Watermelon(Citrullus lanatus) is sensitive to salt stress. For breeding applications, it is of great significance to explore the genetic mechanism underlying salt tolerance in watermelon by analyzing the dehydration r...Watermelon(Citrullus lanatus) is sensitive to salt stress. For breeding applications, it is of great significance to explore the genetic mechanism underlying salt tolerance in watermelon by analyzing the dehydration responsive element-binding(DREB) factor family members.However, they are rarely studied in watermelon. In this study, we identified ClaDREB gene family members in watermelon based on whole genome data;analyzed the physicochemical properties, evolution, and phylogeny;and studied their expression patterns under salt stress in two watermelon varieties with varying salt tolerance. In total, 57 DREB family members were identified in watermelon, and most of them were located in the nucleus. ClaDREBs were divided into six subgroups Ⅰ-Ⅵ. The promoter region of ClaDREBs from subgroup Ⅱ contained many defense-related and stress responsive elements. Among them, ClaDREB14 was significantly upregulated by salt stress and exhibited differential expression in salt-tolerant and salt-sensitive varieties. Moreover, overexpression of ClaDREB14 in watermelon roots significantly improved the salt tolerance of transgenic plants;mainly, it significantly increased the activities of POD, SOD, and CAT and significantly reduced MDA content.However, the results from gene-edited watermelon roots obtained using CRISPR/Cas9 vectors showed the opposite trend. Furthermore, we demonstrated that ClaDREB14 directly binds to the cis-acting element ACCGAC in the promoter region of ClaPOD6 and promotes its expression.Therefore, ClaDREB14 may enhance salt tolerance by increasing the activity of antioxidant enzymes in watermelon roots. This study provided valuable information on the DREB gene family in watermelon and laid the foundation for future functional validation and genetic engineering applications.展开更多
The common vetch(Vicia sativa L.)is a self-pollinated annual forage legume that is widely distributed worldwide.It has wide adaptability and high nutritional value and is commonly used as an important protein source f...The common vetch(Vicia sativa L.)is a self-pollinated annual forage legume that is widely distributed worldwide.It has wide adaptability and high nutritional value and is commonly used as an important protein source for livestock feed.However,pod shattering seriously limits the yield of common vetch.To clarify the mechanism of pod shattering in common vetch,the pod walls of three shattering-resistant(SR)accessions(B65,B135,and B392)and three shattering-susceptible(SS)accessions(L33,L170,and L461)were selected for transcriptome sequencing.A total of 17,190 differentially expressed genes(DEGs)were identified in the pod wall of B135 and L461 common vetch at 5,10,15,20,and 25 days after anthesis.Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis showed that“phenylpropanoid biosynthesis”was the most significantly enriched pathway,and 40 structural genes associated with lignin biosynthesis were identified and differentially expressed in B135 and L461 common vetch.We analysed the DEGs in the pod wall of three SR and three SS accessions at 15 days after anthesis,and most of the DEGs were consistent with the significant enrichment pathways identified in B135 and L461 common vetch.The total lignin content of SR accessions was significantly lower than the SS accessions.The present study lays a foundation for understanding the molecular regulatory mechanism of pod shattering related to lignin biosynthesis in common vetch and provides reference functional genes for breeders to further cultivate shattering-resistant common vetch varieties.展开更多
The study focuses on the filling of pods from ten cacao clones originating from French Guiana in Côte d’Ivoire. Two reference clones (IFC5 and NA32) were used as controls. The parameters studied include the numb...The study focuses on the filling of pods from ten cacao clones originating from French Guiana in Côte d’Ivoire. Two reference clones (IFC5 and NA32) were used as controls. The parameters studied include the number of ovules per ovary, the average number of beans per pod, the fresh bean weight, and the pod index. The aim was to evaluate the genetic variability and agronomic performance of these clones under natural pollination conditions. The results show significant genetic variability between the clones for all traits studied. The IFC5 clone, known for its excellent pod filling, exhibited the highest apparent fertility (0.91), while the GU183/A clone had the lowest filling (0.47). Three types of distributions of the number of beans per pod were identified: a right-skewed unimodal distribution, characteristic of well-filled clones;a left-skewed unimodal distribution, associated with poorly filled clones;and an intermediate bimodal distribution. These differences could be related to pollination compatibility and the availability of compatible pollen. Regarding the fresh bean weight and pod index, the GU183/A clone stood out with the heaviest beans (3.27 g) but had a high pod index (49.58), indicating relatively low productivity. None of the Guyanese clones achieved the filling level of the IFC5 clone, although some surpassed the NA32 control. This study highlights the importance of apparent fertility and the number of beans per pod as essential criteria for cacao genetic improvement programs. The authors recommend extending research to a larger number of Guyanese clones and exploring complementary traits, such as the influence of pollination type and intergroup compatibility.展开更多
基金funded by grants fromthe China Agriculture Research System of MOF and MARA(CARS-25)the Key Research and Development Program of Xinjiang Uygur autonomous region(Grant No.2023B02017)+3 种基金the Agricultural Science and Technology Innovation Program(CAAS-ASTIP-2021-ZFRI,CAAS-ASTIP-2024-WRI)the Basic Research Funds of Chinese Academy of Agricultural Sciences(Grant No.1610192023201)Natural Science Foundation of Henan Province(Grant No.252300421694)Joint Research on Agricultural Variety Improvement of Henan Province(Grant No.2022010503).
文摘Watermelon(Citrullus lanatus) is sensitive to salt stress. For breeding applications, it is of great significance to explore the genetic mechanism underlying salt tolerance in watermelon by analyzing the dehydration responsive element-binding(DREB) factor family members.However, they are rarely studied in watermelon. In this study, we identified ClaDREB gene family members in watermelon based on whole genome data;analyzed the physicochemical properties, evolution, and phylogeny;and studied their expression patterns under salt stress in two watermelon varieties with varying salt tolerance. In total, 57 DREB family members were identified in watermelon, and most of them were located in the nucleus. ClaDREBs were divided into six subgroups Ⅰ-Ⅵ. The promoter region of ClaDREBs from subgroup Ⅱ contained many defense-related and stress responsive elements. Among them, ClaDREB14 was significantly upregulated by salt stress and exhibited differential expression in salt-tolerant and salt-sensitive varieties. Moreover, overexpression of ClaDREB14 in watermelon roots significantly improved the salt tolerance of transgenic plants;mainly, it significantly increased the activities of POD, SOD, and CAT and significantly reduced MDA content.However, the results from gene-edited watermelon roots obtained using CRISPR/Cas9 vectors showed the opposite trend. Furthermore, we demonstrated that ClaDREB14 directly binds to the cis-acting element ACCGAC in the promoter region of ClaPOD6 and promotes its expression.Therefore, ClaDREB14 may enhance salt tolerance by increasing the activity of antioxidant enzymes in watermelon roots. This study provided valuable information on the DREB gene family in watermelon and laid the foundation for future functional validation and genetic engineering applications.
基金supported by the Leading Scientist Project of Qinghai Province,China(2023-NK-147)。
文摘The common vetch(Vicia sativa L.)is a self-pollinated annual forage legume that is widely distributed worldwide.It has wide adaptability and high nutritional value and is commonly used as an important protein source for livestock feed.However,pod shattering seriously limits the yield of common vetch.To clarify the mechanism of pod shattering in common vetch,the pod walls of three shattering-resistant(SR)accessions(B65,B135,and B392)and three shattering-susceptible(SS)accessions(L33,L170,and L461)were selected for transcriptome sequencing.A total of 17,190 differentially expressed genes(DEGs)were identified in the pod wall of B135 and L461 common vetch at 5,10,15,20,and 25 days after anthesis.Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis showed that“phenylpropanoid biosynthesis”was the most significantly enriched pathway,and 40 structural genes associated with lignin biosynthesis were identified and differentially expressed in B135 and L461 common vetch.We analysed the DEGs in the pod wall of three SR and three SS accessions at 15 days after anthesis,and most of the DEGs were consistent with the significant enrichment pathways identified in B135 and L461 common vetch.The total lignin content of SR accessions was significantly lower than the SS accessions.The present study lays a foundation for understanding the molecular regulatory mechanism of pod shattering related to lignin biosynthesis in common vetch and provides reference functional genes for breeders to further cultivate shattering-resistant common vetch varieties.
文摘The study focuses on the filling of pods from ten cacao clones originating from French Guiana in Côte d’Ivoire. Two reference clones (IFC5 and NA32) were used as controls. The parameters studied include the number of ovules per ovary, the average number of beans per pod, the fresh bean weight, and the pod index. The aim was to evaluate the genetic variability and agronomic performance of these clones under natural pollination conditions. The results show significant genetic variability between the clones for all traits studied. The IFC5 clone, known for its excellent pod filling, exhibited the highest apparent fertility (0.91), while the GU183/A clone had the lowest filling (0.47). Three types of distributions of the number of beans per pod were identified: a right-skewed unimodal distribution, characteristic of well-filled clones;a left-skewed unimodal distribution, associated with poorly filled clones;and an intermediate bimodal distribution. These differences could be related to pollination compatibility and the availability of compatible pollen. Regarding the fresh bean weight and pod index, the GU183/A clone stood out with the heaviest beans (3.27 g) but had a high pod index (49.58), indicating relatively low productivity. None of the Guyanese clones achieved the filling level of the IFC5 clone, although some surpassed the NA32 control. This study highlights the importance of apparent fertility and the number of beans per pod as essential criteria for cacao genetic improvement programs. The authors recommend extending research to a larger number of Guyanese clones and exploring complementary traits, such as the influence of pollination type and intergroup compatibility.
