Objective:To investigate Pothos scandens for the in vitro antioxidant and antipyretic activity. Methods:Preliminary phylochemicals,total phenolics and flavonoid contents were analyzed in leaf,stem and root samples.In ...Objective:To investigate Pothos scandens for the in vitro antioxidant and antipyretic activity. Methods:Preliminary phylochemicals,total phenolics and flavonoid contents were analyzed in leaf,stem and root samples.In vitro antioxidant activity was evaluated by different assays such as 2,2-diphenyl-1-picrylhydrazyl（DPPH） radical scavenging,2,2’-azinobis -3-ethylbenzolhiazoline-6-sulphonic acid（ABTS<sup>+</sup>） radical scavenging,ferric-reducing antioxidant power（FRAP） assay,phosphomolybdenum reduction assay,metal chelating activity, superoxide anion radical scavenging activity,hydrogen peroxide and nitric oxide scavenging assay.The antipyretic activity of root methanol extract was studied by pyrexia induced by brewer’s yeast on Wistar albino rats at concentration of 200 and 400 mg/kg using paracetamol as standard drug.Results:The total phenolics and tannin content were found to be higher in elhanol extract of stem,whereas total flavonoid content was higher in acetone extract of root.The methanol extract of root showed highest free radical scavenging activity in assays namely ABTS assay（8 221.5μM TE/g extract）,FRAP assay[514.4 mM Fe（Ⅱ）/g extract],hydrogen peroxide （60.3%） and nitric oxide scavenging assays（58.7%）.The DPPH assay and superoxide radical assay results revealed that the ethanol extract of root has remarkable free radical scavenging capacity(IC<sub>50</sub> 0.284 mg/mL and 70.84%).The antipyretic studies on methanol extract of root showed significant reduction of temperature in pyrexia induced rats at 200 and 400 mg/kg doses. Conclusions:These findings justify that Pothos scan/km can be a valuable natural antioxidant and antipyretic source which seemed to provide potential nutraccuticals for human health.展开更多
Objective:To formulate Pothos scandens Linn(P.scandens) leaf extract in to a gel and investigate their burn wound healing activity.Methods:Ethanolic extract of dried leaves of P. scandens were subjected to priliminery...Objective:To formulate Pothos scandens Linn(P.scandens) leaf extract in to a gel and investigate their burn wound healing activity.Methods:Ethanolic extract of dried leaves of P. scandens were subjected to priliminery phytochemical evaluation and wound healing activities studies.Different gel formulations of ethanolic extract of P.scandens(4%w/v) were prepared using polymers carbopol 934 and carbopol 940 bv varying their concentration.These formulations were evaluated for physical parameters,drug content.pH,viscosity,extrudability.spreadability. primary skin irritation,pharmacological activity and stability.Results:Wound healing studies of ethanolic extract revealed that P.scandens treated animals were found to epithelise in 22 days while the solvent control and the untreated rats epithelise within 35 and 40 days respectively. The formulation with 1.5%w/w carbopol 934 was found to be more promising as it shows better physicochemical characteristics,higher pharmacological activity and stability compared to other formulations.Conclusions:P.scandens alcoholic extract shows significant improvement in burn wound contraction and hence this is a promising candidate in burn wound healing.展开更多
文摘Objective:To investigate Pothos scandens for the in vitro antioxidant and antipyretic activity. Methods:Preliminary phylochemicals,total phenolics and flavonoid contents were analyzed in leaf,stem and root samples.In vitro antioxidant activity was evaluated by different assays such as 2,2-diphenyl-1-picrylhydrazyl（DPPH） radical scavenging,2,2’-azinobis -3-ethylbenzolhiazoline-6-sulphonic acid（ABTS<sup>+</sup>） radical scavenging,ferric-reducing antioxidant power（FRAP） assay,phosphomolybdenum reduction assay,metal chelating activity, superoxide anion radical scavenging activity,hydrogen peroxide and nitric oxide scavenging assay.The antipyretic activity of root methanol extract was studied by pyrexia induced by brewer’s yeast on Wistar albino rats at concentration of 200 and 400 mg/kg using paracetamol as standard drug.Results:The total phenolics and tannin content were found to be higher in elhanol extract of stem,whereas total flavonoid content was higher in acetone extract of root.The methanol extract of root showed highest free radical scavenging activity in assays namely ABTS assay（8 221.5μM TE/g extract）,FRAP assay[514.4 mM Fe（Ⅱ）/g extract],hydrogen peroxide （60.3%） and nitric oxide scavenging assays（58.7%）.The DPPH assay and superoxide radical assay results revealed that the ethanol extract of root has remarkable free radical scavenging capacity(IC<sub>50</sub> 0.284 mg/mL and 70.84%).The antipyretic studies on methanol extract of root showed significant reduction of temperature in pyrexia induced rats at 200 and 400 mg/kg doses. Conclusions:These findings justify that Pothos scan/km can be a valuable natural antioxidant and antipyretic source which seemed to provide potential nutraccuticals for human health.
文摘Objective:To formulate Pothos scandens Linn(P.scandens) leaf extract in to a gel and investigate their burn wound healing activity.Methods:Ethanolic extract of dried leaves of P. scandens were subjected to priliminery phytochemical evaluation and wound healing activities studies.Different gel formulations of ethanolic extract of P.scandens(4%w/v) were prepared using polymers carbopol 934 and carbopol 940 bv varying their concentration.These formulations were evaluated for physical parameters,drug content.pH,viscosity,extrudability.spreadability. primary skin irritation,pharmacological activity and stability.Results:Wound healing studies of ethanolic extract revealed that P.scandens treated animals were found to epithelise in 22 days while the solvent control and the untreated rats epithelise within 35 and 40 days respectively. The formulation with 1.5%w/w carbopol 934 was found to be more promising as it shows better physicochemical characteristics,higher pharmacological activity and stability compared to other formulations.Conclusions:P.scandens alcoholic extract shows significant improvement in burn wound contraction and hence this is a promising candidate in burn wound healing.
