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轨道参数对钢轨Pinned-Pinned振动的影响 被引量:4
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作者 张攀 王安斌 +2 位作者 王志强 徐宁 张喆玉 《城市轨道交通研究》 北大核心 2016年第12期72-76,82,共6页
有研究认为钢轨不连续支撑造成的轮轨柔度差变是钢轨波浪磨耗产生和发展的主要原因之一。在此基础上对轨道参数对钢轨Pinned-Pinned振动的影响进行进一步分析。通过利用ANSYS软件建立轨道结构的有限元分析模型,计算分析了不连续支撑条... 有研究认为钢轨不连续支撑造成的轮轨柔度差变是钢轨波浪磨耗产生和发展的主要原因之一。在此基础上对轨道参数对钢轨Pinned-Pinned振动的影响进行进一步分析。通过利用ANSYS软件建立轨道结构的有限元分析模型,计算分析了不连续支撑条件下轨道的支撑刚度、支撑间距和结构阻尼对钢轨柔度特性的影响,并提出了控制钢轨波磨形成与发展的建议。 展开更多
关键词 轨道 pinnedpinned振动 支撑间距 支撑刚度 阻尼
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Shaking table test and numerical analysis of a 1:12 scale model of a special concentrically braced steel frame with pinned connections 被引量:7
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作者 Yu Haifeng Zhang Wenyuan +1 位作者 Zhang Yaochun Sun Yusong 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2010年第1期51-63,共13页
This paper describes shaking table tests of a 1:12 scale model of a special concentrically braced steel frame with pinned connections, which was fabricated according to a one-bay braced frame selected from a typical ... This paper describes shaking table tests of a 1:12 scale model of a special concentrically braced steel frame with pinned connections, which was fabricated according to a one-bay braced frame selected from a typical main factory building of a large thermal power plant. In order to investigate the seismic performance of this type of structure, several ground motion accelerations with different levels for seismic intensity Ⅷ, based on the Chinese Code for Seismic Design of Buildings, were selected to excite the model. The results show that the design methods of the members and the connections are adequate and that the structural system will perform well in regions of high seismicity. In addition to the tests, numerical simulations were also conducted and the results showed good agreement with the test results. Thus, the numerical model is shown to be accurate and the beam element can be used to model this structural system. 展开更多
关键词 concentrically braced steel frame pinned connections shaking table test numerical analysis seismic performance
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A dynamic photoresponse model for a pinned photodiode in CMOS image sensors 被引量:1
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作者 AO Jinghua GAO Zhiyuan +1 位作者 GAO Jing XU Jiangtao 《Optoelectronics Letters》 EI 2022年第7期419-424,共6页
A novel dynamic photoresponse model for complementary metal-oxide-semiconductor(CMOS)image sensors with pinned photodiode(PPD)structures is proposed.The PPD is regarded as the bonding structure of the two p-n junction... A novel dynamic photoresponse model for complementary metal-oxide-semiconductor(CMOS)image sensors with pinned photodiode(PPD)structures is proposed.The PPD is regarded as the bonding structure of the two p-n junctions.The transient current equation of the two junctions is calculated by the current-voltage formula of the p-n junction,and the photoresponse curve of the PPD is calculated and drawn by the numerical solution.Simulation results show that the dynamic model successfully restores the entire process of the electron accumulation in the PPD.The difference between the full well capacity(FWC)values which were calculated by the proposed model and the simulation results is less than 5%,which is much smaller than the error of 40%for the traditional model. 展开更多
关键词 PHOTORESPONSE pinned DYNAMIC
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A quantum efficiency analytical model for complementary metal–oxide–semiconductor image pixels with a pinned photodiode structure
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作者 曹琛 张冰 +2 位作者 吴龙胜 李娜 王俊峰 《Chinese Physics B》 SCIE EI CAS CSCD 2014年第12期254-262,共9页
A quantum efficiency analytical model for complementary metal–oxide–semiconductor(CMOS) image pixels with a pinned photodiode structure is developed. The proposed model takes account of the non-uniform doping dist... A quantum efficiency analytical model for complementary metal–oxide–semiconductor(CMOS) image pixels with a pinned photodiode structure is developed. The proposed model takes account of the non-uniform doping distribution in the N-type region due to the impurity compensation formed by the actual fabricating process. The characteristics of two boundary PN junctions located in the N-type region for the particular spectral response of a pinned photodiode, are quantitatively analyzed. By solving the minority carrier steady-state diffusion equations and the barrier region photocurrent density equations successively, the analytical relationship between the quantum efficiency and the corresponding parameters such as incident wavelength, N-type width, peak doping concentration, and impurity density gradient of the N-type region is established. The validity of the model is verified by the measurement results with a test chip of 160×160 pixels array,which provides the accurate process with a theoretical guidance for quantum efficiency design in pinned photodiode pixels. 展开更多
关键词 CMOS image sensor quantum efficiency pinned photodiode analytical model
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A low-power high-quality CMOS image sensor using 1.5 V 4T pinned photodiode and dual-CDS column-parallel single-slope ADC
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作者 Wenjing Xu Jie Chen +3 位作者 Zhangqu Kuang Li Zhou Ming Chen Chengbin Zhang 《Journal of Semiconductors》 EI CAS CSCD 2022年第8期53-59,共7页
This paper presents a low-power high-quality CMOS image sensor(CIS)using 1.5 V 4T pinned photodiode(4T-PPD)and dual correlated double sampling(dual-CDS)column-parallel single-slope ADC.A five-finger shaped pixel layer... This paper presents a low-power high-quality CMOS image sensor(CIS)using 1.5 V 4T pinned photodiode(4T-PPD)and dual correlated double sampling(dual-CDS)column-parallel single-slope ADC.A five-finger shaped pixel layer is proposed to solve image lag caused by low-voltage 4T-PPD.Dual-CDS is used to reduce random noise and the nonuniformity between columns.Dual-mode counting method is proposed to improve circuit robustness.A prototype sensor was fabricated using a 0.11μm CMOS process.Measurement results show that the lag of the five-finger shaped pixel is reduced by 80%compared with the conventional rectangular pixel,the chip power consumption is only 36 mW,the dynamic range is 67.3 dB,the random noise is only 1.55 e^(-)_(rms),and the figure-of-merit is only 1.98 e^(-)·nJ,thus realizing low-power and high-quality imaging. 展开更多
关键词 CMOS image sensor 4T pinned photodiode single-slope ADC correlated double sample counting method
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A review of recent theoretical and computational studies on pinned surface nanobubbles 被引量:1
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作者 刘亚伟 张现仁 《Chinese Physics B》 SCIE EI CAS CSCD 2018年第1期136-144,共9页
The observations of long-lived surface nanobubbles in various experiments have presented a theoretical challenge, as they were supposed to be dissolved in microseconds owing to the high Laplace pressure. However, an i... The observations of long-lived surface nanobubbles in various experiments have presented a theoretical challenge, as they were supposed to be dissolved in microseconds owing to the high Laplace pressure. However, an increasing number of studies suggest that contact line pinning, together with certain levels of oversaturation, is responsible for the anomalous stability of surface nanobubbles. This mechanism can interpret most characteristics of surface nanobubbles. Here, we summarize recent theoretical and computational work to explain how the surface nanobubbles become stable with contact line pinning. Other related work devoted to understanding the unusual behaviors of pinned surface nanobubbles is also reviewed here. 展开更多
关键词 surface nanobubble contact line pinning oversaturation lattice density functional theory
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Investigation of magnetization reversal process in pinned CoFeB thin film by in-situ Lorentz TEM 被引量:1
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作者 Ke Pei Wei-Xing Xia +5 位作者 Bao-Min Wang Xing-Cheng Wen Ping Sheng Jia-Ping Liu Xin-Cai Liu Run-Wei Li 《Chinese Physics B》 SCIE EI CAS CSCD 2018年第4期465-471,共7页
Exchange bias effect has been widely employed for various magnetic devices.The experimentally reported magnitude of exchange bias field is often smaller than that predicted theoretically,which is considered to be due ... Exchange bias effect has been widely employed for various magnetic devices.The experimentally reported magnitude of exchange bias field is often smaller than that predicted theoretically,which is considered to be due to the partly pinned spins of ferromagnetic layer by antiferromagnetic layer.However,mapping the distribution of pinned spins is challenging.In this work,we directly image the reverse domain nucleation and domain wall movement process in the exchange biased Co Fe B/Ir Mn bilayers by Lorentz transmission electron microscopy.From the in-situ experiments,we obtain the distribution mapping of the pinning strength,showing that only 1/6 of the ferromagnetic layer at the interface is strongly pinned by the antiferromagnetic layer.Our results prove the existence of an inhomogeneous pinning effect in exchange bias systems. 展开更多
关键词 exchange bias magnetization reversal process Lorentz transmission electron microscopy pinning effect distribution
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P型掺杂区工艺对Si基Pinned型光电二极管量子效率的影响 被引量:1
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作者 曹琛 张冰 +1 位作者 王俊峰 吴龙胜 《光电子.激光》 EI CAS CSCD 北大核心 2015年第1期54-62,共9页
为了更全面、系统地分析Si基Pinned型光电二极管(PPD,pinned photodiode)量子效率的工艺敏感特性,基于考虑表面(SRH,shockley-read-hall)复合率模型的时域有限差分数值模拟方法,对不同P+型表面层和P型外延(EPI,epitaxial)层工艺... 为了更全面、系统地分析Si基Pinned型光电二极管(PPD,pinned photodiode)量子效率的工艺敏感特性,基于考虑表面(SRH,shockley-read-hall)复合率模型的时域有限差分数值模拟方法,对不同P+型表面层和P型外延(EPI,epitaxial)层工艺条件下PPD可见光谱量子效率的变化特征及物理机制进行了研究。结果表明,P+型表面层离子束注入剂量和注入能量的增加分别引起非平衡载流子SRH复合率升高和PPD势垒区顶部下移,均可导致低于500nm波段量子效率的衰减,而后者进一步引起的势垒区纵向宽度缩减使该影响可持续至650nm波段;P型EPI掺杂浓度增加引起PPD势垒区底部上移,导致500~750nm波段量子效率的衰减;P型EPI厚度增加引起衬底强SRH复合区光电荷比重降低,导致高于700nm波段量子效率得到提升并趋向饱和。通过分析发现,Si基材料中光子吸收深度对波长的强依赖关系是导致两种P型掺杂区工艺条件对量子效率存在波段差异性影响的根本原因。 展开更多
关键词 pinned型光电二极管(PPD) 量子效率 工艺条件 数值模拟
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Pinch-off voltage modeling for CMOS image pixels with a pinned photodiode structure 被引量:1
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作者 曹琛 张冰 +2 位作者 吴龙胜 李炘 王俊峰 《Journal of Semiconductors》 EI CAS CSCD 2014年第7期90-96,共7页
A novel analytical model of pinch-off voltage for CMOS image pixels with a pinned photodiode structure is proposed. The derived model takes account of the gradient doping distributions in the N buried layer due to the... A novel analytical model of pinch-off voltage for CMOS image pixels with a pinned photodiode structure is proposed. The derived model takes account of the gradient doping distributions in the N buried layer due to the impurity compensation formed by manufacturing processes; the impurity distribution characteristics of two boundary PN junctions located in the region for particular spectrum response of a pinned photodiode are quantitative analyzed. By solving Poisson's equation in vertical barrier regions, the relationships between the pinch-off voltage and the corresponding process parameters such as peak doping concentration, N type width and doping concentration gradient of the N buried layer are established. Test results have shown that the derived model features the variations of the pinch-off voltage versus the process implant conditions more accurately than the traditional model. The research conclusions in this paper provide theoretical evidence for evaluating the pinch-off voltage design. 展开更多
关键词 pinned photodiode pixel design pinch-off voltage analytical model
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Measurement of charge transfer potential barrier in pinned photodiode CMOS image sensors 被引量:1
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作者 曹琛 张冰 +1 位作者 王俊峰 吴龙胜 《Journal of Semiconductors》 EI CAS CSCD 2016年第5期56-60,共5页
The charge transfer potential barrier (CTPB) formed beneath the transfer gate causes a noticeable image lag issue in pinned photodiode (PPD) CMOS image sensors (CIS), and is difficult to measure straightforwardl... The charge transfer potential barrier (CTPB) formed beneath the transfer gate causes a noticeable image lag issue in pinned photodiode (PPD) CMOS image sensors (CIS), and is difficult to measure straightforwardly since it is embedded inside the device. From an understanding of the CTPB formation mechanism, we report on an alternative method to feasibly measure the CTPB height by performing a linear extrapolation coupled with a horizontal left-shift on the sensor photoresponse curve under the steady-state illumination. The theoretical study was pertbrmed in detail on the principle of the proposed method. Application of the measurements oil a prototype PPD-CIS chip with an array of 160 ×160 pixels is demonstrated. Such a method intends to shine new light oil the guidance for the lag-free and high-speed sensors optimization based on PPD devices. 展开更多
关键词 CMOS image sensors (C1S) pinned photodiode (PPD) charge transfer potential barrier (CTPB) photoresponse curve
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A PINNED NETWORK OF EULER-BERNOULLI BEAMS UNDER FEEDBACK CONTROLS
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作者 ZHANG Kuiting XU Genqi 《Journal of Systems Science & Complexity》 SCIE EI CSCD 2013年第3期313-334,共22页
In this paper, the authors design boundary feedback controllers at the interior node to stabilize a star-shaped network of Euler-Bernoulli beams. The beams are pinned each other, that is, the displacements of the stru... In this paper, the authors design boundary feedback controllers at the interior node to stabilize a star-shaped network of Euler-Bernoulli beams. The beams are pinned each other, that is, the displacements of the structure are continuous but the rotations of the beams are not continuous. The weil-posed-ness of the closed loop system is proved by the semigroup theory. The authors show that the system is asymptotically stable if the authors impose a bending moment control on each edge. Finally, the authors derive the exponential stability of the system. 展开更多
关键词 Euler-Bernoulli beams pinned network semigroup theory stability.
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Photoelectric characteristics of an inverse U-shape buried doping design for crosstalk suppression in pinned photodiodes
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作者 曹琛 张冰 +2 位作者 李炘 吴龙胜 王俊峰 《Journal of Semiconductors》 EI CAS CSCD 2014年第11期81-89,共9页
A design of an inverse U-shape buried doping in a pinned photodiode (PPD) of CMOS image sensors is proposed for electrical crosstalk suppression between adjacent pixels. The architecture achieves no extra fill facto... A design of an inverse U-shape buried doping in a pinned photodiode (PPD) of CMOS image sensors is proposed for electrical crosstalk suppression between adjacent pixels. The architecture achieves no extra fill factor consumption, and proper built-in electric fields can be established according to the doping gradient created by the injections of the extremely low P-type doping buried regions in the epitaxial layer, causing the excess electrons to easily drift back to the photosensitive area rarely with a diffusion probability; the overall junction capacitance and photosensitive area extensions for a full well capacity (FWC) and internal quantum efficiency (IQE) improving are achieved by the injection of a buried N-type doping. By considering the image lag issue, the process parameters of all the injections have been precisely optimized. Optical simulation results based on the finite difference time domain method show that compared to the conventional PPD, the electrical crosstalk rate of the proposed architecture can be decreased by 60%-80% at an incident wavelength beyond 450 nm, IQE can be clearly improved at an incident wavelength between 400 and 600 nm, and the FWC can be enhanced by 107.5%. Furthermore, the image lag performance is sustained to a perfect low level. The present study provides important guidance on the design of ultra high resolution image sensors. 展开更多
关键词 CMOS image sensor electrical crosstalk photoelectric performance design pinned photodiode
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Z-pinned复合材料层板Ⅰ型裂纹的疲劳扩展
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作者 张阿盈 《结构强度研究》 2006年第3期30-37,共8页
用数值模拟方法研究z-pin增强复合材料DCB层压板Ⅰ型裂纹的疲劳扩展问题。在理论模型中,将z-pin简化为一系列施加在DCB层板上的外力,这些力在裂纹扩展中对裂纹提供桥接力。基于试验观察,桥接力随疲劳载荷的衰减规律可假设为一个分一... 用数值模拟方法研究z-pin增强复合材料DCB层压板Ⅰ型裂纹的疲劳扩展问题。在理论模型中,将z-pin简化为一系列施加在DCB层板上的外力,这些力在裂纹扩展中对裂纹提供桥接力。基于试验观察,桥接力随疲劳载荷的衰减规律可假设为一个分一。段的幂函数。用一个指数函数表示复合材料DCB层板疲劳断裂韧性与疲劳循环次数的关系,并将此作为裂纹扩展的判据。分析结果表明,无论是位移控制模式还是载荷控制模式,z—pin可以有效地降低分层扩展速率。还就z—pin密度,z-pin大小等参数进行了分析研究。 展开更多
关键词 z-pin增强DCB:z—pin桥接力 Ⅰ型裂纹 疲劳 裂纹扩展速率
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Electron Movements Pinned down to the Split Second
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作者 Mark Peplow 全勇 《当代外语研究》 2004年第11期24-22,共2页
1999年的诺贝尔化学奖颁发给了科学家艾哈迈德·译韦尔,以表彰他应用飞秒激光闪光成相技术观测到分子中的原子在化学反应中如何运动,从而为化学及相关科学带来了一场革命。随着飞秒化学领域研究的不断深入,它必将会进一步为科学发... 1999年的诺贝尔化学奖颁发给了科学家艾哈迈德·译韦尔,以表彰他应用飞秒激光闪光成相技术观测到分子中的原子在化学反应中如何运动,从而为化学及相关科学带来了一场革命。随着飞秒化学领域研究的不断深入,它必将会进一步为科学发展做出自己独特的贡献。 展开更多
关键词 Electron Movements pinned down to the Split Second
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Evaluation of temperature dependent vortex pinning properties in strongly pinned YBa_(2)Cu_(3)O_(7-δ)thin films with Y_(2)BaCuO_(5)nanoinclusions 被引量:1
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作者 Alok K.Jha Kaname Matsumoto +5 位作者 Tomoya Horide Shrikant Saini Ataru Ichinose Paolo Mele Yutaka Yoshida Satoshi Awaji 《Superconductivity》 2024年第1期95-105,共11页
The pinning of quantized magnetic vortices in superconducting YBa_(2)Cu_(3)O_(7-δ)(YBCO or Y123)thin films with Y_(2)BaCuO_(5)(Y211)nanoinclusions have been investigated over wide temperature range(4.2-77 K).The conc... The pinning of quantized magnetic vortices in superconducting YBa_(2)Cu_(3)O_(7-δ)(YBCO or Y123)thin films with Y_(2)BaCuO_(5)(Y211)nanoinclusions have been investigated over wide temperature range(4.2-77 K).The concentration of Y211 nanoinclusions has been systematically varied inside YBCO thin films prepared by laser ablation technique using surface modified target approach.Large pinning force density values(Fp∼0.5 TNm^(−3)at 4.2 K,9 T)have been observed for the YBCO film with moderate concentration of Y211 nanoinclusions(3.6 area%on ablation target).In addition,uniform enhancement in critical current density(J_(c))was observed in the angular dependent J_(c)measurement of YBCO+Y211 nanocomposite films.Y211 nanoinclusions have been found to be very efficient in pinning the quantized vortices thereby enhancing the in‐field J_(c)values over a wide range of temperature.Increasing the concentration of Y211 secondary phase into Y123 film matrix results into agglomeration of Y211 phase and observed as increased Y211 nanoparticle size.These larger secondary phase nanoparticles are not as efficient pinning centers at lower temperatures as they are at higher temperatures due to substantial reduction of the coherence length at lower temperatures.Investigation of the temperature dependence of J_(c)for YBCO+Y211 nanocomposite films has been conducted and possible vortex pinning mechanism in these nanocomposite films has been discussed. 展开更多
关键词 YBCO thin film Critical current Vortex pinning Nanoscale APCs
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大频率比的毫米波频率可重构滤波天线 被引量:1
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作者 杨国 施鸿强 +3 位作者 黎小聪 肖如奇 齐世山 吴文 《物理学报》 北大核心 2025年第1期270-280,共11页
针对现有毫米波频率可重构天线频率调谐比小的问题,本文设计了一款宽频带双频毫米波频率可重构滤波天线.天线以PIN二极管为重构开关,选择阶梯阻抗谐振器作为可重构带通滤波器,并加载U型枝节引入陷波;通过微带线-带状线过渡馈电结构将滤... 针对现有毫米波频率可重构天线频率调谐比小的问题,本文设计了一款宽频带双频毫米波频率可重构滤波天线.天线以PIN二极管为重构开关,选择阶梯阻抗谐振器作为可重构带通滤波器,并加载U型枝节引入陷波;通过微带线-带状线过渡馈电结构将滤波器与Vivaldi天线级联,并设计了金属腔隔离辐射和滤波两部分,有效降低了电磁干扰和交叉极化.测试结果显示:PIN二极管处于导通状态时,天线工作在25.9-28.6 GHz,最大增益为8.83 dBi;PIN二极管处于断开状态时,天线工作在32.6-35.9 GHz,最大增益为9.97 dBi;可重构中心频率比达到了1∶1.26,两个频带的交叉极化电平均低于-20 dB. 展开更多
关键词 毫米波天线 滤波天线 频率可重构 PIN二极管
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基于GaAs PIN二极管工艺的宽带单刀四掷开关芯片设计研究
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作者 白元亮 默立冬 +2 位作者 刘永强 朱思成 周鑫 《通讯世界》 2025年第4期13-15,共3页
采用GaAs PIN二极管工艺设计了一款2 GHz~18 GHz宽带单刀四掷开关芯片。采用串并联开关电路结构拓宽电路工作频段,并利用LC宽带偏置电路网络对开关电路进行加电,开关芯片尺寸为2.8 mm×2.8 mm×0.07 mm。经测试,该开关芯片在2 G... 采用GaAs PIN二极管工艺设计了一款2 GHz~18 GHz宽带单刀四掷开关芯片。采用串并联开关电路结构拓宽电路工作频段,并利用LC宽带偏置电路网络对开关电路进行加电,开关芯片尺寸为2.8 mm×2.8 mm×0.07 mm。经测试,该开关芯片在2 GHz~18 GHz频带内,控制端施加-5 V/0 V电压组合时,其插入损耗小于1.0 dB,隔离度大于32 dB,幅度一致性为-1~3 dB,相位一致性为±4°。 展开更多
关键词 GaAs PIN二极管 宽带 单刀四掷开关芯片 串并联 LC宽带偏置电路网络
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基于GaN的可重构宽带Doherty功放设计 被引量:1
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作者 赵国华 陈振中 +1 位作者 陈益 刘太君 《微波学报》 北大核心 2025年第1期1-5,共5页
传统Doherty功率放大器的带宽受限于λ/4传输线在不同频率处产生的相位偏移。本文设计了一种基于氮化镓的可重构宽带Doherty功率放大器(DPA),利用PIN开关实现峰值功率放大器支路的切换,相对减小了λ/4相位补偿线产生的相移范围,从而有... 传统Doherty功率放大器的带宽受限于λ/4传输线在不同频率处产生的相位偏移。本文设计了一种基于氮化镓的可重构宽带Doherty功率放大器(DPA),利用PIN开关实现峰值功率放大器支路的切换,相对减小了λ/4相位补偿线产生的相移范围,从而有效地提高了DPA的带宽。此外,在峰值功率放大器支路的输出匹配网络里还引入了谐波抑制网络来提高DPA的效率。为了验证设计有效性,使用氮化镓高电子迁移率晶体管CGH40010F设计并加工完成了一款宽带高效率DPA。测试结果表明,在2.8 GHz~3.6 GHz频段内,饱和输出功率为42 dBm~44.7 dBm,饱和漏极效率为59.4%~69%,功率回退6 dB时漏极效率在41.3%~48.8%。 展开更多
关键词 DOHERTY功率放大器 可重构 PIN开关 宽带 高效率
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基于PIN二极管的兆瓦级快速倒相开关设计
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作者 陈良萍 殷勇 +6 位作者 江涛 秦雨 李海龙 王彬 毕亮杰 熊正锋 蒙林 《强激光与粒子束》 北大核心 2025年第9期20-26,共7页
为研究基于磁控管等电真空振荡器的低成本、小型化、稳定且可阵列化应用的SLAC能量倍增器(SLED),设计了一种功率容量兆瓦级、响应时间纳秒级的高功率快速倒相开关。在波导结构中插入传统PIN二极管加载线型移相电路单元,通过波导外置偏... 为研究基于磁控管等电真空振荡器的低成本、小型化、稳定且可阵列化应用的SLAC能量倍增器(SLED),设计了一种功率容量兆瓦级、响应时间纳秒级的高功率快速倒相开关。在波导结构中插入传统PIN二极管加载线型移相电路单元,通过波导外置偏置电路控制PIN二极管的“开”/“关”状态,改变移相电路的等效阻抗以控制波导传输微波相位。已通过高功率实验验证了此类二极管波导移相器的高功率特性。通过级联8个移相电路单元实现180°相移。对所设计的倒相开关进行了频域与时域参数测试:频域测试结果表明,该倒相开关在工作频率下的插损小于0.7 dB,在中心频率2.458 GHz处相移172°,相移量与仿真设计值相比误差在±4°以内;时域测试结果表明,该倒相开关的倒相时间约为5 ns。 展开更多
关键词 能量倍增器 脉冲压缩器 倒相开关 PIN二极管
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鹅掌楸3个LcPIN1同源基因在体胚发生中的表达模式和功能分析
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作者 郝兆东 马筱筱 +5 位作者 衡柳宏 陆叶 鲁路 马营轩 施季森 陈金慧 《南京林业大学学报(自然科学版)》 北大核心 2025年第4期57-70,共14页
【目的】解析鹅掌楸(Liriodendron chinense)生长素转运蛋白PIN1在体胚发生中的表达模式与功能。【方法】通过qRT-PCR初步明确鹅掌楸3个LcPIN1同源基因(LcPIN1a、LcPIN1b和LcPIN1c)在体胚发生中的时序表达模式。进一步通过克隆LcPIN1s... 【目的】解析鹅掌楸(Liriodendron chinense)生长素转运蛋白PIN1在体胚发生中的表达模式与功能。【方法】通过qRT-PCR初步明确鹅掌楸3个LcPIN1同源基因(LcPIN1a、LcPIN1b和LcPIN1c)在体胚发生中的时序表达模式。进一步通过克隆LcPIN1s启动子,分别驱动GUS报告基因和mCherry荧光报告基因,利用农杆菌介导的遗传转化方法转化杂交鹅掌楸胚性愈伤组织,获得转基因阳性愈伤后进行体胚发生,通过GUS染色和荧光观察,明确LcPIN1s在体胚发生中的时空表达模式。最后,通过克隆LcPIN1s编码序列并构建以CaMV35S启动子驱动的过表达载体,转化杂交鹅掌楸胚性愈伤并进行体胚发生,通过对体胚发生效率的统计分析,解析LcPIN1s在体胚发生中的功能。【结果】表达模式分析显示,LcPIN1同源基因在体胚发生过程中既存在时空上的冗余,也存在特定组织部位的特异性表达。其中,LcPIN1a和LcPIN1c在球形胚时期已经表现出极性表达模式,可能为后续的体胚顶基轴建立和器官分化奠定基础。在心形胚时期,3个同源基因表现出显著的表达分化,LcPIN1a特异定位于维管组织;LcPIN1b呈胚体均匀表达;而LcPIN1c则特异表达于茎尖和子叶原基部位。随着体胚发育进入鱼雷胚和子叶胚时期,3者的表达模式又趋于一致,从子叶顶端沿着维管组织一直延伸到胚根区域,表明其可能共同作用于胚体生长素浓度梯度的维持。过表达试验显示,LcPIN1基因的过表达均会导致体胚发生效率的降低,并且过表达倍数越高,体胚发生效率越低。【结论】在鹅掌楸体胚发生过程中,PIN1蛋白的时空精确性表达对于正常的体胚诱导和形态建成具有重要作用,过表达可能破坏生长素梯度稳态,不利于体胚发生。 展开更多
关键词 鹅掌楸 生长素转运蛋白 PIN1 表达模式 体胚发生
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