Moringa oleifera(MO)is traditionally used to mitigate inflammatory-mediated disorders;however,the influence of ecotypic variation on its anti-inflammatory activity remains poorly understood.In this study,we compared t...Moringa oleifera(MO)is traditionally used to mitigate inflammatory-mediated disorders;however,the influence of ecotypic variation on its anti-inflammatory activity remains poorly understood.In this study,we compared the phytochemical composition and anti-inflammatory activity of ethanolic extracts obtained from fresh and dried leaves of four MO ecotypes(India,Paraguay,Mozambique,and Pakistan),all grown under the same outdoor conditions,as well as two commercial powders(Just Moringa and WISSA),using LPS-stimulated RAW 264.7 macrophages.Extracts from fresh leaves were 19-43%more cytotoxic than those from dried leaves,depending on the ecotype,likely due to higher cyanogenic glycoside content.Extracts from the India and Paraguay ecotypes,characterized by high levels of quercetin derivatives and caffeic acids,as well as Just Moringa,enriched in kaempferol derivatives,significantly inhibited LPS-induced nitric oxide(NO)production(p<0.05).Just Moringa and Paraguay extracts also reduced iNOS gene expression(p<0.05 and p<0.01,respectively),whereas only the Paraguay extract decreased iNOS protein levels(p<0.05).In contrast,quercetin-3-O-glucoside and rutin showed significant effects only at concentrations approximately 100-fold higher than those present in the extracts,indicating that the phytocomplex displays greater bioactivity than individual compounds.Overall,these results demonstrate that ecotypic variation strongly affects the polyphenolic composition and anti-inflammatory properties of MO leaves,highlighting the importance of reporting both origin and phytochemical composition in MO-based products.展开更多
Background: Plant-based diets are eating patterns focusing on high intake of plant-basedfoods while limiting the intake of animal-based foods. In recent decades, plant-based dietshave received increasing attention for...Background: Plant-based diets are eating patterns focusing on high intake of plant-basedfoods while limiting the intake of animal-based foods. In recent decades, plant-based dietshave received increasing attention for their potential benefits for humans and theenvironment. However, a comprehensive overview of the development trends and researchhotspots of plant-based diets is still lacking. Therefore, this scientometric study aimed to fillthis critical research gap. Methods: English literature related to plant-based diets publishedfrom 2012 to 2023 in Science Citation Index Expanded (SCIE) of Web of Science CoreCollection was collected on August 16, 2023, using relevant key terms. R-bibliometrix,CiteSpace, and VOSviewer were used to analyze data and construct knowledge graphs.Results: Overall, 4135 publications (3127 articles and 1008 reviews) were included in thisstudy, showing an obvious upward trend in this field annually from 2012 to 2023, rangingfrom 70 to 921. The USA published the highest number of studies (n =1158) and was theleading research center in this field worldwide. Universities were the main researchinstitutions;particularly, Harvard T.H. Chan School of Public Health contributed to thelargest number of publications (n = 88) and had an active cooperation with otherinstitutions. Mcclements DJ contributed the most publications (n = 49) and ranked firstamong the scholars in plant-based diets. The most productive academic journal (n = 353)on plant-based diets was Nutrients, with an IF2022 of 5.9, while Foods and Frontiers in Nutritionwere the relatively active journals in this field in recent years. “Quality,” “milk,” “culturedmeat,” “plant protein,” “environmental impacts,” “functional properties,” “plant-based meatalternative,” “dietary change,” “vegan diet,” “plant-based beverage,” “gut microbiota,”“middle-income countries,” and “chronic kidney disease” were the main research hotspotsand frontiers in the field of plant-based diets. Conclusion: This study provides acomprehensive overview of global research on plant-based diets. Although research in thisfield has gained increasing attention in recent decades, cooperation among differentinstitutions and researchers should be significantly strengthened. Moreover, more studiesare needed to further assess the health and environmental impacts of plant-based diets.展开更多
Background:Ampelopsis grossedentata,vine tea,which is the tea alternative beverages in China.In vine tea processing,a large amount of broken tea is produced,which has low commercial value.Methods:This study investigat...Background:Ampelopsis grossedentata,vine tea,which is the tea alternative beverages in China.In vine tea processing,a large amount of broken tea is produced,which has low commercial value.Methods:This study investigates the influence of different extraction methods(room temperature water extraction,boiling water extraction,ultrasonic-assisted room temperature water extraction,and ultrasonic-assisted boiling water extraction,referred to as room temperature water extraction(RE),boiling water extraction(BE),ultrasonic assistance at room temperature water extraction(URE),and ultrasonic assistance in boiling water extraction(UBE))on the yield,dihydromyricetin(DMY)content,free amino acid composition,volatile aroma components,and antioxidant properties of vine tea extracts.Results:A notable influence of extraction temperature on the yield of vine tea extracts(P<0.05),with BE yielding the highest at 43.13±0.26%,higher than that of RE(34.29±0.81%).Ultrasound-assisted extraction significantly increased the DMY content of the extracts(P<0.05),whereas DMY content in the RE extracts was 59.94±1.70%,that of URE reached 66.14±2.78%.Analysis revealed 17 amino acids,with L-serine and aspartic acid being the most abundant in the extracts,nevertheless ultrasound-assisted extraction reduced total free amino acid content.Gas chromatography-mass spectrometry analysis demonstrated an increase in the diversity and quantity of compounds in the vine tea water extracts obtained through ultrasonic-assisted extraction.Specifically,69 and 68 volatile compounds were found in URE and UBE extracts,which were higher than the number found in RE and BE extracts.In vitro,antioxidant activity assessments revealed varying antioxidant capacities among different extraction methods,with RE exhibiting the highest DPPH scavenging rate,URE leading in ABTS•+free radical scavenging,and BE demonstrating superior ferric ion reducing antioxidant activity.Conclusion:The findings suggest that extraction methods significantly influence the chemical composition and antioxidant properties of vine tea extracts.Ultrasonic-assisted extraction proved instrumental in elevating the DMY content in vine tea extracts,thereby enriching its flavor profile while maintaining its antioxidant properties.展开更多
Plant-based milk is rich in polyunsaturated fatty acids,polyphenols and other bioactive compounds.This study investigated the effect of 3 plant-based milk(flaxseed milk,oat milk and soy milk)on the ceftriaxone-induced...Plant-based milk is rich in polyunsaturated fatty acids,polyphenols and other bioactive compounds.This study investigated the effect of 3 plant-based milk(flaxseed milk,oat milk and soy milk)on the ceftriaxone-induced intestinal disorders,and compared the regulation patterns associated with gut microbiome and metabolome.The results showed plant-based milk alleviated the ceftriaxone caused cecum swelling,colonic tissue damage and intestinal microecological disorders.Meanwhile,administered plant-based milk decreased levels of pro-inflammatory cytokine(tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β)and oxidative stresses(malondialdehyde(MDA)and myeloperoxidase(MPO)in the colon,as well as increasing the levels of tight junction proteins(Occludin,Claudin-1,and ZO-1)in the colon.Moreover,administration of plant-based milk modulated the intestinal microbiota by promoting the relative levels of beneficial bacteria(Bifidobacterium),and inhibiting the harmful bacteria genus(Enterococcus).Furthermore,plant-based milk treatment significantly modulated glycerophospholipids metabolism(e.g.glycerophosphocholine)and arachidonic acid metabolism(e.g.prostaglandin G2 and arachidonic acid)in the serum.In conclusion,plant-based milk could alleviate antibiotic-related imbalance of barrier function damage,gut microbiota disorders and the reduction of metabolic disorders,which lays a foundation for exploring anti-inflammatory and intestinal micro-ecological approach to plant-based milk.展开更多
Plant-based milks are on the rise due to an increased awareness of their sustainability and health benefits.Currently,dairy milk is the most nutritionally complete beverage,but it suffers from the presence of indigest...Plant-based milks are on the rise due to an increased awareness of their sustainability and health benefits.Currently,dairy milk is the most nutritionally complete beverage,but it suffers from the presence of indigestible lactose and allergenic proteins.Coconut milk has been around for a long time,but its application is limited due to a perceived lack of specific nutrients,high saturated fat levels,and low acceptability.Recent evidence indicates,however,that the saturated fat and other plant-based components found in coconut milk are good for metabolic outcomes and brain health.The conversion of coconut milk to yoghurt will further improve its functionality by boosting its existing nutritional qualities.In this article,the nutritional value of coconut milk,as well as its potential downsides,its application as yoghurt,and suggestions for enhancing its nutritional functionality will be examined.展开更多
Consumers are increasingly demanding natural colorants that are safe and offer health benefits.In addition to their ornamental characteristics,Kanzan cherry(KC)blossoms present a promising source of red-hued natural c...Consumers are increasingly demanding natural colorants that are safe and offer health benefits.In addition to their ornamental characteristics,Kanzan cherry(KC)blossoms present a promising source of red-hued natural colorants and functional bioactive substances.This research utilized distilled water to extract KC petals(KCP)and their ground powders(KCPP)under varying temperatures(30℃–90℃)and times(30–180 min).The total monomeric anthocyanins(TMAC)and total phenolics(TPC)in the extracts were evaluated via the pH differential and Folin–Ciocalteu methods.Antioxidant capacities were assessed by DPPH free radical scavenging ability and reducing power.Results indicated that the optimal extraction of TMAC and TPC from KCP occurred at 90℃ for 30 min,and the resulting extracts exhibited the highest antioxidant activities among all tested temperatures and durations.Compared to different particle sizes,the finest KCPP generally produced extracts with the highest TMAC,TPC,and antioxidant activity,due to enhanced mass and heat transfer.When compared with the acidified alcohol method,hot water extraction resulted in 68.23%and 71.41%TMAC yields for petals and powders,respectively,while TPC levels were similar or higher.TMAC or TPC showed a significantly positive correlation(p<0.01)with the antioxidant activities.These findings demonstrate that hot water extraction is a viable and environmentally friendly alternative for phytochemical recovery from KC.Additionally,elevated extraction temperature and pH accelerated anthocyanin degradation and shortened its half-life,while higher pH also lowered the activation energy,enthalpy,entropy,and Gibbs free energy.Thus,red–orange KC extracts with rich bioactivity may serve as promising ingredients for functional foods having acidic pH levels.展开更多
Traditional Chinese Medicine(TCM)has been utilized in China for thousands of years to treat and prevent various diseases,with recent research highlighting microRNAs(miRNAs)in herbal decoctions as key bioactive compone...Traditional Chinese Medicine(TCM)has been utilized in China for thousands of years to treat and prevent various diseases,with recent research highlighting microRNAs(miRNAs)in herbal decoctions as key bioactive components.The oral lyophilized powder of the pig spleen extracts(PAT)composed of oligonucleotides and peptides extracted from fresh pig spleen and delivered via a lipid mixture containing neutral cytidinyl lipid DNCA and cationic lipid CLD(Mix),has demonstrated significant antitumor effects against A549 and BCPAP cells,promoting early and late apoptosis by approximately 20%in A549 cells.Intratumoral administration of PAT/Mix in A549-bearing mice significantly reduced both tumor volume and weight.Transcriptomic and proteomic analyses suggested that PAT/Mix exerted its antitumor activity by silencing DEPDC1 mRNA in A549 cells,thereby influencing cellular processes and metabolic pathways.Additionally,PAT/Mix was found to promote the maturation of bone marrow dendritic cells(BMDCs),enhancing antigen processing and presentation.These findings indicated that PAT/Mix held promise as a therapeutic agent with both immunomodulatory and antitumor properties,owing not only to its peptide components but also to miRNAs,which played a critical therapeutic role.展开更多
[Objectives]Using Calamine Zinc Oxide Phytocomplex Cream as an example,this study employed network pharmacology to investigate the therapeutic potential and mechanism of action of the combination of calamine,zinc oxid...[Objectives]Using Calamine Zinc Oxide Phytocomplex Cream as an example,this study employed network pharmacology to investigate the therapeutic potential and mechanism of action of the combination of calamine,zinc oxide,and plant extracts in eczema intervention.[Methods]Active constituents of Calamine Zinc Oxide Phytocomplex Cream were identified through screening using the HIT2.0,HERB,and TCMSP databases.Corresponding targets of the active constituents were predicted using NetInfer.The collected targets were intersected with eczema and atopic dermatitis(AD)-related targets obtained from the GeneCards database to identify the effective therapeutic targets of Calamine Zinc Oxide Phytocomplex Cream.The network diagram of effective active constituents versus therapeutic targets for Calamine Zinc Oxide Phytocomplex Cream was constructed and subjected to topological analysis using Cytoscape software.The Protein-Protein Interaction(PPI)network was established and analyzed using the String database,Cytoscape software,and the cytoHubba plugin to identify key hub genes.Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed on the therapeutic targets using the DAVID database.[Results]Screening identified 57 active constituents in Calamine Zinc Oxide Phytocomplex Cream,corresponding to 601 potential targets.Subsequent analysis revealed 72 core therapeutic targets of Calamine Zinc Oxide Phytocomplex Cream specifically relevant to eczema and AD.Analysis of the network diagram suggested that Calamine Zinc Oxide Phytocomplex Cream may exert anti-inflammatory and immunomodulatory effects through active constituents such as quercetin,luteolin,and apigenin,while concurrently repairing skin barrier function by acting on targets including AKT1,NF-κB,and STAT3.Furthermore,the inclusion of mineral-based medicines provides additional functions such as itch relief and reinforcement of the skin barrier.[Conclusions]Calamine Zinc Oxide Phytocomplex Cream combines organic and inorganic constituents,synergistically alleviating the adverse symptoms of eczema and AD through multiple pathways.展开更多
Background:Fruits of Indian gooseberry or Amla or Aonla(Phyllanthus emblica)are important ingredients in many Ayurvedic medicines,but little is known about its leaves.Methods:Different extracts and crude alkaloids of ...Background:Fruits of Indian gooseberry or Amla or Aonla(Phyllanthus emblica)are important ingredients in many Ayurvedic medicines,but little is known about its leaves.Methods:Different extracts and crude alkaloids of P.emblica leaves were evaluated for their antimicrobial activity against clinically important microbes using agar well diffusion assay.The antimicrobial activity of methanolic extract(ME)of P.emblica leaves was also compared with similarly prepared methanolic extracts from leaves of Yellow Kaner(Cascabela peruviana),Parijaat or Harsingar(Nyctanthes arbor-tristis),Custard apple(Annona squamosa),Garlic vine(Mansoa alliacea),Shami plant(Prosopis cineraria),Madar(Calotropis gigantea),and Bael(Aegle marmelos).Results:The ME of leaves of P.emblica was the most potent preparation against bacteria and yeast.Of the 338 strains of microbes belonging to 100 species(96 bacteria and four Candida species),the ME of P.emblica inhibited the growth of 300 strains.A total of 84.62%,96.39%,and 100%of 221 G−bacteria,111 G+bacteria,and 6 Candida species strains,respectively,were inhibited by ME of P.emblica leaves at≤36 mg/well.The aqueous extract of P.emblica leaves also inhibited a similar number of bacterial strains,but at higher concentrations,while the ether extract could inhibit only staphylococci.The alkaloid from P.emblica leaves and the ME from leaves of other plants had insignificant antimicrobial activity at similar≤36 mg/well concentration.Conclusion:The study concluded that the ME of P.emblica leaves may be a useful source of a potent,wide-spectrum antimicrobial substance(s).展开更多
BACKGROUND The rising global burden of liver diseases,such as non-alcoholic fatty liver disease and liver fibrosis,has necessitated innovative therapeutic approaches.Plant-based therapies,recognized for their anti-inf...BACKGROUND The rising global burden of liver diseases,such as non-alcoholic fatty liver disease and liver fibrosis,has necessitated innovative therapeutic approaches.Plant-based therapies,recognized for their anti-inflammatory and antioxidant properties,have shown promising effects.However,poor bioavailability limits their clinical application.AIM To map global research trends,key contributors,and emerging themes in plant-based therapies combined with advanced drug delivery systems for liver health.METHODS Using the Scopus database,645 documents were retrieved and analyzed using bibliometric tools Biblioshiny and VOSviewer.Analysis focused on publication trends,geographical contributions,and advancements in drug delivery technologies,including nanoparticles,liposomes,and polymeric micelles.Metrics such as publication growth rate,authorship collaboration,and thematic clustering were assessed.RESULTS The dataset spans 43 years(1981-2024),with an annual growth rate of 11.09%in the number of publications.Research output is dominated by China(33%),followed by the United States(24%)and India(18%).Collaborative studies accounted for 24.34%of publications,with an average of 5.81 co-authors per document.Key innovations include nanoparticle encapsulation of curcumin and silymarin,improving bioavailability by up to 85%.Highly cited studies demonstrated the antioxidant,anti-inflammatory,and anti-fibrotic properties of these compounds.For instance,curcumin nanoparticles showed a 70%improvement in solubility,and silymarin liposomal formulations enhanced therapeutic efficiency by 62%.Thematic analysis revealed a transition from basic clinical observations to molecular and pharmacokinetic research,with a focus on oxidative stress mitigation and hepatoprotection.CONCLUSION This study highlights the growing synergy between plant-based therapies and advanced drug delivery systems,with significant contributions from Asian and Western countries.Future efforts should prioritize clinical trials,standardization of plant extract formulations,and interdisciplinary approaches to maximize therapeutic outcomes.The findings provide a foundation for integrating plant-derived compounds into evidence-based hepatological therapies,addressing critical challenges in bioavailability and safety.展开更多
Gilia capitata Sims(Polemoniaceae)is an annual herbaceous plant widely distributed in western North America and cultivated as an ornamental flower.The detailed chemical composition and pharmacological properties of th...Gilia capitata Sims(Polemoniaceae)is an annual herbaceous plant widely distributed in western North America and cultivated as an ornamental flower.The detailed chemical composition and pharmacological properties of this plant have not been previously reported.This study aimed to investigate the chemical composition and pharmacological activity of aqueous-ethanolic extracts derived from different plant parts(herb,flowers,stems,roots,and fruits).HPLC analysis identified 12 phenolic compounds and 16 amino acids in the aqueous-ethanolic extracts obtained from these plant parts.The biological effects of the extracts were evaluated in vitro,including cytotoxicity,anti-inflammatory response,andwound-healing potential.The anti-inflammatory activity was evaluated by measuring their effect on NO production in LPS-stimulated RAW264.7 murine macrophages and HaCaT keratinocytes.Phagocytic activity was assessed using the neutral red uptake assay.HaCaT cell proliferation and migration were assessed to evaluate the wound-healing potential.Morphological changes in both cell lines were examined microscopically.Moreover,neurobehavioral effects were studied in vivo.The herb extracts significantly enhanced locomotor activity while preserving exploratory behaviour,suggesting a stimulating effect.G.capitata flower extracts caused a decrease in both locomotor and exploratory behaviour,demonstrating a moderate sedative effect.Fruit extracts significantly suppressed locomotor and exploratory activity,indicating a strong sedative effect.These findings indicated that G.capitata contains bioactive compounds with diverse pharmaceutical effects depending on the specific plant part and may have potential applications in medicine and pharmacology pending further research.展开更多
Anoectochilus roxburghii from different planting sources was used as the research object,and the biological activity of its extracts was compared.The results showed that the whole plant extracts of A.roxburghii plante...Anoectochilus roxburghii from different planting sources was used as the research object,and the biological activity of its extracts was compared.The results showed that the whole plant extracts of A.roxburghii planted in bag culture (BAG),greenhouse cultivation (GRE) and tissue culture seedlings (TIS) were rich in active ingredients such as A.roxburghii polysaccharides and A.roxburghii glycosides.In vitro experiments showed that Anoectochilus roxburghii extracts from different planting sources had good antioxidant capacity,tyrosinase inhibition ability and hyaluronidase inhibition ability.Among them,the GRE Anoectochilus roxburghii whole plant extract had the strongest scavenging ability on DPPH free radical,ABTS free radical and hydroxyl free radical,and the inhibitory effect on tyrosinase activity and hyaluronidase activity was significantly higher than that of BAG and TIS.At the same time,it also verified that the extract of Anoectochilus roxburghii has antioxidant,whitening,anti-inflammatory and other skin care effects,which provides a theoretical basis for the development and application of Anoectochilus roxburghii extract in the field of cosmetics.展开更多
The valorization of Amazonian wood residues into active chemical compounds could be an eco-friendly,cost-effective and valuable way to develop wood preservative formulations to enhance the decay and termite resistance...The valorization of Amazonian wood residues into active chemical compounds could be an eco-friendly,cost-effective and valuable way to develop wood preservative formulations to enhance the decay and termite resistance of low-durable wood species.Wacapou(Vouacapoua americana.,Fabaceae)is a well-known Guianese wood spe-cies commonly used in local wood construction due to its outstanding natural durability,which results from the presence of a large panel of extractives compounds.In addition,its industrial processing generates large amounts of residues.Wacapou residues were extracted by maceration using four different solvents(water/ethanol,ethyl acetate,hexane and dichloromethane/methanol),separately and successively.The yield of each extractive fraction was determined,and their chemical compositions were analyzed by Liquid Chromatography-Mass Spectrometry(LC-MS).Ethyl acetate led to the highest extraction yield,and the active compounds were identified in the obtained extractive fraction.In this sense,the fungicidal and termite-repellent properties of these extractives were then tested using a screening laboratory(with temperate and tropical microorganisms),according to the solution concentration(1%,2.5%,5%,8%and 10%).Finally,Virola michelii Heckel wood samples(low durable species)were impregnated with the 8%concentration solution.The impregnated wood samples were then exposed to a soil bed test.The results highlighted that the nature of the solvent used during wood maceration affects the con-tent of the obtained extractive fractions.Ultra-Performance Liquid Chromatography–High-Resolution Mass Spectrometry(UHPLC-HRMS)analyses showed the influence of extraction parameters on the nature of the extracted molecules.Wacapou extracts(from ethyl acetate maceration)showed good anti-fungal and anti-termite activities.Additionally,the concentration in extractives had an impact on the anti-termite activity level for Reti-culitermesflavipes and Cryptotermes sp.Formulations based on Wacapou extractives showed a good potential for valorization in eco-friendly preservatives,aiming to confer better durability to local low-durability wood species.展开更多
Periodontitis is an inflammatory infectious disease affecting the periodontal supporting tissues and is the primary cause of tooth loosening and tooth loss in adults.Clinically,supragingival scaling,subgingival scalin...Periodontitis is an inflammatory infectious disease affecting the periodontal supporting tissues and is the primary cause of tooth loosening and tooth loss in adults.Clinically,supragingival scaling,subgingival scaling,root planing,and other basic periodontal treatments,often combined with antibiotic therapy,are commonly employed with moderate efficacy.However,the increasing prevalence of antibiotic resistance and associated adverse reactions has become a growing concern.Recent studies have demonstrated the significant impact of traditional Chinese medicine(TCM)extracts in both the prevention and treatment of periodontitis,exhibiting remarkable effectiveness.This review explores the role and mechanisms of TCM extracts in the prevention and treatment of periodontitis,providing a reference for further elucidation of their mechanisms and a theoretical basis for the development of Chinese herbal medicine-based care products.展开更多
Standard bacterial suspensions play a crucial role in microbiological diagnosis.Traditional prepar-ation methods,which rely heavily on manual operations,face challenges such as poor reproducibility,low ef-ficiency,and...Standard bacterial suspensions play a crucial role in microbiological diagnosis.Traditional prepar-ation methods,which rely heavily on manual operations,face challenges such as poor reproducibility,low ef-ficiency,and biosafety concerns.In this study,we propose a high-precision automated colony extraction and separation system that combines large-field imaging and artificial intelligence(AI)to facilitate intelligent screening and localization of colonies.Firstly,a large-field imaging system was developed to capture high-resolution images of 90 mm Petri dishes,achieving a physical resolution of 13.2μm and an imaging speed of 13 frames per second.Subsequently,AI technology was employed for the automatic recognition and localiza-tion of colonies,enabling the selection of target colonies with diameters ranging from 1.9 to 2.3 mm.Next,a three-axis motion control platform was designed,accompanied by a path planning algorithm for the efficient extraction of colonies.An electronic pipette was employed for accurate colony collection.Additionally,a bacterial suspension concentration measurement module was developed,incorporating a 650 nm laser diode as the light source,achieving a measurement accuracy of 0.01 McFarland concentration(MCF).Finally,the system’s performance was validated through the preparation of an Esckerichia coli(E.coli)suspension.After 17 hours of cultivation,E.coli was extracted four times,achieving the target concentration set by the system.This work is expected to enable rapid and accurate microbial sample preparation,significantly reducing de-tection cycles and alleviating the workload of healthcare personnel.展开更多
The goal of this study was to synthesize nickel oxide nanoparticles(NiO-NPs)by the sol-gel method,which involved the use of salvia macrosiphon Boiss plant extract,Ni(NO3)2·6 H2 O as a capping agent,and a nickel p...The goal of this study was to synthesize nickel oxide nanoparticles(NiO-NPs)by the sol-gel method,which involved the use of salvia macrosiphon Boiss plant extract,Ni(NO3)2·6 H2 O as a capping agent,and a nickel precursor,respectively.The synthesized NiO-NPs were characterized by Fourier transform infrared spectroscopy(FTIR),ultraviolet-visible(UV-Vis)spectrophotometer,X-ray diffraction(XRD),field electron scanning electron microscopy(FESEM)/energy-dispersive X-ray spectroscopy(EDX),thermo-gravimetric analysis/differential thermal analysis(TGA/DTA)and vibrating sample magnetometer(VSM)analyzes.Also,according to the results of UV-Vis,the gap band of nanoparticles was calculated to be in the range of about 2.9-3.9 eV.The photocatalytic activity of nanoparticles on methylene blue(MB)degradation was investigated and according to the results,about 80%MB was apparently degraded in the presence of NiO-NPs under UV-A light(11 W)after 5 h in pH^11.We have evaluated the cytotoxicity of NiO-NPs on the multiple tumor cells by materials method,and all the utilized concentrations were observed to cause non-toxic effects;therefore,it can be suggested that these samples have the potential of being employed in different fields of medicine.展开更多
The recycling of neptunium(Np)from nuclear wastes is crucial for the sustainable development of nuclear energy,yet it is still a challenging task owing to the complexity of Np chemistry.Precise control of oxidation st...The recycling of neptunium(Np)from nuclear wastes is crucial for the sustainable development of nuclear energy,yet it is still a challenging task owing to the complexity of Np chemistry.Precise control of oxidation state is highly desirable for the effective recovery of Np.In this study,we report an innovative strategy for Np recovery through in-situ coordination and reduction of Np(Ⅴ)in a biphasic extraction system.By leveraging the synergistic effects of coordination by a P=O donating ligand(trialkyl phosphine oxide,TRPO)and reduction by hydroquinone(HQ)in the organic phase,efficient Np(Ⅴ)-to-Np(Ⅳ)conversion and high distribution ratio(D)of Np were achieved in a single extraction contact.The reduction mechanism of Np was elucidated through spectroscopic and theoretical analyses.This work enriches the redox chemistry of Np and provides a novel pathway for Np recovery in advanced nuclear fuel cycles.展开更多
BACKGROUND Ischemic heart disease ranks among the foremost contributors to mortality worldwide.Myocardial infarction injury poses a prevalent challenge in current therapies.Studies have shown that mesenchymal stem cel...BACKGROUND Ischemic heart disease ranks among the foremost contributors to mortality worldwide.Myocardial infarction injury poses a prevalent challenge in current therapies.Studies have shown that mesenchymal stem cell transplantation increases cytokine release,reduces myocardial cell necrosis,and improves left ventricular function;thus,it can be used to understand protective mechanisms.Fat extract(FE)derived from mesenchymal stem cell therapy contains high levels of paracrine factors.AIM To study the effects of FE on myocardial injury and its mechanism of action.METHODS A mouse model of myocardial infarction and a hypoxic model of neonatal rat cardiomyocytes(CMs)were established to evaluate the effects of FE.RESULTS FE exhibited an inhibitory effect on CM apoptosis and improved left ventricular function.This protective effect of FE on CMs was mediated,in part,by the activation of the phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin signaling pathway.CONCLUSION Our findings showed that FE could be a new treatment to protect CMs in ischemic heart disease.展开更多
The lack of macro-continuity and mechanical strength of covalent organic frameworks(COFs)has significantly limited their practical applications.Here,we propose an“alcohol-triggered defect cleavage”strategy to precis...The lack of macro-continuity and mechanical strength of covalent organic frameworks(COFs)has significantly limited their practical applications.Here,we propose an“alcohol-triggered defect cleavage”strategy to precisely regulate the growth and stacking of COF grains through a moderate reversed Schiff base reaction,realizing the direct synthesis of COF nanofibers(CNFs)with high aspect ratio(L/D=103.05)and long length(>20μm).An individual CNF exhibits a biomimetic scale-like architecture,achieving superior flexibility and fatigue resistance under dynamic bending via a multiscale stress dissipation mechanism.Taking advantages of these structural features,we engineer CNF aerogels(CNF-As)with programmable porous structures(e.g.,honeycomb,lamellar,isotropic)via directional ice-template methodology.CNF-As demonstrate 100%COF content,high specific surface area(396.15 m^(2)g^(-1))and superelasticity(~0%elastic deformation after 500 compression cycles at 50%strain),outperforming most COF-based counterparts.Compared with the conventional COF aerogels,the unique structural features of CNF-A enable it to perform outstandingly in uranium extraction,with an 11.72-fold increment in adsorption capacity(920.12 mg g^(-1))and adsorption rate(89.9%),and a 2.48-fold improvement in selectivity(U/V=2.31).This study provides a direct strategy for the development of next-generation COF materials with outstanding functionality and structural robustness.展开更多
As an important class of phenanthroline derivatives containing soft N and hard O donor atoms,the laborious syntheses of unsymmetrical 1,10-phenanthroline-derived diamide ligands(DAPhen) have hindered its extensive stu...As an important class of phenanthroline derivatives containing soft N and hard O donor atoms,the laborious syntheses of unsymmetrical 1,10-phenanthroline-derived diamide ligands(DAPhen) have hindered its extensive study.In this work,we first report a convenient synthetic method for the construction of DAPhen using Friedländer reaction by two facile steps(vs.previous 12 steps).A variety of DAPhen ligands are readily available,especially unsymmetrical ones,which give us a platform to systematically study the substituent effect on f-block elements extraction performance.The performance of unsymmetrical extractants is experimentally confirmed to falls between that of their corresponding symmetrical extractants by extracting UO_(2)^(2+) as the representative f-block element.This work provides a direct and versatile method to synthesize symmetrical and unsymmetrical DAPhen,which paves way for the investigations on their coordination properties with metal ions and other applications.展开更多
文摘Moringa oleifera(MO)is traditionally used to mitigate inflammatory-mediated disorders;however,the influence of ecotypic variation on its anti-inflammatory activity remains poorly understood.In this study,we compared the phytochemical composition and anti-inflammatory activity of ethanolic extracts obtained from fresh and dried leaves of four MO ecotypes(India,Paraguay,Mozambique,and Pakistan),all grown under the same outdoor conditions,as well as two commercial powders(Just Moringa and WISSA),using LPS-stimulated RAW 264.7 macrophages.Extracts from fresh leaves were 19-43%more cytotoxic than those from dried leaves,depending on the ecotype,likely due to higher cyanogenic glycoside content.Extracts from the India and Paraguay ecotypes,characterized by high levels of quercetin derivatives and caffeic acids,as well as Just Moringa,enriched in kaempferol derivatives,significantly inhibited LPS-induced nitric oxide(NO)production(p<0.05).Just Moringa and Paraguay extracts also reduced iNOS gene expression(p<0.05 and p<0.01,respectively),whereas only the Paraguay extract decreased iNOS protein levels(p<0.05).In contrast,quercetin-3-O-glucoside and rutin showed significant effects only at concentrations approximately 100-fold higher than those present in the extracts,indicating that the phytocomplex displays greater bioactivity than individual compounds.Overall,these results demonstrate that ecotypic variation strongly affects the polyphenolic composition and anti-inflammatory properties of MO leaves,highlighting the importance of reporting both origin and phytochemical composition in MO-based products.
文摘Background: Plant-based diets are eating patterns focusing on high intake of plant-basedfoods while limiting the intake of animal-based foods. In recent decades, plant-based dietshave received increasing attention for their potential benefits for humans and theenvironment. However, a comprehensive overview of the development trends and researchhotspots of plant-based diets is still lacking. Therefore, this scientometric study aimed to fillthis critical research gap. Methods: English literature related to plant-based diets publishedfrom 2012 to 2023 in Science Citation Index Expanded (SCIE) of Web of Science CoreCollection was collected on August 16, 2023, using relevant key terms. R-bibliometrix,CiteSpace, and VOSviewer were used to analyze data and construct knowledge graphs.Results: Overall, 4135 publications (3127 articles and 1008 reviews) were included in thisstudy, showing an obvious upward trend in this field annually from 2012 to 2023, rangingfrom 70 to 921. The USA published the highest number of studies (n =1158) and was theleading research center in this field worldwide. Universities were the main researchinstitutions;particularly, Harvard T.H. Chan School of Public Health contributed to thelargest number of publications (n = 88) and had an active cooperation with otherinstitutions. Mcclements DJ contributed the most publications (n = 49) and ranked firstamong the scholars in plant-based diets. The most productive academic journal (n = 353)on plant-based diets was Nutrients, with an IF2022 of 5.9, while Foods and Frontiers in Nutritionwere the relatively active journals in this field in recent years. “Quality,” “milk,” “culturedmeat,” “plant protein,” “environmental impacts,” “functional properties,” “plant-based meatalternative,” “dietary change,” “vegan diet,” “plant-based beverage,” “gut microbiota,”“middle-income countries,” and “chronic kidney disease” were the main research hotspotsand frontiers in the field of plant-based diets. Conclusion: This study provides acomprehensive overview of global research on plant-based diets. Although research in thisfield has gained increasing attention in recent decades, cooperation among differentinstitutions and researchers should be significantly strengthened. Moreover, more studiesare needed to further assess the health and environmental impacts of plant-based diets.
基金supported by the Key Research and Development Program of Hunan Province of China(No.2022NK2036)Xiangxi Prefecture Science and Technology Plan Project"School-Local Integration"Special Project(No.2022001)the scientific research project of Hunan Provincial Department of Education(No.22B0520).
文摘Background:Ampelopsis grossedentata,vine tea,which is the tea alternative beverages in China.In vine tea processing,a large amount of broken tea is produced,which has low commercial value.Methods:This study investigates the influence of different extraction methods(room temperature water extraction,boiling water extraction,ultrasonic-assisted room temperature water extraction,and ultrasonic-assisted boiling water extraction,referred to as room temperature water extraction(RE),boiling water extraction(BE),ultrasonic assistance at room temperature water extraction(URE),and ultrasonic assistance in boiling water extraction(UBE))on the yield,dihydromyricetin(DMY)content,free amino acid composition,volatile aroma components,and antioxidant properties of vine tea extracts.Results:A notable influence of extraction temperature on the yield of vine tea extracts(P<0.05),with BE yielding the highest at 43.13±0.26%,higher than that of RE(34.29±0.81%).Ultrasound-assisted extraction significantly increased the DMY content of the extracts(P<0.05),whereas DMY content in the RE extracts was 59.94±1.70%,that of URE reached 66.14±2.78%.Analysis revealed 17 amino acids,with L-serine and aspartic acid being the most abundant in the extracts,nevertheless ultrasound-assisted extraction reduced total free amino acid content.Gas chromatography-mass spectrometry analysis demonstrated an increase in the diversity and quantity of compounds in the vine tea water extracts obtained through ultrasonic-assisted extraction.Specifically,69 and 68 volatile compounds were found in URE and UBE extracts,which were higher than the number found in RE and BE extracts.In vitro,antioxidant activity assessments revealed varying antioxidant capacities among different extraction methods,with RE exhibiting the highest DPPH scavenging rate,URE leading in ABTS•+free radical scavenging,and BE demonstrating superior ferric ion reducing antioxidant activity.Conclusion:The findings suggest that extraction methods significantly influence the chemical composition and antioxidant properties of vine tea extracts.Ultrasonic-assisted extraction proved instrumental in elevating the DMY content in vine tea extracts,thereby enriching its flavor profile while maintaining its antioxidant properties.
基金funded by Earmarked Fund for China Agriculture Research System(CARS-14)the Central Public-interest Scientific Institution Basal Research Fund(11021716001100B)。
文摘Plant-based milk is rich in polyunsaturated fatty acids,polyphenols and other bioactive compounds.This study investigated the effect of 3 plant-based milk(flaxseed milk,oat milk and soy milk)on the ceftriaxone-induced intestinal disorders,and compared the regulation patterns associated with gut microbiome and metabolome.The results showed plant-based milk alleviated the ceftriaxone caused cecum swelling,colonic tissue damage and intestinal microecological disorders.Meanwhile,administered plant-based milk decreased levels of pro-inflammatory cytokine(tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β)and oxidative stresses(malondialdehyde(MDA)and myeloperoxidase(MPO)in the colon,as well as increasing the levels of tight junction proteins(Occludin,Claudin-1,and ZO-1)in the colon.Moreover,administration of plant-based milk modulated the intestinal microbiota by promoting the relative levels of beneficial bacteria(Bifidobacterium),and inhibiting the harmful bacteria genus(Enterococcus).Furthermore,plant-based milk treatment significantly modulated glycerophospholipids metabolism(e.g.glycerophosphocholine)and arachidonic acid metabolism(e.g.prostaglandin G2 and arachidonic acid)in the serum.In conclusion,plant-based milk could alleviate antibiotic-related imbalance of barrier function damage,gut microbiota disorders and the reduction of metabolic disorders,which lays a foundation for exploring anti-inflammatory and intestinal micro-ecological approach to plant-based milk.
基金Universiti Putra Malaysia Inisiatif Putra Siswazah Grant,with a reference to UPM.RMC.800-2/1/2022/GPIPS/9740400Ministry of Higher Education,Malaysia(01-01-20-2323FR,with reference code:FRGS/1/2020/STG01/UPM/02/2)for the financial support。
文摘Plant-based milks are on the rise due to an increased awareness of their sustainability and health benefits.Currently,dairy milk is the most nutritionally complete beverage,but it suffers from the presence of indigestible lactose and allergenic proteins.Coconut milk has been around for a long time,but its application is limited due to a perceived lack of specific nutrients,high saturated fat levels,and low acceptability.Recent evidence indicates,however,that the saturated fat and other plant-based components found in coconut milk are good for metabolic outcomes and brain health.The conversion of coconut milk to yoghurt will further improve its functionality by boosting its existing nutritional qualities.In this article,the nutritional value of coconut milk,as well as its potential downsides,its application as yoghurt,and suggestions for enhancing its nutritional functionality will be examined.
基金funded by the Research Fund(Project Number 2025YB12)Innovation and Entrepreneurship Project(2024)of Shanghai Sanda University.
文摘Consumers are increasingly demanding natural colorants that are safe and offer health benefits.In addition to their ornamental characteristics,Kanzan cherry(KC)blossoms present a promising source of red-hued natural colorants and functional bioactive substances.This research utilized distilled water to extract KC petals(KCP)and their ground powders(KCPP)under varying temperatures(30℃–90℃)and times(30–180 min).The total monomeric anthocyanins(TMAC)and total phenolics(TPC)in the extracts were evaluated via the pH differential and Folin–Ciocalteu methods.Antioxidant capacities were assessed by DPPH free radical scavenging ability and reducing power.Results indicated that the optimal extraction of TMAC and TPC from KCP occurred at 90℃ for 30 min,and the resulting extracts exhibited the highest antioxidant activities among all tested temperatures and durations.Compared to different particle sizes,the finest KCPP generally produced extracts with the highest TMAC,TPC,and antioxidant activity,due to enhanced mass and heat transfer.When compared with the acidified alcohol method,hot water extraction resulted in 68.23%and 71.41%TMAC yields for petals and powders,respectively,while TPC levels were similar or higher.TMAC or TPC showed a significantly positive correlation(p<0.01)with the antioxidant activities.These findings demonstrate that hot water extraction is a viable and environmentally friendly alternative for phytochemical recovery from KC.Additionally,elevated extraction temperature and pH accelerated anthocyanin degradation and shortened its half-life,while higher pH also lowered the activation energy,enthalpy,entropy,and Gibbs free energy.Thus,red–orange KC extracts with rich bioactivity may serve as promising ingredients for functional foods having acidic pH levels.
基金The Contract Grant from Zhejiang Fengan Biopharmaceutical Co.,Ltd,Zhejiang Province,China。
文摘Traditional Chinese Medicine(TCM)has been utilized in China for thousands of years to treat and prevent various diseases,with recent research highlighting microRNAs(miRNAs)in herbal decoctions as key bioactive components.The oral lyophilized powder of the pig spleen extracts(PAT)composed of oligonucleotides and peptides extracted from fresh pig spleen and delivered via a lipid mixture containing neutral cytidinyl lipid DNCA and cationic lipid CLD(Mix),has demonstrated significant antitumor effects against A549 and BCPAP cells,promoting early and late apoptosis by approximately 20%in A549 cells.Intratumoral administration of PAT/Mix in A549-bearing mice significantly reduced both tumor volume and weight.Transcriptomic and proteomic analyses suggested that PAT/Mix exerted its antitumor activity by silencing DEPDC1 mRNA in A549 cells,thereby influencing cellular processes and metabolic pathways.Additionally,PAT/Mix was found to promote the maturation of bone marrow dendritic cells(BMDCs),enhancing antigen processing and presentation.These findings indicated that PAT/Mix held promise as a therapeutic agent with both immunomodulatory and antitumor properties,owing not only to its peptide components but also to miRNAs,which played a critical therapeutic role.
基金Supported by Putuo District Science and Technology R&D Platform Project,Shanghai(2024QX04).
文摘[Objectives]Using Calamine Zinc Oxide Phytocomplex Cream as an example,this study employed network pharmacology to investigate the therapeutic potential and mechanism of action of the combination of calamine,zinc oxide,and plant extracts in eczema intervention.[Methods]Active constituents of Calamine Zinc Oxide Phytocomplex Cream were identified through screening using the HIT2.0,HERB,and TCMSP databases.Corresponding targets of the active constituents were predicted using NetInfer.The collected targets were intersected with eczema and atopic dermatitis(AD)-related targets obtained from the GeneCards database to identify the effective therapeutic targets of Calamine Zinc Oxide Phytocomplex Cream.The network diagram of effective active constituents versus therapeutic targets for Calamine Zinc Oxide Phytocomplex Cream was constructed and subjected to topological analysis using Cytoscape software.The Protein-Protein Interaction(PPI)network was established and analyzed using the String database,Cytoscape software,and the cytoHubba plugin to identify key hub genes.Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed on the therapeutic targets using the DAVID database.[Results]Screening identified 57 active constituents in Calamine Zinc Oxide Phytocomplex Cream,corresponding to 601 potential targets.Subsequent analysis revealed 72 core therapeutic targets of Calamine Zinc Oxide Phytocomplex Cream specifically relevant to eczema and AD.Analysis of the network diagram suggested that Calamine Zinc Oxide Phytocomplex Cream may exert anti-inflammatory and immunomodulatory effects through active constituents such as quercetin,luteolin,and apigenin,while concurrently repairing skin barrier function by acting on targets including AKT1,NF-κB,and STAT3.Furthermore,the inclusion of mineral-based medicines provides additional functions such as itch relief and reinforcement of the skin barrier.[Conclusions]Calamine Zinc Oxide Phytocomplex Cream combines organic and inorganic constituents,synergistically alleviating the adverse symptoms of eczema and AD through multiple pathways.
基金supported by grants from CAAST-ACLH(NAHEP/CAAST/2018-19)of the ICAR-World Bank-funded National Agricultural Higher Education Project(NAHEP).
文摘Background:Fruits of Indian gooseberry or Amla or Aonla(Phyllanthus emblica)are important ingredients in many Ayurvedic medicines,but little is known about its leaves.Methods:Different extracts and crude alkaloids of P.emblica leaves were evaluated for their antimicrobial activity against clinically important microbes using agar well diffusion assay.The antimicrobial activity of methanolic extract(ME)of P.emblica leaves was also compared with similarly prepared methanolic extracts from leaves of Yellow Kaner(Cascabela peruviana),Parijaat or Harsingar(Nyctanthes arbor-tristis),Custard apple(Annona squamosa),Garlic vine(Mansoa alliacea),Shami plant(Prosopis cineraria),Madar(Calotropis gigantea),and Bael(Aegle marmelos).Results:The ME of leaves of P.emblica was the most potent preparation against bacteria and yeast.Of the 338 strains of microbes belonging to 100 species(96 bacteria and four Candida species),the ME of P.emblica inhibited the growth of 300 strains.A total of 84.62%,96.39%,and 100%of 221 G−bacteria,111 G+bacteria,and 6 Candida species strains,respectively,were inhibited by ME of P.emblica leaves at≤36 mg/well.The aqueous extract of P.emblica leaves also inhibited a similar number of bacterial strains,but at higher concentrations,while the ether extract could inhibit only staphylococci.The alkaloid from P.emblica leaves and the ME from leaves of other plants had insignificant antimicrobial activity at similar≤36 mg/well concentration.Conclusion:The study concluded that the ME of P.emblica leaves may be a useful source of a potent,wide-spectrum antimicrobial substance(s).
文摘BACKGROUND The rising global burden of liver diseases,such as non-alcoholic fatty liver disease and liver fibrosis,has necessitated innovative therapeutic approaches.Plant-based therapies,recognized for their anti-inflammatory and antioxidant properties,have shown promising effects.However,poor bioavailability limits their clinical application.AIM To map global research trends,key contributors,and emerging themes in plant-based therapies combined with advanced drug delivery systems for liver health.METHODS Using the Scopus database,645 documents were retrieved and analyzed using bibliometric tools Biblioshiny and VOSviewer.Analysis focused on publication trends,geographical contributions,and advancements in drug delivery technologies,including nanoparticles,liposomes,and polymeric micelles.Metrics such as publication growth rate,authorship collaboration,and thematic clustering were assessed.RESULTS The dataset spans 43 years(1981-2024),with an annual growth rate of 11.09%in the number of publications.Research output is dominated by China(33%),followed by the United States(24%)and India(18%).Collaborative studies accounted for 24.34%of publications,with an average of 5.81 co-authors per document.Key innovations include nanoparticle encapsulation of curcumin and silymarin,improving bioavailability by up to 85%.Highly cited studies demonstrated the antioxidant,anti-inflammatory,and anti-fibrotic properties of these compounds.For instance,curcumin nanoparticles showed a 70%improvement in solubility,and silymarin liposomal formulations enhanced therapeutic efficiency by 62%.Thematic analysis revealed a transition from basic clinical observations to molecular and pharmacokinetic research,with a focus on oxidative stress mitigation and hepatoprotection.CONCLUSION This study highlights the growing synergy between plant-based therapies and advanced drug delivery systems,with significant contributions from Asian and Western countries.Future efforts should prioritize clinical trials,standardization of plant extract formulations,and interdisciplinary approaches to maximize therapeutic outcomes.The findings provide a foundation for integrating plant-derived compounds into evidence-based hepatological therapies,addressing critical challenges in bioavailability and safety.
基金supported by the Estonian Research Council(ETAG)[grants number PRG1808 and PRG1223].
文摘Gilia capitata Sims(Polemoniaceae)is an annual herbaceous plant widely distributed in western North America and cultivated as an ornamental flower.The detailed chemical composition and pharmacological properties of this plant have not been previously reported.This study aimed to investigate the chemical composition and pharmacological activity of aqueous-ethanolic extracts derived from different plant parts(herb,flowers,stems,roots,and fruits).HPLC analysis identified 12 phenolic compounds and 16 amino acids in the aqueous-ethanolic extracts obtained from these plant parts.The biological effects of the extracts were evaluated in vitro,including cytotoxicity,anti-inflammatory response,andwound-healing potential.The anti-inflammatory activity was evaluated by measuring their effect on NO production in LPS-stimulated RAW264.7 murine macrophages and HaCaT keratinocytes.Phagocytic activity was assessed using the neutral red uptake assay.HaCaT cell proliferation and migration were assessed to evaluate the wound-healing potential.Morphological changes in both cell lines were examined microscopically.Moreover,neurobehavioral effects were studied in vivo.The herb extracts significantly enhanced locomotor activity while preserving exploratory behaviour,suggesting a stimulating effect.G.capitata flower extracts caused a decrease in both locomotor and exploratory behaviour,demonstrating a moderate sedative effect.Fruit extracts significantly suppressed locomotor and exploratory activity,indicating a strong sedative effect.These findings indicated that G.capitata contains bioactive compounds with diverse pharmaceutical effects depending on the specific plant part and may have potential applications in medicine and pharmacology pending further research.
文摘Anoectochilus roxburghii from different planting sources was used as the research object,and the biological activity of its extracts was compared.The results showed that the whole plant extracts of A.roxburghii planted in bag culture (BAG),greenhouse cultivation (GRE) and tissue culture seedlings (TIS) were rich in active ingredients such as A.roxburghii polysaccharides and A.roxburghii glycosides.In vitro experiments showed that Anoectochilus roxburghii extracts from different planting sources had good antioxidant capacity,tyrosinase inhibition ability and hyaluronidase inhibition ability.Among them,the GRE Anoectochilus roxburghii whole plant extract had the strongest scavenging ability on DPPH free radical,ABTS free radical and hydroxyl free radical,and the inhibitory effect on tyrosinase activity and hyaluronidase activity was significantly higher than that of BAG and TIS.At the same time,it also verified that the extract of Anoectochilus roxburghii has antioxidant,whitening,anti-inflammatory and other skin care effects,which provides a theoretical basis for the development and application of Anoectochilus roxburghii extract in the field of cosmetics.
基金PROTEXTWOOD (ID 2202-102) funded through LabEx AGRO ANR-10-LABX-0001-01 (under ISiteUniversité de Montpellier framework)the project PANTHER2-Guyane funded through AgenceNationale de la Recherche (ANR-22-CE43-0019)+2 种基金“Investissement d’Avenir” grant managed by Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-25-01)supported by the FEDER (European Regional Development Fund)research project “EcovaloBois” (Project number: GY0015430)by the CNRS peps INSIS2018 research project “GuyavaloFibres”.
文摘The valorization of Amazonian wood residues into active chemical compounds could be an eco-friendly,cost-effective and valuable way to develop wood preservative formulations to enhance the decay and termite resistance of low-durable wood species.Wacapou(Vouacapoua americana.,Fabaceae)is a well-known Guianese wood spe-cies commonly used in local wood construction due to its outstanding natural durability,which results from the presence of a large panel of extractives compounds.In addition,its industrial processing generates large amounts of residues.Wacapou residues were extracted by maceration using four different solvents(water/ethanol,ethyl acetate,hexane and dichloromethane/methanol),separately and successively.The yield of each extractive fraction was determined,and their chemical compositions were analyzed by Liquid Chromatography-Mass Spectrometry(LC-MS).Ethyl acetate led to the highest extraction yield,and the active compounds were identified in the obtained extractive fraction.In this sense,the fungicidal and termite-repellent properties of these extractives were then tested using a screening laboratory(with temperate and tropical microorganisms),according to the solution concentration(1%,2.5%,5%,8%and 10%).Finally,Virola michelii Heckel wood samples(low durable species)were impregnated with the 8%concentration solution.The impregnated wood samples were then exposed to a soil bed test.The results highlighted that the nature of the solvent used during wood maceration affects the con-tent of the obtained extractive fractions.Ultra-Performance Liquid Chromatography–High-Resolution Mass Spectrometry(UHPLC-HRMS)analyses showed the influence of extraction parameters on the nature of the extracted molecules.Wacapou extracts(from ethyl acetate maceration)showed good anti-fungal and anti-termite activities.Additionally,the concentration in extractives had an impact on the anti-termite activity level for Reti-culitermesflavipes and Cryptotermes sp.Formulations based on Wacapou extractives showed a good potential for valorization in eco-friendly preservatives,aiming to confer better durability to local low-durability wood species.
基金supported in part by the Natural Science and Technology Foundation of Guizhou Province(QiankeheJichu-ZK[2022]Yiban606)National Natural Science Foundation of China(82160181)+1 种基金Science and Technology Foundation of Guizhou Health and Health Committee(gzwkj2024-455)Zunyi Oral Disease Immune Prevention and Medical Biomaterials Research and Development Innovation Talent Team(Zunyi Science Talent[2022]No.1).
文摘Periodontitis is an inflammatory infectious disease affecting the periodontal supporting tissues and is the primary cause of tooth loosening and tooth loss in adults.Clinically,supragingival scaling,subgingival scaling,root planing,and other basic periodontal treatments,often combined with antibiotic therapy,are commonly employed with moderate efficacy.However,the increasing prevalence of antibiotic resistance and associated adverse reactions has become a growing concern.Recent studies have demonstrated the significant impact of traditional Chinese medicine(TCM)extracts in both the prevention and treatment of periodontitis,exhibiting remarkable effectiveness.This review explores the role and mechanisms of TCM extracts in the prevention and treatment of periodontitis,providing a reference for further elucidation of their mechanisms and a theoretical basis for the development of Chinese herbal medicine-based care products.
文摘Standard bacterial suspensions play a crucial role in microbiological diagnosis.Traditional prepar-ation methods,which rely heavily on manual operations,face challenges such as poor reproducibility,low ef-ficiency,and biosafety concerns.In this study,we propose a high-precision automated colony extraction and separation system that combines large-field imaging and artificial intelligence(AI)to facilitate intelligent screening and localization of colonies.Firstly,a large-field imaging system was developed to capture high-resolution images of 90 mm Petri dishes,achieving a physical resolution of 13.2μm and an imaging speed of 13 frames per second.Subsequently,AI technology was employed for the automatic recognition and localiza-tion of colonies,enabling the selection of target colonies with diameters ranging from 1.9 to 2.3 mm.Next,a three-axis motion control platform was designed,accompanied by a path planning algorithm for the efficient extraction of colonies.An electronic pipette was employed for accurate colony collection.Additionally,a bacterial suspension concentration measurement module was developed,incorporating a 650 nm laser diode as the light source,achieving a measurement accuracy of 0.01 McFarland concentration(MCF).Finally,the system’s performance was validated through the preparation of an Esckerichia coli(E.coli)suspension.After 17 hours of cultivation,E.coli was extracted four times,achieving the target concentration set by the system.This work is expected to enable rapid and accurate microbial sample preparation,significantly reducing de-tection cycles and alleviating the workload of healthcare personnel.
基金financially supported by the Elite Researcher Grant Committee(No.971375)from the National Institutes for Medical Research Development(NIMAD),Tehran,Iran。
文摘The goal of this study was to synthesize nickel oxide nanoparticles(NiO-NPs)by the sol-gel method,which involved the use of salvia macrosiphon Boiss plant extract,Ni(NO3)2·6 H2 O as a capping agent,and a nickel precursor,respectively.The synthesized NiO-NPs were characterized by Fourier transform infrared spectroscopy(FTIR),ultraviolet-visible(UV-Vis)spectrophotometer,X-ray diffraction(XRD),field electron scanning electron microscopy(FESEM)/energy-dispersive X-ray spectroscopy(EDX),thermo-gravimetric analysis/differential thermal analysis(TGA/DTA)and vibrating sample magnetometer(VSM)analyzes.Also,according to the results of UV-Vis,the gap band of nanoparticles was calculated to be in the range of about 2.9-3.9 eV.The photocatalytic activity of nanoparticles on methylene blue(MB)degradation was investigated and according to the results,about 80%MB was apparently degraded in the presence of NiO-NPs under UV-A light(11 W)after 5 h in pH^11.We have evaluated the cytotoxicity of NiO-NPs on the multiple tumor cells by materials method,and all the utilized concentrations were observed to cause non-toxic effects;therefore,it can be suggested that these samples have the potential of being employed in different fields of medicine.
基金the financial support from the National Natural Science Foundation of China(22325603)the financial support from the National Natural Science Foundation of China(22376116)+3 种基金the financial support from the National Natural Science Foundation of China(22076130)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(CAST)(2023QNRC001)the Fundamental Research Funds for the Central Universities(20826041D4117)the Natural Science Foundation of Sichuan(2025ZNSFSC0109)。
文摘The recycling of neptunium(Np)from nuclear wastes is crucial for the sustainable development of nuclear energy,yet it is still a challenging task owing to the complexity of Np chemistry.Precise control of oxidation state is highly desirable for the effective recovery of Np.In this study,we report an innovative strategy for Np recovery through in-situ coordination and reduction of Np(Ⅴ)in a biphasic extraction system.By leveraging the synergistic effects of coordination by a P=O donating ligand(trialkyl phosphine oxide,TRPO)and reduction by hydroquinone(HQ)in the organic phase,efficient Np(Ⅴ)-to-Np(Ⅳ)conversion and high distribution ratio(D)of Np were achieved in a single extraction contact.The reduction mechanism of Np was elucidated through spectroscopic and theoretical analyses.This work enriches the redox chemistry of Np and provides a novel pathway for Np recovery in advanced nuclear fuel cycles.
基金Supported by National Natural Science Foundation of China,No.82200270and the Vascular Disease Discipline Cluster,No.SKQJS202402.
文摘BACKGROUND Ischemic heart disease ranks among the foremost contributors to mortality worldwide.Myocardial infarction injury poses a prevalent challenge in current therapies.Studies have shown that mesenchymal stem cell transplantation increases cytokine release,reduces myocardial cell necrosis,and improves left ventricular function;thus,it can be used to understand protective mechanisms.Fat extract(FE)derived from mesenchymal stem cell therapy contains high levels of paracrine factors.AIM To study the effects of FE on myocardial injury and its mechanism of action.METHODS A mouse model of myocardial infarction and a hypoxic model of neonatal rat cardiomyocytes(CMs)were established to evaluate the effects of FE.RESULTS FE exhibited an inhibitory effect on CM apoptosis and improved left ventricular function.This protective effect of FE on CMs was mediated,in part,by the activation of the phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin signaling pathway.CONCLUSION Our findings showed that FE could be a new treatment to protect CMs in ischemic heart disease.
基金supported by the National Natural Science Foundation of China(No.52403035)the Shanghai Sailing Program(23YF1400300)+1 种基金the Fundamental Research Funds for the Central Universities(2232023D-05)the Weiqiao Teaching and Research Innovation Program.
文摘The lack of macro-continuity and mechanical strength of covalent organic frameworks(COFs)has significantly limited their practical applications.Here,we propose an“alcohol-triggered defect cleavage”strategy to precisely regulate the growth and stacking of COF grains through a moderate reversed Schiff base reaction,realizing the direct synthesis of COF nanofibers(CNFs)with high aspect ratio(L/D=103.05)and long length(>20μm).An individual CNF exhibits a biomimetic scale-like architecture,achieving superior flexibility and fatigue resistance under dynamic bending via a multiscale stress dissipation mechanism.Taking advantages of these structural features,we engineer CNF aerogels(CNF-As)with programmable porous structures(e.g.,honeycomb,lamellar,isotropic)via directional ice-template methodology.CNF-As demonstrate 100%COF content,high specific surface area(396.15 m^(2)g^(-1))and superelasticity(~0%elastic deformation after 500 compression cycles at 50%strain),outperforming most COF-based counterparts.Compared with the conventional COF aerogels,the unique structural features of CNF-A enable it to perform outstandingly in uranium extraction,with an 11.72-fold increment in adsorption capacity(920.12 mg g^(-1))and adsorption rate(89.9%),and a 2.48-fold improvement in selectivity(U/V=2.31).This study provides a direct strategy for the development of next-generation COF materials with outstanding functionality and structural robustness.
基金financial support from the National Natural Science Foundation of China (Nos.22476178,U2067213)Natural Science Foundation of Zhejiang Province (No.LRG25B060002)。
文摘As an important class of phenanthroline derivatives containing soft N and hard O donor atoms,the laborious syntheses of unsymmetrical 1,10-phenanthroline-derived diamide ligands(DAPhen) have hindered its extensive study.In this work,we first report a convenient synthetic method for the construction of DAPhen using Friedländer reaction by two facile steps(vs.previous 12 steps).A variety of DAPhen ligands are readily available,especially unsymmetrical ones,which give us a platform to systematically study the substituent effect on f-block elements extraction performance.The performance of unsymmetrical extractants is experimentally confirmed to falls between that of their corresponding symmetrical extractants by extracting UO_(2)^(2+) as the representative f-block element.This work provides a direct and versatile method to synthesize symmetrical and unsymmetrical DAPhen,which paves way for the investigations on their coordination properties with metal ions and other applications.
