To obtain the certificate of airworthiness,it is essential to conduct a full-scale aircraft static test.During such test,accurate and comprehensive wing deformation measurement is crucial for assessing its strength,st...To obtain the certificate of airworthiness,it is essential to conduct a full-scale aircraft static test.During such test,accurate and comprehensive wing deformation measurement is crucial for assessing its strength,stiffness,and bearing capability.This paper proposes a novel and cost-effective videogrammetric method using multi-camera system to achieve the non-contact,highprecision,and 3D measurement of overall static deformation for the large-scale wing structure.To overcome the difficulties of making,carrying,and employing the large 2D or 3D target for calibrating the cameras with large field of view,a flexible stereo cameras calibration method combining 1D target and epipolar geometry is proposed.The global calibration method,aided by a total station,is employed to unify the 3D data obtained from various binocular subsystems.A series of static load tests using a 10-meter-long large-scale wing have been conducted to validate the proposed system and methods.Furthermore,the proposed method was applied to the practical wing deformation measurement of both wings with a wingspan of 33.6 m in the full-size civil aircraft static test.The overall 3D profile and displacement data of the tested wing under various loads can be accurately obtained.The maximum error of distance and displacement measurement is less than 4.5 mm within the measurement range of 35 m in all load cases.These results demonstrate that the proposed method achieves effective,high-accuracy,on-site,and visualized wing deformation measurement,making it a promising approach for full-scale aircraft wing static test.展开更多
The study of rock failure mechanisms is fundamental to geotechnical engineering,as it enhances design quality and mitigates disaster risks.This research employed in situ compression tests on 3D-printed rocklike sample...The study of rock failure mechanisms is fundamental to geotechnical engineering,as it enhances design quality and mitigates disaster risks.This research employed in situ compression tests on 3D-printed rocklike samples with a single flaw,combining Micro-CT scans and a specialized loading device to analyze their behavior.Mechanical properties and failure modes of these printed samples were compared to those of natural flawed sandstones,demonstrating the capability of 3D printing to replicate natural rock characteristics.By reconstructing 3D crack evolution from 2D CT images and applying digital volume correlation(DVC),the study visualized internal strain fields and established a relationship between strain patterns and rock failure.The results reveal that crack initiation consistently occurs at the flaw,advancing into tensile and secondary shear or mixed cracks.For flaw angles(α)ranging from 0°to 45°,the 3D-printed samples exhibited a higher number of newly formed cracks and a faster increase in crack volume with strain.In contrast,for flaw angles of 45°≤α≤90°,the opposite trend was observed.The internal strain field exhibited significant strain localization,with this uneven distribution playing a critical role in sample failure.When the flaw angle was in the range of 0°≤α≤30°,failure was primarily driven by tensile cracks,forming distinct tensile bands.Conversely,for 30°<α≤90°,a combination of tensile and shear cracks dominated the failure,producing both shear and tensile bands in the sample.Additionally,the strain field component ε_(yy) showed a strong correlation with the evolution of internal damage,providing valuable insights into the underlying rock failure mechanisms.展开更多
In this paper,some basic theories and terms of language testing,like types of language tests and qualities of good tests were briefly discussed.Then CET3(College English Test Band3) in Beijing was analyzed from three ...In this paper,some basic theories and terms of language testing,like types of language tests and qualities of good tests were briefly discussed.Then CET3(College English Test Band3) in Beijing was analyzed from three perspectives:design principles,item formats and test content. In the end,some suggestions were given to improve this test.展开更多
Part One:Quick Responses Please make a quick response to the sentence you will hear.1.I’m really sorry,but I can’t come to your party tonight.2.I’m so upset,Jean,I lost my bag on my way to the Science Museum.
Aim： To investigate wether the corresponding protein of mono-ADP-ribosyltransferase 3 （ART3） mRNA is expressed in human testes and, if so, whether the expression is cell type-specific. Methods： ART3 mRNA was deter...Aim： To investigate wether the corresponding protein of mono-ADP-ribosyltransferase 3 （ART3） mRNA is expressed in human testes and, if so, whether the expression is cell type-specific. Methods： ART3 mRNA was determined in human testes and sperm by reverse transcription-polymerase chain reaction （RT-PCR）. The glycosylphosphatidylinositol linkage of ART3 was shown by treating ART3-transfected HEK-293-T cells with phospholipase C. Fluorescent activated cell sorter （FACS）-analyses were used to detect ART3 on mature spermatozoa and immunohistological studies to detect the protein in testes. Results： ART3 protein was shown to be present in testes. It was found on spermatocytes only. It was absent from spermatogonia, spermatids and spermatozoa. The absence of ART3 from spermatozoa was confirmed by FACS-analysis. ART3 protein was detected neither within a seminoma nor on Leydig cells. Conclusion： Here we show for the first time that ART3 protein is expressed in testes in particular on spermatocytes, indicating that ART3 exerts a specific function only required at a particular stage of spermatogenesis.展开更多
Poly-3-hydroxybutyrate （PHB） can be produced by various species of bacteria. Among the possible carbon sources, both methane and methanol could be a suitable substrate for the production of PHB. Methane is cheap and...Poly-3-hydroxybutyrate （PHB） can be produced by various species of bacteria. Among the possible carbon sources, both methane and methanol could be a suitable substrate for the production of PHB. Methane is cheap and plentiful not only as natural gas, but also as biogas. Methanol can also maintain methanotrophic activity in some conditions. The methanotrophic strain Methylosinus trichosporium IMV3011 can accumulate PHB with methane and methanol in a brief nonsterile process. Liquid methanol （0.1%） was added to improve the oxidization of methane. The studies were carried out using shake flasks. Cultivation was performed in two stages： a continuous growth phase and a PHB accumulation phase under the conditions short of essential nutrients （ammonium, nitrate, phosphorus, copper, iron （Ⅲ）, magnesium or ethylenediamine tetraacetate （EDTA）） in batch culture. It was found that the most suitable growth time for the cell is 144 h. Then an optimized culture condition for second stage was determined, in which the PHB concentration could be much increased to 0.6 g/L. In order to increase PHB content, citric acid was added as an inhibitor of tricarboxylic acid cycle （TCA）. It was found that citric acid is favorable for the PHB accumulation, and the PHB yield was increased to 40% （w/w） from the initial yield of 12% （w/w） after nutrient deficiency cultivation. The PHB produced is of very high quality with molecular weight up to 1.5 × 10^6Da.展开更多
Biomimetic adsorbent named as PHBBMA was prepared from lipophilic poly-3-hydroxybutyrate （PHB） by a modified double emulsion solvent evaporation method. PHBBMA, characterized by using scanning electron microscope an...Biomimetic adsorbent named as PHBBMA was prepared from lipophilic poly-3-hydroxybutyrate （PHB） by a modified double emulsion solvent evaporation method. PHBBMA, characterized by using scanning electron microscope and nitrogen adsorption/desorption measurements, is porous spherical particles. The characterization with the thermal gravimetric analysis and differential scanning calorimetry, 1 H nuclear magnetic resonance and Fourier transform infrared spectroscopy showed that PHBBMA preparation was a physical process without chemical reaction. The adsorption of PHBBMA for o-nitrochlorobenzene （o-NCB） was fitted better by Langmuir model than by Freundlich model, while the pseudo second-order model fitting was better than the pseudo first-order model fitting. The maximal adsorption capacity of PHBBMA for o-NCB was 57.83 mg/g at 30°C, although its specific surface area （S BET ） was only 8.45 m 2 /g. PHBBMA is a safe and environmental friendly adsorbent with high adsorption capacity because its component is innocuous and biodegradable PHB produced reusing wastes and contaminants, no byproduct can produced, and its ester and hydrocarbyl groups have strong affinity with organochlorine compounds. The further work will focus on the modification and improvement of PHBBMA in order to increase its S BET and adsorption capacity.展开更多
Soil test for availability of nutrients and heavy metals is extensively served as a means for the evaluations of soil fertility, and environmental effects and phytotoxicity of pollutants in soils, and for the fertiliz...Soil test for availability of nutrients and heavy metals is extensively served as a means for the evaluations of soil fertility, and environmental effects and phytotoxicity of pollutants in soils, and for the fertilizer recommendation in agricultural and environmental sciences. Therefore, great attention has been paid to the measurement of elemental availability in soil test.展开更多
The double blade pump is widely used in sewage treatment industry,however,the research on the internal flow characteristics of the double blade pump with particle image velocimetry（PIV） technology is very little at ...The double blade pump is widely used in sewage treatment industry,however,the research on the internal flow characteristics of the double blade pump with particle image velocimetry（PIV） technology is very little at present.To reveal inner flow characteristics in double blade pump impeller under off-design and design conditions,inner flows in a double blade pump impeller,whose specific speed is 111,are measured under the five off-design conditions and design condition by using 3D PIV test technology.In order to ensure the accuracy of the 3D PIV test,the external trigger synchronization system which makes use of fiber optic and equivalent calibration method are applied.The 3D PIV relative velocity synthesis procedure is compiled by using Visual C＋＋ 2005.Then absolute velocity distribution and relative velocity distribution in the double blade pump impeller are obtained.Test results show that vortex exists in each condition,but the location,size and velocity of vortex core are different.Average absolute velocity value of impeller outlet increases at first,then decreases,and then increases again with increase of flow rate.Again average relative velocity values under 0.4,0.8,and 1.2 design condition are higher than that under 1.0 design condition,while under 0.6 and 1.4 design condition it is lower.Under low flow rate conditions,radial vectors of absolute velocities at impeller outlet and blade inlet near the pump shaft decrease with increase of flow rate,while that of relative velocities at the suction side near the pump shaft decreases.Radial vectors of absolute velocities and relative velocities change slightly under the two large flow rate conditions.The research results can be applied to instruct the hydraulic optimization design of double blade pumps.展开更多
Chang'E-3 probe incorporates four landing gears to assure the soft-landing,which are stowed while launching and deployed after separated from rocket. Deployment reliability is quite crucial for this mission. The d...Chang'E-3 probe incorporates four landing gears to assure the soft-landing,which are stowed while launching and deployed after separated from rocket. Deployment reliability is quite crucial for this mission. The deployment reliability test( DRT) method and assessment method were developed in this paper. Then DRT was conducted and the deployment reliability estimate of Chang'E-3probe was used to verify the proposed methods.展开更多
Landslide is the second largest natural disaster after earthquake. It is of significance to study the evolution laws and failure mechanism of landslides based on its surface 3D deformation information. Based on the ra...Landslide is the second largest natural disaster after earthquake. It is of significance to study the evolution laws and failure mechanism of landslides based on its surface 3D deformation information. Based on the rainfall-triggered waste dump instability model test, we studied the failure mechanisms of the waste dump by integrating surface deformation and internal slope stress and proposed novel parameters for identifying landslide stability. We developed a noncontact measurement device, which can obtain millimeter-level 3D deformation data for surface scene in physical model test;Then we developed the similar materials and established a test model for a waste dump. Based on the failure characteristics of slope surface, internal stress of slope body and displacement contours during the whole process, we divided the slope instability process in model test into four stages: rainfall infiltration and surface erosion, shallow sliding, deep sliding, and overall instability. Based on the obtained surface deformation data, we calculated the volume change during slope instability process and compared it with the point displacement on slope surface. The results showed that the volume change can not only reflect the slow-ultra acceleration process of slope failure, but also fully reflect the above four stages and reduce the fluctuations caused by random factors. Finally, this paper proposed two stability identification parameters: the volume change rate above the slip surface and the relative velocity of volume change rate. According to the calculation of these two parameters in model test, they can be used for study the deformation and failure mechanism of slope stability.展开更多
文摘To obtain the certificate of airworthiness,it is essential to conduct a full-scale aircraft static test.During such test,accurate and comprehensive wing deformation measurement is crucial for assessing its strength,stiffness,and bearing capability.This paper proposes a novel and cost-effective videogrammetric method using multi-camera system to achieve the non-contact,highprecision,and 3D measurement of overall static deformation for the large-scale wing structure.To overcome the difficulties of making,carrying,and employing the large 2D or 3D target for calibrating the cameras with large field of view,a flexible stereo cameras calibration method combining 1D target and epipolar geometry is proposed.The global calibration method,aided by a total station,is employed to unify the 3D data obtained from various binocular subsystems.A series of static load tests using a 10-meter-long large-scale wing have been conducted to validate the proposed system and methods.Furthermore,the proposed method was applied to the practical wing deformation measurement of both wings with a wingspan of 33.6 m in the full-size civil aircraft static test.The overall 3D profile and displacement data of the tested wing under various loads can be accurately obtained.The maximum error of distance and displacement measurement is less than 4.5 mm within the measurement range of 35 m in all load cases.These results demonstrate that the proposed method achieves effective,high-accuracy,on-site,and visualized wing deformation measurement,making it a promising approach for full-scale aircraft wing static test.
基金supported by the Korea Institute of Energy Technology Evaluation and Planning(KETEP)grant funded by the Korea Government(MOTIE)(Grant No.20214000000500,Training program of CCUS for the green growth)by the National Research Foundation of Korea(NRF)grant funded by the Korea Government(MSIT)(Grant No.2022R1F1A1076409)the support from the Chinese Scholarship Council for awarding a scholarship(CSC No.202106820011).
文摘The study of rock failure mechanisms is fundamental to geotechnical engineering,as it enhances design quality and mitigates disaster risks.This research employed in situ compression tests on 3D-printed rocklike samples with a single flaw,combining Micro-CT scans and a specialized loading device to analyze their behavior.Mechanical properties and failure modes of these printed samples were compared to those of natural flawed sandstones,demonstrating the capability of 3D printing to replicate natural rock characteristics.By reconstructing 3D crack evolution from 2D CT images and applying digital volume correlation(DVC),the study visualized internal strain fields and established a relationship between strain patterns and rock failure.The results reveal that crack initiation consistently occurs at the flaw,advancing into tensile and secondary shear or mixed cracks.For flaw angles(α)ranging from 0°to 45°,the 3D-printed samples exhibited a higher number of newly formed cracks and a faster increase in crack volume with strain.In contrast,for flaw angles of 45°≤α≤90°,the opposite trend was observed.The internal strain field exhibited significant strain localization,with this uneven distribution playing a critical role in sample failure.When the flaw angle was in the range of 0°≤α≤30°,failure was primarily driven by tensile cracks,forming distinct tensile bands.Conversely,for 30°<α≤90°,a combination of tensile and shear cracks dominated the failure,producing both shear and tensile bands in the sample.Additionally,the strain field component ε_(yy) showed a strong correlation with the evolution of internal damage,providing valuable insights into the underlying rock failure mechanisms.
文摘In this paper,some basic theories and terms of language testing,like types of language tests and qualities of good tests were briefly discussed.Then CET3(College English Test Band3) in Beijing was analyzed from three perspectives:design principles,item formats and test content. In the end,some suggestions were given to improve this test.
文摘Part One:Quick Responses Please make a quick response to the sentence you will hear.1.I’m really sorry,but I can’t come to your party tonight.2.I’m so upset,Jean,I lost my bag on my way to the Science Museum.
文摘Aim： To investigate wether the corresponding protein of mono-ADP-ribosyltransferase 3 （ART3） mRNA is expressed in human testes and, if so, whether the expression is cell type-specific. Methods： ART3 mRNA was determined in human testes and sperm by reverse transcription-polymerase chain reaction （RT-PCR）. The glycosylphosphatidylinositol linkage of ART3 was shown by treating ART3-transfected HEK-293-T cells with phospholipase C. Fluorescent activated cell sorter （FACS）-analyses were used to detect ART3 on mature spermatozoa and immunohistological studies to detect the protein in testes. Results： ART3 protein was shown to be present in testes. It was found on spermatocytes only. It was absent from spermatogonia, spermatids and spermatozoa. The absence of ART3 from spermatozoa was confirmed by FACS-analysis. ART3 protein was detected neither within a seminoma nor on Leydig cells. Conclusion： Here we show for the first time that ART3 protein is expressed in testes in particular on spermatocytes, indicating that ART3 exerts a specific function only required at a particular stage of spermatogenesis.
基金New Century Excellent Talents in University of China(NCET-05-0358)the National Natural Science Foundation of China(20625308)
文摘Poly-3-hydroxybutyrate （PHB） can be produced by various species of bacteria. Among the possible carbon sources, both methane and methanol could be a suitable substrate for the production of PHB. Methane is cheap and plentiful not only as natural gas, but also as biogas. Methanol can also maintain methanotrophic activity in some conditions. The methanotrophic strain Methylosinus trichosporium IMV3011 can accumulate PHB with methane and methanol in a brief nonsterile process. Liquid methanol （0.1%） was added to improve the oxidization of methane. The studies were carried out using shake flasks. Cultivation was performed in two stages： a continuous growth phase and a PHB accumulation phase under the conditions short of essential nutrients （ammonium, nitrate, phosphorus, copper, iron （Ⅲ）, magnesium or ethylenediamine tetraacetate （EDTA）） in batch culture. It was found that the most suitable growth time for the cell is 144 h. Then an optimized culture condition for second stage was determined, in which the PHB concentration could be much increased to 0.6 g/L. In order to increase PHB content, citric acid was added as an inhibitor of tricarboxylic acid cycle （TCA）. It was found that citric acid is favorable for the PHB accumulation, and the PHB yield was increased to 40% （w/w） from the initial yield of 12% （w/w） after nutrient deficiency cultivation. The PHB produced is of very high quality with molecular weight up to 1.5 × 10^6Da.
基金supported by the National Hi-Tech Research and Development Program (863) ofChina (No. 2006AA06Z378)the National Natural Science Foundation of China (No. 20777018, 20977035)+1 种基金the National Key Technology R&D Program of China(No. 2008BAC32B06-1)the Science and Technology Plan Project of Guangdong Province, China (No.2007B030103011)
文摘Biomimetic adsorbent named as PHBBMA was prepared from lipophilic poly-3-hydroxybutyrate （PHB） by a modified double emulsion solvent evaporation method. PHBBMA, characterized by using scanning electron microscope and nitrogen adsorption/desorption measurements, is porous spherical particles. The characterization with the thermal gravimetric analysis and differential scanning calorimetry, 1 H nuclear magnetic resonance and Fourier transform infrared spectroscopy showed that PHBBMA preparation was a physical process without chemical reaction. The adsorption of PHBBMA for o-nitrochlorobenzene （o-NCB） was fitted better by Langmuir model than by Freundlich model, while the pseudo second-order model fitting was better than the pseudo first-order model fitting. The maximal adsorption capacity of PHBBMA for o-NCB was 57.83 mg/g at 30°C, although its specific surface area （S BET ） was only 8.45 m 2 /g. PHBBMA is a safe and environmental friendly adsorbent with high adsorption capacity because its component is innocuous and biodegradable PHB produced reusing wastes and contaminants, no byproduct can produced, and its ester and hydrocarbyl groups have strong affinity with organochlorine compounds. The further work will focus on the modification and improvement of PHBBMA in order to increase its S BET and adsorption capacity.
文摘Soil test for availability of nutrients and heavy metals is extensively served as a means for the evaluations of soil fertility, and environmental effects and phytotoxicity of pollutants in soils, and for the fertilizer recommendation in agricultural and environmental sciences. Therefore, great attention has been paid to the measurement of elemental availability in soil test.
基金supported by National Outstanding Young Scientists Founds of China (Grant No. 50825902)National Key Technology R&D Program of China (Grant No. 2008BAF34B15)+2 种基金National Natural Science Foundation of China (Grant No. 51079062)Jiangsu Provincial 333 High-level Personal Training Project of ChinaJiangsu Provincial Six Talent Peak of China
文摘The double blade pump is widely used in sewage treatment industry,however,the research on the internal flow characteristics of the double blade pump with particle image velocimetry（PIV） technology is very little at present.To reveal inner flow characteristics in double blade pump impeller under off-design and design conditions,inner flows in a double blade pump impeller,whose specific speed is 111,are measured under the five off-design conditions and design condition by using 3D PIV test technology.In order to ensure the accuracy of the 3D PIV test,the external trigger synchronization system which makes use of fiber optic and equivalent calibration method are applied.The 3D PIV relative velocity synthesis procedure is compiled by using Visual C＋＋ 2005.Then absolute velocity distribution and relative velocity distribution in the double blade pump impeller are obtained.Test results show that vortex exists in each condition,but the location,size and velocity of vortex core are different.Average absolute velocity value of impeller outlet increases at first,then decreases,and then increases again with increase of flow rate.Again average relative velocity values under 0.4,0.8,and 1.2 design condition are higher than that under 1.0 design condition,while under 0.6 and 1.4 design condition it is lower.Under low flow rate conditions,radial vectors of absolute velocities at impeller outlet and blade inlet near the pump shaft decrease with increase of flow rate,while that of relative velocities at the suction side near the pump shaft decreases.Radial vectors of absolute velocities and relative velocities change slightly under the two large flow rate conditions.The research results can be applied to instruct the hydraulic optimization design of double blade pumps.
基金National Science and Technology Major Project,China
文摘Chang'E-3 probe incorporates four landing gears to assure the soft-landing,which are stowed while launching and deployed after separated from rocket. Deployment reliability is quite crucial for this mission. The deployment reliability test( DRT) method and assessment method were developed in this paper. Then DRT was conducted and the deployment reliability estimate of Chang'E-3probe was used to verify the proposed methods.
基金funded by the National Key R&D Program of China (Grant No. 2021YFB3901402)the Fundamental Research Funds for the Central Universities (Project No. 2022CDJKYJH037)。
文摘Landslide is the second largest natural disaster after earthquake. It is of significance to study the evolution laws and failure mechanism of landslides based on its surface 3D deformation information. Based on the rainfall-triggered waste dump instability model test, we studied the failure mechanisms of the waste dump by integrating surface deformation and internal slope stress and proposed novel parameters for identifying landslide stability. We developed a noncontact measurement device, which can obtain millimeter-level 3D deformation data for surface scene in physical model test;Then we developed the similar materials and established a test model for a waste dump. Based on the failure characteristics of slope surface, internal stress of slope body and displacement contours during the whole process, we divided the slope instability process in model test into four stages: rainfall infiltration and surface erosion, shallow sliding, deep sliding, and overall instability. Based on the obtained surface deformation data, we calculated the volume change during slope instability process and compared it with the point displacement on slope surface. The results showed that the volume change can not only reflect the slow-ultra acceleration process of slope failure, but also fully reflect the above four stages and reduce the fluctuations caused by random factors. Finally, this paper proposed two stability identification parameters: the volume change rate above the slip surface and the relative velocity of volume change rate. According to the calculation of these two parameters in model test, they can be used for study the deformation and failure mechanism of slope stability.
