Due to its simplicity, high efficiency, and chemo-selectivity, bioorthogonal chemistry has shown a great application potential in pre-targeting.Currently, four bioorthogonal pairs as targeting tools, including (strept...Due to its simplicity, high efficiency, and chemo-selectivity, bioorthogonal chemistry has shown a great application potential in pre-targeting.Currently, four bioorthogonal pairs as targeting tools, including (strept)avidin/biotin, antibody/antigen, oligonucleotide hybridization and IEDDA tools, have been developed and applied in targeted delivery.Nevertheless, all of these tools still suffer from some limitations, such as difficult modification, biochemical fragility and larger molecular weight for biological association tools, as well as chemical instability for IEDDA tools.Synthetic host-vip pairs with relatively small molecular sizes not only possess strong chemical stability, but also have the features of fast conjugation rate, tunable binding affinity , easy modification, and high chemo-selectivity.Consequently, they can be used as a novel non-covalent bioorthogonal tool for pre-targeting.In order to further promote the development of host-vip pairs as novel bioorthogonal tools for pre-targeted delivery, we firstly calculate their conversion rate to make researcher aware of their unique advantages;next, we summarize the recent research progress in this area.The future perspectives and limitations of these unique tools will be discussed.This review will provide a systemic overview of the development of synthetic host-vip pairs as novel bioorthogonal tools for pre-targeting, and may serve as a “go for” resort for researchers who are interested in searching for new synthetic tools to improve pre-targeting.展开更多
With their high drug-loading capacity and enhanced permeability and retention(EPR)effects,nanoparticles possess significant potential for the diagnosis and treatment of tumors.However,unlike active targeting,the compl...With their high drug-loading capacity and enhanced permeability and retention(EPR)effects,nanoparticles possess significant potential for the diagnosis and treatment of tumors.However,unlike active targeting,the complex tumor microenvironment influences the passive accumulation of nanoparticles in tumor areas.Hence,it is necessary to actively control the behavior of nanoparticles when they enter the tumor microenvironment.By utilizing biocompatible and efficient click reactions,the aggregation of nanoparticles at the tumor site can be controlled,thereby enhancing nanoparticle accumulation at the target location with improved imaging signals and enhanced tumor-inhibitory effects.Herein,we introduce and classify in situ nanoparticle aggregation for biomedical imaging and therapeutic applications induced by four types of common click reactions:coppercatalyzed azide–alkyne cycloaddition(CuAAC),strain-promoted azide–alkyne cycloaddition(SPAAC),click condensation between 2-cyanobenzothiazole(CBT)and cysteine(Cys),and inverse electron-demand Diels–Alder(iEDDA).Furthermore,we summarize the main strategies of these click reaction-based nanoparticle aggregation approaches.Finally,we discuss the advantages and disadvantages of click reaction-triggered aggregation and analyze future trends.展开更多
基金supported by the Science and Technology Program of Guangzhou(No.202103000089)the National Natural Science Foundation of China(Nos.22271323 and 22071275)+2 种基金the Innovation Team Project of Universities in Guangdong Province(No.2020KCXTD009)the Scientific and Technological Innovation Leading Talent Project of Zhongshan City(No.LJ2021009)the Key Projects of Social Welfare and Basic Research of Zhongshan City(No.2021B2012).
文摘Due to its simplicity, high efficiency, and chemo-selectivity, bioorthogonal chemistry has shown a great application potential in pre-targeting.Currently, four bioorthogonal pairs as targeting tools, including (strept)avidin/biotin, antibody/antigen, oligonucleotide hybridization and IEDDA tools, have been developed and applied in targeted delivery.Nevertheless, all of these tools still suffer from some limitations, such as difficult modification, biochemical fragility and larger molecular weight for biological association tools, as well as chemical instability for IEDDA tools.Synthetic host-vip pairs with relatively small molecular sizes not only possess strong chemical stability, but also have the features of fast conjugation rate, tunable binding affinity , easy modification, and high chemo-selectivity.Consequently, they can be used as a novel non-covalent bioorthogonal tool for pre-targeting.In order to further promote the development of host-vip pairs as novel bioorthogonal tools for pre-targeted delivery, we firstly calculate their conversion rate to make researcher aware of their unique advantages;next, we summarize the recent research progress in this area.The future perspectives and limitations of these unique tools will be discussed.This review will provide a systemic overview of the development of synthetic host-vip pairs as novel bioorthogonal tools for pre-targeting, and may serve as a “go for” resort for researchers who are interested in searching for new synthetic tools to improve pre-targeting.
基金supported by the National Key Research and Development Program of China(Grant 2023YFF0724100)National Natural Science Foundation of China(Grants 22074016 and 22234002)+1 种基金Natural Science Foundation of Jiangsu Province(Grant BK20232007)the Full-time Talents Program of Hebei Province(Grant 2023HBQZYCXY027).
文摘With their high drug-loading capacity and enhanced permeability and retention(EPR)effects,nanoparticles possess significant potential for the diagnosis and treatment of tumors.However,unlike active targeting,the complex tumor microenvironment influences the passive accumulation of nanoparticles in tumor areas.Hence,it is necessary to actively control the behavior of nanoparticles when they enter the tumor microenvironment.By utilizing biocompatible and efficient click reactions,the aggregation of nanoparticles at the tumor site can be controlled,thereby enhancing nanoparticle accumulation at the target location with improved imaging signals and enhanced tumor-inhibitory effects.Herein,we introduce and classify in situ nanoparticle aggregation for biomedical imaging and therapeutic applications induced by four types of common click reactions:coppercatalyzed azide–alkyne cycloaddition(CuAAC),strain-promoted azide–alkyne cycloaddition(SPAAC),click condensation between 2-cyanobenzothiazole(CBT)and cysteine(Cys),and inverse electron-demand Diels–Alder(iEDDA).Furthermore,we summarize the main strategies of these click reaction-based nanoparticle aggregation approaches.Finally,we discuss the advantages and disadvantages of click reaction-triggered aggregation and analyze future trends.
