Presently,Saccharomyces cerevisiae demonstrates proficient co-fermentation of glucose and xylose,marking a significant advancement in second-generation fuel ethanol production.However,the presence of high concen-trati...Presently,Saccharomyces cerevisiae demonstrates proficient co-fermentation of glucose and xylose,marking a significant advancement in second-generation fuel ethanol production.However,the presence of high concen-trations of inhibitors in industrial lignocellulose hydrolysates and post-glucose effect caused by glucose con-sumption hinders severely impedes yeast robustness and xylose utilization for ethanol fermentation.Even worse,the antagonism between xylose utilization ability and strain robustness was observed,which proposes a difficult challenge in the production of second-generation fuel ethanol by S.cerevisiae.This review introduces the effect of engineering transcriptional regulatory networks on enhancing xylose utilization,improving strain robustness,alleviating antagonism between xylose utilization and strain robustness,and reducing post-glucose effect.Additionally,we provide an outlook on the developmental trends in this field,offering insights into future di-rections for increasing the production of second-generation fuel ethanol in S.cerevisiae.展开更多
基金supported by the National Key Research and Development Project of China(2018YFB1501401)the Key innovation Project of Qilu University of Technology(Shandong Academy of Sciences)(2024ZDZX03)+1 种基金the Shandong Provincial Technical Innovation Boot Program(02055183)the National Natural Science Foundation of China(31870063).
文摘Presently,Saccharomyces cerevisiae demonstrates proficient co-fermentation of glucose and xylose,marking a significant advancement in second-generation fuel ethanol production.However,the presence of high concen-trations of inhibitors in industrial lignocellulose hydrolysates and post-glucose effect caused by glucose con-sumption hinders severely impedes yeast robustness and xylose utilization for ethanol fermentation.Even worse,the antagonism between xylose utilization ability and strain robustness was observed,which proposes a difficult challenge in the production of second-generation fuel ethanol by S.cerevisiae.This review introduces the effect of engineering transcriptional regulatory networks on enhancing xylose utilization,improving strain robustness,alleviating antagonism between xylose utilization and strain robustness,and reducing post-glucose effect.Additionally,we provide an outlook on the developmental trends in this field,offering insights into future di-rections for increasing the production of second-generation fuel ethanol in S.cerevisiae.
