Objective: To study the relationship of the mutation of HBV preS/S gene in HBsAg carrying pregnant women and intrauterine transmission. Methods: Polymerase chain reaction (PCR) was used to amplify HBV preS/S gene from...Objective: To study the relationship of the mutation of HBV preS/S gene in HBsAg carrying pregnant women and intrauterine transmission. Methods: Polymerase chain reaction (PCR) was used to amplify HBV preS/S gene from sera of 8 HBsAg carrying pregnant women, 4 women's neonates infected with HBV, and the other's neonates non-infected with. The PCR products were cloned and 5 clones were chosen from every woman for DNA sequencing. Results: Heterogeneity of HBV preS/S gene in HBsAg carrying pregnant women having intrauterine transmission was much higher than that from having not intrauterine transmission, and the divergence rate of nucleotide sequences also higher strikingly. Conclusion: High heterogeneity of HBV preS/S gene of HBsAg positive pregnant women may be relative to high rate of intrauterine transmis-sion展开更多
Objective:To investigate the relationship between variation of HBV preS genes and clinical consequencesof HBV infection. Methods: We selected 3 groups (3 in each) of HBV infected individuals including those of acutein...Objective:To investigate the relationship between variation of HBV preS genes and clinical consequencesof HBV infection. Methods: We selected 3 groups (3 in each) of HBV infected individuals including those of acuteinfection with complete recovery, chronic HBV carriers and chronic severe hepatitis B. The preS genes were amplified from serum samples with half nested PCR. The PCR products were cloned into M13 vectors and 10 cloneswere randomly selected for each individual and sequenced with standard methods. A total of 90 clones were sequenced and analyzed by DNA homological comparison. Results: The characteristics of the preS genes were significantly different in HBV-infected individuals with different clinical consequences.①As for acute hepatitis B, thepreS function domains were stable and no changes in epitopes for T and B cells were found. ②For chronic HBVcarriers, the function domains within preS proteins showed potentially changes with epitope drifts of T and B cellsbecause of the high variation of HBV preS genes. ③For chronic severe hepatitis B, there were special changes inthe function domains of B cells. The abnormally high immune response would cause severe liver injury in these individuals. Conclusion: This study provides experimental evidence for researches about the possible association ofvariation of preS1/S2 functional regions with clinical consequences.展开更多
The tumorigenesis of hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC) has been widely studied. HBV envelope proteins are important for the structure and life cycle of HBV, and these proteins are useful ...The tumorigenesis of hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC) has been widely studied. HBV envelope proteins are important for the structure and life cycle of HBV, and these proteins are useful for judging the natural disease course and guiding treatment. Truncated and mutated pre S/S are produced by integrated viral sequences that are defective for replication. The pre S/S mutants are considered "precursor lesions" of HCC. Different pre S/S mutants induce various mechanisms of tumorigenesis, such as transactivation of transcription factors and an immune inflammatory response, thereby contributing to HCC. The pre S2 mutants and type Ⅱ "Ground Glass" hepatocytes represent novel biomarkers of HBVassociated HCC. The pre S mutants may induce the unfolded protein response and endoplasmic reticulum stress-dependent and stress-independent pathways. Treatments to inhibit hepatitis B surface antigen(HBs Ag) and damage secondary to HBs Ag or the pre S/S mutants include antivirals and antioxidants, such as silymarin, resveratrol, and glycyrrhizin acid. Methods for the prevention and treatment of HCC should be comprehensive.展开更多
基金Supported by the National Natural Science Foundation of China (No. 39970652)
文摘Objective: To study the relationship of the mutation of HBV preS/S gene in HBsAg carrying pregnant women and intrauterine transmission. Methods: Polymerase chain reaction (PCR) was used to amplify HBV preS/S gene from sera of 8 HBsAg carrying pregnant women, 4 women's neonates infected with HBV, and the other's neonates non-infected with. The PCR products were cloned and 5 clones were chosen from every woman for DNA sequencing. Results: Heterogeneity of HBV preS/S gene in HBsAg carrying pregnant women having intrauterine transmission was much higher than that from having not intrauterine transmission, and the divergence rate of nucleotide sequences also higher strikingly. Conclusion: High heterogeneity of HBV preS/S gene of HBsAg positive pregnant women may be relative to high rate of intrauterine transmis-sion
文摘Objective:To investigate the relationship between variation of HBV preS genes and clinical consequencesof HBV infection. Methods: We selected 3 groups (3 in each) of HBV infected individuals including those of acuteinfection with complete recovery, chronic HBV carriers and chronic severe hepatitis B. The preS genes were amplified from serum samples with half nested PCR. The PCR products were cloned into M13 vectors and 10 cloneswere randomly selected for each individual and sequenced with standard methods. A total of 90 clones were sequenced and analyzed by DNA homological comparison. Results: The characteristics of the preS genes were significantly different in HBV-infected individuals with different clinical consequences.①As for acute hepatitis B, thepreS function domains were stable and no changes in epitopes for T and B cells were found. ②For chronic HBVcarriers, the function domains within preS proteins showed potentially changes with epitope drifts of T and B cellsbecause of the high variation of HBV preS genes. ③For chronic severe hepatitis B, there were special changes inthe function domains of B cells. The abnormally high immune response would cause severe liver injury in these individuals. Conclusion: This study provides experimental evidence for researches about the possible association ofvariation of preS1/S2 functional regions with clinical consequences.
基金Supported by Science and Technology Planning Project of Guangdong ProvinceChinaNo.2014A020212073
文摘The tumorigenesis of hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC) has been widely studied. HBV envelope proteins are important for the structure and life cycle of HBV, and these proteins are useful for judging the natural disease course and guiding treatment. Truncated and mutated pre S/S are produced by integrated viral sequences that are defective for replication. The pre S/S mutants are considered "precursor lesions" of HCC. Different pre S/S mutants induce various mechanisms of tumorigenesis, such as transactivation of transcription factors and an immune inflammatory response, thereby contributing to HCC. The pre S2 mutants and type Ⅱ "Ground Glass" hepatocytes represent novel biomarkers of HBVassociated HCC. The pre S mutants may induce the unfolded protein response and endoplasmic reticulum stress-dependent and stress-independent pathways. Treatments to inhibit hepatitis B surface antigen(HBs Ag) and damage secondary to HBs Ag or the pre S/S mutants include antivirals and antioxidants, such as silymarin, resveratrol, and glycyrrhizin acid. Methods for the prevention and treatment of HCC should be comprehensive.
