While bagged flat peaches gain increasing consumer preference,the bagging process inhibits anthocyanin production and fruit coloration in most peach cultivars.Certain peach cultivars,including the rapidly expanding‘Z...While bagged flat peaches gain increasing consumer preference,the bagging process inhibits anthocyanin production and fruit coloration in most peach cultivars.Certain peach cultivars,including the rapidly expanding‘Zhongyoupan 9’,demonstrate a light-independent anthocyanin biosynthesis pattern and accumulate anthocyanins under artificial darkness.The underlying molecular mechanisms,however,remain unelucidated.This study examined two flat peach varieties:‘Zhongpan 17’,exhibiting light-dependent anthocyanin biosynthesis,and‘Zhongyoupan 9’,displaying light-independent anthocyanin biosynthesis.The pericarp of‘Zhongyoupan 9’contained substantially higher anthocyanin levels compared to‘Zhongpan 17’.Metabolomic analysis identified the marked increase in anthocyanins,particularly cyanidin-3-O-glucoside,as the primary factor in‘Zhongyoupan 9’coloration under artificial darkness.Transcriptomic analyses revealed that PpHY5(long hypocotyl 5)showed upregulation in‘Zhongyoupan 9’but downregulation in‘Zhongpan 17’,with its expression pattern correlating positively with color changes in both varieties.The role of PpHY5 in positively regulating anthocyanin biosynthesis was confirmed through reduced anthocyanin levels in peach fruits treated with a PpHY5 virus-induced gene-silencing construct.These findings suggest that the PpHY5 regulates red color development in‘Zhongyoupan 9’under artificial darkness.展开更多
基金supported by the National Natural Science Foundation of China(32402523)the Science and Technology Program of Henan Province,China(232102111083,242102111120)the Natural Science Foundation of Henan Province,China(252300420179).
文摘While bagged flat peaches gain increasing consumer preference,the bagging process inhibits anthocyanin production and fruit coloration in most peach cultivars.Certain peach cultivars,including the rapidly expanding‘Zhongyoupan 9’,demonstrate a light-independent anthocyanin biosynthesis pattern and accumulate anthocyanins under artificial darkness.The underlying molecular mechanisms,however,remain unelucidated.This study examined two flat peach varieties:‘Zhongpan 17’,exhibiting light-dependent anthocyanin biosynthesis,and‘Zhongyoupan 9’,displaying light-independent anthocyanin biosynthesis.The pericarp of‘Zhongyoupan 9’contained substantially higher anthocyanin levels compared to‘Zhongpan 17’.Metabolomic analysis identified the marked increase in anthocyanins,particularly cyanidin-3-O-glucoside,as the primary factor in‘Zhongyoupan 9’coloration under artificial darkness.Transcriptomic analyses revealed that PpHY5(long hypocotyl 5)showed upregulation in‘Zhongyoupan 9’but downregulation in‘Zhongpan 17’,with its expression pattern correlating positively with color changes in both varieties.The role of PpHY5 in positively regulating anthocyanin biosynthesis was confirmed through reduced anthocyanin levels in peach fruits treated with a PpHY5 virus-induced gene-silencing construct.These findings suggest that the PpHY5 regulates red color development in‘Zhongyoupan 9’under artificial darkness.
