The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and contin...The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.展开更多
Objective:The aim of the study is to evaluate the effect of neck massage on increased breast milk secretion among postnatal mothers.Materials and Methods:The current study adopted a quasi-experimental design conducted...Objective:The aim of the study is to evaluate the effect of neck massage on increased breast milk secretion among postnatal mothers.Materials and Methods:The current study adopted a quasi-experimental design conducted among 60 primi postnatal mothers(30 in each group).Study was conducted at the Postnatal ward of IMS and SUM Hospital,Bhubaneswar,Odisha.Tools used were structured sociodemographic pro forma,structured breast milk adequacy questionnaire,and structured rating scale to assess the adequacy of breast milk production.The duration of the study is 1 year.All the postnatal mothers who were primigravida and undergone cesarean section were chosen as the sample through a convenience sampling technique.Then,the samples were assigned to the intervention group(receiving neck massage)and control group(receiving standard postnatal care).The data were collected and analyzed using both descriptive and inferential statistics by SPSS version 20.Results:The study resulted a significant difference between the pretest and posttest score of breast milk production after neck massage(P=0.000).The mean score of the posttest(2.90±0.36)is more than the mean score of the pretest(2.89±0.36)of the baby’s weight(P<0.001).Further,the number of urination and stool passed in 24 h was more in the intervention group than the control group(P<0.001).The Chi-square analysis showed statistical significance between breast milk production after neck massage with religion of the postnatal mothers(P<0.05).Conclusion:The neck massage is found effective in improving the breast milk production among primi c-section mothers with improve newborn parameters.展开更多
Dear Editors,The term“developmental disconnection syndromes”(DDSs)was first coined by Geshwind and Levitt in 2007[1]to describe the weakening of already formed connections or an absence of certain connections to est...Dear Editors,The term“developmental disconnection syndromes”(DDSs)was first coined by Geshwind and Levitt in 2007[1]to describe the weakening of already formed connections or an absence of certain connections to establish correct organization de novo in early developmental stages.DDSs include a number of neuropsychiatric diseases,such as autistic spectrum disorder(ASD)[1,2],which is characterized by impaired social communication and repetitive and stereotyped behaviors.Nevertheless,the exact etiology underlying these“disconnections”and their abnormal developmental trajectory remains largely unclear.Even less known is whether developmental disconnections relevant to autism can be rescued by early intervention thereby preventing neuropsychiatric and repetitive symptoms in later stages.This study addresses these important questions.展开更多
Background:Postcesarean mothers often experience delayed lactogenesis II due to surgical stress and reduced oxytocin levels.Almond oil breast massage is a nonpharmacological intervention thought to enhance prolactin r...Background:Postcesarean mothers often experience delayed lactogenesis II due to surgical stress and reduced oxytocin levels.Almond oil breast massage is a nonpharmacological intervention thought to enhance prolactin release and improve milk production.Objective:The objective of the study was to evaluate the effectiveness of almond oil massage on breast milk adequacy among primigravida lower segment cesarean section(LSCS)mothers.Materials and Methods:A randomized pretest-posttest control group design was conducted among 60 primigravida mothers post-LSCS,randomly assigned to experimental(n=30)and control(n=30)groups.The experimental group received three daily almond oil breast massages(5-10 mL;5 min/breast)for 3 days;controls received routine care.Breast milk adequacy was measured using a validated 16-item rating scale at baseline and day 4 post-intervention.Results:The mean breast milk adequacy scores increased from 29.30±1.915 to 39.80±1.690 in the experimental group(t=22.15,P<0.001).Control group change(29.93±2.132-30.27±2.116)was non-significant(t=0.776,P>0.05).Posttest scores between the two groups differed significantly between groups(t=19.282,P<0.001).Conclusion:Almond oil breast massage significantly enhances breast milk adequacy in post-LSCS mothers and can be integrated into routine postnatal nursing care.展开更多
The oxytocin receptor(OXTR)has garnered increasing attention for its role in regulating both mature behaviors and brain development.It has been established that OXTR mediates a range of effects that are regionspecifc ...The oxytocin receptor(OXTR)has garnered increasing attention for its role in regulating both mature behaviors and brain development.It has been established that OXTR mediates a range of effects that are regionspecifc or period-specifc.However,the current studies of OXTR expression patterns in mice only provide limited help due to limitations in resolution.Therefore,our objective was to generate a comprehensive,high-resolution spatiotemporal expression map of Oxtr mRNA across the entire developing mouse brain.We applied RNAscope in situ hybridization to investigate the spatiotemporal expression pattern of Oxtr in the brains of male mice at six distinct postnatal developmental stages(P7,P14,P21,P28,P42,P56).We provide detailed descriptions of Oxtr expression patterns in key brain regions,including the cortex,basal forebrain,hippocampus,and amygdaloid complex,with a focus on the precise localization of Oxtr+cells and the variance of expression between diferent neurons.Furthermore,we identifed some neuronal populations with high Oxtr expression levels that have been little studied,including glutamatergic neurons in the ventral dentate gyrus,Vgat+Oxtr+cells in the basal forebrain,and GABAergic neurons in layers 4/5 of the cortex.Our study provides a novel perspective for understanding the distribution of Oxtr and encourages further investigations into its functions.展开更多
In the present study,the effects of postnatal development on the number and distribution of skeletal muscle fibers of different types in hind leg of rat and rabbit were studied.The soleus muscles of rats aged 2 days,2...In the present study,the effects of postnatal development on the number and distribution of skeletal muscle fibers of different types in hind leg of rat and rabbit were studied.The soleus muscles of rats aged 2 days,2,4,6,8,and 10 weeks (body weight 10,32,95,190,280,and 320 g),and rabbits aged 2 days,2,4,8,12,16,20,and 24 weeks (body weight 100,220,400,750,1 200,1 600,2 100,and 2 500 g) were stained with succinic dehydrogenase.With an image analysis system,the X-Y coordinates of fibers were used to analyze the growth-related changes.The results of present study showed that three types of fibers were found in the soleus muscles of rat and rabbit,i.e.,type Ⅰ (slow oxidative),ⅡX (fast oxidative),and ⅡA (fast oxidative glycolytic).The type Ⅰ fibers were present throughout the muscle that had a uniform distribution and tended to increase in number with aging.Type ⅡX fibers were scattered throughout the muscle and decreased markedly in number with aging.Type ⅡA fibers were located at the central and deep regions,and showed a little or no change in number and distribution with aging.While be of age,type ⅡA and ⅡX fibers became restricted to the superficial region.No type ⅡB fibers were detected.Type ⅡA fibers had the largest diameter,type Ⅰ intermediate and type ⅡX the smallest.Mean cross-sectional area of each type fibers of rabbits was larger than that of rats.The present results indicate that the number and distribution of muscle fibers of different types in hind limb of rat and rabbit change with the process of postnatal growth.展开更多
Our previous studies showed that the early use of calpain inhibitors reduces calpain activity in multiple brain regions, and that postnatal treatment with calpeptin may lead to cerebellar motor dysfunction. However, i...Our previous studies showed that the early use of calpain inhibitors reduces calpain activity in multiple brain regions, and that postnatal treatment with calpeptin may lead to cerebellar motor dysfunction. However, it remains unclear whether postnatal calpeptin application affects hippocampus-related behaviors. In this study, Sprague-Dawley rats were purchased from the Animal Center of Anhui Medical University of China. For the experiments in the adult stage, rats were intraperitoneally injected with calpeptin, 2 mg/kg, once a day, on postnatal days 7–14. Then on postnatal day 60, the Morris water maze test was used to evaluate spatial learning and memory abilities. The open field test was carried out to assess anxiety-like activities. Phalloidin staining was performed to observe synaptic morphology in the hippocampus. Immunohistochemistry was used to count the number of NeuN-positive cells in the hippocampal CA1 region. DiI was applied to label dendritic spines. Calpeptin administration impaired spatial memory, caused anxiety-like behavior in adulthood, reduced the number and area of apical dendritic spines, and decreased actin polymerization in the hippocampus, but did not affect the number of NeuN-positive cells in the hippocampal CA1 region. For the neonatal experiments, neonatal rats were intraperitoneally injected with calpeptin, 2 mg/kg, on postnatal days 7 and 8. Western blot assay was performed to analyze the protein levels of Akt, Erk, p-Akt, p-Erk1/2, Erk1/2, SCOP, PTEN, mTOR, p-mTOR, CREB and p-CREB in the hippocampus. SCOP expression was increased, and the phosphorylation levels of Akt, mTOR and CREB were reduced in the hippocampus. These findings show that calpeptin administration after birth affects synaptic development in neonatal rats by inhibiting the Akt/mTOR signaling pathway, thereby perturbing hippocampal function. Therefore, calpeptin administration after birth is a risk factor for neurodevelopmental defects.展开更多
Aim： To immunolocalize the c-mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development. Methods： Semi-quantitative reverse transcription-polymerase chain re...Aim： To immunolocalize the c-mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development. Methods： Semi-quantitative reverse transcription-polymerase chain reaction （RT- PCR） and in situ hybridization techniques were used to examine c-mos mRNA and indirect immunofluorescence was used to localize c-Mos protein in mouse testis on postnatal days 14, 21, 25, 28, 30, 35, 49 and 70. Results： c-mos mRNA remained low on postnatal days 14-21, increased abruptly from day 25 and peaked on day 30. Its levels decreased a little on day 35 and became almost stable thereafter until day 70. c-mos mRNA was localized in the nucleus and cytoplasm of the spermatocytes and round spermatids. The nuclear staining was much stronger than the cytoplasmic staining. Using a polyclonal anti-c-Mos antibody, Western blotting detected a single band at 43 kDa in testis lysate, c-Mos protein was exclusively localized to the elongating spermatids and was first detected on postnatal day 30. The number of c-Mos-positive spermatids increased progressively till day 49 and stabilized thereafter. Conclusion： The c-mos gene displays a spatial and temporal expression pattern in the mouse testis during postnatal development at both the mRNA and protein level. This suggests that c-mos might play important roles in spermatogenesis. （Asian J Androl 2008 Mar; 10： 277-285）展开更多
The study aimed to determine whether antenatal fear of childbirth (expectation) could predict postnatal fear of childbirth (experience) by taking account of other antenatal psychological variables (anxiety and depress...The study aimed to determine whether antenatal fear of childbirth (expectation) could predict postnatal fear of childbirth (experience) by taking account of other antenatal psychological variables (anxiety and depression) as well as birth outcomes in Japanese women. A longitudinal observational study was conducted at a clinic in Tokyo, Japan, in 2011. Self-report questionnaires were distributed to 240 Japanese women at 37 gestational weeks (Time 1) and on the second day after delivery (Time 2). Regression analyses by means of structural equation modelling were conducted in both the primiparous and the multiparous group. The models exhibited good fit (chisquare value/degree of freedom = 1.10 - 1.62, comparative fit index = 0.92 - 0.99 and root mean square error of approximation = 0.03 - 0.07). Antenatal fear of childbirth was the most predictive variable of postnatal fear of childbirth in both the primiparous (β = 0.58, p = 0.002) and the multiparous group (β = 0.62, p < 0.001). In conclusion, antenatal fear of childbirth was a significant predictor of postnatal fear of childbirth when other antenatal psychological variables and birth outcomes were taken into account. Pregnant women who are strongly afraid of childbirth need special attention before and after delivery.展开更多
Background: RNA editing is a widespread post-transcriptional modification mechanism in mammalian genomes.Although many editing sites have been identified in domestic pigs(Sus scrofa), little is known about the charact...Background: RNA editing is a widespread post-transcriptional modification mechanism in mammalian genomes.Although many editing sites have been identified in domestic pigs(Sus scrofa), little is known about the characteristics and dynamic regulation of RNA editing in the pineal gland(PG), a small neuroendocrine gland that synthesizes and secretes melatonin, which is primarily responsible to modulate sleep patterns.Results: This study analyzed the expression of adenosine-to-inosine(A-to-I) editing regulators and profiled the first dynamic A-to-I RNA editome during postnatal PG development. The results identified ADAR1 as the most abundantly expressed ADAR enzyme, which was down-regulated during postnatal PG development. Furthermore,47,284 high-confidence RNA editing sites were identified, the majority of which(93.6%) were of the canonical A-to-I editing type, followed by C-to-T editing. Analysis of its characteristics showed that the A-to-I editing sites mostly localized in SINE retrotransposons PRE-1/Pre0_SS. Moreover, a strong deficiency and preference for guanine nucleotides at positions of one base upstream or downstream were found, respectively. The overall editing level at the puberty stage was higher than at both infancy and adulthood stages. Additionally, genome-wide RNA editing was found to exhibit a dynamic stage-specific fashion(postnatally). Genes that underwent developmental changes in RNA editing were associated with catabolic processes as well as protein localization and transport functions,implying that RNA editing might be responsible for the molecular machineries of the postnatal developing PG.Remarkably, RNA editing in 3′-UTRs might regulate gene expression by influencing miRNA binding during PG development.Conclusions: This study profiles the first comprehensive developmental RNA editome in the pig PG, which contributes to the understanding of the importance of post-transcriptionally mediated regulation during mammalian postnatal PG development. Moreover, this study widely extends RNA editome resources in mammals.展开更多
To investigate the expression pattern of rat Eppin （epididymal protease inhibitor; official symbol Spinlwl）, we detected mRNA transcripts and subsequent protein translation of Eppin in several sorts of tissues by RT...To investigate the expression pattern of rat Eppin （epididymal protease inhibitor; official symbol Spinlwl）, we detected mRNA transcripts and subsequent protein translation of Eppin in several sorts of tissues by RT-PCR and westem blotting. Then immunohistochemistry was performed for more detailed observation. The testicular transcription level was monitored by real-time PCR throughout postnatal development. We found that rat Eppin was specifically expressed in the testis and epididymis. The testicular transcription was slight in neonatal （1-day） and infantile stages （5-, 7- and 10-day）. It increased sharply thereafter, with maximum expression level （about 38- fold compared with that of 1-day old rat） detected in prepubertal stage （15-day）. Then a slightly declined but stable level （about 20-fold compared with that of 1-day old rat） was kept in pubertal-early adult （30-day） and adult （60-day） stages of postnatal maturation. In the adult rat, EPPIN protein was mainly localized in the elongated spermatids and epididymal epithelial cells. Sperm in the epididymal duct were all covered with EPPIN and its level kept constant during incubation under conditions used to achieve capacitation. Its stage-specific expression in the testis suggests that EPPIN may be important during spermatogenesis especially for the spermatid elongation. The abundant production of epididymal EPPIN indicated indirectly that it might play a role in the function of the epididymis.展开更多
A large body of literature suggests that bone metabolism is susceptible to the ill effects of reactive species that accumulate in the body and cause cellular dysfunction. One of the body＇s front lines in defense agai...A large body of literature suggests that bone metabolism is susceptible to the ill effects of reactive species that accumulate in the body and cause cellular dysfunction. One of the body＇s front lines in defense against such damage is the transcription factor, Nrf2. This transcription factor regulates a plethora of antioxidant and cellular defense pathways to protect cells from such damage. Despite the breadth of knowledge of both the function of Nrf2 and the effects of reactive species in bone metabolism, the direct role of Nrf2 in skeletal biology has yet to be thoroughly examined. Thus, in the current study, we have examined the role of Nrf2 in postnatal bone metabolism in mice. Mice lacking Nrf2 （Nrf2-/-） exhibited a marked deficit in postnatal bone acquisition, which was most severe at 3 weeks of age when osteoblast numbers were 12-fold less than observed in control animals. While primary osteoblasts from Nrf2-/- mice functioned normally in vitro, the colony forming capacity of bone marrow stromal cells （BMSCs） from these mice was significantly reduced compared to controls. This defect could be rescued through treatment with the radical scavenger N-acetyl cysteine （NAC）, suggesting that increased reactive species stress might impair early osteoblastogenesis in BMSCs and lead to the failure of bone acquisition observed in Nrf2-/- animals. Taken together, these studies suggest Nrf2 represents a key pathway in regulating bone metabolism, which may provide future therapeutic targets to treat osteoporosis.展开更多
Nitric oxide is an important neuromodulator in the brain and is involved in the development of visual system. But it is not clear how nitric oxide and nitric oxide synthase （NOS） are involved in the developing visua...Nitric oxide is an important neuromodulator in the brain and is involved in the development of visual system. But it is not clear how nitric oxide and nitric oxide synthase （NOS） are involved in the developing visual cortex of rodents. Thus we examined the expression of NOS activity in the postnatal developing visual cortex of the golden hamster by using histochemical technique for NADPH-diaphorase （NADPH-d）. A heavily stained NADPH-d band was observed in the neuropil of the visual cortex. This NADPH-d band initially appeared in the cortical plate from the day of birth （P0） to postnatal day 4 （P4）. From P7 to P21, this band was confined to area 17 and migrated to the deeper layers Ill IV and V VI before it eventually disappeared at P28. Such developmental trends of the band correlated well with the process of formation and establishment of the geniculo-cortical projection patterns. Thus, the areal specific development of the band suggests that NOS is closely related to the cortical differentiation and synaptic formation of the primary visual cortex. On the other hand, monocular eye enucleation on P1 could not alter the appearance of this NADPH-d positive band, indicating a non-activity dependant role of NOS. In addition, differences in the laminar distributions and developmental sequence between the heavily and lightly stained NADPH-d positive neurons during development suggest that they play different roles in the development.展开更多
Fetal eutaneous wounds that oeeur in earlygestation heal without sear formation.Althoughmueh work has been done to eharaeterize the roleof transforming growth
Background: Negative experiences in early life can induce long-lasting effects on the welfare, health, and performance of farm animals. A delayed placement of chicks in rearing houses has negative effects on their per...Background: Negative experiences in early life can induce long-lasting effects on the welfare, health, and performance of farm animals. A delayed placement of chicks in rearing houses has negative effects on their performance, and results in fecal-specific odors detectable by rats. Based on this observation, the volatile organic compounds(VOCs) and metabolites from the feces of 12-day-old chickens were screened for early markers of response to negative events using gas-chromatography and liquid-chromatography coupled with mass spectrometry(GC-MS, LC-HRMS).Results: The low reproducibility of solid-phase micro-extraction of the VOCs followed by GC-MS was not suitable for marker discovery, in contrast to liquid extraction of metabolites from freeze-dried feces followed by GC-MS or LC-HRMS analysis. Therefore, the fecal metabolome from 12-day-old chicks having experienced a normal or delayed placement were recorded by GC-MS and LC-HRMS in two genotypes from two experiments. From both experiments, 25 and 35 metabolites, respectively explaining 81% and 45% of the difference between delayed and control chickens, were identified by orthogonal partial least-squares discriminant analysis from LC-HRMS and GC-MS profiling.Conclusion: The sets of molecules identified will be useful to better understand the chicks’ response to negative events over time and will contribute to define stress or welfare biomarkers.展开更多
AIM: To suggest a novel retinopathy of prematurity(ROP) screening model in developing countries incorporating postnatal weight gain ratios(PWGR) to traditional parameters to maintain sensitivity and improve specificit...AIM: To suggest a novel retinopathy of prematurity(ROP) screening model in developing countries incorporating postnatal weight gain ratios(PWGR) to traditional parameters to maintain sensitivity and improve specificity in detecting ROP. METHODS: Analysis of weekly PWGR of infants from one tertiary referral center during six months to determine the age at which the PWGR with the highest predictability for ROP development which was referred to as the postnatal net weight gain ratio(NWGR). NWGR was added to conventional criteria to describe a new model(The Alex-ROP model). RESULTS: Of 560 infants were included. NWGR 28 d after birth was the most predictive factor for the development of ROP. A new model Alex-ROP recommending screening infants with gestational age(GA) ≤33 wk or birth weight ≤1500 g and NWGR at 28 d after birth <0.3 was suggested. A second screening model referred to as High-grade AlexROP(Hg Alex-ROP) model to detect worse grade ROP(Both type 1 and type 2) recommending a cutoff point of NWGR <0.15 between birth and 28^(th) day. CONCLUSION: Both Alex-ROP and Hg Alex-ROP models are easy to apply to improve the specificity of ROP screening in developing countries while maintaining high sensitivity.展开更多
To define the function of Zbtb20 in vivo,we generated knockout mice by homologous recombination.Zbtb20 null mice displayed a stark phenotype characterized by postnatal growth retardation,
The visual system is affected by neurodegenerative diseases caused by the degeneration of specific retinal neurons,the leading cause of irreversible blindness in humans.Throughout vertebrate phylogeny,the retina has t...The visual system is affected by neurodegenerative diseases caused by the degeneration of specific retinal neurons,the leading cause of irreversible blindness in humans.Throughout vertebrate phylogeny,the retina has two kinds of specialized niches of constitutive neurogenesis:the retinal progenitors located in the circumferential marginal zone and Müller glia.The proliferative activity in the retinal progenitors located in the circumferential marginal zone in precocial birds such as the chicken,the commonest bird model used in developmental and regenerative studies,is very low.This region adds only a few retinal cells to the peripheral edge of the retina during several months after hatching,but does not seem to be involved in retinal regeneration.Müller cells in the chicken retina are not proliferative under physiological conditions,but after acute damage some of them undergo a reprogramming event,dedifferentiating into retinal stem cells and generating new retinal neurons.Therefore,regenerative response after injury occurs with low efficiency in the precocial avian retina.In contrast,it has recently been shown that neurogenesis is intense in the retina of altricial birds at hatching.In particular,abundant proliferative activity is detected both in the circumferential marginal zone and in the outer half of the inner nuclear layer.Therefore,stem cell niches are very active in the retina of altricial birds.Although more extensive research is needed to assess the potential of proliferating cells in the adult retina of altricial birds,it emerges as an attractive model for studying different aspects of neurogenesis and neural regeneration in vertebrates.展开更多
Environmental threats often trigger innate defensive responses in mammals.However,the gradual development of functional properties of these responses during the postnatal development stage remains unclear.Here,we repo...Environmental threats often trigger innate defensive responses in mammals.However,the gradual development of functional properties of these responses during the postnatal development stage remains unclear.Here,we report that looming stimulation in mice evoked fight behavior commencing at P14-16 and had fully developed by P20-24.The visual-evoked innate defensive response was not significantly altered by sensory deprivation at an early postnatal stage.Furthermore,the percentages of wide-field and horizontal cells in the superior colliculus were notably elevated at P20-24.Our findings define a developmental time window for the formation of the visual innate defense response during the early postnatal period and_provide important insight into the underlying mechanism.展开更多
Aim: This randomize controlled experimental study has been carried out to investigate the effects on the integrity of perine of perineal massage and Kegel exercises applied prenatally to women who experienced vaginal ...Aim: This randomize controlled experimental study has been carried out to investigate the effects on the integrity of perine of perineal massage and Kegel exercises applied prenatally to women who experienced vaginal delivery. Methods: Research was carried out between January 2012 and 2013, with a total of 101 pregnant women who referred to Ege University Hospital in ?zmir. Data Collection Form, Kegel Exercise Training Brochure, Practice Observation Form and Prenatal Perinea Massage Learning Guide for Implementer were used. Researcher continued to perform this massage once a week until delivery. Kegel exercises were asked to perform exercises at home and also to register them until delivery. When exercise group came to weekly controls or when they were contacted at home they were asked if they have performed daily exercise or not. The pregnant women in control group did not receive any application. One to one interview was performed during delivery and postnatal 24 hours at the hospital and a telephone interview was performed 15 days postnatal, so three groups were evaluated. The chi-square, Mann-Whitney U test and Kruskal Wallis test were used. Results: A statistically significant difference was found between study and control groups in terms of episiotomy rates, laceration, postnatal 24 hours and 15 days perineal pain and improvement (p < 0.05). Conclusion: It was found that perineal massage and Kegel exercises are important in maintaining the integrity of perineal significantly. It is thought that when the perineal massage and Kegel exercise being performed during pregnancy is supported by health professional, it will play a significant role in women’s quality of life.展开更多
基金supported by the National Natural Science Foundation of China,Nos.81901156(to ZZ),82271200(to ZZ),82171308(to XC)the Fundamental Research Funds for the Central Universities,No.xzy012022035(to ZZ)+1 种基金the Natural Science Foundation of Shaanxi Province,Nos.2021JM-261(to QK),2023-YBSF-303(to ZZ)Traditional Chinese Medicine Project of Shaanxi Province,No.2019-ZZ-JC047(to QK)。
文摘The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.
文摘Objective:The aim of the study is to evaluate the effect of neck massage on increased breast milk secretion among postnatal mothers.Materials and Methods:The current study adopted a quasi-experimental design conducted among 60 primi postnatal mothers(30 in each group).Study was conducted at the Postnatal ward of IMS and SUM Hospital,Bhubaneswar,Odisha.Tools used were structured sociodemographic pro forma,structured breast milk adequacy questionnaire,and structured rating scale to assess the adequacy of breast milk production.The duration of the study is 1 year.All the postnatal mothers who were primigravida and undergone cesarean section were chosen as the sample through a convenience sampling technique.Then,the samples were assigned to the intervention group(receiving neck massage)and control group(receiving standard postnatal care).The data were collected and analyzed using both descriptive and inferential statistics by SPSS version 20.Results:The study resulted a significant difference between the pretest and posttest score of breast milk production after neck massage(P=0.000).The mean score of the posttest(2.90±0.36)is more than the mean score of the pretest(2.89±0.36)of the baby’s weight(P<0.001).Further,the number of urination and stool passed in 24 h was more in the intervention group than the control group(P<0.001).The Chi-square analysis showed statistical significance between breast milk production after neck massage with religion of the postnatal mothers(P<0.05).Conclusion:The neck massage is found effective in improving the breast milk production among primi c-section mothers with improve newborn parameters.
基金support JCS,QX,and BQ,as well as covering all experimental and material costs for this project,NIH/NIMH R01 MH094360-06(HWD)grant to support HX,MZ,Lei Gao,Lin Gou,HH and HWD.
文摘Dear Editors,The term“developmental disconnection syndromes”(DDSs)was first coined by Geshwind and Levitt in 2007[1]to describe the weakening of already formed connections or an absence of certain connections to establish correct organization de novo in early developmental stages.DDSs include a number of neuropsychiatric diseases,such as autistic spectrum disorder(ASD)[1,2],which is characterized by impaired social communication and repetitive and stereotyped behaviors.Nevertheless,the exact etiology underlying these“disconnections”and their abnormal developmental trajectory remains largely unclear.Even less known is whether developmental disconnections relevant to autism can be rescued by early intervention thereby preventing neuropsychiatric and repetitive symptoms in later stages.This study addresses these important questions.
基金funded by the Sum Nursing College,SOA University,Bhubaneswar,Odisha.
文摘Background:Postcesarean mothers often experience delayed lactogenesis II due to surgical stress and reduced oxytocin levels.Almond oil breast massage is a nonpharmacological intervention thought to enhance prolactin release and improve milk production.Objective:The objective of the study was to evaluate the effectiveness of almond oil massage on breast milk adequacy among primigravida lower segment cesarean section(LSCS)mothers.Materials and Methods:A randomized pretest-posttest control group design was conducted among 60 primigravida mothers post-LSCS,randomly assigned to experimental(n=30)and control(n=30)groups.The experimental group received three daily almond oil breast massages(5-10 mL;5 min/breast)for 3 days;controls received routine care.Breast milk adequacy was measured using a validated 16-item rating scale at baseline and day 4 post-intervention.Results:The mean breast milk adequacy scores increased from 29.30±1.915 to 39.80±1.690 in the experimental group(t=22.15,P<0.001).Control group change(29.93±2.132-30.27±2.116)was non-significant(t=0.776,P>0.05).Posttest scores between the two groups differed significantly between groups(t=19.282,P<0.001).Conclusion:Almond oil breast massage significantly enhances breast milk adequacy in post-LSCS mothers and can be integrated into routine postnatal nursing care.
基金supported by the National Science Fund for Distinguished Young Scholars(32325025)the STI2030-Major Projects(2021ZD0202500)+2 种基金the National Key Research and Development Program of China(2021YFA1101801)the National Natural Science Foundation of China(32171148,and 31770929)the Beijing Municipal Science and Technology Commission(Z181100001518001 and Z161100000216154).
文摘The oxytocin receptor(OXTR)has garnered increasing attention for its role in regulating both mature behaviors and brain development.It has been established that OXTR mediates a range of effects that are regionspecifc or period-specifc.However,the current studies of OXTR expression patterns in mice only provide limited help due to limitations in resolution.Therefore,our objective was to generate a comprehensive,high-resolution spatiotemporal expression map of Oxtr mRNA across the entire developing mouse brain.We applied RNAscope in situ hybridization to investigate the spatiotemporal expression pattern of Oxtr in the brains of male mice at six distinct postnatal developmental stages(P7,P14,P21,P28,P42,P56).We provide detailed descriptions of Oxtr expression patterns in key brain regions,including the cortex,basal forebrain,hippocampus,and amygdaloid complex,with a focus on the precise localization of Oxtr+cells and the variance of expression between diferent neurons.Furthermore,we identifed some neuronal populations with high Oxtr expression levels that have been little studied,including glutamatergic neurons in the ventral dentate gyrus,Vgat+Oxtr+cells in the basal forebrain,and GABAergic neurons in layers 4/5 of the cortex.Our study provides a novel perspective for understanding the distribution of Oxtr and encourages further investigations into its functions.
文摘In the present study,the effects of postnatal development on the number and distribution of skeletal muscle fibers of different types in hind leg of rat and rabbit were studied.The soleus muscles of rats aged 2 days,2,4,6,8,and 10 weeks (body weight 10,32,95,190,280,and 320 g),and rabbits aged 2 days,2,4,8,12,16,20,and 24 weeks (body weight 100,220,400,750,1 200,1 600,2 100,and 2 500 g) were stained with succinic dehydrogenase.With an image analysis system,the X-Y coordinates of fibers were used to analyze the growth-related changes.The results of present study showed that three types of fibers were found in the soleus muscles of rat and rabbit,i.e.,type Ⅰ (slow oxidative),ⅡX (fast oxidative),and ⅡA (fast oxidative glycolytic).The type Ⅰ fibers were present throughout the muscle that had a uniform distribution and tended to increase in number with aging.Type ⅡX fibers were scattered throughout the muscle and decreased markedly in number with aging.Type ⅡA fibers were located at the central and deep regions,and showed a little or no change in number and distribution with aging.While be of age,type ⅡA and ⅡX fibers became restricted to the superficial region.No type ⅡB fibers were detected.Type ⅡA fibers had the largest diameter,type Ⅰ intermediate and type ⅡX the smallest.Mean cross-sectional area of each type fibers of rabbits was larger than that of rats.The present results indicate that the number and distribution of muscle fibers of different types in hind limb of rat and rabbit change with the process of postnatal growth.
基金supported by the National Natural Science Foundation of China,No.81601181,81673716(to GZ)the Natural Science Foundation of Anhui Province of China,No.1808085J15(to GZ)the Natural Science Research Project of Anhui Province of China,No.KJ2016A417(to GZ)
文摘Our previous studies showed that the early use of calpain inhibitors reduces calpain activity in multiple brain regions, and that postnatal treatment with calpeptin may lead to cerebellar motor dysfunction. However, it remains unclear whether postnatal calpeptin application affects hippocampus-related behaviors. In this study, Sprague-Dawley rats were purchased from the Animal Center of Anhui Medical University of China. For the experiments in the adult stage, rats were intraperitoneally injected with calpeptin, 2 mg/kg, once a day, on postnatal days 7–14. Then on postnatal day 60, the Morris water maze test was used to evaluate spatial learning and memory abilities. The open field test was carried out to assess anxiety-like activities. Phalloidin staining was performed to observe synaptic morphology in the hippocampus. Immunohistochemistry was used to count the number of NeuN-positive cells in the hippocampal CA1 region. DiI was applied to label dendritic spines. Calpeptin administration impaired spatial memory, caused anxiety-like behavior in adulthood, reduced the number and area of apical dendritic spines, and decreased actin polymerization in the hippocampus, but did not affect the number of NeuN-positive cells in the hippocampal CA1 region. For the neonatal experiments, neonatal rats were intraperitoneally injected with calpeptin, 2 mg/kg, on postnatal days 7 and 8. Western blot assay was performed to analyze the protein levels of Akt, Erk, p-Akt, p-Erk1/2, Erk1/2, SCOP, PTEN, mTOR, p-mTOR, CREB and p-CREB in the hippocampus. SCOP expression was increased, and the phosphorylation levels of Akt, mTOR and CREB were reduced in the hippocampus. These findings show that calpeptin administration after birth affects synaptic development in neonatal rats by inhibiting the Akt/mTOR signaling pathway, thereby perturbing hippocampal function. Therefore, calpeptin administration after birth is a risk factor for neurodevelopmental defects.
文摘Aim： To immunolocalize the c-mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development. Methods： Semi-quantitative reverse transcription-polymerase chain reaction （RT- PCR） and in situ hybridization techniques were used to examine c-mos mRNA and indirect immunofluorescence was used to localize c-Mos protein in mouse testis on postnatal days 14, 21, 25, 28, 30, 35, 49 and 70. Results： c-mos mRNA remained low on postnatal days 14-21, increased abruptly from day 25 and peaked on day 30. Its levels decreased a little on day 35 and became almost stable thereafter until day 70. c-mos mRNA was localized in the nucleus and cytoplasm of the spermatocytes and round spermatids. The nuclear staining was much stronger than the cytoplasmic staining. Using a polyclonal anti-c-Mos antibody, Western blotting detected a single band at 43 kDa in testis lysate, c-Mos protein was exclusively localized to the elongating spermatids and was first detected on postnatal day 30. The number of c-Mos-positive spermatids increased progressively till day 49 and stabilized thereafter. Conclusion： The c-mos gene displays a spatial and temporal expression pattern in the mouse testis during postnatal development at both the mRNA and protein level. This suggests that c-mos might play important roles in spermatogenesis. （Asian J Androl 2008 Mar; 10： 277-285）
文摘The study aimed to determine whether antenatal fear of childbirth (expectation) could predict postnatal fear of childbirth (experience) by taking account of other antenatal psychological variables (anxiety and depression) as well as birth outcomes in Japanese women. A longitudinal observational study was conducted at a clinic in Tokyo, Japan, in 2011. Self-report questionnaires were distributed to 240 Japanese women at 37 gestational weeks (Time 1) and on the second day after delivery (Time 2). Regression analyses by means of structural equation modelling were conducted in both the primiparous and the multiparous group. The models exhibited good fit (chisquare value/degree of freedom = 1.10 - 1.62, comparative fit index = 0.92 - 0.99 and root mean square error of approximation = 0.03 - 0.07). Antenatal fear of childbirth was the most predictive variable of postnatal fear of childbirth in both the primiparous (β = 0.58, p = 0.002) and the multiparous group (β = 0.62, p < 0.001). In conclusion, antenatal fear of childbirth was a significant predictor of postnatal fear of childbirth when other antenatal psychological variables and birth outcomes were taken into account. Pregnant women who are strongly afraid of childbirth need special attention before and after delivery.
基金supported by the National Natural Science Foundation of China (31902133, 31972541, 31702088)Guangdong Provincial key Laboratory of Animal Molecular Design and Precise Breeding Research Grant(2019B030301010)+2 种基金the Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes (2019KSYS011)National Nonprofit Institute Research Grant (Y2016JC07 and 2018-YWF-YB-7)Foshan University Initiative Scientific Research Program。
文摘Background: RNA editing is a widespread post-transcriptional modification mechanism in mammalian genomes.Although many editing sites have been identified in domestic pigs(Sus scrofa), little is known about the characteristics and dynamic regulation of RNA editing in the pineal gland(PG), a small neuroendocrine gland that synthesizes and secretes melatonin, which is primarily responsible to modulate sleep patterns.Results: This study analyzed the expression of adenosine-to-inosine(A-to-I) editing regulators and profiled the first dynamic A-to-I RNA editome during postnatal PG development. The results identified ADAR1 as the most abundantly expressed ADAR enzyme, which was down-regulated during postnatal PG development. Furthermore,47,284 high-confidence RNA editing sites were identified, the majority of which(93.6%) were of the canonical A-to-I editing type, followed by C-to-T editing. Analysis of its characteristics showed that the A-to-I editing sites mostly localized in SINE retrotransposons PRE-1/Pre0_SS. Moreover, a strong deficiency and preference for guanine nucleotides at positions of one base upstream or downstream were found, respectively. The overall editing level at the puberty stage was higher than at both infancy and adulthood stages. Additionally, genome-wide RNA editing was found to exhibit a dynamic stage-specific fashion(postnatally). Genes that underwent developmental changes in RNA editing were associated with catabolic processes as well as protein localization and transport functions,implying that RNA editing might be responsible for the molecular machineries of the postnatal developing PG.Remarkably, RNA editing in 3′-UTRs might regulate gene expression by influencing miRNA binding during PG development.Conclusions: This study profiles the first comprehensive developmental RNA editome in the pig PG, which contributes to the understanding of the importance of post-transcriptionally mediated regulation during mammalian postnatal PG development. Moreover, this study widely extends RNA editome resources in mammals.
文摘To investigate the expression pattern of rat Eppin （epididymal protease inhibitor; official symbol Spinlwl）, we detected mRNA transcripts and subsequent protein translation of Eppin in several sorts of tissues by RT-PCR and westem blotting. Then immunohistochemistry was performed for more detailed observation. The testicular transcription level was monitored by real-time PCR throughout postnatal development. We found that rat Eppin was specifically expressed in the testis and epididymis. The testicular transcription was slight in neonatal （1-day） and infantile stages （5-, 7- and 10-day）. It increased sharply thereafter, with maximum expression level （about 38- fold compared with that of 1-day old rat） detected in prepubertal stage （15-day）. Then a slightly declined but stable level （about 20-fold compared with that of 1-day old rat） was kept in pubertal-early adult （30-day） and adult （60-day） stages of postnatal maturation. In the adult rat, EPPIN protein was mainly localized in the elongated spermatids and epididymal epithelial cells. Sperm in the epididymal duct were all covered with EPPIN and its level kept constant during incubation under conditions used to achieve capacitation. Its stage-specific expression in the testis suggests that EPPIN may be important during spermatogenesis especially for the spermatid elongation. The abundant production of epididymal EPPIN indicated indirectly that it might play a role in the function of the epididymis.
基金supported by a Johns Hopkins Musculoskeletal Research Pilot and Feasibility Grant (RKT) and NIH R01 AR062074 (DJD)
文摘A large body of literature suggests that bone metabolism is susceptible to the ill effects of reactive species that accumulate in the body and cause cellular dysfunction. One of the body＇s front lines in defense against such damage is the transcription factor, Nrf2. This transcription factor regulates a plethora of antioxidant and cellular defense pathways to protect cells from such damage. Despite the breadth of knowledge of both the function of Nrf2 and the effects of reactive species in bone metabolism, the direct role of Nrf2 in skeletal biology has yet to be thoroughly examined. Thus, in the current study, we have examined the role of Nrf2 in postnatal bone metabolism in mice. Mice lacking Nrf2 （Nrf2-/-） exhibited a marked deficit in postnatal bone acquisition, which was most severe at 3 weeks of age when osteoblast numbers were 12-fold less than observed in control animals. While primary osteoblasts from Nrf2-/- mice functioned normally in vitro, the colony forming capacity of bone marrow stromal cells （BMSCs） from these mice was significantly reduced compared to controls. This defect could be rescued through treatment with the radical scavenger N-acetyl cysteine （NAC）, suggesting that increased reactive species stress might impair early osteoblastogenesis in BMSCs and lead to the failure of bone acquisition observed in Nrf2-/- animals. Taken together, these studies suggest Nrf2 represents a key pathway in regulating bone metabolism, which may provide future therapeutic targets to treat osteoporosis.
基金the Fundamental Research Funds for the Central Universities,No.21609101the National Basic Research Program of China (973 Program),No.2011CB707501
文摘Nitric oxide is an important neuromodulator in the brain and is involved in the development of visual system. But it is not clear how nitric oxide and nitric oxide synthase （NOS） are involved in the developing visual cortex of rodents. Thus we examined the expression of NOS activity in the postnatal developing visual cortex of the golden hamster by using histochemical technique for NADPH-diaphorase （NADPH-d）. A heavily stained NADPH-d band was observed in the neuropil of the visual cortex. This NADPH-d band initially appeared in the cortical plate from the day of birth （P0） to postnatal day 4 （P4）. From P7 to P21, this band was confined to area 17 and migrated to the deeper layers Ill IV and V VI before it eventually disappeared at P28. Such developmental trends of the band correlated well with the process of formation and establishment of the geniculo-cortical projection patterns. Thus, the areal specific development of the band suggests that NOS is closely related to the cortical differentiation and synaptic formation of the primary visual cortex. On the other hand, monocular eye enucleation on P1 could not alter the appearance of this NADPH-d positive band, indicating a non-activity dependant role of NOS. In addition, differences in the laminar distributions and developmental sequence between the heavily and lightly stained NADPH-d positive neurons during development suggest that they play different roles in the development.
文摘Fetal eutaneous wounds that oeeur in earlygestation heal without sear formation.Althoughmueh work has been done to eharaeterize the roleof transforming growth
基金supported financially by a “crédits incitatifs” grant from the department of Animal Physiology and Livestock Systems(PHASE)at INRAa grant from the Integrated Management of Animal Health metaprogram of INRA for the “GISA-WHELP” project(www.gisa.inra.fr/en)
文摘Background: Negative experiences in early life can induce long-lasting effects on the welfare, health, and performance of farm animals. A delayed placement of chicks in rearing houses has negative effects on their performance, and results in fecal-specific odors detectable by rats. Based on this observation, the volatile organic compounds(VOCs) and metabolites from the feces of 12-day-old chickens were screened for early markers of response to negative events using gas-chromatography and liquid-chromatography coupled with mass spectrometry(GC-MS, LC-HRMS).Results: The low reproducibility of solid-phase micro-extraction of the VOCs followed by GC-MS was not suitable for marker discovery, in contrast to liquid extraction of metabolites from freeze-dried feces followed by GC-MS or LC-HRMS analysis. Therefore, the fecal metabolome from 12-day-old chicks having experienced a normal or delayed placement were recorded by GC-MS and LC-HRMS in two genotypes from two experiments. From both experiments, 25 and 35 metabolites, respectively explaining 81% and 45% of the difference between delayed and control chickens, were identified by orthogonal partial least-squares discriminant analysis from LC-HRMS and GC-MS profiling.Conclusion: The sets of molecules identified will be useful to better understand the chicks’ response to negative events over time and will contribute to define stress or welfare biomarkers.
文摘AIM: To suggest a novel retinopathy of prematurity(ROP) screening model in developing countries incorporating postnatal weight gain ratios(PWGR) to traditional parameters to maintain sensitivity and improve specificity in detecting ROP. METHODS: Analysis of weekly PWGR of infants from one tertiary referral center during six months to determine the age at which the PWGR with the highest predictability for ROP development which was referred to as the postnatal net weight gain ratio(NWGR). NWGR was added to conventional criteria to describe a new model(The Alex-ROP model). RESULTS: Of 560 infants were included. NWGR 28 d after birth was the most predictive factor for the development of ROP. A new model Alex-ROP recommending screening infants with gestational age(GA) ≤33 wk or birth weight ≤1500 g and NWGR at 28 d after birth <0.3 was suggested. A second screening model referred to as High-grade AlexROP(Hg Alex-ROP) model to detect worse grade ROP(Both type 1 and type 2) recommending a cutoff point of NWGR <0.15 between birth and 28^(th) day. CONCLUSION: Both Alex-ROP and Hg Alex-ROP models are easy to apply to improve the specificity of ROP screening in developing countries while maintaining high sensitivity.
文摘To define the function of Zbtb20 in vivo,we generated knockout mice by homologous recombination.Zbtb20 null mice displayed a stark phenotype characterized by postnatal growth retardation,
基金This work was supported by grants from the Spanish Ministerio de Ciencia y Tecnología(BFU2007-67540)Ministerio de Economía y Competitividad(CGL2015-64650P)+1 种基金Dirección General de Investigación del Ministerio de Educación y Ciencia(BFU2017-85547-P)Junta de Extremadura,Fondo Europeo de Desarrollo Regional,“Una manera de hacer Europa”(GR15158,GR18114,IB18113).
文摘The visual system is affected by neurodegenerative diseases caused by the degeneration of specific retinal neurons,the leading cause of irreversible blindness in humans.Throughout vertebrate phylogeny,the retina has two kinds of specialized niches of constitutive neurogenesis:the retinal progenitors located in the circumferential marginal zone and Müller glia.The proliferative activity in the retinal progenitors located in the circumferential marginal zone in precocial birds such as the chicken,the commonest bird model used in developmental and regenerative studies,is very low.This region adds only a few retinal cells to the peripheral edge of the retina during several months after hatching,but does not seem to be involved in retinal regeneration.Müller cells in the chicken retina are not proliferative under physiological conditions,but after acute damage some of them undergo a reprogramming event,dedifferentiating into retinal stem cells and generating new retinal neurons.Therefore,regenerative response after injury occurs with low efficiency in the precocial avian retina.In contrast,it has recently been shown that neurogenesis is intense in the retina of altricial birds at hatching.In particular,abundant proliferative activity is detected both in the circumferential marginal zone and in the outer half of the inner nuclear layer.Therefore,stem cell niches are very active in the retina of altricial birds.Although more extensive research is needed to assess the potential of proliferating cells in the adult retina of altricial birds,it emerges as an attractive model for studying different aspects of neurogenesis and neural regeneration in vertebrates.
基金This work was supported by the National Natural Science Foundation of China(31930047,91732304 and 31630031)The Strategic Priority Research Program of the Chinese Academy of Science(XDB32030100)+9 种基金Guangdong Provincial Key Laboratory of Brain Connectome and Behavior(2017B030301017)the Natural,Science Foundationo Gfuangdong,Province(2018A030313439)a Shenzhen Government grant(JCYJ20170413164535041)Shenzhen Key Science and Technology Infrastructure Planning Project(ZDKJ20190204002)Key Laboratory of CAS(2019DP173024)The Ten Thousand Talents ProgramThe Guangdong Special Support ProgramChang Jiang Scholars ProgramIntermational PartnershipProgram of theChinese Academy of Sciences(172644KYS820170004)and the Key-Area Research and Development Program of Guangdong Province(2018B030331001).
文摘Environmental threats often trigger innate defensive responses in mammals.However,the gradual development of functional properties of these responses during the postnatal development stage remains unclear.Here,we report that looming stimulation in mice evoked fight behavior commencing at P14-16 and had fully developed by P20-24.The visual-evoked innate defensive response was not significantly altered by sensory deprivation at an early postnatal stage.Furthermore,the percentages of wide-field and horizontal cells in the superior colliculus were notably elevated at P20-24.Our findings define a developmental time window for the formation of the visual innate defense response during the early postnatal period and_provide important insight into the underlying mechanism.
基金supported by Ege Universtity Hospitalfunded by the State Planning Organization,Turkey.
文摘Aim: This randomize controlled experimental study has been carried out to investigate the effects on the integrity of perine of perineal massage and Kegel exercises applied prenatally to women who experienced vaginal delivery. Methods: Research was carried out between January 2012 and 2013, with a total of 101 pregnant women who referred to Ege University Hospital in ?zmir. Data Collection Form, Kegel Exercise Training Brochure, Practice Observation Form and Prenatal Perinea Massage Learning Guide for Implementer were used. Researcher continued to perform this massage once a week until delivery. Kegel exercises were asked to perform exercises at home and also to register them until delivery. When exercise group came to weekly controls or when they were contacted at home they were asked if they have performed daily exercise or not. The pregnant women in control group did not receive any application. One to one interview was performed during delivery and postnatal 24 hours at the hospital and a telephone interview was performed 15 days postnatal, so three groups were evaluated. The chi-square, Mann-Whitney U test and Kruskal Wallis test were used. Results: A statistically significant difference was found between study and control groups in terms of episiotomy rates, laceration, postnatal 24 hours and 15 days perineal pain and improvement (p < 0.05). Conclusion: It was found that perineal massage and Kegel exercises are important in maintaining the integrity of perineal significantly. It is thought that when the perineal massage and Kegel exercise being performed during pregnancy is supported by health professional, it will play a significant role in women’s quality of life.
