[Objectives]This study was conducted to isolate and identify the components from stems of Polyalthia plagioneura.[Methods]The compounds were isolated and purified by silica gel column,Sephadex LH-20,and C_(18) chromat...[Objectives]This study was conducted to isolate and identify the components from stems of Polyalthia plagioneura.[Methods]The compounds were isolated and purified by silica gel column,Sephadex LH-20,and C_(18) chromatography.Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.[Results]Five compounds were isolated and identified as:di(2-ethylhexyl)phthalate(1),cinnamic anhydride(2),phthalic acid(3),citric acid(4),and syringaldehyde(5).[Conclusions]All compounds were isolated from this plant for the first time.展开更多
Objective:To investigate the anticancer activity of Polyalthia evecta(P.evecta)(Pierre) Finet & Gagnep against human hepatoma cell line(HepG2).Methods:The anticancer activity was based on(a) the cytotoxicity again...Objective:To investigate the anticancer activity of Polyalthia evecta(P.evecta)(Pierre) Finet & Gagnep against human hepatoma cell line(HepG2).Methods:The anticancer activity was based on(a) the cytotoxicity against human hepatoma cells(HepG2) assessed using a neutral red assay and(b) apoptosis induction determined by evaluation of nuclei morphological changes after DAP1 staining.Preliminary phytochemical analysis of the crude extract was assessed by HPLC analysis.Results:The 50% ethanol-water crude leaf extract of P.evecta(EW-L) showed greater potential anticancer activity with high cytotoxicity[IC_(50)=(62.8±7.3)μg/mL]and higher selectivity in HepG2 cells than normal Vero cells[selective index(SI)=7.9].The SI of EW-L was higher than the positive control,melphalan(SI=1.6) and the apoptotic cells(46.4±2.6)%induced by EW-L was higher than the melphalan(41.6±2.1)%(P<0.05).The HPLC chromatogram of the EW-L revealed the presence of various kinds of polyphenolics and flavonoids in it.Conclusions: P.evecta is a potential plant with anticancer activity.The isolation of pure compounds and determination of the bioactivity of individual compounds will be further performed.展开更多
Objective:To evaluate the anticancer activity of the extract fraction of Polyalthia evecta(P. evecta)(Pierre) Finet & Gagnep and the synergistic anticancer effect of the extracts from P.evecta by using the ATR/FT-...Objective:To evaluate the anticancer activity of the extract fraction of Polyalthia evecta(P. evecta)(Pierre) Finet & Gagnep and the synergistic anticancer effect of the extracts from P.evecta by using the ATR/FT-IR spectroscopy.Methods:The 50%ethanol-water crude leaf extract of P.evecta(EW-L) was prepared and was further fractionated to isolate various fractions.The anticancer activity was investigated from cytotoxicity against HepG2 using a neutral red assay and apoptosis induction by evaluation of nuclei morphological changes after DAPI staining. Synergistic anticancer effects of the extracts from P.evecta were performed using the ATR/FTIR spectroscopy.Results:The result showed that the EW-L showed higher cytotoxicity and apoptosis induction in HepG2 cells than its fractionated extracts.The hexane extract exhibited higher cytotoxicity and apoptosis induction than the water extracts,but less than the EW-L. The combined water and hexane extracts apparently increased cytotoxicity and apoptosis induction.The%apoptotic cells induced by the extract mixture were increased about 2-fold compared to the single hexane extract Conclusions:The polar extract fraction is necessary for the anticancer activity of the non-polar extract fraction.The ATR/FT-IR spectra illustrates the physical interaction among the constituents in the extract mixture and reveals the presence of polyphenolic constituents in the EW-L,which might play a role for the synergistic anticancer effect.展开更多
Objective:To explore antiulcer and antimicrobial properties of methanolic extract of Polyalthia longifolia var.pendula.Methods:Gastroprotective potential of Polyalthia longifolia was studied on ethanol and ethanol/HCl...Objective:To explore antiulcer and antimicrobial properties of methanolic extract of Polyalthia longifolia var.pendula.Methods:Gastroprotective potential of Polyalthia longifolia was studied on ethanol and ethanol/HCl induced ulcers at 2 different doses(270 and 540 mg/kg/body weight). Antimicrobial efficacy of Polyalthia longifolia(25 mg/mL) was also studied against six gram positive,seven gram negative bacteria and five fungi by agar well diffusion method.Minimum inhibitory concentration was determined by agar well diffusion method in two fold serial dilution, in the range of 97-25 000μg/mL.Results:The reduction of ulcer index in Polyalthia longifolia treated animals was found to be statistically significant with respect to control animals.The Polyalthia longifolia exhibited ulcer protection activity in dose dependent manner and was also better than the standard.In antimicrobial activity,gram positive bacteria were more susceptible to Polyalthia longifolia than gram negative bacteria and fungal strains.Conclusions:Results obtained confirm the antiulcer and antimicrobial potential of the Polyalthia longifolia.展开更多
The methanol, acetone and 1,4-dioxan fractions of leaves of Polyalthia longifolia (Sonn.) Thw. were evaluated for antibacterial and antifungal activity. 91 clinically important strains were used for the study which we...The methanol, acetone and 1,4-dioxan fractions of leaves of Polyalthia longifolia (Sonn.) Thw. were evaluated for antibacterial and antifungal activity. 91 clinically important strains were used for the study which were both clinical isolates as well as identified strains. Piperacillin and gentamicin were used as standards for antibacterial assay, while nystatin and flucanazole were used as standards for antifungal assay. The antibacterial activity was more pronounced against gram positive bacterial and fungal strains. Poor activity was shown against gram negative bacterial strains studied.展开更多
A new benzosesquiterpenoid dimer, 3 ' ,3 ' ' ' -bispolycerasoidol (1), along with its monomer, polycerasoidol, were isolated from the dried leaves of Polyalthia cheliensis Hu. The structure of 1 was es...A new benzosesquiterpenoid dimer, 3 ' ,3 ' ' ' -bispolycerasoidol (1), along with its monomer, polycerasoidol, were isolated from the dried leaves of Polyalthia cheliensis Hu. The structure of 1 was established by spectroscopic methods.展开更多
Objective:To study the aphrodisiac potential of Polyalthia(P.)bullata in fowl.Methods:In this study,testosterone,as an indicator of the aphrodisiac potential of P.bullata,was investigated for its release from TM3 Leyd...Objective:To study the aphrodisiac potential of Polyalthia(P.)bullata in fowl.Methods:In this study,testosterone,as an indicator of the aphrodisiac potential of P.bullata,was investigated for its release from TM3 Leydig cells grown in vitro and in 4 fowls given capsules containing P.bullata at a dose of 10 mg in each capsule twice a day,for 50 days.In the latter in vivo evaluation,mating behaviours were additionally determined after the treated fowls were released to the individual hens,and their testes and liver were dissected for histological examinations.Blood drawn from the fowls was assessed for any changes in diagnostic parameters.Results:In the in vitro test(TM3 Leydig cells),P.bullata was able to increase testosterone to 0.48 nmol/L within 72 h of incubation,compared to the untreated control with only 0.18 nmol/L,i.e.,an increase of 170%.In the in vivo test,outcomes in the fowls dosed with P.bullata showed similar positive elevations of testosterone to(9.72±1.10)nmol/L in comparison to the controls that showed a level of only(4.05±0.84)nmol/L.Total frequencies of mating behaviours were observed(wing flapping,body shakes,crowing and beak pecking)to be 23 counts for the test compared to only 15 for the control fowls.Histological examination of the male reproductive organs provided evidence of testosterone boosting based on an observable increase in the activity at the seminiferous tubules of testis tissues without any damaging effects,compared to the controls.In the nine diagnostic blood parameters assessed,including alanine aminotransferase,aspartate aminotransferase,and gamma glutamyltransferase,none was remarkably elevated compared to the controls.The histological changes in the liver were not severe and mainly consisted of only localized moderate but recoverable obstructions and swellings of the vessels and tubules.Conclusions:P.bullata is able to boost testosterone both in vitro and in vivo,with no acute toxicities.展开更多
Herbal plants act as a significant source for discovering new compounds with potential therapeutic activities.Polyalthia longifolia,which is commonly known as an Indian mast tree,has various pharmacological properties...Herbal plants act as a significant source for discovering new compounds with potential therapeutic activities.Polyalthia longifolia,which is commonly known as an Indian mast tree,has various pharmacological properties,such as an anticancer,ulcer protective,hypoglycemic,hypotensive,a corrosion inhibitor,a bio-adsorbent,and few more.Moreover,it is known as false ashoka owing to its close resemblance with Saraca indica(ashoka tree).Various compounds have been reported from the extract of some parts of the plant,such as leaves,bark,root,and seeds.These extracts possess an ability to treat a number of human ailments,such as fever,ulcer,skin diseases,helminthiasis,and cardiac problems.Studies performed on the leave extract shows evidence that some compounds cause cell death in various cancer cell lines.The plant also has some biological applications,such as antibacterial,antiviral,and antimicrobial,which makes it clinically significant and useful.This review is an effort to explore and gather plant information in an organized manner.It reveals detailed information about the propagation,synonyms,vernaculars,varieties of plant,medicinal significance,ecology and distribution,botanical and ethnobotanical description,phytochemical constituents,and pharmacological activity of the plant.展开更多
基金Supported by Jiangxi Education Department Project(GJJ201533)University-level Project of Gannan Medical University(YB201902).
文摘[Objectives]This study was conducted to isolate and identify the components from stems of Polyalthia plagioneura.[Methods]The compounds were isolated and purified by silica gel column,Sephadex LH-20,and C_(18) chromatography.Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.[Results]Five compounds were isolated and identified as:di(2-ethylhexyl)phthalate(1),cinnamic anhydride(2),phthalic acid(3),citric acid(4),and syringaldehyde(5).[Conclusions]All compounds were isolated from this plant for the first time.
基金Partially supported by the Plant Genetics Conservation Projectunder the Royal Initiation of Her Royal Highness Prineess Maha Chakri Sirindhorn andresearch funding from Khon Kaen University
文摘Objective:To investigate the anticancer activity of Polyalthia evecta(P.evecta)(Pierre) Finet & Gagnep against human hepatoma cell line(HepG2).Methods:The anticancer activity was based on(a) the cytotoxicity against human hepatoma cells(HepG2) assessed using a neutral red assay and(b) apoptosis induction determined by evaluation of nuclei morphological changes after DAP1 staining.Preliminary phytochemical analysis of the crude extract was assessed by HPLC analysis.Results:The 50% ethanol-water crude leaf extract of P.evecta(EW-L) showed greater potential anticancer activity with high cytotoxicity[IC_(50)=(62.8±7.3)μg/mL]and higher selectivity in HepG2 cells than normal Vero cells[selective index(SI)=7.9].The SI of EW-L was higher than the positive control,melphalan(SI=1.6) and the apoptotic cells(46.4±2.6)%induced by EW-L was higher than the melphalan(41.6±2.1)%(P<0.05).The HPLC chromatogram of the EW-L revealed the presence of various kinds of polyphenolics and flavonoids in it.Conclusions: P.evecta is a potential plant with anticancer activity.The isolation of pure compounds and determination of the bioactivity of individual compounds will be further performed.
基金scholarly supported by The Office of the Higher Education Commission,Thailand.under the Strategic Scholarships for Thai Doctoral Degree Programs(CHE-PhD-THA-RG 3/2549)partially supported by the Plant Genetics Conservation Project under the Hoyal Initiation of Her Royal Highness Princess Maha Chakri Sirindhorn and research funding from Khon Kaen University(520123)
文摘Objective:To evaluate the anticancer activity of the extract fraction of Polyalthia evecta(P. evecta)(Pierre) Finet & Gagnep and the synergistic anticancer effect of the extracts from P.evecta by using the ATR/FT-IR spectroscopy.Methods:The 50%ethanol-water crude leaf extract of P.evecta(EW-L) was prepared and was further fractionated to isolate various fractions.The anticancer activity was investigated from cytotoxicity against HepG2 using a neutral red assay and apoptosis induction by evaluation of nuclei morphological changes after DAPI staining. Synergistic anticancer effects of the extracts from P.evecta were performed using the ATR/FTIR spectroscopy.Results:The result showed that the EW-L showed higher cytotoxicity and apoptosis induction in HepG2 cells than its fractionated extracts.The hexane extract exhibited higher cytotoxicity and apoptosis induction than the water extracts,but less than the EW-L. The combined water and hexane extracts apparently increased cytotoxicity and apoptosis induction.The%apoptotic cells induced by the extract mixture were increased about 2-fold compared to the single hexane extract Conclusions:The polar extract fraction is necessary for the anticancer activity of the non-polar extract fraction.The ATR/FT-IR spectra illustrates the physical interaction among the constituents in the extract mixture and reveals the presence of polyphenolic constituents in the EW-L,which might play a role for the synergistic anticancer effect.
基金University Grants Commission,New Delhi,India for providing financial support as Junior Research Fellow
文摘Objective:To explore antiulcer and antimicrobial properties of methanolic extract of Polyalthia longifolia var.pendula.Methods:Gastroprotective potential of Polyalthia longifolia was studied on ethanol and ethanol/HCl induced ulcers at 2 different doses(270 and 540 mg/kg/body weight). Antimicrobial efficacy of Polyalthia longifolia(25 mg/mL) was also studied against six gram positive,seven gram negative bacteria and five fungi by agar well diffusion method.Minimum inhibitory concentration was determined by agar well diffusion method in two fold serial dilution, in the range of 97-25 000μg/mL.Results:The reduction of ulcer index in Polyalthia longifolia treated animals was found to be statistically significant with respect to control animals.The Polyalthia longifolia exhibited ulcer protection activity in dose dependent manner and was also better than the standard.In antimicrobial activity,gram positive bacteria were more susceptible to Polyalthia longifolia than gram negative bacteria and fungal strains.Conclusions:Results obtained confirm the antiulcer and antimicrobial potential of the Polyalthia longifolia.
文摘The methanol, acetone and 1,4-dioxan fractions of leaves of Polyalthia longifolia (Sonn.) Thw. were evaluated for antibacterial and antifungal activity. 91 clinically important strains were used for the study which were both clinical isolates as well as identified strains. Piperacillin and gentamicin were used as standards for antibacterial assay, while nystatin and flucanazole were used as standards for antifungal assay. The antibacterial activity was more pronounced against gram positive bacterial and fungal strains. Poor activity was shown against gram negative bacterial strains studied.
文摘A new benzosesquiterpenoid dimer, 3 ' ,3 ' ' ' -bispolycerasoidol (1), along with its monomer, polycerasoidol, were isolated from the dried leaves of Polyalthia cheliensis Hu. The structure of 1 was established by spectroscopic methods.
基金funded by grants awarded by Malaysian Technological University Network(MTUN)with grant number of UIC191201grants awarded by Universiti Malaysia Pahang with grant numbers of PDU203209(product development grant)PGRS1903203(postgraduate grant).
文摘Objective:To study the aphrodisiac potential of Polyalthia(P.)bullata in fowl.Methods:In this study,testosterone,as an indicator of the aphrodisiac potential of P.bullata,was investigated for its release from TM3 Leydig cells grown in vitro and in 4 fowls given capsules containing P.bullata at a dose of 10 mg in each capsule twice a day,for 50 days.In the latter in vivo evaluation,mating behaviours were additionally determined after the treated fowls were released to the individual hens,and their testes and liver were dissected for histological examinations.Blood drawn from the fowls was assessed for any changes in diagnostic parameters.Results:In the in vitro test(TM3 Leydig cells),P.bullata was able to increase testosterone to 0.48 nmol/L within 72 h of incubation,compared to the untreated control with only 0.18 nmol/L,i.e.,an increase of 170%.In the in vivo test,outcomes in the fowls dosed with P.bullata showed similar positive elevations of testosterone to(9.72±1.10)nmol/L in comparison to the controls that showed a level of only(4.05±0.84)nmol/L.Total frequencies of mating behaviours were observed(wing flapping,body shakes,crowing and beak pecking)to be 23 counts for the test compared to only 15 for the control fowls.Histological examination of the male reproductive organs provided evidence of testosterone boosting based on an observable increase in the activity at the seminiferous tubules of testis tissues without any damaging effects,compared to the controls.In the nine diagnostic blood parameters assessed,including alanine aminotransferase,aspartate aminotransferase,and gamma glutamyltransferase,none was remarkably elevated compared to the controls.The histological changes in the liver were not severe and mainly consisted of only localized moderate but recoverable obstructions and swellings of the vessels and tubules.Conclusions:P.bullata is able to boost testosterone both in vitro and in vivo,with no acute toxicities.
文摘Herbal plants act as a significant source for discovering new compounds with potential therapeutic activities.Polyalthia longifolia,which is commonly known as an Indian mast tree,has various pharmacological properties,such as an anticancer,ulcer protective,hypoglycemic,hypotensive,a corrosion inhibitor,a bio-adsorbent,and few more.Moreover,it is known as false ashoka owing to its close resemblance with Saraca indica(ashoka tree).Various compounds have been reported from the extract of some parts of the plant,such as leaves,bark,root,and seeds.These extracts possess an ability to treat a number of human ailments,such as fever,ulcer,skin diseases,helminthiasis,and cardiac problems.Studies performed on the leave extract shows evidence that some compounds cause cell death in various cancer cell lines.The plant also has some biological applications,such as antibacterial,antiviral,and antimicrobial,which makes it clinically significant and useful.This review is an effort to explore and gather plant information in an organized manner.It reveals detailed information about the propagation,synonyms,vernaculars,varieties of plant,medicinal significance,ecology and distribution,botanical and ethnobotanical description,phytochemical constituents,and pharmacological activity of the plant.
