Increased soil erosion as a consequence of karst rocky desertification(KRD)reduces soil quality and plant diversity.Premna puberula Pamp.is a perennial understory shrub that can adapt to the harsh environment of KRD a...Increased soil erosion as a consequence of karst rocky desertification(KRD)reduces soil quality and plant diversity.Premna puberula Pamp.is a perennial understory shrub that can adapt to the harsh environment of KRD and has potential value for KRD environmental restoration.We measure morphological and photosynthetic characteristics,nutrient accumulation,and soil physical and chemical properties of two-year-old P.puberula planted in KRD habitat and non-rocky desertification areas,and investigate how it adapts to this habitat.In KRD areas,P.puberula reduces aboveground water loss by decreasing leaf area,stomatal conductance,and transpiration rate,while increasing leaf thickness,root length and number,and increases belowground biomass to absorb more water and nutrients.In KRD areas,drought leads to decreased leaf water content,stomatal conductance,intercellular CO_(2) concentration,and chlorophyll content,and decreased photosynthetic rate.Soluble sugar and protein contents increase in KRD areas in response to water stress,but pectin and flavonoid contents decrease.Correlation analysis reveals that leaf area and thickness are important functional traits that affect leaf water content and photosynthesis.Totals of leaf carbon,nitrogen,and phosphorus,along with C:N,C:P,and N:P ratios,are coupled with leaf photosynthetic characteristics and root development.Redundancy analysis reveals that available K and P,and total P,K,and N in soil mainly influence the differences in plant functional traits,explaining 95.8%,95.5%,91.1%,85.8%,and 74%of variation,respectively.We hypothesize that P.puberula reduces aboveground productivity to maintain its water balance,and acquires more water and nutrients by increasing belowground biomass.It indicates that this species adapts to arid KRD environments.展开更多
为了研究狐臭柴叶水提取液(Premna puberula Pamp.leaves water extract,PPW)对秀丽隐杆线虫的抗衰老作用,首先以秀丽隐杆线虫作为衰老模型,测定狐臭柴叶水提取液对秀丽隐杆线虫自然寿命、虫体长度、运动能力、紫外应激能力及丙二醛含...为了研究狐臭柴叶水提取液(Premna puberula Pamp.leaves water extract,PPW)对秀丽隐杆线虫的抗衰老作用,首先以秀丽隐杆线虫作为衰老模型,测定狐臭柴叶水提取液对秀丽隐杆线虫自然寿命、虫体长度、运动能力、紫外应激能力及丙二醛含量的影响,再以苯酚-硫酸法、酒石酸铁法、硝酸钠-硝酸铝法及考马斯亮蓝法分别测定PPW中多糖、总黄酮、总酚、蛋白质的含量。实验结果表明,与空白对照相比,在PPW含量为25%、50%、75%时,分别延长了秀丽隐杆线虫自然寿命的13.83%、16.42%、12.27%,在紫外应激条件下分别延长线虫寿命的21.12%、16.81%、32.96%。在PPW含量为50%和75%时,线虫的运动能力、虫体长度显著高于空白组(P<0.05),显著降低了线虫体内丙二醛的含量(P<0.05)。PPW中多糖、总黄酮、总酚、蛋白质含量分别为54.30±3.39、13.52±0.90、15.52±0.53、13.87±0.30 mg/g。结果说明,狐臭柴叶水提取液可以延缓秀丽隐杆线虫的衰老、降低体内丙二醛含量、促进线虫生长发育、提高运动能力和抗紫外应激能力。此结论可为狐臭柴叶进一步的研究与开发利用提供参考,尤其是为探究狐臭柴的食用价值、药用价值和医疗保健功能奠定理论基础。展开更多
基金supported by the Key Core Technology Research Project for Mountainous Agriculture in Guizhou(GZNYGJHX-2023011)the Science and Technology Support Plan Project of Guizhou(2018-2797)+2 种基金the Major Special Project of Science and Technology Program in Guizhou(2017-5411-06)the Construction Project of State Engineering Technology Institute for Karst Desertification Control of China(2012FU125X13)the Construction Project of Modern Industry Technology system of traditional Chinese Medicinal Materials in Guizhou(GZCYTX-02).
文摘Increased soil erosion as a consequence of karst rocky desertification(KRD)reduces soil quality and plant diversity.Premna puberula Pamp.is a perennial understory shrub that can adapt to the harsh environment of KRD and has potential value for KRD environmental restoration.We measure morphological and photosynthetic characteristics,nutrient accumulation,and soil physical and chemical properties of two-year-old P.puberula planted in KRD habitat and non-rocky desertification areas,and investigate how it adapts to this habitat.In KRD areas,P.puberula reduces aboveground water loss by decreasing leaf area,stomatal conductance,and transpiration rate,while increasing leaf thickness,root length and number,and increases belowground biomass to absorb more water and nutrients.In KRD areas,drought leads to decreased leaf water content,stomatal conductance,intercellular CO_(2) concentration,and chlorophyll content,and decreased photosynthetic rate.Soluble sugar and protein contents increase in KRD areas in response to water stress,but pectin and flavonoid contents decrease.Correlation analysis reveals that leaf area and thickness are important functional traits that affect leaf water content and photosynthesis.Totals of leaf carbon,nitrogen,and phosphorus,along with C:N,C:P,and N:P ratios,are coupled with leaf photosynthetic characteristics and root development.Redundancy analysis reveals that available K and P,and total P,K,and N in soil mainly influence the differences in plant functional traits,explaining 95.8%,95.5%,91.1%,85.8%,and 74%of variation,respectively.We hypothesize that P.puberula reduces aboveground productivity to maintain its water balance,and acquires more water and nutrients by increasing belowground biomass.It indicates that this species adapts to arid KRD environments.
