In this study cortisol and cyclophosphamide were injected into abdominal cavities of mice to keep them in an immunosuppressive state. Then the pathogen Klebsiella pneumoniae was given to infect the lungs and the exper...In this study cortisol and cyclophosphamide were injected into abdominal cavities of mice to keep them in an immunosuppressive state. Then the pathogen Klebsiella pneumoniae was given to infect the lungs and the experimental modcl of lobular pneumonia in the mice was thus established successfully.The experimental animal model has typical pathologic manifestation and good replication and can be used to to study the effect of drugs.展开更多
We established a diagnostic model to predict acute Mycoplasma pneumoniae （M. pneumonia） infection in elderly Community-acquired pneumonia （CAP） patients. We divided 456 patients into acute and non-acute M. pneumon...We established a diagnostic model to predict acute Mycoplasma pneumoniae （M. pneumonia） infection in elderly Community-acquired pneumonia （CAP） patients. We divided 456 patients into acute and non-acute M. pneumoniae infection groups. Binary logistic regression and receiver operating characteristic （ROC） curves were used to establish a predictive model. The following independent factors were identified： age 〉 70 years; serum cTNT level 〉 0.0S ng/mL; lobar consolidation; mediastinal lymphadenopathy; and antibody titer in the acute phase 〉 1：40. The area under the ROC curve of the model was 0.923 and a score of 2 7 score predicted acute M. pneumoniae infection in elderly patients with CAP. The predictive model developed in this study has high diagnostic accuracy for the identification of elderly acute M. pneumoniae infection.展开更多
To better predict the spread of the COVID-19 outbreak, mathematical modeling and analysis of the spread of the COVID-19 outbreak is proposed based on data analysis and infectious disease theory. Firstly, the mathemati...To better predict the spread of the COVID-19 outbreak, mathematical modeling and analysis of the spread of the COVID-19 outbreak is proposed based on data analysis and infectious disease theory. Firstly, the mathematical model indicators of the spread of the new coronavirus pneumonia epidemic are determined by combining the theory of infectious diseases, the basic assumptions of the spread model of the new coronavirus pneumonia epidemic are given based on the theory of data analysis model, the spread rate of the new coronavirus pneumonia epidemic is calculated by combining the results of the assumptions, and the spread rate of the epidemic is inverted to push back into the assumptions to complete the construction of the mathematical modeling of the diffusion. Relevant data at different times were collected and imported into the model to obtain the spread data of the new coronavirus pneumonia epidemic, and the results were analyzed and reflected. The model considers the disease spread rate as the dependent variable of temperature, and analyzes and verifies the spread of outbreaks over time under real temperature changes. Comparison with real results shows that the model developed in this paper is more in line with the real disease spreading situation under specific circumstances. It is hoped that the accurate prediction of the epidemic spread can provide relevant help for the effective containment of the epidemic spread.展开更多
Objective To understand the molecular basis for a potential reaction mechanism and develop novel antibiotics with homology modeling for 3-hydroxy-3-methylglutaryl coenzyme A （HMG-CoA） synthase （HMGS）. Methods The ...Objective To understand the molecular basis for a potential reaction mechanism and develop novel antibiotics with homology modeling for 3-hydroxy-3-methylglutaryl coenzyme A （HMG-CoA） synthase （HMGS）. Methods The genetic engineering technology and the composer module of SYBYL7.0 program were used, while the HMGS three-dimensional structure was analyzed by homology modeling. Results The mvaS gene was cloned from Streptococcus pneumoniae and overexpressed in Escherichia coli from a pET28 vector. The expressed enzyme （about 46 kDa） was purified by affinity chromatography with a specific activity of 3.24 μmol/min/mg. Optimal conditions were pH 9.75 and 10 mmol/L MgCl2 at 37 ℃ The Vmax and Km were 4.69 μmol/min/mg and 213 μmol/L respectively. The 3D model of S.pneumoniae HMGS was established based on structure template of HMGS of Enterococcus faecalis. Conelusion The structure of HMGS will facilitate the structure-based design of alternative drugs to cholesterol-lowering therapies or to novel antibiotics to the Gram-positive cocci, whereas the recombinant HMGS will prove useful for drug development against a different enzyme in the mevalonate pathway.展开更多
文摘In this study cortisol and cyclophosphamide were injected into abdominal cavities of mice to keep them in an immunosuppressive state. Then the pathogen Klebsiella pneumoniae was given to infect the lungs and the experimental modcl of lobular pneumonia in the mice was thus established successfully.The experimental animal model has typical pathologic manifestation and good replication and can be used to to study the effect of drugs.
基金supported by the Capital Medical Development and Scientific Research Fund(2009-1033)and the Science and Technology Plan of Beijing City(Z101107050210018)
文摘We established a diagnostic model to predict acute Mycoplasma pneumoniae （M. pneumonia） infection in elderly Community-acquired pneumonia （CAP） patients. We divided 456 patients into acute and non-acute M. pneumoniae infection groups. Binary logistic regression and receiver operating characteristic （ROC） curves were used to establish a predictive model. The following independent factors were identified： age 〉 70 years; serum cTNT level 〉 0.0S ng/mL; lobar consolidation; mediastinal lymphadenopathy; and antibody titer in the acute phase 〉 1：40. The area under the ROC curve of the model was 0.923 and a score of 2 7 score predicted acute M. pneumoniae infection in elderly patients with CAP. The predictive model developed in this study has high diagnostic accuracy for the identification of elderly acute M. pneumoniae infection.
文摘To better predict the spread of the COVID-19 outbreak, mathematical modeling and analysis of the spread of the COVID-19 outbreak is proposed based on data analysis and infectious disease theory. Firstly, the mathematical model indicators of the spread of the new coronavirus pneumonia epidemic are determined by combining the theory of infectious diseases, the basic assumptions of the spread model of the new coronavirus pneumonia epidemic are given based on the theory of data analysis model, the spread rate of the new coronavirus pneumonia epidemic is calculated by combining the results of the assumptions, and the spread rate of the epidemic is inverted to push back into the assumptions to complete the construction of the mathematical modeling of the diffusion. Relevant data at different times were collected and imported into the model to obtain the spread data of the new coronavirus pneumonia epidemic, and the results were analyzed and reflected. The model considers the disease spread rate as the dependent variable of temperature, and analyzes and verifies the spread of outbreaks over time under real temperature changes. Comparison with real results shows that the model developed in this paper is more in line with the real disease spreading situation under specific circumstances. It is hoped that the accurate prediction of the epidemic spread can provide relevant help for the effective containment of the epidemic spread.
基金supported by the National Natural Science Foundation of China (No. 30771429)Science and Technology Research Project of Ministry of Education (No.106116)+1 种基金Specialized Research Fund for the Doctoral Program of Higher Education (No. 20060511002)the Natural Science Foundation of Hubei Province (No. 2006ABA197)
文摘Objective To understand the molecular basis for a potential reaction mechanism and develop novel antibiotics with homology modeling for 3-hydroxy-3-methylglutaryl coenzyme A （HMG-CoA） synthase （HMGS）. Methods The genetic engineering technology and the composer module of SYBYL7.0 program were used, while the HMGS three-dimensional structure was analyzed by homology modeling. Results The mvaS gene was cloned from Streptococcus pneumoniae and overexpressed in Escherichia coli from a pET28 vector. The expressed enzyme （about 46 kDa） was purified by affinity chromatography with a specific activity of 3.24 μmol/min/mg. Optimal conditions were pH 9.75 and 10 mmol/L MgCl2 at 37 ℃ The Vmax and Km were 4.69 μmol/min/mg and 213 μmol/L respectively. The 3D model of S.pneumoniae HMGS was established based on structure template of HMGS of Enterococcus faecalis. Conelusion The structure of HMGS will facilitate the structure-based design of alternative drugs to cholesterol-lowering therapies or to novel antibiotics to the Gram-positive cocci, whereas the recombinant HMGS will prove useful for drug development against a different enzyme in the mevalonate pathway.
文摘目的系统评价ICU机械通气患者呼吸机相关性肺炎(ventilator-associated pneumonia,VAP)发生风险的预测模型,为临床医护人员开发或选择合适的风险预测模型提供参考。方法系统检索中国知网、万方数据库、维普期刊库、中国生物医学文献数据库、PubMed、EMbase、Web of Science、CINAHL和Cochrane Library中截至2025年4月30日发表的相关文献。2名研究者独立筛选文献与提取数据,并进行质量评价。结果共纳入21篇文献,涉及27个预测模型,受试者工作特征曲线特征下面积为0.722~1.000。质量评价结果显示,21篇文献总体偏倚风险较高,13篇文献适用性较好,偏倚风险主要源于未选择合适的数据来源、样本量不足、自变量和缺失数据处理不当、仅采用单因素分析筛选预测因子、模型性能评估不全。重复报告出现频次较高的预测因子是ICU住院时间、呼吸机使用天数、急性生理学和慢性健康状况评分系统Ⅱ评分、气管切开、联合使用抗生素。结论现有的ICU机械通气患者VAP发生风险预测模型的预测性能较好,但偏倚风险较高。未来研究者应重点关注研究设计的方法学细节及报告的规范性,以提高模型性能,便于临床推广。
