[Objective] This study aimed to explore optimum conditions for papain-as- sisted extraction of polysaccharides from Agrocybe aegirit. [Method] Because enzyme is capable of destroying organizational structure of cells,...[Objective] This study aimed to explore optimum conditions for papain-as- sisted extraction of polysaccharides from Agrocybe aegirit. [Method] Because enzyme is capable of destroying organizational structure of cells, papain was selected to cat- alyze cells to let polysaccharides out. An orthogonal experiment was carried out to investigate the effects of hydrolysis temperature, hydrolysis time, enzyme concentra- tion and solid to liquid ratio on extraction of polysaccharides from A. aegirit. [Result] Through the orthogonal experiment, the optimum papain-assisted extraction conditions were obtained： hydrolysis temperature, 45 ℃; hydrolysis time, 2 h; enzyme concen- tration 2%; solid to liquid ratio, 1：80. [Conclusion] This study will provide a reference for extraction of polysaccharides from A. aegirit.展开更多
[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid rati...[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin. [Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests. Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively. [Results] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution (pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1:30. In addition,pumpkin polysaccharides had a strong activity of eliminating ·OH,but very weak activity to scavenge O-2. [Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.展开更多
Fumonisin B1(FB1)is a mycotoxin that is harmful to the nervous system;however,there is currently a lack of studies on plant compounds that alleviate the neurotoxicity of FB1.Polygonatum sibiricum polysaccharides exhib...Fumonisin B1(FB1)is a mycotoxin that is harmful to the nervous system;however,there is currently a lack of studies on plant compounds that alleviate the neurotoxicity of FB1.Polygonatum sibiricum polysaccharides exhibit neuroprotective effects,but it is unclear whether they protect against FB1-induced neurotoxicity.Here,Caenorhabditis elegans(C.elegans)was used to study the alleviation effects of P.sibiricum polysaccharide extract(PSPE)preprotection on FB1-induced neurotoxicity.PSPE pretreatment improved the behavioral deficits and neuronal damage in FB1-induced C.elegans.In addition,20-200μg/mL PSPE alleviated the oxidative damage in FB1-induced C.elegans by regulating the levels of reactive oxygen species,antioxidant enzyme activities and gene expression.Further studies have shown that PSPE pretreatment induced mitochondrial expression,increased ATP levels and mitochondrial membrane potential,and regulated mitochondrial oxidative phosphorylation and dynamics.Together,the protective effects of PSPE against FB1-induced neurotoxicity might be closely related with the reduction of oxidative stress and the regulation of mitochondria.展开更多
[Objective]This experiment aimed to study the extraction technology of Auricularia auricula polysaccharide（APS）.[Method] Polysaccharide yield was regarded as studying target.Based on the single factor experiment res...[Objective]This experiment aimed to study the extraction technology of Auricularia auricula polysaccharide（APS）.[Method] Polysaccharide yield was regarded as studying target.Based on the single factor experiment results,orthogonal experiment design was applied to study the effect of liquid-solid ratio,extraction time,extraction temperature on polysaccharide yield.[Result]The results showed that extraction temperature was an important factor that affects the yield of crude polysaccharide,the optimal technology condition of water extraction way was liquid-solid ratio of 1：70（g：ml）,4-hour extraction time with temperature at 100 ℃.[Conclusion] The yield of Auricularia auricular polysaccharide was 42.56% under the above mentioned condition.展开更多
Morchella septimelata polysaccharide(MSP)possesses multiple biological activities.In this study,double enzyme method was used to extract polysaccharides from Morchella septimelata.The basis of single factor experiment...Morchella septimelata polysaccharide(MSP)possesses multiple biological activities.In this study,double enzyme method was used to extract polysaccharides from Morchella septimelata.The basis of single factor experiment and response surface design was used to optimize the extraction process of polysaccharides.The optimal extraction conditions of polysaccharide were as follows:extraction time 2 h,extraction temperature 40.5℃,solid-liquid ratio 1:15(w/v),and the amount of double enzyme(cellulase:β-glucanase,mass ratio 1:2)2.0%.Under these conditions,the extraction yield of polysaccharide reached(14.86±0.12)%.展开更多
This study aimed to evaluate the feasibility of extracting polysaccharides with significant in vitro anti-aging activity from turmeric by-products.Turmeric polysaccharides were produced through hydrothermal extraction...This study aimed to evaluate the feasibility of extracting polysaccharides with significant in vitro anti-aging activity from turmeric by-products.Turmeric polysaccharides were produced through hydrothermal extraction,ethanol precipitation,and purification,with a total sugar content of 88.50%(w/w)and a molecular weight of 5.409×105 Da.Structural analysis revealed that the polysaccharide chain was primarily composed of→4)-α-D-glucopyranose-(1→,→2)-α-L-rhamnopyranose-(1→,→6)-α-D-glucopyranose-(1→,T-α-L-arabinofuranose-(1→,T-α-D-glucopyranose-(1→,and T-β-D-glucopyranose-(1→.In the H2O2-induced senescence model,turmeric polysaccharides greatly inhibited cell apoptosis,reduced SA-β-galactosidase activity,and downregulated the expression of cyclin-dependent kinase inhibitor 2A(p16)and tumor protein p53(p53)in the p53/cyclindependent kinase inhibitor 1A(p21)/p16 signaling pathway,with the dose of 0.05 mg/mL showing the most pronounced effects.Taken together,turmeric polysaccharides can serve as a promising anti-aging agent in functional foods and pharmaceuticals,and this study will contribute to the value-added utilization of turmeric by-products.展开更多
Cordyceps militaris,a medicinal and edible fungus,has garnered significant interest for its polysaccharides,known for their diverse physiological activities and health benefits.The purpose of this study was to extract...Cordyceps militaris,a medicinal and edible fungus,has garnered significant interest for its polysaccharides,known for their diverse physiological activities and health benefits.The purpose of this study was to extract polysaccharides(CM-1)from Cordyceps militaris fruiting bodies using hot water extraction and column chromatography,and evaluate its structural characteristics,antioxidant capacity,and in vivo absorption effects.Additionally,the study aimed to assess its protective effects against carbon tetrachloride(CCl4)induced liver injury in mice.The results showed that CM-1 significantly decreased the expression levels of serum ALT,AST,TNF-α,IL-6,and IL-1β.Pre-treatment with CM-1 also increased the activities of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px).Furthermore,histopathological examination of liver tissues confirmed the protective effects of CM-1 against acute liver injury induced by CCl4.These results indicate CM-1 promising potential as a dietary supplement for liver injury prevention and treatment.展开更多
Dictyophora rubrovolvata has beautiful shape,good taste and high nutritional value,and has anti-oxidation,anti-tumor,hypoglycemic and other effects.In this review,the biological characteristics,nutritive chemical comp...Dictyophora rubrovolvata has beautiful shape,good taste and high nutritional value,and has anti-oxidation,anti-tumor,hypoglycemic and other effects.In this review,the biological characteristics,nutritive chemical composition,polysaccharide extraction and functions,cultivation and pest control of D.rubrovolvata were intensively discussed and summarized,and the future research direction was also prospected.This study aims to provide future recommendations for the research of D.rubrovolvata and then promote the development of D.rubrovolvata industry.展开更多
基金Supported by Youth Fund of National Natural Science Foundation of China(31201242/C130405)Science Research Program of Education Department of Hubei Province(Q20143004)~~
文摘[Objective] This study aimed to explore optimum conditions for papain-as- sisted extraction of polysaccharides from Agrocybe aegirit. [Method] Because enzyme is capable of destroying organizational structure of cells, papain was selected to cat- alyze cells to let polysaccharides out. An orthogonal experiment was carried out to investigate the effects of hydrolysis temperature, hydrolysis time, enzyme concentra- tion and solid to liquid ratio on extraction of polysaccharides from A. aegirit. [Result] Through the orthogonal experiment, the optimum papain-assisted extraction conditions were obtained： hydrolysis temperature, 45 ℃; hydrolysis time, 2 h; enzyme concen- tration 2%; solid to liquid ratio, 1：80. [Conclusion] This study will provide a reference for extraction of polysaccharides from A. aegirit.
基金Supported by the Key Scientific and Technological Project of Henan Province (102102110157)the Scientific Research Found Project of Henan University of Urban Construction (2010JZD008)~~
文摘[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin. [Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests. Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively. [Results] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution (pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1:30. In addition,pumpkin polysaccharides had a strong activity of eliminating ·OH,but very weak activity to scavenge O-2. [Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.
基金supported by the National Natural Science Foundation of China(32125031)the fellowship of China Postdoctoral Science Foundation(2021TQ0128)+3 种基金National first-class discipline program of Food Science and Technology(JUFSTR20180303)Collaborative Innovation Center for Food Safety and Quality Control,Science and technology project of Chongzuo City(FA2020014)Research of Polygonatum sibiricum on Efficacy Determination and Intestinal Metabolism(190790)Some strains were provided by the CGC,which is funded by NIH Office of Research Infrastructure Programs(P40 OD010440).
文摘Fumonisin B1(FB1)is a mycotoxin that is harmful to the nervous system;however,there is currently a lack of studies on plant compounds that alleviate the neurotoxicity of FB1.Polygonatum sibiricum polysaccharides exhibit neuroprotective effects,but it is unclear whether they protect against FB1-induced neurotoxicity.Here,Caenorhabditis elegans(C.elegans)was used to study the alleviation effects of P.sibiricum polysaccharide extract(PSPE)preprotection on FB1-induced neurotoxicity.PSPE pretreatment improved the behavioral deficits and neuronal damage in FB1-induced C.elegans.In addition,20-200μg/mL PSPE alleviated the oxidative damage in FB1-induced C.elegans by regulating the levels of reactive oxygen species,antioxidant enzyme activities and gene expression.Further studies have shown that PSPE pretreatment induced mitochondrial expression,increased ATP levels and mitochondrial membrane potential,and regulated mitochondrial oxidative phosphorylation and dynamics.Together,the protective effects of PSPE against FB1-induced neurotoxicity might be closely related with the reduction of oxidative stress and the regulation of mitochondria.
文摘[Objective]This experiment aimed to study the extraction technology of Auricularia auricula polysaccharide（APS）.[Method] Polysaccharide yield was regarded as studying target.Based on the single factor experiment results,orthogonal experiment design was applied to study the effect of liquid-solid ratio,extraction time,extraction temperature on polysaccharide yield.[Result]The results showed that extraction temperature was an important factor that affects the yield of crude polysaccharide,the optimal technology condition of water extraction way was liquid-solid ratio of 1：70（g：ml）,4-hour extraction time with temperature at 100 ℃.[Conclusion] The yield of Auricularia auricular polysaccharide was 42.56% under the above mentioned condition.
基金Supported by The Key Research and Development Program of Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization(202141204)The 2024 Huanggang Municipal Level Science and Technology Innovation Special Fund(YBXM20240031).
文摘Morchella septimelata polysaccharide(MSP)possesses multiple biological activities.In this study,double enzyme method was used to extract polysaccharides from Morchella septimelata.The basis of single factor experiment and response surface design was used to optimize the extraction process of polysaccharides.The optimal extraction conditions of polysaccharide were as follows:extraction time 2 h,extraction temperature 40.5℃,solid-liquid ratio 1:15(w/v),and the amount of double enzyme(cellulase:β-glucanase,mass ratio 1:2)2.0%.Under these conditions,the extraction yield of polysaccharide reached(14.86±0.12)%.
基金supported by the National Natural Science Foundation of China(No.32202008 and 32302133).
文摘This study aimed to evaluate the feasibility of extracting polysaccharides with significant in vitro anti-aging activity from turmeric by-products.Turmeric polysaccharides were produced through hydrothermal extraction,ethanol precipitation,and purification,with a total sugar content of 88.50%(w/w)and a molecular weight of 5.409×105 Da.Structural analysis revealed that the polysaccharide chain was primarily composed of→4)-α-D-glucopyranose-(1→,→2)-α-L-rhamnopyranose-(1→,→6)-α-D-glucopyranose-(1→,T-α-L-arabinofuranose-(1→,T-α-D-glucopyranose-(1→,and T-β-D-glucopyranose-(1→.In the H2O2-induced senescence model,turmeric polysaccharides greatly inhibited cell apoptosis,reduced SA-β-galactosidase activity,and downregulated the expression of cyclin-dependent kinase inhibitor 2A(p16)and tumor protein p53(p53)in the p53/cyclindependent kinase inhibitor 1A(p21)/p16 signaling pathway,with the dose of 0.05 mg/mL showing the most pronounced effects.Taken together,turmeric polysaccharides can serve as a promising anti-aging agent in functional foods and pharmaceuticals,and this study will contribute to the value-added utilization of turmeric by-products.
基金Science and Technology Department of Sichuan Province[No.RZ2000000727]the Graduate Student Foundation for the Xihua Universities[No.YCJJ2018057].We thank Bullet Edits Limited for the linguistic editing and proofreading of the manuscript.
文摘Cordyceps militaris,a medicinal and edible fungus,has garnered significant interest for its polysaccharides,known for their diverse physiological activities and health benefits.The purpose of this study was to extract polysaccharides(CM-1)from Cordyceps militaris fruiting bodies using hot water extraction and column chromatography,and evaluate its structural characteristics,antioxidant capacity,and in vivo absorption effects.Additionally,the study aimed to assess its protective effects against carbon tetrachloride(CCl4)induced liver injury in mice.The results showed that CM-1 significantly decreased the expression levels of serum ALT,AST,TNF-α,IL-6,and IL-1β.Pre-treatment with CM-1 also increased the activities of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px).Furthermore,histopathological examination of liver tissues confirmed the protective effects of CM-1 against acute liver injury induced by CCl4.These results indicate CM-1 promising potential as a dietary supplement for liver injury prevention and treatment.
基金Supported by National Edible Fungus Industry Technology System(CARS-20)。
文摘Dictyophora rubrovolvata has beautiful shape,good taste and high nutritional value,and has anti-oxidation,anti-tumor,hypoglycemic and other effects.In this review,the biological characteristics,nutritive chemical composition,polysaccharide extraction and functions,cultivation and pest control of D.rubrovolvata were intensively discussed and summarized,and the future research direction was also prospected.This study aims to provide future recommendations for the research of D.rubrovolvata and then promote the development of D.rubrovolvata industry.
