OBJECTIVE:Antibiotics are the main drugs used to treat bacterial infections,which have been extensively utilized across various fields.However,the problem of antimicrobial resistance(AMR)has greatly limited its use,le...OBJECTIVE:Antibiotics are the main drugs used to treat bacterial infections,which have been extensively utilized across various fields.However,the problem of antimicrobial resistance(AMR)has greatly limited its use,leading to the creation of various superbugs such as Methicillin-resistant Staphylococcus aureus(MRSA),making antibiotics less effective,become a major global public health challenge.MRSA,which is resistant to mostβ-lactam and cephalosporin antibiotics,poses a significant threat to public health.Combination therapy has shown promise as a strategy to combat multi-drugresistant bacteria.Chinese medicine also has potential applications in this field.Thus,we tried to find a new approach to overcoming MRSA by combining Chinese herb and penicillin G sodium(PGS).METHODS:The antibacterial activity of Guanghuoxiang(Herba Pogostemonis)samples was investigated by turbidimetry.Then using the checkerboard assay,live/dead bacterial staining and scanning electron microscopy(SEM)to investigate whether the combination of Guanghuoxiang(Herba Pogostemonis)samples and PGS could improve the sensitivity of MRSA,and metabolomics was used to investigate the underlying mechanisms.RESULTS:In this study,we find that the Guanghuoxiang(Herba Pogostemonis)samples had good inhibitory effects on MRSA,and showed a synergistic effect when combined with PGS,enhancing the sensitivity of MRSA to PGS.Metabolomics data further revealed that this combination exerts a broader impact on the energy and material metabolism of the bacteria,resulting in improved antibacterial efficacy.CONCLUSION:Combining Guanghuoxiang(Herba Pogostemonis)with antibiotics could improve the sensitivity of drug-resistant bacteria,provided a new direction for solving the problem of AMR,and offered a valuable strategy for clinical response to MRSA.展开更多
考察广藿香挥发油β-环糊精包合物的制备工艺条件.以包合物的包合率作为评价指标,选择挥发油与β-CD的比例、包合温度、超声时间为主要影响因素,进行L9(34)正交试验,优化广藿香挥发油包合物的工艺条件.同时将广藿香挥发油β-环糊精包合...考察广藿香挥发油β-环糊精包合物的制备工艺条件.以包合物的包合率作为评价指标,选择挥发油与β-CD的比例、包合温度、超声时间为主要影响因素,进行L9(34)正交试验,优化广藿香挥发油包合物的工艺条件.同时将广藿香挥发油β-环糊精包合物进行表征.结果表明:最佳包合工艺条件为挥发油与β-CD之比为1∶5、超声时间为45 m in、包合温度为50℃.在此条件下广藿香挥发油包合物的包合率可达80.9%.表征结果表明,广藿香挥发油β-环糊精包合物已经形成.因此,超声法制备广藿香挥发油的β-环糊精包合工艺合理可行.展开更多
基金the State Key Laboratory of Drug Regulatory Science,Establishment of a Comprehensive Evaluation Method for the Quality of Chinese Medicinal Herbs Pogostemon cablin(Blanco)Benth.and Saposhnikovia divaricata(Turcz)Schischk.,Suited to the Characteristics of Traditional Chinese Medicine[No.2023SKLDRS0101]。
文摘OBJECTIVE:Antibiotics are the main drugs used to treat bacterial infections,which have been extensively utilized across various fields.However,the problem of antimicrobial resistance(AMR)has greatly limited its use,leading to the creation of various superbugs such as Methicillin-resistant Staphylococcus aureus(MRSA),making antibiotics less effective,become a major global public health challenge.MRSA,which is resistant to mostβ-lactam and cephalosporin antibiotics,poses a significant threat to public health.Combination therapy has shown promise as a strategy to combat multi-drugresistant bacteria.Chinese medicine also has potential applications in this field.Thus,we tried to find a new approach to overcoming MRSA by combining Chinese herb and penicillin G sodium(PGS).METHODS:The antibacterial activity of Guanghuoxiang(Herba Pogostemonis)samples was investigated by turbidimetry.Then using the checkerboard assay,live/dead bacterial staining and scanning electron microscopy(SEM)to investigate whether the combination of Guanghuoxiang(Herba Pogostemonis)samples and PGS could improve the sensitivity of MRSA,and metabolomics was used to investigate the underlying mechanisms.RESULTS:In this study,we find that the Guanghuoxiang(Herba Pogostemonis)samples had good inhibitory effects on MRSA,and showed a synergistic effect when combined with PGS,enhancing the sensitivity of MRSA to PGS.Metabolomics data further revealed that this combination exerts a broader impact on the energy and material metabolism of the bacteria,resulting in improved antibacterial efficacy.CONCLUSION:Combining Guanghuoxiang(Herba Pogostemonis)with antibiotics could improve the sensitivity of drug-resistant bacteria,provided a new direction for solving the problem of AMR,and offered a valuable strategy for clinical response to MRSA.
文摘考察广藿香挥发油β-环糊精包合物的制备工艺条件.以包合物的包合率作为评价指标,选择挥发油与β-CD的比例、包合温度、超声时间为主要影响因素,进行L9(34)正交试验,优化广藿香挥发油包合物的工艺条件.同时将广藿香挥发油β-环糊精包合物进行表征.结果表明:最佳包合工艺条件为挥发油与β-CD之比为1∶5、超声时间为45 m in、包合温度为50℃.在此条件下广藿香挥发油包合物的包合率可达80.9%.表征结果表明,广藿香挥发油β-环糊精包合物已经形成.因此,超声法制备广藿香挥发油的β-环糊精包合工艺合理可行.
