Aims:Reed(Phragmites australis)is a plant species with a seasonal reproductive cycle;it has a very high biomass in U Minh Thuong National Park,in Vietnam.This study aims to evaluate fresh and dry biomass of the reed a...Aims:Reed(Phragmites australis)is a plant species with a seasonal reproductive cycle;it has a very high biomass in U Minh Thuong National Park,in Vietnam.This study aims to evaluate fresh and dry biomass of the reed and the production of biochar from the plants.The biochar is then used as a bio-organic fertilizer for watermelon cultivation in agriculture.Methods:To achieve these objectives the following experiments were conducted(1)investtigating the fresh and dry biomass of reeds producing biochar using local methods;(2)adsorption with pig urine and chemical fertilizers(nitrogen,phosphorus and potash)to examine the uptake of chemical components into the water environment;(3)mixing biochar with inorganic mineral fertilizers and peat to creat inorganic fertilizer–biochar formulas,followed by an analysis of the chemical compositions of the mixtures;(4)using various biochar-based fertilizers to grow watermelon with local varieties.Results:The results show that reeds produce very high for biomass biochar fertilizer production.Reed biochar can adsorb components of pig urine,such as ammonium,nitrate,nitrogen and phosphorus along with inorganic substances such as nitrogen,phosphorus and potash.Therefore this study proposes the use of this biochar for watermelon cultivation and environment treatment in polluted regions.Conclusions:Biomass and biochar of reed are very high.The biochar can adsorb nitrogen,phosphorus and potash fertilizers.Additionally,biochar can be mixed with peat and inorganic mineral fertilizers for to watermelon cultivation in Mekong Delta.Implications of the research:Forest fires in U Minh Thuong National Park,caused by reed vegetation,occur annually and result in damage to property and human livelihoods.This research not only exploits renewable raw materials but also helps control the risk of forest fires.Originality/Valeu:This study aims to provide methods for controlling forest fires by producing biochar of from reed(Phragmites australis)U Minh Thuong National Park Vietnam.This species thrives and produces a large biomass during the rainy season,supllying dry material that contributes to the intensity of forest fires in the dry season in Vietnam.展开更多
Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis ind...Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis induction,and immune-modulatory activities of extracts from three herbal medicines with historical use in traditional medicine—Acanthopanax sessiliflorus,Phragmites communis,and Pinus densiflora,as well as their combined extract(GMAS 01/COM),on human lung cancer cells(A549)and normal cell lines,including murine macrophages(RAW 264.7)and human keratinocytes(HaCaT).Methods:Plant extracts were prepared using aqueous extraction,sonication,and rotary evaporation.The total phenolic and flavonoid contents were quantified using the Folin-Ciocalteu and AlCl3 colorimetric methods,respectively.Antioxidant potential was evaluated via 2,2-Diphenyl-1-picrylhydrazyl(DPPH)scavenging and reducing power assays.Cytotoxicity was assessed using an MTT assay,while reactive oxygen species(ROS)generation was quantified using a 2′,7′-Dichlorodihydrofluorescein diacetate(DCFH-DA)assay.Anticancer properties,including colony formation inhibition and migration suppression,were examined using colony formation and wound healing assays.The expression of apoptotic and inflammatory mediators was analysed through qPCR.Results:GMAS 01 selectively induced apoptosis in A549 cells without cytotoxic effects on RAW264.7 and HaCaT cells.Mechanistically,it elevated intracellular ROS and activated the intrinsic mitochondrial apoptotic pathway,evidenced by p53 upregulation,increased Bax,and decreased Bcl-2 expression.GMAS 01 also significantly inhibited colony formation and migration in A549 cells.In RAW264.7 cells,it reduced nitric oxide(NO)production and downregulated iNOS,COX-2,IL-6,and IL-8,indicating strong immunomodulatory activity.Conclusion:GMAS 01 exhibits potent antioxidant,anti-inflammatory,and anticancer effects,likely mediated through ROS-induced mitochondrial apoptosis.However,mechanistic interpretations are limited by the absence of protein-level validation and pathway inhibition studies.Upcoming studies should aim to verify the underlying mechanisms and evaluate their potential for real-world application.展开更多
A new renewable material by use reed biochar(Phragmites autralis);a species strong grows and very high biomass,it can be exploit a renewable resources for agriculture and environmental treatment.People often used rice...A new renewable material by use reed biochar(Phragmites autralis);a species strong grows and very high biomass,it can be exploit a renewable resources for agriculture and environmental treatment.People often used rice husks as materials for organic fertilizers in agriculture.This study is a new discovere to made of reed biochar to filter N,P,K of chemical mineral fertilizer and pig urine use to provide bio-organic fertilizer for rice plant(ST 25).Methods:(1)Made of biochar by use the local method;(2)analyse the chemical indicators of locations on trunks,leaves,flowers;(3)identify chemical indicators of peatland on locations of experiment;(4)adsorpting chemical fertilizer(nitrogen,phosphorus and potash),pig urine filter by reed biochar,analize its chemical indicators;(5)use fomulas of reed plant biochar mixed peat and mineral inorganic fertilizer.Results:Made of reed biochar and it can adsorpted as ammonium,nitrate,nitrogen,phosphorus and kalium the pig urine and it also adsorpted inorganic fertilizer as nitrogen,phosphorus and Kalium.Thus it used for environmental treatment in the region polluted and planting local rice ST 25 in the experiment area.Conclusion:Biochar of reed can use to environment treatment and agriculture fertilizer;chemical indicators of bichar indentified;the mixed biochar,peat and inorganic in formulas in 5 days and 10 days were not differences;biochar can adsorb some chemical components of pig urine and nitrogen,phosphorus and kalium;biochar can mixed peat and inorganic mineral to planting rice ST 25 in Mekong Delta.展开更多
The ion levels in the epidermal bulliform cells of epidermis, mesophyll cells and cells of the vascular bundle sheath of four ecotypes of reed (Phragmites australis (Cav.) Trin. ex Steud.) were determined by means of ...The ion levels in the epidermal bulliform cells of epidermis, mesophyll cells and cells of the vascular bundle sheath of four ecotypes of reed (Phragmites australis (Cav.) Trin. ex Steud.) were determined by means of X_ray microanalysis. The results indicated that higher K +, Na +, Ca 2+ , Mg 2+ and Cl - were distributed in the vascular bundle sheath cells than in mesophyll cells and epidermal bulliform cells of the swamp ecotype. Higher Ca 2+ was found in the bulliform cells than in mesophyll cells and vascular bundle sheath cells, higher Mg 2+ in the mesophyll cells and higher K +, Na + and Cl - in the vascular bundle sheath cells of the dune ecotype. Higher Na + and Mg 2+ was determined in the mesophyll cells than in the bulliform cells and vascular bundle sheath cells, and higher K +, Ca 2+ and Cl - in the vascular bundle sheath cells of the light salt meadow ecotype. In the heavy salt meadow ecotype, higher Na + and Mg 2+ were accumulated in the bulliform cells than in mesophyll cells and vascular bundle sheath cells, but higher K +, Ca 2+ and Cl - in the mesophyll cells. Furthermore, the distributional conditions of the above five ions in leaf cells of the four ecotypes and their significance in the physiological adaptation of reed to habitat were discussed in detail.展开更多
R5002_12, a salt tolerant line of Phragmites communis Trin., which was obtained from ethyl methane sulfonate (EMS) treated callus selected under saline stress, was compared with its wild line in respect to their m...R5002_12, a salt tolerant line of Phragmites communis Trin., which was obtained from ethyl methane sulfonate (EMS) treated callus selected under saline stress, was compared with its wild line in respect to their molecular biological, physiological and biochemical characterizations. Five arbitrary primers were screened which showed differences in DNA amplified polymorphism between the variant and its wild line. Some new proteins appeared in the salt tolerant plant under salt stress. Electrophoresis of peroxidase and esterase also showed some differences in isozyme expression between them. The chlorophyll content of the variant was higher than that of the original variety, whether the plants were under salt stress or not.展开更多
Shallow lake eutrophication is a global environmental issue. This study investigated the effects of water level variation and nutrient loadings on the growth and nutrient accumulation of Phragmites australis （reed） ...Shallow lake eutrophication is a global environmental issue. This study investigated the effects of water level variation and nutrient loadings on the growth and nutrient accumulation of Phragmites australis （reed） by field samplings in Baiyangdian Lake, the largest shallow lake of northern China. The field samplings were conducted in two sites of different nutrient loadings during the whole growth period of reeds, and three types of zones with different water depths were chosen for each site, including the terrestrial zone with water level below the ground, the ecotone zone with the water level varying from belowground to aboveground, and the submerged zone with water level above the ground. The result showed that reed growth was more limited by water level variation than nutrient loadings. The average stem lengths and diameters in terrestrial zones were about 26.3%-27.5% and 7.2%-12.0% higher than those in submerged zones, respectively. Similarly, the terrestrial status increased the aboveground biomass of reeds by 36.6%-51.8% compared with the submerged status. Both the nutrient concentrations and storages in the aboveground reeds were mainly influenced by the nutrient loadings in surface water and sediment rather than the water level variation of the reed growth environment, and the nutrient storages reached their maxima in late August or early September. It was observed that the maximum nitrogen storage occurred in the terrestrial zone with higher nutrient loadings, with the value of 74.5 g/m2. This study suggested that water level variation and nutrient loadings should be considered when using reeds to control and remediate eutrophication of shallow lakes.展开更多
Two plant populations of Phragmites australis and Typha orientalis grown in gravel and sediment substrate were studied to assess their capabilities for purifying polluted water in Taihu Lake, China. The substrate disp...Two plant populations of Phragmites australis and Typha orientalis grown in gravel and sediment substrate were studied to assess their capabilities for purifying polluted water in Taihu Lake, China. The substrate displayed most significant effects on the suspended matter （P 〈 0.01）, with the reduction of 76%-87% and 52%--63% for P. australis, and 83%-86% and 45%-62% for T. orientalis in gravel substrate and sediment substrate, respectively. Both species and substrates significantly decreased the N and P concentrations of water body （P 〈 0.01）. P. australis showed higher total N and P concentrations in tissues than T. orientalis and had a greater potential to remove nutrients from the lake. Phosphate was easily to concentrate in the belowground tissues, while nitrate concentration was higher in leaf and stalk. Therefore, harvesting the aboveground tissues could take most of nitrate out of the sediment. The saturate photosynthetic rate （Asat） of P. australis was higher than that of T. orientalis when grown in sediment substrate. But instance water-use- efficiency （WUEi） （A/E） and intrinsic water use efficiency （A/gs） showed the maximum values of two species grown in river water. With significant difference in gs, however, intercellular CO2 concentration （Ci） had no obvious difference in two species which indicated that high Asat value of P. australis might result from the increased carboxylation capacity of the mesophyll, because of the central role of N in photosynthetic enzymes. Our findings suggest that the plants could absorb most of nitrogen in polluted water, while gravel displayed a high capacity for absorbing the suspended matters and phosphate salts. Therefore, biological and physiological pathways for pollutant removal should be integrated.展开更多
The field trial was established to investigate the effects of planting condition(soil water content,soil buried depth,rhizome length)and time on the survival rate and growth of Phragmites australis rhizomes.The result...The field trial was established to investigate the effects of planting condition(soil water content,soil buried depth,rhizome length)and time on the survival rate and growth of Phragmites australis rhizomes.The results indicated that survival rate and growth of Phragmites australis were affected by soil water content and rhizome length significantly,but not by soil buried depth.The survival rate of Phragmites australis in moist condition was higher than those in natural and flooding conditions.Rhizomes length did not affect survival rate,height and shoot number,but influenced biomass and buds number,which were higher with 15 cm length than those with 30 cm length.Based on the suitable soil water content(moisture)and rhizome length(15 cm),the survival rate of Phragmites australis was the highest when they were planted in May(91%)comparing to June and July.The number of shoots and buds were the highest in June,which had more suitable temperature and light for Phragmites australis to grow.Therefore,the optimizing transplanting condition of Phragmites australis was that rhizomes of 15 cm with some buds were planted in May with moisture(soil water content).展开更多
As widespread wetland plants,Phragmites play a vital role in water purification and are widely utilized in constructed wetlands(accounting for 15.5%of applied wetland plants)as a natural alternative to wastewater trea...As widespread wetland plants,Phragmites play a vital role in water purification and are widely utilized in constructed wetlands(accounting for 15.5%of applied wetland plants)as a natural alternative to wastewater treatment.However,despite such common applications,current understanding of the basic composition of the Phragmites root-inhabiting microbiome and the complex functions of each member of this microbiome remains incomplete,especially regarding pollution remediation.This review summa-rizes the advances that have been made in ecological and biochemical research on the Phragmites root microbiome,including bacteria,archaea,and fungi.Based on next-generation sequencing,microbial com-munity compositions have been profiled under various environmental conditions.Furthermore,culture-based methods have helped to clarify the functions of the microbiome,such as metal iron stabilization,organic matter degradation,and nutrient element transformation.The unique community structure and functions are highly impacted by Phragmites lineages and environmental factors such as salinity.Based on the current understanding of the Phragmites root microbiome,we propose that synthetic microbial com-munities and iron–manganese plaque could be applied and intensified in constructed wetlands to help promote their water purification performance.展开更多
Emissions of biogenic sulfur gases(hydrogen sulfide(H_2S) and carbonyl sulfide(COS)) from Phragmites australis coastal marsh in the Yellow River estuary of China were determined during April to December in 2014 using ...Emissions of biogenic sulfur gases(hydrogen sulfide(H_2S) and carbonyl sulfide(COS)) from Phragmites australis coastal marsh in the Yellow River estuary of China were determined during April to December in 2014 using static chamber-gas chromatography technique with monthly sampling. The results showed that the fluxes of H_2S and COS both had distinct seasonal and diurnal variations. The H_2S fluxes ranged from 0.09 μg/(m^2·h) to 7.65 μg/(m^2·h), and the COS fluxes ranged from –1.10 μg/(m^2·h) to 3.32 μg/(m^2·h). The mean fluxes of H_2S and COS from the P. australis coastal marsh were 2.28 μg/(m^2·h), and 1.05 μg/(m^2·h), respectively. The P. australis coastal marsh was the emission source of both H_2S and COS over the whole year. Fluxes of H_2S and COS were both higher in plant growing season than in the non-growing season. Temperature had a dramatic effect on the H_2S emission flux, while the correlations between COS flux and the environmental factors were not found during sampling periods. More in-depth and comprehensive research on other related factors, such as vegetation, sediment substrates, and tidal action is needed to discover and further understand the key factors and the release mechanism of sulfur gases.展开更多
Little information is available on biogenic elements(carbon, nitrogen, phosphorus and sulfur) and the ecological stoichiometric characteristics of plants in coastal wetlands. To investigate the contents of carbon, nit...Little information is available on biogenic elements(carbon, nitrogen, phosphorus and sulfur) and the ecological stoichiometric characteristics of plants in coastal wetlands. To investigate the contents of carbon, nitrogen, phosphorus and sulfur of plants, and their ecological stoichiometric characteristics in the Yellow(Huanghe) River Delta, plant samples were collected from two typical salt marshes(Suaeda salsa and Phragmites australis wetlands) during the period of from August to October in 2007, and the ratios of C/N, C/P, N/P, C/N/P and C/N/P/S were calculated. Results showed that during the studying period, plant C, N and P were lower than the global average values, and plant N and P were lower than the China's average values. Leaf C and S in Suaeda salsa were significantly lower than those in Phragmites australis(P < 0.05), and leaf N and P in Suaeda salsa and Phragmites australis showed no significant differences(P > 0.05). Average C/N ratios were 23.75 in leaf, 73.36 in stem, 65.67 in root of Suaeda salsa, and 33.77 in leaf, 121.68 in stem, 97.13 in root of Phragmites australis. Average C/N ratios of Suaeda salsa and Phragmites australis were all great than 25, indicating the salt marsh in the Yellow River Delta is an N limitation system. Average C/P ratios were 276.78 in leaf, 709.28 in stem and 1031.32 in root of Suaeda salsa, and 536.94 in leaf, 768.13 in stem and 875.22 in root of Phragmites australis. The average N/P ratios of Suaeda salsa were 12.92 in leaf, 10.77 in stem and 10.91 in root, and the average N/P ratios of Phragmites australis were 16.40 in leaf, 7.40 in stem and 6.92 in root, indicating the Suaeda salsa wetlands were N limited and Phragmites australis wetlands were N limited in August and P limited in October in 2007. The average C/N, C/P and C/N/P ratios in Suaeda salsa and Pragmites australis were higher than the global average values, indicating the lower quality of organic matter provided by wetland plants in the Yellow River delta.展开更多
Abstract:Hydrological regimes influence ecological patterns and processes as well as alter rates of wetland evapotranspiration.This study aimed to investigate the impact of groundwater fluctuation on evapotranspiratio...Abstract:Hydrological regimes influence ecological patterns and processes as well as alter rates of wetland evapotranspiration.This study aimed to investigate the impact of groundwater fluctuation on evapotranspiration of Phragmites australis.Supported by field data obtained from the Baiyangdian Lake in northern China,the variations in groundwater levels were explored,and the changes in soil water and evapotranspiration of reed were analyzed to investigate different groundwater level scenarios using HYDRUS-1D model.The results showed that soil water content,recharged by groundwater,remained stable in the lower soil layer but varied strongly in the upper layer of the soil profile;in comparison to evaporation,Phragmites australis transpiration contributed significantly more to the overall evapotranspiration rate;the high levels of evapotranspiration could be maintained when groundwater levels vary between 1.0 m and 1.8 m,while it was reduced with an increase in groundwater levels as a result of water stress conditions.The results also indicated that the evapotranspiration of Phragmites australis could maintain higher evapotranspiration rates under natural water levels.The evapotranspiration,in other words,might be the main water consumer,but it nevertheless has little effect on water levels during water shortages.The evapotranspiration of Phragmites australis responded to the changes in groundwater levels could help researchers understand water requirements of the wetlands and establish suitable water levels for the wetlands.展开更多
Liberty State Park in New Jersey,USA,is a ＂brownfield＂ site containing various levels of contaminants.To investigate metal uptake and distributions in plants on the brownfield site,Phragmites australis and Typha lat...Liberty State Park in New Jersey,USA,is a ＂brownfield＂ site containing various levels of contaminants.To investigate metal uptake and distributions in plants on the brownfield site,Phragmites australis and Typha latifolia were collected in Liberty State Park during the growing season（May–September）in 2011 at two sites with the high and low metal loads,respectively.The objective of this study was to understand the metal（Fe,Mn,Cu,Pb and Zn）concentration and spatial distributions in P.australis and T.latifolia root systems with micro-meter scale resolution using synchrotron X-ray microfluorescence（μXRF）and synchrotron X-ray computed microtomography（μCMT）techniques.The root structure measurement by synchrotron μCMT showed that high X-ray attenuation substance appeared in the epidermis.Synchrotron μXRF measurement showed that metal concentrations and distributions in the root cross-section between epidermis and vascular tissue were statistically different.Significant correlations were found between metals（Cu,Mn,Pb and Zn）and Fe in the epidermis,implying that metals were scavenged by Fe oxides.The results from this study suggest that the expression of metal transport and accumulation within the root systems may be element specific.The information derived from this study can improve our current knowledge of the wetland plant ecological function in brownfield remediation.展开更多
The aim of this study was to determine the species composition, biodiversity and, relative abundance of epiphytic algae and their relationship with environmental variables on Typha latifolia and Phragmites australis a...The aim of this study was to determine the species composition, biodiversity and, relative abundance of epiphytic algae and their relationship with environmental variables on Typha latifolia and Phragmites australis at Lake Gala(National Park). Epiphytic algae were gathered monthly by collecting aquatic plants between March 2014 and November 2014. In the epiphytic flora were a total of 133 taxa were identified, 107 taxa were identified on T. latifolia and 96 were discovered on P. australis. While the mean species richness, species diversity and evenness values of the algae identified on T. latifolia were 46, 1.85 and 0.51 respectively, these values were respectively 43, 1.51 and 0.43 on P. australis. While diatoms were generally dominant, other dominant groups in the epiphytic flora included green algae and blue-green algae. The algae that had the highest relative biovolume on T. latifolia were Spirogyra affinis, Oscillatoria sancta and Gomphonema acuminatum, while the algae that had the highest relative biovolume on P. australis were E pithemia adnata, Oscillatoria sancta and R hopalodia gibba. Results show that species composition of epiphytic algae was different, but diversity values were similar on all the macrophytes. The hydrological pulse is one of the most important factors determining the physical and chemical environment of the epiphytic algal community. It was found that some environmental factors were highly eff ective on community distribution in the epiphyton. Additionally, it was observed that some epiphytic algae species had a substrate preference between T. latifolia and P. australis.展开更多
Reeds are widely distributed in drought and high salt conditions of northwestern China. Leaf epidermal micromorphology, anatomy, chloroplast ultrastructure and physio-chemical characteristics due to long-term adaptati...Reeds are widely distributed in drought and high salt conditions of northwestern China. Leaf epidermal micromorphology, anatomy, chloroplast ultrastructure and physio-chemical characteristics due to long-term adaptation in the natural habitats of common reed （Phragmites communis Trin.） contrasted considerably among three different ecotypes： dune reed （DR）, Gobi salt reed （GSR） and swamp reed （SR）. The main objective of the present study is to determine the adapting characteristics of morphology, anatomy and physiological responses of thin roots in DR, GSR and SR. The results show that root length density was higher in SR and few root hairs were observed in DR. Cross-section anatomical features show that each ecotype has an endodermis and exodermis, while cortex thickness and proportion of root cortical aerenchyma and stele in root structure varied among the three ecotypes. The stele and xylem share a larger area in DR compared to GSR and SR. GSR has a large proportion of the cortex with radialized distribution of aerenchyma cells spacing, and the cortex has a peripheral, mechanically stiff ring in the exodermis. SEM and TEM microscope images show that GSR has a scle- renchyma ring with high lignification in the exodermis. The physio-chemical parameters show that GSR had a higher level of stress tolerance than DR. These findings indicate that developed water-absorbing tissues were largely distributed in the root structure of DR, and a main framework with supporting function spacing with aerenchyma was dominant in GSR in the long term adaptation to their natural habitats, respectively.展开更多
Passive-warming, open-top chambers(OTCs) are widely applied for studying the effects of future climate warming on coastal wetlands. In this study, a set of six OTCs were established at a Phragmites wetland located in ...Passive-warming, open-top chambers(OTCs) are widely applied for studying the effects of future climate warming on coastal wetlands. In this study, a set of six OTCs were established at a Phragmites wetland located in the Yellow River Delta of Dongying City, China. With data collected through online transmission and in-situ sensors, the attributes and patterns of realized OTCs warming are demonstrated.The authors also quantified the preliminary influence of experimental chamber warming on plant traits.OTCs produced an elevated average air temperature of 0.8°C(relative to controls) during the growing season(June to October) of 2018, and soil temperatures actually decreased by 0.54°C at a depth of 5 cm and 0.46°C at a depth of 30 cm in the OTCs. Variations in diel patterns of warming depend greatly on the heat sources of incoming radiation in the daytime versus soil heat flux at night. Warming effects were often larger during instantaneous analyses and influenced OTCs air temperatures from-2.5°C to 8.3°C dependent on various meteorological conditions at any given time, ranging from cooling influences from vertical heat exchange and vegetation to radiation-associated warming. Night-time temperature depressions in the OTCs were due to the low turbulence inside OTCs and changes in surface soilatmosphere heat transfer. Plant shoot density, basal diameter, and biomass of Phragmites decreased by23.2%, 6.3%, and 34.0%, respectively, under experimental warming versus controls, and plant height increased by 4.3%, reflecting less carbon allocation to stem structures as plants in the OTCs experienced simultaneous wind buffering. While these passive-warming OTCs created the desired warming effects both to the atmosphere and soils, pest damages on the plant leaves and lodging within the OTCs were extensive and serious, creating the need to consider control options for these chambers and the replicated OTCs studies underway in other Chinese Phragmites marshes(Panjin and Yancheng).展开更多
文摘Aims:Reed(Phragmites australis)is a plant species with a seasonal reproductive cycle;it has a very high biomass in U Minh Thuong National Park,in Vietnam.This study aims to evaluate fresh and dry biomass of the reed and the production of biochar from the plants.The biochar is then used as a bio-organic fertilizer for watermelon cultivation in agriculture.Methods:To achieve these objectives the following experiments were conducted(1)investtigating the fresh and dry biomass of reeds producing biochar using local methods;(2)adsorption with pig urine and chemical fertilizers(nitrogen,phosphorus and potash)to examine the uptake of chemical components into the water environment;(3)mixing biochar with inorganic mineral fertilizers and peat to creat inorganic fertilizer–biochar formulas,followed by an analysis of the chemical compositions of the mixtures;(4)using various biochar-based fertilizers to grow watermelon with local varieties.Results:The results show that reeds produce very high for biomass biochar fertilizer production.Reed biochar can adsorb components of pig urine,such as ammonium,nitrate,nitrogen and phosphorus along with inorganic substances such as nitrogen,phosphorus and potash.Therefore this study proposes the use of this biochar for watermelon cultivation and environment treatment in polluted regions.Conclusions:Biomass and biochar of reed are very high.The biochar can adsorb nitrogen,phosphorus and potash fertilizers.Additionally,biochar can be mixed with peat and inorganic mineral fertilizers for to watermelon cultivation in Mekong Delta.Implications of the research:Forest fires in U Minh Thuong National Park,caused by reed vegetation,occur annually and result in damage to property and human livelihoods.This research not only exploits renewable raw materials but also helps control the risk of forest fires.Originality/Valeu:This study aims to provide methods for controlling forest fires by producing biochar of from reed(Phragmites australis)U Minh Thuong National Park Vietnam.This species thrives and produces a large biomass during the rainy season,supllying dry material that contributes to the intensity of forest fires in the dry season in Vietnam.
文摘Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis induction,and immune-modulatory activities of extracts from three herbal medicines with historical use in traditional medicine—Acanthopanax sessiliflorus,Phragmites communis,and Pinus densiflora,as well as their combined extract(GMAS 01/COM),on human lung cancer cells(A549)and normal cell lines,including murine macrophages(RAW 264.7)and human keratinocytes(HaCaT).Methods:Plant extracts were prepared using aqueous extraction,sonication,and rotary evaporation.The total phenolic and flavonoid contents were quantified using the Folin-Ciocalteu and AlCl3 colorimetric methods,respectively.Antioxidant potential was evaluated via 2,2-Diphenyl-1-picrylhydrazyl(DPPH)scavenging and reducing power assays.Cytotoxicity was assessed using an MTT assay,while reactive oxygen species(ROS)generation was quantified using a 2′,7′-Dichlorodihydrofluorescein diacetate(DCFH-DA)assay.Anticancer properties,including colony formation inhibition and migration suppression,were examined using colony formation and wound healing assays.The expression of apoptotic and inflammatory mediators was analysed through qPCR.Results:GMAS 01 selectively induced apoptosis in A549 cells without cytotoxic effects on RAW264.7 and HaCaT cells.Mechanistically,it elevated intracellular ROS and activated the intrinsic mitochondrial apoptotic pathway,evidenced by p53 upregulation,increased Bax,and decreased Bcl-2 expression.GMAS 01 also significantly inhibited colony formation and migration in A549 cells.In RAW264.7 cells,it reduced nitric oxide(NO)production and downregulated iNOS,COX-2,IL-6,and IL-8,indicating strong immunomodulatory activity.Conclusion:GMAS 01 exhibits potent antioxidant,anti-inflammatory,and anticancer effects,likely mediated through ROS-induced mitochondrial apoptosis.However,mechanistic interpretations are limited by the absence of protein-level validation and pathway inhibition studies.Upcoming studies should aim to verify the underlying mechanisms and evaluate their potential for real-world application.
基金funding from of Kien Giang University of MOETV,pay the fee of this article from Authors which by themselves,research funding from Ministry of Education and Training of Vietnam(MOETV)funding Number B2024–KGU 01-2024.
文摘A new renewable material by use reed biochar(Phragmites autralis);a species strong grows and very high biomass,it can be exploit a renewable resources for agriculture and environmental treatment.People often used rice husks as materials for organic fertilizers in agriculture.This study is a new discovere to made of reed biochar to filter N,P,K of chemical mineral fertilizer and pig urine use to provide bio-organic fertilizer for rice plant(ST 25).Methods:(1)Made of biochar by use the local method;(2)analyse the chemical indicators of locations on trunks,leaves,flowers;(3)identify chemical indicators of peatland on locations of experiment;(4)adsorpting chemical fertilizer(nitrogen,phosphorus and potash),pig urine filter by reed biochar,analize its chemical indicators;(5)use fomulas of reed plant biochar mixed peat and mineral inorganic fertilizer.Results:Made of reed biochar and it can adsorpted as ammonium,nitrate,nitrogen,phosphorus and kalium the pig urine and it also adsorpted inorganic fertilizer as nitrogen,phosphorus and Kalium.Thus it used for environmental treatment in the region polluted and planting local rice ST 25 in the experiment area.Conclusion:Biochar of reed can use to environment treatment and agriculture fertilizer;chemical indicators of bichar indentified;the mixed biochar,peat and inorganic in formulas in 5 days and 10 days were not differences;biochar can adsorb some chemical components of pig urine and nitrogen,phosphorus and kalium;biochar can mixed peat and inorganic mineral to planting rice ST 25 in Mekong Delta.
文摘The ion levels in the epidermal bulliform cells of epidermis, mesophyll cells and cells of the vascular bundle sheath of four ecotypes of reed (Phragmites australis (Cav.) Trin. ex Steud.) were determined by means of X_ray microanalysis. The results indicated that higher K +, Na +, Ca 2+ , Mg 2+ and Cl - were distributed in the vascular bundle sheath cells than in mesophyll cells and epidermal bulliform cells of the swamp ecotype. Higher Ca 2+ was found in the bulliform cells than in mesophyll cells and vascular bundle sheath cells, higher Mg 2+ in the mesophyll cells and higher K +, Na + and Cl - in the vascular bundle sheath cells of the dune ecotype. Higher Na + and Mg 2+ was determined in the mesophyll cells than in the bulliform cells and vascular bundle sheath cells, and higher K +, Ca 2+ and Cl - in the vascular bundle sheath cells of the light salt meadow ecotype. In the heavy salt meadow ecotype, higher Na + and Mg 2+ were accumulated in the bulliform cells than in mesophyll cells and vascular bundle sheath cells, but higher K +, Ca 2+ and Cl - in the mesophyll cells. Furthermore, the distributional conditions of the above five ions in leaf cells of the four ecotypes and their significance in the physiological adaptation of reed to habitat were discussed in detail.
文摘R5002_12, a salt tolerant line of Phragmites communis Trin., which was obtained from ethyl methane sulfonate (EMS) treated callus selected under saline stress, was compared with its wild line in respect to their molecular biological, physiological and biochemical characterizations. Five arbitrary primers were screened which showed differences in DNA amplified polymorphism between the variant and its wild line. Some new proteins appeared in the salt tolerant plant under salt stress. Electrophoresis of peroxidase and esterase also showed some differences in isozyme expression between them. The chlorophyll content of the variant was higher than that of the original variety, whether the plants were under salt stress or not.
基金supported by the Major State Basic Research Development Program (No.2010CB951104)the Program for New Century Excellent Talents in University (No. NCET-09-0233)the National Water Pollution Control and Treatment Project in China (No.2008ZX07209-009)
文摘Shallow lake eutrophication is a global environmental issue. This study investigated the effects of water level variation and nutrient loadings on the growth and nutrient accumulation of Phragmites australis （reed） by field samplings in Baiyangdian Lake, the largest shallow lake of northern China. The field samplings were conducted in two sites of different nutrient loadings during the whole growth period of reeds, and three types of zones with different water depths were chosen for each site, including the terrestrial zone with water level below the ground, the ecotone zone with the water level varying from belowground to aboveground, and the submerged zone with water level above the ground. The result showed that reed growth was more limited by water level variation than nutrient loadings. The average stem lengths and diameters in terrestrial zones were about 26.3%-27.5% and 7.2%-12.0% higher than those in submerged zones, respectively. Similarly, the terrestrial status increased the aboveground biomass of reeds by 36.6%-51.8% compared with the submerged status. Both the nutrient concentrations and storages in the aboveground reeds were mainly influenced by the nutrient loadings in surface water and sediment rather than the water level variation of the reed growth environment, and the nutrient storages reached their maxima in late August or early September. It was observed that the maximum nitrogen storage occurred in the terrestrial zone with higher nutrient loadings, with the value of 74.5 g/m2. This study suggested that water level variation and nutrient loadings should be considered when using reeds to control and remediate eutrophication of shallow lakes.
基金supported by the Control and Treatment of Water Pollution Implementation Scheme of the National Important Science and Technology Project(No. 2008ZX07526-002)the Hi-Tech Research and Development Program (863) of China (No. 2002AA601012-06)the National Basic Research Program (973) of China (No. 2002CB412409)
文摘Two plant populations of Phragmites australis and Typha orientalis grown in gravel and sediment substrate were studied to assess their capabilities for purifying polluted water in Taihu Lake, China. The substrate displayed most significant effects on the suspended matter （P 〈 0.01）, with the reduction of 76%-87% and 52%--63% for P. australis, and 83%-86% and 45%-62% for T. orientalis in gravel substrate and sediment substrate, respectively. Both species and substrates significantly decreased the N and P concentrations of water body （P 〈 0.01）. P. australis showed higher total N and P concentrations in tissues than T. orientalis and had a greater potential to remove nutrients from the lake. Phosphate was easily to concentrate in the belowground tissues, while nitrate concentration was higher in leaf and stalk. Therefore, harvesting the aboveground tissues could take most of nitrate out of the sediment. The saturate photosynthetic rate （Asat） of P. australis was higher than that of T. orientalis when grown in sediment substrate. But instance water-use- efficiency （WUEi） （A/E） and intrinsic water use efficiency （A/gs） showed the maximum values of two species grown in river water. With significant difference in gs, however, intercellular CO2 concentration （Ci） had no obvious difference in two species which indicated that high Asat value of P. australis might result from the increased carboxylation capacity of the mesophyll, because of the central role of N in photosynthetic enzymes. Our findings suggest that the plants could absorb most of nitrogen in polluted water, while gravel displayed a high capacity for absorbing the suspended matters and phosphate salts. Therefore, biological and physiological pathways for pollutant removal should be integrated.
基金Under the auspices of the National Natural Science Foundation of China(31100403)National Basic Research Program of China(No:2012CB956100)
文摘The field trial was established to investigate the effects of planting condition(soil water content,soil buried depth,rhizome length)and time on the survival rate and growth of Phragmites australis rhizomes.The results indicated that survival rate and growth of Phragmites australis were affected by soil water content and rhizome length significantly,but not by soil buried depth.The survival rate of Phragmites australis in moist condition was higher than those in natural and flooding conditions.Rhizomes length did not affect survival rate,height and shoot number,but influenced biomass and buds number,which were higher with 15 cm length than those with 30 cm length.Based on the suitable soil water content(moisture)and rhizome length(15 cm),the survival rate of Phragmites australis was the highest when they were planted in May(91%)comparing to June and July.The number of shoots and buds were the highest in June,which had more suitable temperature and light for Phragmites australis to grow.Therefore,the optimizing transplanting condition of Phragmites australis was that rhizomes of 15 cm with some buds were planted in May with moisture(soil water content).
基金supported by the National Natural Science Foundation of China (51778603)the Chinese Academy of Sciences(QYZDY-SSW-DQC004)
文摘As widespread wetland plants,Phragmites play a vital role in water purification and are widely utilized in constructed wetlands(accounting for 15.5%of applied wetland plants)as a natural alternative to wastewater treatment.However,despite such common applications,current understanding of the basic composition of the Phragmites root-inhabiting microbiome and the complex functions of each member of this microbiome remains incomplete,especially regarding pollution remediation.This review summa-rizes the advances that have been made in ecological and biochemical research on the Phragmites root microbiome,including bacteria,archaea,and fungi.Based on next-generation sequencing,microbial com-munity compositions have been profiled under various environmental conditions.Furthermore,culture-based methods have helped to clarify the functions of the microbiome,such as metal iron stabilization,organic matter degradation,and nutrient element transformation.The unique community structure and functions are highly impacted by Phragmites lineages and environmental factors such as salinity.Based on the current understanding of the Phragmites root microbiome,we propose that synthetic microbial com-munities and iron–manganese plaque could be applied and intensified in constructed wetlands to help promote their water purification performance.
基金National Nature Science Foundation of China(No.41103036)Natural Science Foundation of Shandong Province,China(No.BS2009HZ013)
文摘Emissions of biogenic sulfur gases(hydrogen sulfide(H_2S) and carbonyl sulfide(COS)) from Phragmites australis coastal marsh in the Yellow River estuary of China were determined during April to December in 2014 using static chamber-gas chromatography technique with monthly sampling. The results showed that the fluxes of H_2S and COS both had distinct seasonal and diurnal variations. The H_2S fluxes ranged from 0.09 μg/(m^2·h) to 7.65 μg/(m^2·h), and the COS fluxes ranged from –1.10 μg/(m^2·h) to 3.32 μg/(m^2·h). The mean fluxes of H_2S and COS from the P. australis coastal marsh were 2.28 μg/(m^2·h), and 1.05 μg/(m^2·h), respectively. The P. australis coastal marsh was the emission source of both H_2S and COS over the whole year. Fluxes of H_2S and COS were both higher in plant growing season than in the non-growing season. Temperature had a dramatic effect on the H_2S emission flux, while the correlations between COS flux and the environmental factors were not found during sampling periods. More in-depth and comprehensive research on other related factors, such as vegetation, sediment substrates, and tidal action is needed to discover and further understand the key factors and the release mechanism of sulfur gases.
基金Under the auspices of National Key R&D Program of China(No.2017YFC0505906)National Natural Science Foundation of China(No.51639001,51379012)Interdiscipline Research Funds of Beijing Normal University
文摘Little information is available on biogenic elements(carbon, nitrogen, phosphorus and sulfur) and the ecological stoichiometric characteristics of plants in coastal wetlands. To investigate the contents of carbon, nitrogen, phosphorus and sulfur of plants, and their ecological stoichiometric characteristics in the Yellow(Huanghe) River Delta, plant samples were collected from two typical salt marshes(Suaeda salsa and Phragmites australis wetlands) during the period of from August to October in 2007, and the ratios of C/N, C/P, N/P, C/N/P and C/N/P/S were calculated. Results showed that during the studying period, plant C, N and P were lower than the global average values, and plant N and P were lower than the China's average values. Leaf C and S in Suaeda salsa were significantly lower than those in Phragmites australis(P < 0.05), and leaf N and P in Suaeda salsa and Phragmites australis showed no significant differences(P > 0.05). Average C/N ratios were 23.75 in leaf, 73.36 in stem, 65.67 in root of Suaeda salsa, and 33.77 in leaf, 121.68 in stem, 97.13 in root of Phragmites australis. Average C/N ratios of Suaeda salsa and Phragmites australis were all great than 25, indicating the salt marsh in the Yellow River Delta is an N limitation system. Average C/P ratios were 276.78 in leaf, 709.28 in stem and 1031.32 in root of Suaeda salsa, and 536.94 in leaf, 768.13 in stem and 875.22 in root of Phragmites australis. The average N/P ratios of Suaeda salsa were 12.92 in leaf, 10.77 in stem and 10.91 in root, and the average N/P ratios of Phragmites australis were 16.40 in leaf, 7.40 in stem and 6.92 in root, indicating the Suaeda salsa wetlands were N limited and Phragmites australis wetlands were N limited in August and P limited in October in 2007. The average C/N, C/P and C/N/P ratios in Suaeda salsa and Pragmites australis were higher than the global average values, indicating the lower quality of organic matter provided by wetland plants in the Yellow River delta.
基金Under the auspices of the Major State Basic Research Development Program of China(973 Program)(2010CB951104)Fundamental Research Funds for the Central Universities(2012LYB12)+1 种基金Beijing Higher Education Young Elite Teacher Project(YETP0259)special fund of State Key Joint Laboratory of Environment Simulation and Pollution Control(14L01ESPC)
文摘Abstract:Hydrological regimes influence ecological patterns and processes as well as alter rates of wetland evapotranspiration.This study aimed to investigate the impact of groundwater fluctuation on evapotranspiration of Phragmites australis.Supported by field data obtained from the Baiyangdian Lake in northern China,the variations in groundwater levels were explored,and the changes in soil water and evapotranspiration of reed were analyzed to investigate different groundwater level scenarios using HYDRUS-1D model.The results showed that soil water content,recharged by groundwater,remained stable in the lower soil layer but varied strongly in the upper layer of the soil profile;in comparison to evaporation,Phragmites australis transpiration contributed significantly more to the overall evapotranspiration rate;the high levels of evapotranspiration could be maintained when groundwater levels vary between 1.0 m and 1.8 m,while it was reduced with an increase in groundwater levels as a result of water stress conditions.The results also indicated that the evapotranspiration of Phragmites australis could maintain higher evapotranspiration rates under natural water levels.The evapotranspiration,in other words,might be the main water consumer,but it nevertheless has little effect on water levels during water shortages.The evapotranspiration of Phragmites australis responded to the changes in groundwater levels could help researchers understand water requirements of the wetlands and establish suitable water levels for the wetlands.
基金supported in part by the Margaret and Herman Sokol Foundation(HF)China Scholarship Council(YQ)+6 种基金the State Key Laboratory of Estuarine and Coastal Research Open Research Fund(Ref #:SKLEC-KF201304)(HF,WZ,LY,YQ)supported in part by the U.S.Department of Energy,Office of Science,Office of Workforce Development for Teachers and Scientists(WDTS)under the Visiting Faculty Program(VFP)(HF)Portions of this work were performed at Beamline X27A,National Synchrotron Light Source(NSLS),and Biosciences Department,Brookhaven National LaboratoryWork in Bioscience Department,BNL,was partially supported by the Division of Chemical Sciences,Geosciences,and Biosciences,Office of Basic Energy Sciences of the US Department of Energy through Grant DEAC0298CH10886the National Science Foundation through grant MCB-1051675(CJL)X27A is supported in part by the U.S.Department of Energy--Geosciences(DE-FG02-92ER14244 to The University of Chicago-CARS)Use of the NSLS was supported by the U.S.Department of Energy,Office of Science,Office of Basic Energy Sciences,under Contract No.DE-AC02-98CH10886
文摘Liberty State Park in New Jersey,USA,is a ＂brownfield＂ site containing various levels of contaminants.To investigate metal uptake and distributions in plants on the brownfield site,Phragmites australis and Typha latifolia were collected in Liberty State Park during the growing season（May–September）in 2011 at two sites with the high and low metal loads,respectively.The objective of this study was to understand the metal（Fe,Mn,Cu,Pb and Zn）concentration and spatial distributions in P.australis and T.latifolia root systems with micro-meter scale resolution using synchrotron X-ray microfluorescence（μXRF）and synchrotron X-ray computed microtomography（μCMT）techniques.The root structure measurement by synchrotron μCMT showed that high X-ray attenuation substance appeared in the epidermis.Synchrotron μXRF measurement showed that metal concentrations and distributions in the root cross-section between epidermis and vascular tissue were statistically different.Significant correlations were found between metals（Cu,Mn,Pb and Zn）and Fe in the epidermis,implying that metals were scavenged by Fe oxides.The results from this study suggest that the expression of metal transport and accumulation within the root systems may be element specific.The information derived from this study can improve our current knowledge of the wetland plant ecological function in brownfield remediation.
文摘The aim of this study was to determine the species composition, biodiversity and, relative abundance of epiphytic algae and their relationship with environmental variables on Typha latifolia and Phragmites australis at Lake Gala(National Park). Epiphytic algae were gathered monthly by collecting aquatic plants between March 2014 and November 2014. In the epiphytic flora were a total of 133 taxa were identified, 107 taxa were identified on T. latifolia and 96 were discovered on P. australis. While the mean species richness, species diversity and evenness values of the algae identified on T. latifolia were 46, 1.85 and 0.51 respectively, these values were respectively 43, 1.51 and 0.43 on P. australis. While diatoms were generally dominant, other dominant groups in the epiphytic flora included green algae and blue-green algae. The algae that had the highest relative biovolume on T. latifolia were Spirogyra affinis, Oscillatoria sancta and Gomphonema acuminatum, while the algae that had the highest relative biovolume on P. australis were E pithemia adnata, Oscillatoria sancta and R hopalodia gibba. Results show that species composition of epiphytic algae was different, but diversity values were similar on all the macrophytes. The hydrological pulse is one of the most important factors determining the physical and chemical environment of the epiphytic algal community. It was found that some environmental factors were highly eff ective on community distribution in the epiphyton. Additionally, it was observed that some epiphytic algae species had a substrate preference between T. latifolia and P. australis.
基金financially supported by the State Key Development Program for Basic Research of China(973 Program,Grant No.2013CB429904)the National Natural Science Foundation of China(Grant No.91125029)
文摘Reeds are widely distributed in drought and high salt conditions of northwestern China. Leaf epidermal micromorphology, anatomy, chloroplast ultrastructure and physio-chemical characteristics due to long-term adaptation in the natural habitats of common reed （Phragmites communis Trin.） contrasted considerably among three different ecotypes： dune reed （DR）, Gobi salt reed （GSR） and swamp reed （SR）. The main objective of the present study is to determine the adapting characteristics of morphology, anatomy and physiological responses of thin roots in DR, GSR and SR. The results show that root length density was higher in SR and few root hairs were observed in DR. Cross-section anatomical features show that each ecotype has an endodermis and exodermis, while cortex thickness and proportion of root cortical aerenchyma and stele in root structure varied among the three ecotypes. The stele and xylem share a larger area in DR compared to GSR and SR. GSR has a large proportion of the cortex with radialized distribution of aerenchyma cells spacing, and the cortex has a peripheral, mechanically stiff ring in the exodermis. SEM and TEM microscope images show that GSR has a scle- renchyma ring with high lignification in the exodermis. The physio-chemical parameters show that GSR had a higher level of stress tolerance than DR. These findings indicate that developed water-absorbing tissues were largely distributed in the root structure of DR, and a main framework with supporting function spacing with aerenchyma was dominant in GSR in the long term adaptation to their natural habitats, respectively.
基金jointly funded by the Marine S&T Fund of Shandong Province for the Pilot National Laboratory for Marine Science and Technology (Qingdao)(2022QNLM 040003-3)the National Key R&D Program of China (2016YFE0109600)+3 种基金National Natural Science Foundation of China (U22A20558, 41240022, 41876057, 40872167, 41602143)China Geological Survey (1212010611402, GZH201200503, and DD20160144)by in-kind support from the Land Carbon ProgramLand Change Science R&D Program of the United States Geological Survey。
文摘Passive-warming, open-top chambers(OTCs) are widely applied for studying the effects of future climate warming on coastal wetlands. In this study, a set of six OTCs were established at a Phragmites wetland located in the Yellow River Delta of Dongying City, China. With data collected through online transmission and in-situ sensors, the attributes and patterns of realized OTCs warming are demonstrated.The authors also quantified the preliminary influence of experimental chamber warming on plant traits.OTCs produced an elevated average air temperature of 0.8°C(relative to controls) during the growing season(June to October) of 2018, and soil temperatures actually decreased by 0.54°C at a depth of 5 cm and 0.46°C at a depth of 30 cm in the OTCs. Variations in diel patterns of warming depend greatly on the heat sources of incoming radiation in the daytime versus soil heat flux at night. Warming effects were often larger during instantaneous analyses and influenced OTCs air temperatures from-2.5°C to 8.3°C dependent on various meteorological conditions at any given time, ranging from cooling influences from vertical heat exchange and vegetation to radiation-associated warming. Night-time temperature depressions in the OTCs were due to the low turbulence inside OTCs and changes in surface soilatmosphere heat transfer. Plant shoot density, basal diameter, and biomass of Phragmites decreased by23.2%, 6.3%, and 34.0%, respectively, under experimental warming versus controls, and plant height increased by 4.3%, reflecting less carbon allocation to stem structures as plants in the OTCs experienced simultaneous wind buffering. While these passive-warming OTCs created the desired warming effects both to the atmosphere and soils, pest damages on the plant leaves and lodging within the OTCs were extensive and serious, creating the need to consider control options for these chambers and the replicated OTCs studies underway in other Chinese Phragmites marshes(Panjin and Yancheng).
