Global brain ischemia and neurological deficit are consequences of cardiac arrest that lead to high mortality.Despite advancements in resuscitation science,our limited understanding of the cellular and molecular mecha...Global brain ischemia and neurological deficit are consequences of cardiac arrest that lead to high mortality.Despite advancements in resuscitation science,our limited understanding of the cellular and molecular mechanisms underlying post-cardiac arrest brain injury have hindered the development of effective neuroprotective strategies.Previous studies primarily focused on neuronal death,potentially overlooking the contributions of non-neuronal cells and intercellular communication to the pathophysiology of cardiac arrest-induced brain injury.To address these gaps,we hypothesized that single-cell transcriptomic analysis could uncover previously unidentified cellular subpopulations,altered cell communication networks,and novel molecular mechanisms involved in post-cardiac arrest brain injury.In this study,we performed a single-cell transcriptomic analysis of the hippocampus from pigs with ventricular fibrillation-induced cardiac arrest at 6 and 24 hours following the return of spontaneous circulation,and from sham control pigs.Sequencing results revealed changes in the proportions of different cell types,suggesting post-arrest disruption in the blood-brain barrier and infiltration of neutrophils.These results were validated through western blotting,quantitative reverse transcription-polymerase chain reaction,and immunofluorescence staining.We also identified and validated a unique subcluster of activated microglia with high expression of S100A8,which increased over time following cardiac arrest.This subcluster simultaneously exhibited significant M1/M2 polarization and expressed key functional genes related to chemokines and interleukins.Additionally,we revealed the post-cardiac arrest dysfunction of oligodendrocytes and the differentiation of oligodendrocyte precursor cells into oligodendrocytes.Cell communication analysis identified enhanced post-cardiac arrest communication between neutrophils and microglia that was mediated by neutrophil-derived resistin,driving pro-inflammatory microglial polarization.Our findings provide a comprehensive single-cell map of the post-cardiac arrest hippocampus,offering potential novel targets for neuroprotection and repair following cardiac arrest.展开更多
Wind disturbance has emerged as a potential eco-friendly method for seedling cultivation.In this study,an electromechanical device was designed and built to investigate the effects of airflow on the micro-environment ...Wind disturbance has emerged as a potential eco-friendly method for seedling cultivation.In this study,an electromechanical device was designed and built to investigate the effects of airflow on the micro-environment and physiological activities of tomato seedlings in seedbeds by controlled experiments.The results indicated that airflow could enhance CO_(2) concentration near the seedling canopy,accelerate water evaporation from the seedling substrate,and reduce fluctuations in the temperature and humidity in microclimate.The photosynthetic rates of leaves at the 4th,7th,and 10th positions in seedlings subjected to airflow increased by 25.04%,8.23%,and 8.47%,respectively,whereas the transpiration rates increased by 15.59%,22.28%,and 13.26%,respectively when compared to the control group.Additionally,the strong seedling index of seedlings treated with airflow and exogenous iron element increased by 26.02%and 31.5%,respectively.Compared to seedlings treated with exogenous iron element,the geometric mean diameter of the pith tissue cells in the stems of seedlings subjected to airflow disturbance was reduced by approximately 18.66%,while the elastic modulus and bending strength of the stems increased by 10.01%and 5.89%,respectively.Similarly,the volume of root tissue cells decreased by 19.22%,but the elastic modulus of the roots increased by 6.46%.This study confirms that airflow significantly enhances seedling resilience to abiotic stress,yielding similar or better outcomes than exogenous iron application.It provides both theoretical and practical support for using airflow disturbance as a green technology for cultivating robust seedlings.展开更多
Objective:To investigate the spatial gradient of intraoperative impedance across the cochlear electrode array in pediatric cochlear implant recipients and assess its potential as a physiological indicator for the elec...Objective:To investigate the spatial gradient of intraoperative impedance across the cochlear electrode array in pediatric cochlear implant recipients and assess its potential as a physiological indicator for the electrode-neural interface.Methods:A prospective observational study involving 56 pediatric patients underwent cochlear implantation with Cochlear Nucleus devices.Intraoperative polarized impedance and electrically evoked compound action potential(ECAP)threshold were recorded across all 1232 electrodes using AutoNRT software.Eight electrodes with open-or short-circuit were excluded,leaving 1,224 for analysis.Impedance values were categorized by cochlear region(basal,middle,apical),and electrodes with elevated impedance(10-20 kΩ)were analyzed for regional distribution and clinical relevance.Data were analyzed for spatial patterns and correlation with the ECAP threshold profiles.Results:A consistent basal-to-apical increase in impedance was observed(7.7±1.9,9.2±1.4,10.8±1.5 kΩ;p<0.001).Impedance and ECAP threshold were weakly correlated(ρ=-0.20,p<0.001;β=-1.26,p<0.001),with a positive association in the apical region(ρ=0.12,p=0.048).Electrodes with higher impedance(1020 kΩ)were less likely to show elevated or absent TNRT(OR=0.175,p=0.02).The impedance gradient persisted across age groups and was significantly correlated with ECAP threshold patterns.Conclusion:Intraoperative impedance monitoring reveals a strong and physiologically consistent gradient,with higher values in apical electrodes.This gradient reflects anatomical and tissue interface variations,which may offer a valuable physiological indicator for intraoperative electrode positioning and neural interface integrity.展开更多
Foliar uptake of airborne metal(loid)s plays a crucial role in metal(loid)accumulation in plant organs and is influ-enced by the size and emission sources of aerosols.Given the high enrichment of toxic metal(loid)s in...Foliar uptake of airborne metal(loid)s plays a crucial role in metal(loid)accumulation in plant organs and is influ-enced by the size and emission sources of aerosols.Given the high enrichment of toxic metal(loid)s in submicron-scale particulates(PM1),this study established a PM1 exposure system to examine airborne metal(loid)accu-mulation and foliar physiological responses in Oryza sativa L.The results showed that the concentrations of Cu,Zn,As,Pb,and Cd in the leaves and grains were influenced not only by the airborne metal(loid)levels but also by the specific nature of the PM1 particles.The quantitative model for PM1-associated Pb entry into leaf tissue indicated that foliar Pb accumulation was primarily driven by particle adhesion,followed by hydrophilic pene-tration and trans-stomatal liquid film migration,accounting for 87%–89%of the total accumulation.The strong hygroscopicity and high Pb activity of PM1 emitted from waste incineration(WI)increased the Pb absorption coefficient via the hydrophilic and liquid film migration pathway.In contrast,the high hydrophobicity of PM1 from coal burning(CB)led to greater retention of Pb on leaf surfaces.Both foliar reactive oxygen metabolism and photosynthesis indices were sensitive to air pollution.Foliar metal(loid)accumulation and airborne PM1 concentration accounted for the variance in physiological responses in rice leaves.Our results also indicated that Pb was the key element in PM1 emissions from both coal burning(CB)and waste incineration(WI)responsible for significant physiological changes in rice leaves.展开更多
Accurate detection of driver fatigue is essential for improving road safety.This study investigates the effectiveness of using multimodal physiological signals for fatigue detection while incorporating uncertainty qua...Accurate detection of driver fatigue is essential for improving road safety.This study investigates the effectiveness of using multimodal physiological signals for fatigue detection while incorporating uncertainty quantification to enhance the reliability of predictions.Physiological signals,including Electrocardiogram(ECG),Galvanic Skin Response(GSR),and Electroencephalogram(EEG),were transformed into image representations and analyzed using pretrained deep neu-ral networks.The extracted features were classified through a feedforward neural network,and prediction reliability was assessed using uncertainty quantification techniques such as Monte Carlo Dropout(MCD),model ensembles,and combined approaches.Evaluation metrics included standard measures(sensitivity,specificity,precision,and accuracy)along with uncertainty-aware metrics such as uncertainty sensitivity and uncertainty precision.Across all evaluations,ECG-based models consistently demonstrated strong performance.The findings indicate that combining multimodal physi-ological signals,Transfer Learning(TL),and uncertainty quantification can significantly improve both the accuracy and trustworthiness of fatigue detection systems.This approach supports the development of more reliable driver assistance technologies aimed at preventing fatigue-related accidents.展开更多
Intercropping has been widely used in arid and semi-arid regions because of its high yield,stable productivity,and efficient utilization of resources.However,in recent years,the high yield of traditional intercropping...Intercropping has been widely used in arid and semi-arid regions because of its high yield,stable productivity,and efficient utilization of resources.However,in recent years,the high yield of traditional intercropping is mainly attributed to the large amount of purchased resources such as water and fertilizer,plastic film,and mechanical power.These lead to a decline in cultivated land quality and exacerbate intercrops'premature root and canopy senescence.So,the application of traditional intercropping faces major challenges in crop production.This paper analyzes the manifestations,occurrence mechanisms,and agronomic regulatory pathways of crop senescence.The physiological and ecological characteristics of intercropping to delay root and canopy senescence of crops are reviewed in this paper.The main agronomic regulatory pathways of intercropping to delay root and canopy senescence of crops are based on above-and blow-ground interactions,including collocation of crop varieties,spatial arrangement,water and fertilizer management,and tillage and mulch practices.Future research fields of intercropping to delay root and canopy senescence should focus on the aspects of selecting and breeding special varieties,application of molecular biology techniques,and developing or applying models to predict and evaluate the root and canopy senescence process of intercrops.Comprehensive analysis and evaluation of different research results could provide a basis for enhancing intercropping delay root and canopy senescence through adopting innovative technologies for regulating the physio-ecological characteristics of intercrops.This would support developing and adopting high-yield,efficient,and sustainable intercropping systems in arid and semi-arid areas with high population density,limited land,and abundant light and heat resources.展开更多
Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negate...Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negates the advantages of large panicle and constrains yield potential.Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration,with the sensitive period for this process commencing approximately 15‒20 d before panicle heading.Notable positional variations occur within the panicle,with significantly higher spikelet degeneration rates at the basal than at the upper positions.An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress.Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport,which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation.Sucrose serves as the main carbohydrate source for spikelet development,demonstrating an apical dominance pattern that favors spikelet formation.However,under abiotic stress,the inhibition of sucrose decomposition,rather than sucrose transport impairment,predominantly contributes to aggravated spikelet degeneration at the basal panicle positions.Brassinolide and auxin have a significant relationship with spikelet formation,potentially mediating apical dominance.Specifically,brassinolide enhances sucrose accumulation and utilization,thereby alleviating spikelet degeneration.At present,the mechanisms underlying rice spikelet degeneration have not been fully revealed,and the joint effects of hormones,carbohydrates,and carbon and nitrogen metabolism on this process require further investigation.To reduce the spikelet degeneration,the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’resilience to abiotic stress.An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.展开更多
Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In...Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In this work,we investigated the effect of corrosion product film layers on the degradation behavior of pure Mg in physiological environments.Pure Mg shows a faster corrosion rate in simulated body fluid(SBF)compared to NaCl solution.Hydrogen evolution experiments indicate that the degradation rate of pure Mg in SBF decreases rapidly within the first 12 h but stabilizes afterward.The rapid deposition of low-solubility calcium phosphate on the pure Mg in SBF provides protection to the substrate,resulting in a gradual decrease in the degradation rates.Consequently,the corrosion product film of pure Mg formed in SBF exhibits a layered structure,with the upper layer consisting of dense Ca_(3)(PO_(4))_(2)/Mg_(3)(PO_(4))_(2) and the lower layer consisting of Mg(OH)_(2)/MgO.Electrochemical impedance spectroscopy(EIS)shows that the resistance of the corrosion product film increases over time,indicating gradual strengthening of the corrosion resistance.The 4-week degradation results in the femoral marrow cavity of mice are consistent with the result in SBF in vitro.展开更多
Drought is one of the most severe environmental stresses affecting soybean growth and development,especially in arid and semi-arid areas.The aim of this experiment is to evaluate the effect of regulated deficit irriga...Drought is one of the most severe environmental stresses affecting soybean growth and development,especially in arid and semi-arid areas.The aim of this experiment is to evaluate the effect of regulated deficit irrigation during the vegetative stages on soybean plants and determine the amount irrigation water can be reduced without affecting the physiological parameters,the crop phenology,and the yield of the soybean crop.The field experiments were conducted during two irrigation crop seasons(2021 and 2022)in Louata,Morocco.The results showed that regulated deficit irrigation regimes during the vegetative stages was combined with high temperatures and low air humidities during the beginning of flowering and the pod filling stage during 2021 in comparison with 2022,especially for 25%CWR(crop water requirements).Regulated deficit irrigation regimes reduced the stomatal conductance by 46%and 52%respectively during the first and second growing seasons by limiting CO_(2) intake for the Calvin cycle.The stomata closure increased the leaf temperature and affected the functioning of the photosynthetic apparatus by damaging the chlorophyll pigments and impairment of electron transport chains in chloroplasts.The transition from regulated deficit irrigation to 100%CWR at the beginning of flowering(R1)compensated for the photosynthetic loss,improved the growth and development of soybean plants and enhanced the yield and its components for 50%and 75%CWR.The adaptative mechanism such as the remobilization of the carbon reserved in the stems and leaves(vegetative tissues)to the grains improved the grain yield by 36.7%during 2021 and by 32.2%during 2022 and.This consequently improved the water use efficiency,the water productivity of soybean for 50%and 75%CWR and contributed to water saving with an average of 60 mm per growing season.展开更多
EHL-2 is a compact,high-field spherical tokamak designed to explore the potential of an advanced p-11B nuclear fusion reactor.Due to its high plasma current and thermal energy,it is crucial to mitigate the impact asso...EHL-2 is a compact,high-field spherical tokamak designed to explore the potential of an advanced p-11B nuclear fusion reactor.Due to its high plasma current and thermal energy,it is crucial to mitigate the impact associated with disruptions to ensure the safe operation of EHL-2.This paper evaluates the performance requirements of the disruption prediction system on EHL-2,with a particular focus on applying generalizable knowledge transfer from existing devices to future ones.Furthermore,the key characteristics of disruption mitigation strategies are analyzed,and their overall mitigation performance on EHL-2 is assessed.This insight provides valuable guidance for optimizing the engineering design of EHL-2 and identifying its optimal operational regime.展开更多
Hypnosis is a promising tool in the management of various conditions,such as anxiety and chronic pain.Preliminary studies have shown that hypnosis can directly affect the cardiovascular system,as it increases parasymp...Hypnosis is a promising tool in the management of various conditions,such as anxiety and chronic pain.Preliminary studies have shown that hypnosis can directly affect the cardiovascular system,as it increases parasympathetic activation and reduces sympathetic activity.However,the literature related to the effects of hypnosis on cardiovascular health is scarce,mainly due to misconceptions about hypnosis among researchers and medical professionals.This opinion paper examines the role that hypnosis may play in cardiovascular health,highlighting the physiological mechanisms behind it.The evidence suggests that hypnosis has both direct(e.g.,changes in the activity of the autonomic nervous system)and indirect(e.g.,changes in healthy behaviours)effects on the cardiovascular system;however,further studies are needed to properly define its mechanisms of action and its applicability in improving cardiovascular health.Thus,this opinion paper advocates the adoption of the term“hypno-cardiac physiology”to identify a new research area that gathers experts from neuroscience and cardiovascular science with the joint aim of seeking further understanding of the effects of hypnosis on the cardiovascular system.The adoption of a dedicated term to identify the study of the cardiovascular response to hypnosis will encourage its implementation in cardiovascular health interventions,promoting awareness of its effects among the public and the healthcare community,and promoting the formation of dedicated multidisciplinary research groups and dedicated educational training for healthcare professional interested in its applications.展开更多
Numerous bird species have adapted to rapid urbanization,navigating challenges introduced by novel stressors.While prior research has explored how urban exploiters adjust their physiology relative to their rural count...Numerous bird species have adapted to rapid urbanization,navigating challenges introduced by novel stressors.While prior research has explored how urban exploiters adjust their physiology relative to their rural counterparts during breeding season,the modulation of multiple physiological markers outside of reproduction,and the maintenance of sex-specific differences,remain less understood.Using an urban exploiter,the Eurasian tree sparrow(ETS;Passer montanus),we investigated how both sexes of urban and rural populations modulate twenty-two physiological markers of nutrition,body condition,oxidative stress,and antioxidative responses during pre-breeding stage.Our results showed that urban ETSs displayed distinct glucolipid metabolism compared to rural ETSs,characterized by higher levels of free fatty acids,β-hydroxybutyric acid,high-density lipoprotein(HDL),and total cholesterol,alongside lower levels of triglycerides,glucose(Glu),and fat content(FC).No significant differences were observed in other physiological markers.Additionally,both sexes of urban ETSs exhibited similar profiles across these markers,unlike rural populations where females showed higher Glu and total protein levels and lower HDL and FC levels than males.Our findings suggest that birds adjust their nutritional physiological markers in response to urbanization,likely due to dietary differences between urban and rural environments.Furthermore,sex differences in physiological markers of nutrition,body condition,oxidative stress,and antioxidant responses appear to diminish in urban settings.展开更多
Vascular disrupting agents(VDAs)can destroy tumor vasculature and lead to tumor ischemia and hypoxia,resulting in tumor necrosis.However,VDAs are easy to induce the upregulation of genes that are associated with cance...Vascular disrupting agents(VDAs)can destroy tumor vasculature and lead to tumor ischemia and hypoxia,resulting in tumor necrosis.However,VDAs are easy to induce the upregulation of genes that are associated with cancer cell drug resistance and angiogenesis in tumor cells.Hypoxia-activated chemotherapy will be an ideal supplement to VDAs therapy since it can help to fully utilize the ischemia and hypoxia induced by VDAs to realize a synergistic antitumor therapeutic outcome.Here,we design a liposome whose surface is modified with a tumor-homing peptide Cys-Arg-Glu-Lys-Ala(CREKA,which can specifically target tumor vessels and stroma)and whose aqueous cavity and lipid bilayer are loaded by a hypoxia-activatable drug banoxantrone dihydrochloride(AQ4N)and a VDA combretastatin A4(CA4),respectively.CA4 can selectively target vascular endothelial cells and destroy the tumor blood vessels,which will cause the rapid inhibition of blood flow in tumor and enhance the hypoxia in the tumor region.As a consequence,AQ4N can exert its boosted cytotoxicity under the enhanced hypoxic environment.The as-prepared liposome with a uniform particle size exhibits good stability and high cancer cell killing efficacy in vitro.In addition,in vivo experiments confirm the excellent tumortargeting/accumulation,tumor vasculature-damaging,and tumor inhibition effects of the liposome.This work develops a liposomal which can achieve safe and effective tumor suppression without external stimulus excitation by only single injection,and is expected to benefit the future development of effective antitumor liposomal drugs.展开更多
Efficient siRNA delivery is highly desirable for disease treatment.However,the application of conventional nanoparticles is limited by the inability to escape from endo-lysosomes.Herein,we report a strategy using smal...Efficient siRNA delivery is highly desirable for disease treatment.However,the application of conventional nanoparticles is limited by the inability to escape from endo-lysosomes.Herein,we report a strategy using small-molecule drugs to enhance siRNA endo-lysosomal release,addressing this challenge.We encapsulated gentamicin(GM)into the marketed Onpattro■ formulation to establish LNP-siRNA/GM nanoparticles that promote siRNA endo-lysosomal escape through endosomal disruption,mechanistically exhibiting unique functionality and synergistic effects of LNP-siRNA/GM to improve cancer therapy.Besides,GM induced reactive oxygen species(ROS)and phospholipids accumulation in endolysosomes,as well as the physical characteristics of lipid nanoparticles(LNPs)were preserved.We also revealed that GM causes endo-lysosomal swelling and disrupts the endosomal membrane to enable siRNA release,as confirmed by Galectin 3 recruitment and acridine orange release.This approach achieved∼81%mRNA-EGFR silencing,which is more than LNP-siEGFR(∼56.23%)by enhancing siRNA endo-lysosomal escape efficiency.Meanwhile,LNP-siEGFR/GM exhibited significant biological activities in HepG2 cells,driven by the synergistic effects of siEGFR and GM with the VEGF and CXCL12 downregulation of,and ROS and phospholipids upregulation.Furthermore,tumor growth was notably suppressed after intravenous injection of LNP-siEGFR/GM in tumor-bearing nude mice.The combination of EGFR-siRNA and GM could also greatly inhibit angiogenesis,be antiproliferative,and induce tumor cells apoptosis.Therefore,this GM and siRNA co-delivery system would provide an efficient strategy for siRNA endosomal escape,significantly improving knockdown in various LNPs based siRNA delivery systems and efficiently enhancing cancer therapy.展开更多
Airport disruptions often pose challenges in assigning aircraft to gates,resulting in infeasible planned schedules.In particular,a large number of transfer passengers miss their connections in the context of disruptio...Airport disruptions often pose challenges in assigning aircraft to gates,resulting in infeasible planned schedules.In particular,a large number of transfer passengers miss their connections in the context of disruptions,which cause huge economic losses to airlines and serious passengers’dissatisfaction.This paper proposes a set-partitioning-based model to optimize Aircraft-Gate Reassignment with Transfer Passenger Connections(AGRP-TPC),which incorporates flexible gate-swap and aircraft-delay operations to mitigate the overall impact of disruptions.To efficiently solve the model,we introduce the concepts of additive-transfer and nonstop-transfer to handle passenger connections,and develop a Hierarchical Column-and-Row Generation(HCRG)approach guided by airport terminal space attribute.The column generation and row generation procedures solve iteratively until no new variables and constraints are generated.In addition,a follow-on strategy and a diving heuristic are designed to efficiently obtain high-quality solutions.We evaluate the proposed approach using various instances from a major Chinese international airport.Computational results demonstrate that our approach outperforms the comparison algorithms and produces good solutions within the time limit.Detailed results indicate that our approach effectively reduces overall losses in aircraft-gate reassignment following disruptions,and it can serve as an auxiliary decision-making tool for airport operators and airlines.展开更多
Biofeedback is a behavioral intervention technology based on psychophysiology,which uses various electronic devices to collect the physiological activities of individuals in real time and provides them to themselves t...Biofeedback is a behavioral intervention technology based on psychophysiology,which uses various electronic devices to collect the physiological activities of individuals in real time and provides them to themselves through various ways,so that people can actively change their own physiological changes when they perceive the changes in some physiological functions of their own,and consciously control the physiological functions of various aspects of the autonomic nervous system within a fixed range.It can play a role in improving physical health status,preventing and treating some somatic diseases.Studies have shown that biofeedback relaxation training can make the training subjects learn to regulate their own physiological functions and psychological activities,improve negative emotional states such as depression and anxiety by regulating the body function,and can also be used to assist in the treatment of other clinical diseases.As a new psychological intervention,biofeedback relaxation training has great development potential and can be better applied to the prevention and treatment of physical and mental diseases in the general population in the future.展开更多
Morpho-physiological evaluation of a crop’s genetic resources is necessary to find possible genotypes to include in breeding initiatives.The objective of this study was to identify heat-tolerant wheat genotypes among...Morpho-physiological evaluation of a crop’s genetic resources is necessary to find possible genotypes to include in breeding initiatives.The objective of this study was to identify heat-tolerant wheat genotypes among 36 mutant lines using morpho-physiological indices.Seedlings of mutant lines and check varieties were grown under both normal(control)and heat-stress conditions in growth chambers.Data were recorded on root-shoot parameters(length,fresh weight,dry weight,and ratio),relative water content(RWC),stability of cell membrane,pigment content,and chlorophyll fluorescence.Two-way analysis of variance showed significant(p<0.01,p<0.001)variation among 15 morpho-physiological features in both growing conditions.Correlation studies showed that pigment-specific indices(total chl,chl a,chl b,carotenoid,Fv/Fm—chlorophyll fluorescence)had significant positive correlations with rootshoot fresh weight(RFW,SFW)and root-shoot dry weight(RDW,SDW),and negative association with relative water content,stability of cell membrane at both conditions.Hierarchical cluster analysis revealed three primary clusters where cluster III(18 genotypes)showed minimal changes in studied characters than cluster I(11 genotypes)and cluster II(13 genotypes).Results obtained from this study revealed that the identified heat-sensitive(30-2 SSH)and heat-tolerant genotypes(27-2(4),29-2(2),and 30-2 SSH)of wheat might provide useful breeding materials for enhancing wheat yield even more in this period of uncertain climate change.展开更多
The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness...The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness in the rehabilitation therapy of forearm muscles,due to the lack of the physiological characteristics of muscle activation and sequences.An armband with eight channels was used to collect surface electromyographic signals(sEMGs)of a specific section of the forearm under the different wrist movements,palm postures,and external loads,based on the image of magnetic resonance imaging(MRI).The collected cross-sectional muscles covered almost all surface muscles.The muscle activation could be expressed clearly by enveloping the sEMG signals of 8 muscles within a single cycle.The root mean square(RMS)and the average peak value V_(P) were used to evaluate the activation intensities of dominant muscles.The activation sequences and the absolute times of dominant muscles were obtained from the envelopes of their raw sEMGs,and not influenced by the palm postures and external loads.In addition,their RMS and V_(P) under each wrist movement increased approximate linearly with external loads.The corresponding contribution ratios were first calculated to evaluate the role played by each muscle.The well-defined data of forearm muscles could provide standard references for the rehabilitation therapy of forearm muscles.展开更多
Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A random...Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A randomized control trial parallel group design was conducted among 45 comatose patients divided into two intervention groups,i.e.nurse voice stimulus group,receiving nurses voice with standard care,family members voice stimulus group receiving their beloved family member voice with standard care and one control group receiving only standard care in medicine intensive care unit.The intervention was provided three times a day,each lasting 5 min for 7 days in addition to standard care.Repeated measure analysis of variance and independent t-test were used to compare within and between groups,respectively.Results:The study found significant differences in Glasgow coma scale(GCS)scores within both the nurse(F=2.78,P=0.042)and family member(F=10.27,P=0.0001)voice stimulus groups over 7 days.Comparing GCS scores between intervention groups showed significant variations before(P=0.028),during(P=0.047),and after(P=0.036)the intervention on day 7.Comparing GCS scores between the family members’voice stimulus group and the control group,significant changes were observed on days 5 and 7(P=0.043,0.030,0.030,and 0.014,0.012,0.012)before,during,and after the intervention.Conclusions:The use of beloved family members’voices proved more effective in elevating the patients’level of consciousness compared to both the nurse voice stimulus group and the control group.展开更多
Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives wer...Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives were sampled at the vegetative growth,floral bud differentiation,and bud emergence stages.No bolting was observed in bolting-resistant‘WA’on the 130th day after planting,whereas the bolting reached 39.22%in bolting-prone‘BA’,which was significantly higher than that of‘WA’.The contents of gibberellins,abscisic acid,and zeatin riboside after floral bud differentiation in‘WA’were significantly less than in‘BA’,whereas the indoleacetic acid content in‘WA’was significantly higher than that in‘BA’before and after floral bud differentiation.The soluble sugar content and nitrate reductase activity in‘BA’were significantly higher than those in‘WA’before and during floral bud differentiation periods.However,they were significantly lower in‘BA’compared with in‘WA’after bolting due to the nutrient consumption required by reproductive growth.A transcriptome analysis determined that the differentially expressed genes related to bolting tolerance were enriched in the terms‘photoperiodism,flowering’,‘auxin-activated signaling pathway’,‘gibberellic acid mediated signaling pathway’,and‘carbohydrate metabolic process’,and this was generally consistent with the physiological data.Additionally,12 key differentially expressed genes(including isoform_203018,isoform_481005,isoform_716975,and isoform_564877)related to bolting tolerance were investigated.This research provides new information for breeding bolting-tolerant chives.展开更多
基金supported by the National Science Foundation of China,Nos.82325031(to FX),82030059(to YC),82102290(to YG),U23A20485(to YC)Noncommunicable Chronic Diseases-National Science and Technology Major Project,No.2023ZD0505504(to FX),2023ZD0505500(to YC)the Key R&D Program of Shandong Province,No.2022ZLGX03(to YC).
文摘Global brain ischemia and neurological deficit are consequences of cardiac arrest that lead to high mortality.Despite advancements in resuscitation science,our limited understanding of the cellular and molecular mechanisms underlying post-cardiac arrest brain injury have hindered the development of effective neuroprotective strategies.Previous studies primarily focused on neuronal death,potentially overlooking the contributions of non-neuronal cells and intercellular communication to the pathophysiology of cardiac arrest-induced brain injury.To address these gaps,we hypothesized that single-cell transcriptomic analysis could uncover previously unidentified cellular subpopulations,altered cell communication networks,and novel molecular mechanisms involved in post-cardiac arrest brain injury.In this study,we performed a single-cell transcriptomic analysis of the hippocampus from pigs with ventricular fibrillation-induced cardiac arrest at 6 and 24 hours following the return of spontaneous circulation,and from sham control pigs.Sequencing results revealed changes in the proportions of different cell types,suggesting post-arrest disruption in the blood-brain barrier and infiltration of neutrophils.These results were validated through western blotting,quantitative reverse transcription-polymerase chain reaction,and immunofluorescence staining.We also identified and validated a unique subcluster of activated microglia with high expression of S100A8,which increased over time following cardiac arrest.This subcluster simultaneously exhibited significant M1/M2 polarization and expressed key functional genes related to chemokines and interleukins.Additionally,we revealed the post-cardiac arrest dysfunction of oligodendrocytes and the differentiation of oligodendrocyte precursor cells into oligodendrocytes.Cell communication analysis identified enhanced post-cardiac arrest communication between neutrophils and microglia that was mediated by neutrophil-derived resistin,driving pro-inflammatory microglial polarization.Our findings provide a comprehensive single-cell map of the post-cardiac arrest hippocampus,offering potential novel targets for neuroprotection and repair following cardiac arrest.
基金supported by an International Cooperation Key Plan of Shaanxi Province(Grant No.2022KWZ-12)an Agricultural Science Innovation and Transformation Project of Shaanxi Province[Grant No.NYKJ-2022-YL(XN)12]a High-End Foreign Expert Recruitment Program(Grant No.G2022172006L).
文摘Wind disturbance has emerged as a potential eco-friendly method for seedling cultivation.In this study,an electromechanical device was designed and built to investigate the effects of airflow on the micro-environment and physiological activities of tomato seedlings in seedbeds by controlled experiments.The results indicated that airflow could enhance CO_(2) concentration near the seedling canopy,accelerate water evaporation from the seedling substrate,and reduce fluctuations in the temperature and humidity in microclimate.The photosynthetic rates of leaves at the 4th,7th,and 10th positions in seedlings subjected to airflow increased by 25.04%,8.23%,and 8.47%,respectively,whereas the transpiration rates increased by 15.59%,22.28%,and 13.26%,respectively when compared to the control group.Additionally,the strong seedling index of seedlings treated with airflow and exogenous iron element increased by 26.02%and 31.5%,respectively.Compared to seedlings treated with exogenous iron element,the geometric mean diameter of the pith tissue cells in the stems of seedlings subjected to airflow disturbance was reduced by approximately 18.66%,while the elastic modulus and bending strength of the stems increased by 10.01%and 5.89%,respectively.Similarly,the volume of root tissue cells decreased by 19.22%,but the elastic modulus of the roots increased by 6.46%.This study confirms that airflow significantly enhances seedling resilience to abiotic stress,yielding similar or better outcomes than exogenous iron application.It provides both theoretical and practical support for using airflow disturbance as a green technology for cultivating robust seedlings.
文摘Objective:To investigate the spatial gradient of intraoperative impedance across the cochlear electrode array in pediatric cochlear implant recipients and assess its potential as a physiological indicator for the electrode-neural interface.Methods:A prospective observational study involving 56 pediatric patients underwent cochlear implantation with Cochlear Nucleus devices.Intraoperative polarized impedance and electrically evoked compound action potential(ECAP)threshold were recorded across all 1232 electrodes using AutoNRT software.Eight electrodes with open-or short-circuit were excluded,leaving 1,224 for analysis.Impedance values were categorized by cochlear region(basal,middle,apical),and electrodes with elevated impedance(10-20 kΩ)were analyzed for regional distribution and clinical relevance.Data were analyzed for spatial patterns and correlation with the ECAP threshold profiles.Results:A consistent basal-to-apical increase in impedance was observed(7.7±1.9,9.2±1.4,10.8±1.5 kΩ;p<0.001).Impedance and ECAP threshold were weakly correlated(ρ=-0.20,p<0.001;β=-1.26,p<0.001),with a positive association in the apical region(ρ=0.12,p=0.048).Electrodes with higher impedance(1020 kΩ)were less likely to show elevated or absent TNRT(OR=0.175,p=0.02).The impedance gradient persisted across age groups and was significantly correlated with ECAP threshold patterns.Conclusion:Intraoperative impedance monitoring reveals a strong and physiologically consistent gradient,with higher values in apical electrodes.This gradient reflects anatomical and tissue interface variations,which may offer a valuable physiological indicator for intraoperative electrode positioning and neural interface integrity.
基金supported by the National Natural Science Foundation of China(Nos.42077367 and 21677123).
文摘Foliar uptake of airborne metal(loid)s plays a crucial role in metal(loid)accumulation in plant organs and is influ-enced by the size and emission sources of aerosols.Given the high enrichment of toxic metal(loid)s in submicron-scale particulates(PM1),this study established a PM1 exposure system to examine airborne metal(loid)accu-mulation and foliar physiological responses in Oryza sativa L.The results showed that the concentrations of Cu,Zn,As,Pb,and Cd in the leaves and grains were influenced not only by the airborne metal(loid)levels but also by the specific nature of the PM1 particles.The quantitative model for PM1-associated Pb entry into leaf tissue indicated that foliar Pb accumulation was primarily driven by particle adhesion,followed by hydrophilic pene-tration and trans-stomatal liquid film migration,accounting for 87%–89%of the total accumulation.The strong hygroscopicity and high Pb activity of PM1 emitted from waste incineration(WI)increased the Pb absorption coefficient via the hydrophilic and liquid film migration pathway.In contrast,the high hydrophobicity of PM1 from coal burning(CB)led to greater retention of Pb on leaf surfaces.Both foliar reactive oxygen metabolism and photosynthesis indices were sensitive to air pollution.Foliar metal(loid)accumulation and airborne PM1 concentration accounted for the variance in physiological responses in rice leaves.Our results also indicated that Pb was the key element in PM1 emissions from both coal burning(CB)and waste incineration(WI)responsible for significant physiological changes in rice leaves.
基金the Australian Research Council Discovery Projects funding scheme(DP190102181,DP210101465).
文摘Accurate detection of driver fatigue is essential for improving road safety.This study investigates the effectiveness of using multimodal physiological signals for fatigue detection while incorporating uncertainty quantification to enhance the reliability of predictions.Physiological signals,including Electrocardiogram(ECG),Galvanic Skin Response(GSR),and Electroencephalogram(EEG),were transformed into image representations and analyzed using pretrained deep neu-ral networks.The extracted features were classified through a feedforward neural network,and prediction reliability was assessed using uncertainty quantification techniques such as Monte Carlo Dropout(MCD),model ensembles,and combined approaches.Evaluation metrics included standard measures(sensitivity,specificity,precision,and accuracy)along with uncertainty-aware metrics such as uncertainty sensitivity and uncertainty precision.Across all evaluations,ECG-based models consistently demonstrated strong performance.The findings indicate that combining multimodal physi-ological signals,Transfer Learning(TL),and uncertainty quantification can significantly improve both the accuracy and trustworthiness of fatigue detection systems.This approach supports the development of more reliable driver assistance technologies aimed at preventing fatigue-related accidents.
基金supported by the National Natural Science Foundation of China(32101857 and U21A20218)the China Agricultural University Corresponding Support Research Joint Fund(GSAU-DKZY-2024-001)+1 种基金the Science and Technology Program in Gansu Province,China(24ZDNA008and23JRRA1407)the Fuxi Young Talents Fund of Gansu Agricultural University,China(Gaufx-03Y10).
文摘Intercropping has been widely used in arid and semi-arid regions because of its high yield,stable productivity,and efficient utilization of resources.However,in recent years,the high yield of traditional intercropping is mainly attributed to the large amount of purchased resources such as water and fertilizer,plastic film,and mechanical power.These lead to a decline in cultivated land quality and exacerbate intercrops'premature root and canopy senescence.So,the application of traditional intercropping faces major challenges in crop production.This paper analyzes the manifestations,occurrence mechanisms,and agronomic regulatory pathways of crop senescence.The physiological and ecological characteristics of intercropping to delay root and canopy senescence of crops are reviewed in this paper.The main agronomic regulatory pathways of intercropping to delay root and canopy senescence of crops are based on above-and blow-ground interactions,including collocation of crop varieties,spatial arrangement,water and fertilizer management,and tillage and mulch practices.Future research fields of intercropping to delay root and canopy senescence should focus on the aspects of selecting and breeding special varieties,application of molecular biology techniques,and developing or applying models to predict and evaluate the root and canopy senescence process of intercrops.Comprehensive analysis and evaluation of different research results could provide a basis for enhancing intercropping delay root and canopy senescence through adopting innovative technologies for regulating the physio-ecological characteristics of intercrops.This would support developing and adopting high-yield,efficient,and sustainable intercropping systems in arid and semi-arid areas with high population density,limited land,and abundant light and heat resources.
基金funded by the National Natural Science Foundation of China(Grant No.32201896)the Zhejiang Province Key Research and Development Plan Project,China(Grant No.2022C02034)the National Modern Agricultural Industrial Technology System Construction Project,China(Grant No.CARS-01-21).
文摘Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negates the advantages of large panicle and constrains yield potential.Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration,with the sensitive period for this process commencing approximately 15‒20 d before panicle heading.Notable positional variations occur within the panicle,with significantly higher spikelet degeneration rates at the basal than at the upper positions.An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress.Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport,which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation.Sucrose serves as the main carbohydrate source for spikelet development,demonstrating an apical dominance pattern that favors spikelet formation.However,under abiotic stress,the inhibition of sucrose decomposition,rather than sucrose transport impairment,predominantly contributes to aggravated spikelet degeneration at the basal panicle positions.Brassinolide and auxin have a significant relationship with spikelet formation,potentially mediating apical dominance.Specifically,brassinolide enhances sucrose accumulation and utilization,thereby alleviating spikelet degeneration.At present,the mechanisms underlying rice spikelet degeneration have not been fully revealed,and the joint effects of hormones,carbohydrates,and carbon and nitrogen metabolism on this process require further investigation.To reduce the spikelet degeneration,the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’resilience to abiotic stress.An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.
基金supported by the National Natural Science Foundation of China(52127801)Postdoctoral Fellowship Program of CPSF under Grant Number GZC20231545,China Postdoctoral Science Foundation(2024T170557 and 2023M742224)+1 种基金Shanghai Post-doctoral Excellence Program(No.2023440)City University of Hong Kong Donation Grants(DON-RMG No.9229021 and 9220061).
文摘Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In this work,we investigated the effect of corrosion product film layers on the degradation behavior of pure Mg in physiological environments.Pure Mg shows a faster corrosion rate in simulated body fluid(SBF)compared to NaCl solution.Hydrogen evolution experiments indicate that the degradation rate of pure Mg in SBF decreases rapidly within the first 12 h but stabilizes afterward.The rapid deposition of low-solubility calcium phosphate on the pure Mg in SBF provides protection to the substrate,resulting in a gradual decrease in the degradation rates.Consequently,the corrosion product film of pure Mg formed in SBF exhibits a layered structure,with the upper layer consisting of dense Ca_(3)(PO_(4))_(2)/Mg_(3)(PO_(4))_(2) and the lower layer consisting of Mg(OH)_(2)/MgO.Electrochemical impedance spectroscopy(EIS)shows that the resistance of the corrosion product film increases over time,indicating gradual strengthening of the corrosion resistance.The 4-week degradation results in the femoral marrow cavity of mice are consistent with the result in SBF in vitro.
基金financially supported by Domaine Louata of Providence Verte Company,Agricultural Training and Research Center.
文摘Drought is one of the most severe environmental stresses affecting soybean growth and development,especially in arid and semi-arid areas.The aim of this experiment is to evaluate the effect of regulated deficit irrigation during the vegetative stages on soybean plants and determine the amount irrigation water can be reduced without affecting the physiological parameters,the crop phenology,and the yield of the soybean crop.The field experiments were conducted during two irrigation crop seasons(2021 and 2022)in Louata,Morocco.The results showed that regulated deficit irrigation regimes during the vegetative stages was combined with high temperatures and low air humidities during the beginning of flowering and the pod filling stage during 2021 in comparison with 2022,especially for 25%CWR(crop water requirements).Regulated deficit irrigation regimes reduced the stomatal conductance by 46%and 52%respectively during the first and second growing seasons by limiting CO_(2) intake for the Calvin cycle.The stomata closure increased the leaf temperature and affected the functioning of the photosynthetic apparatus by damaging the chlorophyll pigments and impairment of electron transport chains in chloroplasts.The transition from regulated deficit irrigation to 100%CWR at the beginning of flowering(R1)compensated for the photosynthetic loss,improved the growth and development of soybean plants and enhanced the yield and its components for 50%and 75%CWR.The adaptative mechanism such as the remobilization of the carbon reserved in the stems and leaves(vegetative tissues)to the grains improved the grain yield by 36.7%during 2021 and by 32.2%during 2022 and.This consequently improved the water use efficiency,the water productivity of soybean for 50%and 75%CWR and contributed to water saving with an average of 60 mm per growing season.
基金supported by the ENN Group,the ENN Energy Research Institute and National Natural Science Foundation of China(No.12205122).
文摘EHL-2 is a compact,high-field spherical tokamak designed to explore the potential of an advanced p-11B nuclear fusion reactor.Due to its high plasma current and thermal energy,it is crucial to mitigate the impact associated with disruptions to ensure the safe operation of EHL-2.This paper evaluates the performance requirements of the disruption prediction system on EHL-2,with a particular focus on applying generalizable knowledge transfer from existing devices to future ones.Furthermore,the key characteristics of disruption mitigation strategies are analyzed,and their overall mitigation performance on EHL-2 is assessed.This insight provides valuable guidance for optimizing the engineering design of EHL-2 and identifying its optimal operational regime.
文摘Hypnosis is a promising tool in the management of various conditions,such as anxiety and chronic pain.Preliminary studies have shown that hypnosis can directly affect the cardiovascular system,as it increases parasympathetic activation and reduces sympathetic activity.However,the literature related to the effects of hypnosis on cardiovascular health is scarce,mainly due to misconceptions about hypnosis among researchers and medical professionals.This opinion paper examines the role that hypnosis may play in cardiovascular health,highlighting the physiological mechanisms behind it.The evidence suggests that hypnosis has both direct(e.g.,changes in the activity of the autonomic nervous system)and indirect(e.g.,changes in healthy behaviours)effects on the cardiovascular system;however,further studies are needed to properly define its mechanisms of action and its applicability in improving cardiovascular health.Thus,this opinion paper advocates the adoption of the term“hypno-cardiac physiology”to identify a new research area that gathers experts from neuroscience and cardiovascular science with the joint aim of seeking further understanding of the effects of hypnosis on the cardiovascular system.The adoption of a dedicated term to identify the study of the cardiovascular response to hypnosis will encourage its implementation in cardiovascular health interventions,promoting awareness of its effects among the public and the healthcare community,and promoting the formation of dedicated multidisciplinary research groups and dedicated educational training for healthcare professional interested in its applications.
基金supported by the National Natural Science Foundation of China(NSFC,31971413)to D.L.
文摘Numerous bird species have adapted to rapid urbanization,navigating challenges introduced by novel stressors.While prior research has explored how urban exploiters adjust their physiology relative to their rural counterparts during breeding season,the modulation of multiple physiological markers outside of reproduction,and the maintenance of sex-specific differences,remain less understood.Using an urban exploiter,the Eurasian tree sparrow(ETS;Passer montanus),we investigated how both sexes of urban and rural populations modulate twenty-two physiological markers of nutrition,body condition,oxidative stress,and antioxidative responses during pre-breeding stage.Our results showed that urban ETSs displayed distinct glucolipid metabolism compared to rural ETSs,characterized by higher levels of free fatty acids,β-hydroxybutyric acid,high-density lipoprotein(HDL),and total cholesterol,alongside lower levels of triglycerides,glucose(Glu),and fat content(FC).No significant differences were observed in other physiological markers.Additionally,both sexes of urban ETSs exhibited similar profiles across these markers,unlike rural populations where females showed higher Glu and total protein levels and lower HDL and FC levels than males.Our findings suggest that birds adjust their nutritional physiological markers in response to urbanization,likely due to dietary differences between urban and rural environments.Furthermore,sex differences in physiological markers of nutrition,body condition,oxidative stress,and antioxidant responses appear to diminish in urban settings.
基金supported by the Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor(Guangxi Medical University),Ministry of Education(No.GKE-KF202305)the Guangxi Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor,and the Natural Science Foundation of Jiangsu Province(No.BK20211510)。
文摘Vascular disrupting agents(VDAs)can destroy tumor vasculature and lead to tumor ischemia and hypoxia,resulting in tumor necrosis.However,VDAs are easy to induce the upregulation of genes that are associated with cancer cell drug resistance and angiogenesis in tumor cells.Hypoxia-activated chemotherapy will be an ideal supplement to VDAs therapy since it can help to fully utilize the ischemia and hypoxia induced by VDAs to realize a synergistic antitumor therapeutic outcome.Here,we design a liposome whose surface is modified with a tumor-homing peptide Cys-Arg-Glu-Lys-Ala(CREKA,which can specifically target tumor vessels and stroma)and whose aqueous cavity and lipid bilayer are loaded by a hypoxia-activatable drug banoxantrone dihydrochloride(AQ4N)and a VDA combretastatin A4(CA4),respectively.CA4 can selectively target vascular endothelial cells and destroy the tumor blood vessels,which will cause the rapid inhibition of blood flow in tumor and enhance the hypoxia in the tumor region.As a consequence,AQ4N can exert its boosted cytotoxicity under the enhanced hypoxic environment.The as-prepared liposome with a uniform particle size exhibits good stability and high cancer cell killing efficacy in vitro.In addition,in vivo experiments confirm the excellent tumortargeting/accumulation,tumor vasculature-damaging,and tumor inhibition effects of the liposome.This work develops a liposomal which can achieve safe and effective tumor suppression without external stimulus excitation by only single injection,and is expected to benefit the future development of effective antitumor liposomal drugs.
基金supported by National Natural Science Foundation of China(81502688)Cooperation Research Funding of Capital Medical University(2020KJ000514)+1 种基金Cooperation Research Funding of Capital Medical University(2023KJ000814)R&D Program of Beijing Municipal Education Commission(KM202210025024).
文摘Efficient siRNA delivery is highly desirable for disease treatment.However,the application of conventional nanoparticles is limited by the inability to escape from endo-lysosomes.Herein,we report a strategy using small-molecule drugs to enhance siRNA endo-lysosomal release,addressing this challenge.We encapsulated gentamicin(GM)into the marketed Onpattro■ formulation to establish LNP-siRNA/GM nanoparticles that promote siRNA endo-lysosomal escape through endosomal disruption,mechanistically exhibiting unique functionality and synergistic effects of LNP-siRNA/GM to improve cancer therapy.Besides,GM induced reactive oxygen species(ROS)and phospholipids accumulation in endolysosomes,as well as the physical characteristics of lipid nanoparticles(LNPs)were preserved.We also revealed that GM causes endo-lysosomal swelling and disrupts the endosomal membrane to enable siRNA release,as confirmed by Galectin 3 recruitment and acridine orange release.This approach achieved∼81%mRNA-EGFR silencing,which is more than LNP-siEGFR(∼56.23%)by enhancing siRNA endo-lysosomal escape efficiency.Meanwhile,LNP-siEGFR/GM exhibited significant biological activities in HepG2 cells,driven by the synergistic effects of siEGFR and GM with the VEGF and CXCL12 downregulation of,and ROS and phospholipids upregulation.Furthermore,tumor growth was notably suppressed after intravenous injection of LNP-siEGFR/GM in tumor-bearing nude mice.The combination of EGFR-siRNA and GM could also greatly inhibit angiogenesis,be antiproliferative,and induce tumor cells apoptosis.Therefore,this GM and siRNA co-delivery system would provide an efficient strategy for siRNA endosomal escape,significantly improving knockdown in various LNPs based siRNA delivery systems and efficiently enhancing cancer therapy.
基金supported by the National Natural Science Foundation of China(No.U2333218).
文摘Airport disruptions often pose challenges in assigning aircraft to gates,resulting in infeasible planned schedules.In particular,a large number of transfer passengers miss their connections in the context of disruptions,which cause huge economic losses to airlines and serious passengers’dissatisfaction.This paper proposes a set-partitioning-based model to optimize Aircraft-Gate Reassignment with Transfer Passenger Connections(AGRP-TPC),which incorporates flexible gate-swap and aircraft-delay operations to mitigate the overall impact of disruptions.To efficiently solve the model,we introduce the concepts of additive-transfer and nonstop-transfer to handle passenger connections,and develop a Hierarchical Column-and-Row Generation(HCRG)approach guided by airport terminal space attribute.The column generation and row generation procedures solve iteratively until no new variables and constraints are generated.In addition,a follow-on strategy and a diving heuristic are designed to efficiently obtain high-quality solutions.We evaluate the proposed approach using various instances from a major Chinese international airport.Computational results demonstrate that our approach outperforms the comparison algorithms and produces good solutions within the time limit.Detailed results indicate that our approach effectively reduces overall losses in aircraft-gate reassignment following disruptions,and it can serve as an auxiliary decision-making tool for airport operators and airlines.
基金The Basic Scientific Research Project of Colleges and Universities in 2022:HRV and Brain Electrophysiological Mechanism of Electromyography Biofeedback in Improving Depression and Anxiety of College Students(Project No.:LJKMZ20221274)。
文摘Biofeedback is a behavioral intervention technology based on psychophysiology,which uses various electronic devices to collect the physiological activities of individuals in real time and provides them to themselves through various ways,so that people can actively change their own physiological changes when they perceive the changes in some physiological functions of their own,and consciously control the physiological functions of various aspects of the autonomic nervous system within a fixed range.It can play a role in improving physical health status,preventing and treating some somatic diseases.Studies have shown that biofeedback relaxation training can make the training subjects learn to regulate their own physiological functions and psychological activities,improve negative emotional states such as depression and anxiety by regulating the body function,and can also be used to assist in the treatment of other clinical diseases.As a new psychological intervention,biofeedback relaxation training has great development potential and can be better applied to the prevention and treatment of physical and mental diseases in the general population in the future.
文摘Morpho-physiological evaluation of a crop’s genetic resources is necessary to find possible genotypes to include in breeding initiatives.The objective of this study was to identify heat-tolerant wheat genotypes among 36 mutant lines using morpho-physiological indices.Seedlings of mutant lines and check varieties were grown under both normal(control)and heat-stress conditions in growth chambers.Data were recorded on root-shoot parameters(length,fresh weight,dry weight,and ratio),relative water content(RWC),stability of cell membrane,pigment content,and chlorophyll fluorescence.Two-way analysis of variance showed significant(p<0.01,p<0.001)variation among 15 morpho-physiological features in both growing conditions.Correlation studies showed that pigment-specific indices(total chl,chl a,chl b,carotenoid,Fv/Fm—chlorophyll fluorescence)had significant positive correlations with rootshoot fresh weight(RFW,SFW)and root-shoot dry weight(RDW,SDW),and negative association with relative water content,stability of cell membrane at both conditions.Hierarchical cluster analysis revealed three primary clusters where cluster III(18 genotypes)showed minimal changes in studied characters than cluster I(11 genotypes)and cluster II(13 genotypes).Results obtained from this study revealed that the identified heat-sensitive(30-2 SSH)and heat-tolerant genotypes(27-2(4),29-2(2),and 30-2 SSH)of wheat might provide useful breeding materials for enhancing wheat yield even more in this period of uncertain climate change.
基金Supported by National Natural Science Foundation of China(Grant Nos.52375279,52175001)Beijing Natural Science Foundation(Grant No.3252002).
文摘The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness in the rehabilitation therapy of forearm muscles,due to the lack of the physiological characteristics of muscle activation and sequences.An armband with eight channels was used to collect surface electromyographic signals(sEMGs)of a specific section of the forearm under the different wrist movements,palm postures,and external loads,based on the image of magnetic resonance imaging(MRI).The collected cross-sectional muscles covered almost all surface muscles.The muscle activation could be expressed clearly by enveloping the sEMG signals of 8 muscles within a single cycle.The root mean square(RMS)and the average peak value V_(P) were used to evaluate the activation intensities of dominant muscles.The activation sequences and the absolute times of dominant muscles were obtained from the envelopes of their raw sEMGs,and not influenced by the palm postures and external loads.In addition,their RMS and V_(P) under each wrist movement increased approximate linearly with external loads.The corresponding contribution ratios were first calculated to evaluate the role played by each muscle.The well-defined data of forearm muscles could provide standard references for the rehabilitation therapy of forearm muscles.
文摘Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A randomized control trial parallel group design was conducted among 45 comatose patients divided into two intervention groups,i.e.nurse voice stimulus group,receiving nurses voice with standard care,family members voice stimulus group receiving their beloved family member voice with standard care and one control group receiving only standard care in medicine intensive care unit.The intervention was provided three times a day,each lasting 5 min for 7 days in addition to standard care.Repeated measure analysis of variance and independent t-test were used to compare within and between groups,respectively.Results:The study found significant differences in Glasgow coma scale(GCS)scores within both the nurse(F=2.78,P=0.042)and family member(F=10.27,P=0.0001)voice stimulus groups over 7 days.Comparing GCS scores between intervention groups showed significant variations before(P=0.028),during(P=0.047),and after(P=0.036)the intervention on day 7.Comparing GCS scores between the family members’voice stimulus group and the control group,significant changes were observed on days 5 and 7(P=0.043,0.030,0.030,and 0.014,0.012,0.012)before,during,and after the intervention.Conclusions:The use of beloved family members’voices proved more effective in elevating the patients’level of consciousness compared to both the nurse voice stimulus group and the control group.
基金funded by the‘National Key R&D Program Subject of China’(No.2021YFD1100301)the post subsidy project of National Key R&D Program,and the Guizhou Modern Agriculture Research System(GZMARS)-Plateau characteristic vegetable industry.
文摘Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives were sampled at the vegetative growth,floral bud differentiation,and bud emergence stages.No bolting was observed in bolting-resistant‘WA’on the 130th day after planting,whereas the bolting reached 39.22%in bolting-prone‘BA’,which was significantly higher than that of‘WA’.The contents of gibberellins,abscisic acid,and zeatin riboside after floral bud differentiation in‘WA’were significantly less than in‘BA’,whereas the indoleacetic acid content in‘WA’was significantly higher than that in‘BA’before and after floral bud differentiation.The soluble sugar content and nitrate reductase activity in‘BA’were significantly higher than those in‘WA’before and during floral bud differentiation periods.However,they were significantly lower in‘BA’compared with in‘WA’after bolting due to the nutrient consumption required by reproductive growth.A transcriptome analysis determined that the differentially expressed genes related to bolting tolerance were enriched in the terms‘photoperiodism,flowering’,‘auxin-activated signaling pathway’,‘gibberellic acid mediated signaling pathway’,and‘carbohydrate metabolic process’,and this was generally consistent with the physiological data.Additionally,12 key differentially expressed genes(including isoform_203018,isoform_481005,isoform_716975,and isoform_564877)related to bolting tolerance were investigated.This research provides new information for breeding bolting-tolerant chives.
