Medical cotton dressing is cheap and widely used in diversified fields,but in the application of promoting wound healing,the continuous research of multifunctional medical cotton dressing is still of great significanc...Medical cotton dressing is cheap and widely used in diversified fields,but in the application of promoting wound healing,the continuous research of multifunctional medical cotton dressing is still of great significance.Here,we developed a fresh type of antibacterial cotton dressing through a succinct strategy based on chemically anchoring polyhexamethylene biguanide(PHMB).Intriguingly,after PHMB modification,the cotton dressing exhibited outstanding antibacterial performance which could maintain>99.99%antibacterial rate after several treatments,including washing 50 times,repeated use 10 times,UV irradiation for 7 days,cationic dyes dying,and conditioned under 90℃water bath for 2 h.In addition,the water contact angle of cotton dressing increased dramatically from 0°to 111°,which could facilitate bacterial adhesion,thus further enhance the antibacterial efficiency,and easily remove the bacterial debris.Apart from that,the developed cotton dressing showed good cytocompatibility,promoted blood clotting and expression of platelets,and promoted the wound healing process in the infection intervened skin wound model.Taken together,this antibacterial cotton dressing with desirable blood clotting,sustained protection against bacterial infection and bacterial removal features shows the potential to be a candidate for infected skin wound healing.展开更多
A rapid and simple method for the determination of potyhexamethylene biguanide （polyhexanide, PHMB） in liquid and gel-like pharmaceutical formulations by means of high performance liquid chromatography coupled to di...A rapid and simple method for the determination of potyhexamethylene biguanide （polyhexanide, PHMB） in liquid and gel-like pharmaceutical formulations by means of high performance liquid chromatography coupled to diode-array detection （HPLC DAD） was devel- oped. Best separation was achieved using a cyanopropyl bonded phase （Agilent Zorbax Eclipse XDB-CN column 4.6mm x 75 mm with particle size of 3.5 μm） as well as gradient elution consisting of acetonitrile/deionized water at a flow rate of 1.0 mL/min. The optimized and applied chromatographic conditions permitted separation of polyhexanide from interacting raatrix with subsequent detection at a wavelength of 235 nm with good sensitivity. The method validation was carried out with regard to the guidelines for analytical procedures demanded by the International Conference on Harmonisation （ICH）. Mean recoveries of 102% and 101% for gel-like as well as liquid preparations were obtained. Suitable repeatability as well as intermediate precision could bc achieved with limits of detection ≤0.004 mg/mL for both formulations, equivalent to ≤0.004% PHMB concerning sample preparation. Determination of PHMB was accomplished without tedious sample preparation. Interacting matrix could be eliminated by the chromatographicprocedure with excellent performance of system suitability. All analytical requirements were fulfilled permitting a reliable and precise determination of PHMB in pharmaceuticals. Further- more, the developed method was applied to stability testing of pharmaceutical preparations containing PHMB.展开更多
The massive use of polyhexamethylene guanidine(PHMG),as a typical bactericidal agent,raised environmental concerns to the public.This work comprehensively revealed the hormesis effects of PHMG occurred in waste activa...The massive use of polyhexamethylene guanidine(PHMG),as a typical bactericidal agent,raised environmental concerns to the public.This work comprehensively revealed the hormesis effects of PHMG occurred in waste activated sludge(WAS)on the generation of volatile fatty acids(VFAs)during anaerobic fermentation.The low level of PHMG(100 mg/g TSS)significantly promoted the VFAs generation(1283 mg COD/L,compared with 337 mg COD/L in the control)via synchronously facilitating the solubilization,hydrolysis,and acidification steps but inhibiting methanogenesis.Metagenomic analysis showed that the functional anaerobe(i.e.,Bacteroides,Macellibacteroide and Parabacteroide)and corresponding genetic expressions responsible for extracellular hydrolysis(i.e.,clp P),membrane transport(i.e.,ffh and gsp F),intracellular substrates metabolism(i.e.,ald and paa F)and VFAs biosynthesis(i.e.,ACACA and FASN)were enhanced in the optimal presence of PHMG.Moreover,the anaerobic species could respond and adapt to low PHMG stimuli via quorum sensing(i.e.,cqs A,rpf C and rpf G),and thus maintain the high microbial metabolic activities.However,they were unable to tolerate the toxicity of excessive PHMG,resulting in the extremely low VFAs production.This work enlightened the effects of emerging pollutants on WAS fermentation at the genetic levels,and provided guidance on the WAS treatment and resource recovery.展开更多
Although the PHMG （polyhexamethylene guanidine） and other oligomer guanidines are known as highly efficient biocides against a broad spectrum of microorganisms and eukaryotic cells, the cell protection by PHMG deriv...Although the PHMG （polyhexamethylene guanidine） and other oligomer guanidines are known as highly efficient biocides against a broad spectrum of microorganisms and eukaryotic cells, the cell protection by PHMG derivatives has been established firstly in this study. The antiviral protection was also exhibited after 15 min pretreatment of different cell cultures with low-concentration of PHMG salts. Monolayers of the continuous bovine tracheal cells culture （TCC） and primary culture of chicken embryo fibroblasts （FCE） were treated with aqueous solutions of PHMG chloride salts or PHMG succinate. The molecules of PHMG polycation adhered to the plasma membrane of the cells tested as they were treated with PHMG for 15-30 min. The viral material was added to the cell cultures after the wash-out carried out twice to rid of unbound PHMG. The viruses of Equine herpesvirus type 1, Rhinotracheitis infectious bovine and Equine infectious anemia virus were used. The protective effect from the cytopathic action of herpes and retroviruses was exhibited after 15 min pretreatment of cell monolayer with PHMG chloride at the TCC concentrations of 10^-3 - 10^-2% and FCE concentrations of 10^-5 - 10^-4%. The unique antiviral properties of PHMG salts represented in our research had never been shown before.展开更多
In this study,we used citric acid(CA)as a crosslinking agent,mixed with polyhexamethylene biguanide,to perform a pad-dry-cure treatment on wool fabrics to study its antimicrobial effects and physical properties.
In burn treatments,microorganisms on pressure garments during pressure therapy can prevent rehabilitation by causing functional,hygienic,and aesthetic difficulties. As bacterium is one of the most trouble-causing orga...In burn treatments,microorganisms on pressure garments during pressure therapy can prevent rehabilitation by causing functional,hygienic,and aesthetic difficulties. As bacterium is one of the most trouble-causing organisms,they can threaten patients causing infection during the long period of use of these garments.Novel burn pressure garments having durable antimicrobial property were developed using polyhexamethylene biguanide( PHMB)antimicrobial agent procedure on highly elastic nylon 66 /spandex fabrics in powernet,flat warp and weft knitted structures using paddry-cure method. Commercial wireless pressure sensors were used to control pressures at an acceptable medical range. Antimicrobial activity,wash durability,Fourier transform infrared spectroscopy(FTIR) and Scanning electron microscopy(SEM) analyses were conducted for the treated samples. Antimicrobial test results following AATCC 100 Test Method showed 99% reduction of bacteria for the fabric samples treated with PHMB. A small but significant decrease in antimicrobial activity was observed even after50 launderings. These treatments also yield good results to prevent odor,decrease infection by preventing and /or blocking microbial growth according to the antimicrobial mechanism and support reducing of scarring by providing a hygienic environment around the scar.展开更多
Vaginitis is one of the most recurrent conditions that afflict women and require medical care. Vulvovaginal candidiasis (VVC) is frequently related to vaginal carriage of Candida albicans, an opportunistic polymorphic...Vaginitis is one of the most recurrent conditions that afflict women and require medical care. Vulvovaginal candidiasis (VVC) is frequently related to vaginal carriage of Candida albicans, an opportunistic polymorphic fungus, which colonizes the human skin, the mucosa and the reproductive tract. Actually, standard treatments to counteract vaginitis include several topical and oral treatments. The aim of this study was to evaluate the efficacy of a new biguanide compound, polyhexamethylene biguanide (PHMB) in avaginal solution, in women affected by vaginal candidiasis. For this reason, 40 women showing the symptoms previously ascribed to vaginal candidiasis were recruited and treated with a single PHMB dose of vaginal solution. After a single dose of treatment, 80% of them had a complete resolution of the fungal infection;furthermore, all of them reported clinical benefits, statistically significant reduction in both clinical signs and symptoms of candidiasis and a score’s reduction of 50%.展开更多
Background:Hard-to-heal wounds are often compromised by the presence of biofilm.This presents an infection risk,yet traditional antimicrobial wound care products and systemic antibiotics are often used despite the unc...Background:Hard-to-heal wounds are often compromised by the presence of biofilm.This presents an infection risk,yet traditional antimicrobial wound care products and systemic antibiotics are often used despite the uncertainty of therapeutic success and wound progression.The aim of this study was to investigate the clinical impact of a next-generation anti-biofilm Hydrofiber wound dressing(AQUACEL Ag+Extra[AQAg+E])in hard-to-heal wounds that had previously been treated unsuccessfully with traditional silver-,iodine-or polyhexamethylene biguanide(PHMB)-containing dressings and products and/or systemic antibiotics.Methods:Clinical case study evaluations of the anti-biofilm dressing were conducted,where deteriorating or stagnant wounds were selected by clinicians and primary dressings were replaced by the anti-biofilm dressing for up to 4 weeks,or as deemed clinically appropriate,with monitoring via case report forms.The data was stratified for cases where traditional silver-,iodine-or PHMBcontaining products,or systemic antibiotics,had been used prior to the introduction of the antibiofilm dressing.Results:Sixty-five cases were identified for inclusion,wounds ranging in duration from 1 week to 20 years(median:12 months).In 47(72%)cases the wounds were stagnant,while 15(23%)were deteriorating;3 wounds were not recorded.After an average of 4.2 weeks of management with the anti-biofilm dressing(range:1–11 weeks),in 11(17%)cases the wounds had healed(i.e.complete wound closure),40(62%)wounds improved,9(14%)wounds remained the same and 5(8%)wounds deteriorated.Conclusions:The introduction of this anti-biofilm dressing into protocols of care that had previously involved wound management with traditional antimicrobial products and/or antibiotics was shown to facilitate improvements in the healing status of most of these hard-to-heal wounds.Dressings containing proven anti-biofilm technology,in combination with antimicrobial silver and exudate management technology,appear to be an effective alternative to traditional antimicrobial products and antibiotics in the cases presented here.The use of antimicrobial wound dressings that contain anti-biofilm technology may have a key role to play in more effective wound management and antibiotic stewardship.展开更多
基金the National Natural Science Foundation of China(Nos.51803128,52073186,21802097)Fundamental Research Funds for the Central Universities(No.20826041D4160)+2 种基金Sichuan Science and Technology Programs(Nos.2020KJT0031-2020ZHCG0051,2020KJT0061-2020ZHFP0148)State Key Laboratory of Polymer Materials Engineering(No.sklpme20213–01)Funding of Engineering Characteristic Team,Sichuan University(No.2020SCUNG122)。
文摘Medical cotton dressing is cheap and widely used in diversified fields,but in the application of promoting wound healing,the continuous research of multifunctional medical cotton dressing is still of great significance.Here,we developed a fresh type of antibacterial cotton dressing through a succinct strategy based on chemically anchoring polyhexamethylene biguanide(PHMB).Intriguingly,after PHMB modification,the cotton dressing exhibited outstanding antibacterial performance which could maintain>99.99%antibacterial rate after several treatments,including washing 50 times,repeated use 10 times,UV irradiation for 7 days,cationic dyes dying,and conditioned under 90℃water bath for 2 h.In addition,the water contact angle of cotton dressing increased dramatically from 0°to 111°,which could facilitate bacterial adhesion,thus further enhance the antibacterial efficiency,and easily remove the bacterial debris.Apart from that,the developed cotton dressing showed good cytocompatibility,promoted blood clotting and expression of platelets,and promoted the wound healing process in the infection intervened skin wound model.Taken together,this antibacterial cotton dressing with desirable blood clotting,sustained protection against bacterial infection and bacterial removal features shows the potential to be a candidate for infected skin wound healing.
文摘A rapid and simple method for the determination of potyhexamethylene biguanide （polyhexanide, PHMB） in liquid and gel-like pharmaceutical formulations by means of high performance liquid chromatography coupled to diode-array detection （HPLC DAD） was devel- oped. Best separation was achieved using a cyanopropyl bonded phase （Agilent Zorbax Eclipse XDB-CN column 4.6mm x 75 mm with particle size of 3.5 μm） as well as gradient elution consisting of acetonitrile/deionized water at a flow rate of 1.0 mL/min. The optimized and applied chromatographic conditions permitted separation of polyhexanide from interacting raatrix with subsequent detection at a wavelength of 235 nm with good sensitivity. The method validation was carried out with regard to the guidelines for analytical procedures demanded by the International Conference on Harmonisation （ICH）. Mean recoveries of 102% and 101% for gel-like as well as liquid preparations were obtained. Suitable repeatability as well as intermediate precision could bc achieved with limits of detection ≤0.004 mg/mL for both formulations, equivalent to ≤0.004% PHMB concerning sample preparation. Determination of PHMB was accomplished without tedious sample preparation. Interacting matrix could be eliminated by the chromatographicprocedure with excellent performance of system suitability. All analytical requirements were fulfilled permitting a reliable and precise determination of PHMB in pharmaceuticals. Further- more, the developed method was applied to stability testing of pharmaceutical preparations containing PHMB.
基金financially supported by the National Natural Science Foundation of China(No.52070069)Jiangsu Province Natural Science Foundation(No.BK20211207)+3 种基金China Postdoctoral Science Foundation(No.2021M692423)Shanghai Post-doctoral Excellence Program(No.2020419)State Key Laboratory of Pollution Control and Resource Reuse Foundation(No.PCRRF20005)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China。
文摘The massive use of polyhexamethylene guanidine(PHMG),as a typical bactericidal agent,raised environmental concerns to the public.This work comprehensively revealed the hormesis effects of PHMG occurred in waste activated sludge(WAS)on the generation of volatile fatty acids(VFAs)during anaerobic fermentation.The low level of PHMG(100 mg/g TSS)significantly promoted the VFAs generation(1283 mg COD/L,compared with 337 mg COD/L in the control)via synchronously facilitating the solubilization,hydrolysis,and acidification steps but inhibiting methanogenesis.Metagenomic analysis showed that the functional anaerobe(i.e.,Bacteroides,Macellibacteroide and Parabacteroide)and corresponding genetic expressions responsible for extracellular hydrolysis(i.e.,clp P),membrane transport(i.e.,ffh and gsp F),intracellular substrates metabolism(i.e.,ald and paa F)and VFAs biosynthesis(i.e.,ACACA and FASN)were enhanced in the optimal presence of PHMG.Moreover,the anaerobic species could respond and adapt to low PHMG stimuli via quorum sensing(i.e.,cqs A,rpf C and rpf G),and thus maintain the high microbial metabolic activities.However,they were unable to tolerate the toxicity of excessive PHMG,resulting in the extremely low VFAs production.This work enlightened the effects of emerging pollutants on WAS fermentation at the genetic levels,and provided guidance on the WAS treatment and resource recovery.
文摘Although the PHMG （polyhexamethylene guanidine） and other oligomer guanidines are known as highly efficient biocides against a broad spectrum of microorganisms and eukaryotic cells, the cell protection by PHMG derivatives has been established firstly in this study. The antiviral protection was also exhibited after 15 min pretreatment of different cell cultures with low-concentration of PHMG salts. Monolayers of the continuous bovine tracheal cells culture （TCC） and primary culture of chicken embryo fibroblasts （FCE） were treated with aqueous solutions of PHMG chloride salts or PHMG succinate. The molecules of PHMG polycation adhered to the plasma membrane of the cells tested as they were treated with PHMG for 15-30 min. The viral material was added to the cell cultures after the wash-out carried out twice to rid of unbound PHMG. The viruses of Equine herpesvirus type 1, Rhinotracheitis infectious bovine and Equine infectious anemia virus were used. The protective effect from the cytopathic action of herpes and retroviruses was exhibited after 15 min pretreatment of cell monolayer with PHMG chloride at the TCC concentrations of 10^-3 - 10^-2% and FCE concentrations of 10^-5 - 10^-4%. The unique antiviral properties of PHMG salts represented in our research had never been shown before.
文摘In this study,we used citric acid(CA)as a crosslinking agent,mixed with polyhexamethylene biguanide,to perform a pad-dry-cure treatment on wool fabrics to study its antimicrobial effects and physical properties.
基金Financial support of this research was provided by NC State University College of Textilesthe Scientific and Technological Research Council of Turkey
文摘In burn treatments,microorganisms on pressure garments during pressure therapy can prevent rehabilitation by causing functional,hygienic,and aesthetic difficulties. As bacterium is one of the most trouble-causing organisms,they can threaten patients causing infection during the long period of use of these garments.Novel burn pressure garments having durable antimicrobial property were developed using polyhexamethylene biguanide( PHMB)antimicrobial agent procedure on highly elastic nylon 66 /spandex fabrics in powernet,flat warp and weft knitted structures using paddry-cure method. Commercial wireless pressure sensors were used to control pressures at an acceptable medical range. Antimicrobial activity,wash durability,Fourier transform infrared spectroscopy(FTIR) and Scanning electron microscopy(SEM) analyses were conducted for the treated samples. Antimicrobial test results following AATCC 100 Test Method showed 99% reduction of bacteria for the fabric samples treated with PHMB. A small but significant decrease in antimicrobial activity was observed even after50 launderings. These treatments also yield good results to prevent odor,decrease infection by preventing and /or blocking microbial growth according to the antimicrobial mechanism and support reducing of scarring by providing a hygienic environment around the scar.
文摘Vaginitis is one of the most recurrent conditions that afflict women and require medical care. Vulvovaginal candidiasis (VVC) is frequently related to vaginal carriage of Candida albicans, an opportunistic polymorphic fungus, which colonizes the human skin, the mucosa and the reproductive tract. Actually, standard treatments to counteract vaginitis include several topical and oral treatments. The aim of this study was to evaluate the efficacy of a new biguanide compound, polyhexamethylene biguanide (PHMB) in avaginal solution, in women affected by vaginal candidiasis. For this reason, 40 women showing the symptoms previously ascribed to vaginal candidiasis were recruited and treated with a single PHMB dose of vaginal solution. After a single dose of treatment, 80% of them had a complete resolution of the fungal infection;furthermore, all of them reported clinical benefits, statistically significant reduction in both clinical signs and symptoms of candidiasis and a score’s reduction of 50%.
文摘Background:Hard-to-heal wounds are often compromised by the presence of biofilm.This presents an infection risk,yet traditional antimicrobial wound care products and systemic antibiotics are often used despite the uncertainty of therapeutic success and wound progression.The aim of this study was to investigate the clinical impact of a next-generation anti-biofilm Hydrofiber wound dressing(AQUACEL Ag+Extra[AQAg+E])in hard-to-heal wounds that had previously been treated unsuccessfully with traditional silver-,iodine-or polyhexamethylene biguanide(PHMB)-containing dressings and products and/or systemic antibiotics.Methods:Clinical case study evaluations of the anti-biofilm dressing were conducted,where deteriorating or stagnant wounds were selected by clinicians and primary dressings were replaced by the anti-biofilm dressing for up to 4 weeks,or as deemed clinically appropriate,with monitoring via case report forms.The data was stratified for cases where traditional silver-,iodine-or PHMBcontaining products,or systemic antibiotics,had been used prior to the introduction of the antibiofilm dressing.Results:Sixty-five cases were identified for inclusion,wounds ranging in duration from 1 week to 20 years(median:12 months).In 47(72%)cases the wounds were stagnant,while 15(23%)were deteriorating;3 wounds were not recorded.After an average of 4.2 weeks of management with the anti-biofilm dressing(range:1–11 weeks),in 11(17%)cases the wounds had healed(i.e.complete wound closure),40(62%)wounds improved,9(14%)wounds remained the same and 5(8%)wounds deteriorated.Conclusions:The introduction of this anti-biofilm dressing into protocols of care that had previously involved wound management with traditional antimicrobial products and/or antibiotics was shown to facilitate improvements in the healing status of most of these hard-to-heal wounds.Dressings containing proven anti-biofilm technology,in combination with antimicrobial silver and exudate management technology,appear to be an effective alternative to traditional antimicrobial products and antibiotics in the cases presented here.The use of antimicrobial wound dressings that contain anti-biofilm technology may have a key role to play in more effective wound management and antibiotic stewardship.
