Objective:By using the method of network pharmacology to explore the mechanism of action of Caulis Sinomenii-Caulis Piperis Kadsurae herb pair in the treatment of rheumatoid arthritis.Methods:Search and screen the act...Objective:By using the method of network pharmacology to explore the mechanism of action of Caulis Sinomenii-Caulis Piperis Kadsurae herb pair in the treatment of rheumatoid arthritis.Methods:Search and screen the active ingredients and targets of Qingfengteng(Caulis Sinomenii)and Haifengteng(Caulis Piperis Kadsurae)through the TCMSP database,and predict and supplement the target points of the active ingredients through the Swiss Target Prediction platform;collect rheumatoid arthritis disease targets through GeneCards,OMIM database,and DRUGBANK database,construct a Venn diagram,and obtain the key target of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs in the treatment of rheumatoid arthritis;use Cytoscape 3.7.1 to establish the effect of Caysaceae sylvestris and Rhizoma sibirica in the treatment of rheumatoid arthritis"Drug-active ingredient-target"network diagram,submit key targets to STRING11.0 database to construct a protein interaction network model to obtain core targets;perform the GO and KEGG enrichment analysis of key targets on the Metascape platform,and draw the network diagram of"active pharmaceutical ingredients-targets-pathways".Results:After screening,discover a total of 27 active ingredients of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs,which can act on 229 targets,1269 related targets for rheumatoid arthritis diseases.And obtain a total of 95 common targets for drug active ingredients and diseases,including PTGS2,EGFR,MMP9,CASP3,JUN,etc.The common targets are mainly enriched in signaling pathways such as Pathways in cancer,Th17 cell differentiation and Endocrine resistance etc.Conclusion:This study preliminarily explored the potential mechanism of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs in the treatment of rheumatoid arthritis.The Caulis Sinomenii-Caulis Piperis Kadsurae herb pair may regulate cancer-related pathways,Th17 cell differentiation,endocrine resistance and other pathways to treat rheumatoid arthritis by regulating targets such as PTGS2,EGFR,and MMP9,providing clues and ideas for follow-up experimental research.展开更多
文摘Objective:By using the method of network pharmacology to explore the mechanism of action of Caulis Sinomenii-Caulis Piperis Kadsurae herb pair in the treatment of rheumatoid arthritis.Methods:Search and screen the active ingredients and targets of Qingfengteng(Caulis Sinomenii)and Haifengteng(Caulis Piperis Kadsurae)through the TCMSP database,and predict and supplement the target points of the active ingredients through the Swiss Target Prediction platform;collect rheumatoid arthritis disease targets through GeneCards,OMIM database,and DRUGBANK database,construct a Venn diagram,and obtain the key target of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs in the treatment of rheumatoid arthritis;use Cytoscape 3.7.1 to establish the effect of Caysaceae sylvestris and Rhizoma sibirica in the treatment of rheumatoid arthritis"Drug-active ingredient-target"network diagram,submit key targets to STRING11.0 database to construct a protein interaction network model to obtain core targets;perform the GO and KEGG enrichment analysis of key targets on the Metascape platform,and draw the network diagram of"active pharmaceutical ingredients-targets-pathways".Results:After screening,discover a total of 27 active ingredients of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs,which can act on 229 targets,1269 related targets for rheumatoid arthritis diseases.And obtain a total of 95 common targets for drug active ingredients and diseases,including PTGS2,EGFR,MMP9,CASP3,JUN,etc.The common targets are mainly enriched in signaling pathways such as Pathways in cancer,Th17 cell differentiation and Endocrine resistance etc.Conclusion:This study preliminarily explored the potential mechanism of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs in the treatment of rheumatoid arthritis.The Caulis Sinomenii-Caulis Piperis Kadsurae herb pair may regulate cancer-related pathways,Th17 cell differentiation,endocrine resistance and other pathways to treat rheumatoid arthritis by regulating targets such as PTGS2,EGFR,and MMP9,providing clues and ideas for follow-up experimental research.
