A rapid and simple method for the determination of potyhexamethylene biguanide （polyhexanide, PHMB） in liquid and gel-like pharmaceutical formulations by means of high performance liquid chromatography coupled to di...A rapid and simple method for the determination of potyhexamethylene biguanide （polyhexanide, PHMB） in liquid and gel-like pharmaceutical formulations by means of high performance liquid chromatography coupled to diode-array detection （HPLC DAD） was devel- oped. Best separation was achieved using a cyanopropyl bonded phase （Agilent Zorbax Eclipse XDB-CN column 4.6mm x 75 mm with particle size of 3.5 μm） as well as gradient elution consisting of acetonitrile/deionized water at a flow rate of 1.0 mL/min. The optimized and applied chromatographic conditions permitted separation of polyhexanide from interacting raatrix with subsequent detection at a wavelength of 235 nm with good sensitivity. The method validation was carried out with regard to the guidelines for analytical procedures demanded by the International Conference on Harmonisation （ICH）. Mean recoveries of 102% and 101% for gel-like as well as liquid preparations were obtained. Suitable repeatability as well as intermediate precision could bc achieved with limits of detection ≤0.004 mg/mL for both formulations, equivalent to ≤0.004% PHMB concerning sample preparation. Determination of PHMB was accomplished without tedious sample preparation. Interacting matrix could be eliminated by the chromatographicprocedure with excellent performance of system suitability. All analytical requirements were fulfilled permitting a reliable and precise determination of PHMB in pharmaceuticals. Further- more, the developed method was applied to stability testing of pharmaceutical preparations containing PHMB.展开更多
文摘A rapid and simple method for the determination of potyhexamethylene biguanide （polyhexanide, PHMB） in liquid and gel-like pharmaceutical formulations by means of high performance liquid chromatography coupled to diode-array detection （HPLC DAD） was devel- oped. Best separation was achieved using a cyanopropyl bonded phase （Agilent Zorbax Eclipse XDB-CN column 4.6mm x 75 mm with particle size of 3.5 μm） as well as gradient elution consisting of acetonitrile/deionized water at a flow rate of 1.0 mL/min. The optimized and applied chromatographic conditions permitted separation of polyhexanide from interacting raatrix with subsequent detection at a wavelength of 235 nm with good sensitivity. The method validation was carried out with regard to the guidelines for analytical procedures demanded by the International Conference on Harmonisation （ICH）. Mean recoveries of 102% and 101% for gel-like as well as liquid preparations were obtained. Suitable repeatability as well as intermediate precision could bc achieved with limits of detection ≤0.004 mg/mL for both formulations, equivalent to ≤0.004% PHMB concerning sample preparation. Determination of PHMB was accomplished without tedious sample preparation. Interacting matrix could be eliminated by the chromatographicprocedure with excellent performance of system suitability. All analytical requirements were fulfilled permitting a reliable and precise determination of PHMB in pharmaceuticals. Further- more, the developed method was applied to stability testing of pharmaceutical preparations containing PHMB.
