Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HP...Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.展开更多
Objective:This study aimed to elucidate the active components and mechanisms of Plantago asiatica L.(PAL)in the treatment of gout.Materials and Methods:Based on its polarity,PAL was partitioned into three parts,P1,P2,...Objective:This study aimed to elucidate the active components and mechanisms of Plantago asiatica L.(PAL)in the treatment of gout.Materials and Methods:Based on its polarity,PAL was partitioned into three parts,P1,P2,and P3,through gradient elution using AB-8 macroporous resin columns.The primary constituents of these fractions include iridoids,phenylethanoid glucosides,and flavonoids.Subsequently,a comprehensive in vitro and in vivo screening and evaluation model was developed considering the fundamental pathological processes of gout,including the inhibition of uric acid(UA)production,augmentation of UA excretion,and anti-inflammatory effects.This model was used to analyze the anti-gout activity of P1,P2,P3,and the overall extracts derived from PAL.Results:All the PAL parts exhibited specific therapeutic properties as part of gout therapy.Specifically,the P2 part demonstrated the ability to diminish interleukin-1 beta levels,lower UA concentrations,suppress joint inflammation in rats,and restore abnormal parameters associated with gout,such as creatinine and urea nitrogen.These findings highlight the significant effect of P2 on gout treatment.Conclusions:Following a thorough assessment of the outcomes derived from diverse anti-gout experiments,P2 exhibited superior anti-gout properties compared to the remaining parts.P2 demonstrated a restorative effect on the aberrant parameters associated with gout,thereby comprehensively elucidating the therapeutic mechanism of P2 against gout.This study provides substantial evidence and serves as a reference for future investigations regarding the material foundation of PAL in gout treatment.展开更多
Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-...Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-butanol-ethanol-water （0.5：0.5：0.1：1, v/v/v/v）. A total of 45.6 mg of compound 1 and 293.8 mg of compound 2 were purified from 1341 mg of the n-butanol extract of P. asiatica. The structures of the two PhGs were tentatively identified as plantamajoside and aeteoside or isoacteoside by eleetrospray ionization multi stage tandem mass spectrometry （ESI-MS^n） in the negative ion mode.展开更多
Understanding polysaccharide-gut microbe interactions is critical for elucidating their health benefits for the host.This study aimed to investigate the interaction between polysaccharides from Plantago asiatica L.see...Understanding polysaccharide-gut microbe interactions is critical for elucidating their health benefits for the host.This study aimed to investigate the interaction between polysaccharides from Plantago asiatica L.seeds(PLCP)and Bacteroides by using antibiotics induced pseudo-sterile mice that gavaged with Bacteroides strains for 5 days and then switched to 7 days of PLCP,based on the changes of gut microbiota,short-chain fatty acids(SCFAs)and serum biochemical parameters.Seven Strains from different species with good PLCP utilization ability successfully survived in the gut of these mice.The additional PLCP tended to significantly and differently impact the relative abundance of Bacteroides only in B.thetaiotaomicron C1 and B.salyersiae C13 treated mice(0.05<P<0.1).LEfSe analysis further suggested strain-specific interaction consequence with PLCP on microbiota.Notably,B.koreensis C16 and B.salyersiae C13 significantly elevated total colonic SCFAs levels,respectively,mainly due to the increase in acetic acid,which were not affected by further PLCP treatment.PLCP significantly decreased propionic-and increased butyric acid levels in mice recieving B.koreensis C16,while the former was also seen in B.fragilis C11 and the latter in B.salyersiae C13 treated ones.Moreover,only B.salyersiae C13+PLCP treatment significantly elevated serum total protein,albumin,and HDL-C levels in mice.These findings establish a theoretical framework for elucidating the mechanisms underlying PLCP-Bacteroides in-teractions,providing fundamental insights for future mechanistic studies and therapeutic development targeting gut microbiota.展开更多
[Objectives]To explore the pharmacodynamic material basis of Sijicao granules for the treatment of eczema through chemical composition-network pharmacology.[Methods]First of all,the chemical constituents of Polygonum ...[Objectives]To explore the pharmacodynamic material basis of Sijicao granules for the treatment of eczema through chemical composition-network pharmacology.[Methods]First of all,the chemical constituents of Polygonum capitatum and Plantago asiatica from Sijicao granules were collected,and the relevant target information of the constituents was collected by TCMSP,PubChem,DisGeNET,GeneCards and STRING databases.Furthermore,Cytoscape 3.8.2 software was used to construct the chemical compounds-target network map of Sijicao granules.Finally,STRING database was used for PPI protein network analysis,GO functional enrichment analysis and KEGG pathway enrichment analysis of core targets,and molecular docking between core constituents and protein targets was also performed.[Results]30 constituents,including quercetin,kaempferol,luteolin,ellagic acid and gallic acid,were discovered to be the key effective compounds of Sijicao granules in the treatment of eczema.And its core action protein targets were PTGS2,NOS2,AKT1,TP53,IL6,HMOX1.What s more,through GO functional enrichment analysis of biological process(BP),cell component(CC),molecular function(MF)analysis and KEGG pathway enrichment analysis,the main pathways of action of Sijicao granules for the treatment of eczema including IL-17 signaling pathway,T cell receptor signaling pathway,cancer signaling pathway,TNF signaling pathway and Relaxin signaling pathway.In addition,molecular docking results displayed that the primary active constituents quercetin,kaempferol and luteolin were well combined with the core protein targets AKT1 and IL6.[Conclusions]Sijicao granules could play an important role for the treatment of eczema through multi-component,multi-target,multi-pathway and their interaction.展开更多
基金Supported by the National Natural Science Foundation of China(No.30873364)the Natural Science Foundation of Jilin Province of China(Nos.20071102 and 2008-167)
文摘Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.
基金the support of the National Natural Science Foundation of China(No.8187296 Authors 9)the Science and Youth Innovation Promotion Association of the CAS(Grant No.2019227)。
文摘Objective:This study aimed to elucidate the active components and mechanisms of Plantago asiatica L.(PAL)in the treatment of gout.Materials and Methods:Based on its polarity,PAL was partitioned into three parts,P1,P2,and P3,through gradient elution using AB-8 macroporous resin columns.The primary constituents of these fractions include iridoids,phenylethanoid glucosides,and flavonoids.Subsequently,a comprehensive in vitro and in vivo screening and evaluation model was developed considering the fundamental pathological processes of gout,including the inhibition of uric acid(UA)production,augmentation of UA excretion,and anti-inflammatory effects.This model was used to analyze the anti-gout activity of P1,P2,P3,and the overall extracts derived from PAL.Results:All the PAL parts exhibited specific therapeutic properties as part of gout therapy.Specifically,the P2 part demonstrated the ability to diminish interleukin-1 beta levels,lower UA concentrations,suppress joint inflammation in rats,and restore abnormal parameters associated with gout,such as creatinine and urea nitrogen.These findings highlight the significant effect of P2 on gout treatment.Conclusions:Following a thorough assessment of the outcomes derived from diverse anti-gout experiments,P2 exhibited superior anti-gout properties compared to the remaining parts.P2 demonstrated a restorative effect on the aberrant parameters associated with gout,thereby comprehensively elucidating the therapeutic mechanism of P2 against gout.This study provides substantial evidence and serves as a reference for future investigations regarding the material foundation of PAL in gout treatment.
基金supported by funding from Jilin Province (No.20060904-1 and 20071102).
文摘Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-butanol-ethanol-water （0.5：0.5：0.1：1, v/v/v/v）. A total of 45.6 mg of compound 1 and 293.8 mg of compound 2 were purified from 1341 mg of the n-butanol extract of P. asiatica. The structures of the two PhGs were tentatively identified as plantamajoside and aeteoside or isoacteoside by eleetrospray ionization multi stage tandem mass spectrometry （ESI-MS^n） in the negative ion mode.
基金support from National Natural Science Fund for Excellent Young Scholars(32222065)National Key Research and Development Program of China(2023YFF1103600)+3 种基金Key Research and Development Projects of Jiangxi Province(20244AFH82001)Key Research and Development Project of Jiangxi Province(20223BBF61023)National Key Research and Development Program of China(2023YFF1105400)The Central Guidance Fund for Local Science and Technology Development in Jiangxi Province(20252ZDD020002)were gratefully acknowledged.
文摘Understanding polysaccharide-gut microbe interactions is critical for elucidating their health benefits for the host.This study aimed to investigate the interaction between polysaccharides from Plantago asiatica L.seeds(PLCP)and Bacteroides by using antibiotics induced pseudo-sterile mice that gavaged with Bacteroides strains for 5 days and then switched to 7 days of PLCP,based on the changes of gut microbiota,short-chain fatty acids(SCFAs)and serum biochemical parameters.Seven Strains from different species with good PLCP utilization ability successfully survived in the gut of these mice.The additional PLCP tended to significantly and differently impact the relative abundance of Bacteroides only in B.thetaiotaomicron C1 and B.salyersiae C13 treated mice(0.05<P<0.1).LEfSe analysis further suggested strain-specific interaction consequence with PLCP on microbiota.Notably,B.koreensis C16 and B.salyersiae C13 significantly elevated total colonic SCFAs levels,respectively,mainly due to the increase in acetic acid,which were not affected by further PLCP treatment.PLCP significantly decreased propionic-and increased butyric acid levels in mice recieving B.koreensis C16,while the former was also seen in B.fragilis C11 and the latter in B.salyersiae C13 treated ones.Moreover,only B.salyersiae C13+PLCP treatment significantly elevated serum total protein,albumin,and HDL-C levels in mice.These findings establish a theoretical framework for elucidating the mechanisms underlying PLCP-Bacteroides in-teractions,providing fundamental insights for future mechanistic studies and therapeutic development targeting gut microbiota.
基金Supported by Science and Technology Planning Project of Guizhou Province[QKHJC-ZK(2022)362]Science and Technology Achievements Transfer and Transformation Project of Guizhou Provincial Department of Education[(2022)064]+1 种基金Higher Education Institution Engineering Research Center of Guizhou Provincial Department of Education[(2023)035,No.(2023)zk01]National Undergraduate Innovation Training Project(202210660131,202310660082).
文摘[Objectives]To explore the pharmacodynamic material basis of Sijicao granules for the treatment of eczema through chemical composition-network pharmacology.[Methods]First of all,the chemical constituents of Polygonum capitatum and Plantago asiatica from Sijicao granules were collected,and the relevant target information of the constituents was collected by TCMSP,PubChem,DisGeNET,GeneCards and STRING databases.Furthermore,Cytoscape 3.8.2 software was used to construct the chemical compounds-target network map of Sijicao granules.Finally,STRING database was used for PPI protein network analysis,GO functional enrichment analysis and KEGG pathway enrichment analysis of core targets,and molecular docking between core constituents and protein targets was also performed.[Results]30 constituents,including quercetin,kaempferol,luteolin,ellagic acid and gallic acid,were discovered to be the key effective compounds of Sijicao granules in the treatment of eczema.And its core action protein targets were PTGS2,NOS2,AKT1,TP53,IL6,HMOX1.What s more,through GO functional enrichment analysis of biological process(BP),cell component(CC),molecular function(MF)analysis and KEGG pathway enrichment analysis,the main pathways of action of Sijicao granules for the treatment of eczema including IL-17 signaling pathway,T cell receptor signaling pathway,cancer signaling pathway,TNF signaling pathway and Relaxin signaling pathway.In addition,molecular docking results displayed that the primary active constituents quercetin,kaempferol and luteolin were well combined with the core protein targets AKT1 and IL6.[Conclusions]Sijicao granules could play an important role for the treatment of eczema through multi-component,multi-target,multi-pathway and their interaction.
