Objective:To evaluate the antileishmanial activity of different extracts from three Cuban Pluchea species.Methods:In in vitro assays the IC<sub>50</sub>was calculated in the promastigotes and amastigotes f...Objective:To evaluate the antileishmanial activity of different extracts from three Cuban Pluchea species.Methods:In in vitro assays the IC<sub>50</sub>was calculated in the promastigotes and amastigotes forms as cytotoxicity in murine macrophages.In leishmaniasis cutanea experiment,mortality, weight loss,lesion size and burden parasite were measured.Results:Extracts evaluated showed inhibitive effect on growing of promastigote form;however,active extracts caused a high toxicity. Ethanol and n-hexane extracts demonstrated specific antileishmanial activity.Ethanol and n-hexane extracts from Pluchea carolinensis（P.carolinensis） caused similar inhibition against amastigote form.The intraperitoneal administration of the ethanol extract of P.carolinensis at 100 mg/kg prevented lesion development compared with control groups.Conclusions:The antileishmanial experiment suggests that ethanol extracts from P.carolinensis is the most promising.Further studies are still needed to evaluate the polential of this plant as a source of new antileishmanial agents.展开更多
文摘Objective:To evaluate the antileishmanial activity of different extracts from three Cuban Pluchea species.Methods:In in vitro assays the IC<sub>50</sub>was calculated in the promastigotes and amastigotes forms as cytotoxicity in murine macrophages.In leishmaniasis cutanea experiment,mortality, weight loss,lesion size and burden parasite were measured.Results:Extracts evaluated showed inhibitive effect on growing of promastigote form;however,active extracts caused a high toxicity. Ethanol and n-hexane extracts demonstrated specific antileishmanial activity.Ethanol and n-hexane extracts from Pluchea carolinensis（P.carolinensis） caused similar inhibition against amastigote form.The intraperitoneal administration of the ethanol extract of P.carolinensis at 100 mg/kg prevented lesion development compared with control groups.Conclusions:The antileishmanial experiment suggests that ethanol extracts from P.carolinensis is the most promising.Further studies are still needed to evaluate the polential of this plant as a source of new antileishmanial agents.
