Different concentrations of jasmonic acid(JA)and benzothiadiazole(BTH) were sprayed on 2-year-old Rosa rugosa‘Plena’ seedlings. The induced resistance of JA and BTH to Sphaerotheca pannosa(Wallr.) and the changes of...Different concentrations of jasmonic acid(JA)and benzothiadiazole(BTH) were sprayed on 2-year-old Rosa rugosa‘Plena’ seedlings. The induced resistance of JA and BTH to Sphaerotheca pannosa(Wallr.) and the changes of their related physiological indices were investigated. Results showed that JA and BTH treatments had inhibitory impacts on S. pannosa infection. The optimal concentration of JA and BTH was 0.5 mmol/L for the disease-resistance induction of the leaves, its inductive effect was up to 66.36% for BTH and 54.49% for JA. Our results confirmed that exogenous JA and BTH significantly improved R. rugose ‘Plena’ resistance to S. pannosa. When treated with JA and BTH, activities of the three defense enzymes(POD, PPO, and PAL) increased significantly.Contents of total phenolics, flavonoids, and lignin also increased significantly. It is inferred from these results that exogenous JA and BTH could improve the resistance of R.rugose ‘Plena’ to S. pannosa through enhancing activities of the defensive enzymes and accumulation of secondary metabolites in the leaves.展开更多
To study the effect of jasmonates(JAs)on the resistance of economic forest plants to insects,R osa rugosa‘Plena'leaves were treated with 1 mmol/L jasmonic acid(JA),methyl jasmonate(MeJA)and Z-jasmone,then the con...To study the effect of jasmonates(JAs)on the resistance of economic forest plants to insects,R osa rugosa‘Plena'leaves were treated with 1 mmol/L jasmonic acid(JA),methyl jasmonate(MeJA)and Z-jasmone,then the content of tannin and total phenol in leaves and the feeding area of Monolepta hieroglyphica adults on leaves were measured.Changes in the activities of detoxification enzymes in adult M.hieroglyphica that had fed on leaves treated with JAs were also studied.Tannin and total phenol levels in leaves increased significantly after treatment with JAs,and tannin level was 1.36–1.55-fold higher than in the control after treatment with 1 mmol/L MeJA.The total content of phenol in leaves treated with 1.0 mmol/L Z-jasmone increased by 1.33–2.20-fold compared with those of the control.The activities of detoxification enzymes in adults were inhibited to differing degrees:activity of alkaline phosphatase(AKP)first increased,then decreased;the activities of acid phosphatase(ACP),glutathione S-transferases(GSTs)and carboxylesterase(CarE)following treatment with 1 mmol/L MeJA were significantly reduced and were 22%–31%,11%–26%,and 11%–31%lower than those of the control,respectively.Moreover,the feeding area of adult M.hieroglyphica on the leaves treated with JAs was significantly reduced(P<0.05).The feeding area of economic forest R.rugosa‘Plena'leaves treated with 1 mmol/L MeJA decreased by 17%–43%compared with that of the control.Moreover,the decrease in the adult M.hieroglyphica feeding area was highly positively correlated with the content of tannin and positively correlated with the contents of total phenol of economic forest R.rugosa‘Plena'leaves.The reduced feeding area of adult M.hieroglyphica was highly negatively correlated with the activities of AKP and ACP and negatively correlated with those of the GSTs.In conclusion,the use of 1 mmol/L MeJA can noticeably decrease the deleterious effects of adult M.hieroglyphica.展开更多
Objective： To investigate the anti-proliferative effects of saponins prepared from Plena Clematis （PC） cultured in Fujian Province, China on 4 human tumor cell lines and its possible anti-tumor mechanism. Methods：...Objective： To investigate the anti-proliferative effects of saponins prepared from Plena Clematis （PC） cultured in Fujian Province, China on 4 human tumor cell lines and its possible anti-tumor mechanism. Methods： The growth inhibition assays of saponins on human esophageal squamous carcinoma cell line （EC9706）, human hepatoma cell line （HepG-2）, human oral cancer cell line （KB） and human gastric cancer cell line （BGC-823） were evaluated in vitro by thiazolyl blue （MTT） method. The inhibitory effects on EC9706 treated with different concentrations of saponins （15.62, 31.25, 62.50, 125, 250 and 500 μg/mL） were performed in vitro by MTT method. The morphology and nuclear staining with acridine orange/ethidium bromide of EC9706 calls treated with saponins were illustrated under an inverted phase fluorescence microscope. The apoptotic effects of saponins were further evaluated by annexin-V/propidium iodide dual staining experiment to examine the occurrence of phosphatidylserine externalization onto the call surface by a flow cytometer. Results： MTT assay showed that the saponins could inhibit the proliferation of 4 tumor cell lines. Among them, the maximum inhibition rate of 73.1% was detected in EC9706 cells at the saponins concentration of 250 μg/mL for 24 h. Further investigation indicated that the saponins induced EC9706 cells apoposis. The EC9706 cells presented apoptotic characteristics when treated with saponins, including that the morphologies of EC9706 cells were appeared round-shaped with higher refraction, and the cell nuclear stained orange with EB after 250 μ g/mL saponins exposure. The flow cytometry analysis results showed that the induction of cell cycle arrest in apoptotic system may participate in the anti-proliferative activity of saponins on EC9706 cells. Conclusion： The saponins from PC exhibited significant cytotoxicity against human EC9706, KB, BGC-823, and HepG-2 cells and might be beneficial to development of ethnic pharmaceutical plant for potential anti-tumor drugs.展开更多
基金supported by the Science Foundation of Heilongjiang Province of China(No.QC2014C012)the Fundamental Research Funds for the Central Universities(NO.2572016CA11)
文摘Different concentrations of jasmonic acid(JA)and benzothiadiazole(BTH) were sprayed on 2-year-old Rosa rugosa‘Plena’ seedlings. The induced resistance of JA and BTH to Sphaerotheca pannosa(Wallr.) and the changes of their related physiological indices were investigated. Results showed that JA and BTH treatments had inhibitory impacts on S. pannosa infection. The optimal concentration of JA and BTH was 0.5 mmol/L for the disease-resistance induction of the leaves, its inductive effect was up to 66.36% for BTH and 54.49% for JA. Our results confirmed that exogenous JA and BTH significantly improved R. rugose ‘Plena’ resistance to S. pannosa. When treated with JA and BTH, activities of the three defense enzymes(POD, PPO, and PAL) increased significantly.Contents of total phenolics, flavonoids, and lignin also increased significantly. It is inferred from these results that exogenous JA and BTH could improve the resistance of R.rugose ‘Plena’ to S. pannosa through enhancing activities of the defensive enzymes and accumulation of secondary metabolites in the leaves.
基金financially by the National Natural Science Foundation of China(No.31800546)the National Key R&D Program of China(No.2018YFC1200400)the Fundamental Research Funds for the Central Universities(No.2572016CA11)。
文摘To study the effect of jasmonates(JAs)on the resistance of economic forest plants to insects,R osa rugosa‘Plena'leaves were treated with 1 mmol/L jasmonic acid(JA),methyl jasmonate(MeJA)and Z-jasmone,then the content of tannin and total phenol in leaves and the feeding area of Monolepta hieroglyphica adults on leaves were measured.Changes in the activities of detoxification enzymes in adult M.hieroglyphica that had fed on leaves treated with JAs were also studied.Tannin and total phenol levels in leaves increased significantly after treatment with JAs,and tannin level was 1.36–1.55-fold higher than in the control after treatment with 1 mmol/L MeJA.The total content of phenol in leaves treated with 1.0 mmol/L Z-jasmone increased by 1.33–2.20-fold compared with those of the control.The activities of detoxification enzymes in adults were inhibited to differing degrees:activity of alkaline phosphatase(AKP)first increased,then decreased;the activities of acid phosphatase(ACP),glutathione S-transferases(GSTs)and carboxylesterase(CarE)following treatment with 1 mmol/L MeJA were significantly reduced and were 22%–31%,11%–26%,and 11%–31%lower than those of the control,respectively.Moreover,the feeding area of adult M.hieroglyphica on the leaves treated with JAs was significantly reduced(P<0.05).The feeding area of economic forest R.rugosa‘Plena'leaves treated with 1 mmol/L MeJA decreased by 17%–43%compared with that of the control.Moreover,the decrease in the adult M.hieroglyphica feeding area was highly positively correlated with the content of tannin and positively correlated with the contents of total phenol of economic forest R.rugosa‘Plena'leaves.The reduced feeding area of adult M.hieroglyphica was highly negatively correlated with the activities of AKP and ACP and negatively correlated with those of the GSTs.In conclusion,the use of 1 mmol/L MeJA can noticeably decrease the deleterious effects of adult M.hieroglyphica.
基金Supported by the Medical Elite Cultivation Program of Fujian Province,China(No.2016-ZQN-75)the Medical Innovation Subject of Fujian Province,China(No.2012-CX-5)
文摘Objective： To investigate the anti-proliferative effects of saponins prepared from Plena Clematis （PC） cultured in Fujian Province, China on 4 human tumor cell lines and its possible anti-tumor mechanism. Methods： The growth inhibition assays of saponins on human esophageal squamous carcinoma cell line （EC9706）, human hepatoma cell line （HepG-2）, human oral cancer cell line （KB） and human gastric cancer cell line （BGC-823） were evaluated in vitro by thiazolyl blue （MTT） method. The inhibitory effects on EC9706 treated with different concentrations of saponins （15.62, 31.25, 62.50, 125, 250 and 500 μg/mL） were performed in vitro by MTT method. The morphology and nuclear staining with acridine orange/ethidium bromide of EC9706 calls treated with saponins were illustrated under an inverted phase fluorescence microscope. The apoptotic effects of saponins were further evaluated by annexin-V/propidium iodide dual staining experiment to examine the occurrence of phosphatidylserine externalization onto the call surface by a flow cytometer. Results： MTT assay showed that the saponins could inhibit the proliferation of 4 tumor cell lines. Among them, the maximum inhibition rate of 73.1% was detected in EC9706 cells at the saponins concentration of 250 μg/mL for 24 h. Further investigation indicated that the saponins induced EC9706 cells apoposis. The EC9706 cells presented apoptotic characteristics when treated with saponins, including that the morphologies of EC9706 cells were appeared round-shaped with higher refraction, and the cell nuclear stained orange with EB after 250 μ g/mL saponins exposure. The flow cytometry analysis results showed that the induction of cell cycle arrest in apoptotic system may participate in the anti-proliferative activity of saponins on EC9706 cells. Conclusion： The saponins from PC exhibited significant cytotoxicity against human EC9706, KB, BGC-823, and HepG-2 cells and might be beneficial to development of ethnic pharmaceutical plant for potential anti-tumor drugs.
