[Objective] The aim was to clone and express the pilA gene of outer membrane protein of Haemophilus parasuis.[Method] The published pilA gene sequence of HPS was analyzed for primer synthesis,and the genome of serotyp...[Objective] The aim was to clone and express the pilA gene of outer membrane protein of Haemophilus parasuis.[Method] The published pilA gene sequence of HPS was analyzed for primer synthesis,and the genome of serotype 5-type of HPS was used as template for PCR amplification of the pilA gene of HPS;the recombinant expression plasmid was constructed and transformed into E.coli BL21（DE3）after induced by IPTG.SDS-PAGE and Western blot analysis were then carried out.[Result] The molecular weight of expressed protein was consistent with the expected（43 kD）.[Conclusion] The results provided a foundation for the preparation of subunit vaccine and diagnostic reagents.展开更多
An international open access publishing pl atform based British Columbia of Canada,publishing scientific journals in the field of life science,now 38 journals have been launched,all are academic journals with professi...An international open access publishing pl atform based British Columbia of Canada,publishing scientific journals in the field of life science,now 38 journals have been launched,all are academic journals with professional peer review.As a member of Publishers International Linking Association,PILA,Bio Publisher follows the Creative Commons Attribution License,and all publications and articles published in Bio展开更多
http://biopublisher.caAn international open access publishing pl atform based British Columbia of Canada,publishing scientific journals in the field of life science,now 38 journals have been launched,all are academic ...http://biopublisher.caAn international open access publishing pl atform based British Columbia of Canada,publishing scientific journals in the field of life science,now 38 journals have been launched,all are academic journals with professional peer review.As a member of Publishers International Linking Association,PILA,Bio Publisher fol-展开更多
基金Supported by National Natural Science Foundation of China(31001072)National High Technology Research and Development Program of China(2006AA10A206)+1 种基金Youth Foundation of Beijing Academy of Agriculture and Forestry(QNJJ201012)Program of Beijing Academy of Agriculture and Forestry(2010A008)~~
文摘[Objective] The aim was to clone and express the pilA gene of outer membrane protein of Haemophilus parasuis.[Method] The published pilA gene sequence of HPS was analyzed for primer synthesis,and the genome of serotype 5-type of HPS was used as template for PCR amplification of the pilA gene of HPS;the recombinant expression plasmid was constructed and transformed into E.coli BL21（DE3）after induced by IPTG.SDS-PAGE and Western blot analysis were then carried out.[Result] The molecular weight of expressed protein was consistent with the expected（43 kD）.[Conclusion] The results provided a foundation for the preparation of subunit vaccine and diagnostic reagents.
文摘An international open access publishing pl atform based British Columbia of Canada,publishing scientific journals in the field of life science,now 38 journals have been launched,all are academic journals with professional peer review.As a member of Publishers International Linking Association,PILA,Bio Publisher follows the Creative Commons Attribution License,and all publications and articles published in Bio
文摘http://biopublisher.caAn international open access publishing pl atform based British Columbia of Canada,publishing scientific journals in the field of life science,now 38 journals have been launched,all are academic journals with professional peer review.As a member of Publishers International Linking Association,PILA,Bio Publisher fol-
