,and the preservation mechanism of reservoirs have always limited exploration decision-making.This research systematically elaborates on the genesis and reservoir-forming mechanisms of Sinian-Cambrian dolomite,discussing the ancient marine environment where microorganisms and dolomite develop,which controls the formation of large-scale Precambrian-Cambrian dolomite.The periodic changes inMg isotopes and sedimentary cycles show that the thick-layered dolomite is the result of different dolomitization processes superimposed on a spatiotemporal scale.Lattice defects and dolomite embryos can promote dolomitization.By simulating the dissolution of typical calcite and dolomite crystal faces in different solution systems and calculating their molecular weights,the essence of heterogeneous dissolution and pore formation on typical calcite and dolomite crystal faces was revealed,and the mechanism of dolomitization was also demonstrated.The properties of calcite and dolomite(104)/(110)grain boundaries and their dissolution mechanism in carbonate solution were revealed,showing the limiting factors of the dolomitization process and the preservation mechanism of deep buried dolomite reservoirs.The in situ laser U-Pb isotope dating technique has demonstrated the timing of dolomitization and pore formation in ancient carbonate rocks.This research also proposed that dolomitization occurred during the quasi-contemporaneous or shallow-burial periods within 50Ma after deposition and pores formed during the quasi-contemporaneous to the early diagenetic periods.And it was clear that the quasi-contemporaneous dolomitization was the key period for reservoir formation.The systematic characterization of the spatial distribution of the deepest dolomite reservoirs in multiple sets of the Sinian and the Cambrian in the Chinese craton basins provides an important basis for the distribution prediction of large-scale dolomite reservoirs.It clarifies the targets for oil and gas exploration at depths over 10000 m.The research on dolomite in this study will greatly promote China's ultradeep oil and gas exploration and lead the Chinese petroleum industry into a new era of 10000-m deep oil exploration.
基金supported by the Henan Provincial Traditional Chinese Medicine Culture and Management Research Project(TCM2022036)Henan Provincial Traditional Chinese Medicine Culture Publication Funding Special Project(TCMCB2023016)+3 种基金Henan University of Chinese Medicine Research Seedling Project(MP2023-29)National Administration of Traditional Chinese Medicine Science and Technology Project(GZY-KJS-2022-043-1)Henan Culture-Promoting Project Cultural Research Special Project(2022XWH231)National Administration of Traditional Chinese Medicine Science and Technology Project(GZY-KJS-2022-037).
文摘By analyzing the structure and academic characteristics of early Western Han medical texts such as Prescriptions for Fifty-Two Diseases(Wu Shi Er Bing Fang)and Prescriptions for Health Preservation(Yang Sheng Fang),and considering the development of philosophical thinking during the pre-Qin and Han periods,this study summarizes how Zhongjing Zhang’s Treatise on Cold Damage and Miscellaneous Diseases(Shang Han Za Bing Lun)inherited pre-Qin and Han philosophical thought while further integrating Daoist“natural law”cosmology,Confucian“benevolence”social ethics,and the“isomorphic unity of heaven and humanity”medical theory in Yellow Emperor’s Inner Classic(Huang Di Nei Jing).It proposes health preservation concepts encompassing heaven-human,body-spirit,and benevolence perspectives,along with dietary hygiene,representing the medical embodiment of pre-Han“virtue cultivation”and“body nurturing”life philosophies that profoundly influenced subsequent traditional Chinese medicine health preservation theory and practice.
