An advanced approach for functionalizing the surfaces of electrospun poly(l-lactide-co-ε-caprolactone)(PLCL)nanofibers for biomedical applications is presented here.Using initiated chemical vapor deposition(iCVD),a c...An advanced approach for functionalizing the surfaces of electrospun poly(l-lactide-co-ε-caprolactone)(PLCL)nanofibers for biomedical applications is presented here.Using initiated chemical vapor deposition(iCVD),a coating of the copolymer p(PFMA-co-DVB)containing poly(pentafluorophenyl methacrylate)(PFMA)and divinylbenzene(DVB)was applied to the PLCL nanofibers.This coating facilitated efficient immobilization of the biomolecules on the PLCL nanofiber surfaces,allowing precise adjustments to the polymer composition through modulation of the monomer flow rates.The resulting copolymer exhibited superior efficiency for immobilizing IgG,as confirmed by immunofluorescence intensity analysis.In vitro studies conducted with different neural cell types demonstrated that the laminin-coated iCVD-functionalized PLCL nanofibers maintained their inherent biocompatibility while significantly enhancing cell adhesion.By exploiting the elastic nature of the PLCL nanofibers,cell elongation could be successfully manipulated by controlling the nanofiber alignment,as demonstrated by scanning electron microscopy and quantification of the immunofluorescence image orientation.These findings highlight the potential of iCVD-modified PLCL nanofibers as versatile platforms for neural tissue engineering and various biomedical applications,allowing valuable biomaterial surface modifications for enhanced cellular interactions.展开更多
基金supported by the National R&D Program through the National Research Foundation of Korea(NRF)grant funded by the Ministry of Science and ICT(Grant Nos.NRF-2021M3H4A4079294,RS-2023-00211412)the KIST research programs(2E32860,2E32911,2E33151).
文摘An advanced approach for functionalizing the surfaces of electrospun poly(l-lactide-co-ε-caprolactone)(PLCL)nanofibers for biomedical applications is presented here.Using initiated chemical vapor deposition(iCVD),a coating of the copolymer p(PFMA-co-DVB)containing poly(pentafluorophenyl methacrylate)(PFMA)and divinylbenzene(DVB)was applied to the PLCL nanofibers.This coating facilitated efficient immobilization of the biomolecules on the PLCL nanofiber surfaces,allowing precise adjustments to the polymer composition through modulation of the monomer flow rates.The resulting copolymer exhibited superior efficiency for immobilizing IgG,as confirmed by immunofluorescence intensity analysis.In vitro studies conducted with different neural cell types demonstrated that the laminin-coated iCVD-functionalized PLCL nanofibers maintained their inherent biocompatibility while significantly enhancing cell adhesion.By exploiting the elastic nature of the PLCL nanofibers,cell elongation could be successfully manipulated by controlling the nanofiber alignment,as demonstrated by scanning electron microscopy and quantification of the immunofluorescence image orientation.These findings highlight the potential of iCVD-modified PLCL nanofibers as versatile platforms for neural tissue engineering and various biomedical applications,allowing valuable biomaterial surface modifications for enhanced cellular interactions.
