Proteins without transmembrane domains could be anchored to the cell surface for regulating various biological processes when covalently linked to glycosylphosphatidylinositol(GPI)molecules by the GPI transamidase(GPI...Proteins without transmembrane domains could be anchored to the cell surface for regulating various biological processes when covalently linked to glycosylphosphatidylinositol(GPI)molecules by the GPI transamidase(GPIT)complex.However,it remains poorly understood whether and how the GPIT complex affects primordial germ cell(PGC)development.In this study,we report the important roles of the GPIT complex in PGC migration and development in zebrafish embryos.Mutation of pigu or pigk,both encoding essential GPIT complex subunits,resulted in defective PGC migration with ectopically located PGCs and reduction of PGC counts.Notably,a detailed analysis of filopodia in PGCs revealed the attenuated polarity of filopodia distribution along the migration direction in mutant embryos.PGC transplantation and PGC-specific rescue experiments demonstrated that both PGC and somatic cell-expressed Pigu are required for PGC migration.Furthermore,expression levels of PGC-specific genes decreased in pigu mutant PGCs with the derepression of somatic cell genes.Hence,we propose that the GPIT complex plays a critical role during PGC migration and development.展开更多
Background:Hypertension is one of the most common comorbid conditions of epilepsy.Hypertension and epilepsy may be related to each other.Qigong Bigu practice induces a similar effect as fasting in the first week.As ke...Background:Hypertension is one of the most common comorbid conditions of epilepsy.Hypertension and epilepsy may be related to each other.Qigong Bigu practice induces a similar effect as fasting in the first week.As ketogenesis is induced during ketogenic diet therapy,we hypothesize that ketogenesis is detectable and related body weight loss would occur during the first week of Qigong Bigu practice.Methods:During the prospective observational study,34 healthy adult participants attended the Qigong Bigu practice for one week.The blood pressure,body weight,calorie consumption,blood glucose and beta-hydroxybutyrate level were measured.Results:The body weight and body mass index decreased by 2.39±1.34 kg(95%CI 1.92–2.85)and 0.94±0.57(95%CI 0.73–1.15),respectively,after five days of practice(P<0.001).The systolic and diastolic blood pressure decreased by 17.86±14.17 mmHg(95%CI 12.36–23.35)and 9.75±7.45 mmHg(95%CI 6.86–12.64),respectively(P<0.001).The average five-day calorie consumption was 1197.47±569.97 kcal(95%CI 998.60–1396.35).Meanwhile,no symptomatic hypoglycemia or other significant side effects were observed.The blood beta-hydroxybutyrate level increased to a nutritional level of 1.15±1.12 mmol/L(95%CI 0.76–1.62).The calorie consumption negatively correlated to the beta-hydroxybutyrate level in the blood.The loss of body weight and the decrease of body mass index were positively correlated to the blood beta-hydroxybutyrate level.Conclusions:Qigong Bigu can decrease the blood pressure,the body weight and the body mass index in healthy adult participants.The fasting stage of Qigong Bigu is accompanied by ketogenesis.Clinical trial of Qigong Bigu in hypertension and epilepsy patients might be worthwhile.The blood beta-hydroxybutyrate might be used as a biomarker to evaluate the effect of Qigong Bigu practice during fasting.展开更多
Background:Hypertension is one of the most common comorbid conditions of epilepsy.Hypertension and epilepsy may be related to each other.Qigong Bigu practice induces a similar effect as fasting in the first week.As ke...Background:Hypertension is one of the most common comorbid conditions of epilepsy.Hypertension and epilepsy may be related to each other.Qigong Bigu practice induces a similar effect as fasting in the first week.As ketogenesis is induced during ketogenic diet therapy,we hypothesize that ketogenesis is detectable and related body weight loss would occur during the first week of Qigong Bigu practice.Methods:During the prospective observational study,34 healthy adult participants attended the Qigong Bigu practice for one week.The blood pressure,body weight,calorie consumption,blood glucose and beta-hydroxy butyrate level were measured.Results:The body weight and body mass index decreased by 2.39±1.34 kg(95%CI1.92-2.85)and 0.94±0.57(95%CI 0.73-1.15),respectively,after five days of practice(P<0.001).The systolic and diastolic blood pressure decreased by 17.86±14.17 mmHg(95%Ci 12.36-23.35)and 9.75±7.45 mmHg(95%CI 6.86-12.64),respectively(P<0.001).The average five-day calorie consumption was 1197.47±569.97 kcal(95%CI 998.60-1396.35).Meanwhile,no symptomatic hypoglycemia or other significant side effects were observed.The blood beta-hydroxy butyrate level increased to a nutritional level of 1.15±1.12 mmol/L(95%CI 0.76-1.62).The calorie consumption negatively correlated to the beta-hydroxy butyrate level in the blood.The loss of body weight and the decrease of body mass index were positively correlated to the blood beta-hydroxy butyrate level.Conclusions:Qigong Bigu can decrease the blood pressure,the body weight and the body mass index in healthy adult participants.The fasting stage of Qigong Bigu is accompanied by ketogenesis.Clinical trial of Qigong Bigu in hypertension and epilepsy patients might be worthwhile.The blood beta-hydroxy butyrate might be used as a biomarker to evaluate the effect of Qigong Bigu practice during fasting.展开更多
基金financially supported by grants from the National Natural Science Foundation of China(31988101 to A.M.)the National Key Research and Development Program of China(2023YFA1800300 to X.W.and 2018YFC1003304 to A.M.)Yunnan Provincial Science and Technology Project at Southwest United Graduate School(202302AO370011 to A.M.).
文摘Proteins without transmembrane domains could be anchored to the cell surface for regulating various biological processes when covalently linked to glycosylphosphatidylinositol(GPI)molecules by the GPI transamidase(GPIT)complex.However,it remains poorly understood whether and how the GPIT complex affects primordial germ cell(PGC)development.In this study,we report the important roles of the GPIT complex in PGC migration and development in zebrafish embryos.Mutation of pigu or pigk,both encoding essential GPIT complex subunits,resulted in defective PGC migration with ectopically located PGCs and reduction of PGC counts.Notably,a detailed analysis of filopodia in PGCs revealed the attenuated polarity of filopodia distribution along the migration direction in mutant embryos.PGC transplantation and PGC-specific rescue experiments demonstrated that both PGC and somatic cell-expressed Pigu are required for PGC migration.Furthermore,expression levels of PGC-specific genes decreased in pigu mutant PGCs with the derepression of somatic cell genes.Hence,we propose that the GPIT complex plays a critical role during PGC migration and development.
基金supported by Sanming Project of Medicine in Shenzhen(SZSM201812005)Shenzhen Key Medical Discipline Construction Fund(No.SZXK033)Shenzhen Fund for Guangdong Provincial High Level Clinical Key Specialties(No.SZGSP012).
文摘Background:Hypertension is one of the most common comorbid conditions of epilepsy.Hypertension and epilepsy may be related to each other.Qigong Bigu practice induces a similar effect as fasting in the first week.As ketogenesis is induced during ketogenic diet therapy,we hypothesize that ketogenesis is detectable and related body weight loss would occur during the first week of Qigong Bigu practice.Methods:During the prospective observational study,34 healthy adult participants attended the Qigong Bigu practice for one week.The blood pressure,body weight,calorie consumption,blood glucose and beta-hydroxybutyrate level were measured.Results:The body weight and body mass index decreased by 2.39±1.34 kg(95%CI 1.92–2.85)and 0.94±0.57(95%CI 0.73–1.15),respectively,after five days of practice(P<0.001).The systolic and diastolic blood pressure decreased by 17.86±14.17 mmHg(95%CI 12.36–23.35)and 9.75±7.45 mmHg(95%CI 6.86–12.64),respectively(P<0.001).The average five-day calorie consumption was 1197.47±569.97 kcal(95%CI 998.60–1396.35).Meanwhile,no symptomatic hypoglycemia or other significant side effects were observed.The blood beta-hydroxybutyrate level increased to a nutritional level of 1.15±1.12 mmol/L(95%CI 0.76–1.62).The calorie consumption negatively correlated to the beta-hydroxybutyrate level in the blood.The loss of body weight and the decrease of body mass index were positively correlated to the blood beta-hydroxybutyrate level.Conclusions:Qigong Bigu can decrease the blood pressure,the body weight and the body mass index in healthy adult participants.The fasting stage of Qigong Bigu is accompanied by ketogenesis.Clinical trial of Qigong Bigu in hypertension and epilepsy patients might be worthwhile.The blood beta-hydroxybutyrate might be used as a biomarker to evaluate the effect of Qigong Bigu practice during fasting.
基金This work was supported by Sanming Project of Medicine in Shenzhen(SZSM201812005)Shenzhen Key Medical Discipline Construction Fund(No.SZXK033)Shenzhen Fund for Guangdong Provincial High Level Clinical Key Specialties(No.SZGSP012).
文摘Background:Hypertension is one of the most common comorbid conditions of epilepsy.Hypertension and epilepsy may be related to each other.Qigong Bigu practice induces a similar effect as fasting in the first week.As ketogenesis is induced during ketogenic diet therapy,we hypothesize that ketogenesis is detectable and related body weight loss would occur during the first week of Qigong Bigu practice.Methods:During the prospective observational study,34 healthy adult participants attended the Qigong Bigu practice for one week.The blood pressure,body weight,calorie consumption,blood glucose and beta-hydroxy butyrate level were measured.Results:The body weight and body mass index decreased by 2.39±1.34 kg(95%CI1.92-2.85)and 0.94±0.57(95%CI 0.73-1.15),respectively,after five days of practice(P<0.001).The systolic and diastolic blood pressure decreased by 17.86±14.17 mmHg(95%Ci 12.36-23.35)and 9.75±7.45 mmHg(95%CI 6.86-12.64),respectively(P<0.001).The average five-day calorie consumption was 1197.47±569.97 kcal(95%CI 998.60-1396.35).Meanwhile,no symptomatic hypoglycemia or other significant side effects were observed.The blood beta-hydroxy butyrate level increased to a nutritional level of 1.15±1.12 mmol/L(95%CI 0.76-1.62).The calorie consumption negatively correlated to the beta-hydroxy butyrate level in the blood.The loss of body weight and the decrease of body mass index were positively correlated to the blood beta-hydroxy butyrate level.Conclusions:Qigong Bigu can decrease the blood pressure,the body weight and the body mass index in healthy adult participants.The fasting stage of Qigong Bigu is accompanied by ketogenesis.Clinical trial of Qigong Bigu in hypertension and epilepsy patients might be worthwhile.The blood beta-hydroxy butyrate might be used as a biomarker to evaluate the effect of Qigong Bigu practice during fasting.
