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RNAi介导的pirin低表达降低了K562细胞的红系分化能力
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作者 毛群铨 王婕 +4 位作者 曹玲玲 薛建有 戚武林 朱晓芳 张世馥 《基础医学与临床》 CSCD 北大核心 2013年第7期888-891,共4页
目的研究pirin低表达对人红白血病细胞系K562红系分化和细胞活力的影响。方法通过短发卡RNA(shRNA)干扰技术,构建pirin低表达细胞稳定株,用Western blot方法对干扰效果进行检测,并用联苯胺染色和MTT法分别检测干扰pirin后对K562细胞的... 目的研究pirin低表达对人红白血病细胞系K562红系分化和细胞活力的影响。方法通过短发卡RNA(shRNA)干扰技术,构建pirin低表达细胞稳定株,用Western blot方法对干扰效果进行检测,并用联苯胺染色和MTT法分别检测干扰pirin后对K562细胞的红系分化和增殖的影响。结果转染了pirin-shRNA的细胞其pirin的表达量明显低于对照组(随机干扰组),pirin低表达与对照组相比对细胞增殖无影响,但在诱导剂诱导分化过程中,血红蛋白的合成出现了显著性的降低(P<0.05)。结论 Pirin低表达不影响K562细胞的增殖能力,但能降低其红系分化能力。 展开更多
关键词 K562细胞 慢病毒 pirin 红系分化 白血病
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Pirin对脑胶质瘤干细胞增殖和自我更新的作用
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作者 蒋海璐 刘峰 +3 位作者 董文慧 吴瑾 满江红 段小涛 《国际药学研究杂志》 CSCD 北大核心 2017年第10期953-957,969,共6页
目的研究铁结合核蛋白(Pirin)对脑胶质瘤干细胞(GSC)增殖和自我更新的作用,为恶性胶质瘤的治疗提供新思路。方法构建PLKO.1-sh Pirin慢病毒质粒,利用慢病毒感染细胞的体系,在GSC中对Pirin蛋白的表达进行稳定干涉,采用Western印迹法检测P... 目的研究铁结合核蛋白(Pirin)对脑胶质瘤干细胞(GSC)增殖和自我更新的作用,为恶性胶质瘤的治疗提供新思路。方法构建PLKO.1-sh Pirin慢病毒质粒,利用慢病毒感染细胞的体系,在GSC中对Pirin蛋白的表达进行稳定干涉,采用Western印迹法检测Pirin蛋白的干涉效果,然后通过细胞活力变化反映对GSC增殖能力的影响,运用肿瘤细胞球形成实验检测Pirin对GSC自我更新能力的影响。结果 Pirin蛋白在GSC细胞中高表达,且GSC比非干性肿瘤细胞(NSTC)培养上清中含有更多Pirin蛋白。PLKO.1-sh Pirin慢病毒质粒构建成功,2条干涉序列都能明显敲低GSC中Pirin蛋白的表达。在GSC中稳定敲低Pirin表达,GSC的细胞活力和肿瘤细胞球形成能力受到明显抑制。结论敲低Pirin蛋白能明显抑制GSC的增殖和自我更新能力。 展开更多
关键词 铁结合核蛋白(pirin) 脑胶质瘤干细胞 细胞增殖 肿瘤细胞球
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Recent behavior and possible future evolution of the glacieret in the cirque Golemiya Kazan in the Pirin Mountains under conditions of climate warming 被引量:1
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作者 Peter NOJAROV Emil GACHEV Karsten GRUNEWALD 《Journal of Mountain Science》 SCIE CSCD 2019年第1期16-29,共14页
This research reveals relationships between climate variables and inter-annual dynamics in the area of the glacieret located in the cirque Golemiya Kazan in the Pirin Mountains. The study period is 1993–2017. The cor... This research reveals relationships between climate variables and inter-annual dynamics in the area of the glacieret located in the cirque Golemiya Kazan in the Pirin Mountains. The study period is 1993–2017. The correlations are identified using statistical methods. Also, a statistical model is constructed, including some climate variables as predictors. Despite the evident decrease of the glacieret's size in the period from the 1950 s onwards, the long-term trends for the last decades have been insignificant. The main climatic factors influencing the inter-annual dynamics in the area of the glacieret are air temperature, precipitation, zonal and meridional winds and relative humidity. With respect to the dynamics in the area of the glacieret, the important trends in the different climate variables are those of the warm period air temperatures and zonal(u) wind. They also determine to a great extent its future development by acting in two opposite directions – rising temperatures in the warm period will lead to a rapid decrease of its area by the end of the melting season, while the change of wind directionfrom west to east in the warm period will increase its area. The influence of the zonal wind in the warm period is explained mainly by the location of the glacieret in the cirque. Generally, the glacieret is tilted downwards from west to east. Thus, westerly winds facilitate blowing away the snow from the surface of the glacieret, assisting its melting in the warm period. Easterly winds do not have such an effect. The combination of the opposite effects of these two most important climate variables leads to the most likely scenario for the future development of the glacieret, according to which by the middle of this century it is expected to turn into a semi-permanent snow patch, which disappears after some summers, and by the end of the century to completely melt every year before the end of the melting season. 展开更多
关键词 CLIMATE WARMING Glacieret pirin MOUNTAINS Statistical modeling Glacieret area PROJECTION
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Sildenafil plus Low Dose Aspirin for Prevention of Preeclampsia: A Randomized Controlled Trial
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作者 Mahmoud Mohamed Ghaleb Youssef Sobhy Labib Karim Ahmed Wahba 《Open Journal of Obstetrics and Gynecology》 2021年第2期189-209,共21页
<strong>Objective</strong><strong>:</strong><span style="font-family:""><span style="font-family:Verdana;"> To compare between the efficacy of the use of or... <strong>Objective</strong><strong>:</strong><span style="font-family:""><span style="font-family:Verdana;"> To compare between the efficacy of the use of oral sildenafil plus low dose aspirin versus the use of oral low dose aspirin alone in pregnancy as preventive measure in women at risk for preeclampsia (PE). </span><b><span style="font-family:Verdana;">Design: </span></b><span style="font-family:Verdana;">A randomized clinical trial. </span><b><span style="font-family:Verdana;">Setting: </span></b><span style="font-family:Verdana;">Outpatient Obstetric clinic of Ain Shams University Maternity Hospital. </span><b><span style="font-family:Verdana;">Population or sample: </span></b><span style="font-family:Verdana;">Women at gestational age of </span></span><span style="font-family:""><span style="font-family:Verdana;">≤</span><span><span style="font-family:Verdana;">16 weeks who at risk for PE between June 2018 and June 2019. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> Participants were randomly allocated into two groups: Group I Included 200 women who received a 25 mg tablet of oral sildenafil citrate tid until delivery plus 100 mg tablet of aspirin orally once daily until gestational age of 36 weeks, Group II Included 200 women who received a 100 mg tablet of aspirin orally once daily until gestational age of 36 weeks. </span><b><span style="font-family:Verdana;">Main Outcome Measures: </span></b><span style="font-family:Verdana;">Incidence of preeclampsia diagnosed per ACOG criteria. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> The incidence of PE in both groups showed no statistically significant difference. The incidence of PE in the first group is 11.0%, and it is 12.0% in the second group (p-value 0.754). </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> The addition of sildenafil citrate to low dose aspirin had no impact on the prevention of preeclampsia for women at risk of PE, in addition, sildenafil did not improve maternal and fetal outcomes.</span></span></span> 展开更多
关键词 PREECLAMPSIA Hypertension Placental Disease Pregnancy Complications As-pirin Sildenafil Citrate
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阿司匹林对灯盏细辛注射液中咖啡酸的药代动力学影响 被引量:5
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作者 戴国梁 刘史佳 +4 位作者 李长印 吴磊 马世堂 居文政 谈恒山 《中国药理学通报》 CAS CSCD 北大核心 2014年第4期570-574,共5页
目的研究阿司匹林对灯盏细辛注射液(DI)中咖啡酸的药代动力学的影响。方法 LC-MS/MS测定大鼠血浆中咖啡酸浓度,实验动物分为对照组和实验组,对照组单独尾静脉给予DI,实验组尾静脉给予DI及灌胃给予阿司匹林。DAS 1.0药动学软件计算药动... 目的研究阿司匹林对灯盏细辛注射液(DI)中咖啡酸的药代动力学的影响。方法 LC-MS/MS测定大鼠血浆中咖啡酸浓度,实验动物分为对照组和实验组,对照组单独尾静脉给予DI,实验组尾静脉给予DI及灌胃给予阿司匹林。DAS 1.0药动学软件计算药动学参数。结果 DI单独给药、DI-阿司匹林联合给药,咖啡酸在体内代谢动力学模型均为二室开放模型,联合给药后,咖啡酸的T12β明显增大,CL有所减小。结论阿司匹林可减慢咖啡酸在体内的代谢过程。 展开更多
关键词 灯盏细辛注射液 咖啡酸 阿司匹林 大鼠 血浆 代动力学 LC—MS MS
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Mountain Water Tower and Ecological Risk Estimation of the Mesta-Nestos Transboundary River Basin (Bulgaria-Greece) 被引量:2
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作者 K. Grunewald J. Scheithauer +1 位作者 J.-M. Monget N. Nikolova 《Journal of Mountain Science》 SCIE CSCD 2007年第3期209-220,共12页
The Mesta-Nestos river basin in Bulgaria and Greece is a case study for transboundary decision-making support in south-eastern Europe and a show-case for the development of methodologies and information-gathering for ... The Mesta-Nestos river basin in Bulgaria and Greece is a case study for transboundary decision-making support in south-eastern Europe and a show-case for the development of methodologies and information-gathering for the integrated regional planning of water resources. Land-use conflicts in this water-scarce region cover a wide spectrum of activities like agricultural irrigation, drinking water production, diversions for industrial water, and risk of pollution from mining, to name a few examples. Measurements of the water quality were carried out in the upper basin. Results will be illustrated by the example of the environmental situation in the alpine region of the Pirin National Park as well as in the Razlog Basin with a stronger anthropogenic impact and pollution around a former uranium mine near the village of Elesnica. The social and economic development of this transboundary region is a recently established priority for mean an increase in water usage the water resources if regional the future. It will and more stress for impacts of global climate change are verified. Problem-focused management of the catchment area as a whole on the basis of proved geo-data sets is needed for the future. 展开更多
关键词 Environment interactions NUTRIENTS POLLUTANTS resource management Southeast Europe uranium water quality pirin high Mountains
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阿司匹林诱导宫颈癌Hela细胞凋亡及抑制增殖的机制研究
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作者 刘健 崔艳艳 +4 位作者 李胜泽 刘红丽 李玉芝 郭苏阳 刘静 《中国临床药理学与治疗学》 CAS CSCD 2016年第11期1263-1266,共4页
目的:探讨阿司匹林对于宫颈癌Hela细胞的凋亡诱导作用以及增殖抑制的相关机制。方法:通过离体培养宫颈癌Hela细胞,以不同剂量的阿司匹林对其增殖过程进行干预,利用Annexin V-FITC/PI凋亡试剂盒检测Hela细胞的凋亡情况。同时,采用Western... 目的:探讨阿司匹林对于宫颈癌Hela细胞的凋亡诱导作用以及增殖抑制的相关机制。方法:通过离体培养宫颈癌Hela细胞,以不同剂量的阿司匹林对其增殖过程进行干预,利用Annexin V-FITC/PI凋亡试剂盒检测Hela细胞的凋亡情况。同时,采用Western-blot法检测NF-κB p65、Bcl-2以及Bax蛋白的表达情况。最后,通过碘化丙啶(PI)染色测定宫颈癌Hela细胞周期分布情况。结果:阿司匹林1.0、5.0、10.0 mmol/L剂量组能够显著增加宫颈癌Hela细胞的凋亡率,其S期、G_2/M期细胞所占比例明显减少,同时G_0、G_1期细胞分布明显提高。NF-κB p65蛋白、Bcl-2蛋白表达水平显著降低,其下降程度与所接受阿司匹林剂量有明显相关性,同时Bax蛋白表达水平提高。结论:阿司匹林能够通过抑制NF-κB p65、Bcl-2蛋白表达水平并提高Bax蛋白表达水平,干预相关细胞周期分布最终诱导宫颈癌Hela细胞的凋亡,其诱导机制的强弱程度呈现明显的剂量相关性。 展开更多
关键词 宫颈癌 HELA细胞 阿司匹林 细胞凋亡
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阿司匹林术前服用对冠脉搭桥手术凝血的影响 被引量:1
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作者 邵月娟 《天津医科大学学报》 2004年第4期560-562,共3页
目的 :利用Sonoclot分析仪评价抗血小板药物阿司匹林对体外循环冠脉搭桥手术凝血功能的影响。方法 :选择持续服用阿司匹林至术前3天冠脉搭桥手术患者21例(阿司匹林组) ,与同期术前两周内未服用阿司匹林患者19例(对照组) ,分别于体外循... 目的 :利用Sonoclot分析仪评价抗血小板药物阿司匹林对体外循环冠脉搭桥手术凝血功能的影响。方法 :选择持续服用阿司匹林至术前3天冠脉搭桥手术患者21例(阿司匹林组) ,与同期术前两周内未服用阿司匹林患者19例(对照组) ,分别于体外循环前、后采取中心静脉血测定Sonoclot指标ACT、CR、PF及常规凝血指标PLT、Fib、APTT、PT ,同时记录术后24h输血和胸腔引流量。结果 :体外循环 (CPB)前两组Sonoclot和常规凝血指标均在正常范围内 ,CPB后Sonoclot显示ACT延长 ,CR下降 ,PF降低(P<0.01) ;常规凝血显示PT、APTT延长 ,Fib水平降低 ,PLT数量减少(P<0.01) ,组间比较无显著性差异(P>0.05)。阿司匹林组术后24h胸引量显著高于对照组(P<0.01) ,输血量组间无显著差异。结论 :术前服用阿司匹林会导致体外循环冠脉搭桥患者术后出血增多 ,Sonoclot分析仪不能用于此类患者凝血异常的评估。 展开更多
关键词 体外循环 冠脉搭桥手术 凝血 阿司匹林
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铝镁匹林+氢氯吡格雷用药方案在急性缺血性脑卒中治疗中的临床价值
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作者 曾泓滔 《系统医学》 2024年第22期101-104,共4页
目的探讨对急性缺血性脑卒中患者应用铝镁匹林+氢氯吡格雷用药方案展开治疗的临床价值。方法非随机选取2021年5月—2024年5月高州市人民医院收治的60例急性缺血性脑卒中患者作为研究对象;以不同治疗方法分为两组,各30例。参照组使用氢... 目的探讨对急性缺血性脑卒中患者应用铝镁匹林+氢氯吡格雷用药方案展开治疗的临床价值。方法非随机选取2021年5月—2024年5月高州市人民医院收治的60例急性缺血性脑卒中患者作为研究对象;以不同治疗方法分为两组,各30例。参照组使用氢氯吡格雷治疗,研究组在参照组基础上使用铝镁匹林治疗。对比两组患者治疗总有效率、美国国立卫生研究院卒中量表评分、丙二醛、超氧化物歧化酶、异构前列腺素水平。结果研究组治疗总有效率为96.67%(29/30),高于参照组的73.33%(22/30),差异有统计学意义(χ^(2)=6.405,P<0.05)。治疗后,研究组美国国立卫生研究院卒中量表评分低于参照组,差异有统计学意义(P<0.05)。治疗后,研究组的丙二醛、超氧化物歧化酶、异构前列腺素水平均优于参照组,差异有统计学意义(P均<0.05)。结论急性缺血性脑卒中患者应用铝镁匹林+氢氯吡格雷用药方案治疗可明显提升患者的治疗效果,改善患者的神经功能、脂质过氧化水平以及氧化应激反应水平。 展开更多
关键词 氢氯吡格雷 铝镁匹林 急性缺血性脑卒中 应用效果
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