Understanding the cellular origins and early evolutionary dynamics that drive the initiation of carcinogenesis is critical to advancing early detection and prevention strategies.By characterizing key molecular,cellula...Understanding the cellular origins and early evolutionary dynamics that drive the initiation of carcinogenesis is critical to advancing early detection and prevention strategies.By characterizing key molecular,cellular and niche events at the precancerous tipping point of early gastric cancer(EGC),we aimed to develop more precise screening tools and design targeted interventions to prevent malignant transformation at this stage.We utilized our AI models to integrate spatial multimodal data from nine EGC endoscopic submucosal dissection(ESD)samples(covering sequential stages from normal to cancer),construct a spatial-temporal profile of disease progression,and identify a critical tipping point(PMC_P)characterized by an immune-suppressive microenvironment during early cancer development.At this stage,inflammatory pit mucous cells with stemness(PMC_2)interact with fibroblasts via NAMPT→ITGA5/ITGB1 and with macrophages via AREG→EGFR/ERBB2 signaling,fostering cancer initiation.We established gastric precancerous cell lines and organoids to demonstrate that NAMPT and AREG promote cellular proliferation in vitro.Furthermore,in the transgenic CEA-SV40 mouse model,targeting AREG and/or NAMPT disrupted key cell interactions,inhibited the JAK-STAT,MAPK,and NFκB pathways,and reduced PD-L1 expression,which was also confirmed by western blot in vitro.These interventions delayed disease progression,reversed the immunosuppressive microenvironment,and prevented malignant transformation.Clinical validation was conducted using endoscopically resected EGC specimens.Our study provides a precise spatiotemporal depiction of EGC development and identifies novel diagnostic markers and therapeutic targets for early intervention.展开更多
基金supported by Shanghai Oriental Talent Youth Program(QNKJ2024006)National Natural Science Foundation of China(82170555,32300523,32570769,and 62132015)+1 种基金Shanghai Academic/Technology Research Leader(22XD1422400)Shuguang Program of Shanghai Education Development Foundation and Shanghai Municipal Education Commission(22SG06).
文摘Understanding the cellular origins and early evolutionary dynamics that drive the initiation of carcinogenesis is critical to advancing early detection and prevention strategies.By characterizing key molecular,cellular and niche events at the precancerous tipping point of early gastric cancer(EGC),we aimed to develop more precise screening tools and design targeted interventions to prevent malignant transformation at this stage.We utilized our AI models to integrate spatial multimodal data from nine EGC endoscopic submucosal dissection(ESD)samples(covering sequential stages from normal to cancer),construct a spatial-temporal profile of disease progression,and identify a critical tipping point(PMC_P)characterized by an immune-suppressive microenvironment during early cancer development.At this stage,inflammatory pit mucous cells with stemness(PMC_2)interact with fibroblasts via NAMPT→ITGA5/ITGB1 and with macrophages via AREG→EGFR/ERBB2 signaling,fostering cancer initiation.We established gastric precancerous cell lines and organoids to demonstrate that NAMPT and AREG promote cellular proliferation in vitro.Furthermore,in the transgenic CEA-SV40 mouse model,targeting AREG and/or NAMPT disrupted key cell interactions,inhibited the JAK-STAT,MAPK,and NFκB pathways,and reduced PD-L1 expression,which was also confirmed by western blot in vitro.These interventions delayed disease progression,reversed the immunosuppressive microenvironment,and prevented malignant transformation.Clinical validation was conducted using endoscopically resected EGC specimens.Our study provides a precise spatiotemporal depiction of EGC development and identifies novel diagnostic markers and therapeutic targets for early intervention.
