Dear Editor,We recently reviewed two important studies that investigate the use of botulinum-A toxin(BoNT-A)injections into the bulbospongiosus muscle as a treatment for lifelong premature ejaculation(PE).While both s...Dear Editor,We recently reviewed two important studies that investigate the use of botulinum-A toxin(BoNT-A)injections into the bulbospongiosus muscle as a treatment for lifelong premature ejaculation(PE).While both studies share the goal of evaluating the efficacy and safety of BoNT-A in this context,they reached very different conclusions.The study by Shaher et al.demonstrated significant improvements in ejaculatory latency,indicating that BoNT-A injections may be a helpful treatment for PE.展开更多
This study aimed to compare the complications of preterm twins versus singletons and analyze differences across gestational ages.Preterm twins delivered between 2 March 2022 and 6 November 2022 were compared to an age...This study aimed to compare the complications of preterm twins versus singletons and analyze differences across gestational ages.Preterm twins delivered between 2 March 2022 and 6 November 2022 were compared to an age-matched control group of singletons,involving 65 twins and 103 singletons.The most common complication in premature infants was neonatal jaundice(87.72%),followed by patent foramen ovale(79.76%)and neonatal respiratory distress syndrome(NRDS)(57.14%).Twins had significantly higher Apgar scores at 1,5,and 10 minutes compared to singletons.However,twins showed a higher incidence of ventricular septal defect(VSD)(7.69%)than singletons,with a statistically significant difference.In contrast,twins exhibited significantly lower rates of neonatal jaundice(78.46%),electrolyte imbalance(18.4%),and acid-base imbalance(9.23%)compared to singletons.Furthermore,as gestational age increased,the incidence of intrauterine infection,electrolyte and acid-base imbalances,neonatal coagulation disorders,patent ductus arteriosus(PDA),anemia,and NRDS in preterm infants gradually decreased,with all differences reaching statistical significance(P<0.05).These findings highlight the importance of close monitoring and timely management of complications in premature infants to prevent severe outcomes.展开更多
Background: Prematurely-born individuals tend to exhibit higher resting oxidative stress, although evidence suggests they may be more resistant to acute hypoxia-induced redox balance alterations. We aimed to investiga...Background: Prematurely-born individuals tend to exhibit higher resting oxidative stress, although evidence suggests they may be more resistant to acute hypoxia-induced redox balance alterations. We aimed to investigate the redox balance changes across a 3-day hypobaric hypoxic exposure at 3375 m in healthy adults born preterm(gestational age ≤ 32 weeks) and their term-born(gestational age ≥ 38 weeks)counterparts.Methods: Resting venous blood was obtained in normoxia(prior to altitude exposure), immediately upon arrival to altitude, and the following 3mornings. Antioxidant(superoxide dismutase(SOD), catalase, glutathione peroxidase(GPx), and ferric reducing antioxidant power(FRAP)),pro-oxidant(xanthine oxidase(XO) and myeloperoxidase(MPO)) enzyme activity, oxidative stress markers(advanced oxidation protein product(AOPP) and malondialdehyde(MDA)), nitric oxide(NO) metabolites(nitrites, nitrates, and total nitrite and nitrate(NOx)), and nitrotyrosine were measured in plasma.Results: SOD increased only in the preterm group(p < 0.05). Catalase increased at arrival in preterm group(p < 0.05). XO activity increased at Day 3 for the preterm group, while it increased acutely(arrival and Day 1) in control group. MPO increased in both groups throughout the3 days(p < 0.05). AOPP only increased at arrival in the preterm(p < 0.05) whereas it decreased at arrival up to Day 3(p < 0.05) for control.MDA decreased in control group from arrival onward. Nitrotyrosine decreased in both groups(p < 0.05). Nitrites increased on Day 3(p < 0.05)in control group and decreased on Day 1(p < 0.05) in preterm group.Conclusion: These data indicate that antioxidant enzymes seem to increase immediately upon hypoxic exposure in preterm adults. Conversely, the blunted pro-oxidant enzyme response to prolonged hypoxia exposure suggests that these enzymes may be less sensitive in preterm individuals.These findings lend further support to the potential hypoxic preconditioning effect of preterm birth.展开更多
BACKGROUND Premature infant formula is based on milk and contains energy,vitamins,etc.Breast milk is rich in minerals,such as phosphorus and calcium,and proteins.Both can be used for nutritional support in preterm inf...BACKGROUND Premature infant formula is based on milk and contains energy,vitamins,etc.Breast milk is rich in minerals,such as phosphorus and calcium,and proteins.Both can be used for nutritional support in preterm infants with low-birth-weight.However,their effects on the difference in infant growth rate and postpartum depression are still unclear.AIM To explore the effect of early micro-breastfeeding on the growth rate of preterm infants with low-birth-weight and maternal postpartum depression.METHODS Data of 68 preterm infants with low-birth-weight and their mothers admitted to the Department of Neonatology,Suzhou Ninth Hospital Affiliated to Soochow University,between January 2022 and December 2024,were retrospectively collected.The infants were divided into two groups according to the different enteral feeding methods in the early stage:Control group(n=32 cases,premature infant formula feeding)and observation group(n=36 cases,micro-breastfeeding,i.e.,exclusive breastfeeding,no mixed feeding).The baseline data;feeding status;gastrointestinal adverse reactions such as vomiting,gastric retention,and abdominal distension;growth rate(body weight,body length,and head circumference);and adverse events(infection,cholestasis,hyperbilirubinemia,and necrotizing enterocolitis)were compared between the two groups.Moreover,the maternal postpartum depression status of the two groups based of infants based on the Edinburgh postpartum depression scale(EPDS)was compared.RESULTS No significant difference in baseline data was found between the two groups(P>0.05).The onset time of weight gain(6.49±0.53 days vs 7.09±0.61 days,P<0.001),time for complete meconium excretion(6.28±0.92 days vs 8.31±1.17 days,P<0.001),time for recovery to birth weight(8.81±1.40 days vs 10.95±1.64 days,P<0.001),and time to reach full enteral feeding(12.29±2.08 days vs 15.48±2.27 days,P<0.001)were compared between the observation and control groups.The incidence of vomiting,abdominal distension,and gastric retention was significantly lower in the observation group than in the control group,and the difference was significant(P<0.05).The rates of the weight growth(15.88±1.57 g/day vs 14.84±1.51 g/day,P=0.007),head circumference(0.63±0.08 cm/week vs 0.59±0.05 cm/week,P=0.018),and length(0.80±0.12 cm/week vs 0.73±0.14 cm/week,P=0.029)were compared between the observation and control groups.On hospital admission of preterm infants with low-birthweight,the mothers of the two groups did not show a significant difference in the EPDS scores for postpartum depression(P>0.05).Preterm infants with low-birth-weight were hospitalized for 2 weeks,and the EPDS score for maternal postpartum depression was significantly lower in the observation group than in the control group(8.38±1.47 vs 9.49±2.35,P=0.021).CONCLUSION Compared with preterm infant formula feeding,early micro-breastfeeding can more effectively promote the growth rate of preterm infants with low-birth-weight and reduce the gastrointestinal feeding intolerance and related complications,thereby alleviating mothers’concerns about their children and reducing the risk of postpartum depression.展开更多
Objective:To explore the pharmacological effects and molecular mechanisms of a Jiao'ai decoction(JAD)in treating premature ovarian failure.Specifically,we evaluated the receptor for advanced glycation end(RAGE)pro...Objective:To explore the pharmacological effects and molecular mechanisms of a Jiao'ai decoction(JAD)in treating premature ovarian failure.Specifically,we evaluated the receptor for advanced glycation end(RAGE)products pathway,metabolic disorders,and intestinal flora dysbiosis.Methods:Forty female rats with normal estrous cycles were randomly divided into five groups:control,model,estradiol,low-dose JAD,and high-dose JAD,with 8 rats in each.Except for the control group,the rats in other groups were injected intraperitoneally with cisplatin for 8 days(1.5 mg/kg)to establish a premature ovarian failure model.Starting on the fifth day of cisplatin injections,the estradiol,low-dose JAD,and high-dose JAD groups were administered corresponding drugs for 21 days.Sex hormone levels and pathological changes in the ovaries were measured.Key proteins in the RAGE pathway related to apoptosis,aging,and inflammation,were tested using Western blot.A 16S rRNA analysis of feces and non-targeted metabolism in serum was performed to determine the effects of JAD on intestinal flora and metabolism.Results:Body weight,ovarian index,and the number of follicles at all levels increased in the JAD group.Regarding serum hormones,estradiol,anti-mullerian hormone(AMH)and P levels increased,whereas follicle-stimulating hormone(FSH)and luteinizing hormone(LH)levels decreased in the JAD group.The levels of phosphorylated Akt protein(P-Akt),receptor for advanced glycation end products(RAGE),tumor protein p53(P53),C-reactive protein(CRP),apoptosis regulator BAX(BAX)and Caspase3 were downregulated by JAD,whereas B cell leukemia/lymphoma 2(Bcl-2)and Endothelial nitric oxidase synthase(eNOS)were upregulated.JAD was also found to play an important role in the regulation of metabolic disorders and intestinal ecological imbalances by adjusting species composition and diversity.Conclusion:JAD can protect ovaries by exerting anti-inflammatory and anti-apoptotic effects via inhibition of the RAGE pathway.JAD can also regulate metabolic disorders and maintain the dynamic balance of intestinal flora,thereby contributing to the improvement of the ovarian reserve function.展开更多
Premature senescence in Bacillus thuringiensis(Bt)cotton has emerged as a significant challenge to the formation and realization of fiber yield and quality since its commercialization in 1997.Initially,premature senes...Premature senescence in Bacillus thuringiensis(Bt)cotton has emerged as a significant challenge to the formation and realization of fiber yield and quality since its commercialization in 1997.Initially,premature senescence was thought to be an inherent trait associated with the Bt gene.However,subsequent research and practice have demonstrated that it is not directly linked to the Bt gene but rather results from a physiological imbalance between the sink and source,as well as between the root and shoot in Bt cotton.This short review provides an overview of the causes,mechanisms,and control measures for premature senescence in Bt cotton.It offers valuable insights for future research and the sustainable application of transgenic crops.展开更多
BACKGROUND Disorders of gut-brain interaction(DGBI)are defined as a variable combination of chronic or recurrent gastrointestinal symptoms.Early-life stressors have been implicated as possible contributing factors.AIM...BACKGROUND Disorders of gut-brain interaction(DGBI)are defined as a variable combination of chronic or recurrent gastrointestinal symptoms.Early-life stressors have been implicated as possible contributing factors.AIM To determine if prematurity and neonatal factors influence the development of DGBI in adults.METHODS A case-control study was carried out at a tertiary referral center from July 2019 to July 2021.Cases(adults born with extremely premature<29 weeks of gestation)were recruited from the Health of Adults Born Preterm Investigation cohort.Control subjects were recruited from the general population.All participants completed the Rome IV diagnostic questionnaire online.Cases completed anxiety and depression questionnaires(Patient-Reported Outcomes Measurement Infor-mation System-29 items,Generalized Anxiety Disorder-7 items,Patient Health Questionnaire-9 items).Neonatal data and sociodemographic status were collected.RESULTS A total of 79 cases and 124 controls were enrolled in the study.The group of adults born preterm exhibited a significantly higher prevalence of functional bowel disorders(P=0.01)and a trend suggesting a higher prevalence of func-tional gastroduodenal disorders(P=0.06).Among women born prematurely,the prevalence of functional gastroduodenal disorders,functional bowel disorders,and functional constipation was significantly higher compared to the female control group(P=0.02 for all).The identified risk factors are categorized as directly linked to prematurity(e.g.,chorioamnionitis),indirectly related to prematurity(e.g.,anxiety,depression,and social skills as consequences of prematurity),or independent of prematurity(e.g.,female sex).CONCLUSION This is the first case-control study reporting the prevalence of DGBI in a cohort of well-characterized adults born prematurely.We confirm that prematurity is a risk factor for developing a DGBI.展开更多
The One Health concept acknowledges the importance of multiple dimensions in controlling antimicrobial resistance(AMR).However,our understanding of how anthropological,socioeconomic,and environmental factors drive AMR...The One Health concept acknowledges the importance of multiple dimensions in controlling antimicrobial resistance(AMR).However,our understanding of how anthropological,socioeconomic,and environmental factors drive AMR at a national level remains limited.To explore associations between potential contributing factors and AMR,this study analyzed an extensive database comprising 13 major antibioticresistant bacteria and over 30 predictors(e.g.,air pollution,antibiotic usage,economy,husbandry,public services,health services,education,diet,climate,and population)from 2014 to 2020 across China.The multivariate analysis results indicate that fine particulate matter with a diameter of 2.5 μm or less(PM_(2.5))is associated with AMR,accounting for 12%of the variation,followed by residents’income(10.3%)and antibiotic usage density(5.1%).A reduction in PM_(2.5)of 1 μg·m^(-3)is linked to a 0.17%decrease in aggregate antibiotic resistance(p<0.001,R^(2)=0.74).Under different scenarios of China’s PM_(2.5)airquality projections,we further estimated the premature death toll and economic burden derived from PM_(2.5)-related antibiotic resistance in China until 2060.PM_(2.5)-derived AMR is estimated to cause approximately 27000(95%confidence interval(CI):646848830)premature deaths and about 0.51(95%CI;0.12-0.92)million years of life lost annually in China,equivalent to an annual welfare loss of 8.4(95%CI;2.0-15.0)billion USD.Implementing the“Ambitious Pollution 1.5℃ Goals”scenario to reduce PM_(2.5)concentrations could prevent roughly 14000(95%CI;3324-26320)premature deaths—with a potential monetary value of 9.8(95%CI;2.2-17.6)billion USD—from AMR by 2060.These results suggest that reducing air pollution may offer co-benefits in the health and economic sectors by mitigating AMR.展开更多
1.Introduction Adherence to 24-hour movement guidelines—which encompass regular physical activity,adequate sleep,and limited sedentary time1—significantly influences long-term health outcomes during adolescence and ...1.Introduction Adherence to 24-hour movement guidelines—which encompass regular physical activity,adequate sleep,and limited sedentary time1—significantly influences long-term health outcomes during adolescence and contributes holistically to overall health.2 However,its prospective relationship with midlife mortality remains unknown to date.Our aim was to determine the association between adherence to 24-hour movement guidelines during adolescence and premature mortality 26-27 years later.This aim was based on existing evidence3 suggesting a potential link between meeting these guidelines and reduced mortality risk among adults in an 11-year follow-up.展开更多
A survey conducted on the premature bolting of Huarong large leaf mustard from 2018 to 2024 revealed that Huarong large leaf mustard sown in middle August was associated with a higher propensity for premature bolting....A survey conducted on the premature bolting of Huarong large leaf mustard from 2018 to 2024 revealed that Huarong large leaf mustard sown in middle August was associated with a higher propensity for premature bolting. Furthermore, it was observed that the earlier being sown, the greater the rate of premature bolting when being sown prior to middle August. The rate of premature bolting observed in seedlings sown on August 8 was recorded at 35.6%. It was noted that as the age of the seedlings increased, the rate of premature bolting correspondingly increased. There were notable differences in the tolerance of various cultivars to elevated temperatures and prolonged sunlight exposure. For instance, cultivars such as Zhangjie 1 and Sichuan Shaguodi, which exhibit greater heat resistance, did not demonstrate premature bolting when sown in early August. The prolonged exposure to elevated temperatures, drought conditions, and extended periods of sunlight during the seedling stage of Huarong large leaf mustard, coupled with delayed irrigation and transplantation, contributed to the occurrence of premature bolting. The Huarong large leaf mustard, when been sown from late August to early September and transplanted at the appropriate time, exhibited normal growth and development, with no instances of premature bolting observed. It is advisable to select heat-resistant varieties, such as Zhangjie 1, prior to middle August. Huarong large leaf mustard should be sown in early to middle September. Additionally, it is essential to ensure centralized production and timely release of seeds, prompt transplantation and harvesting, and enhance the management of pests and diseases.展开更多
Objective:To study the antioxidant protective effect and mechanism of melatonin on cyclophosphamide-induced premature ovarian failure model mice.Methods:Six-month sexually mature female Kunming mice were taken for one...Objective:To study the antioxidant protective effect and mechanism of melatonin on cyclophosphamide-induced premature ovarian failure model mice.Methods:Six-month sexually mature female Kunming mice were taken for one week of acclimatization and then randomly divided into a normal group,blank control group,drug control group,ovarian premature aging model group,and melatonin intervention low,medium,and high dose group,with 20 mice in each group.We observed the status and body mass of the mice in each group;observed and monitored the estrous cycle by HE staining;measured the diameter and size of the ovaries and weighed the wet weight of the ovaries;observed the morphological changes of the ovaries by HE staining and counted the developing follicles at all levels;detected the levels of serum estradiol(E2),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)by ELISA;measured the levels of serum MDA,SOD,and GSH-PX by antioxidant kit;detected the levels of protein immunoblotting by ELISA;protein immunoblotting(Western blot)to examine the expression of DNA damage-related proteinsγH2AX,p53,and p21 in ovarian tissues.Results:Compared with the control group,mice in the premature ovarian failure model group showed reduced mobility,rough hair,decreased body weight,disorganized estrous cycle,decreased ovarian weight(P<0.05),decreased number of follicles at all levels of development(P<0.05),increased number of atretic follicles(P<0.05),significantly elevated levels of serum FSH and LH,significantly decreased levels of E2(P<0.05),significantly increased levels of serum MDA,significantly lower SOD and GSH-PX levels(P<0.05),and the expression of p53,p21,andγH2AX in ovarian tissues was increased(P<0.05).Compared with the model group of premature ovarian failure,melatonin improved the changes of the above indexes induced by cyclophosphamide-induced premature ovarian failure in mice.Conclusion:Melatonin can improve the changes of motility cycle disorders,abnormal follicular development,and abnormal serum hormone levels induced by cyclophosphamide-induced oxidative stress in mice with premature ovarian failure.At the same time,melatonin can improve the oxidative stress induced by cyclophosphamide and alleviate the role of oxidative stress-induced DNA damage in mouse ovaries by exerting its antioxidant effect.展开更多
Objective:To explore the positive role of developmental care measures in promoting the growth and development of premature infants,and analyze their specific effects on improving the sleep duration and quality of prem...Objective:To explore the positive role of developmental care measures in promoting the growth and development of premature infants,and analyze their specific effects on improving the sleep duration and quality of premature infants.Methods:A total of 80 premature infants who met the inclusion criteria were selected by convenient sampling method and divided into an experimental group and a control group,with 40 cases in each group.The experimental group received comprehensive nursing intervention of developmental care during the nursing process;the control group adopted the traditional conventional nursing model.Results:There was no statistically significant difference in various physical indicators between the two groups of premature infants before nursing intervention.After nursing intervention,the scores of intellectual development level,psychomotor development index and various physical indicators of premature infants in the experimental group were significantly higher than those in the control group,and the difference was statistically significant.Conclusion:The implementation of developmental care for premature infants can significantly improve their various developmental indicators,which can not only effectively promote the rapid development of premature infants’mental and physical growth,but also improve their sleep quality.展开更多
A previous study showed that the length of the foreskin plays a role in the risk of sexually transmitted infections and chronic prostatitis,which can lead to poor quality of sexual life.Here,the association between fo...A previous study showed that the length of the foreskin plays a role in the risk of sexually transmitted infections and chronic prostatitis,which can lead to poor quality of sexual life.Here,the association between foreskin length and sexual dysfunction was evaluated.A total of 5700 participants were recruited from the andrology clinic at The First Affiliated Hospital of University of Science and Technology of China(Hefei,China).Clinical characteristics,including foreskin length,were collected,and sexual function was assessed by the International Index of Erectile Function-5(IIEF-5)and Premature Ejaculation Diagnostic Tool(PEDT)questionnaires.Men with sexual dysfunction were more likely to have redundant foreskin than men without sexual dysfunction.Among the 2721 erectile dysfunction(ED)patients and 1064 premature ejaculation(PE)patients,301(11.1%)ED patients and 135(12.7%)PE patients had redundant foreskin,respectively.Men in the PE group were more likely to have redundant foreskin than men in the non-PE group(P=0.004).Logistic regression analyses revealed that the presence of redundant foreskin was associated with increased odds of moderate/severe ED(adjusted odds ratio[aOR]=1.31,adjusted P=0.04),moderate PE(aOR=1.38,adjusted P=0.02),and probable PE(aOR=1.37,adjusted P=0.03)after adjusting for confounding variables.Our study revealed a positive correlation between the presence of redundant foreskin and the risk of sexual dysfunction,especially in PE patients.Assessment of the length of the foreskin during routine clinical diagnosis may provide information for patients with sexual dysfunction.展开更多
Objective:To explore the correlation between night ECG parameters and sleep quality in elderly patients with atrial premature beat(PAC).Methods:A total of 307 elderly patients with PAC were selected from March 2022 to...Objective:To explore the correlation between night ECG parameters and sleep quality in elderly patients with atrial premature beat(PAC).Methods:A total of 307 elderly patients with PAC were selected from March 2022 to March 2024.The parameters of room morning load and heart rate variability(HRV)at night(22:00-6:00)were collected by 24h holter electrocardiogram,and the sleep quality of PAC patients was evaluated by Pittsburgh Sleep Quality Index(PSQI).Multiple regression analysis was used to explore the correlation between night ECG parameters and sleep quality.Results:The incidence of sleep disorder in 307 elderly PAC patients was 62.54%.Univariate analysis showed that there were no statistically significant differences in gender,BMI and education level(P>0.05),but there were statistically significant differences in age,disease course,underlying diseases,atrial morning load,SDNN,RMSSD,LF,HF,LF/HF and TP(P<0.05).Multivariate Logistic regression analysis showed that atrial morning load,SDNN,RMSSD,LF and LF/HF were independent influencing factors of sleep disorder in elderly PAC patients(P<0.05).Patients with sleep disorders were divided into mild group,moderate group and severe group according to PSQI score,and there were statistically significant differences in the indexes of atrial morning load,SDNN,LF,LF/HF and TP among the three groups(all P<0.05).Spearman correlation analysis showed that room morning load,LF,LF/HF and TP were positively correlated with the degree of sleep disorder,while SDNN parameters were negatively correlated with the degree of sleep disorder(all P<0.05).Conclusion:There is a significant correlation between night ECG parameters and sleep disorders in elderly patients with PAC.It is possible to prevent and treat sleep disorders by monitoring ECG abnormalities and improve the reliability of treatment.展开更多
To review the existing deep learning applications for diagnosing diabetic retinopathy and retinopathy of prematurity diseases,the available public retinal databases for the diseases and apply the International Journal...To review the existing deep learning applications for diagnosing diabetic retinopathy and retinopathy of prematurity diseases,the available public retinal databases for the diseases and apply the International Journal of Medical Informatics(IJMEDI)checklist were assessed the quality of included studies;an in-depth literature search in Scopus,Web of Science,IEEE and ACM databases targeting articles from inception up to 31st January 2023 was done by two independent reviewers.In the review,26 out of 1476 articles with a total of 36 models were included.Data size and model validation were found to be challenges for most studies.Deep learning models are gaining focus in the development of medical diagnosis tools and applications.However,there seems to be a critical issue with most of the studies being published,with some not including information about data sources and data sizes which is important for their performance verification.展开更多
AIM:To compare the changes in preschool refractive status,ocular biological parameters,and higher-order aberrations in children with retinopathy of prematurity(ROP)after retinal laser photocoagulation or anti-vascular...AIM:To compare the changes in preschool refractive status,ocular biological parameters,and higher-order aberrations in children with retinopathy of prematurity(ROP)after retinal laser photocoagulation or anti-vascular endothelial growth factor(anti-VEGF)treatment and explore their underlying factors.METHODS:This observational study involved 118 eyes of 59 children,aged 3 to 6y,with ROP followed up between March 2023 and October 2024.They were divided into the laser,anti-VEGF,and anti-VEGF+laser groups.The laser group received a single session of laser photocoagulation.The anti-VEGF group received a single anti-VEGF treatment.The anti-VEGF+laser group received a single anti-VEGF treatment after birth followed by supplementary laser treatment within 2wk to 6mo.Ocular biological parameters were measured using IOL Master 700 and Pentacam HR.Right-eye higher-order aberrations were measured using the OPD-Scan III.Best-corrected visual acuities(BCVA),refractive statuses,ocular biological parameters,and higherorder aberrations were assessed and compared.Multiple linear regression analysis was conducted to evaluate the relationships among ocular biological parameters,higherorder aberrations,spherical equivalent(SE),and treatment methods.Pearson’s correlation coefficients were used to assess the relationships between the SE and higher-order aberrations.RESULTS:The laser group had a higher incidence of myopia and a lower SE than the anti-VEGF group.The incidence of astigmatism and cylindrical power were significantly lower for the anti-VEGF than for the laser and anti-VEGF+laser groups(P<0.05).The anterior corneal surface astigmatism was higher for the laser and anti-VEGF+laser groups than for the anti-VEGF group.The anterior corneal surface K2 and lens thickness were higher for the laser and anti-VEGF+laser groups than for the anti-VEGF group.The whole-eye higher-order aberration root mean square(RMS)values for the right eye were significantly higher for the laser and anti-VEGF+laser groups than for the anti-VEGF group.The whole-eye trefoil RMS values for the right eye were also significantly higher for the laser and anti-VEGF+laser groups than for the anti-VEGF group(P<0.05).Laser treatment was significantly associated with SE,anterior corneal surface curvature and astigmatism,lens thickness,whole-eye highorder aberrations,and whole-eye trefoil(all P<0.05).CONCLUSION:Children with ROP who received laser treatment have higher myopia and astigmatism than those who received anti-VEGF treatment.Children treated with laser or supplementary laser treatment have higher anterior corneal astigmatism,anterior corneal curvature,thicker lenses,whole-eye higher-order aberrations,and whole-eye trefoil.The cause of myopia in children with ROP after laser treatment is increased anterior corneal surface curvature and lens thickness.展开更多
Age-related osteoporosis poses a significant challenge in musculoskeletal health;a condition characterized by reduced bone density and increased fracture susceptibility in older individuals necessitates a better under...Age-related osteoporosis poses a significant challenge in musculoskeletal health;a condition characterized by reduced bone density and increased fracture susceptibility in older individuals necessitates a better understanding of underlying molecular and cellular mechanisms.Emerging evidence suggests that osteocytes are the pivotal orchestrators of bone remodeling and represent novel therapeutic targets for age-related bone loss.Our study uses the prematurely aged PolgD257A/D257A(PolgA)mouse model to scrutinize age-and sex-related alterations in musculoskeletal health parameters(frailty,grip strength,gait data),bone and particularly the osteocyte lacuno-canalicular network(LCN).Moreover,a new quantitative in silico image analysis pipeline is used to evaluate the alterations in the osteocyte network with aging.Our findings underscore the pronounced degenerative changes in the musculoskeletal health parameters,bone,and osteocyte LCN in PolgA mice as early as 40 weeks,with more prominent alterations evident in aged males.Our findings suggest that the PolgA mouse model serves as a valuable model for studying the cellular mechanisms underlying age-related bone loss,given the comparable aging signs and age-related degeneration of the bone and the osteocyte network observed in naturally aging mice and elderly humans.展开更多
Premature ovarian insufficiency(POI)is characterized by irreversible loss of ovarian function before 40 years of age and affects 3.7%of women worldwide(Golezar et al.,2019;Huang et al.,2021).Raised serum follicle-stim...Premature ovarian insufficiency(POI)is characterized by irreversible loss of ovarian function before 40 years of age and affects 3.7%of women worldwide(Golezar et al.,2019;Huang et al.,2021).Raised serum follicle-stimulating hormone(FSH)levels,menstrual disturbance,and estrogen deficiency are the main symptoms of POl(European Society for Human Reproduction et al.,2016).Besides infertility,patients have an increased risk of long-term complications due to the early deficiency of ovarian steroids,such as osteoporosis,cardiovascular disease,and metabolic disorders(lshizuka,2021).Due to the lack of biomarkers for prediction or early diagnosis,most patients are not diagnosed until the failure stage(serum levels of FSH above 25 IU/L and amenorrhea),missing opportunities for intervention.Therefore,developing sensitive biomarkers for diminished ovarian reserves is paramount for timely treatment to improve reproductive outcomes and prevent long-term complications.展开更多
Objective:To explore the application effect of improved“Bird’s nest”nursing in the transport of premature infants.Methods:Collecting 100 cases of premature infants,randomly divided into experimental group and contr...Objective:To explore the application effect of improved“Bird’s nest”nursing in the transport of premature infants.Methods:Collecting 100 cases of premature infants,randomly divided into experimental group and control group,two groups of premature 50 cases each.The control group received the traditional“Bird’s nest”nursing.Experimental group in modified the“bird’s nest”nursing intervention,compared two groups of premature monitoring vital signs during the transfer process,transport after the body development indicators,the time and cure warmers intervention.Results:Compared with control group,experimental group premature temperature fluctuation is small,apnea and fewer times of low body temperature and oxygen saturation is higher,the contrast results with statistical significance(P<0.05).There was no significant difference in physical development,time to get out of the incubator,and cure rate.Conclusion:Modified the“bird’s nest”care in outside the hospital application,help to improve premature transfer process safety,and security for premature success implementation of transshipment is of great significance.展开更多
Retinopathy of prematurity(ROP)is a vision-threatening disorder that leads to pathological growth of the retinal vasculature due to hypoxia.Here,we investigated the potential effects of alamandine,a novel heptapeptide...Retinopathy of prematurity(ROP)is a vision-threatening disorder that leads to pathological growth of the retinal vasculature due to hypoxia.Here,we investigated the potential effects of alamandine,a novel heptapeptide in the renin-angiotensin system(RAS),on hypoxia-induced retinal neovascularization and its underlying mechanisms.In vivo,the C57BL/6J mice with oxygen-induced retinopathy(OIR)were injected intravitreally with alamandine(1.0µmol/kg per eye).In vitro,human retinal microvascular endothelial cells(HRMECs)were utilized to investigate the effects of alamandine(10µg/mL)on proliferation,apoptosis,migration,and tubular formation under vascular endothelial growth factor(VEGF)stimulation.Single-cell RNA sequencing(scRNA-seq)matrix data from the Gene Expression Omnibus(GEO)database and RAS-related genes from the Molecular Signatures Database(MSigDB)were sourced for subsequent analyses.By integrating scRNA-seq data across multiple species,we identified that RAS-associated endothelial cell populations were highly related to retinal neovascularization.The liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis revealed a significant decrease in alamandine levels in both the serum and retina of OIR mice compared to those in the control group.Next,alamandine ameliorated hypoxia-induced retinal pathological neovascularization and physiologic revascularization in OIR mice.In vitro,alamandine effectively mitigated VEGF-induced proliferation,scratch wound healing,and tube formation of HRMECs primarily by inhibiting the hypoxia-inducible factor-1α(HIF-1α)/VEGF pathway.Further,coincubation with D-Pro7(Mas-related G protein-coupled receptor D(MrgD)antagonist)hindered the beneficial impacts of alamandine on hypoxia-induced pathological angiogenesis both in vivo and in vitro.Our findings suggested that alamandine could mitigate retinal neovascularization by targeting the MrgD-mediated HIF-1α/VEGF pathway,providing a potential therapeutic agent for OIR prevention and treatment.展开更多
文摘Dear Editor,We recently reviewed two important studies that investigate the use of botulinum-A toxin(BoNT-A)injections into the bulbospongiosus muscle as a treatment for lifelong premature ejaculation(PE).While both studies share the goal of evaluating the efficacy and safety of BoNT-A in this context,they reached very different conclusions.The study by Shaher et al.demonstrated significant improvements in ejaculatory latency,indicating that BoNT-A injections may be a helpful treatment for PE.
文摘This study aimed to compare the complications of preterm twins versus singletons and analyze differences across gestational ages.Preterm twins delivered between 2 March 2022 and 6 November 2022 were compared to an age-matched control group of singletons,involving 65 twins and 103 singletons.The most common complication in premature infants was neonatal jaundice(87.72%),followed by patent foramen ovale(79.76%)and neonatal respiratory distress syndrome(NRDS)(57.14%).Twins had significantly higher Apgar scores at 1,5,and 10 minutes compared to singletons.However,twins showed a higher incidence of ventricular septal defect(VSD)(7.69%)than singletons,with a statistically significant difference.In contrast,twins exhibited significantly lower rates of neonatal jaundice(78.46%),electrolyte imbalance(18.4%),and acid-base imbalance(9.23%)compared to singletons.Furthermore,as gestational age increased,the incidence of intrauterine infection,electrolyte and acid-base imbalances,neonatal coagulation disorders,patent ductus arteriosus(PDA),anemia,and NRDS in preterm infants gradually decreased,with all differences reaching statistical significance(P<0.05).These findings highlight the importance of close monitoring and timely management of complications in premature infants to prevent severe outcomes.
基金funded by the Swiss National Science Foundation(SNSF Grant No.320030L_192073 to GM)the Slovenian Research Agency(ARRS Grant No.N5-0152 to TD).
文摘Background: Prematurely-born individuals tend to exhibit higher resting oxidative stress, although evidence suggests they may be more resistant to acute hypoxia-induced redox balance alterations. We aimed to investigate the redox balance changes across a 3-day hypobaric hypoxic exposure at 3375 m in healthy adults born preterm(gestational age ≤ 32 weeks) and their term-born(gestational age ≥ 38 weeks)counterparts.Methods: Resting venous blood was obtained in normoxia(prior to altitude exposure), immediately upon arrival to altitude, and the following 3mornings. Antioxidant(superoxide dismutase(SOD), catalase, glutathione peroxidase(GPx), and ferric reducing antioxidant power(FRAP)),pro-oxidant(xanthine oxidase(XO) and myeloperoxidase(MPO)) enzyme activity, oxidative stress markers(advanced oxidation protein product(AOPP) and malondialdehyde(MDA)), nitric oxide(NO) metabolites(nitrites, nitrates, and total nitrite and nitrate(NOx)), and nitrotyrosine were measured in plasma.Results: SOD increased only in the preterm group(p < 0.05). Catalase increased at arrival in preterm group(p < 0.05). XO activity increased at Day 3 for the preterm group, while it increased acutely(arrival and Day 1) in control group. MPO increased in both groups throughout the3 days(p < 0.05). AOPP only increased at arrival in the preterm(p < 0.05) whereas it decreased at arrival up to Day 3(p < 0.05) for control.MDA decreased in control group from arrival onward. Nitrotyrosine decreased in both groups(p < 0.05). Nitrites increased on Day 3(p < 0.05)in control group and decreased on Day 1(p < 0.05) in preterm group.Conclusion: These data indicate that antioxidant enzymes seem to increase immediately upon hypoxic exposure in preterm adults. Conversely, the blunted pro-oxidant enzyme response to prolonged hypoxia exposure suggests that these enzymes may be less sensitive in preterm individuals.These findings lend further support to the potential hypoxic preconditioning effect of preterm birth.
文摘BACKGROUND Premature infant formula is based on milk and contains energy,vitamins,etc.Breast milk is rich in minerals,such as phosphorus and calcium,and proteins.Both can be used for nutritional support in preterm infants with low-birth-weight.However,their effects on the difference in infant growth rate and postpartum depression are still unclear.AIM To explore the effect of early micro-breastfeeding on the growth rate of preterm infants with low-birth-weight and maternal postpartum depression.METHODS Data of 68 preterm infants with low-birth-weight and their mothers admitted to the Department of Neonatology,Suzhou Ninth Hospital Affiliated to Soochow University,between January 2022 and December 2024,were retrospectively collected.The infants were divided into two groups according to the different enteral feeding methods in the early stage:Control group(n=32 cases,premature infant formula feeding)and observation group(n=36 cases,micro-breastfeeding,i.e.,exclusive breastfeeding,no mixed feeding).The baseline data;feeding status;gastrointestinal adverse reactions such as vomiting,gastric retention,and abdominal distension;growth rate(body weight,body length,and head circumference);and adverse events(infection,cholestasis,hyperbilirubinemia,and necrotizing enterocolitis)were compared between the two groups.Moreover,the maternal postpartum depression status of the two groups based of infants based on the Edinburgh postpartum depression scale(EPDS)was compared.RESULTS No significant difference in baseline data was found between the two groups(P>0.05).The onset time of weight gain(6.49±0.53 days vs 7.09±0.61 days,P<0.001),time for complete meconium excretion(6.28±0.92 days vs 8.31±1.17 days,P<0.001),time for recovery to birth weight(8.81±1.40 days vs 10.95±1.64 days,P<0.001),and time to reach full enteral feeding(12.29±2.08 days vs 15.48±2.27 days,P<0.001)were compared between the observation and control groups.The incidence of vomiting,abdominal distension,and gastric retention was significantly lower in the observation group than in the control group,and the difference was significant(P<0.05).The rates of the weight growth(15.88±1.57 g/day vs 14.84±1.51 g/day,P=0.007),head circumference(0.63±0.08 cm/week vs 0.59±0.05 cm/week,P=0.018),and length(0.80±0.12 cm/week vs 0.73±0.14 cm/week,P=0.029)were compared between the observation and control groups.On hospital admission of preterm infants with low-birthweight,the mothers of the two groups did not show a significant difference in the EPDS scores for postpartum depression(P>0.05).Preterm infants with low-birth-weight were hospitalized for 2 weeks,and the EPDS score for maternal postpartum depression was significantly lower in the observation group than in the control group(8.38±1.47 vs 9.49±2.35,P=0.021).CONCLUSION Compared with preterm infant formula feeding,early micro-breastfeeding can more effectively promote the growth rate of preterm infants with low-birth-weight and reduce the gastrointestinal feeding intolerance and related complications,thereby alleviating mothers’concerns about their children and reducing the risk of postpartum depression.
基金sponsored by the National Natural Science Foundation of China(81673764)National Key R&D Program of China(022YFC3502301).
文摘Objective:To explore the pharmacological effects and molecular mechanisms of a Jiao'ai decoction(JAD)in treating premature ovarian failure.Specifically,we evaluated the receptor for advanced glycation end(RAGE)products pathway,metabolic disorders,and intestinal flora dysbiosis.Methods:Forty female rats with normal estrous cycles were randomly divided into five groups:control,model,estradiol,low-dose JAD,and high-dose JAD,with 8 rats in each.Except for the control group,the rats in other groups were injected intraperitoneally with cisplatin for 8 days(1.5 mg/kg)to establish a premature ovarian failure model.Starting on the fifth day of cisplatin injections,the estradiol,low-dose JAD,and high-dose JAD groups were administered corresponding drugs for 21 days.Sex hormone levels and pathological changes in the ovaries were measured.Key proteins in the RAGE pathway related to apoptosis,aging,and inflammation,were tested using Western blot.A 16S rRNA analysis of feces and non-targeted metabolism in serum was performed to determine the effects of JAD on intestinal flora and metabolism.Results:Body weight,ovarian index,and the number of follicles at all levels increased in the JAD group.Regarding serum hormones,estradiol,anti-mullerian hormone(AMH)and P levels increased,whereas follicle-stimulating hormone(FSH)and luteinizing hormone(LH)levels decreased in the JAD group.The levels of phosphorylated Akt protein(P-Akt),receptor for advanced glycation end products(RAGE),tumor protein p53(P53),C-reactive protein(CRP),apoptosis regulator BAX(BAX)and Caspase3 were downregulated by JAD,whereas B cell leukemia/lymphoma 2(Bcl-2)and Endothelial nitric oxidase synthase(eNOS)were upregulated.JAD was also found to play an important role in the regulation of metabolic disorders and intestinal ecological imbalances by adjusting species composition and diversity.Conclusion:JAD can protect ovaries by exerting anti-inflammatory and anti-apoptotic effects via inhibition of the RAGE pathway.JAD can also regulate metabolic disorders and maintain the dynamic balance of intestinal flora,thereby contributing to the improvement of the ovarian reserve function.
基金supported by National Key Research and Development Program of China(2024YFD2300221)China Agricultural Research System(CARS-15–15)+1 种基金Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2024D03)Dong Hezhong Studio for Popularization of Science and Technology in Salt Tolerant Industrial Crops(202228297).
文摘Premature senescence in Bacillus thuringiensis(Bt)cotton has emerged as a significant challenge to the formation and realization of fiber yield and quality since its commercialization in 1997.Initially,premature senescence was thought to be an inherent trait associated with the Bt gene.However,subsequent research and practice have demonstrated that it is not directly linked to the Bt gene but rather results from a physiological imbalance between the sink and source,as well as between the root and shoot in Bt cotton.This short review provides an overview of the causes,mechanisms,and control measures for premature senescence in Bt cotton.It offers valuable insights for future research and the sustainable application of transgenic crops.
基金Supported by Sainte-Justine University Hospital Research Center excellence grant in 2020-2021.
文摘BACKGROUND Disorders of gut-brain interaction(DGBI)are defined as a variable combination of chronic or recurrent gastrointestinal symptoms.Early-life stressors have been implicated as possible contributing factors.AIM To determine if prematurity and neonatal factors influence the development of DGBI in adults.METHODS A case-control study was carried out at a tertiary referral center from July 2019 to July 2021.Cases(adults born with extremely premature<29 weeks of gestation)were recruited from the Health of Adults Born Preterm Investigation cohort.Control subjects were recruited from the general population.All participants completed the Rome IV diagnostic questionnaire online.Cases completed anxiety and depression questionnaires(Patient-Reported Outcomes Measurement Infor-mation System-29 items,Generalized Anxiety Disorder-7 items,Patient Health Questionnaire-9 items).Neonatal data and sociodemographic status were collected.RESULTS A total of 79 cases and 124 controls were enrolled in the study.The group of adults born preterm exhibited a significantly higher prevalence of functional bowel disorders(P=0.01)and a trend suggesting a higher prevalence of func-tional gastroduodenal disorders(P=0.06).Among women born prematurely,the prevalence of functional gastroduodenal disorders,functional bowel disorders,and functional constipation was significantly higher compared to the female control group(P=0.02 for all).The identified risk factors are categorized as directly linked to prematurity(e.g.,chorioamnionitis),indirectly related to prematurity(e.g.,anxiety,depression,and social skills as consequences of prematurity),or independent of prematurity(e.g.,female sex).CONCLUSION This is the first case-control study reporting the prevalence of DGBI in a cohort of well-characterized adults born prematurely.We confirm that prematurity is a risk factor for developing a DGBI.
基金funded by the National Natural Science Foun-dation of China(22406168,W2411031,and 52270201)the China Postdoctoral Science Foundation(2023M733061)the Zhejiang University Global Partnership Fund(100000-11320/198).
文摘The One Health concept acknowledges the importance of multiple dimensions in controlling antimicrobial resistance(AMR).However,our understanding of how anthropological,socioeconomic,and environmental factors drive AMR at a national level remains limited.To explore associations between potential contributing factors and AMR,this study analyzed an extensive database comprising 13 major antibioticresistant bacteria and over 30 predictors(e.g.,air pollution,antibiotic usage,economy,husbandry,public services,health services,education,diet,climate,and population)from 2014 to 2020 across China.The multivariate analysis results indicate that fine particulate matter with a diameter of 2.5 μm or less(PM_(2.5))is associated with AMR,accounting for 12%of the variation,followed by residents’income(10.3%)and antibiotic usage density(5.1%).A reduction in PM_(2.5)of 1 μg·m^(-3)is linked to a 0.17%decrease in aggregate antibiotic resistance(p<0.001,R^(2)=0.74).Under different scenarios of China’s PM_(2.5)airquality projections,we further estimated the premature death toll and economic burden derived from PM_(2.5)-related antibiotic resistance in China until 2060.PM_(2.5)-derived AMR is estimated to cause approximately 27000(95%confidence interval(CI):646848830)premature deaths and about 0.51(95%CI;0.12-0.92)million years of life lost annually in China,equivalent to an annual welfare loss of 8.4(95%CI;2.0-15.0)billion USD.Implementing the“Ambitious Pollution 1.5℃ Goals”scenario to reduce PM_(2.5)concentrations could prevent roughly 14000(95%CI;3324-26320)premature deaths—with a potential monetary value of 9.8(95%CI;2.2-17.6)billion USD—from AMR by 2060.These results suggest that reducing air pollution may offer co-benefits in the health and economic sectors by mitigating AMR.
文摘1.Introduction Adherence to 24-hour movement guidelines—which encompass regular physical activity,adequate sleep,and limited sedentary time1—significantly influences long-term health outcomes during adolescence and contributes holistically to overall health.2 However,its prospective relationship with midlife mortality remains unknown to date.Our aim was to determine the association between adherence to 24-hour movement guidelines during adolescence and premature mortality 26-27 years later.This aim was based on existing evidence3 suggesting a potential link between meeting these guidelines and reduced mortality risk among adults in an 11-year follow-up.
基金Supported by Key R&D Projects of Hunan Provincial Department of Science and Technology"Study on Key Modern Processing Techniques and Product Development of Huarong Mustard"(2023NK2039).
文摘A survey conducted on the premature bolting of Huarong large leaf mustard from 2018 to 2024 revealed that Huarong large leaf mustard sown in middle August was associated with a higher propensity for premature bolting. Furthermore, it was observed that the earlier being sown, the greater the rate of premature bolting when being sown prior to middle August. The rate of premature bolting observed in seedlings sown on August 8 was recorded at 35.6%. It was noted that as the age of the seedlings increased, the rate of premature bolting correspondingly increased. There were notable differences in the tolerance of various cultivars to elevated temperatures and prolonged sunlight exposure. For instance, cultivars such as Zhangjie 1 and Sichuan Shaguodi, which exhibit greater heat resistance, did not demonstrate premature bolting when sown in early August. The prolonged exposure to elevated temperatures, drought conditions, and extended periods of sunlight during the seedling stage of Huarong large leaf mustard, coupled with delayed irrigation and transplantation, contributed to the occurrence of premature bolting. The Huarong large leaf mustard, when been sown from late August to early September and transplanted at the appropriate time, exhibited normal growth and development, with no instances of premature bolting observed. It is advisable to select heat-resistant varieties, such as Zhangjie 1, prior to middle August. Huarong large leaf mustard should be sown in early to middle September. Additionally, it is essential to ensure centralized production and timely release of seeds, prompt transplantation and harvesting, and enhance the management of pests and diseases.
基金2023 Special Project for Serving the National Development Strategy with Basic Scientific Research Fees from Central Universities(No.31920230188)2023 Northwestern Minzu University College-Level Innovation and Entrepreneurship Training Program(No.X202310742289)2024 National College Students’Innovation and Entrepreneurship Training Program(No.202410742005)。
文摘Objective:To study the antioxidant protective effect and mechanism of melatonin on cyclophosphamide-induced premature ovarian failure model mice.Methods:Six-month sexually mature female Kunming mice were taken for one week of acclimatization and then randomly divided into a normal group,blank control group,drug control group,ovarian premature aging model group,and melatonin intervention low,medium,and high dose group,with 20 mice in each group.We observed the status and body mass of the mice in each group;observed and monitored the estrous cycle by HE staining;measured the diameter and size of the ovaries and weighed the wet weight of the ovaries;observed the morphological changes of the ovaries by HE staining and counted the developing follicles at all levels;detected the levels of serum estradiol(E2),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)by ELISA;measured the levels of serum MDA,SOD,and GSH-PX by antioxidant kit;detected the levels of protein immunoblotting by ELISA;protein immunoblotting(Western blot)to examine the expression of DNA damage-related proteinsγH2AX,p53,and p21 in ovarian tissues.Results:Compared with the control group,mice in the premature ovarian failure model group showed reduced mobility,rough hair,decreased body weight,disorganized estrous cycle,decreased ovarian weight(P<0.05),decreased number of follicles at all levels of development(P<0.05),increased number of atretic follicles(P<0.05),significantly elevated levels of serum FSH and LH,significantly decreased levels of E2(P<0.05),significantly increased levels of serum MDA,significantly lower SOD and GSH-PX levels(P<0.05),and the expression of p53,p21,andγH2AX in ovarian tissues was increased(P<0.05).Compared with the model group of premature ovarian failure,melatonin improved the changes of the above indexes induced by cyclophosphamide-induced premature ovarian failure in mice.Conclusion:Melatonin can improve the changes of motility cycle disorders,abnormal follicular development,and abnormal serum hormone levels induced by cyclophosphamide-induced oxidative stress in mice with premature ovarian failure.At the same time,melatonin can improve the oxidative stress induced by cyclophosphamide and alleviate the role of oxidative stress-induced DNA damage in mouse ovaries by exerting its antioxidant effect.
文摘Objective:To explore the positive role of developmental care measures in promoting the growth and development of premature infants,and analyze their specific effects on improving the sleep duration and quality of premature infants.Methods:A total of 80 premature infants who met the inclusion criteria were selected by convenient sampling method and divided into an experimental group and a control group,with 40 cases in each group.The experimental group received comprehensive nursing intervention of developmental care during the nursing process;the control group adopted the traditional conventional nursing model.Results:There was no statistically significant difference in various physical indicators between the two groups of premature infants before nursing intervention.After nursing intervention,the scores of intellectual development level,psychomotor development index and various physical indicators of premature infants in the experimental group were significantly higher than those in the control group,and the difference was statistically significant.Conclusion:The implementation of developmental care for premature infants can significantly improve their various developmental indicators,which can not only effectively promote the rapid development of premature infants’mental and physical growth,but also improve their sleep quality.
基金supported by the National Natural Science Foundation of China(No.81901543 and No.81971333)the Key Research and Development Project of Anhui Province(2022e07020014)the Joint Fund for Medical Artificial Intelligence(MAI2022Q010).
文摘A previous study showed that the length of the foreskin plays a role in the risk of sexually transmitted infections and chronic prostatitis,which can lead to poor quality of sexual life.Here,the association between foreskin length and sexual dysfunction was evaluated.A total of 5700 participants were recruited from the andrology clinic at The First Affiliated Hospital of University of Science and Technology of China(Hefei,China).Clinical characteristics,including foreskin length,were collected,and sexual function was assessed by the International Index of Erectile Function-5(IIEF-5)and Premature Ejaculation Diagnostic Tool(PEDT)questionnaires.Men with sexual dysfunction were more likely to have redundant foreskin than men without sexual dysfunction.Among the 2721 erectile dysfunction(ED)patients and 1064 premature ejaculation(PE)patients,301(11.1%)ED patients and 135(12.7%)PE patients had redundant foreskin,respectively.Men in the PE group were more likely to have redundant foreskin than men in the non-PE group(P=0.004).Logistic regression analyses revealed that the presence of redundant foreskin was associated with increased odds of moderate/severe ED(adjusted odds ratio[aOR]=1.31,adjusted P=0.04),moderate PE(aOR=1.38,adjusted P=0.02),and probable PE(aOR=1.37,adjusted P=0.03)after adjusting for confounding variables.Our study revealed a positive correlation between the presence of redundant foreskin and the risk of sexual dysfunction,especially in PE patients.Assessment of the length of the foreskin during routine clinical diagnosis may provide information for patients with sexual dysfunction.
文摘Objective:To explore the correlation between night ECG parameters and sleep quality in elderly patients with atrial premature beat(PAC).Methods:A total of 307 elderly patients with PAC were selected from March 2022 to March 2024.The parameters of room morning load and heart rate variability(HRV)at night(22:00-6:00)were collected by 24h holter electrocardiogram,and the sleep quality of PAC patients was evaluated by Pittsburgh Sleep Quality Index(PSQI).Multiple regression analysis was used to explore the correlation between night ECG parameters and sleep quality.Results:The incidence of sleep disorder in 307 elderly PAC patients was 62.54%.Univariate analysis showed that there were no statistically significant differences in gender,BMI and education level(P>0.05),but there were statistically significant differences in age,disease course,underlying diseases,atrial morning load,SDNN,RMSSD,LF,HF,LF/HF and TP(P<0.05).Multivariate Logistic regression analysis showed that atrial morning load,SDNN,RMSSD,LF and LF/HF were independent influencing factors of sleep disorder in elderly PAC patients(P<0.05).Patients with sleep disorders were divided into mild group,moderate group and severe group according to PSQI score,and there were statistically significant differences in the indexes of atrial morning load,SDNN,LF,LF/HF and TP among the three groups(all P<0.05).Spearman correlation analysis showed that room morning load,LF,LF/HF and TP were positively correlated with the degree of sleep disorder,while SDNN parameters were negatively correlated with the degree of sleep disorder(all P<0.05).Conclusion:There is a significant correlation between night ECG parameters and sleep disorders in elderly patients with PAC.It is possible to prevent and treat sleep disorders by monitoring ECG abnormalities and improve the reliability of treatment.
基金Supported by DAAD,Google Research,and the Organization for Women in Science for the Developing World(OWSD).
文摘To review the existing deep learning applications for diagnosing diabetic retinopathy and retinopathy of prematurity diseases,the available public retinal databases for the diseases and apply the International Journal of Medical Informatics(IJMEDI)checklist were assessed the quality of included studies;an in-depth literature search in Scopus,Web of Science,IEEE and ACM databases targeting articles from inception up to 31st January 2023 was done by two independent reviewers.In the review,26 out of 1476 articles with a total of 36 models were included.Data size and model validation were found to be challenges for most studies.Deep learning models are gaining focus in the development of medical diagnosis tools and applications.However,there seems to be a critical issue with most of the studies being published,with some not including information about data sources and data sizes which is important for their performance verification.
基金Supported by Key Research and Development Program of Shaanxi Province(No.2024SF-YBXM-320)the Clinical Research Project of the Fourth Military Medical University(No.2022LC2247)Medical Staff Training Boosting Project of Xijing Hospital,Air Force Medical University(No.XJZT24CY52).
文摘AIM:To compare the changes in preschool refractive status,ocular biological parameters,and higher-order aberrations in children with retinopathy of prematurity(ROP)after retinal laser photocoagulation or anti-vascular endothelial growth factor(anti-VEGF)treatment and explore their underlying factors.METHODS:This observational study involved 118 eyes of 59 children,aged 3 to 6y,with ROP followed up between March 2023 and October 2024.They were divided into the laser,anti-VEGF,and anti-VEGF+laser groups.The laser group received a single session of laser photocoagulation.The anti-VEGF group received a single anti-VEGF treatment.The anti-VEGF+laser group received a single anti-VEGF treatment after birth followed by supplementary laser treatment within 2wk to 6mo.Ocular biological parameters were measured using IOL Master 700 and Pentacam HR.Right-eye higher-order aberrations were measured using the OPD-Scan III.Best-corrected visual acuities(BCVA),refractive statuses,ocular biological parameters,and higherorder aberrations were assessed and compared.Multiple linear regression analysis was conducted to evaluate the relationships among ocular biological parameters,higherorder aberrations,spherical equivalent(SE),and treatment methods.Pearson’s correlation coefficients were used to assess the relationships between the SE and higher-order aberrations.RESULTS:The laser group had a higher incidence of myopia and a lower SE than the anti-VEGF group.The incidence of astigmatism and cylindrical power were significantly lower for the anti-VEGF than for the laser and anti-VEGF+laser groups(P<0.05).The anterior corneal surface astigmatism was higher for the laser and anti-VEGF+laser groups than for the anti-VEGF group.The anterior corneal surface K2 and lens thickness were higher for the laser and anti-VEGF+laser groups than for the anti-VEGF group.The whole-eye higher-order aberration root mean square(RMS)values for the right eye were significantly higher for the laser and anti-VEGF+laser groups than for the anti-VEGF group.The whole-eye trefoil RMS values for the right eye were also significantly higher for the laser and anti-VEGF+laser groups than for the anti-VEGF group(P<0.05).Laser treatment was significantly associated with SE,anterior corneal surface curvature and astigmatism,lens thickness,whole-eye highorder aberrations,and whole-eye trefoil(all P<0.05).CONCLUSION:Children with ROP who received laser treatment have higher myopia and astigmatism than those who received anti-VEGF treatment.Children treated with laser or supplementary laser treatment have higher anterior corneal astigmatism,anterior corneal curvature,thicker lenses,whole-eye higher-order aberrations,and whole-eye trefoil.The cause of myopia in children with ROP after laser treatment is increased anterior corneal surface curvature and lens thickness.
基金the European Research Council(ERC Advanced MechAGE-ERC-2016-ADG-741883)the Swiss National Science Foundation(no.188522).
文摘Age-related osteoporosis poses a significant challenge in musculoskeletal health;a condition characterized by reduced bone density and increased fracture susceptibility in older individuals necessitates a better understanding of underlying molecular and cellular mechanisms.Emerging evidence suggests that osteocytes are the pivotal orchestrators of bone remodeling and represent novel therapeutic targets for age-related bone loss.Our study uses the prematurely aged PolgD257A/D257A(PolgA)mouse model to scrutinize age-and sex-related alterations in musculoskeletal health parameters(frailty,grip strength,gait data),bone and particularly the osteocyte lacuno-canalicular network(LCN).Moreover,a new quantitative in silico image analysis pipeline is used to evaluate the alterations in the osteocyte network with aging.Our findings underscore the pronounced degenerative changes in the musculoskeletal health parameters,bone,and osteocyte LCN in PolgA mice as early as 40 weeks,with more prominent alterations evident in aged males.Our findings suggest that the PolgA mouse model serves as a valuable model for studying the cellular mechanisms underlying age-related bone loss,given the comparable aging signs and age-related degeneration of the bone and the osteocyte network observed in naturally aging mice and elderly humans.
基金supported by the National Key Research&Developmental Program of China(2022YFC2703800,2022YFC2703000)the National Natural Science Foundation of China(82125014,82371646,32070847)+1 种基金Basic Science Center Program of NSFC(31988101)the Natural Science Foundation of Shandong Province(ZR202105250005,ZR2022YQ69,tsqn202211371).
文摘Premature ovarian insufficiency(POI)is characterized by irreversible loss of ovarian function before 40 years of age and affects 3.7%of women worldwide(Golezar et al.,2019;Huang et al.,2021).Raised serum follicle-stimulating hormone(FSH)levels,menstrual disturbance,and estrogen deficiency are the main symptoms of POl(European Society for Human Reproduction et al.,2016).Besides infertility,patients have an increased risk of long-term complications due to the early deficiency of ovarian steroids,such as osteoporosis,cardiovascular disease,and metabolic disorders(lshizuka,2021).Due to the lack of biomarkers for prediction or early diagnosis,most patients are not diagnosed until the failure stage(serum levels of FSH above 25 IU/L and amenorrhea),missing opportunities for intervention.Therefore,developing sensitive biomarkers for diminished ovarian reserves is paramount for timely treatment to improve reproductive outcomes and prevent long-term complications.
基金Health Commission of Guangxi Zhuang Autonomous Region,Self-Financing Scientific Research Subject(Project No.:Z20210327)。
文摘Objective:To explore the application effect of improved“Bird’s nest”nursing in the transport of premature infants.Methods:Collecting 100 cases of premature infants,randomly divided into experimental group and control group,two groups of premature 50 cases each.The control group received the traditional“Bird’s nest”nursing.Experimental group in modified the“bird’s nest”nursing intervention,compared two groups of premature monitoring vital signs during the transfer process,transport after the body development indicators,the time and cure warmers intervention.Results:Compared with control group,experimental group premature temperature fluctuation is small,apnea and fewer times of low body temperature and oxygen saturation is higher,the contrast results with statistical significance(P<0.05).There was no significant difference in physical development,time to get out of the incubator,and cure rate.Conclusion:Modified the“bird’s nest”care in outside the hospital application,help to improve premature transfer process safety,and security for premature success implementation of transshipment is of great significance.
基金supported by the National Natural Science Foundation of China(Nos.82200379 and 82300309)the Shanghai Sailing Program(No.22YF1443100)+1 种基金the Academy Talent Special Fund of The First Affiliated Hospital of Nanjing Medical University(Nos.YNRCQN0312 and MXJL202208)the Jiangsu Funding Program for Excellent Postdoctoral Talent(No.2023ZB592),China.
文摘Retinopathy of prematurity(ROP)is a vision-threatening disorder that leads to pathological growth of the retinal vasculature due to hypoxia.Here,we investigated the potential effects of alamandine,a novel heptapeptide in the renin-angiotensin system(RAS),on hypoxia-induced retinal neovascularization and its underlying mechanisms.In vivo,the C57BL/6J mice with oxygen-induced retinopathy(OIR)were injected intravitreally with alamandine(1.0µmol/kg per eye).In vitro,human retinal microvascular endothelial cells(HRMECs)were utilized to investigate the effects of alamandine(10µg/mL)on proliferation,apoptosis,migration,and tubular formation under vascular endothelial growth factor(VEGF)stimulation.Single-cell RNA sequencing(scRNA-seq)matrix data from the Gene Expression Omnibus(GEO)database and RAS-related genes from the Molecular Signatures Database(MSigDB)were sourced for subsequent analyses.By integrating scRNA-seq data across multiple species,we identified that RAS-associated endothelial cell populations were highly related to retinal neovascularization.The liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis revealed a significant decrease in alamandine levels in both the serum and retina of OIR mice compared to those in the control group.Next,alamandine ameliorated hypoxia-induced retinal pathological neovascularization and physiologic revascularization in OIR mice.In vitro,alamandine effectively mitigated VEGF-induced proliferation,scratch wound healing,and tube formation of HRMECs primarily by inhibiting the hypoxia-inducible factor-1α(HIF-1α)/VEGF pathway.Further,coincubation with D-Pro7(Mas-related G protein-coupled receptor D(MrgD)antagonist)hindered the beneficial impacts of alamandine on hypoxia-induced pathological angiogenesis both in vivo and in vitro.Our findings suggested that alamandine could mitigate retinal neovascularization by targeting the MrgD-mediated HIF-1α/VEGF pathway,providing a potential therapeutic agent for OIR prevention and treatment.
