Objective To observe the clinical therapeutic effect on insomnia treated with acupuncture at back-shu points. Methods Sixty-four cases of insomnia were selected and divided randomly into a back-shu points group （grou...Objective To observe the clinical therapeutic effect on insomnia treated with acupuncture at back-shu points. Methods Sixty-four cases of insomnia were selected and divided randomly into a back-shu points group （group A） and a conventional acupoints group （group B） by a computer generated allocation list, 32 cases in each one. In the trial group, acupuncture was applied at xinshu （心俞 15）, Ganshu （刊俞 18）, Pishu （脾俞 BL 20） and Geshu （膈俞 BL 17）. In the control group, acupuncture was applied conventionally to Neiguan （内关 PC 6）, Shenmen （神门 HT 7）, Sanyinjiao （三阴交 SP 6）, Baihui （百会 GV 20） and Sishencong （四神聪 EX-HN 2）. Ten treatments made a session. The efficacy was analyzed statistically after 3 sessions of treatment. Results There were significant differences between the two groups in the relief of sleep disorders after the treatment （P〈0.O2）. The improvement in sleep efficiency was different significantly （P〈0.05）. The difference in sleep difficulty was significant in Chinese medicine （TCM） symptom score （P〈0.05）. Conclusion The clinical efficacy is achieved by either acupuncture at back-shu points or by acupuncture at conventional acupoints. The improvements of sleep efficiency and sleep disorder scores in PSO.I, and sleep difficulty in TCM as well as the long-term efficacy are superior in group A as compared with those in group B.展开更多
Objective To observe the influences of electroacupuncture at the combination of back-shu and front-mu points on insulin sensitivity indicators and somatological parameter indicators in the patients with obesity compli...Objective To observe the influences of electroacupuncture at the combination of back-shu and front-mu points on insulin sensitivity indicators and somatological parameter indicators in the patients with obesity complicated with insulin resistance.Methods A total of 60 patients with obesity and insulin resistance were randomized into a control group(30 cases,4 cases of them dropped out)and a trial group(30 cases,2 cases of them dropped out).In the control group,the intervention with diet and exercise were provided.In the trial group,on the base of the treatment as the control group,electroacupuncture was added at the combination of back-shu points and front-mu points,3 times weekly.The duration of treatment was 12 weeks in two groups.The therapeutic effect and the somatological parameter indicators(body mass[BW],body mass index[BMI],waist circumference[WC],waist-to-hip ratio[WHR])as well as the insulin sensitivity indicators(fasting plasma glucose[FPG],fasting insulin[FIN],insulin sensitivity index[ISI]and insulin resistance index[HOMA-IR])before and after treatment were compared between two groups.Results①The effective rate was 92.86%in the trial group and was 57.69%in the control group.The effective rate of the trial group was higher than the control group,indicating the statistical difference(P<0.01).②BW,BMI,WC and WHR after treatment were all reduced obviously as compared with before treatment in two groups(all P<0.01).After treatment,BMI,WC and WHR in the trial group were all lower than those in the control group(all P<0.05)and the differences in BW,BMI,WC and WHR before and after treatment in the trial group were larger than those in the control group(all P<0.01).③After treatment,FIN and HOMA-IR were all lower significantly than those before treatment in two group and ISI was increased significantly as compared with before treatment(all P<0.01).After treatment,FPG was reduced significantly as compared with before treatment in the trial group(P<0.01)and the difference in FPG was not significant statistically in the control group(P>0.05).After treatment,FPG and HOMA-IR in the trial group were lower than the control group and ISI was higher than the control group(all P<0.05).Besides,the differences in FPG,FIN,ISI and HOMA-IR before and after treatment in the trial group were larger significantly than those in the control group(P<0.01,P<0.05).Conclusion Electroacupuncture at the combination of back-shu and front-mu points in association with the intervention with diet and exercise is effective on obesity complicated with insulin resistance.The effects of this combined therapy are superior as compared with simple intervention with diet and exercise in body mass,body mass index,waist circumference and waist-to-lip ratio as well as fasting blood glucose,fasting insulin,insulin sensitivity index and insulin resistance index in patients.展开更多
BACKGROUND Insomnia is a disease where individuals cannot maintain a steady and stable sleep state or fail to fall asleep.Western medicine mainly uses sedatives and hypnotic drugs to treat insomnia,and long-term use i...BACKGROUND Insomnia is a disease where individuals cannot maintain a steady and stable sleep state or fail to fall asleep.Western medicine mainly uses sedatives and hypnotic drugs to treat insomnia,and long-term use is prone to drug resistance and other adverse reactions.Acupuncture has a good curative effect and unique advantages in the treatment of insomnia.AIM To explore the molecular mechanism of acupuncture at Back-Shu point for the treatment of insomnia.METHODS We first prepared a rat model of insomnia,and then carried out acupuncture for 7 consecutive days.After treatment,the sleep time and general behavior of the rats were determined.The Morris water maze test was used to assess the learning ability and spatial memory ability of the rats.The expression levels of inflammatory cytokines in serum and the hippocampus were detected by ELISA.qRTPCR was used to detect the mRNA expression changes in the ERK/NF-κB signaling pathway.Western blot and immunohistochemistry were carried out to evaluate the protein expression levels of RAF-1,MEK-2,ERK1/2 and NF-κB.RESULTS Acupuncture can prolong sleep duration,and improve mental state,activity,diet volume,learning ability and spatial memory.In addition,acupuncture increased the release of 1L-1β,1L-6 and TNF-αin serum and the hippocampus and inhibited the mRNA and protein expression of the ERK/NF-κB signaling pathway.CONCLUSION These findings suggest that acupuncture at Back-Shu point can inhibit the ERK/NF-κB signaling pathway and treat insomnia by increasing the release of inflammatory cytokines in the hippocampus.展开更多
Objective To find therapeutic effect of acupuncture treating depression. Methods Eighteen cases of depression divided into two groups： one group treated with electroacupuncture （8 cases）, the other with medication ...Objective To find therapeutic effect of acupuncture treating depression. Methods Eighteen cases of depression divided into two groups： one group treated with electroacupuncture （8 cases）, the other with medication （10 cases）. For the group with electroacupuncture, Shenting （神庭 GV 24）, Baihui （百会 GV 20）, Pishu （脾俞 BL 20）, Weishu（胃俞 BL 21）, Dachangshu （大肠俞 BL 25）, Xiaochangshu （小肠俞BL 27） were selected for treatment; and for the group of medication, venlafaxine was given as usual. 30 days later, an open unidirectional qualitative response sequential method was taken to compare the therapeutic effects of two groups. Result The therapeutic effects from two 8 cases proved that the group of electroacupuncture was better than the group of medication. Conclusion For depression, scalp acupuncture accompanied by acupuncture on the Back-shu points has a better effect for depression, and is superior to medication.展开更多
To observe the clinical effect of treatment of acute bronchitis primarily with prickingcupping method on Back-shu points. Methods. The patients of acute bronchitis were randomly divided into 2 groups. In the observati...To observe the clinical effect of treatment of acute bronchitis primarily with prickingcupping method on Back-shu points. Methods. The patients of acute bronchitis were randomly divided into 2 groups. In the observation group, there were 36 cases, who were treated with the integrated traditional Chinese and western medicine, primarily the pricking-cupping method on Back-shu points; while in the control group, there were 29 cases who were given the conventinal treatment of western medicine. All the 2 groups were treated for 7 days as one treating course. Results： The total effective rate of the observation group was 97.2% while that of the control group was 82,8%, so there is a significant difference between them. On the first and third days the clinical manifestations were more satisfactorily improved in the treatment group than in the control group （P 〈 0.01） and on the fifth and seventh days, the comparison showed no significant difference （P〉0.05）. Conclusion： The treatment of acute bronchitis by means of the integrated traditional Chinese and western medicine primarily with pricking-cupping method on Back-shu points is of marked therapeutic effect, simple manipulation, and little untoward effects, thus claiming the unique advantage.展开更多
Objective: To observe the clinical efficacy of acupuncture on back-shu points of five zang in treating chronic fatigue syndrome(CFS).Methods: Sixty patients were randomized into treatment group and control group. In t...Objective: To observe the clinical efficacy of acupuncture on back-shu points of five zang in treating chronic fatigue syndrome(CFS).Methods: Sixty patients were randomized into treatment group and control group. In treatment group,the back-shu points of five zang organs were adopted for acupuncture. Shènshū(肾俞 BL23) was perpendicularly needled 15-30 mm in depth. The rest of the selected points were inserted obliquely 15-30 mm in the direction of the spine with even method of lifting-thrusting and twisting-twirling, 10 s for each acupoint. In control group, the acupoints were approximately 1.5-2 cm away outward from the back-shu points of five zang organs(about on the middle of the two sidelines of the bladder meridian of foottaiyang) and the points from the bladder meridian of foot-taiyang were excluded. Acupoints were needled6-9 mm in depth, and deqi was not required, without any manipulation. The treatments were carried out twice a week for twenty minutes each time, a total of eight treatments were given. The clinical efficacy of acupuncture treatment for CFS was evaluated through the Fatigue Scale-14(FS-14) and the Symptom Check-List 90(SCL-90) after treatment.Results: The total effective rate was 86.67% in the treatment group and 53.33% in the control group(P<0.01). In treatment group, FS-14 total scores, somatic fatigue scores, and mental fatigue scores were significantly reduced than before treatment(all P<0.01). In control group, FS-14 total scores and somatic fatigue scores were significantly reduced(both P<0.01). The improvement of FS-14 total scores, somatic fatigue scores, and mental fatigue scores in the treatment group were better than those in the control group(P<0.01, P<0.05). After treatment, SCL-90 total scores and somatization, compulsion, interpersonal sensitivity, depression, anxiety, hostility, psychoticism, and other factors scores were significantly reduced in the treatment group compared with before treatment(P<0.01). In control group, the SCL-90 total scores and the scores of somatization, compulsion, hostility, and other factors after treatment were lower than those before treatment(P< 0.01). The total scores of SCL-90 and somatization, compulsion, interpersonal sensitivity, depression, anxiety, psychoticism, and other factors in the treatment group were significantly lower than those in the control group(P< 0.01, P< 0.05).Conclusions: Acupuncture on back-shu points of five zang can significantly improve the somatic fatigue and mental fatigue of the patients, and at the same time improve their psychological status. Significant therapeutic effect was found on CFS. Compared with the control group, the acupuncture treatment can significantly improve the patient’s somatic fatigue, mental fatigue and psychological status.展开更多
Objective:To observe the effect of intradermal needle therapy on back-shu points of five zang organs in treating chronic fatigue syndrome(CFS)and provide evidence for the usage of unilateral back-shu point.Methods:Pat...Objective:To observe the effect of intradermal needle therapy on back-shu points of five zang organs in treating chronic fatigue syndrome(CFS)and provide evidence for the usage of unilateral back-shu point.Methods:Patients were randomly divided into bilateral back-shu point group,unilateral back-shu point group and non-acupoint group,remove shedding cases,each group remaining 30 patients.In bilateral back-shu point group,both sides of back-shu points of five zang organs were selected.In unilateral back-shu point group,only one side was selected,left or right alternately.The location where non-acupoint group inserted by intradermal needles were outward from the back-shu points,left or right alternately.Fatigue Scale-14(FS-14)was used to evaluate the fatigue level and the scores’improvement of physical fatigue and mental fatigue revealed the therapeutic effect on body constituents and spirits of CFS patient.Results:After the 8-week intradermal needle therapy,the total effective rate was 83.33%in the bilateral back-shu point group,which was 76.67%in the unilateral back-shu point group and 50.00%in the non-acupoint group.The curative effects of the two groups whose back-shu points of five zang organs were inserted by intradermal needles were better than the non-acupoint group(P<0.05).There was no significant difference in clinical efficacy between the bilateral back-shu point group and the unilateral back-shu point group(P>0.05).There were statistical differences in FS-14 questionnaires including total,physical and mental scores between before and after the treatment in both the two back-shu point groups(P<0.05).Only the scores of total and physical fatigue in decreased in non-acupoint group(P<0.05),which means it couldn’t help to alleviate mental fatigue statistically(P>0.05).Conclusions:(1)The intradermal needle therapy on back-shu points of five zang organs for CFS has a satisfying clinical efficacy in curing tiredness.It can help to reduce both physical and mental fatigue for the patients.Hence,it can regulate both body constituents and spirits simultaneously.(2)Needling the unilateral acupoints can get similar clinical efficacy to the bilateral points.It reveals that we can cut cost by reducing the consumption of the amount of intradermal needles used on one side.(3)Both the groups of back-shu points get better clinical efficacy than the non-acupoint group dramatically.The reason of why the intradermal needle can treat CFS maybe that back-shu points of five zang organs can regulate the functions of five zang organs characteristically.展开更多
Urticaria is a common and frequently encountered disease, which is mainly manifested by extreme pruritus and lumpish eruption of the skin. Acute urticaria with a short disease course can be cured, while the chronic on...Urticaria is a common and frequently encountered disease, which is mainly manifested by extreme pruritus and lumpish eruption of the skin. Acute urticaria with a short disease course can be cured, while the chronic one with repeated attacks is a lingering disorder. In the past few years, 40 cases of urticaria were treated by cupping at the back-shu points of the five zang-organs and Geshu (BL 17). Another 20 cases in the control group were treated with Fang Feng Tong Sheng Wan (防风通圣丸Miraculous Pills of Ledebouriella) and chlorpheniramine maleate. The therapeutic effect in the treatment group was significantly superior to that in the control group. The results are reported as follows.展开更多
3D laser scanning technology is widely used in underground openings for high-precision,rapid,and nondestructive structural evaluations.Segmenting large 3D point cloud datasets,particularly in coal mine roadways with m...3D laser scanning technology is widely used in underground openings for high-precision,rapid,and nondestructive structural evaluations.Segmenting large 3D point cloud datasets,particularly in coal mine roadways with multi-scale targets,remains challenging.This paper proposes an enhanced segmentation method integrating improved PointNet++with a coverage-voted strategy.The coverage-voted strategy reduces data while preserving multi-scale target topology.The segmentation is achieved using an enhanced PointNet++algorithm with a normalization preprocessing head,resulting in a 94%accuracy for common supporting components.Ablation experiments show that the preprocessing head and coverage strategies increase segmentation accuracy by 20%and 2%,respectively,and improve Intersection over Union(IoU)for bearing plate segmentation by 58%and 20%.The accuracy of the current pretraining segmentation model may be affected by variations in surface support components,but it can be readily enhanced through re-optimization with additional labeled point cloud data.This proposed method,combined with a previously developed machine learning model that links rock bolt load and the deformation field of its bearing plate,provides a robust technique for simultaneously measuring the load of multiple rock bolts in a single laser scan.展开更多
针对自动驾驶场景下,近处干扰点云误检率高、远处稀疏点云漏检率高的问题,提出了一种基于改进PointPillars的自动驾驶障碍物点云检测算法.首先,通过聚合模块和共享多层感知机(shared multi-layer perceptron,MLP)对柱体内点云进行特征编...针对自动驾驶场景下,近处干扰点云误检率高、远处稀疏点云漏检率高的问题,提出了一种基于改进PointPillars的自动驾驶障碍物点云检测算法.首先,通过聚合模块和共享多层感知机(shared multi-layer perceptron,MLP)对柱体内点云进行特征编码,采用最大池化与平均池化叠加的方法将点云的显著特征与细节特征映射为柱体特征;其次,针对算法对伪图特征关注与利用不充分的问题,引入坐标注意力(coordinate attention,CA)机制和残差连接的伪图特征提取模块(attention and residual second block,ARSB),将深层与浅层特征图进行融合,优化算法梯度,增强算法对有效目标的关注度.试验结果表明:改进算法对全局点云检测精度较高,平均精度优于PointPillars、稀疏到稠密3D目标检测器(STD)等点云目标检测算法,在汽车类别上的检测精度优势明显,检测速度较快,符合实时性要求.展开更多
The strong connection between braids and knots provides valuable insights into studying the topological state and phase classification of various physical systems.The phenomenon of non-Hermitian(NH)two-and three-band ...The strong connection between braids and knots provides valuable insights into studying the topological state and phase classification of various physical systems.The phenomenon of non-Hermitian(NH)two-and three-band braiding has received widespread attention.However,a systematic exploration and visualization of non-Abelian braiding and the associated knot transformations in four-band systems remains unexplored.Here,we propose a theoretical model of NH four-band braiding,provide its phase diagram,and establish its trivial,Abelian,and non-Abelian braiding rules.Additionally,we report on special knots,such as the Hopf and Solomon links in braided knots,and reveal that their transformations are accompanied by and mediated through exceptional points.Our work provides a detailed case for studying NH multiband braiding and knot structures in four-band systems,which could offer insights for topological photonics and analog information processing applications.展开更多
Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewa...Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewable,and clinical demand is increasingly difficult to meet,leading to a proliferation of counterfeit products.During prolonged geological burial,static pressure from the surrounding strata severely compromises the microstructural integrity of osteons in Os Draconis,but Os Draconis still largely retains the structural features of mammalian bone.Methods:Using verified authentic Os Draconis samples over 10,000 years old as a baseline,this study summarizes the ultrastructural characteristics of genuine Os Draconis.Employing electron probe microanalysis and optical polarized light microscopy,we examined 28 batches of authentic Os Draconis and 31 batches of counterfeits to identify their ultrastructural differences.Key points for ultrastructural identification of Os Draconis were compiled,and a new identification approach was proposed based on these differences.Results:Authentic Os Draconis exhibited distinct ultrastructural markers:irregularly shaped osteons with traversing fissures,deformed/displaced Haversian canals,and secondary mineral infill(predominantly calcium carbonate).Counterfeits showed regular osteon arrangements,absent traversal fissures,and homogeneous hydroxyapatite composition.Lab-simulated samples lacked structural degradation features.EPMA confirmed calcium carbonate infill in fossilized Haversian canals,while elemental profiles differentiated lacunae types(void vs.mineral-packed).Conclusion:The study established ultrastructural criteria for authentic Os Draconis identification:osteon deformation,geological fissures penetrating bone units,and heterogenous mineral deposition.These features,unattainable in counterfeits or modern processed bones,provide a cost-effective,accurate identification method.This approach bridges gaps in TCM material standardization and supports quality control for clinical applications.展开更多
Recently,large-scale deep learning models have been increasingly adopted for point cloud classification.However,thesemethods typically require collecting extensive datasets frommultiple clients,which may lead to priva...Recently,large-scale deep learning models have been increasingly adopted for point cloud classification.However,thesemethods typically require collecting extensive datasets frommultiple clients,which may lead to privacy leaks.Federated learning provides an effective solution to data leakage by eliminating the need for data transmission,relying instead on the exchange of model parameters.However,the uneven distribution of client data can still affect the model’s ability to generalize effectively.To address these challenges,we propose a new framework for point cloud classification called Federated Dynamic Aggregation Selection Strategy-based Multi-Receptive Field Fusion Classification Framework(FDASS-MRFCF).Specifically,we tackle these challenges with two key innovations:(1)During the client local training phase,we propose a Multi-Receptive Field Fusion Classification Model(MRFCM),which captures local and global structures in point cloud data through dynamic convolution and multi-scale feature fusion,enhancing the robustness of point cloud classification.(2)In the server aggregation phase,we introduce a Federated Dynamic Aggregation Selection Strategy(FDASS),which employs a hybrid strategy to average client model parameters,skip aggregation,or reallocate local models to different clients,thereby balancing global consistency and local diversity.We evaluate our framework using the ModelNet40 and ShapeNetPart benchmarks,demonstrating its effectiveness.The proposed method is expected to significantly advance the field of point cloud classification in a secure environment.展开更多
Objective To investigate whether Tuina alleviates fibrotic symptoms in myofascial trigger points(MTrPs)by regulating transforming growth factor(TGF)-β1/Smad3 signaling pathway,thereby deactivating these points.Method...Objective To investigate whether Tuina alleviates fibrotic symptoms in myofascial trigger points(MTrPs)by regulating transforming growth factor(TGF)-β1/Smad3 signaling pathway,thereby deactivating these points.Methods This study comprised two experimental phases.In phase 1,27 specific pathogenfree(SPF)grade female Sprague-Dawley(SD)rats were randomized into three groups:control 1,model 1,and Tuina 1 groups.Model 1 and Tuina 1 groups underwent an 8-week MTrPs modeling protocol involving blunt impact and eccentric exercise.After successful modeling,rats in Tuina 1 group received manual pressing on nodules or cord-like taut bands on the medial aspect of the left hindlimb.Pain sensitivity and tissue stiffness were evaluated via pressure pain threshold(PPT)and soft tissue tension(STT).Muscle histopathology and fibrosis were observed using hematoxylin and eosin(HE)and Masson staining.Inflammatory factors in muscle were measured by enzyme-linked immunosorbent assay(ELISA),while immunofluorescence(IF)and Western blot(WB)were used to detect the expression levels ofα-smooth muscle actin(α-SMA),collagenⅢ,and TGF-β1.In phase 2,45 SPF female SD rats were randomized into five groups:control 2,model 2,Tuina 2,TGF-β1 inhibitor(TI),and Tuina+TGF-β1 agonist(Tuina+TA)groups.All groups except control 2 underwent standardized MTrPs modeling.Rats in Tuina 2 group received consistent pressing manipulation.TI group received intraperitoneal injections of oxymatrine,while Tuina+TA group received intraperitoneal injections of SRI-011381 hydrochloride followed by the same pressing protocol as Tuina 2 group.WB was used to detect the expression of collagen I,collagen III,TGF-β1,and phosphorylated-Smad3(p-Smad3)/Smad3.Results In phase 1,Tuina significantly improved PPT and STT in MTrPs of rats(P<0.01),reversed pathological damages including disorganized muscle fiber arrangement,abnormal myocyte morphology,and exacerbated fibrosis.In addition,in MTrPs of rats in model 1 group,expression levels of nuclear factor kappa-light-chain-enhancer of activated B cells(NF-κB),interleukin(IL)-1β,IL-6,tumor necrosis factor(TNF)-α,and fibrosis markers(α-SMA,collagen I,and collagen III)were upregulated,and all exhibited a significant downward trend after Tuina intervention(P<0.05 or P<0.01).This indicates that the therapeutic effects of Tuina are directly associated with reduced local inflammation and fibrosis in MTrPs.In phase 2,compared with model 2 group,rats in TI and Tuina 2 groups had decreased expression levels of TGF-β1 and p-Smad3/Smad3 in MTrPs,alongside reduced levels of inflammatory factors(IL-1β,IL-6,NF-κB,and TNF-α)and fibrosis markers(α-SMA,collagen I,and collagen III)(P<0.05 or P<0.01).When co-administered with TGF-β1 agonist,the therapeutic effects of Tuina were significantly attenuated,with rebounded TGF-β1 expression and p-Smad3/Smad3 in local MTrPs,and fibrosis and inflammatory responses were re-exacerbated(P<0.05 or P<0.01).Conclusion Tuina can effectively reduce inflammatory responses and fibrosis in MTrPs tissue,and its mechanism is closely related to the inhibition of the TGF-β1/Smad3 signaling pathway,which plays a critical role in Tuina-mediated regulation of MTrPs fibrosis.展开更多
For India to achieve elimination by 2030,the challenges posed by Plasmodium(P.)vivax cannot be overlooked owing to its burden and unique biology.In 2023,in India,about 224000 malaria cases were reported,and a signific...For India to achieve elimination by 2030,the challenges posed by Plasmodium(P.)vivax cannot be overlooked owing to its burden and unique biology.In 2023,in India,about 224000 malaria cases were reported,and a significant proportion(40%)were P.vivax cases.In P.vivax infection,the persistence of dormant liver stage of parasite,i.e.,hypnozoites,leading to relapses weeks or months later poses challenge in its elimination.展开更多
AIM:To construct an intelligent segmentation scheme for precise localization of central serous chorioretinopathy(CSC)leakage points,thereby enabling ophthalmologists to deliver accurate laser treatment without navigat...AIM:To construct an intelligent segmentation scheme for precise localization of central serous chorioretinopathy(CSC)leakage points,thereby enabling ophthalmologists to deliver accurate laser treatment without navigational laser equipment.METHODS:A dataset with dual labels(point-level and pixel-level)was first established based on fundus fluorescein angiography(FFA)images of CSC and subsequently divided into training(102 images),validation(40 images),and test(40 images)datasets.An intelligent segmentation method was then developed,based on the You Only Look Once version 8 Pose Estimation(YOLOv8-Pose)model and segment anything model(SAM),to segment CSC leakage points.Next,the YOLOv8-Pose model was trained for 200 epochs,and the best-performing model was selected to form the optimal combination with SAM.Additionally,the classic five types of U-Net series models[i.e.,U-Net,recurrent residual U-Net(R2U-Net),attention U-Net(AttU-Net),recurrent residual attention U-Net(R2AttUNet),and nested U-Net(UNet^(++))]were initialized with three random seeds and trained for 200 epochs,resulting in a total of 15 baseline models for comparison.Finally,based on the metrics including Dice similarity coefficient(DICE),intersection over union(IoU),precision,recall,precisionrecall(PR)curve,and receiver operating characteristic(ROC)curve,the proposed method was compared with baseline models through quantitative and qualitative experiments for leakage point segmentation,thereby demonstrating its effectiveness.RESULTS:With the increase of training epochs,the mAP50-95,Recall,and precision of the YOLOv8-Pose model showed a significant increase and tended to stabilize,and it achieved a preliminary localization success rate of 90%(i.e.,36 images)for CSC leakage points in 40 test images.Using manually expert-annotated pixel-level labels as the ground truth,the proposed method achieved outcomes with a DICE of 57.13%,an IoU of 45.31%,a precision of 45.91%,a recall of 93.57%,an area under the PR curve(AUC-PR)of 0.78 and an area under the ROC curve(AUC-ROC)of 0.97,which enables more accurate segmentation of CSC leakage points.CONCLUSION:By combining the precise localization capability of the YOLOv8-Pose model with the robust and flexible segmentation ability of SAM,the proposed method not only demonstrates the effectiveness of the YOLOv8-Pose model in detecting keypoint coordinates of CSC leakage points from the perspective of application innovation but also establishes a novel approach for accurate segmentation of CSC leakage points through the“detect-then-segment”strategy,thereby providing a potential auxiliary means for the automatic and precise realtime localization of leakage points during traditional laser photocoagulation for CSC.展开更多
Highlights By conjugating the same anti-N monoclonal antibody(mAb4-mAb1)with colloidal gold or fluorescent microspheres,this study developed two rapid point-of-care antigen immunochromatographic strips for the detecti...Highlights By conjugating the same anti-N monoclonal antibody(mAb4-mAb1)with colloidal gold or fluorescent microspheres,this study developed two rapid point-of-care antigen immunochromatographic strips for the detection of porcine deltacoronavirus.The fluorescent microsphere-based lateral flow test strip demonstrated a sensitivity of 10^(1.7)TCID_(50)/0.1 mL,which is fourfold higher than that of the colloidal gold-based assay.Porcine deltacoronavirus(PDCoV)is a recently identified enteric coronavirus that causes an acute infectious disease in piglets,leading to diarrhea,vomiting,dehydration,and mortality(Hu et al.2015).展开更多
基金Supported by National Natural Science Foundation:No.81173335
文摘Objective To observe the clinical therapeutic effect on insomnia treated with acupuncture at back-shu points. Methods Sixty-four cases of insomnia were selected and divided randomly into a back-shu points group （group A） and a conventional acupoints group （group B） by a computer generated allocation list, 32 cases in each one. In the trial group, acupuncture was applied at xinshu （心俞 15）, Ganshu （刊俞 18）, Pishu （脾俞 BL 20） and Geshu （膈俞 BL 17）. In the control group, acupuncture was applied conventionally to Neiguan （内关 PC 6）, Shenmen （神门 HT 7）, Sanyinjiao （三阴交 SP 6）, Baihui （百会 GV 20） and Sishencong （四神聪 EX-HN 2）. Ten treatments made a session. The efficacy was analyzed statistically after 3 sessions of treatment. Results There were significant differences between the two groups in the relief of sleep disorders after the treatment （P〈0.O2）. The improvement in sleep efficiency was different significantly （P〈0.05）. The difference in sleep difficulty was significant in Chinese medicine （TCM） symptom score （P〈0.05）. Conclusion The clinical efficacy is achieved by either acupuncture at back-shu points or by acupuncture at conventional acupoints. The improvements of sleep efficiency and sleep disorder scores in PSO.I, and sleep difficulty in TCM as well as the long-term efficacy are superior in group A as compared with those in group B.
基金Supported by Clinical Medical Technology Innovation Guidance Project of Hunan Province:2017SK50114。
文摘Objective To observe the influences of electroacupuncture at the combination of back-shu and front-mu points on insulin sensitivity indicators and somatological parameter indicators in the patients with obesity complicated with insulin resistance.Methods A total of 60 patients with obesity and insulin resistance were randomized into a control group(30 cases,4 cases of them dropped out)and a trial group(30 cases,2 cases of them dropped out).In the control group,the intervention with diet and exercise were provided.In the trial group,on the base of the treatment as the control group,electroacupuncture was added at the combination of back-shu points and front-mu points,3 times weekly.The duration of treatment was 12 weeks in two groups.The therapeutic effect and the somatological parameter indicators(body mass[BW],body mass index[BMI],waist circumference[WC],waist-to-hip ratio[WHR])as well as the insulin sensitivity indicators(fasting plasma glucose[FPG],fasting insulin[FIN],insulin sensitivity index[ISI]and insulin resistance index[HOMA-IR])before and after treatment were compared between two groups.Results①The effective rate was 92.86%in the trial group and was 57.69%in the control group.The effective rate of the trial group was higher than the control group,indicating the statistical difference(P<0.01).②BW,BMI,WC and WHR after treatment were all reduced obviously as compared with before treatment in two groups(all P<0.01).After treatment,BMI,WC and WHR in the trial group were all lower than those in the control group(all P<0.05)and the differences in BW,BMI,WC and WHR before and after treatment in the trial group were larger than those in the control group(all P<0.01).③After treatment,FIN and HOMA-IR were all lower significantly than those before treatment in two group and ISI was increased significantly as compared with before treatment(all P<0.01).After treatment,FPG was reduced significantly as compared with before treatment in the trial group(P<0.01)and the difference in FPG was not significant statistically in the control group(P>0.05).After treatment,FPG and HOMA-IR in the trial group were lower than the control group and ISI was higher than the control group(all P<0.05).Besides,the differences in FPG,FIN,ISI and HOMA-IR before and after treatment in the trial group were larger significantly than those in the control group(P<0.01,P<0.05).Conclusion Electroacupuncture at the combination of back-shu and front-mu points in association with the intervention with diet and exercise is effective on obesity complicated with insulin resistance.The effects of this combined therapy are superior as compared with simple intervention with diet and exercise in body mass,body mass index,waist circumference and waist-to-lip ratio as well as fasting blood glucose,fasting insulin,insulin sensitivity index and insulin resistance index in patients.
文摘BACKGROUND Insomnia is a disease where individuals cannot maintain a steady and stable sleep state or fail to fall asleep.Western medicine mainly uses sedatives and hypnotic drugs to treat insomnia,and long-term use is prone to drug resistance and other adverse reactions.Acupuncture has a good curative effect and unique advantages in the treatment of insomnia.AIM To explore the molecular mechanism of acupuncture at Back-Shu point for the treatment of insomnia.METHODS We first prepared a rat model of insomnia,and then carried out acupuncture for 7 consecutive days.After treatment,the sleep time and general behavior of the rats were determined.The Morris water maze test was used to assess the learning ability and spatial memory ability of the rats.The expression levels of inflammatory cytokines in serum and the hippocampus were detected by ELISA.qRTPCR was used to detect the mRNA expression changes in the ERK/NF-κB signaling pathway.Western blot and immunohistochemistry were carried out to evaluate the protein expression levels of RAF-1,MEK-2,ERK1/2 and NF-κB.RESULTS Acupuncture can prolong sleep duration,and improve mental state,activity,diet volume,learning ability and spatial memory.In addition,acupuncture increased the release of 1L-1β,1L-6 and TNF-αin serum and the hippocampus and inhibited the mRNA and protein expression of the ERK/NF-κB signaling pathway.CONCLUSION These findings suggest that acupuncture at Back-Shu point can inhibit the ERK/NF-κB signaling pathway and treat insomnia by increasing the release of inflammatory cytokines in the hippocampus.
文摘Objective To find therapeutic effect of acupuncture treating depression. Methods Eighteen cases of depression divided into two groups： one group treated with electroacupuncture （8 cases）, the other with medication （10 cases）. For the group with electroacupuncture, Shenting （神庭 GV 24）, Baihui （百会 GV 20）, Pishu （脾俞 BL 20）, Weishu（胃俞 BL 21）, Dachangshu （大肠俞 BL 25）, Xiaochangshu （小肠俞BL 27） were selected for treatment; and for the group of medication, venlafaxine was given as usual. 30 days later, an open unidirectional qualitative response sequential method was taken to compare the therapeutic effects of two groups. Result The therapeutic effects from two 8 cases proved that the group of electroacupuncture was better than the group of medication. Conclusion For depression, scalp acupuncture accompanied by acupuncture on the Back-shu points has a better effect for depression, and is superior to medication.
文摘To observe the clinical effect of treatment of acute bronchitis primarily with prickingcupping method on Back-shu points. Methods. The patients of acute bronchitis were randomly divided into 2 groups. In the observation group, there were 36 cases, who were treated with the integrated traditional Chinese and western medicine, primarily the pricking-cupping method on Back-shu points; while in the control group, there were 29 cases who were given the conventinal treatment of western medicine. All the 2 groups were treated for 7 days as one treating course. Results： The total effective rate of the observation group was 97.2% while that of the control group was 82,8%, so there is a significant difference between them. On the first and third days the clinical manifestations were more satisfactorily improved in the treatment group than in the control group （P 〈 0.01） and on the fifth and seventh days, the comparison showed no significant difference （P〉0.05）. Conclusion： The treatment of acute bronchitis by means of the integrated traditional Chinese and western medicine primarily with pricking-cupping method on Back-shu points is of marked therapeutic effect, simple manipulation, and little untoward effects, thus claiming the unique advantage.
基金Supported by the subject of clinical research on treating chronic fatigue syndrome by acupuncture on the reaction point of back-shu points in Dongzhimen Hospital Beijing University of Chinese Medicine:2016-DZM111-JC006~~
文摘Objective: To observe the clinical efficacy of acupuncture on back-shu points of five zang in treating chronic fatigue syndrome(CFS).Methods: Sixty patients were randomized into treatment group and control group. In treatment group,the back-shu points of five zang organs were adopted for acupuncture. Shènshū(肾俞 BL23) was perpendicularly needled 15-30 mm in depth. The rest of the selected points were inserted obliquely 15-30 mm in the direction of the spine with even method of lifting-thrusting and twisting-twirling, 10 s for each acupoint. In control group, the acupoints were approximately 1.5-2 cm away outward from the back-shu points of five zang organs(about on the middle of the two sidelines of the bladder meridian of foottaiyang) and the points from the bladder meridian of foot-taiyang were excluded. Acupoints were needled6-9 mm in depth, and deqi was not required, without any manipulation. The treatments were carried out twice a week for twenty minutes each time, a total of eight treatments were given. The clinical efficacy of acupuncture treatment for CFS was evaluated through the Fatigue Scale-14(FS-14) and the Symptom Check-List 90(SCL-90) after treatment.Results: The total effective rate was 86.67% in the treatment group and 53.33% in the control group(P<0.01). In treatment group, FS-14 total scores, somatic fatigue scores, and mental fatigue scores were significantly reduced than before treatment(all P<0.01). In control group, FS-14 total scores and somatic fatigue scores were significantly reduced(both P<0.01). The improvement of FS-14 total scores, somatic fatigue scores, and mental fatigue scores in the treatment group were better than those in the control group(P<0.01, P<0.05). After treatment, SCL-90 total scores and somatization, compulsion, interpersonal sensitivity, depression, anxiety, hostility, psychoticism, and other factors scores were significantly reduced in the treatment group compared with before treatment(P<0.01). In control group, the SCL-90 total scores and the scores of somatization, compulsion, hostility, and other factors after treatment were lower than those before treatment(P< 0.01). The total scores of SCL-90 and somatization, compulsion, interpersonal sensitivity, depression, anxiety, psychoticism, and other factors in the treatment group were significantly lower than those in the control group(P< 0.01, P< 0.05).Conclusions: Acupuncture on back-shu points of five zang can significantly improve the somatic fatigue and mental fatigue of the patients, and at the same time improve their psychological status. Significant therapeutic effect was found on CFS. Compared with the control group, the acupuncture treatment can significantly improve the patient’s somatic fatigue, mental fatigue and psychological status.
基金Xin'ao Award Funded Project of Beijing University of Chinese Medicine(No.2018-XAJLJJ-015)。
文摘Objective:To observe the effect of intradermal needle therapy on back-shu points of five zang organs in treating chronic fatigue syndrome(CFS)and provide evidence for the usage of unilateral back-shu point.Methods:Patients were randomly divided into bilateral back-shu point group,unilateral back-shu point group and non-acupoint group,remove shedding cases,each group remaining 30 patients.In bilateral back-shu point group,both sides of back-shu points of five zang organs were selected.In unilateral back-shu point group,only one side was selected,left or right alternately.The location where non-acupoint group inserted by intradermal needles were outward from the back-shu points,left or right alternately.Fatigue Scale-14(FS-14)was used to evaluate the fatigue level and the scores’improvement of physical fatigue and mental fatigue revealed the therapeutic effect on body constituents and spirits of CFS patient.Results:After the 8-week intradermal needle therapy,the total effective rate was 83.33%in the bilateral back-shu point group,which was 76.67%in the unilateral back-shu point group and 50.00%in the non-acupoint group.The curative effects of the two groups whose back-shu points of five zang organs were inserted by intradermal needles were better than the non-acupoint group(P<0.05).There was no significant difference in clinical efficacy between the bilateral back-shu point group and the unilateral back-shu point group(P>0.05).There were statistical differences in FS-14 questionnaires including total,physical and mental scores between before and after the treatment in both the two back-shu point groups(P<0.05).Only the scores of total and physical fatigue in decreased in non-acupoint group(P<0.05),which means it couldn’t help to alleviate mental fatigue statistically(P>0.05).Conclusions:(1)The intradermal needle therapy on back-shu points of five zang organs for CFS has a satisfying clinical efficacy in curing tiredness.It can help to reduce both physical and mental fatigue for the patients.Hence,it can regulate both body constituents and spirits simultaneously.(2)Needling the unilateral acupoints can get similar clinical efficacy to the bilateral points.It reveals that we can cut cost by reducing the consumption of the amount of intradermal needles used on one side.(3)Both the groups of back-shu points get better clinical efficacy than the non-acupoint group dramatically.The reason of why the intradermal needle can treat CFS maybe that back-shu points of five zang organs can regulate the functions of five zang organs characteristically.
文摘Urticaria is a common and frequently encountered disease, which is mainly manifested by extreme pruritus and lumpish eruption of the skin. Acute urticaria with a short disease course can be cured, while the chronic one with repeated attacks is a lingering disorder. In the past few years, 40 cases of urticaria were treated by cupping at the back-shu points of the five zang-organs and Geshu (BL 17). Another 20 cases in the control group were treated with Fang Feng Tong Sheng Wan (防风通圣丸Miraculous Pills of Ledebouriella) and chlorpheniramine maleate. The therapeutic effect in the treatment group was significantly superior to that in the control group. The results are reported as follows.
基金supported by the National Natural Science Foundation of China(Grant Nos.52304139,52325403)the CCTEG Coal Mining Research Institute funding(Grant No.KCYJY-2024-MS-10).
文摘3D laser scanning technology is widely used in underground openings for high-precision,rapid,and nondestructive structural evaluations.Segmenting large 3D point cloud datasets,particularly in coal mine roadways with multi-scale targets,remains challenging.This paper proposes an enhanced segmentation method integrating improved PointNet++with a coverage-voted strategy.The coverage-voted strategy reduces data while preserving multi-scale target topology.The segmentation is achieved using an enhanced PointNet++algorithm with a normalization preprocessing head,resulting in a 94%accuracy for common supporting components.Ablation experiments show that the preprocessing head and coverage strategies increase segmentation accuracy by 20%and 2%,respectively,and improve Intersection over Union(IoU)for bearing plate segmentation by 58%and 20%.The accuracy of the current pretraining segmentation model may be affected by variations in surface support components,but it can be readily enhanced through re-optimization with additional labeled point cloud data.This proposed method,combined with a previously developed machine learning model that links rock bolt load and the deformation field of its bearing plate,provides a robust technique for simultaneously measuring the load of multiple rock bolts in a single laser scan.
文摘针对自动驾驶场景下,近处干扰点云误检率高、远处稀疏点云漏检率高的问题,提出了一种基于改进PointPillars的自动驾驶障碍物点云检测算法.首先,通过聚合模块和共享多层感知机(shared multi-layer perceptron,MLP)对柱体内点云进行特征编码,采用最大池化与平均池化叠加的方法将点云的显著特征与细节特征映射为柱体特征;其次,针对算法对伪图特征关注与利用不充分的问题,引入坐标注意力(coordinate attention,CA)机制和残差连接的伪图特征提取模块(attention and residual second block,ARSB),将深层与浅层特征图进行融合,优化算法梯度,增强算法对有效目标的关注度.试验结果表明:改进算法对全局点云检测精度较高,平均精度优于PointPillars、稀疏到稠密3D目标检测器(STD)等点云目标检测算法,在汽车类别上的检测精度优势明显,检测速度较快,符合实时性要求.
基金supported by the National Natural Science Foundation of China(Grant Nos.62575099,62075059,61405058)Guangdong Basic and Applied Basic Research Foundation(Grant No.2024A1515011353)+2 种基金Open Project of the State Key Laboratory of Advanced Optical Communication Systems and Networks of China(Grant No.2024GZKF20)the Natural Science Foundation of Hunan Province(Grant Nos.2020JJ4161 and 2017JJ2048)Scientific Research Foundation of Hunan Provincial Education Department(Grant No.21A0013)。
文摘The strong connection between braids and knots provides valuable insights into studying the topological state and phase classification of various physical systems.The phenomenon of non-Hermitian(NH)two-and three-band braiding has received widespread attention.However,a systematic exploration and visualization of non-Abelian braiding and the associated knot transformations in four-band systems remains unexplored.Here,we propose a theoretical model of NH four-band braiding,provide its phase diagram,and establish its trivial,Abelian,and non-Abelian braiding rules.Additionally,we report on special knots,such as the Hopf and Solomon links in braided knots,and reveal that their transformations are accompanied by and mediated through exceptional points.Our work provides a detailed case for studying NH multiband braiding and knot structures in four-band systems,which could offer insights for topological photonics and analog information processing applications.
基金supported by the Scientific and Technological Innovation Project of the China Academy of Chinese Medical Sciences(CI2021A04013)the National Natural Science Foundation of China(82204610)+1 种基金the Qihang Talent Program(L2022046)the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ15-YQ-041 and L2021029).
文摘Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewable,and clinical demand is increasingly difficult to meet,leading to a proliferation of counterfeit products.During prolonged geological burial,static pressure from the surrounding strata severely compromises the microstructural integrity of osteons in Os Draconis,but Os Draconis still largely retains the structural features of mammalian bone.Methods:Using verified authentic Os Draconis samples over 10,000 years old as a baseline,this study summarizes the ultrastructural characteristics of genuine Os Draconis.Employing electron probe microanalysis and optical polarized light microscopy,we examined 28 batches of authentic Os Draconis and 31 batches of counterfeits to identify their ultrastructural differences.Key points for ultrastructural identification of Os Draconis were compiled,and a new identification approach was proposed based on these differences.Results:Authentic Os Draconis exhibited distinct ultrastructural markers:irregularly shaped osteons with traversing fissures,deformed/displaced Haversian canals,and secondary mineral infill(predominantly calcium carbonate).Counterfeits showed regular osteon arrangements,absent traversal fissures,and homogeneous hydroxyapatite composition.Lab-simulated samples lacked structural degradation features.EPMA confirmed calcium carbonate infill in fossilized Haversian canals,while elemental profiles differentiated lacunae types(void vs.mineral-packed).Conclusion:The study established ultrastructural criteria for authentic Os Draconis identification:osteon deformation,geological fissures penetrating bone units,and heterogenous mineral deposition.These features,unattainable in counterfeits or modern processed bones,provide a cost-effective,accurate identification method.This approach bridges gaps in TCM material standardization and supports quality control for clinical applications.
基金supported in part by the National Key Research and Development Program of Chinaunder(Grant 2021YFB3101100)in part by the National Natural Science Foundation of Chinaunder(Grant 42461057),(Grant 62272123),and(Grant 42371470)+1 种基金in part by the Fundamental Research Program of Shanxi Province under(Grant 202303021212164)in part by the Postgraduate Education Innovation Program of Shanxi Province under(Grant 2024KY474).
文摘Recently,large-scale deep learning models have been increasingly adopted for point cloud classification.However,thesemethods typically require collecting extensive datasets frommultiple clients,which may lead to privacy leaks.Federated learning provides an effective solution to data leakage by eliminating the need for data transmission,relying instead on the exchange of model parameters.However,the uneven distribution of client data can still affect the model’s ability to generalize effectively.To address these challenges,we propose a new framework for point cloud classification called Federated Dynamic Aggregation Selection Strategy-based Multi-Receptive Field Fusion Classification Framework(FDASS-MRFCF).Specifically,we tackle these challenges with two key innovations:(1)During the client local training phase,we propose a Multi-Receptive Field Fusion Classification Model(MRFCM),which captures local and global structures in point cloud data through dynamic convolution and multi-scale feature fusion,enhancing the robustness of point cloud classification.(2)In the server aggregation phase,we introduce a Federated Dynamic Aggregation Selection Strategy(FDASS),which employs a hybrid strategy to average client model parameters,skip aggregation,or reallocate local models to different clients,thereby balancing global consistency and local diversity.We evaluate our framework using the ModelNet40 and ShapeNetPart benchmarks,demonstrating its effectiveness.The proposed method is expected to significantly advance the field of point cloud classification in a secure environment.
基金Natural Science Foundation of China(82274676 and 82374613)Program of Hunan Provincial Natural Science(2023JJ30458).
文摘Objective To investigate whether Tuina alleviates fibrotic symptoms in myofascial trigger points(MTrPs)by regulating transforming growth factor(TGF)-β1/Smad3 signaling pathway,thereby deactivating these points.Methods This study comprised two experimental phases.In phase 1,27 specific pathogenfree(SPF)grade female Sprague-Dawley(SD)rats were randomized into three groups:control 1,model 1,and Tuina 1 groups.Model 1 and Tuina 1 groups underwent an 8-week MTrPs modeling protocol involving blunt impact and eccentric exercise.After successful modeling,rats in Tuina 1 group received manual pressing on nodules or cord-like taut bands on the medial aspect of the left hindlimb.Pain sensitivity and tissue stiffness were evaluated via pressure pain threshold(PPT)and soft tissue tension(STT).Muscle histopathology and fibrosis were observed using hematoxylin and eosin(HE)and Masson staining.Inflammatory factors in muscle were measured by enzyme-linked immunosorbent assay(ELISA),while immunofluorescence(IF)and Western blot(WB)were used to detect the expression levels ofα-smooth muscle actin(α-SMA),collagenⅢ,and TGF-β1.In phase 2,45 SPF female SD rats were randomized into five groups:control 2,model 2,Tuina 2,TGF-β1 inhibitor(TI),and Tuina+TGF-β1 agonist(Tuina+TA)groups.All groups except control 2 underwent standardized MTrPs modeling.Rats in Tuina 2 group received consistent pressing manipulation.TI group received intraperitoneal injections of oxymatrine,while Tuina+TA group received intraperitoneal injections of SRI-011381 hydrochloride followed by the same pressing protocol as Tuina 2 group.WB was used to detect the expression of collagen I,collagen III,TGF-β1,and phosphorylated-Smad3(p-Smad3)/Smad3.Results In phase 1,Tuina significantly improved PPT and STT in MTrPs of rats(P<0.01),reversed pathological damages including disorganized muscle fiber arrangement,abnormal myocyte morphology,and exacerbated fibrosis.In addition,in MTrPs of rats in model 1 group,expression levels of nuclear factor kappa-light-chain-enhancer of activated B cells(NF-κB),interleukin(IL)-1β,IL-6,tumor necrosis factor(TNF)-α,and fibrosis markers(α-SMA,collagen I,and collagen III)were upregulated,and all exhibited a significant downward trend after Tuina intervention(P<0.05 or P<0.01).This indicates that the therapeutic effects of Tuina are directly associated with reduced local inflammation and fibrosis in MTrPs.In phase 2,compared with model 2 group,rats in TI and Tuina 2 groups had decreased expression levels of TGF-β1 and p-Smad3/Smad3 in MTrPs,alongside reduced levels of inflammatory factors(IL-1β,IL-6,NF-κB,and TNF-α)and fibrosis markers(α-SMA,collagen I,and collagen III)(P<0.05 or P<0.01).When co-administered with TGF-β1 agonist,the therapeutic effects of Tuina were significantly attenuated,with rebounded TGF-β1 expression and p-Smad3/Smad3 in local MTrPs,and fibrosis and inflammatory responses were re-exacerbated(P<0.05 or P<0.01).Conclusion Tuina can effectively reduce inflammatory responses and fibrosis in MTrPs tissue,and its mechanism is closely related to the inhibition of the TGF-β1/Smad3 signaling pathway,which plays a critical role in Tuina-mediated regulation of MTrPs fibrosis.
文摘For India to achieve elimination by 2030,the challenges posed by Plasmodium(P.)vivax cannot be overlooked owing to its burden and unique biology.In 2023,in India,about 224000 malaria cases were reported,and a significant proportion(40%)were P.vivax cases.In P.vivax infection,the persistence of dormant liver stage of parasite,i.e.,hypnozoites,leading to relapses weeks or months later poses challenge in its elimination.
基金Supported by the Shenzhen Science and Technology Program(No.JCYJ20240813152704006)the National Natural Science Foundation of China(No.62401259)+2 种基金the Fundamental Research Funds for the Central Universities(No.NZ2024036)the Postdoctoral Fellowship Program of CPSF(No.GZC20242228)High Performance Computing Platform of Nanjing University of Aeronautics and Astronautics。
文摘AIM:To construct an intelligent segmentation scheme for precise localization of central serous chorioretinopathy(CSC)leakage points,thereby enabling ophthalmologists to deliver accurate laser treatment without navigational laser equipment.METHODS:A dataset with dual labels(point-level and pixel-level)was first established based on fundus fluorescein angiography(FFA)images of CSC and subsequently divided into training(102 images),validation(40 images),and test(40 images)datasets.An intelligent segmentation method was then developed,based on the You Only Look Once version 8 Pose Estimation(YOLOv8-Pose)model and segment anything model(SAM),to segment CSC leakage points.Next,the YOLOv8-Pose model was trained for 200 epochs,and the best-performing model was selected to form the optimal combination with SAM.Additionally,the classic five types of U-Net series models[i.e.,U-Net,recurrent residual U-Net(R2U-Net),attention U-Net(AttU-Net),recurrent residual attention U-Net(R2AttUNet),and nested U-Net(UNet^(++))]were initialized with three random seeds and trained for 200 epochs,resulting in a total of 15 baseline models for comparison.Finally,based on the metrics including Dice similarity coefficient(DICE),intersection over union(IoU),precision,recall,precisionrecall(PR)curve,and receiver operating characteristic(ROC)curve,the proposed method was compared with baseline models through quantitative and qualitative experiments for leakage point segmentation,thereby demonstrating its effectiveness.RESULTS:With the increase of training epochs,the mAP50-95,Recall,and precision of the YOLOv8-Pose model showed a significant increase and tended to stabilize,and it achieved a preliminary localization success rate of 90%(i.e.,36 images)for CSC leakage points in 40 test images.Using manually expert-annotated pixel-level labels as the ground truth,the proposed method achieved outcomes with a DICE of 57.13%,an IoU of 45.31%,a precision of 45.91%,a recall of 93.57%,an area under the PR curve(AUC-PR)of 0.78 and an area under the ROC curve(AUC-ROC)of 0.97,which enables more accurate segmentation of CSC leakage points.CONCLUSION:By combining the precise localization capability of the YOLOv8-Pose model with the robust and flexible segmentation ability of SAM,the proposed method not only demonstrates the effectiveness of the YOLOv8-Pose model in detecting keypoint coordinates of CSC leakage points from the perspective of application innovation but also establishes a novel approach for accurate segmentation of CSC leakage points through the“detect-then-segment”strategy,thereby providing a potential auxiliary means for the automatic and precise realtime localization of leakage points during traditional laser photocoagulation for CSC.
基金financially supported by the National Key Research and Development Program of China(2021YFF0703600)。
文摘Highlights By conjugating the same anti-N monoclonal antibody(mAb4-mAb1)with colloidal gold or fluorescent microspheres,this study developed two rapid point-of-care antigen immunochromatographic strips for the detection of porcine deltacoronavirus.The fluorescent microsphere-based lateral flow test strip demonstrated a sensitivity of 10^(1.7)TCID_(50)/0.1 mL,which is fourfold higher than that of the colloidal gold-based assay.Porcine deltacoronavirus(PDCoV)is a recently identified enteric coronavirus that causes an acute infectious disease in piglets,leading to diarrhea,vomiting,dehydration,and mortality(Hu et al.2015).
