Background:Shenque(CV8)acupoint is located on the navel and has been therapeutically used for more than 2000 years in Traditional Chinese Medicine(TCM).However,clinical research on the underlying therapeutic molecular...Background:Shenque(CV8)acupoint is located on the navel and has been therapeutically used for more than 2000 years in Traditional Chinese Medicine(TCM).However,clinical research on the underlying therapeutic molecular mechanisms of the CV8 acupoint lags far behind.This study aimed to study the mechanisms of umbilical acupoint therapy by using stem cells.Methods:The morphological characteristics of CV8 acupoint were detected under a stereomicroscope using hematoxylin and eosin(H&E)staining.Oil Red,Masson,and immunohistochemical staining on multi-layered slices were used to identify the type of cells at the CV8 acupoint.Cell proliferation was measured by a cell counting kit-8(CCK-8)method.Flow cytometry and immunohistochemistry were used for cell identification.Induced differentiation was used to compare the differentiation of cells derived from CV8 acupoint and non-acupoint somatic stem cells into other cell types,such as osteogenic,adipogenic,and neural stem cell-like cells.Results:Morphological observations showed that adipose tissues at the linea alba of the CV8 acupoint in mice had a mass-like distribution.Immunohistochemical staining confirmed the distribution of stem cell antigen-1(Sca-1)positive cells in the multi-layered slices of CV8 acupoint tissues.Cells isolated from adipose tissues at the CV8 acupoint exhibited high expression of Sca-1 and CD44 and low expression of CD31 and CD34,and these cells possessed osteogenic,adipogenic,and neurogenic stem cell-like cell differentiation ability.The cell proliferation(day 4:0.5138±0.0111 vs.0.4107±0.0180,t=8.447,P=0.0011;day 5:0.6890±0.0070 vs.0.5520±0.0118,t=17.310,P<0.0001;day 6:0.7320±0.0090 vs.0.6157±0.0123,t=13.190,P=0.0002;and day 7:0.7550±0.0050 vs.0.6313±0.0051,t=42.560,P<0.0001),adipogenic([9.224±0.345]%vs.[3.933±1.800]%,t=5.000,P=0.0075),and neurogenic stem cell-like cell differentiation(diameter<50 mm:7.2000±1.3040 vs.2.6000±0.5477,t=7.273,P<0.0001;diameter 50–100 mm:2.6000±0.5477 vs.1.0000±0.7071,t=4.000,P=0.0039;and diameter>100 mm:2.6000±0.5477 vs.0.8000±0.8367,t=4.025,P=0.0038)were significantly enhanced in somatic stem cells derived from the CV8 acupoint compared to somatic stem cells from the groin non-acupoint.However,cells possessed significantly weaker osteogenicity([2.697±0.627]%vs.[7.254±0.958]%,t=6.893,P=0.0023)in the CV8 acupoint group.Conclusions:Our study showed that CV8 acupoint was rich with adipose tissues that contained abundant somatic stem cells.The biological examination of somatic stem cells derived from the CV8 acupoint provided novel insights for future research on the mechanisms of umbilical therapy.展开更多
Objective:To observe the clinical efficacy of point application with De Huang(Rhizome Rhei Crude)powder at Shenque(CV 8)plus moxa-salt hot compress on the umbilicus for preventing gastrointestinal adverse reactions af...Objective:To observe the clinical efficacy of point application with De Huang(Rhizome Rhei Crude)powder at Shenque(CV 8)plus moxa-salt hot compress on the umbilicus for preventing gastrointestinal adverse reactions after chemotherapy for non-Hodgkin lymphoma(NHL).Methods:A total of 60 cases with NHL under chemotherapy were divided into two groups by hospitalization order,with 30 cases in each group.The control group was treated with routine nursing and the observation group was additionally given point application with Da Hueng(Rhizome Rhei Crude)powder plus moxa-salt hot compress on the umbilicus,to compare the effect in preventing gastrointestinal adverse reactions after chemotherapy between the two groups.Results:The occurrence rates of constipation,nausea,vomiting and poor appetite on the second day and fifth day after chemotherapy were obviously lower in the observation group than those in the control group,with statistically significant differences between the two groups(all P〈0.05).Conclusion;The point application with De Hueng(Rhizome Rhei Crude)powder at Shenque(CV 8)plus maxa-salt hot compress on the umbilicus can produce more significant efficacy in preventing the gastrointestinal adverse reactions after chemotherapy for NHL than routine nursing.Moreover,it is simple and easy to be used and popularized.展开更多
Objective： To observe the treatment effect of acupoint sticking at Shenque （CV 8） with ginger-preparedBan Xia （Rhizoma Pinelliae） on nausea and vomiting induced by Amifostine for myelodysplastic syndromes （MDS）...Objective： To observe the treatment effect of acupoint sticking at Shenque （CV 8） with ginger-preparedBan Xia （Rhizoma Pinelliae） on nausea and vomiting induced by Amifostine for myelodysplastic syndromes （MDS）. Methods： Totally 124 eligible subjects intervened by Amifostine were randomized into 2 groups by the visiting order,an observation group and a control group,62 in each group. The control group was intervened by conventional treatment, while the observation group was by acupoint sticking at Shenque （CV 8） with ginger-preparedBan Xia （Rhizoma Pinelliae） in addition to the same conventional treatment. The occurrence rate of nausea and vomiting in the two groups were observed. Results： After intervention, the occurrence rate of nausea and vomiting in the observation group was significantly lower than that in the control group （P＆lt;0.01）. Conclusion： Acupoint sticking at Shenque （CV 8） with ginger-prepared Ban Xia （Rhizoma Pinelliae）can produce a content effect on nausea and vomiting induced by Amifostine for MDS.展开更多
Objective： To observe the clinical effect of acupoint sticking of Chinese medicine at Shenque （CV 8） for preventing spleenstomach disharmony caused by venous analgesia pump. Methods： A total of 120 post-surgery pa...Objective： To observe the clinical effect of acupoint sticking of Chinese medicine at Shenque （CV 8） for preventing spleenstomach disharmony caused by venous analgesia pump. Methods： A total of 120 post-surgery patients using venous analgesia pump and also conforming to the inclusion criteria were randomized into 2 groups by the random number table. There were 58 patients in the control group who received conventional post-surgery nursing care, and there were 62 patients in the treatment group who received acupoint sticking at Shenque （CV 8） on the basis of conventional nursing care. After treatment, the incidences of postoperative nausea and vomiting, and abdominal bloating were measured. Results： The total incidence of nausea, vomiting and abdominal bloating in the treatment group was 11.3%, versus 39.7% in the control group, and the difference of the incidence between the two groups showed a statistical significance （P〈0.05）. In comparison of the severity of spleen-stomach dishormony which happened during 72 h after surgery, cases in the treatment group showed lower severity than those in the control group （P〈0.05）. Conclusion： Acupoint sticking at Shenque （CV 8） can effectively prevent spleen-stomach disharmony caused by venous analgesia pump, which will alleviate sufferings of the patients.展开更多
To treat 30 cases of diarrhea of insufficiency of the spleen-yang type by moxibustion with salt at Shenque (CV 8). The total effective rate was 93.3%. Key Words Diarrhea, chronic - Salt-partitioned Moxibustion - Poin...To treat 30 cases of diarrhea of insufficiency of the spleen-yang type by moxibustion with salt at Shenque (CV 8). The total effective rate was 93.3%. Key Words Diarrhea, chronic - Salt-partitioned Moxibustion - Point CV 8 - Acupuncture-moxibustion Translator: WANG Si-you展开更多
Objective： To observe the effect of point sticking therapy on biochemical markers and Rating of Perceived Exertion （RPE） in athletes with exercise-induced fatigue. Methods： A total of 35 football players were rand...Objective： To observe the effect of point sticking therapy on biochemical markers and Rating of Perceived Exertion （RPE） in athletes with exercise-induced fatigue. Methods： A total of 35 football players were randomly allocated into a blank group, a control group and an observation group. The athletes in the blank group received no treatment, those in the control group take Xi Yang Shen （Radix Panacis Quinquefolii） orally and those in the observation group were treated by point sticking therapy. The athletes＇ RPE, hemoglobin （Hb）, red blood cell count （RBC）, blood urea nitrogen （BUN） and blood glucose （BG） were recorded every day for analysis. Results： The RPE value in the blank group tends to increase, while that in the treatment and the blank groups tend to increase first but decrease afterwards, and the corresponding RPE value in the observation group was smaller than that in the control group. After treatment, athletes in the blank group showed a decrease in Hb, RBC and BG, but an increase in BUN in the blank group; while athletes in the observation and the control groups showed increases in Hb, RBC and BG, but a decrease in BUN. Conclusion： Point sticking can alleviate clinical symptoms induced by exercise-induced fatigue.展开更多
Objective:To observe the effects of moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming,and to expl...Objective:To observe the effects of moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming,and to explore the differences between moxibustion at different points in the effects on anti-exercise fatigue.Methods:Forty-eight male SPF rats were randomly divided into a blank group,a model group,a non-meridian and non-acupoint group,a Shenshu(BL 23)group,a Zusanli(ST 36)group,and a Shenque(CV 8)group using random number table method,with eight rats in each group.Except for the blank group,rats in the other groups were subjected to replicating the one-time exhaustive model using the weight-bearing swimming experiment.Except for the model group,the other model rats received mild moxibustion immediately after swimming.Rats in the non-meridian and non-acupoint group received mild moxibustion at bilateral subcostal non-meridian and non-acupoint points,those in the Shenshu(BL 23)group received mild moxibustion at bilateral Shenshu(BL 23),those in the Zusanli(ST 36)group received mild moxibustion at bilateral Zusanli(ST 36),and those in the Shenque(CV 8)group received mild moxibustion at Shenque(CV 8)for 15 min.Four hours after the exhaustive swimming,femoral artery blood was collected to detect blood lactate(BLA),lactate dehydrogenase(LDH),creatine kinase(CK),creatinine(CRE),blood urea nitrogen(BUN),cortisol(C)and testosterone(T)levels,and calculate the T/C ratio.Results:Compared with the blank group,rat's serum levels of BLA,LDH,CK,BUN and C in the model group and the non-meridian and non-acupoint group were increased,and serum levels of CRE and T,and T/C ratios were decreased(P<0.01 or P<0.05);compared with the model group and the non-meridian and non-acupoint group,the serum levels of BLA,LDH,CK,BUN and C in the Shenshu(BL 23)group,Zusanli(ST 36)group and Shenque(CV 8)group were decreased,and the serum CRE and T levels,and the T/C ratios were increased(all P<0.01);compared with the Shenshu(BL 23)group,the serum CK level was decreased in the Shenque(CV 8)group(P<0.01),the serum levels of T and C were decreased in the Zusanli(ST 36)group and Shenque(CV 8)group(P<0.01 or P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.01);compared with the Zusanli(ST 36)group,the serum CK and BUN levels were decreased(P<0.01,P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.05).Conclusion:Moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)shows different anti-fatigue effects by regulating the energy metabolism and endocrine metabolism in rats undergoing one-time exhaustive swimming.Moxibustion at Shenshu(BL 23)is better in promoting energy synthesis.Moxibustion at Shenque(CV 8)is more effective in regulating synthesis and decomposition of the skeletal muscle proteins.展开更多
Male S.D rats weighing 200g were used(as experimental animals).Moxibus-tion at Shenque(RN 8)was performed for 15 minutes each day.The course lasted for weeks.In ratsundergoing oral infusion of taurocholate,index of ga...Male S.D rats weighing 200g were used(as experimental animals).Moxibus-tion at Shenque(RN 8)was performed for 15 minutes each day.The course lasted for weeks.In ratsundergoing oral infusion of taurocholate,index of gastric mucosal injury were 9.0±6.1 and 4.6±2.5 in two and four week groups respectively.Compared with control group(index:16.8±7.6),Pwas less than 0.05 and 0.01.This indicated that moxibustion had protective effect on gastric mucosalinjury.Time-effect relationship also existed.In experiment of analysis about mechanism of moxibustion,we found that moxibustion.1.stim-ulated the secretion of gastric mucus(P【0.001);2.increased the PGE 2 content in gastric mucosa(P【0.05);3.increased the number of β-endorphin-like immunoactive cells in gastric mucosa(P【0.05);4.enhanced the transformation of lymphocytes(P【0.01);5.reinforced the function of an-tibody-producing cells in the spleen.The results suggested that protective effect of moxibustion might result from the increase in mu-cus,the PGE 2 and the endogenous β-endorphin.It might also be related to the reinforcing effect ofmoxibustion on transformation of lymphocytes and antibody-producing function of spleen cells.展开更多
Objective:To investigate the effect of mild moxibustion on transient receptor potential vanilloid type 1(TRPV1)channel expression in primary dysmenorrhea(PD)rats and explore its mechanism in alleviating central pain s...Objective:To investigate the effect of mild moxibustion on transient receptor potential vanilloid type 1(TRPV1)channel expression in primary dysmenorrhea(PD)rats and explore its mechanism in alleviating central pain sensitization.Methods:Thirty-two female non-pregnant Wistar rats were randomized into a blank group,a model group,a mild moxibustion group,and a capsazepine group,with 8 rats in each group.Except for the blank group,the other three groups used estradiol benzoate,ice-water bath,and oxytocin to establish the rat PD model of cold-dampness stagnation pattern.The interventions began on day 1 of modeling,once a day,and lasted 10 d.The mild moxibustion group received mild moxibustion at Shenque(CV8)and Guanyuan(CV4),20 min/time;in the capsazepine group,capsazepine was injected at a dose of 2 mg/(kgbw).The abdominal pain threshold was measured 10-30 min after oxytocin injection on day 11;enzyme-linked immunosorbent assay was used to detect serum prostaglandin F2α(PGF_(2α))level;the expression of TRPV1,cluster of differentiation 11B(CD11B),and proto-oncogene c-Fos in the spinal dorsal horn and hypothalamus was detected by immunofluorescence and Western blotting.Results:Compared to the blank group,the model group showed a decreased pain threshold(P<0.05)and an increased serum PGF_(2α)level with elevated TRPV1,CD11B,and c-Fos protein expression in the spinal dorsal horn and hypothalamus(P<0.05).Compared to the model group,both the mild moxibustion group and capsazepine group showed significantly increased pain thresholds(P<0.05),along with decreased serum PGF_(2α)levels and reduced protein expression levels of TRPV1,CD11B,and c-Fos in the spinal dorsal horn and hypothalamus(P<0.05).Rat pain threshold in the capsazepine group was higher than that in the mild moxibustion group(P<0.05).Serum PGF_(2α)level,the expression levels of CD11B and c-Fos proteins in the spinal dorsal horn,as well as TRPV1,CD11B,and c-Fos proteins in the hypothalamus of the capsazepine group were lower than those in the mild moxibustion group(P<0.05).Conclusion:Mild moxibustion at Shenque(CV8)and Guanyuan(CV4)may alleviate the central pain sensitization in PD rats by down-regulating TRPV1 channel expression in the spinal dorsal horn and hypothalamus,thus playing an analgesic effect.展开更多
OBJECTIVE:Moxibustion has long been thought to promote vital energy or immunity through clinical experience.This study aimed to investigate the clinical effects of indirect moxibustion on antioxidant and immunomodulat...OBJECTIVE:Moxibustion has long been thought to promote vital energy or immunity through clinical experience.This study aimed to investigate the clinical effects of indirect moxibustion on antioxidant and immunomodulation in a normal population.METHODS:Twenty-five healthy volunteers with no objective or subjective disorders were recruited.The participants were treated with indirect moxibustion on acupoints Guanyuan(CV 4) and Shenque(CV 8) three times per week for 4 weeks.The serum levels of reactive oxygen species(ROS) and malondialdehyde(MDA),the total antioxidant capacity(TAC),the activities of catalase and superoxide dismutase(SOD),and the total glutathione content were determined before and after the 12th moxibustion.Lymphocyte subpopulations and 42 cytokines in the peripheral blood were analyzed using flow cytometry and antibody array,respectively.RESULTS:Compared with the initial time point,the serum levels of ROS and MDA were significantly lowered by moxibustion,while TAC was increased(P<0.01 for all).A significant increase was observed in catalase activity(P<0.05),but not in SOD or total glutathione.There were no significant changes in lymphocyte subpopulations or cytokines in the peripheral blood.Fifteen of 25 participants reported at least one symptom in which they felt subjective improvement after moxibustion.CONCLUSION:Indirect moxibustion on acupoints CV 4 and CV 8 improved the antioxidant defense system,which may be a mechanism explaining the clinical effects of moxibustion.展开更多
基金the National Natural Science Foundation Projects(No.81671462)Shanxi Natural Science Foundation Projects(No.201901D211315 and 201901D111184)Science and Technology Develop-ment Program of Xinzhou(No.20180103).
文摘Background:Shenque(CV8)acupoint is located on the navel and has been therapeutically used for more than 2000 years in Traditional Chinese Medicine(TCM).However,clinical research on the underlying therapeutic molecular mechanisms of the CV8 acupoint lags far behind.This study aimed to study the mechanisms of umbilical acupoint therapy by using stem cells.Methods:The morphological characteristics of CV8 acupoint were detected under a stereomicroscope using hematoxylin and eosin(H&E)staining.Oil Red,Masson,and immunohistochemical staining on multi-layered slices were used to identify the type of cells at the CV8 acupoint.Cell proliferation was measured by a cell counting kit-8(CCK-8)method.Flow cytometry and immunohistochemistry were used for cell identification.Induced differentiation was used to compare the differentiation of cells derived from CV8 acupoint and non-acupoint somatic stem cells into other cell types,such as osteogenic,adipogenic,and neural stem cell-like cells.Results:Morphological observations showed that adipose tissues at the linea alba of the CV8 acupoint in mice had a mass-like distribution.Immunohistochemical staining confirmed the distribution of stem cell antigen-1(Sca-1)positive cells in the multi-layered slices of CV8 acupoint tissues.Cells isolated from adipose tissues at the CV8 acupoint exhibited high expression of Sca-1 and CD44 and low expression of CD31 and CD34,and these cells possessed osteogenic,adipogenic,and neurogenic stem cell-like cell differentiation ability.The cell proliferation(day 4:0.5138±0.0111 vs.0.4107±0.0180,t=8.447,P=0.0011;day 5:0.6890±0.0070 vs.0.5520±0.0118,t=17.310,P<0.0001;day 6:0.7320±0.0090 vs.0.6157±0.0123,t=13.190,P=0.0002;and day 7:0.7550±0.0050 vs.0.6313±0.0051,t=42.560,P<0.0001),adipogenic([9.224±0.345]%vs.[3.933±1.800]%,t=5.000,P=0.0075),and neurogenic stem cell-like cell differentiation(diameter<50 mm:7.2000±1.3040 vs.2.6000±0.5477,t=7.273,P<0.0001;diameter 50–100 mm:2.6000±0.5477 vs.1.0000±0.7071,t=4.000,P=0.0039;and diameter>100 mm:2.6000±0.5477 vs.0.8000±0.8367,t=4.025,P=0.0038)were significantly enhanced in somatic stem cells derived from the CV8 acupoint compared to somatic stem cells from the groin non-acupoint.However,cells possessed significantly weaker osteogenicity([2.697±0.627]%vs.[7.254±0.958]%,t=6.893,P=0.0023)in the CV8 acupoint group.Conclusions:Our study showed that CV8 acupoint was rich with adipose tissues that contained abundant somatic stem cells.The biological examination of somatic stem cells derived from the CV8 acupoint provided novel insights for future research on the mechanisms of umbilical therapy.
文摘Objective:To observe the clinical efficacy of point application with De Huang(Rhizome Rhei Crude)powder at Shenque(CV 8)plus moxa-salt hot compress on the umbilicus for preventing gastrointestinal adverse reactions after chemotherapy for non-Hodgkin lymphoma(NHL).Methods:A total of 60 cases with NHL under chemotherapy were divided into two groups by hospitalization order,with 30 cases in each group.The control group was treated with routine nursing and the observation group was additionally given point application with Da Hueng(Rhizome Rhei Crude)powder plus moxa-salt hot compress on the umbilicus,to compare the effect in preventing gastrointestinal adverse reactions after chemotherapy between the two groups.Results:The occurrence rates of constipation,nausea,vomiting and poor appetite on the second day and fifth day after chemotherapy were obviously lower in the observation group than those in the control group,with statistically significant differences between the two groups(all P〈0.05).Conclusion;The point application with De Hueng(Rhizome Rhei Crude)powder at Shenque(CV 8)plus maxa-salt hot compress on the umbilicus can produce more significant efficacy in preventing the gastrointestinal adverse reactions after chemotherapy for NHL than routine nursing.Moreover,it is simple and easy to be used and popularized.
基金supported by Zhejiang Provincial Hospital of Traditional Chinese Medicine
文摘Objective： To observe the treatment effect of acupoint sticking at Shenque （CV 8） with ginger-preparedBan Xia （Rhizoma Pinelliae） on nausea and vomiting induced by Amifostine for myelodysplastic syndromes （MDS）. Methods： Totally 124 eligible subjects intervened by Amifostine were randomized into 2 groups by the visiting order,an observation group and a control group,62 in each group. The control group was intervened by conventional treatment, while the observation group was by acupoint sticking at Shenque （CV 8） with ginger-preparedBan Xia （Rhizoma Pinelliae） in addition to the same conventional treatment. The occurrence rate of nausea and vomiting in the two groups were observed. Results： After intervention, the occurrence rate of nausea and vomiting in the observation group was significantly lower than that in the control group （P＆lt;0.01）. Conclusion： Acupoint sticking at Shenque （CV 8） with ginger-prepared Ban Xia （Rhizoma Pinelliae）can produce a content effect on nausea and vomiting induced by Amifostine for MDS.
文摘Objective： To observe the clinical effect of acupoint sticking of Chinese medicine at Shenque （CV 8） for preventing spleenstomach disharmony caused by venous analgesia pump. Methods： A total of 120 post-surgery patients using venous analgesia pump and also conforming to the inclusion criteria were randomized into 2 groups by the random number table. There were 58 patients in the control group who received conventional post-surgery nursing care, and there were 62 patients in the treatment group who received acupoint sticking at Shenque （CV 8） on the basis of conventional nursing care. After treatment, the incidences of postoperative nausea and vomiting, and abdominal bloating were measured. Results： The total incidence of nausea, vomiting and abdominal bloating in the treatment group was 11.3%, versus 39.7% in the control group, and the difference of the incidence between the two groups showed a statistical significance （P〈0.05）. In comparison of the severity of spleen-stomach dishormony which happened during 72 h after surgery, cases in the treatment group showed lower severity than those in the control group （P〈0.05）. Conclusion： Acupoint sticking at Shenque （CV 8） can effectively prevent spleen-stomach disharmony caused by venous analgesia pump, which will alleviate sufferings of the patients.
文摘To treat 30 cases of diarrhea of insufficiency of the spleen-yang type by moxibustion with salt at Shenque (CV 8). The total effective rate was 93.3%. Key Words Diarrhea, chronic - Salt-partitioned Moxibustion - Point CV 8 - Acupuncture-moxibustion Translator: WANG Si-you
基金supported by Boom Plan Project of Shanghai Municipal Sports Bureau(07TF005)Boom Plan Project of Shanghai Sports Science and Technology(09TF020)+1 种基金Project of Shanghai Science and Technology Committee(10DZ1973800)Shanghai Key Discipline Construction Project(S30304)
文摘Objective： To observe the effect of point sticking therapy on biochemical markers and Rating of Perceived Exertion （RPE） in athletes with exercise-induced fatigue. Methods： A total of 35 football players were randomly allocated into a blank group, a control group and an observation group. The athletes in the blank group received no treatment, those in the control group take Xi Yang Shen （Radix Panacis Quinquefolii） orally and those in the observation group were treated by point sticking therapy. The athletes＇ RPE, hemoglobin （Hb）, red blood cell count （RBC）, blood urea nitrogen （BUN） and blood glucose （BG） were recorded every day for analysis. Results： The RPE value in the blank group tends to increase, while that in the treatment and the blank groups tend to increase first but decrease afterwards, and the corresponding RPE value in the observation group was smaller than that in the control group. After treatment, athletes in the blank group showed a decrease in Hb, RBC and BG, but an increase in BUN in the blank group; while athletes in the observation and the control groups showed increases in Hb, RBC and BG, but a decrease in BUN. Conclusion： Point sticking can alleviate clinical symptoms induced by exercise-induced fatigue.
文摘Objective:To observe the effects of moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming,and to explore the differences between moxibustion at different points in the effects on anti-exercise fatigue.Methods:Forty-eight male SPF rats were randomly divided into a blank group,a model group,a non-meridian and non-acupoint group,a Shenshu(BL 23)group,a Zusanli(ST 36)group,and a Shenque(CV 8)group using random number table method,with eight rats in each group.Except for the blank group,rats in the other groups were subjected to replicating the one-time exhaustive model using the weight-bearing swimming experiment.Except for the model group,the other model rats received mild moxibustion immediately after swimming.Rats in the non-meridian and non-acupoint group received mild moxibustion at bilateral subcostal non-meridian and non-acupoint points,those in the Shenshu(BL 23)group received mild moxibustion at bilateral Shenshu(BL 23),those in the Zusanli(ST 36)group received mild moxibustion at bilateral Zusanli(ST 36),and those in the Shenque(CV 8)group received mild moxibustion at Shenque(CV 8)for 15 min.Four hours after the exhaustive swimming,femoral artery blood was collected to detect blood lactate(BLA),lactate dehydrogenase(LDH),creatine kinase(CK),creatinine(CRE),blood urea nitrogen(BUN),cortisol(C)and testosterone(T)levels,and calculate the T/C ratio.Results:Compared with the blank group,rat's serum levels of BLA,LDH,CK,BUN and C in the model group and the non-meridian and non-acupoint group were increased,and serum levels of CRE and T,and T/C ratios were decreased(P<0.01 or P<0.05);compared with the model group and the non-meridian and non-acupoint group,the serum levels of BLA,LDH,CK,BUN and C in the Shenshu(BL 23)group,Zusanli(ST 36)group and Shenque(CV 8)group were decreased,and the serum CRE and T levels,and the T/C ratios were increased(all P<0.01);compared with the Shenshu(BL 23)group,the serum CK level was decreased in the Shenque(CV 8)group(P<0.01),the serum levels of T and C were decreased in the Zusanli(ST 36)group and Shenque(CV 8)group(P<0.01 or P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.01);compared with the Zusanli(ST 36)group,the serum CK and BUN levels were decreased(P<0.01,P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.05).Conclusion:Moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)shows different anti-fatigue effects by regulating the energy metabolism and endocrine metabolism in rats undergoing one-time exhaustive swimming.Moxibustion at Shenshu(BL 23)is better in promoting energy synthesis.Moxibustion at Shenque(CV 8)is more effective in regulating synthesis and decomposition of the skeletal muscle proteins.
文摘Male S.D rats weighing 200g were used(as experimental animals).Moxibus-tion at Shenque(RN 8)was performed for 15 minutes each day.The course lasted for weeks.In ratsundergoing oral infusion of taurocholate,index of gastric mucosal injury were 9.0±6.1 and 4.6±2.5 in two and four week groups respectively.Compared with control group(index:16.8±7.6),Pwas less than 0.05 and 0.01.This indicated that moxibustion had protective effect on gastric mucosalinjury.Time-effect relationship also existed.In experiment of analysis about mechanism of moxibustion,we found that moxibustion.1.stim-ulated the secretion of gastric mucus(P【0.001);2.increased the PGE 2 content in gastric mucosa(P【0.05);3.increased the number of β-endorphin-like immunoactive cells in gastric mucosa(P【0.05);4.enhanced the transformation of lymphocytes(P【0.01);5.reinforced the function of an-tibody-producing cells in the spleen.The results suggested that protective effect of moxibustion might result from the increase in mu-cus,the PGE 2 and the endogenous β-endorphin.It might also be related to the reinforcing effect ofmoxibustion on transformation of lymphocytes and antibody-producing function of spleen cells.
文摘Objective:To investigate the effect of mild moxibustion on transient receptor potential vanilloid type 1(TRPV1)channel expression in primary dysmenorrhea(PD)rats and explore its mechanism in alleviating central pain sensitization.Methods:Thirty-two female non-pregnant Wistar rats were randomized into a blank group,a model group,a mild moxibustion group,and a capsazepine group,with 8 rats in each group.Except for the blank group,the other three groups used estradiol benzoate,ice-water bath,and oxytocin to establish the rat PD model of cold-dampness stagnation pattern.The interventions began on day 1 of modeling,once a day,and lasted 10 d.The mild moxibustion group received mild moxibustion at Shenque(CV8)and Guanyuan(CV4),20 min/time;in the capsazepine group,capsazepine was injected at a dose of 2 mg/(kgbw).The abdominal pain threshold was measured 10-30 min after oxytocin injection on day 11;enzyme-linked immunosorbent assay was used to detect serum prostaglandin F2α(PGF_(2α))level;the expression of TRPV1,cluster of differentiation 11B(CD11B),and proto-oncogene c-Fos in the spinal dorsal horn and hypothalamus was detected by immunofluorescence and Western blotting.Results:Compared to the blank group,the model group showed a decreased pain threshold(P<0.05)and an increased serum PGF_(2α)level with elevated TRPV1,CD11B,and c-Fos protein expression in the spinal dorsal horn and hypothalamus(P<0.05).Compared to the model group,both the mild moxibustion group and capsazepine group showed significantly increased pain thresholds(P<0.05),along with decreased serum PGF_(2α)levels and reduced protein expression levels of TRPV1,CD11B,and c-Fos in the spinal dorsal horn and hypothalamus(P<0.05).Rat pain threshold in the capsazepine group was higher than that in the mild moxibustion group(P<0.05).Serum PGF_(2α)level,the expression levels of CD11B and c-Fos proteins in the spinal dorsal horn,as well as TRPV1,CD11B,and c-Fos proteins in the hypothalamus of the capsazepine group were lower than those in the mild moxibustion group(P<0.05).Conclusion:Mild moxibustion at Shenque(CV8)and Guanyuan(CV4)may alleviate the central pain sensitization in PD rats by down-regulating TRPV1 channel expression in the spinal dorsal horn and hypothalamus,thus playing an analgesic effect.
文摘OBJECTIVE:Moxibustion has long been thought to promote vital energy or immunity through clinical experience.This study aimed to investigate the clinical effects of indirect moxibustion on antioxidant and immunomodulation in a normal population.METHODS:Twenty-five healthy volunteers with no objective or subjective disorders were recruited.The participants were treated with indirect moxibustion on acupoints Guanyuan(CV 4) and Shenque(CV 8) three times per week for 4 weeks.The serum levels of reactive oxygen species(ROS) and malondialdehyde(MDA),the total antioxidant capacity(TAC),the activities of catalase and superoxide dismutase(SOD),and the total glutathione content were determined before and after the 12th moxibustion.Lymphocyte subpopulations and 42 cytokines in the peripheral blood were analyzed using flow cytometry and antibody array,respectively.RESULTS:Compared with the initial time point,the serum levels of ROS and MDA were significantly lowered by moxibustion,while TAC was increased(P<0.01 for all).A significant increase was observed in catalase activity(P<0.05),but not in SOD or total glutathione.There were no significant changes in lymphocyte subpopulations or cytokines in the peripheral blood.Fifteen of 25 participants reported at least one symptom in which they felt subjective improvement after moxibustion.CONCLUSION:Indirect moxibustion on acupoints CV 4 and CV 8 improved the antioxidant defense system,which may be a mechanism explaining the clinical effects of moxibustion.
