Commercial tissue adhesives have been widely applied in wound hemostats and dressings while enhancing the hemostasis and healing capabilities is challenging to meet clinical needs.Herein,we designed the glucose-and ca...Commercial tissue adhesives have been widely applied in wound hemostats and dressings while enhancing the hemostasis and healing capabilities is challenging to meet clinical needs.Herein,we designed the glucose-and catechol-functionalized derivatives from commercialε-polylysine(EPL)and prepared the hydrogels by simple amidation and catechol-crosslinking reactions,which have larger swelling ratios of 220%–240%,suitable microporous size of about 6–8µm,and tissue adhesion strength of about 20–40 kPa.The hemolysis,cytotoxicity,and cellular double-staining assays indicate that those hydrogels had good biocompatibility and the H-3 hydrogel with higher glucose content gave a lower hemolysis ratio of 0.73%±0.14%.The blood-clotting index,blood cell attachment and adhesion studies showed those hydrogels had fast blood-coagulation,resulting in excellent hemostasis performance with a short hemostatic time of 38–46 s and less blood loss of 19%–34%in a liver hemorrhage model.A full-thickness rat-skin defect model further demonstrates that the H-3 hydrogel achieved fast wound healing with a wound closure of 70.0%±2.7%on postoperative day 7 and nearly full closure on day 14.Remarkably,the hydroproline level that denotes the collagen production reached a higher one of 7.24±0.55µg/mg comparable to that in normal skins on day 14,evidencing the wound healing was close to completion in the H-3 treatment.Consequently,this work provides a simple method to construct a glycosylated and catechol-functionalized hydrogel platform from commercial EPL,holding translational potentials in wound hemostats and dressings.展开更多
Polyethylenimine-poly（L-lysine） （PEI-PLL） copolymer was synthesized via ring-opening polymerization of L-lysine N-carboxyanhydride （Lys（Z）-NCA） initiated by PEI. The complexation of PEI-PLL with siRNA was stud...Polyethylenimine-poly（L-lysine） （PEI-PLL） copolymer was synthesized via ring-opening polymerization of L-lysine N-carboxyanhydride （Lys（Z）-NCA） initiated by PEI. The complexation of PEI-PLL with siRNA was studied by particle size and zeta potential measurements. The flow cytometric analysis and confocal imaging showed its excellent intracellular trafficking ability. PEI-PLL displayed higher gene silencing efficiency and lower cytotoxicity than commercial PEI-25k in vitro. In the antitumor study, PEI-PLL was further combined with siVEGF and showed obviously tumor inhibition effect for the treatment of CT26 tumor model. Therefore, PEI-PLL is a promising siRNA carrier candidate for further antitumor treatment in vivo.展开更多
Chirality is a key factor in the biological activity of many biomolecules. Poly（L-lysine）（PLL）, a polypeptide synthesized from L-lysine, is one of the mostly used cationic polymers for gene delivery. The effect of...Chirality is a key factor in the biological activity of many biomolecules. Poly（L-lysine）（PLL）, a polypeptide synthesized from L-lysine, is one of the mostly used cationic polymers for gene delivery. The effect of chirality of polylysine（PL） on its gene delivery remains unknown. Herein, we prepared three polylysines（PLs） with the similar molecular weight but different backbone chiralities including poly（L-lysine）（PLL）, poly（D-lysine）（PDL） and poly（DL-lysine）（PDLL）. The side chains of each PL were modified with propylene oxide（PO） of different chiralities including（R）PO,（S）PO and（R,S）PO. These PL-POs with distinct chirality in main and side chains could condense p DNA into polyplexes. The polyplexes had approximately the same size, zeta potential and binding ability, but showed distinct gene transfection efficiency. We found that the PLs of L-configuration in the main chain had higher transfection efficiency than that of D or DL configuration due to their faster cellular uptake, while the side chain chirality had no effect on transfection efficiency.展开更多
The ring-opening polymerization (ROP) of N-(tetrafluoroboran ammonium)-L-lysine-N-carboxyanhydride (NH3BF4-Lys NCA) was initiated by triethylamine to prepare hyperbranched polylysine (HPlys) fluoroborate with narrow p...The ring-opening polymerization (ROP) of N-(tetrafluoroboran ammonium)-L-lysine-N-carboxyanhydride (NH3BF4-Lys NCA) was initiated by triethylamine to prepare hyperbranched polylysine (HPlys) fluoroborate with narrow polydispersity at a low temperature. Both mass spectroscopy analysis and DFT computation evidenced that the resultant cyclic dimer mainly initiated ROP as a secondary species according to normal amine mechanism. The hyperbranched polylysine in aqueous solution existed in a unimolecular state at low concentrations, but transformed into spherical micellar aggregates at a high concentration of 5 mg/mL, as characterized by dynamic laser scattering and transmission electron microscopy. By utilizing multivalent coordination interactions between hyperbranched polylysine and gold, the plasmonic gold nanoparticles (HPlys@Au NPs) were successfully prepared in aqueous solution, which elevated the solution by 23.1℃ upon 5 min of NIR laser irradiation (808 nm, 2 W/cm2).展开更多
Infections frequently occur after skin injuries,posing a significant challenge in current clinical care.Frequently changing dressings to minimize wound infections and adhesions results in large amounts of medical wast...Infections frequently occur after skin injuries,posing a significant challenge in current clinical care.Frequently changing dressings to minimize wound infections and adhesions results in large amounts of medical waste.Therefore,developing environmentally friendly multifunctional dressings has considerable application and translational significance.This study aimed to prepare a wound dressing with favorable antimicrobial properties and biosafety by grafting a natural antimicrobial peptide,polylysine,onto a traditional cotton textile dressing.The cotton textile dressing offers excellent moisture absorption and softness,while polylysine provides excellent biocompatibility,a broad antimicrobial spectrum,and high stability.Furthermore,both materials are natural and biodegradable,making them ideal for environmentally friendly wound dressings.展开更多
Cell transplantation is an effective strategy to improve the repair effect of nerve guide conduits(NGCs).However,problems such as low loading efficiency and cell anoikis undermine the outcomes.Microcarriers are effici...Cell transplantation is an effective strategy to improve the repair effect of nerve guide conduits(NGCs).However,problems such as low loading efficiency and cell anoikis undermine the outcomes.Microcarriers are efficient 3D cell culture scaffolds,which can also prevent cell anoikis by providing substrate for adhesion during transplantation.Here,we demonstrate for the first time microcarrier-based cell transplantation in peripheral nerve repair.We first prepared macroporous chitosan microcarriers(CSMCs)by the emulsion-phase separation method,and then decorated the CSMCs with polylysine(pl-CSMCs)to improve cell affinity.We then loaded the pl-CSMCs with adipose-derived stem cells(ADSCs)and injected them into electrospun polycaprolactone/chitosan NGCs to repair rat sciatic nerve defects.The ADSCs-laden pl-CSMCs effectively improved nerve regeneration as demonstrated by evaluation of histology,motor function recovery,electrophysiology,and gastrocnemius recovery.With efficient cell transplantation,convenient operation,and the multiple merits of ADSCs,the ADSCs-laden pl-CSMCs hold good potential in peripheral nerve repair.展开更多
Poly-L-lysine(PLL)is a water-soluble biopolymer consist of repeating unit of L-lysine.It displays intrinsic non-antigenicity,antibacterial property,biocompatibility,and biodegradability,and facile synthesis procedure,...Poly-L-lysine(PLL)is a water-soluble biopolymer consist of repeating unit of L-lysine.It displays intrinsic non-antigenicity,antibacterial property,biocompatibility,and biodegradability,and facile synthesis procedure,there-fore has attracted particular attention in various biomedical and pharmaceutical applications.Following a brief introduction to PLL synthesis,this review focuses on the significant advances of PLL and PLL-derived complexes utilized in cargo delivery,tissue adhesives,and antibacterial materials.Meanwhile,this review summarized the modification and optimization of PLL complexes for further clinical translational studies.The final section dis-cusses the challenges and opportunities of PLL-based complexes in the biomedical areas.展开更多
Medical devices-related infections pose a great threat to patients and cause an increased morbidity and mortality. Herein, we prepare an antibacterial composite(TPU-x) through blending medical grade thermoplastic poly...Medical devices-related infections pose a great threat to patients and cause an increased morbidity and mortality. Herein, we prepare an antibacterial composite(TPU-x) through blending medical grade thermoplastic polyurethane(TPU) and the complex(PL-DOSS) of ε-polylysine(ε-PL) and docusate sodium(DOSS). >99% reduction of colony forming unit(CFU) can be obtained in TPU-x composite films even at relatively low content of PL-DOSS, e.g. 0.13% for Methicillin resistant S. aureus(MRSA) and 0.5% for E. coli. The excellent antibacterial activity is mainly attributed to the formation of PL-DOSS nanoparticles that are uniformly dispersed in the TPU matrix with a size of ~100 nm. TPU-x composite films exhibit long-term stability in saline and good biocompatibility, and retain mechanical properties of TPU.展开更多
DTPA was covalently conjugated to the copolymer of L lysine and L tyrosine, then transchelated with Gd EDTA,the resultant polymer chelates were further reacted with 6 O bromoacetyl D galacto...DTPA was covalently conjugated to the copolymer of L lysine and L tyrosine, then transchelated with Gd EDTA,the resultant polymer chelates were further reacted with 6 O bromoacetyl D galactose or methyl lactobionyloxyacetate to give PLT(Gd DTPA) with galactose moiety as liver targeting group.These macromolecular MRI contrast agents were characterized by means of FT IR, ICP AES, elementary analyses and UV Vis spectrophotometry. Their in vitro relaxivity and liver targeting property in mice were also investigated.展开更多
Oligo-（lysine） n （n = 1-4） containing different numbers of lysine residues was used to induce the condensation of silicic acid to form silica particles in neutral silicate solution. It was found that the condensat...Oligo-（lysine） n （n = 1-4） containing different numbers of lysine residues was used to induce the condensation of silicic acid to form silica particles in neutral silicate solution. It was found that the condensation rate and the formation of silica particles are dependent on the number of lysine residues in an oligo-lysine. Oligo-lysine with more lysine residues can link more silicic acid together to form a matrix that promotes the effective aggregation of the condensed silica pieces to form large silica particles.展开更多
Cyclosporin A reacted with 4-benzoylbenzoic acid under ultraviolet radiation to give the conjugate of CaA-BBa.Then,the conjugate was linked to poly-L-lysine with the action of I-ethyl-3-(3-dimethylaminopropyl)-carbodi...Cyclosporin A reacted with 4-benzoylbenzoic acid under ultraviolet radiation to give the conjugate of CaA-BBa.Then,the conjugate was linked to poly-L-lysine with the action of I-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride to generate CsA immunogen.There were 13.1-17.4 molecules of CsA on each molecule of PL.whose average molecular weight was 40000.The coupling efficiency was fairly high and the constitution of CsA was maintained well.展开更多
It was found that microperoxidase-11 (MP-if) can undergo photoreduction at the bareroughened silver electrode. No photoreduction happens at the roughened silver electrodemodified with mercaptoundecanoic carboxylic aci...It was found that microperoxidase-11 (MP-if) can undergo photoreduction at the bareroughened silver electrode. No photoreduction happens at the roughened silver electrodemodified with mercaptoundecanoic carboxylic acid/poly-lysine. The photoreduction mechanismis discussed.展开更多
Novel strategies for the simultaneous and portable detection of multiple analytes are highly favorable for clinical diagnosis and healthcare.Conventional colorimetric enzyme-linked immunosorbent assay(ELISA)is a widel...Novel strategies for the simultaneous and portable detection of multiple analytes are highly favorable for clinical diagnosis and healthcare.Conventional colorimetric enzyme-linked immunosorbent assay(ELISA)is a widely used laboratory technique for medical diagnostics,quality control,and research applications.However,nonspecific absorption of proteins may lead to a reduction of functional sites,resulting in high background and low sensitivity in ELISA.Herein,we report a simple method of functionalization of poly(methyl methacrylate)(PMMA)with polylysine to be used as the microfluidic microplate substrate for enhanced ELISA,enabling rapid,ultrasensitive,and multiplexed detection of infectious diseases.FTIR and fluorescence microscopy characterization confirmed high amine densities on polylysine-modified PMMA surface,resulting in high detection sensitivity of the colorimetric ELISA on the PMMA microdevice.The ultrasensitive polylysine-modified microplate can immobilize protein within 20 min and results of the assay can be viewed by the naked eye or scanned through a simple desktop scanner for quantitative analysis within 90 min.A sandwich-type immunoassay for the rapid and sensitive detection of immunoglobulin G(IgG),hepatitis B surface antigen(HBsAg),and hepatitis B core antigen(HBcAg)was demonstrated as a proof-of-concept for multiplexed detection.The limits of detection(LOD)of 200.0 pg/mL for IgG,180.0 pg/mL for HBsAg,and 300.0 pg/mL for HBcAg were achieved,without any specialized equipment like a microplate reader.The surface-modified microchip exhibited about 10-fold higher sensitivity than traditional microplates.This surface-modified microplate has tremendous potential as a point-ofcare multiplexed testing platform for many applications ranging from clinical diagnosis to environmental monitoring,particularly in resource-limited settings.展开更多
Paclitaxel(PTX) is one of the most effective anticancer drugs for the treatment of various solid tumors, but its clinical use is limited by its poor solubility, low bioavailability, and severe systemic toxicity. Encap...Paclitaxel(PTX) is one of the most effective anticancer drugs for the treatment of various solid tumors, but its clinical use is limited by its poor solubility, low bioavailability, and severe systemic toxicity. Encapsulation of PTX in polymeric nanoparticles is used to overcome these problems but these micelles still need improvements in stability, pharmacokinetics, therapeutic efficacy, and safety profiles. In this study, we demonstrate a facile fabrication of a stable PTX-binding micelle made from poly(ethylene glycol)-block-dendritic polylysine, whose primary amines were reacted with phenethyl isothiocyanate(PEITC), a hydrophobic anticancer agent under clinical study. The amphiphilic conjugate(PEG-Gx-PEITC; Gx, the generation of the polylysine dendron) formed well-defined micelles whose core was composed of phenyl groups and thiourea groups binding PTX via π-π stacking and hydrogen bonding. Compared with the PTX-loaded poly(ethylene glycol)-block-poly(D,L-lactide)(PEGPDLLA/PTX) micelles in clinical use, PTX-loaded PEG-Gx-PEITC third-generation(PEG-G3-PEITC/PTX) micelles showed slowed blood clearance, enhanced tumor accumulation, and thus much improved in vivo therapeutic efficacy in both subcutaneous and orthotopic human breast cancer xenografts. Therefore, PEG-G3-PEITC is a promising drug delivery system for PTX in the treatment of breast cancer.展开更多
基金financially supported by National Key Research and Development Project of China (No. 2021YFB4001101)the National Natural Science Foundation (NSFC) of China (No.22075176)+1 种基金Natural Science Foundation of Shanghai (No.22ZR1429200)NSFC projects (Nos. 51833006, 82071160,81870806 and 81974152)
文摘Commercial tissue adhesives have been widely applied in wound hemostats and dressings while enhancing the hemostasis and healing capabilities is challenging to meet clinical needs.Herein,we designed the glucose-and catechol-functionalized derivatives from commercialε-polylysine(EPL)and prepared the hydrogels by simple amidation and catechol-crosslinking reactions,which have larger swelling ratios of 220%–240%,suitable microporous size of about 6–8µm,and tissue adhesion strength of about 20–40 kPa.The hemolysis,cytotoxicity,and cellular double-staining assays indicate that those hydrogels had good biocompatibility and the H-3 hydrogel with higher glucose content gave a lower hemolysis ratio of 0.73%±0.14%.The blood-clotting index,blood cell attachment and adhesion studies showed those hydrogels had fast blood-coagulation,resulting in excellent hemostasis performance with a short hemostatic time of 38–46 s and less blood loss of 19%–34%in a liver hemorrhage model.A full-thickness rat-skin defect model further demonstrates that the H-3 hydrogel achieved fast wound healing with a wound closure of 70.0%±2.7%on postoperative day 7 and nearly full closure on day 14.Remarkably,the hydroproline level that denotes the collagen production reached a higher one of 7.24±0.55µg/mg comparable to that in normal skins on day 14,evidencing the wound healing was close to completion in the H-3 treatment.Consequently,this work provides a simple method to construct a glycosylated and catechol-functionalized hydrogel platform from commercial EPL,holding translational potentials in wound hemostats and dressings.
基金financially supported by the National Natural Science Foundation of China(Nos.51222307,51303173,51390480,21474104 and 51403205)the Ministry of Science and Technology of China(International cooperation and communication program 2011DFR51090)Jilin Province Science and Technology Development Program(Nos.20120306,20130521011JH)
文摘Polyethylenimine-poly（L-lysine） （PEI-PLL） copolymer was synthesized via ring-opening polymerization of L-lysine N-carboxyanhydride （Lys（Z）-NCA） initiated by PEI. The complexation of PEI-PLL with siRNA was studied by particle size and zeta potential measurements. The flow cytometric analysis and confocal imaging showed its excellent intracellular trafficking ability. PEI-PLL displayed higher gene silencing efficiency and lower cytotoxicity than commercial PEI-25k in vitro. In the antitumor study, PEI-PLL was further combined with siVEGF and showed obviously tumor inhibition effect for the treatment of CT26 tumor model. Therefore, PEI-PLL is a promising siRNA carrier candidate for further antitumor treatment in vivo.
基金financially supported by the National Basic Research Program(No.2014CB931900)the National Natural Science Foundation of China(No.21090352)
文摘Chirality is a key factor in the biological activity of many biomolecules. Poly（L-lysine）（PLL）, a polypeptide synthesized from L-lysine, is one of the mostly used cationic polymers for gene delivery. The effect of chirality of polylysine（PL） on its gene delivery remains unknown. Herein, we prepared three polylysines（PLs） with the similar molecular weight but different backbone chiralities including poly（L-lysine）（PLL）, poly（D-lysine）（PDL） and poly（DL-lysine）（PDLL）. The side chains of each PL were modified with propylene oxide（PO） of different chiralities including（R）PO,（S）PO and（R,S）PO. These PL-POs with distinct chirality in main and side chains could condense p DNA into polyplexes. The polyplexes had approximately the same size, zeta potential and binding ability, but showed distinct gene transfection efficiency. We found that the PLs of L-configuration in the main chain had higher transfection efficiency than that of D or DL configuration due to their faster cellular uptake, while the side chain chirality had no effect on transfection efficiency.
基金supported by the National Natural Science Foundation of China (No. 21474061)the Innovation Program ofShanghai Municipal Education Commission (No. 201701070002E0061)the Innovation Fund (No. IFPM2016B004) of Shanghaijiao Tong University & Affiliated Sixth People’s Hospital South Campus are appreciated
文摘The ring-opening polymerization (ROP) of N-(tetrafluoroboran ammonium)-L-lysine-N-carboxyanhydride (NH3BF4-Lys NCA) was initiated by triethylamine to prepare hyperbranched polylysine (HPlys) fluoroborate with narrow polydispersity at a low temperature. Both mass spectroscopy analysis and DFT computation evidenced that the resultant cyclic dimer mainly initiated ROP as a secondary species according to normal amine mechanism. The hyperbranched polylysine in aqueous solution existed in a unimolecular state at low concentrations, but transformed into spherical micellar aggregates at a high concentration of 5 mg/mL, as characterized by dynamic laser scattering and transmission electron microscopy. By utilizing multivalent coordination interactions between hyperbranched polylysine and gold, the plasmonic gold nanoparticles (HPlys@Au NPs) were successfully prepared in aqueous solution, which elevated the solution by 23.1℃ upon 5 min of NIR laser irradiation (808 nm, 2 W/cm2).
基金supported by the National Natural Science Foundation of China(Nos.82370977,52073186)Medical Science and Technology Project of Sichuan Provincial Health Commission(No.21PJ059)+2 种基金Science and Technology Application Fundamental Project of Sichuan Province(No.2022YFS0124)Funding for Distinguished Young Scholars of Sichuan Province(No.23NSFJQ0001)Strategic Cooperation Projects of Yi Bin City and Sichuan University(No.2020CDYB-6)。
文摘Infections frequently occur after skin injuries,posing a significant challenge in current clinical care.Frequently changing dressings to minimize wound infections and adhesions results in large amounts of medical waste.Therefore,developing environmentally friendly multifunctional dressings has considerable application and translational significance.This study aimed to prepare a wound dressing with favorable antimicrobial properties and biosafety by grafting a natural antimicrobial peptide,polylysine,onto a traditional cotton textile dressing.The cotton textile dressing offers excellent moisture absorption and softness,while polylysine provides excellent biocompatibility,a broad antimicrobial spectrum,and high stability.Furthermore,both materials are natural and biodegradable,making them ideal for environmentally friendly wound dressings.
基金National Natural Science Foundation of China(Grant No.51773018,51973018)Key Research and Development Projects of People’s Liberation Army(BWS17J036).
文摘Cell transplantation is an effective strategy to improve the repair effect of nerve guide conduits(NGCs).However,problems such as low loading efficiency and cell anoikis undermine the outcomes.Microcarriers are efficient 3D cell culture scaffolds,which can also prevent cell anoikis by providing substrate for adhesion during transplantation.Here,we demonstrate for the first time microcarrier-based cell transplantation in peripheral nerve repair.We first prepared macroporous chitosan microcarriers(CSMCs)by the emulsion-phase separation method,and then decorated the CSMCs with polylysine(pl-CSMCs)to improve cell affinity.We then loaded the pl-CSMCs with adipose-derived stem cells(ADSCs)and injected them into electrospun polycaprolactone/chitosan NGCs to repair rat sciatic nerve defects.The ADSCs-laden pl-CSMCs effectively improved nerve regeneration as demonstrated by evaluation of histology,motor function recovery,electrophysiology,and gastrocnemius recovery.With efficient cell transplantation,convenient operation,and the multiple merits of ADSCs,the ADSCs-laden pl-CSMCs hold good potential in peripheral nerve repair.
基金supported by the Natural Science Foundation of Jiangsu Province (BK20210016)the National Key R&D Program of China (2020YFA0710800)+2 种基金the Key Program of National Natural Science Foundation of China (grant no.81930043)the Major International (Regional)Joint Research Project of China (grant no.81720108020)the Jiangsu Provincial Key Research and Development Program (BE2020621).
文摘Poly-L-lysine(PLL)is a water-soluble biopolymer consist of repeating unit of L-lysine.It displays intrinsic non-antigenicity,antibacterial property,biocompatibility,and biodegradability,and facile synthesis procedure,there-fore has attracted particular attention in various biomedical and pharmaceutical applications.Following a brief introduction to PLL synthesis,this review focuses on the significant advances of PLL and PLL-derived complexes utilized in cargo delivery,tissue adhesives,and antibacterial materials.Meanwhile,this review summarized the modification and optimization of PLL complexes for further clinical translational studies.The final section dis-cusses the challenges and opportunities of PLL-based complexes in the biomedical areas.
基金the National Natural Science Foundation of China(Nos.51773201 and 51973212)the Bureau of Science and Technology of Changchun(No.19SS005)+1 种基金the Department of Science and Technology of Jilin Province(No.20200301017RQ)the Joint Program of CAS-Jilin Province(No.2019SYHZ0002).
文摘Medical devices-related infections pose a great threat to patients and cause an increased morbidity and mortality. Herein, we prepare an antibacterial composite(TPU-x) through blending medical grade thermoplastic polyurethane(TPU) and the complex(PL-DOSS) of ε-polylysine(ε-PL) and docusate sodium(DOSS). >99% reduction of colony forming unit(CFU) can be obtained in TPU-x composite films even at relatively low content of PL-DOSS, e.g. 0.13% for Methicillin resistant S. aureus(MRSA) and 0.5% for E. coli. The excellent antibacterial activity is mainly attributed to the formation of PL-DOSS nanoparticles that are uniformly dispersed in the TPU matrix with a size of ~100 nm. TPU-x composite films exhibit long-term stability in saline and good biocompatibility, and retain mechanical properties of TPU.
文摘DTPA was covalently conjugated to the copolymer of L lysine and L tyrosine, then transchelated with Gd EDTA,the resultant polymer chelates were further reacted with 6 O bromoacetyl D galactose or methyl lactobionyloxyacetate to give PLT(Gd DTPA) with galactose moiety as liver targeting group.These macromolecular MRI contrast agents were characterized by means of FT IR, ICP AES, elementary analyses and UV Vis spectrophotometry. Their in vitro relaxivity and liver targeting property in mice were also investigated.
文摘Oligo-（lysine） n （n = 1-4） containing different numbers of lysine residues was used to induce the condensation of silicic acid to form silica particles in neutral silicate solution. It was found that the condensation rate and the formation of silica particles are dependent on the number of lysine residues in an oligo-lysine. Oligo-lysine with more lysine residues can link more silicic acid together to form a matrix that promotes the effective aggregation of the condensed silica pieces to form large silica particles.
基金supported by the fund of Sichuan Key Science and Technology(97012152501)
文摘Cyclosporin A reacted with 4-benzoylbenzoic acid under ultraviolet radiation to give the conjugate of CaA-BBa.Then,the conjugate was linked to poly-L-lysine with the action of I-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride to generate CsA immunogen.There were 13.1-17.4 molecules of CsA on each molecule of PL.whose average molecular weight was 40000.The coupling efficiency was fairly high and the constitution of CsA was maintained well.
文摘It was found that microperoxidase-11 (MP-if) can undergo photoreduction at the bareroughened silver electrode. No photoreduction happens at the roughened silver electrodemodified with mercaptoundecanoic carboxylic acid/poly-lysine. The photoreduction mechanismis discussed.
基金support from NIH/NIAID(R41AI162477)the U.S.NSF(IIP2122712 and CHE2216473)+7 种基金the Cancer Prevention and Research Institute of Texas(CPRIT,RP210165)the TTUHSC-UTEP Joint Seed Grant,and the AAFS Foundation Research Lucas grantthe prior financial support to our research from the NIH/NIAID(R21AI107415)the NIH/NIGMS(SC2GM105584)the NIH/NIMHD RCMI Pilot grant(5G12MD007593-22)the NIH Building Scholar Summer Sabbatical Award,the NSF(IIP1953841,IIP2052347,and DMR1827745)the DOT(CARTEEH)the Philadelphia Foundation,the Medical Center of the Americas Foundation(MCA)。
文摘Novel strategies for the simultaneous and portable detection of multiple analytes are highly favorable for clinical diagnosis and healthcare.Conventional colorimetric enzyme-linked immunosorbent assay(ELISA)is a widely used laboratory technique for medical diagnostics,quality control,and research applications.However,nonspecific absorption of proteins may lead to a reduction of functional sites,resulting in high background and low sensitivity in ELISA.Herein,we report a simple method of functionalization of poly(methyl methacrylate)(PMMA)with polylysine to be used as the microfluidic microplate substrate for enhanced ELISA,enabling rapid,ultrasensitive,and multiplexed detection of infectious diseases.FTIR and fluorescence microscopy characterization confirmed high amine densities on polylysine-modified PMMA surface,resulting in high detection sensitivity of the colorimetric ELISA on the PMMA microdevice.The ultrasensitive polylysine-modified microplate can immobilize protein within 20 min and results of the assay can be viewed by the naked eye or scanned through a simple desktop scanner for quantitative analysis within 90 min.A sandwich-type immunoassay for the rapid and sensitive detection of immunoglobulin G(IgG),hepatitis B surface antigen(HBsAg),and hepatitis B core antigen(HBcAg)was demonstrated as a proof-of-concept for multiplexed detection.The limits of detection(LOD)of 200.0 pg/mL for IgG,180.0 pg/mL for HBsAg,and 300.0 pg/mL for HBcAg were achieved,without any specialized equipment like a microplate reader.The surface-modified microchip exhibited about 10-fold higher sensitivity than traditional microplates.This surface-modified microplate has tremendous potential as a point-ofcare multiplexed testing platform for many applications ranging from clinical diagnosis to environmental monitoring,particularly in resource-limited settings.
基金supported by the National Natural Science Foundation of China (U1501243, 51603181)the National Basic Research Program (2014CB931900)+1 种基金the National Natural Science Foundation of China (51603181)the Fundamental Research Funds for the Central Universities (2016QNA4024) for financial support
文摘Paclitaxel(PTX) is one of the most effective anticancer drugs for the treatment of various solid tumors, but its clinical use is limited by its poor solubility, low bioavailability, and severe systemic toxicity. Encapsulation of PTX in polymeric nanoparticles is used to overcome these problems but these micelles still need improvements in stability, pharmacokinetics, therapeutic efficacy, and safety profiles. In this study, we demonstrate a facile fabrication of a stable PTX-binding micelle made from poly(ethylene glycol)-block-dendritic polylysine, whose primary amines were reacted with phenethyl isothiocyanate(PEITC), a hydrophobic anticancer agent under clinical study. The amphiphilic conjugate(PEG-Gx-PEITC; Gx, the generation of the polylysine dendron) formed well-defined micelles whose core was composed of phenyl groups and thiourea groups binding PTX via π-π stacking and hydrogen bonding. Compared with the PTX-loaded poly(ethylene glycol)-block-poly(D,L-lactide)(PEGPDLLA/PTX) micelles in clinical use, PTX-loaded PEG-Gx-PEITC third-generation(PEG-G3-PEITC/PTX) micelles showed slowed blood clearance, enhanced tumor accumulation, and thus much improved in vivo therapeutic efficacy in both subcutaneous and orthotopic human breast cancer xenografts. Therefore, PEG-G3-PEITC is a promising drug delivery system for PTX in the treatment of breast cancer.
