OBJECTIVE:Antibiotics are the main drugs used to treat bacterial infections,which have been extensively utilized across various fields.However,the problem of antimicrobial resistance(AMR)has greatly limited its use,le...OBJECTIVE:Antibiotics are the main drugs used to treat bacterial infections,which have been extensively utilized across various fields.However,the problem of antimicrobial resistance(AMR)has greatly limited its use,leading to the creation of various superbugs such as Methicillin-resistant Staphylococcus aureus(MRSA),making antibiotics less effective,become a major global public health challenge.MRSA,which is resistant to mostβ-lactam and cephalosporin antibiotics,poses a significant threat to public health.Combination therapy has shown promise as a strategy to combat multi-drugresistant bacteria.Chinese medicine also has potential applications in this field.Thus,we tried to find a new approach to overcoming MRSA by combining Chinese herb and penicillin G sodium(PGS).METHODS:The antibacterial activity of Guanghuoxiang(Herba Pogostemonis)samples was investigated by turbidimetry.Then using the checkerboard assay,live/dead bacterial staining and scanning electron microscopy(SEM)to investigate whether the combination of Guanghuoxiang(Herba Pogostemonis)samples and PGS could improve the sensitivity of MRSA,and metabolomics was used to investigate the underlying mechanisms.RESULTS:In this study,we find that the Guanghuoxiang(Herba Pogostemonis)samples had good inhibitory effects on MRSA,and showed a synergistic effect when combined with PGS,enhancing the sensitivity of MRSA to PGS.Metabolomics data further revealed that this combination exerts a broader impact on the energy and material metabolism of the bacteria,resulting in improved antibacterial efficacy.CONCLUSION:Combining Guanghuoxiang(Herba Pogostemonis)with antibiotics could improve the sensitivity of drug-resistant bacteria,provided a new direction for solving the problem of AMR,and offered a valuable strategy for clinical response to MRSA.展开更多
Objective:To explore the potential active components,therapeutic targets and critical path of Alpinia officinarum and Pogostemonis Herba in the treatment of diabetic gastroparesis(DGP)by using network pharmacology.Met...Objective:To explore the potential active components,therapeutic targets and critical path of Alpinia officinarum and Pogostemonis Herba in the treatment of diabetic gastroparesis(DGP)by using network pharmacology.Methods:The main chemical components and corresponding targets genes of A.officinarum-Pogostemonis Herba were screened through TCMSP database retrieval[oral bioavailability(OB)≥30%and drug like(DL)≥0.18].Tgenes of diabetic gastroparesis were screened by the Human Gene Database(GeneCards),and Venny 2.1 software was used to obtained common targets for the active ingredients of A.officinarum-Pogostemonis Herba and DGP.Then,the protein-protein interaction(PPI)network of the common targets was constructed by STRING database and analyzed to performed the core targets.GO function and KEGG pathway enrichment analysis of the common target genes were obtained by using ClusterProfiler R package.Finally,the network diagram of"active ingredient-pathway-target"was used to establish by Cytoscape 3.8 software.Results:Totally 23 ingredients of A.officinarum-Pogostemonis Herba,97 active ingredients targets and 533 DGP related targets,including 46 common targets were selected.The common targets were mainly enriched in the cell constituents such as the nuclear chromatin and mitochondria outer membrane,involved in the biological processes as oxidative stress,apoptosis signal regulation,and molecular functions as enzyme binding,protein phosphatase binding,and cytokine activity.They were also concentrated in the signal pathways such as PI3K/Akt,HIF-1 and MAPK.The network of“active ingredients-targets-pathways”indicated the active components such as quercetin,kaempferol and galangin in A.officinarum-Pogostemonis Herba played an anti-delayed gastric emptying in diabetic gastroparesis by acting on PTGS2,NOS2,BCL2,IL6,VEGFA and other targets to jointly regulate PI3K-Akt,HIF-1 and MAPK pathways.Conclusion:This study initially reveals that the combined treatment of A.officinarum-Pogostemonis Herba for delayed gastric emptying in diabetic gastroparesis is a complex process with multi-components,multi-targets and multi-pathways,and provides a new idea for followup researches.展开更多
基金the State Key Laboratory of Drug Regulatory Science,Establishment of a Comprehensive Evaluation Method for the Quality of Chinese Medicinal Herbs Pogostemon cablin(Blanco)Benth.and Saposhnikovia divaricata(Turcz)Schischk.,Suited to the Characteristics of Traditional Chinese Medicine[No.2023SKLDRS0101]。
文摘OBJECTIVE:Antibiotics are the main drugs used to treat bacterial infections,which have been extensively utilized across various fields.However,the problem of antimicrobial resistance(AMR)has greatly limited its use,leading to the creation of various superbugs such as Methicillin-resistant Staphylococcus aureus(MRSA),making antibiotics less effective,become a major global public health challenge.MRSA,which is resistant to mostβ-lactam and cephalosporin antibiotics,poses a significant threat to public health.Combination therapy has shown promise as a strategy to combat multi-drugresistant bacteria.Chinese medicine also has potential applications in this field.Thus,we tried to find a new approach to overcoming MRSA by combining Chinese herb and penicillin G sodium(PGS).METHODS:The antibacterial activity of Guanghuoxiang(Herba Pogostemonis)samples was investigated by turbidimetry.Then using the checkerboard assay,live/dead bacterial staining and scanning electron microscopy(SEM)to investigate whether the combination of Guanghuoxiang(Herba Pogostemonis)samples and PGS could improve the sensitivity of MRSA,and metabolomics was used to investigate the underlying mechanisms.RESULTS:In this study,we find that the Guanghuoxiang(Herba Pogostemonis)samples had good inhibitory effects on MRSA,and showed a synergistic effect when combined with PGS,enhancing the sensitivity of MRSA to PGS.Metabolomics data further revealed that this combination exerts a broader impact on the energy and material metabolism of the bacteria,resulting in improved antibacterial efficacy.CONCLUSION:Combining Guanghuoxiang(Herba Pogostemonis)with antibiotics could improve the sensitivity of drug-resistant bacteria,provided a new direction for solving the problem of AMR,and offered a valuable strategy for clinical response to MRSA.
基金National Natural Science Foundation of China(81860749)。
文摘Objective:To explore the potential active components,therapeutic targets and critical path of Alpinia officinarum and Pogostemonis Herba in the treatment of diabetic gastroparesis(DGP)by using network pharmacology.Methods:The main chemical components and corresponding targets genes of A.officinarum-Pogostemonis Herba were screened through TCMSP database retrieval[oral bioavailability(OB)≥30%and drug like(DL)≥0.18].Tgenes of diabetic gastroparesis were screened by the Human Gene Database(GeneCards),and Venny 2.1 software was used to obtained common targets for the active ingredients of A.officinarum-Pogostemonis Herba and DGP.Then,the protein-protein interaction(PPI)network of the common targets was constructed by STRING database and analyzed to performed the core targets.GO function and KEGG pathway enrichment analysis of the common target genes were obtained by using ClusterProfiler R package.Finally,the network diagram of"active ingredient-pathway-target"was used to establish by Cytoscape 3.8 software.Results:Totally 23 ingredients of A.officinarum-Pogostemonis Herba,97 active ingredients targets and 533 DGP related targets,including 46 common targets were selected.The common targets were mainly enriched in the cell constituents such as the nuclear chromatin and mitochondria outer membrane,involved in the biological processes as oxidative stress,apoptosis signal regulation,and molecular functions as enzyme binding,protein phosphatase binding,and cytokine activity.They were also concentrated in the signal pathways such as PI3K/Akt,HIF-1 and MAPK.The network of“active ingredients-targets-pathways”indicated the active components such as quercetin,kaempferol and galangin in A.officinarum-Pogostemonis Herba played an anti-delayed gastric emptying in diabetic gastroparesis by acting on PTGS2,NOS2,BCL2,IL6,VEGFA and other targets to jointly regulate PI3K-Akt,HIF-1 and MAPK pathways.Conclusion:This study initially reveals that the combined treatment of A.officinarum-Pogostemonis Herba for delayed gastric emptying in diabetic gastroparesis is a complex process with multi-components,multi-targets and multi-pathways,and provides a new idea for followup researches.
