This study,integrating both domestic and international literatures as well as field research findings,presents a comprehensive review of the geographical distribution,ecological and physiological characteristics of Ac...This study,integrating both domestic and international literatures as well as field research findings,presents a comprehensive review of the geographical distribution,ecological and physiological characteristics of Acanthus ebracteatus.It further delves into the factors leading to its endangered status and proposes corresponding protection strategies.The mian reasons for the endangered of A.ebracteatus are as follows:illegal harvesting of whole plants,habitat destruction,reproductive obstacles,and competition or interference from invasive or noxious plant species.To address these threats,a series of conservation strategies are proposed.Firstly,in-depth research on the artificial propagation techniques of A.ebracteatus should be conducted to surmount reproductive barriers.Secondly,habitat restoration initiatives need to be carried out to create a suitable ecological setting.Thirdly,in-situ protections should be enhanced while the implementing ex-situ conservation measures.Meanwhile,continuous monitoring of A.ebracteatus seedlings should be conducted to strengthen subsequent management and conservation efforts.Finally,it is recommended to rationally explore and utilize the medicinal properties of A.ebracteatus.展开更多
Auxin is a phytohormone that is critical for plant growth and development.The molecular mechanisms underlying auxin biosynthesis,transport,and signaling are well understood.However,the complex mechanism by which auxin...Auxin is a phytohormone that is critical for plant growth and development.The molecular mechanisms underlying auxin biosynthesis,transport,and signaling are well understood.However,the complex mechanism by which auxin regulates plant volatile biosynthesis has seldom been studied.A growing array of unique auxin-related plant volatiles have recently been discovered.This study comprehensively reviews recent findings on auxin and auxin-related genes and their roles in the formation of plant volatiles.This study highlights the implications of exogenous auxin application,genes involved in auxin signaling transduction,and hormonal crosstalk during volatile compound biosynthesis in plants.Plant hormones facilitate the integration of multiple volatile signals to enable specific and appropriate responses to environmental changes.This will improve our overall understanding of the role of auxins in plant volatile compound metabolic pathways.Recent studies have delineated the considerable advancements in elucidating the intricate methods by which plants employ auxin regulatory pathways to modulate the release of volatile chemicals during development and growth,along with prospective research paths.展开更多
N6-methyladenosine(m^(6)A)modification,the most abundant internal modification in messenger RNA(mRNA)and long non-coding RNA(lncRNA),has emerged as a critical epitranscriptomic regulatory mechanism in eukaryotes.While...N6-methyladenosine(m^(6)A)modification,the most abundant internal modification in messenger RNA(mRNA)and long non-coding RNA(lncRNA),has emerged as a critical epitranscriptomic regulatory mechanism in eukaryotes.While the importance of m^(6)A modification in various biological processes has been recognized,a comprehensive understanding of its diverse roles in plant biology and agricultural applications remains fragmented.This review analyzes recent advances inm^(6)A modification's biological functions in plants.m^(6)A modification plays crucial roles in multiple aspects of plant life,including seed germination,organ development,and reproductive structure formation.Furthermore,m^(6)A has been found to significantly influence plant responses to environmental stresses,including salt,drought,temperature,and heavy metal exposure.We also uncover m^(6)A involvement in important agricultural traits.This review provides insights into the mechanistic understanding of m^(6)A modification in plants and highlights its applications in agricultural improvement,offering a foundation for future research in crop enhancement and stress resistance.展开更多
For decades,Xu has been committed to fulfilling the duty and mission of a scientist and educator—diving into the laws of nature,caring deeply for the nation,and earnestly cultivating younger generations.
Atrazine,a persistent triazine herbicide,poses environmental and health risks.This study examines the synergis-tic remediation of atrazine-contaminated soil using green manure plant(GMP)hairy vetch(Vicia villosa Roth,...Atrazine,a persistent triazine herbicide,poses environmental and health risks.This study examines the synergis-tic remediation of atrazine-contaminated soil using green manure plant(GMP)hairy vetch(Vicia villosa Roth,VV)and the exogenous atrazine-degrading bacterium Arthrobacter sp.ATR1.Soil samples contaminated with atrazine at 5 and 20 mg/kg were treated with control(CK),ATR1(CKatr),hairy vetch(VV),and combined hairy vetch and ATR1 remediation(VVatr).The results indicated that the VVatr treatment exhibited the most effective atrazine removal,achieving enhancements of 56.12%at 5 mg/kg and 54.51%at 20 mg/kg compared to CK after 28 days.Soil enzyme activities,including urease,sucrase,and alkaline phosphatase,were significantly elevated in the VV and VVatr treatments,contributing to improved soil quality.Additionally,the CKatr,VV,and VVatr treat-ments enhanced bacterial diversity and richness while altering the microbial community structure.The VV and VVatr treatments notably enriched indigenous atrazine-degrading bacteria and nitrogen-fixing bacteria in the rhizosphere.This microbial enrichment upregulated the Atrazine degradation and Nitrogen metabolism pathways,facilitating both atrazine removal and nitrogen cycling in the soil.And VVatr treatment promoted the stability of the microbial network and enhanced the cooperative relationship between key indigenous atrazine-degrading and nitrogen-fixing bacteria.These findings explain the mechanism of plantmicrobe combined remediation of atrazine-contaminated soil from the perspective of rhizosphere microorganisms and offer a theoretical basis for the practical application of this method.展开更多
Tajikistan represents a core region of the biodiversity hotspot in Central Asian mountains and has exceptional vascular plant diversity.However,the species diversity of the country faces urgent conservation challenges...Tajikistan represents a core region of the biodiversity hotspot in Central Asian mountains and has exceptional vascular plant diversity.However,the species diversity of the country faces urgent conservation challenges.There has been a lack of a comprehensive and multidimensional assessment to inform strategic conservation planning.Therefore,this study integrated 4 key biodiversity indices including species richness(SR),phylogenetic diversity(PD),threatened species richness(TSR),and endemic species richness(ESR)to map species diversity distribution patterns,identify conservation gaps,and elucidate their effects of climatic factors.This study revealed that species diversity shows a clear trend of decreasing from the western region to the eastern region of Tajikistan.The central–western mountains(specifically the Gissar-Darvasian and Zeravshanian regions)emerge as irreplaceable biodiversity hotspots.However,we found a severe spatial mismatch between these priority areas and the existing protected areas(PAs).Protection coverage for all hotspots was alarmingly low,ranging from 31.00%to 38.00%.Consequently,a critical 64.80%of integrated priority areas fall outside of the current PAs,representing a major conservation gap.This study identified precipitation seasonality and isothermality as the principal drivers,collectively explaining over 50.00%of the diversity variation and suggesting high vulnerability to hydrological shifts.Furthermore,we detected significant geographic sampling bias in the public biodiversity databases,with the most critical hotspot being systematically under-sampled.This study provides a robust scientific basis for conservation action,highlighting the urgent need to strategically expand PAs in the under-protected southwestern region and to mitigate critical sampling gaps through targeted data digitization and field surveys.These measures are indispensable for securing Tajikistan’s unique biodiversity and achieving the Kunming-Montreal Global Biodiversity Framework Target 3(“30×30 Protection”).展开更多
Livestock farming is a critical pillar of Tajikistan’s national economy and livelihood security.However,significant economic challenges in the country have led to the degradation of grassland ecosystems.This degradat...Livestock farming is a critical pillar of Tajikistan’s national economy and livelihood security.However,significant economic challenges in the country have led to the degradation of grassland ecosystems.This degradation has not only reduced the productivity of grassland ecosystems but also severely impacted their ecological functions.A particularly concerning consequence is the threat to biodiversity,as the survival and persistence of endemic,rare,and endangered plant species are at serious risk,thereby diminishing the value of species’genetic resources.Based on the data from multiple sources such as literature reviews,field observations,and national statistics,this study employed a systematic literature review and meta-analysis to investigate the current status,causes of degradation,and restoration measures for grassland ecosystems in Tajikistan.The results revealed that Tajikistan’s grassland ecosystems support exceptionally high plant species diversity,comprising over 4500 vascular plant species,including nearly 1500 endemic and sub-endemic taxa that constitute a unique genetic reservoir.These ecosystems are experiencing severe degradation,characterized by significantly reduced vegetation cover and declining species richness.Palatable forage species are increasingly being displaced by unpalatable,thorny,and poisonous species.The primary drivers of degradation include excessive grazing pressure,which disrupts plant reproductive cycles and regeneration capacity,habitat fragmentation due to urbanization and infrastructure development,and uncontrolled exploitation of medicinal and edible plants.Climate change,particularly rising temperatures and altered precipitation patterns,further exacerbates these anthropogenic pressures.Ecological restoration experiments suggested that both ecosystem productivity and plant species diversity are significantly enhanced by systematic reseeding trials using altitude-adapted native species.These findings underscore the necessity of establishing scientifically grounded approaches for ecological restoration.展开更多
[Objectives]To investigate the effects of different planting densities and nitrogen application rates on the yield and quality of the tobacco cultivar Chuxue 80.[Methods]A field experiment was conducted in Hubei Provi...[Objectives]To investigate the effects of different planting densities and nitrogen application rates on the yield and quality of the tobacco cultivar Chuxue 80.[Methods]A field experiment was conducted in Hubei Province,evaluating various combinations of planting density and nitrogen rate for Chuxue 80.[Results]At the maturity stage,the TN1 treatment(5 kg N per 667 m^(2) with a density of 1900 plants per 667 m^(2))demonstrated the most favorable agronomic performance.The TN9 treatment(11 kg N per 667 m^(2) with a density of 1110 plants per 667 m^(2))achieved the highest wrapper tobacco yield and output value.Meanwhile,the TN5 treatment(8 kg N per 667 m^(2) with a density of 1515 plants per 667 m^(2))resulted in the best smoking quality.[Conclusions]The TN9 treatment,with a planting density of 1110 plants per 667 m^(2) and a nitrogen application rate of 11 kg per 667 m^(2),is recommended as the optimal cultivation practice for Chuxue 80 in Hubei Province.展开更多
Toxic heavy metal and metalloid(THMM)contamination poses a major global challenge,threatening human health and sustainable agriculture.The crucial role of the Cytochrome P450(CYP)gene family in plant tolerance to THMM...Toxic heavy metal and metalloid(THMM)contamination poses a major global challenge,threatening human health and sustainable agriculture.The crucial role of the Cytochrome P450(CYP)gene family in plant tolerance to THMMs has been recently highlighted,but there is still a lack of comprehensive understanding,especially in relation to metabolites.This study delved into the identification of CYP genes that are linked to the tolerance mechanisms of plants in response to heavy metal stress.The findings highlight the significant metabolic pathways that contribute to this resilience,using rice and Arabidopsis as exemplars.THMM exposure changed CYP gene expression in plants,and THMM antidotes mitigated its downregulation and that of flavonoid biosynthesis genes.CYP genes involved in THMM responses were predominantly enriched in the pathways associated with flavonoid synthesis,indicating functional adaptations to distinct stresses.Notably,anthocyanin(Ant)accumulation,a type of flavonoid,affected the uptake of various heavy metals in Brassica rapa,with flavonoid biosynthesis-associated genes correlating with Cd or As tolerance and Ant content.These findings highlight the critical importance of flavonoid metabolism and the intricate network of biosynthesis genes in bolstering plant resilience against heavy metal stress.This enhanced understanding paves the way for significant advancements in phytoremediation technologies,offering innovative solutions for soil and water decontamination.展开更多
Reactive oxygen species(ROS)act as early messengers in plants exposed to drought,salinity,heat and other environmental challenges.Their timely removal is crucial.Unchecked ROS injure membranes,macromolecules and photo...Reactive oxygen species(ROS)act as early messengers in plants exposed to drought,salinity,heat and other environmental challenges.Their timely removal is crucial.Unchecked ROS injure membranes,macromolecules and photosynthetic systems,ultimately curbing growth or causing cell death.While mitochondria possess inhouse antioxidant machinery,how non-mitochondrial systems contribute to mitochondrial redox homeostasis has remained unresolved.Laura F.DiGiovanni et al.demonstrate that peroxisomes directly protect mitochondria through contact-mediated ROS shuttling.This discovery extends the concept of organelle crosstalk beyond metabolic exchange to contact-mediated ROS flux,adding a system-level buffer against oxidative stress.Deep understanding and regulation of this pathway are highly significant for exploring how ROS coordinate plant stress responses,enhancing crop stress resistance and reducing extreme environment-induced oxidative damage.This may provide breeders and agronomists with a novel approach to develop stress-resistant traits.展开更多
SalicS1 is a genetically encoded,ratiometric FRET biosensor that brings salicylic acid(SA)research to the same real-time imaging standard long available for ABA and GA.Built through a modular Golden Gate platform and ...SalicS1 is a genetically encoded,ratiometric FRET biosensor that brings salicylic acid(SA)research to the same real-time imaging standard long available for ABA and GA.Built through a modular Golden Gate platform and informed by NPR-NIMIN structural biology,SalicS1 achieves SA specificity,tunable affinity,reversibility,and non-perturbing expression in Arabidopsis.Using this sensor,pathogen infection,non-adapted fungal challenge,and aphid feeding are shown to elicit spatially propagating SA surges rather than purely local accumulation,revealing a tissue-level organization of immune signaling that bulk assays could not resolve.SalicS1 therefore provides a broadly deployable tool for dissecting the geometry,timing,and genotype dependence of SA-mediated plant defense.展开更多
Virtual power plant(VPP)integrates a variety of distributed renewable energy and energy storage to participate in electricity market transactions,promote the consumption of renewable energy,and improve economic effici...Virtual power plant(VPP)integrates a variety of distributed renewable energy and energy storage to participate in electricity market transactions,promote the consumption of renewable energy,and improve economic efficiency.In this paper,aiming at the uncertainty of distributed wind power and photovoltaic output,considering the coupling relationship between power,carbon trading,and green cardmarket,the optimal operationmodel and bidding scheme of VPP in spot market,carbon trading market,and green card market are established.On this basis,through the Shapley value and independent risk contribution theory in cooperative game theory,the quantitative analysis of the total income and risk contribution of various distributed resources in the virtual power plant is realized.Moreover,the scheduling strategies of virtual power plants under different risk preferences are systematically compared,and the feasibility and accuracy of the combination of Shapley value and independent risk contribution theory in ensuring fair income distribution and reasonable risk assessment are emphasized.A comprehensive solution for virtual power plants in the multi-market environment is constructed,which integrates operation strategy,income distribution mechanism,and risk control system into a unified analysis framework.Through the simulation of multi-scenario examples,the CPLEXsolver inMATLAB software is used to optimize themodel.The proposed joint optimization scheme can increase the profit of VPP participating in carbon trading and green certificate market by 29%.The total revenue of distributed resources managed by VPP is 9%higher than that of individual participation.展开更多
Arid and semi-arid ecosystems are prone to extensive fires due to specific climatic conditions,sparse vegetation cover,and high density of fine fuels.Understanding the flammability characteristics of land covers is es...Arid and semi-arid ecosystems are prone to extensive fires due to specific climatic conditions,sparse vegetation cover,and high density of fine fuels.Understanding the flammability characteristics of land covers is essential for fire management and designing land restoration programs in arid and semi-arid ecosystems.This study provided a new approach to evaluate the flammability of shrublands and woodlands using flammability indices(FIs)including time to ignition(TI),duration of combustion(DC),and flame height(FH)of plant species and their relative frequencies in the Dalfard Basin of southeastern Iran.The results showed that there was a significant difference in FIs between land covers.Shrublands had higher flammability potential compared with woodlands.Plant moisture content had a negative relationship with TI(P<0.010)and no significant relationship with DC and FH(P>0.050).Artemisia spp.,Astragalus gossypinus Fischer,Amygdalus scoparia Spach,and Cymbopogon jwarancusa(Jones)Schult.had the highest FI.Tree species such as Rhazya stricta Decne.,and Pistacia atlantica Desf.showed greater resistance to fire.Using principal component analysis,the relationship between species and FIs was examined,and TI of wet fuel was the most important FI in relation to species.Structural equation model showed that life form(P<0.001)was the most important flammability driver.Precipitation(P<0.010)and legume species(P<0.010)were significantly related to the flammability in arid land.This study emphasizes the importance of managing high-risk species and using resistant species in vegetation restoration and shows that combining species FIs with their abundance is an effective tool for assessing fire risk and fuel management at the plant community scale.展开更多
This paper suggests a way to improve teamwork and reduce uncertainties in operations by using a game theory approach involving multiple virtual power plants(VPP).A generalized credibility-based fuzzy chance constraint...This paper suggests a way to improve teamwork and reduce uncertainties in operations by using a game theory approach involving multiple virtual power plants(VPP).A generalized credibility-based fuzzy chance constraint programming approach is adopted to address uncertainties stemming from renewable generation and load demand within individual VPPs,while robust optimization techniques manage electricity and thermal price volatilities.Building upon this foundation,a hierarchical Nash-Stackelberg game model is established across multiple VPPs.Within each VPP,a Stackelberg game resolves the strategic interaction between the operator and photovoltaic prosumers(PVP).Among VPPs,a cooperative Nash bargaining model coordinates alliance formation.The problem is decomposed into two subproblems:maximizing coalitional benefits,and allocating cooperative surpluses via payment bargaining,solved distributively using the alternating direction method of multipliers(ADMM).Case studies demonstrate that the proposed strategy significantly enhances the economic efficiency and uncertainty resilience of multi-VPP alliances.展开更多
Rapidly improving infertile croplands and enhancing their soil organic carbon(SOC)pool necessitate substantial organic materials incorporation.Converting loose crop straw into granulated form facilitates uniform incor...Rapidly improving infertile croplands and enhancing their soil organic carbon(SOC)pool necessitate substantial organic materials incorporation.Converting loose crop straw into granulated form facilitates uniform incorporation within the plough soil layer.As an innovative soil amelioration approach,the efficiency and patterns of SOC accumulation remain unclear.Two field experiments were conducted in infertile subtropical upland and paddy soils with 0,30,60,and 90 Mg ha^(-1)granulated straw incorporation.After one year,SOC accumulation efficiency from straw input remained stable in upland(30.8–37.5%)with increasing amounts of straw incorporation,while declined from 60.0 to 38.3%in paddy.In both croplands,the contributions of lignin phenols to SOC increased with increasing straw incorporation,while the contributions from amino sugars remained constant at higher straw input levels.Subsequently,the ratios of lignin phenols to amino sugars increased with increasing straw incorporation,indicating faster plant residue accumulation compared to microbial necromass,as the granulation approach limited microbial involvement in straw transformation.Thus,single-time incorporation of substantial granulated straw presents an effective agricultural strategy for rapid amelioration of infertile croplands.展开更多
Triacylglycerol(TAG)components in human milk,infant formulas with different fat sources,and plant oils(palm oil,flaxseed oil,sunflower oil,corn oil,soybean oil,coconut oil,low erucic acid rapeseed oil,and high oleic a...Triacylglycerol(TAG)components in human milk,infant formulas with different fat sources,and plant oils(palm oil,flaxseed oil,sunflower oil,corn oil,soybean oil,coconut oil,low erucic acid rapeseed oil,and high oleic acid rapeseed oil)were analyzed and compared using ultraperformance supercritical fluid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPSFC-Q-TOF-MS).Distinct TAG profiles were identified by lipidomics,principal component analysis(PCA),and heatmap visualization.Human milk was characterized by a high abundance of medium-and long-chain triacylglycerols(MLCTs),saturated-unsaturated-unsaturated(SUU)-type TAGs(sn-2 palmitic acid).In contrast,plant oils lacked MLCTs and SUU-type TAGs,containing mainly TAGs esterified long-chain polyunsaturated fatty acid.Breast milk contains about 27%MLCT,about 50%SUU-type triacylglycerols(which contain about 34%UPU-type triacylglycerols),which is significantly different from plant oils,structured fats and infant formulas.These findings provide a clear basis for optimizing the fat blend in infant formula to better mimic the unique TAG profile of human milk,thereby improving energy delivery and nutrient absorption for infants.展开更多
Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved i...Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement.WRKY transcription factors regulate various plant processes,including growth and development,stress responses,and metabolite biosynthesis.However,the molecular network through which WRKY coordinates these pathways in tea plants remains unclear.In this study,we revealed that CsWRKY57L,a cold-inducible WRKY IIc subfamily member,positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants.Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor(CBF)-cold-responsive(COR)gene pathway.In contrast,heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect.Additionally,overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis.Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a,CsSWEET15,and AtSWEET15,which encode sugar transporters essential for plant reproductive development,and inhibits their transcription.Overall,the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression.This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants,providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.展开更多
The main purpose of using geothermal energy piles(GEPs)is to enable the exploitation of geothermal energy for meeting the heating/cooling demands of buildings efficiently.However,the installation process of convention...The main purpose of using geothermal energy piles(GEPs)is to enable the exploitation of geothermal energy for meeting the heating/cooling demands of buildings efficiently.However,the installation process of conventional GEPs is inconvenient compared with that of traditional foundation piles.The pre-bored grouted planted geothermal energy pile(PGP GEP)is an innovative technology to simplify the installation process.Most investigations of in-situ experiments for conventional GEPs have focused on summer conditions.An in-situ test for a PGP GEP was conducted to analyze the temperature changes and thermo-mechanical characteristics under winter conditions.The results show that the average temperature of the pile decreased by 5.1℃,and the pile exhibited a general trend of high temperatures at both ends and low temperatures in the middle.In mechanics,strong pile end restraints resulted in smaller observed axial strain and higher axial thermal-induced force in the pile ends than at the middle of the pile.展开更多
基金Supported by Special Innovation Projects for Regular Institutions of Higher Education in Guangdong Province(2023KTSCX071)Zhanjiang Science and Technology Bureau Project(2023A01018)Colonel-level Project(LY2207).
文摘This study,integrating both domestic and international literatures as well as field research findings,presents a comprehensive review of the geographical distribution,ecological and physiological characteristics of Acanthus ebracteatus.It further delves into the factors leading to its endangered status and proposes corresponding protection strategies.The mian reasons for the endangered of A.ebracteatus are as follows:illegal harvesting of whole plants,habitat destruction,reproductive obstacles,and competition or interference from invasive or noxious plant species.To address these threats,a series of conservation strategies are proposed.Firstly,in-depth research on the artificial propagation techniques of A.ebracteatus should be conducted to surmount reproductive barriers.Secondly,habitat restoration initiatives need to be carried out to create a suitable ecological setting.Thirdly,in-situ protections should be enhanced while the implementing ex-situ conservation measures.Meanwhile,continuous monitoring of A.ebracteatus seedlings should be conducted to strengthen subsequent management and conservation efforts.Finally,it is recommended to rationally explore and utilize the medicinal properties of A.ebracteatus.
基金supported by the National Natural Science Foundation of China(Grant Nos.W2433051,32272663,32302520,and 32060655)National Key Research and Development Program(Grant No.2023YFD2300800)+3 种基金the Guangzhou Science and Technology Project(Grant No.202206010023)Yunnan Provincial Science and Technology Department Local Universities Joint Project(Grant No.202101BA070001-166)China Litchi and Longan Industry Technology Research System(CARS-32-08),Talent Introduction Project of Kunming University(Grant No.XJ20220003)Yunnan Provincial Natural Science Basic Research Fund General Program(Grant No.202501AT070058).
文摘Auxin is a phytohormone that is critical for plant growth and development.The molecular mechanisms underlying auxin biosynthesis,transport,and signaling are well understood.However,the complex mechanism by which auxin regulates plant volatile biosynthesis has seldom been studied.A growing array of unique auxin-related plant volatiles have recently been discovered.This study comprehensively reviews recent findings on auxin and auxin-related genes and their roles in the formation of plant volatiles.This study highlights the implications of exogenous auxin application,genes involved in auxin signaling transduction,and hormonal crosstalk during volatile compound biosynthesis in plants.Plant hormones facilitate the integration of multiple volatile signals to enable specific and appropriate responses to environmental changes.This will improve our overall understanding of the role of auxins in plant volatile compound metabolic pathways.Recent studies have delineated the considerable advancements in elucidating the intricate methods by which plants employ auxin regulatory pathways to modulate the release of volatile chemicals during development and growth,along with prospective research paths.
基金supported by the National Nature Science Foundation of China(Grant No.31660568)Guangxi Science and Technology major project(Grant No.GuikeAA22068088)+2 种基金start-up funding for introduced talents in Guangxi University,the Guangxi Colleges and Universities Young and Middle-aged Teachers'Basic Scientific Research Ability Improvement Project(Grant No.2024KY0010)Guangxi Graduate Education Innovation Program(Grant No.YCSW2024093)the Guangxi University Student Innovation and Entrepreneurship Training Program Funding Project(Grant Nos.202310593704,202310593714,202410953044S).
文摘N6-methyladenosine(m^(6)A)modification,the most abundant internal modification in messenger RNA(mRNA)and long non-coding RNA(lncRNA),has emerged as a critical epitranscriptomic regulatory mechanism in eukaryotes.While the importance of m^(6)A modification in various biological processes has been recognized,a comprehensive understanding of its diverse roles in plant biology and agricultural applications remains fragmented.This review analyzes recent advances inm^(6)A modification's biological functions in plants.m^(6)A modification plays crucial roles in multiple aspects of plant life,including seed germination,organ development,and reproductive structure formation.Furthermore,m^(6)A has been found to significantly influence plant responses to environmental stresses,including salt,drought,temperature,and heavy metal exposure.We also uncover m^(6)A involvement in important agricultural traits.This review provides insights into the mechanistic understanding of m^(6)A modification in plants and highlights its applications in agricultural improvement,offering a foundation for future research in crop enhancement and stress resistance.
文摘For decades,Xu has been committed to fulfilling the duty and mission of a scientist and educator—diving into the laws of nature,caring deeply for the nation,and earnestly cultivating younger generations.
基金supported by the National Key Research and Development Program of China(No.2024YFD1701101)the Fund for Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA28010503)+2 种基金the National Natural Science Foundation of China(No.31971515)the Fund for National Key Research and Development Plan of China(No.2019YFC1804100)the Fund for Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(No.CAAS-ZDRW202110).
文摘Atrazine,a persistent triazine herbicide,poses environmental and health risks.This study examines the synergis-tic remediation of atrazine-contaminated soil using green manure plant(GMP)hairy vetch(Vicia villosa Roth,VV)and the exogenous atrazine-degrading bacterium Arthrobacter sp.ATR1.Soil samples contaminated with atrazine at 5 and 20 mg/kg were treated with control(CK),ATR1(CKatr),hairy vetch(VV),and combined hairy vetch and ATR1 remediation(VVatr).The results indicated that the VVatr treatment exhibited the most effective atrazine removal,achieving enhancements of 56.12%at 5 mg/kg and 54.51%at 20 mg/kg compared to CK after 28 days.Soil enzyme activities,including urease,sucrase,and alkaline phosphatase,were significantly elevated in the VV and VVatr treatments,contributing to improved soil quality.Additionally,the CKatr,VV,and VVatr treat-ments enhanced bacterial diversity and richness while altering the microbial community structure.The VV and VVatr treatments notably enriched indigenous atrazine-degrading bacteria and nitrogen-fixing bacteria in the rhizosphere.This microbial enrichment upregulated the Atrazine degradation and Nitrogen metabolism pathways,facilitating both atrazine removal and nitrogen cycling in the soil.And VVatr treatment promoted the stability of the microbial network and enhanced the cooperative relationship between key indigenous atrazine-degrading and nitrogen-fixing bacteria.These findings explain the mechanism of plantmicrobe combined remediation of atrazine-contaminated soil from the perspective of rhizosphere microorganisms and offer a theoretical basis for the practical application of this method.
基金the Chinese Academy of Sciences Research Center for Ecology and Environment of Central Asia(RCEECA),the construction and joint research for the China-Tajikistan“Belt and Road”Joint Laboratory on Biodiversity Conservation and Sustainable Use(2024YFE0214200)the Shanghai Cooperation Organization Partnership and International Technology Cooperation Plan of Science and Technology Projects(2023E01018,2025E01056)the Chinese Academy of Sciences President’s International Fellowship Initiative(PIFI)(2024VBC0006).
文摘Tajikistan represents a core region of the biodiversity hotspot in Central Asian mountains and has exceptional vascular plant diversity.However,the species diversity of the country faces urgent conservation challenges.There has been a lack of a comprehensive and multidimensional assessment to inform strategic conservation planning.Therefore,this study integrated 4 key biodiversity indices including species richness(SR),phylogenetic diversity(PD),threatened species richness(TSR),and endemic species richness(ESR)to map species diversity distribution patterns,identify conservation gaps,and elucidate their effects of climatic factors.This study revealed that species diversity shows a clear trend of decreasing from the western region to the eastern region of Tajikistan.The central–western mountains(specifically the Gissar-Darvasian and Zeravshanian regions)emerge as irreplaceable biodiversity hotspots.However,we found a severe spatial mismatch between these priority areas and the existing protected areas(PAs).Protection coverage for all hotspots was alarmingly low,ranging from 31.00%to 38.00%.Consequently,a critical 64.80%of integrated priority areas fall outside of the current PAs,representing a major conservation gap.This study identified precipitation seasonality and isothermality as the principal drivers,collectively explaining over 50.00%of the diversity variation and suggesting high vulnerability to hydrological shifts.Furthermore,we detected significant geographic sampling bias in the public biodiversity databases,with the most critical hotspot being systematically under-sampled.This study provides a robust scientific basis for conservation action,highlighting the urgent need to strategically expand PAs in the under-protected southwestern region and to mitigate critical sampling gaps through targeted data digitization and field surveys.These measures are indispensable for securing Tajikistan’s unique biodiversity and achieving the Kunming-Montreal Global Biodiversity Framework Target 3(“30×30 Protection”).
基金supported by the National Key Research and Development Program of China(2025YFE0103800,2023YFE0102600,2024YFE0214200).
文摘Livestock farming is a critical pillar of Tajikistan’s national economy and livelihood security.However,significant economic challenges in the country have led to the degradation of grassland ecosystems.This degradation has not only reduced the productivity of grassland ecosystems but also severely impacted their ecological functions.A particularly concerning consequence is the threat to biodiversity,as the survival and persistence of endemic,rare,and endangered plant species are at serious risk,thereby diminishing the value of species’genetic resources.Based on the data from multiple sources such as literature reviews,field observations,and national statistics,this study employed a systematic literature review and meta-analysis to investigate the current status,causes of degradation,and restoration measures for grassland ecosystems in Tajikistan.The results revealed that Tajikistan’s grassland ecosystems support exceptionally high plant species diversity,comprising over 4500 vascular plant species,including nearly 1500 endemic and sub-endemic taxa that constitute a unique genetic reservoir.These ecosystems are experiencing severe degradation,characterized by significantly reduced vegetation cover and declining species richness.Palatable forage species are increasingly being displaced by unpalatable,thorny,and poisonous species.The primary drivers of degradation include excessive grazing pressure,which disrupts plant reproductive cycles and regeneration capacity,habitat fragmentation due to urbanization and infrastructure development,and uncontrolled exploitation of medicinal and edible plants.Climate change,particularly rising temperatures and altered precipitation patterns,further exacerbates these anthropogenic pressures.Ecological restoration experiments suggested that both ecosystem productivity and plant species diversity are significantly enhanced by systematic reseeding trials using altitude-adapted native species.These findings underscore the necessity of establishing scientifically grounded approaches for ecological restoration.
基金Supported by Science and Technology Project of China Tobacco Zhejiang Industrial Co.,Ltd.(2023330000340093).
文摘[Objectives]To investigate the effects of different planting densities and nitrogen application rates on the yield and quality of the tobacco cultivar Chuxue 80.[Methods]A field experiment was conducted in Hubei Province,evaluating various combinations of planting density and nitrogen rate for Chuxue 80.[Results]At the maturity stage,the TN1 treatment(5 kg N per 667 m^(2) with a density of 1900 plants per 667 m^(2))demonstrated the most favorable agronomic performance.The TN9 treatment(11 kg N per 667 m^(2) with a density of 1110 plants per 667 m^(2))achieved the highest wrapper tobacco yield and output value.Meanwhile,the TN5 treatment(8 kg N per 667 m^(2) with a density of 1515 plants per 667 m^(2))resulted in the best smoking quality.[Conclusions]The TN9 treatment,with a planting density of 1110 plants per 667 m^(2) and a nitrogen application rate of 11 kg per 667 m^(2),is recommended as the optimal cultivation practice for Chuxue 80 in Hubei Province.
基金supported by the Guizhou Provincial Science and Technology Project(Grant No.[2022]091).
文摘Toxic heavy metal and metalloid(THMM)contamination poses a major global challenge,threatening human health and sustainable agriculture.The crucial role of the Cytochrome P450(CYP)gene family in plant tolerance to THMMs has been recently highlighted,but there is still a lack of comprehensive understanding,especially in relation to metabolites.This study delved into the identification of CYP genes that are linked to the tolerance mechanisms of plants in response to heavy metal stress.The findings highlight the significant metabolic pathways that contribute to this resilience,using rice and Arabidopsis as exemplars.THMM exposure changed CYP gene expression in plants,and THMM antidotes mitigated its downregulation and that of flavonoid biosynthesis genes.CYP genes involved in THMM responses were predominantly enriched in the pathways associated with flavonoid synthesis,indicating functional adaptations to distinct stresses.Notably,anthocyanin(Ant)accumulation,a type of flavonoid,affected the uptake of various heavy metals in Brassica rapa,with flavonoid biosynthesis-associated genes correlating with Cd or As tolerance and Ant content.These findings highlight the critical importance of flavonoid metabolism and the intricate network of biosynthesis genes in bolstering plant resilience against heavy metal stress.This enhanced understanding paves the way for significant advancements in phytoremediation technologies,offering innovative solutions for soil and water decontamination.
基金funded from the Natural Science Foundation of Heilongjiang Province(LH2024C095,YQ2023B001)the China Postdoctoral Science Foundation(2024M751241)+3 种基金the National Key Laboratory of Green Pesticides(Central China Normal University)the earmarked fund for China Agricultural Research System(CARS170503)Heilongjiang Province Agriculture Research SystemEcological Agriculture 20231197Heilongjiang Province“Double First Class”Discipline Collaborative Innovation Achievement Project(LJGXCG2023-036).
文摘Reactive oxygen species(ROS)act as early messengers in plants exposed to drought,salinity,heat and other environmental challenges.Their timely removal is crucial.Unchecked ROS injure membranes,macromolecules and photosynthetic systems,ultimately curbing growth or causing cell death.While mitochondria possess inhouse antioxidant machinery,how non-mitochondrial systems contribute to mitochondrial redox homeostasis has remained unresolved.Laura F.DiGiovanni et al.demonstrate that peroxisomes directly protect mitochondria through contact-mediated ROS shuttling.This discovery extends the concept of organelle crosstalk beyond metabolic exchange to contact-mediated ROS flux,adding a system-level buffer against oxidative stress.Deep understanding and regulation of this pathway are highly significant for exploring how ROS coordinate plant stress responses,enhancing crop stress resistance and reducing extreme environment-induced oxidative damage.This may provide breeders and agronomists with a novel approach to develop stress-resistant traits.
基金supported by the Anhui Province Tongxin Science and Technology Innovation Project(202523b11020014)the Anhui Province Higher Education Quality Engineering Program(2024fwxx003).
文摘SalicS1 is a genetically encoded,ratiometric FRET biosensor that brings salicylic acid(SA)research to the same real-time imaging standard long available for ABA and GA.Built through a modular Golden Gate platform and informed by NPR-NIMIN structural biology,SalicS1 achieves SA specificity,tunable affinity,reversibility,and non-perturbing expression in Arabidopsis.Using this sensor,pathogen infection,non-adapted fungal challenge,and aphid feeding are shown to elicit spatially propagating SA surges rather than purely local accumulation,revealing a tissue-level organization of immune signaling that bulk assays could not resolve.SalicS1 therefore provides a broadly deployable tool for dissecting the geometry,timing,and genotype dependence of SA-mediated plant defense.
基金funded by the Department of Education of Liaoning Province and was supported by the Basic Scientific Research Project of the Department of Education of Liaoning Province(Grant No.LJ222411632051)and(Grant No.LJKQZ2021085)Natural Science Foundation Project of Liaoning Province(Grant No.2022-BS-222).
文摘Virtual power plant(VPP)integrates a variety of distributed renewable energy and energy storage to participate in electricity market transactions,promote the consumption of renewable energy,and improve economic efficiency.In this paper,aiming at the uncertainty of distributed wind power and photovoltaic output,considering the coupling relationship between power,carbon trading,and green cardmarket,the optimal operationmodel and bidding scheme of VPP in spot market,carbon trading market,and green card market are established.On this basis,through the Shapley value and independent risk contribution theory in cooperative game theory,the quantitative analysis of the total income and risk contribution of various distributed resources in the virtual power plant is realized.Moreover,the scheduling strategies of virtual power plants under different risk preferences are systematically compared,and the feasibility and accuracy of the combination of Shapley value and independent risk contribution theory in ensuring fair income distribution and reasonable risk assessment are emphasized.A comprehensive solution for virtual power plants in the multi-market environment is constructed,which integrates operation strategy,income distribution mechanism,and risk control system into a unified analysis framework.Through the simulation of multi-scenario examples,the CPLEXsolver inMATLAB software is used to optimize themodel.The proposed joint optimization scheme can increase the profit of VPP participating in carbon trading and green certificate market by 29%.The total revenue of distributed resources managed by VPP is 9%higher than that of individual participation.
文摘Arid and semi-arid ecosystems are prone to extensive fires due to specific climatic conditions,sparse vegetation cover,and high density of fine fuels.Understanding the flammability characteristics of land covers is essential for fire management and designing land restoration programs in arid and semi-arid ecosystems.This study provided a new approach to evaluate the flammability of shrublands and woodlands using flammability indices(FIs)including time to ignition(TI),duration of combustion(DC),and flame height(FH)of plant species and their relative frequencies in the Dalfard Basin of southeastern Iran.The results showed that there was a significant difference in FIs between land covers.Shrublands had higher flammability potential compared with woodlands.Plant moisture content had a negative relationship with TI(P<0.010)and no significant relationship with DC and FH(P>0.050).Artemisia spp.,Astragalus gossypinus Fischer,Amygdalus scoparia Spach,and Cymbopogon jwarancusa(Jones)Schult.had the highest FI.Tree species such as Rhazya stricta Decne.,and Pistacia atlantica Desf.showed greater resistance to fire.Using principal component analysis,the relationship between species and FIs was examined,and TI of wet fuel was the most important FI in relation to species.Structural equation model showed that life form(P<0.001)was the most important flammability driver.Precipitation(P<0.010)and legume species(P<0.010)were significantly related to the flammability in arid land.This study emphasizes the importance of managing high-risk species and using resistant species in vegetation restoration and shows that combining species FIs with their abundance is an effective tool for assessing fire risk and fuel management at the plant community scale.
基金supported by Science and Technology Project of SGCC(Research on Distributed Cooperative Control of Virtual Power Plants Based on Hybrid Game)(5700-202418337A-2-1-ZX).
文摘This paper suggests a way to improve teamwork and reduce uncertainties in operations by using a game theory approach involving multiple virtual power plants(VPP).A generalized credibility-based fuzzy chance constraint programming approach is adopted to address uncertainties stemming from renewable generation and load demand within individual VPPs,while robust optimization techniques manage electricity and thermal price volatilities.Building upon this foundation,a hierarchical Nash-Stackelberg game model is established across multiple VPPs.Within each VPP,a Stackelberg game resolves the strategic interaction between the operator and photovoltaic prosumers(PVP).Among VPPs,a cooperative Nash bargaining model coordinates alliance formation.The problem is decomposed into two subproblems:maximizing coalitional benefits,and allocating cooperative surpluses via payment bargaining,solved distributively using the alternating direction method of multipliers(ADMM).Case studies demonstrate that the proposed strategy significantly enhances the economic efficiency and uncertainty resilience of multi-VPP alliances.
基金financially supported by the National Key R&D Program of China(2021YFD1901203 and 2021YFD1901204)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA0440404)+2 种基金the National Natural Science Foundation of China(42377348)the Science Foundation for Distinguished Young Scholars of Hunan Province,China(2024JJ2052)the Natural Science Foundation of Guangxi,China(2025GXNSFAA069337)。
文摘Rapidly improving infertile croplands and enhancing their soil organic carbon(SOC)pool necessitate substantial organic materials incorporation.Converting loose crop straw into granulated form facilitates uniform incorporation within the plough soil layer.As an innovative soil amelioration approach,the efficiency and patterns of SOC accumulation remain unclear.Two field experiments were conducted in infertile subtropical upland and paddy soils with 0,30,60,and 90 Mg ha^(-1)granulated straw incorporation.After one year,SOC accumulation efficiency from straw input remained stable in upland(30.8–37.5%)with increasing amounts of straw incorporation,while declined from 60.0 to 38.3%in paddy.In both croplands,the contributions of lignin phenols to SOC increased with increasing straw incorporation,while the contributions from amino sugars remained constant at higher straw input levels.Subsequently,the ratios of lignin phenols to amino sugars increased with increasing straw incorporation,indicating faster plant residue accumulation compared to microbial necromass,as the granulation approach limited microbial involvement in straw transformation.Thus,single-time incorporation of substantial granulated straw presents an effective agricultural strategy for rapid amelioration of infertile croplands.
基金supported by the National Key Research and Development Program of China(2021YFD2100700).
文摘Triacylglycerol(TAG)components in human milk,infant formulas with different fat sources,and plant oils(palm oil,flaxseed oil,sunflower oil,corn oil,soybean oil,coconut oil,low erucic acid rapeseed oil,and high oleic acid rapeseed oil)were analyzed and compared using ultraperformance supercritical fluid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPSFC-Q-TOF-MS).Distinct TAG profiles were identified by lipidomics,principal component analysis(PCA),and heatmap visualization.Human milk was characterized by a high abundance of medium-and long-chain triacylglycerols(MLCTs),saturated-unsaturated-unsaturated(SUU)-type TAGs(sn-2 palmitic acid).In contrast,plant oils lacked MLCTs and SUU-type TAGs,containing mainly TAGs esterified long-chain polyunsaturated fatty acid.Breast milk contains about 27%MLCT,about 50%SUU-type triacylglycerols(which contain about 34%UPU-type triacylglycerols),which is significantly different from plant oils,structured fats and infant formulas.These findings provide a clear basis for optimizing the fat blend in infant formula to better mimic the unique TAG profile of human milk,thereby improving energy delivery and nutrient absorption for infants.
基金supported by the National Natural Science Foundation of China(Grant Nos.32072630,32372774,and U22A20499)the earmarked fund for CARS(Grant No.CARS-19-01A).
文摘Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement.WRKY transcription factors regulate various plant processes,including growth and development,stress responses,and metabolite biosynthesis.However,the molecular network through which WRKY coordinates these pathways in tea plants remains unclear.In this study,we revealed that CsWRKY57L,a cold-inducible WRKY IIc subfamily member,positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants.Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor(CBF)-cold-responsive(COR)gene pathway.In contrast,heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect.Additionally,overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis.Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a,CsSWEET15,and AtSWEET15,which encode sugar transporters essential for plant reproductive development,and inhibits their transcription.Overall,the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression.This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants,providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.
文摘The main purpose of using geothermal energy piles(GEPs)is to enable the exploitation of geothermal energy for meeting the heating/cooling demands of buildings efficiently.However,the installation process of conventional GEPs is inconvenient compared with that of traditional foundation piles.The pre-bored grouted planted geothermal energy pile(PGP GEP)is an innovative technology to simplify the installation process.Most investigations of in-situ experiments for conventional GEPs have focused on summer conditions.An in-situ test for a PGP GEP was conducted to analyze the temperature changes and thermo-mechanical characteristics under winter conditions.The results show that the average temperature of the pile decreased by 5.1℃,and the pile exhibited a general trend of high temperatures at both ends and low temperatures in the middle.In mechanics,strong pile end restraints resulted in smaller observed axial strain and higher axial thermal-induced force in the pile ends than at the middle of the pile.
