Climate change has caused fluctuations in the frequency and severity of droughts,favoring extended periods of drought associated with anthropic actions and triggering other stressful abiotic effects that threaten terr...Climate change has caused fluctuations in the frequency and severity of droughts,favoring extended periods of drought associated with anthropic actions and triggering other stressful abiotic effects that threaten terrestrial ecosystems.As climate warming intensifies,drought is a major challenge for forest growth.Pine(Pinus Linn.)is an important genus of forest in the Northern Hemisphere and has a certain tolerance to drought.This article analyzes and reviews the advances in research about drought stress of major Pinus spp.plants in recent years and discusses understanding and future core problems.To adapt to water-deficient environments,pine plants adapt to drought by changing growth traits,closing some stomata on leaves,changing the growth and structure of roots,and adjusting their physiological activities.Moreover,the expression of specific genes is altered,causing changes in the expression of several signaling molecules and metabolites to counteract drought stress.展开更多
Pinus koraiensis is the dominant and constructive species of the zonal vegetation in Northeast China,known as the mixed broadleaf-Korean pine forest.Although carbohydrate metabolism pathways in the seed embryo are kno...Pinus koraiensis is the dominant and constructive species of the zonal vegetation in Northeast China,known as the mixed broadleaf-Korean pine forest.Although carbohydrate metabolism pathways in the seed embryo are known to play crucial roles during seed dormancy and germination in P.koraiensis,it remains unclear whether these metabolic pathways function differentially across tissues.P.koraiensis seeds that had undergone different durations of moist chilling in their natural environment,yielding seeds with relatively deeper primary physiological dormancy(DDS)and seeds with released primary physiological dormancy(RDS).A non-targeted metabolomic analysis was conducted on the radicle and hypocotyl-cotyledon portions of both DDS and RDS,before and after a two-week incubation under favorable conditions.Under germination conditions,RDS and DDS showed divergent metabolic profiles,especially regarding carbohydrate metabolism.Specifically,RDS seeds showed significantly reduced substrates of respiratory metabolic pathways in both radicles and hypocotyl-cotyledons.Conversely,the intermediates of the carbohydrate metabolism pathway(particularly the tricarboxylic acid cycle)accumulating in radicles of DDS seeds under germination conditions.Moreover,in RDS,the carbohydrate metabolic pathways were more prevalent in the hypocotyl-cotyledon,while lysine degradation and ascorbate and aldarate metabolism were the dominant metabolic pathways in radicles.In contrast,the tricarboxylic acid cycle showed higher activity in DDS radicles compared to hypocotyl-cotyledons.We further demonstrated that carbohydratemetabolic pathways continue to play a dominant role in both dormancy maintenance and germination processes of P.koraiensis seeds.Notably,the carbohydratemetabolism in radicles likely exerts more critical regulatory functions in these two physiological processes compared to that in cotyledon and hypocotyl tissues.展开更多
The defense mechanisms induced in wild Chinese pine(Pinus tabuliformis)in response to herbivores are not well characterized,especially in the field.To address this knowledge gap,we established a biological model syste...The defense mechanisms induced in wild Chinese pine(Pinus tabuliformis)in response to herbivores are not well characterized,especially in the field.To address this knowledge gap,we established a biological model system to evaluate proteome variations in pine needles after feeding by the Chinese pine caterpillar(Dendrolimus tabulaeformis),a major natural enemy and dominant herbivore.Quantitative tandem mass tag(TMT)proteomics and bioinformatics were utilized to systematically identify differentially abundant proteins implicated in the induced defense response of Chinese pine.We validated key protein changes using parallel reaction monitoring(PRM)technology.Pathway analysis revealed that the induced defenses involved phenylpropanoid,coumarin,and flavonoid biosynthesis,among other processes.To elucidate the regulatory patterns underlying pine resistance,we determined the activities of defense enzymes and levels of physiological and biochemical compounds.In addition,the expression of upstream genes for key proteins was validated by qRT-PCR.Our results provide new molecular insights into the induced defense mechanisms in Chinese pine against this caterpillar in the field.A better understanding of these defense strategies will inform efforts to breed more-resistant pine varieties.展开更多
Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short...Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.展开更多
Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis ind...Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis induction,and immune-modulatory activities of extracts from three herbal medicines with historical use in traditional medicine—Acanthopanax sessiliflorus,Phragmites communis,and Pinus densiflora,as well as their combined extract(GMAS 01/COM),on human lung cancer cells(A549)and normal cell lines,including murine macrophages(RAW 264.7)and human keratinocytes(HaCaT).Methods:Plant extracts were prepared using aqueous extraction,sonication,and rotary evaporation.The total phenolic and flavonoid contents were quantified using the Folin-Ciocalteu and AlCl3 colorimetric methods,respectively.Antioxidant potential was evaluated via 2,2-Diphenyl-1-picrylhydrazyl(DPPH)scavenging and reducing power assays.Cytotoxicity was assessed using an MTT assay,while reactive oxygen species(ROS)generation was quantified using a 2′,7′-Dichlorodihydrofluorescein diacetate(DCFH-DA)assay.Anticancer properties,including colony formation inhibition and migration suppression,were examined using colony formation and wound healing assays.The expression of apoptotic and inflammatory mediators was analysed through qPCR.Results:GMAS 01 selectively induced apoptosis in A549 cells without cytotoxic effects on RAW264.7 and HaCaT cells.Mechanistically,it elevated intracellular ROS and activated the intrinsic mitochondrial apoptotic pathway,evidenced by p53 upregulation,increased Bax,and decreased Bcl-2 expression.GMAS 01 also significantly inhibited colony formation and migration in A549 cells.In RAW264.7 cells,it reduced nitric oxide(NO)production and downregulated iNOS,COX-2,IL-6,and IL-8,indicating strong immunomodulatory activity.Conclusion:GMAS 01 exhibits potent antioxidant,anti-inflammatory,and anticancer effects,likely mediated through ROS-induced mitochondrial apoptosis.However,mechanistic interpretations are limited by the absence of protein-level validation and pathway inhibition studies.Upcoming studies should aim to verify the underlying mechanisms and evaluate their potential for real-world application.展开更多
Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter...Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.展开更多
Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization...Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization treatment.Method:Bioinformatics methods,including gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,were used to identify genes related to survival prognosis in hepatocellular carcinoma(HCC)patients.A retrospective analysis of 115 advanced liver cancer patients treated between January 2016 and October 2017 was conducted.Patients were categorized into SPP1 high-expression(n=89)and low-expression groups(n=26).Additionally,115 healthy individuals served as the control group.The relationship between SPP1 expression and clinical pathological features was analyzed.A 60-month follow-up and logistic regression analysis identified risk factors affecting survival.Results:SPP1 mRNA expression was significantly higher in liver cancer patients compared to healthy controls(P<0.05).SPP1 expression levels were significantly associated with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging(P<0.05).High SPP1 expression,along with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,were independent risk factors for survival(P<0.05).The 60-month survival rate was 17.39%,with a median survival of 40 months in the low-expression group versus 18 months in the high-expression group(P<0.05).Conclusion:SPP1 expression is significantly upregulated in advanced liver cancer patients and has predictive value for postoperative survival following hepatic artery chemoembolization treatment.SPP1,combined with clinical indicators such as tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,may serve as a prognostic biomarker for interventional treatment outcomes.展开更多
The level of genetic variation within a breeding population affects the effectiveness of selection strategies for genetic improvement.The relationship between genetic variation level within Pinus tabuliformis breeding...The level of genetic variation within a breeding population affects the effectiveness of selection strategies for genetic improvement.The relationship between genetic variation level within Pinus tabuliformis breeding populations and selection strategies or selection effectiveness is not fully investigated.Here,we compared the selection effectiveness of combined and individual direct selection strategies using half-and full-sib families produced from advanced-generation P.tabuliformis seed orchard as our test populations.Our results revealed that,within half-sib families,average diameter at breast height(DBH),tree height,and volume growth of superior individuals selected by the direct selection strategy were higher by 7.72%,18.56%,and 31.01%,respectively,than those selected by the combined selection strategy.Furthermore,significant differences(P<0.01)were observed between the two strategies in terms of the expected genetic gains for average tree height and volume.In contrast,within full-sib families,the differences in tree average DBH,height,and volume between the two selection strategies were relatively minor with increase of 0.17%,2.73%,and 2.21%,respectively,and no significant differences were found in the average expected genetic gains for the studied traits.Half-sib families exhibited greater phenotypic and genetic variation,resulting in improved selection efficiency with the direct selection strategy but also introduced a level of inbreeding risk.Based on genetic distance estimates using molecular markers,our comparative seed orchard design analysis showed that the Improved Adaptive Genetic Programming Algorithm(IAPGA)reduced the average inbreeding coefficient by 14.36% and 14.73% compared to sequential and random designs,respectively.In conclusion,the combination of the direct selection strategy with IAPGA seed orchard design aimed at minimizing inbreeding offered an efficient approach for establishing advanced-generation P.tabuliformis seed orchards.展开更多
Pine nuts are becoming a popular snack because of their interesting taste and positive nutritional profile. Their fatty acid profile has been reported but there is some confusion identifying named cultivars. This stud...Pine nuts are becoming a popular snack because of their interesting taste and positive nutritional profile. Their fatty acid profile has been reported but there is some confusion identifying named cultivars. This study presents the fatty acid profile of five different cultivars of pine nuts currently growing in the South Island of New Zealand. The data are compared to three different samples of pine nuts currently imported into NZ. Identification of the twelve different fatty acids extracted from these samples was identified by retention time using GC-FID and GC-MS methods. The peaks were further identified by comparison of the retention times with a MS Library match and their corresponding LRI value. All but two of the extracted fatty acids were identified by comparisons with a known pure fatty acid standard sample for each fatty acid. Botanical identification of the five locally grown pine nuts was confirmed by calculating the Diagnostic Index of each cultivar from its fatty acid composition.展开更多
基金the National Natural Science Foundation of China(31960301)the Guizhou Provincial Characteristic Key Laboratory(QJHKY[2021]002).
文摘Climate change has caused fluctuations in the frequency and severity of droughts,favoring extended periods of drought associated with anthropic actions and triggering other stressful abiotic effects that threaten terrestrial ecosystems.As climate warming intensifies,drought is a major challenge for forest growth.Pine(Pinus Linn.)is an important genus of forest in the Northern Hemisphere and has a certain tolerance to drought.This article analyzes and reviews the advances in research about drought stress of major Pinus spp.plants in recent years and discusses understanding and future core problems.To adapt to water-deficient environments,pine plants adapt to drought by changing growth traits,closing some stomata on leaves,changing the growth and structure of roots,and adjusting their physiological activities.Moreover,the expression of specific genes is altered,causing changes in the expression of several signaling molecules and metabolites to counteract drought stress.
基金supported by the National Natural Science Foundation of China(31901300).
文摘Pinus koraiensis is the dominant and constructive species of the zonal vegetation in Northeast China,known as the mixed broadleaf-Korean pine forest.Although carbohydrate metabolism pathways in the seed embryo are known to play crucial roles during seed dormancy and germination in P.koraiensis,it remains unclear whether these metabolic pathways function differentially across tissues.P.koraiensis seeds that had undergone different durations of moist chilling in their natural environment,yielding seeds with relatively deeper primary physiological dormancy(DDS)and seeds with released primary physiological dormancy(RDS).A non-targeted metabolomic analysis was conducted on the radicle and hypocotyl-cotyledon portions of both DDS and RDS,before and after a two-week incubation under favorable conditions.Under germination conditions,RDS and DDS showed divergent metabolic profiles,especially regarding carbohydrate metabolism.Specifically,RDS seeds showed significantly reduced substrates of respiratory metabolic pathways in both radicles and hypocotyl-cotyledons.Conversely,the intermediates of the carbohydrate metabolism pathway(particularly the tricarboxylic acid cycle)accumulating in radicles of DDS seeds under germination conditions.Moreover,in RDS,the carbohydrate metabolic pathways were more prevalent in the hypocotyl-cotyledon,while lysine degradation and ascorbate and aldarate metabolism were the dominant metabolic pathways in radicles.In contrast,the tricarboxylic acid cycle showed higher activity in DDS radicles compared to hypocotyl-cotyledons.We further demonstrated that carbohydratemetabolic pathways continue to play a dominant role in both dormancy maintenance and germination processes of P.koraiensis seeds.Notably,the carbohydratemetabolism in radicles likely exerts more critical regulatory functions in these two physiological processes compared to that in cotyledon and hypocotyl tissues.
基金supported by the Science and Technology Development Program of Hebei Agricultural University,the Research on Molecular Mechanisms of Population Differentiation and Adaptation of Forest Pests and Insects under Environmental Stress(grant No.:30771739)Forest Pests and Diseases(grant No.:1528003)the National Natural Science Foundation of China for the study of community regulatory mechanisms of insect pest pandemics in larch plantation forests(Grant No.:32371882).
文摘The defense mechanisms induced in wild Chinese pine(Pinus tabuliformis)in response to herbivores are not well characterized,especially in the field.To address this knowledge gap,we established a biological model system to evaluate proteome variations in pine needles after feeding by the Chinese pine caterpillar(Dendrolimus tabulaeformis),a major natural enemy and dominant herbivore.Quantitative tandem mass tag(TMT)proteomics and bioinformatics were utilized to systematically identify differentially abundant proteins implicated in the induced defense response of Chinese pine.We validated key protein changes using parallel reaction monitoring(PRM)technology.Pathway analysis revealed that the induced defenses involved phenylpropanoid,coumarin,and flavonoid biosynthesis,among other processes.To elucidate the regulatory patterns underlying pine resistance,we determined the activities of defense enzymes and levels of physiological and biochemical compounds.In addition,the expression of upstream genes for key proteins was validated by qRT-PCR.Our results provide new molecular insights into the induced defense mechanisms in Chinese pine against this caterpillar in the field.A better understanding of these defense strategies will inform efforts to breed more-resistant pine varieties.
基金supported by the National Research Foundation of Korea(2020R1F1A1074155).
文摘Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.
文摘Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis induction,and immune-modulatory activities of extracts from three herbal medicines with historical use in traditional medicine—Acanthopanax sessiliflorus,Phragmites communis,and Pinus densiflora,as well as their combined extract(GMAS 01/COM),on human lung cancer cells(A549)and normal cell lines,including murine macrophages(RAW 264.7)and human keratinocytes(HaCaT).Methods:Plant extracts were prepared using aqueous extraction,sonication,and rotary evaporation.The total phenolic and flavonoid contents were quantified using the Folin-Ciocalteu and AlCl3 colorimetric methods,respectively.Antioxidant potential was evaluated via 2,2-Diphenyl-1-picrylhydrazyl(DPPH)scavenging and reducing power assays.Cytotoxicity was assessed using an MTT assay,while reactive oxygen species(ROS)generation was quantified using a 2′,7′-Dichlorodihydrofluorescein diacetate(DCFH-DA)assay.Anticancer properties,including colony formation inhibition and migration suppression,were examined using colony formation and wound healing assays.The expression of apoptotic and inflammatory mediators was analysed through qPCR.Results:GMAS 01 selectively induced apoptosis in A549 cells without cytotoxic effects on RAW264.7 and HaCaT cells.Mechanistically,it elevated intracellular ROS and activated the intrinsic mitochondrial apoptotic pathway,evidenced by p53 upregulation,increased Bax,and decreased Bcl-2 expression.GMAS 01 also significantly inhibited colony formation and migration in A549 cells.In RAW264.7 cells,it reduced nitric oxide(NO)production and downregulated iNOS,COX-2,IL-6,and IL-8,indicating strong immunomodulatory activity.Conclusion:GMAS 01 exhibits potent antioxidant,anti-inflammatory,and anticancer effects,likely mediated through ROS-induced mitochondrial apoptosis.However,mechanistic interpretations are limited by the absence of protein-level validation and pathway inhibition studies.Upcoming studies should aim to verify the underlying mechanisms and evaluate their potential for real-world application.
基金supported by the Joint Funds of the National Natural Science Foundation of China(U21A20272)。
文摘Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.
基金Medical Research Project of Xi’an Science and Technology Bureau“Molecular Mechanism of miR-1305 Competitive Endogenous circRNA in the Development of Liver Cancer”(Project No.22YXYJ0134)General Project of Key Research and Development Program of Shaanxi Provincial Department of Science and Technology“Mechanism Study on the Inhibition of Liver Cancer Invasion and Metastasis by Downregulating METTL3 and Reducing the m6A Modification Level of MMP3 with Honokiol”(Project No.2023-YBSF-631)。
文摘Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization treatment.Method:Bioinformatics methods,including gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,were used to identify genes related to survival prognosis in hepatocellular carcinoma(HCC)patients.A retrospective analysis of 115 advanced liver cancer patients treated between January 2016 and October 2017 was conducted.Patients were categorized into SPP1 high-expression(n=89)and low-expression groups(n=26).Additionally,115 healthy individuals served as the control group.The relationship between SPP1 expression and clinical pathological features was analyzed.A 60-month follow-up and logistic regression analysis identified risk factors affecting survival.Results:SPP1 mRNA expression was significantly higher in liver cancer patients compared to healthy controls(P<0.05).SPP1 expression levels were significantly associated with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging(P<0.05).High SPP1 expression,along with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,were independent risk factors for survival(P<0.05).The 60-month survival rate was 17.39%,with a median survival of 40 months in the low-expression group versus 18 months in the high-expression group(P<0.05).Conclusion:SPP1 expression is significantly upregulated in advanced liver cancer patients and has predictive value for postoperative survival following hepatic artery chemoembolization treatment.SPP1,combined with clinical indicators such as tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,may serve as a prognostic biomarker for interventional treatment outcomes.
基金financially supported by the Biological BreedingNational Science and Technology Major Project(2023ZD0405806)the National Key R&D Program for the 14th Five-Year Plan in China(2022YFD2200304).
文摘The level of genetic variation within a breeding population affects the effectiveness of selection strategies for genetic improvement.The relationship between genetic variation level within Pinus tabuliformis breeding populations and selection strategies or selection effectiveness is not fully investigated.Here,we compared the selection effectiveness of combined and individual direct selection strategies using half-and full-sib families produced from advanced-generation P.tabuliformis seed orchard as our test populations.Our results revealed that,within half-sib families,average diameter at breast height(DBH),tree height,and volume growth of superior individuals selected by the direct selection strategy were higher by 7.72%,18.56%,and 31.01%,respectively,than those selected by the combined selection strategy.Furthermore,significant differences(P<0.01)were observed between the two strategies in terms of the expected genetic gains for average tree height and volume.In contrast,within full-sib families,the differences in tree average DBH,height,and volume between the two selection strategies were relatively minor with increase of 0.17%,2.73%,and 2.21%,respectively,and no significant differences were found in the average expected genetic gains for the studied traits.Half-sib families exhibited greater phenotypic and genetic variation,resulting in improved selection efficiency with the direct selection strategy but also introduced a level of inbreeding risk.Based on genetic distance estimates using molecular markers,our comparative seed orchard design analysis showed that the Improved Adaptive Genetic Programming Algorithm(IAPGA)reduced the average inbreeding coefficient by 14.36% and 14.73% compared to sequential and random designs,respectively.In conclusion,the combination of the direct selection strategy with IAPGA seed orchard design aimed at minimizing inbreeding offered an efficient approach for establishing advanced-generation P.tabuliformis seed orchards.
文摘Pine nuts are becoming a popular snack because of their interesting taste and positive nutritional profile. Their fatty acid profile has been reported but there is some confusion identifying named cultivars. This study presents the fatty acid profile of five different cultivars of pine nuts currently growing in the South Island of New Zealand. The data are compared to three different samples of pine nuts currently imported into NZ. Identification of the twelve different fatty acids extracted from these samples was identified by retention time using GC-FID and GC-MS methods. The peaks were further identified by comparison of the retention times with a MS Library match and their corresponding LRI value. All but two of the extracted fatty acids were identified by comparisons with a known pure fatty acid standard sample for each fatty acid. Botanical identification of the five locally grown pine nuts was confirmed by calculating the Diagnostic Index of each cultivar from its fatty acid composition.
