In this study, an （AG） n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO （ fast Isolation by AFLP sequences containing repeats） method. Among 356 positive colonies verifi...In this study, an （AG） n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO （ fast Isolation by AFLP sequences containing repeats） method. Among 356 positive colonies verified by colony PCR of the （AG）n microsatellite-enriched library, 233 colonies were selected for further sequencing. According to the results, 202 colonies containing microsatellites were obtained, accounting for 86.7%. Discarding sequences with miscella- neous peaks, overlapping peaks and weak signals, 39 sequences were screened finally for subsequent primer exploitation, including 22 perfect type SSR loci, 5 im- perfect type SSR loci and 12 compound type SSR loci. The FIASCO method is efficient and feasible for constructing microsatellite-enriched libraries of Rhododendron spp. , which laid a solid foundation for isolation of microsatellite loci and investigation of genetic diversity and genetic structure of Rhododendron spp.展开更多
Background:Actinidia chinensis Planch.roots(AcRoots)have been applied as an anti-inflammatory and antitumor drug in the treatment of gastric cancer(GC).However,their mechanisms against GC cells remain unclear.To inves...Background:Actinidia chinensis Planch.roots(AcRoots)have been applied as an anti-inflammatory and antitumor drug in the treatment of gastric cancer(GC).However,their mechanisms against GC cells remain unclear.To investigate the anticancer effect of AcRoots in GC and the possible underlying mechanism by using network pharmacology.Methods:Differentially expressed genes between gastric precancerous lesions and cancer were analyzed in Gene Expression Omnibus datasets,and these genes were overlapped with potential targets of AcRoots.Potential targets and pathways for AcRoots treatment of GC predicted by network pharmacology.Furthermore,we used the GC cell line HGC27 to explore the molecular mechanisms in the context of hub genes in apoptosis,invasion,metastasis,and epithelial to mesenchymal transition-promoting factors.Molecular docking between hub targets and active drug components was also performed.Results:Network pharmacological analysis suggested that the potential mechanism was related to the Wnt pathway and predicted nine hub genes.In in vitro studies,AcRoots significantly decreased HGC27 cell viability and promoted apoptosis by upregulating caspase3 and downregulating Bcl2.Moreover,it suppressed invasion and metastasis as well as the expression of epithelial to mesenchymal transition-related factors.In addition,AcRoots affected the phosphorylation level of GSK3β(Ser9)in the Wnt pathway to promote the degradation ofβ-catenin,resulting in the downregulation of the downstream target genes c-myc,cyclin D1 and snail.All the experimental results were consistent with the network pharmacology results.Conclusion:This study combined network pharmacology with in vitro experiments to provide valid evidence for the clinical promotion of AcRoots.展开更多
[Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid rat...[Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid ratio,ultrasonic temperature,ultrasonic time and ultrasonic power as influencing factors.[Results]The optimal extraction process was as follows:solid/liquid ratio 1∶15(g/mL),ultrasonic temperature 50℃,ultrasonic time 50 min and ultrasonic power 90 W.Under the optimal extraction conditions,the extraction rate of polysaccharides reached 2.05%.[Conclusions]The method is simple and feasible,and can be used to optimize the extraction process of polysaccharides in A.arguta,in order to provide a theoretical basis for the further development and utilization of related resources.展开更多
In order to offer scientific base for the cultivation of Parthenocissus quinquefolia planch in barren mountainous areas, the paper studied the light response characteristics of the photosynthetic rate （Pn）, transpir...In order to offer scientific base for the cultivation of Parthenocissus quinquefolia planch in barren mountainous areas, the paper studied the light response characteristics of the photosynthetic rate （Pn）, transpiration rate （Tt） and water use efficiency （WUE） that two-year-old P. quinquefolia planch leaf has under different soil moisture conditions with CIRAS-2 portable photosynthesis system made in PPS corporation in England. The results indicated that the Pn, Tr and WUE of P. quinquefolia planch leaf had notable threshold of responses to the variation of soil moisture and photosynthetic active radiation （PAR）. The Po of P. quinquefolia planch was at a relatively high level when the volumetric water content （VWC） was 12.6%-20.7% and relative water content （RWC） 45.2%-74.3%. When VWC was about 18.3%, the Pn and Tr reached the highest value while the WUE was relatively low. When the VWC was about 12.6% （RWC was about 45.2%）, the WUE reached the highest level. The fitting soil moisture range which could keep Pn and WUE at relatively high level was 12.6%-18.3% for VWC and 45.2%-65.7% for RWC. Out of this range, Pn and WUE would descend obviously. In the fitting range of VWC, the Pn and WUE maintained a high level when the PAR range was 400-1000 umol·m^-2·s^-1, otherwise, the WUE would fall, and the most suitable PAR was 800 umol·m^-2·s^-1. In view of the above results, we conclude that the P. quinquefolia planch can adapt to a wide range of illumination intensity. It has the property of strong drought-resistance but can not stand the high soil moisture environment. Therefore, it is a suitable vine for the afforestation in barren mountainous areas.展开更多
目的 探究我国主产区猕猴桃与新西兰猕猴桃的品质差异,并对其进行综合性评价。方法 本研究以我国主产区和新西兰猕猴桃果实为实验材料,分析猕猴桃果实的表型性状、营养成分和感官评定指标,采用系统性描述、熵权-逼近理想解排序(techniqu...目的 探究我国主产区猕猴桃与新西兰猕猴桃的品质差异,并对其进行综合性评价。方法 本研究以我国主产区和新西兰猕猴桃果实为实验材料,分析猕猴桃果实的表型性状、营养成分和感官评定指标,采用系统性描述、熵权-逼近理想解排序(technique for order preference by similarity to ideal solution, TOPSIS)法,对猕猴桃进行分析和综合评价。结果 不同产区猕猴桃果实的品质指标存在显著差异,红心猕猴桃为云南和陕西的可溶性固形物含量最高,分别为15.9%和15.5%,四川的总酸最低为0.86%,云南和四川的可溶性糖含量均值最高分别为62.2%和59.5%,云南的果糖和葡萄糖含量均值最高,分别为15.1%和17.7%。绿心猕猴桃为中国产区维生素C含量的显著高于新西兰;湖南的可溶性固形物含量最高(18.0%),固酸比均值最高的是湖南(14.5)和重庆(14.4),可溶性糖含量均值最高的是重庆(58.2%),但是新西兰的果糖和葡萄糖含量均值最高,分别为14.2%和16.4%。黄心猕猴桃为新西兰的维生素C、可溶性固形物、固酸比、可溶性糖、果糖、葡萄糖含量均值都是最高(分别为0.89 mg/g、16.7%、14.3、63.6%、15.6%、17.7%),但是四川(3.73%)与河南(3.61%)的蔗糖含量比新西兰高。结论 研究表明我国主产区猕猴桃与新西兰猕猴桃存在品质差异。通过熵权-TOPSIS模型评价结果显示,红心猕猴桃得分前2名为云南和四川的红阳猕猴桃,绿心猕猴桃得分最高为陕西的翠香猕猴桃,新西兰的海沃德猕猴桃排名第4,黄心猕猴桃得分最高为新西兰的阳光金果猕猴桃,其次是四川和云南的G3猕猴桃。本研究通过“感官-成分-表型”三维关联模型,验证了多指标综合评价的有效性,为构建猕猴桃品质评价体系提供了方法论支撑。展开更多
基金Supported by Doctoral Starting up Foundation of Huanggang Normal University(2013030903)Opening Fund of Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resource Comprehensive Utilization(2013000403)
文摘In this study, an （AG） n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO （ fast Isolation by AFLP sequences containing repeats） method. Among 356 positive colonies verified by colony PCR of the （AG）n microsatellite-enriched library, 233 colonies were selected for further sequencing. According to the results, 202 colonies containing microsatellites were obtained, accounting for 86.7%. Discarding sequences with miscella- neous peaks, overlapping peaks and weak signals, 39 sequences were screened finally for subsequent primer exploitation, including 22 perfect type SSR loci, 5 im- perfect type SSR loci and 12 compound type SSR loci. The FIASCO method is efficient and feasible for constructing microsatellite-enriched libraries of Rhododendron spp. , which laid a solid foundation for isolation of microsatellite loci and investigation of genetic diversity and genetic structure of Rhododendron spp.
基金funded by the National Natural Science Foundation of China(Project No.81973615,No.81803910).
文摘Background:Actinidia chinensis Planch.roots(AcRoots)have been applied as an anti-inflammatory and antitumor drug in the treatment of gastric cancer(GC).However,their mechanisms against GC cells remain unclear.To investigate the anticancer effect of AcRoots in GC and the possible underlying mechanism by using network pharmacology.Methods:Differentially expressed genes between gastric precancerous lesions and cancer were analyzed in Gene Expression Omnibus datasets,and these genes were overlapped with potential targets of AcRoots.Potential targets and pathways for AcRoots treatment of GC predicted by network pharmacology.Furthermore,we used the GC cell line HGC27 to explore the molecular mechanisms in the context of hub genes in apoptosis,invasion,metastasis,and epithelial to mesenchymal transition-promoting factors.Molecular docking between hub targets and active drug components was also performed.Results:Network pharmacological analysis suggested that the potential mechanism was related to the Wnt pathway and predicted nine hub genes.In in vitro studies,AcRoots significantly decreased HGC27 cell viability and promoted apoptosis by upregulating caspase3 and downregulating Bcl2.Moreover,it suppressed invasion and metastasis as well as the expression of epithelial to mesenchymal transition-related factors.In addition,AcRoots affected the phosphorylation level of GSK3β(Ser9)in the Wnt pathway to promote the degradation ofβ-catenin,resulting in the downregulation of the downstream target genes c-myc,cyclin D1 and snail.All the experimental results were consistent with the network pharmacology results.Conclusion:This study combined network pharmacology with in vitro experiments to provide valid evidence for the clinical promotion of AcRoots.
基金National Undergraduate Innovation and Entrepreneurship Training Program of China(202111430049).
文摘[Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid ratio,ultrasonic temperature,ultrasonic time and ultrasonic power as influencing factors.[Results]The optimal extraction process was as follows:solid/liquid ratio 1∶15(g/mL),ultrasonic temperature 50℃,ultrasonic time 50 min and ultrasonic power 90 W.Under the optimal extraction conditions,the extraction rate of polysaccharides reached 2.05%.[Conclusions]The method is simple and feasible,and can be used to optimize the extraction process of polysaccharides in A.arguta,in order to provide a theoretical basis for the further development and utilization of related resources.
基金The project was supported by national key science and technology item in "10th five-year" period (2001BA510B0404), and Shandong superior industrial itemin "breeding and industrial exploitation of superior liana, adapting to afforesting barren mountain".
文摘In order to offer scientific base for the cultivation of Parthenocissus quinquefolia planch in barren mountainous areas, the paper studied the light response characteristics of the photosynthetic rate （Pn）, transpiration rate （Tt） and water use efficiency （WUE） that two-year-old P. quinquefolia planch leaf has under different soil moisture conditions with CIRAS-2 portable photosynthesis system made in PPS corporation in England. The results indicated that the Pn, Tr and WUE of P. quinquefolia planch leaf had notable threshold of responses to the variation of soil moisture and photosynthetic active radiation （PAR）. The Po of P. quinquefolia planch was at a relatively high level when the volumetric water content （VWC） was 12.6%-20.7% and relative water content （RWC） 45.2%-74.3%. When VWC was about 18.3%, the Pn and Tr reached the highest value while the WUE was relatively low. When the VWC was about 12.6% （RWC was about 45.2%）, the WUE reached the highest level. The fitting soil moisture range which could keep Pn and WUE at relatively high level was 12.6%-18.3% for VWC and 45.2%-65.7% for RWC. Out of this range, Pn and WUE would descend obviously. In the fitting range of VWC, the Pn and WUE maintained a high level when the PAR range was 400-1000 umol·m^-2·s^-1, otherwise, the WUE would fall, and the most suitable PAR was 800 umol·m^-2·s^-1. In view of the above results, we conclude that the P. quinquefolia planch can adapt to a wide range of illumination intensity. It has the property of strong drought-resistance but can not stand the high soil moisture environment. Therefore, it is a suitable vine for the afforestation in barren mountainous areas.
文摘目的 探究我国主产区猕猴桃与新西兰猕猴桃的品质差异,并对其进行综合性评价。方法 本研究以我国主产区和新西兰猕猴桃果实为实验材料,分析猕猴桃果实的表型性状、营养成分和感官评定指标,采用系统性描述、熵权-逼近理想解排序(technique for order preference by similarity to ideal solution, TOPSIS)法,对猕猴桃进行分析和综合评价。结果 不同产区猕猴桃果实的品质指标存在显著差异,红心猕猴桃为云南和陕西的可溶性固形物含量最高,分别为15.9%和15.5%,四川的总酸最低为0.86%,云南和四川的可溶性糖含量均值最高分别为62.2%和59.5%,云南的果糖和葡萄糖含量均值最高,分别为15.1%和17.7%。绿心猕猴桃为中国产区维生素C含量的显著高于新西兰;湖南的可溶性固形物含量最高(18.0%),固酸比均值最高的是湖南(14.5)和重庆(14.4),可溶性糖含量均值最高的是重庆(58.2%),但是新西兰的果糖和葡萄糖含量均值最高,分别为14.2%和16.4%。黄心猕猴桃为新西兰的维生素C、可溶性固形物、固酸比、可溶性糖、果糖、葡萄糖含量均值都是最高(分别为0.89 mg/g、16.7%、14.3、63.6%、15.6%、17.7%),但是四川(3.73%)与河南(3.61%)的蔗糖含量比新西兰高。结论 研究表明我国主产区猕猴桃与新西兰猕猴桃存在品质差异。通过熵权-TOPSIS模型评价结果显示,红心猕猴桃得分前2名为云南和四川的红阳猕猴桃,绿心猕猴桃得分最高为陕西的翠香猕猴桃,新西兰的海沃德猕猴桃排名第4,黄心猕猴桃得分最高为新西兰的阳光金果猕猴桃,其次是四川和云南的G3猕猴桃。本研究通过“感官-成分-表型”三维关联模型,验证了多指标综合评价的有效性,为构建猕猴桃品质评价体系提供了方法论支撑。
