Fieldwork along several segments of the Altyn Tagh Fault,between 85 and 95°E,confirms that it ranks as one of the most active faults of Asia.In the East,near Aksay,the active fault trace offsets numerous stream c...Fieldwork along several segments of the Altyn Tagh Fault,between 85 and 95°E,confirms that it ranks as one of the most active faults of Asia.In the East,near Aksay,the active fault trace offsets numerous stream channels,terrace risers and fans tens to hundreds of meters. 14 C dating of organic remains and charcoal within terrace gravels indicates that most of the terraces were emplaced after the beginning of the Holocene,implying a left\|slip rate of about 2cm/a.Large mole tracks attest to the occurrence of great earthquakes.Even larger mole tracks are found north of Lenghu,within the Altun Shan push\|up,a 6000 m high range in a restraining bend of the fault,now sliced by its most active strand.North of Huatougou,at the transition between another push\|up mountain and a recent pull\|apart basin,a spectacular sequence of five flat\|floored,hanging channels,beheaded by the fault from a unique source in the mountain,have been horizontally displaced by up to 1250m.Cosmogenic dating of the abandonment of these channels and of nearby offset terrace risers confirms the slip\|rate at Aksay.Several km to the west,pressure ridges exceeding 10m in height across a large young fan,imply the repeat of several great earthquakes in a relatively short time span.展开更多
A pair of oligonucleotide primers were designed to amplify the endoflagella gene of L. Interrogans serovar lai. An approximately 840 bp fragment was generated with PCR and inserted into plasmid pUC8,after the fragment...A pair of oligonucleotide primers were designed to amplify the endoflagella gene of L. Interrogans serovar lai. An approximately 840 bp fragment was generated with PCR and inserted into plasmid pUC8,after the fragment and pUC8 were digested respectively with BamHI and PstI. A recombinant plasmid (designated as pLF1 ) was obtained. SDS-PAGE analysis indicated that a 33 kDa was expressed in E. Coli JM103 harboring pLF1 and the expression level of the protein was 11 % of the total bacterial soluble proteins. Western blot analysis showed that the protein band could be recognized by the antiserum against the endoflegella (Axial filament ) of Leptospira interrt,gans serover lai. Nucleotide sequence data showed an open reading frame encoding 282 aminoacids residues,corresponding to a protein of molecular weight 33. 6kDa. Comparison of the deduced endoflagellar subunit protein (flaB) amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Trehoema pallid um flaB proteins. Immunization/protection experiment was performed on the model of BALB/C mice and showed that there was higher survival rate in the group JM103-pLE1 than in the group JM103-pUC8.展开更多
文摘Fieldwork along several segments of the Altyn Tagh Fault,between 85 and 95°E,confirms that it ranks as one of the most active faults of Asia.In the East,near Aksay,the active fault trace offsets numerous stream channels,terrace risers and fans tens to hundreds of meters. 14 C dating of organic remains and charcoal within terrace gravels indicates that most of the terraces were emplaced after the beginning of the Holocene,implying a left\|slip rate of about 2cm/a.Large mole tracks attest to the occurrence of great earthquakes.Even larger mole tracks are found north of Lenghu,within the Altun Shan push\|up,a 6000 m high range in a restraining bend of the fault,now sliced by its most active strand.North of Huatougou,at the transition between another push\|up mountain and a recent pull\|apart basin,a spectacular sequence of five flat\|floored,hanging channels,beheaded by the fault from a unique source in the mountain,have been horizontally displaced by up to 1250m.Cosmogenic dating of the abandonment of these channels and of nearby offset terrace risers confirms the slip\|rate at Aksay.Several km to the west,pressure ridges exceeding 10m in height across a large young fan,imply the repeat of several great earthquakes in a relatively short time span.
文摘A pair of oligonucleotide primers were designed to amplify the endoflagella gene of L. Interrogans serovar lai. An approximately 840 bp fragment was generated with PCR and inserted into plasmid pUC8,after the fragment and pUC8 were digested respectively with BamHI and PstI. A recombinant plasmid (designated as pLF1 ) was obtained. SDS-PAGE analysis indicated that a 33 kDa was expressed in E. Coli JM103 harboring pLF1 and the expression level of the protein was 11 % of the total bacterial soluble proteins. Western blot analysis showed that the protein band could be recognized by the antiserum against the endoflegella (Axial filament ) of Leptospira interrt,gans serover lai. Nucleotide sequence data showed an open reading frame encoding 282 aminoacids residues,corresponding to a protein of molecular weight 33. 6kDa. Comparison of the deduced endoflagellar subunit protein (flaB) amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Trehoema pallid um flaB proteins. Immunization/protection experiment was performed on the model of BALB/C mice and showed that there was higher survival rate in the group JM103-pLE1 than in the group JM103-pUC8.
