Objective:To explore the pharmacodynamic and renal protective effects of Poriae cutis extract on hyperuricemia(HUA)mice.Methods:The active components from the Poriae cutis were extracted with Alcohol solvent and aqueo...Objective:To explore the pharmacodynamic and renal protective effects of Poriae cutis extract on hyperuricemia(HUA)mice.Methods:The active components from the Poriae cutis were extracted with Alcohol solvent and aqueous solvent,HUA mice were established by hypoxanthine with potassium oxonate.Totally70 male Kunming mice were randomly divided into normal,model(500 mg/kg hypoxanthine+250 mg/kg potassium oxonate),Poriae cutis ethanol(PCE)and water extract(PCW)with high and low dose(388 mg/kg and 97mg/kg),benzbromarone(BEN)groups(7.5 mg/kg),all mice were administered with corresponding medication for 16 d.After the last administration,collected the 24 h urine volume,the urine uric acid(UUA)and urine creatinine(UCr)were determined.The morphological changes of the left kidney were viewed and the pathological changes in right kidneys were observed by hematoxylin-eosin(HE)staining.The serum contents of uric acid(SUA),creatinine(SCr)and urea nitrogen(BUN)were measured.Uric acid excretion index include fractional uric acid excretion(FEUA)and uric acid clearance rate(CUr)were calculated.The contents of OAT1,URAT1,GLUT9,TNF-αand IL-6 in the kidney were detected by ELISA.Results:PCE and PCW could increase UUA,UCr,FEUA,CUr and OAT1(P<0.05,P<0.01),reduce SUA,SCr,BUN,URAT1,GLUT9,TNF-αand IL-6(P<0.05,P<0.01).The pathological and morphological changes of kidneys in HUA mice were improved and the effect is better than BEN.Conclusion:Poriae cutis extractscould lower uric acid and possessed a protective effect on the kidney of mice with HUA,which was achieved by promoting uric acid excretion,regulating the expression of uric acid transporters and reducing the level of inflammatory factors.展开更多
[Objectives]To study the immediate-release mechanism of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets by liquid-solid compacts technique.[Methods] Taking dehydrotramete...[Objectives]To study the immediate-release mechanism of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets by liquid-solid compacts technique.[Methods] Taking dehydrotrametenolic acid and dehydroeburicoic acid as indicators,differences in dissolution were compared between liquid-solid compressed tablets and crude drug of total triterpenoids in Poriae Cutis;crude drug,powder of liquid-solid compressed tablets and excipients of liquid-solid compressed tablets were characterized by differential scanning calorimetry( DSC).[Results]Liquid-solid compact technique could significantly improve dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid. The total dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets was 92%,t50( time for 50% total dissolution rate) and t_D( time for 63. 2% total dissolution rate) were 11. 18 min and 22. 71 min; the total dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid in crude drug of total triterpenoids was 29%,and t50 and t_D were231. 06 min and 359. 23 min. DSC showed that there was no mutual interaction between excipients and total triterpenoids in Poriae Cutis; on the DSC curve for powder of liquid-solid compressed tablets,the absorption peak vanished completely,indicating that the drug exists in the amorphous form in the liquid-solid powder.[Conclusions] Liquid-solid compact technique can increase the dissolution rate of total triterpenoids in Poriae Cutis and allow rapid release of poorly water soluble drugs.展开更多
Background Porcine epidemic diarrhea virus(PEDV)infection poses a significant challenge to the swine industry,with limited effective control measures available.Poria cocos polysaccharides(PCP)is the primary active ing...Background Porcine epidemic diarrhea virus(PEDV)infection poses a significant challenge to the swine industry,with limited effective control measures available.Poria cocos polysaccharides(PCP)is the primary active ingredient of Poria cocos,and has been demonstrated to show beneficial effects on intestinal damage in previous studies.However,its mechanism has not been fully understood.In the present study,18 seven-day-old piglets were divided into 3 groups:Control group,PEDV group,and PCP+PEDV group.After three days of adaptation,piglets in the PCP+PEDV group were orally administered 10 mg/kg body weight/d PCP from d 4 to 10.On d 8,piglets were orally administered with PEDV at the dose of 104.5 TCID50/piglet.This study aimed to investigate the potential effects of PCP on PEDVinduced intestinal injury and explored the underlying mechanisms.Results The results showed that PCP administration effectively alleviated diarrhea,reduced PEDV replication in the small intestine and colon of piglets,and significantly improved intestinal mucosal morphology.Specifically,PCP increased the villus height in both the jejunum and ileum and increased the villus height to crypt depth ratio in the ileum(P<0.05).Improved intestinal function was further evidenced by elevated plasma D-xylose levels and decreased diamine oxidase activity(P<0.05).Transcriptomic and proteomic analyses revealed that lipid metabolism is a key pathway regulated by PCP during PEDV infection.Notably,PCP significantly upregulated sphingolipid metabolism-related genes,including ectonucleotide pyrophosphatase/phosphodiesterase family member 7 and N-acylsphingosine amidohydrolase 2.Metabolomic analysis revealed that PCP primarily modulated the levels of plasmanylphosphoethanolamine,lysophosphatidylcholine,and carnitine.Additionally,PCP reversed the expression of key genes involved in fatty acid uptake,intracellular lipid transport,and fatty acid synthesis,such as fatty acid binding protein 2,fatty acid transport protein 4,apolipoprotein B,apolipoprotein C3,fatty acid synthase,long-chain fatty acyl CoA synthetase 3,lipoprotein lipase and acyl-CoA thioesterases 12(P<0.05).Conclusions These findings demonstrate that PCP mitigates PEDV-induced intestinal injury by modulating lipid metabolism and highlight its potential as a dietary supplement for enhancing anti-PEDV defenses and promoting intestinal health in piglets.展开更多
Objective:Microneedles(MNs),as a key component of third-generation transdermal drug delivery sys-tems,show strong potential for obesity treatment.This study aimed to integrate traditional Chinese medicine(TCM)therapie...Objective:Microneedles(MNs),as a key component of third-generation transdermal drug delivery sys-tems,show strong potential for obesity treatment.This study aimed to integrate traditional Chinese medicine(TCM)therapies,including Chinese patent medicine(CPM)injections and patches,with MN technology to identify commonly used administration sites and Chinese medicinal material(CMM)con-stituents suitable for MN-based weight loss interventions.Methods:Literature was retrieved from PubMed,Web of Science,Google Scholar,CNKI,and Google Patents.First,existing studies on MN-based weight loss were narratively reviewed.Then,studies on CPM injections or patches were analyzed to extract intervention elements,including administration sites,CMM constituents,and symptoms.Acupoint-symptom and CMM constituent-symptom pairs were compiled,and key nodes were identified through complex network analysis using eigenvector centrality,PageRank,and betweenness centrality.Results:Forty-four studies and thirteen patents were included.The review indicated that MN-based in-terventions demonstrated significant weight loss effects;however,current research remains limited by a focus on fat-deposition sites and insufficient development of CMM carriers.Network analysis identi-fied Guanyuan(CV4)and Poria cocos(Fuling)as central nodes across all metrics,suggesting their strong potential as key elements in MN-based therapies.Conclusion:CV4 and Poria cocos represent promising candidates for delivery sites and CMM constituents in MN-mediated obesity treatment.By integrating TCM principles with modern MN technology,these findings provide a theoretical basis for developing more targeted,efficient,and integrative anti-obesity interventions.展开更多
This study was conducted to extract pachyman to the greatest degree with ultrasonic technology, and to determine the content of pachyman by the phe-nol-sulfuric acid method, so as to choose the optimal technique of ul...This study was conducted to extract pachyman to the greatest degree with ultrasonic technology, and to determine the content of pachyman by the phe-nol-sulfuric acid method, so as to choose the optimal technique of ultrasonic extrac-tion of pachyman. Firstly, the ultrasonic power, extraction time and solid-to-liquid ra-tio were investigated by ultrasonic extraction method. Then, on this basis, a respon-sible surface experiment was designed, to select the optimal process conditions ac-cording to the index of pachyman yield. The optimized ultrasonic water extraction conditions were as fol ow： ultrasonic power of 100 W, extraction time of 30 min, and solid-to-liquid ratio at 1：40. The Oxford cup method was used to study the an-timicrobial activity of pachyman. lt was found that pachyman could significantly in-hibit Bacilus subtilis_and Staphylococcus aureus.展开更多
Based on extensive literature search, the composition, pharmacological ac- tion and clinical application of Poria with hostwood have been summarized, to pro- vide scientific data for further research ＆ development.
In this study, with Jiuzihe poria as the main raw material, the effect of alkali concentration, extraction time and solid-liquid ratio on the extraction rate of alkali-soluble polysaccharide was investigated, and the ...In this study, with Jiuzihe poria as the main raw material, the effect of alkali concentration, extraction time and solid-liquid ratio on the extraction rate of alkali-soluble polysaccharide was investigated, and the processing technology was optimized with orthogonal experiment. The results showed that the optimum extraction process for alkali-soluble polysacchadde from poria was as follows： solid-liquid ratio of 1：50, alkali concentration of 0.5 mol/L, and extraction time of 8 h.展开更多
基金Anhui University Collaborative Project(GXXT-2019-043)Key Project of Anhui University Scientific Research Project(KJ2020A0608)。
文摘Objective:To explore the pharmacodynamic and renal protective effects of Poriae cutis extract on hyperuricemia(HUA)mice.Methods:The active components from the Poriae cutis were extracted with Alcohol solvent and aqueous solvent,HUA mice were established by hypoxanthine with potassium oxonate.Totally70 male Kunming mice were randomly divided into normal,model(500 mg/kg hypoxanthine+250 mg/kg potassium oxonate),Poriae cutis ethanol(PCE)and water extract(PCW)with high and low dose(388 mg/kg and 97mg/kg),benzbromarone(BEN)groups(7.5 mg/kg),all mice were administered with corresponding medication for 16 d.After the last administration,collected the 24 h urine volume,the urine uric acid(UUA)and urine creatinine(UCr)were determined.The morphological changes of the left kidney were viewed and the pathological changes in right kidneys were observed by hematoxylin-eosin(HE)staining.The serum contents of uric acid(SUA),creatinine(SCr)and urea nitrogen(BUN)were measured.Uric acid excretion index include fractional uric acid excretion(FEUA)and uric acid clearance rate(CUr)were calculated.The contents of OAT1,URAT1,GLUT9,TNF-αand IL-6 in the kidney were detected by ELISA.Results:PCE and PCW could increase UUA,UCr,FEUA,CUr and OAT1(P<0.05,P<0.01),reduce SUA,SCr,BUN,URAT1,GLUT9,TNF-αand IL-6(P<0.05,P<0.01).The pathological and morphological changes of kidneys in HUA mice were improved and the effect is better than BEN.Conclusion:Poriae cutis extractscould lower uric acid and possessed a protective effect on the kidney of mice with HUA,which was achieved by promoting uric acid excretion,regulating the expression of uric acid transporters and reducing the level of inflammatory factors.
基金Supported by Project of National Natural Science Foundation of Shandong Province(ZR2015PH015)
文摘[Objectives]To study the immediate-release mechanism of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets by liquid-solid compacts technique.[Methods] Taking dehydrotrametenolic acid and dehydroeburicoic acid as indicators,differences in dissolution were compared between liquid-solid compressed tablets and crude drug of total triterpenoids in Poriae Cutis;crude drug,powder of liquid-solid compressed tablets and excipients of liquid-solid compressed tablets were characterized by differential scanning calorimetry( DSC).[Results]Liquid-solid compact technique could significantly improve dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid. The total dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets was 92%,t50( time for 50% total dissolution rate) and t_D( time for 63. 2% total dissolution rate) were 11. 18 min and 22. 71 min; the total dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid in crude drug of total triterpenoids was 29%,and t50 and t_D were231. 06 min and 359. 23 min. DSC showed that there was no mutual interaction between excipients and total triterpenoids in Poriae Cutis; on the DSC curve for powder of liquid-solid compressed tablets,the absorption peak vanished completely,indicating that the drug exists in the amorphous form in the liquid-solid powder.[Conclusions] Liquid-solid compact technique can increase the dissolution rate of total triterpenoids in Poriae Cutis and allow rapid release of poorly water soluble drugs.
基金supported by the National Natural Science Foundation of China(32172763,U22A20514)the National Key R&D Program of China(2022YFD130040302)+1 种基金the Hubei Provincial Key R&D Program(2023BBB040)the Hubei Important Science and Technology Project(2024BBA004).
文摘Background Porcine epidemic diarrhea virus(PEDV)infection poses a significant challenge to the swine industry,with limited effective control measures available.Poria cocos polysaccharides(PCP)is the primary active ingredient of Poria cocos,and has been demonstrated to show beneficial effects on intestinal damage in previous studies.However,its mechanism has not been fully understood.In the present study,18 seven-day-old piglets were divided into 3 groups:Control group,PEDV group,and PCP+PEDV group.After three days of adaptation,piglets in the PCP+PEDV group were orally administered 10 mg/kg body weight/d PCP from d 4 to 10.On d 8,piglets were orally administered with PEDV at the dose of 104.5 TCID50/piglet.This study aimed to investigate the potential effects of PCP on PEDVinduced intestinal injury and explored the underlying mechanisms.Results The results showed that PCP administration effectively alleviated diarrhea,reduced PEDV replication in the small intestine and colon of piglets,and significantly improved intestinal mucosal morphology.Specifically,PCP increased the villus height in both the jejunum and ileum and increased the villus height to crypt depth ratio in the ileum(P<0.05).Improved intestinal function was further evidenced by elevated plasma D-xylose levels and decreased diamine oxidase activity(P<0.05).Transcriptomic and proteomic analyses revealed that lipid metabolism is a key pathway regulated by PCP during PEDV infection.Notably,PCP significantly upregulated sphingolipid metabolism-related genes,including ectonucleotide pyrophosphatase/phosphodiesterase family member 7 and N-acylsphingosine amidohydrolase 2.Metabolomic analysis revealed that PCP primarily modulated the levels of plasmanylphosphoethanolamine,lysophosphatidylcholine,and carnitine.Additionally,PCP reversed the expression of key genes involved in fatty acid uptake,intracellular lipid transport,and fatty acid synthesis,such as fatty acid binding protein 2,fatty acid transport protein 4,apolipoprotein B,apolipoprotein C3,fatty acid synthase,long-chain fatty acyl CoA synthetase 3,lipoprotein lipase and acyl-CoA thioesterases 12(P<0.05).Conclusions These findings demonstrate that PCP mitigates PEDV-induced intestinal injury by modulating lipid metabolism and highlight its potential as a dietary supplement for enhancing anti-PEDV defenses and promoting intestinal health in piglets.
文摘Objective:Microneedles(MNs),as a key component of third-generation transdermal drug delivery sys-tems,show strong potential for obesity treatment.This study aimed to integrate traditional Chinese medicine(TCM)therapies,including Chinese patent medicine(CPM)injections and patches,with MN technology to identify commonly used administration sites and Chinese medicinal material(CMM)con-stituents suitable for MN-based weight loss interventions.Methods:Literature was retrieved from PubMed,Web of Science,Google Scholar,CNKI,and Google Patents.First,existing studies on MN-based weight loss were narratively reviewed.Then,studies on CPM injections or patches were analyzed to extract intervention elements,including administration sites,CMM constituents,and symptoms.Acupoint-symptom and CMM constituent-symptom pairs were compiled,and key nodes were identified through complex network analysis using eigenvector centrality,PageRank,and betweenness centrality.Results:Forty-four studies and thirteen patents were included.The review indicated that MN-based in-terventions demonstrated significant weight loss effects;however,current research remains limited by a focus on fat-deposition sites and insufficient development of CMM carriers.Network analysis identi-fied Guanyuan(CV4)and Poria cocos(Fuling)as central nodes across all metrics,suggesting their strong potential as key elements in MN-based therapies.Conclusion:CV4 and Poria cocos represent promising candidates for delivery sites and CMM constituents in MN-mediated obesity treatment.By integrating TCM principles with modern MN technology,these findings provide a theoretical basis for developing more targeted,efficient,and integrative anti-obesity interventions.
基金Supported by Natural Science Foundation of Hubei Province(2013CFB472)~~
文摘This study was conducted to extract pachyman to the greatest degree with ultrasonic technology, and to determine the content of pachyman by the phe-nol-sulfuric acid method, so as to choose the optimal technique of ultrasonic extrac-tion of pachyman. Firstly, the ultrasonic power, extraction time and solid-to-liquid ra-tio were investigated by ultrasonic extraction method. Then, on this basis, a respon-sible surface experiment was designed, to select the optimal process conditions ac-cording to the index of pachyman yield. The optimized ultrasonic water extraction conditions were as fol ow： ultrasonic power of 100 W, extraction time of 30 min, and solid-to-liquid ratio at 1：40. The Oxford cup method was used to study the an-timicrobial activity of pachyman. lt was found that pachyman could significantly in-hibit Bacilus subtilis_and Staphylococcus aureus.
基金Supported by Open Fund of Ministry of Education’s Key Laboratory for Western Resource Biology and Biotechnology Laboratory(KH09030)Major Science and Technology Program of Science and Technology Office of the Tibet Autonomous Region(20091012)Scientific Research Plan Project of Shaanxi Education Department(2010JK862)~~
文摘Based on extensive literature search, the composition, pharmacological ac- tion and clinical application of Poria with hostwood have been summarized, to pro- vide scientific data for further research ＆ development.
文摘In this study, with Jiuzihe poria as the main raw material, the effect of alkali concentration, extraction time and solid-liquid ratio on the extraction rate of alkali-soluble polysaccharide was investigated, and the processing technology was optimized with orthogonal experiment. The results showed that the optimum extraction process for alkali-soluble polysacchadde from poria was as follows： solid-liquid ratio of 1：50, alkali concentration of 0.5 mol/L, and extraction time of 8 h.
