PBQ[1-(4-chlorophenyl)-3-(pyridin-3-yl)urea],an enormous potent molluscicide,showed excellent Pomacea canaliculata(P.canaliculata)control activity and low toxicity for other aquatic organisms,but its snail-killing mec...PBQ[1-(4-chlorophenyl)-3-(pyridin-3-yl)urea],an enormous potent molluscicide,showed excellent Pomacea canaliculata(P.canaliculata)control activity and low toxicity for other aquatic organisms,but its snail-killing mechanisms are still not fully understood.We employed an optical method to elucidate PBQ action via a novel fluorescent viscosity probe,NCV.As the viscosity in the test solutions increased,compared with that in pure ethanol,a 54-fold fluorescence intensity enhancement of NCV was observed in 310 cP of 90%glycerol.Furthermore,NCV successfully exhibited a selective fluorescence response towards monensin-induced cellular viscosity changes in HepG2 cells.The liver,stomach,and foot plantar of the tested snails were frozen and sectioned for fluorescent imaging experiments after the treatment with different PBQ concentrations over various times.A significant fluorescent increase in the snail's liver was observed upon exposure to 0.75 mg/L PBQ for 72 h,which highlighted an increase in viscosity.Hematoxylin and eosin(HE)staining further supported PBQ-induced liver damage with a viscosity increase in P.canaliculata.Our study provides a new rapid optical visualization method to study the killing mechanisms of PBQ and may help discover new chemicals that control snail populations.展开更多
The golden apple snail Pomacea canaliculata （Lamarck 1819） was first recorded at Black Dragon Spring, Dianchi Basin, Baiyi Township, Songming County, Kunming City, Yunnan Province, China, in October 2004. The water ...The golden apple snail Pomacea canaliculata （Lamarck 1819） was first recorded at Black Dragon Spring, Dianchi Basin, Baiyi Township, Songming County, Kunming City, Yunnan Province, China, in October 2004. The water from the spring flows into the Songhuaba Reservoir, the major drinking water resource for Kunming City, and part of the Dianchi Lake basin. This is the first record of this invasive snail in the Dianchi Lake Basin. Pomacea canaliculata originates from Central and South America, and in Asia the snail has spread through deliberate and accidental introductions to the Philippines, Vietnam, Thailand, Laos, Cambodia, Malaysia, Indonesia, Papua New Guinea, Korea, Japan and South China. It has become a major pest in rice-growing areas, resulting in huge damage to crops. Strict prevention and control measures have to be implemented to prevent the spread of the snail in Yunnan, together with public awareness campaigns to inform the public of the dangers of this invasive snail.展开更多
为了解和对比近些年来国内外福寿螺领域研究趋势和热点内容,本文以中国知网(CNKI)和Web of Science(WOS)核心文集TM的科学引文索引扩展版SCI-E数据库为数据来源,运用CiteSpace和VOSviewer软件对2009—2021年福寿螺属中入侵能力最强、入...为了解和对比近些年来国内外福寿螺领域研究趋势和热点内容,本文以中国知网(CNKI)和Web of Science(WOS)核心文集TM的科学引文索引扩展版SCI-E数据库为数据来源,运用CiteSpace和VOSviewer软件对2009—2021年福寿螺属中入侵能力最强、入侵范围最广的两种物种(Pomacea canaliculata和Pomacea maculate)研究领域的813篇相关文献(中文文献398篇,英文文献415篇)进行年度发文量、作者和国家合作网络、关键词共现、聚类和突现以及文献共被引分析.结果显示:自2009年来,国际上福寿螺领域研究热度持续,发文总体呈波浪式增长;国内研究热度有所减缓,发文总体呈下降趋势.在该领域内,国内发文地区主要集中在我国长江中下游以南及沿海地区;国际上,中国、阿根廷、美国、巴西是主要的发文国家.我国发文量位居第一,与阿根廷、美国构成该领域研究的主要国家力量.国家间合作密切,合作网络覆盖亚洲、南美、北美、西欧和澳大利亚各大区域.国内外近年来在该领域的研究热点有所不同,广州管圆线虫病、福寿螺的抗逆性和防治是我国近些年甚至未来很长一段时间研究关注的重点;在世界范围内,近年来的研究热点则是个体识别与鉴定、系统发育分析、物种地理起源和进化方面.此外,蛋白质组、卵、渐渗杂交福寿螺的危害和可利用化是目前国际在该领域的研究前沿.展开更多
The snails(Pomacea insularum) were collected from polluted and unpolluted sites at Juru River and the Universiti Putra Malaysia Lake,respectively.Besides the shells,the soft tissues of snails were dissected into 6 dif...The snails(Pomacea insularum) were collected from polluted and unpolluted sites at Juru River and the Universiti Putra Malaysia Lake,respectively.Besides the shells,the soft tissues of snails were dissected into 6 different parts: intestine,operculum,foot,pennial sac,lung sac and the remainder.They were analyzed for the concentrations of Cu,Cd,Zn,Pb,Ni and Fe.The present study found 6 interesting points.First,all the metal levels found in the different parts of P.insularum collected from Juru River were significantly(p<0.05) higher than those in the snails of unpolluted lake.These results were also supported by the sediment data in which the Juru River sediment had significantly(p<0.05) higher levels of metal than those in the unpolluted lake.Second,the high levels of Cd and Pb were found in the shell of P.insularum,indicating that it is a potential biomonitoring material for these metals.Third,intestine accumulated elevated levels of Zn,Ni and Fe and thus is a potential biomonitoring organ for the 3 metals.Fourth,high levels of Cu were found in the lung sac and this phenomenon could be related to this metal binding to metallothionein in this organ.Fifth,elevated level of Pb found in the soft tissues of P.insularum indicated that this snail is not suitable for human consumption since its pollution level was higher than the food safety guideline for Pb.Sixth,there was no significant difference(p>0.05) between the different sizes(small sizes: 30.5-33.2 mm;large sizes: 37.7-40.4 mm) of P.insularum.The first 5 points supported the use of different soft tissues of P.insularum as biomonitoring organs of heavy metal pollution in the freshwater ecosystem in Malaysia although further validation is required.展开更多
Poolingfield specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction(PCR)-based assay.We investigated a pooling strategy in...Poolingfield specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction(PCR)-based assay.We investigated a pooling strategy in diagnosis of Angiostrongylus canto-nensis in Pomacea canaliculata.Two settings of speci-mens were prepared,divided into portions and detected by multiplex PCR.Specimens A was 0.4490 g positive lung tissue of 28 larval nodes from four snails mixed with 1.310 g negative lung tissue from six snails and divided into 32 portions.Specimens B was 0.5448 g positive lung tissue with 26 larval nodes from two snails mixed with 1.092 g negative lung tissue from seven snails and divided into 48 portions.Repeated sampling was performed and sample size-accumulated positive rate curves were drawn.According to the sample size-accumulated positive rate curves,the appropriate sample size of the two specimens were 18 and 15,respectively,which is 0.36–0.58 to the total sample size.These test characteristics and the relevant factors to the sample size would need to be determined in much larger studies and more appropriately infield populations.The result indicates that the number of larval node is not the most important,nor the only factor to the sample size.It also implies the feasibility to detect A.cantonensis in P.canaliculata by pooling strategies.展开更多
基金the financial support of this work by the National Natural Science Foundation of China(Nos.82072309,22067019 and 22367023)the Major Science and Technology Projects in Yunnan Province(No.202402AE090006)+3 种基金the Project of Yunnan Characteristic Plant Screening and R&D Service CXO Platform(No.2022YKZY001)Yunnan Provincial Science and Technology Department Yunnan University Joint Special Project(No.202201BF070001-001)the Postgraduate Research Innovation Foundation of Yunnan University(No.KC-23234403)the Scientific Research Foundation Project of Yunnan Provincial Department of Education(No.2023Y0240)。
文摘PBQ[1-(4-chlorophenyl)-3-(pyridin-3-yl)urea],an enormous potent molluscicide,showed excellent Pomacea canaliculata(P.canaliculata)control activity and low toxicity for other aquatic organisms,but its snail-killing mechanisms are still not fully understood.We employed an optical method to elucidate PBQ action via a novel fluorescent viscosity probe,NCV.As the viscosity in the test solutions increased,compared with that in pure ethanol,a 54-fold fluorescence intensity enhancement of NCV was observed in 310 cP of 90%glycerol.Furthermore,NCV successfully exhibited a selective fluorescence response towards monensin-induced cellular viscosity changes in HepG2 cells.The liver,stomach,and foot plantar of the tested snails were frozen and sectioned for fluorescent imaging experiments after the treatment with different PBQ concentrations over various times.A significant fluorescent increase in the snail's liver was observed upon exposure to 0.75 mg/L PBQ for 72 h,which highlighted an increase in viscosity.Hematoxylin and eosin(HE)staining further supported PBQ-induced liver damage with a viscosity increase in P.canaliculata.Our study provides a new rapid optical visualization method to study the killing mechanisms of PBQ and may help discover new chemicals that control snail populations.
文摘The golden apple snail Pomacea canaliculata （Lamarck 1819） was first recorded at Black Dragon Spring, Dianchi Basin, Baiyi Township, Songming County, Kunming City, Yunnan Province, China, in October 2004. The water from the spring flows into the Songhuaba Reservoir, the major drinking water resource for Kunming City, and part of the Dianchi Lake basin. This is the first record of this invasive snail in the Dianchi Lake Basin. Pomacea canaliculata originates from Central and South America, and in Asia the snail has spread through deliberate and accidental introductions to the Philippines, Vietnam, Thailand, Laos, Cambodia, Malaysia, Indonesia, Papua New Guinea, Korea, Japan and South China. It has become a major pest in rice-growing areas, resulting in huge damage to crops. Strict prevention and control measures have to be implemented to prevent the spread of the snail in Yunnan, together with public awareness campaigns to inform the public of the dangers of this invasive snail.
文摘为了解和对比近些年来国内外福寿螺领域研究趋势和热点内容,本文以中国知网(CNKI)和Web of Science(WOS)核心文集TM的科学引文索引扩展版SCI-E数据库为数据来源,运用CiteSpace和VOSviewer软件对2009—2021年福寿螺属中入侵能力最强、入侵范围最广的两种物种(Pomacea canaliculata和Pomacea maculate)研究领域的813篇相关文献(中文文献398篇,英文文献415篇)进行年度发文量、作者和国家合作网络、关键词共现、聚类和突现以及文献共被引分析.结果显示:自2009年来,国际上福寿螺领域研究热度持续,发文总体呈波浪式增长;国内研究热度有所减缓,发文总体呈下降趋势.在该领域内,国内发文地区主要集中在我国长江中下游以南及沿海地区;国际上,中国、阿根廷、美国、巴西是主要的发文国家.我国发文量位居第一,与阿根廷、美国构成该领域研究的主要国家力量.国家间合作密切,合作网络覆盖亚洲、南美、北美、西欧和澳大利亚各大区域.国内外近年来在该领域的研究热点有所不同,广州管圆线虫病、福寿螺的抗逆性和防治是我国近些年甚至未来很长一段时间研究关注的重点;在世界范围内,近年来的研究热点则是个体识别与鉴定、系统发育分析、物种地理起源和进化方面.此外,蛋白质组、卵、渐渗杂交福寿螺的危害和可利用化是目前国际在该领域的研究前沿.
基金the financial support provided through the Research University Grant Scheme(RUGS)(Vote No.:91229)Universiti Putra Malaysia and e-Science Fund(VoteNo.:5450338)the Ministry of Science,Technology and Innovation,Malaysia
文摘The snails(Pomacea insularum) were collected from polluted and unpolluted sites at Juru River and the Universiti Putra Malaysia Lake,respectively.Besides the shells,the soft tissues of snails were dissected into 6 different parts: intestine,operculum,foot,pennial sac,lung sac and the remainder.They were analyzed for the concentrations of Cu,Cd,Zn,Pb,Ni and Fe.The present study found 6 interesting points.First,all the metal levels found in the different parts of P.insularum collected from Juru River were significantly(p<0.05) higher than those in the snails of unpolluted lake.These results were also supported by the sediment data in which the Juru River sediment had significantly(p<0.05) higher levels of metal than those in the unpolluted lake.Second,the high levels of Cd and Pb were found in the shell of P.insularum,indicating that it is a potential biomonitoring material for these metals.Third,intestine accumulated elevated levels of Zn,Ni and Fe and thus is a potential biomonitoring organ for the 3 metals.Fourth,high levels of Cu were found in the lung sac and this phenomenon could be related to this metal binding to metallothionein in this organ.Fifth,elevated level of Pb found in the soft tissues of P.insularum indicated that this snail is not suitable for human consumption since its pollution level was higher than the food safety guideline for Pb.Sixth,there was no significant difference(p>0.05) between the different sizes(small sizes: 30.5-33.2 mm;large sizes: 37.7-40.4 mm) of P.insularum.The first 5 points supported the use of different soft tissues of P.insularum as biomonitoring organs of heavy metal pollution in the freshwater ecosystem in Malaysia although further validation is required.
文摘Poolingfield specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction(PCR)-based assay.We investigated a pooling strategy in diagnosis of Angiostrongylus canto-nensis in Pomacea canaliculata.Two settings of speci-mens were prepared,divided into portions and detected by multiplex PCR.Specimens A was 0.4490 g positive lung tissue of 28 larval nodes from four snails mixed with 1.310 g negative lung tissue from six snails and divided into 32 portions.Specimens B was 0.5448 g positive lung tissue with 26 larval nodes from two snails mixed with 1.092 g negative lung tissue from seven snails and divided into 48 portions.Repeated sampling was performed and sample size-accumulated positive rate curves were drawn.According to the sample size-accumulated positive rate curves,the appropriate sample size of the two specimens were 18 and 15,respectively,which is 0.36–0.58 to the total sample size.These test characteristics and the relevant factors to the sample size would need to be determined in much larger studies and more appropriately infield populations.The result indicates that the number of larval node is not the most important,nor the only factor to the sample size.It also implies the feasibility to detect A.cantonensis in P.canaliculata by pooling strategies.
