OBJECTIVE:To observe the effect of Pingan Fang(PG)on behavioral sensitization and conditioned place preference(CPP)induced by ethanol in mice,and to determine the intervention mechanism of PG on alcohol addiction.METH...OBJECTIVE:To observe the effect of Pingan Fang(PG)on behavioral sensitization and conditioned place preference(CPP)induced by ethanol in mice,and to determine the intervention mechanism of PG on alcohol addiction.METHODS:A behavioral sensitization mouse model induced by ethanol was established to observe the effect of PG on the development and expression of behavioral sensitization induced by ethanol by recording the spontaneous activity of mice.The resident time of mice in a white box was measured to evaluate the effect of PG on developing CPP induced by ethanol.Concentrations of dopamine(DA),Glutamate(Glu),and?-aminobutyric acid(GABA)in the corresponding mesolimbic region of mice were determined by enzyme-linked immunosorbent assay.RESULTS:Although PG did not alter spontaneous activity in mice,it reduced the growth of spontaneous activity stimulated by ethanol.The residence time in the white box after-ethanol-training of mice in CPP experiments was decreased.CONCLUSION:Our data suggested that PG blocked the development and expression of behavioral sensitization induced by ethanol and the development of CPP in mice.The mechanism might be related to the decreased content of DA and Glu and increased content of GABA in the mesolimbic dopamine system.This suggests that PG might be useful for the prevention and treatment of alcohol addiction.展开更多
Objective: to analyze the clinical effect of Yangxue Pinggan Decoction on hypertensive insomnia. Methods: a total of 100 patients with hypertensive insomnia participated in the study. The patients were admitted to our...Objective: to analyze the clinical effect of Yangxue Pinggan Decoction on hypertensive insomnia. Methods: a total of 100 patients with hypertensive insomnia participated in the study. The patients were admitted to our hospital from January 2019 to January 2020. After they were randomly divided into two groups, the control group was treated with western medicine, and the observation group was treated with Yangxue Pinggan Decoction. The efficacy, sleep conditions and blood treatment of the two groups were compared. Results: the effective rate of observation group was 96% (48 cases) higher than that of control group with significant difference between groups (P < 0.05). The PSQI and SPIEGEL scores in the observation group after treatment were lower than those in the control group (10.01±0.32 and 10.12±0.34)points, respectively, and the differences were significant between the groups (P < 0.05). The systolic and diastolic blood pressure in the observation group after treatment were lower than those in the control group, being (95.32±3.28)mmHg, (76.78±3.74)mmHg, respectively, and the differences were significant between the groups (P < 0.05). Conclusion: Yangxue Pinggan Decoction has significant effect on hypertensive insomnia and can improve blood pressure of patients, so it is worthy of use.展开更多
基金Supported by Grants from The Administration of Traditional Chinese Medicine Sichuan Province(Exploration of Molecular Mechanisms of Fuyangfang Which Based on the TCM Theory of Yang Deficiency Qi Qie about Alcohol Addiction about Alcohol Addiction Memory and Eliminate,No.2014-E-069)
文摘OBJECTIVE:To observe the effect of Pingan Fang(PG)on behavioral sensitization and conditioned place preference(CPP)induced by ethanol in mice,and to determine the intervention mechanism of PG on alcohol addiction.METHODS:A behavioral sensitization mouse model induced by ethanol was established to observe the effect of PG on the development and expression of behavioral sensitization induced by ethanol by recording the spontaneous activity of mice.The resident time of mice in a white box was measured to evaluate the effect of PG on developing CPP induced by ethanol.Concentrations of dopamine(DA),Glutamate(Glu),and?-aminobutyric acid(GABA)in the corresponding mesolimbic region of mice were determined by enzyme-linked immunosorbent assay.RESULTS:Although PG did not alter spontaneous activity in mice,it reduced the growth of spontaneous activity stimulated by ethanol.The residence time in the white box after-ethanol-training of mice in CPP experiments was decreased.CONCLUSION:Our data suggested that PG blocked the development and expression of behavioral sensitization induced by ethanol and the development of CPP in mice.The mechanism might be related to the decreased content of DA and Glu and increased content of GABA in the mesolimbic dopamine system.This suggests that PG might be useful for the prevention and treatment of alcohol addiction.
文摘Objective: to analyze the clinical effect of Yangxue Pinggan Decoction on hypertensive insomnia. Methods: a total of 100 patients with hypertensive insomnia participated in the study. The patients were admitted to our hospital from January 2019 to January 2020. After they were randomly divided into two groups, the control group was treated with western medicine, and the observation group was treated with Yangxue Pinggan Decoction. The efficacy, sleep conditions and blood treatment of the two groups were compared. Results: the effective rate of observation group was 96% (48 cases) higher than that of control group with significant difference between groups (P < 0.05). The PSQI and SPIEGEL scores in the observation group after treatment were lower than those in the control group (10.01±0.32 and 10.12±0.34)points, respectively, and the differences were significant between the groups (P < 0.05). The systolic and diastolic blood pressure in the observation group after treatment were lower than those in the control group, being (95.32±3.28)mmHg, (76.78±3.74)mmHg, respectively, and the differences were significant between the groups (P < 0.05). Conclusion: Yangxue Pinggan Decoction has significant effect on hypertensive insomnia and can improve blood pressure of patients, so it is worthy of use.
