目的:通过检测子痫前期患者外周血中间型单核细胞HLA-DR表达并分析其与临床指标的相关性,探讨中间型单核细胞HLA-DR在子痫前期疾病过程中的作用。方法:采集22例子痫前期患者(子痫前期组)和23例健康孕妇(对照组)肘前静脉血4 m L,肝素抗...目的:通过检测子痫前期患者外周血中间型单核细胞HLA-DR表达并分析其与临床指标的相关性,探讨中间型单核细胞HLA-DR在子痫前期疾病过程中的作用。方法:采集22例子痫前期患者(子痫前期组)和23例健康孕妇(对照组)肘前静脉血4 m L,肝素抗凝。流式细胞术检测中间型单核细胞HLA-DR表达和Th17细胞阳性率;液相芯片检测血清中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、IL-17和IL-10浓度;分析子痫前期患者中间型单核细胞HLA-DR阳性频率与血清细胞因子浓度、Th17细胞阳性率的相关性。结果:子痫前期组中间型单核细胞HLA-DR阳性细胞率为(29.4±8.4)%,比对照组[(20.1±3.8)%]明显增高(P<0.01);平均荧光强度(MFI)为31.5±5.9,亦明显高于对照组(26.4±4.3,P<0.01)。与对照组比较,子痫前期患者血清中TNF-α、IL-6和IL-17浓度显著升高,IL-10浓度明显降低,差异均有统计学意义(P<0.05);子痫前期患者中间型单核细胞HLA-DR阳性细胞率与Th17细胞阳性率、血清IL-17浓度均呈正相关(r=0.468、r=0.473,P<0.05)。结论:子痫前期患者外周血中间型单核细胞HLA-DR表达明显增加,参与子痫前期的疾病过程。展开更多
Background: Studies conducted previously indicated that gradual vascular changes in diabetic retinopathy (DR) include reduction in retinal vascular density and choroidal thickness. One device that can be used to detec...Background: Studies conducted previously indicated that gradual vascular changes in diabetic retinopathy (DR) include reduction in retinal vascular density and choroidal thickness. One device that can be used to detect these early changes is optical coherence tomographic angiography (OCTA). Objective: To detect the pre and early diabetic retinal and choroidal microvascular changes in patients with type 2 diabetes mellitus using OCTA. Methods: A total of 188 eyes were included in the study that was conducted at Zhongnan Hospital of Wuhan University Ophthalmology Department. Participants were divided into three groups: controls (90 eyes), pre-DR (70 eyes) and early-DR (28 eyes). We evaluated the changes in vascular density of deep capillary plexus (DCP), intermediate capillary plexus (ICP), superficial vascular complex (SVP), choroidal thickness (CT) and choroidal vascular index (CVI). Results: Vessel density (VD) of deep capillary plexus (DCP) and intermediate capillary plexus (ICP) at 3 × 3 mm was not significant. However, superficial vascular plexus (SVP), was significant in superior quadrant, p = 0.033. In DCP layer at 6 × 6 mm, significant difference was noted superiorly, p = 0.023 and ANOVA p = 0.033. However, ICP layer showed significant changes temporaly and inferiorly in 1 - 3 mm and 1 - 6 mm retinal rings, p Conclusion: Our results reveal that although there were no clinical manifestations in the control group and the pre-DR group, changes in retinal and choroidal blood flow density had already occurred in the pre-DR group through OCTA detection, suggesting that early detection of the lesions provides an objective basis to prevent further retinal microvascular damage. Using OCTA parameters at 3 × 3 mm, 6 × 6 mm and 15 × 12 mm scan radius at different layers of the retina and choroid, we are able to detect early retinal microvascular and choroidal changes in patients with pre-DR and early-DR thereby saving the costs of dealing with advanced diabetic eye disease. OCTA therefore becomes a fundamental tool for the non-invasive diagnosis and prognosis of DR.展开更多
文摘目的:通过检测子痫前期患者外周血中间型单核细胞HLA-DR表达并分析其与临床指标的相关性,探讨中间型单核细胞HLA-DR在子痫前期疾病过程中的作用。方法:采集22例子痫前期患者(子痫前期组)和23例健康孕妇(对照组)肘前静脉血4 m L,肝素抗凝。流式细胞术检测中间型单核细胞HLA-DR表达和Th17细胞阳性率;液相芯片检测血清中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、IL-17和IL-10浓度;分析子痫前期患者中间型单核细胞HLA-DR阳性频率与血清细胞因子浓度、Th17细胞阳性率的相关性。结果:子痫前期组中间型单核细胞HLA-DR阳性细胞率为(29.4±8.4)%,比对照组[(20.1±3.8)%]明显增高(P<0.01);平均荧光强度(MFI)为31.5±5.9,亦明显高于对照组(26.4±4.3,P<0.01)。与对照组比较,子痫前期患者血清中TNF-α、IL-6和IL-17浓度显著升高,IL-10浓度明显降低,差异均有统计学意义(P<0.05);子痫前期患者中间型单核细胞HLA-DR阳性细胞率与Th17细胞阳性率、血清IL-17浓度均呈正相关(r=0.468、r=0.473,P<0.05)。结论:子痫前期患者外周血中间型单核细胞HLA-DR表达明显增加,参与子痫前期的疾病过程。
文摘Background: Studies conducted previously indicated that gradual vascular changes in diabetic retinopathy (DR) include reduction in retinal vascular density and choroidal thickness. One device that can be used to detect these early changes is optical coherence tomographic angiography (OCTA). Objective: To detect the pre and early diabetic retinal and choroidal microvascular changes in patients with type 2 diabetes mellitus using OCTA. Methods: A total of 188 eyes were included in the study that was conducted at Zhongnan Hospital of Wuhan University Ophthalmology Department. Participants were divided into three groups: controls (90 eyes), pre-DR (70 eyes) and early-DR (28 eyes). We evaluated the changes in vascular density of deep capillary plexus (DCP), intermediate capillary plexus (ICP), superficial vascular complex (SVP), choroidal thickness (CT) and choroidal vascular index (CVI). Results: Vessel density (VD) of deep capillary plexus (DCP) and intermediate capillary plexus (ICP) at 3 × 3 mm was not significant. However, superficial vascular plexus (SVP), was significant in superior quadrant, p = 0.033. In DCP layer at 6 × 6 mm, significant difference was noted superiorly, p = 0.023 and ANOVA p = 0.033. However, ICP layer showed significant changes temporaly and inferiorly in 1 - 3 mm and 1 - 6 mm retinal rings, p Conclusion: Our results reveal that although there were no clinical manifestations in the control group and the pre-DR group, changes in retinal and choroidal blood flow density had already occurred in the pre-DR group through OCTA detection, suggesting that early detection of the lesions provides an objective basis to prevent further retinal microvascular damage. Using OCTA parameters at 3 × 3 mm, 6 × 6 mm and 15 × 12 mm scan radius at different layers of the retina and choroid, we are able to detect early retinal microvascular and choroidal changes in patients with pre-DR and early-DR thereby saving the costs of dealing with advanced diabetic eye disease. OCTA therefore becomes a fundamental tool for the non-invasive diagnosis and prognosis of DR.
