Green fluorescent protein（GFP） plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore（BHQ） for controlling its expression with exact spatiotemporal resolution.In vitro and in vivo experiments clearly verifie...Green fluorescent protein（GFP） plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore（BHQ） for controlling its expression with exact spatiotemporal resolution.In vitro and in vivo experiments clearly verified that,comparing with Bhc caging, the expression level of caged GFP plasmid was dramatically decreased and then efficiently restored after subsequent photolysis.展开更多
Inspired by nature,biomimetic photoregulated ion transporters have attracted great interest because of the spatial and temporal control over ion transport,in which photocontrollable anion transport remains a very chal...Inspired by nature,biomimetic photoregulated ion transporters have attracted great interest because of the spatial and temporal control over ion transport,in which photocontrollable anion transport remains a very challenging task.Herein,we demonstrate an anionophore AZC for photoregulating anion transport with a high selectivity for chloride over hydroxide across the lipid membranes.Our strategy is based on a well-designed bidentate iodo-triazole anion receptor equipped with an azobenzene moiety,which recognizes Cl^(-)by C-I halogen bonding and C-H hydrogen bonding and switches its states in the lipid membranes from inactive aggregates to active single molecules through azobenzene photoisomerization,thereby achieving controllable Cl^(-)transport.Compared with trans-AZC,cis-AZC exhibits much higher ion transport activity with an EC_(50)(the effective concentration required for 50%activity)value at 28 n M,which is one of the highest reported activities to date for halogen bonding anionophores.Finally,it allows the modulation of Cl^(-)transport upon reversible photoisomerization of AZC using alternative ultraviolet and visible light irradiation.This work provides a new strategy for designing stimuli-responsive anion transporters and paves the way for potential applications in remotely controlled membrane transport and treatment of related diseases.展开更多
Hormogonium,which was thought to play an important role in the dispersal and survival of these microorganisms in their natural habitats,is a distinguishable developmental stage of heterocystous cyanobacteria.The prese...Hormogonium,which was thought to play an important role in the dispersal and survival of these microorganisms in their natural habitats,is a distinguishable developmental stage of heterocystous cyanobacteria.The present study examined the effects of different light conditions and sugars on the differentiation ofNostoc sphaeroides Kiitzing to the hormogonia stage.Results showed that differentiation of hormogonia was light dependent in the absence of sugar,but that close to 100%of cyanobacteria differentiated to hormogonia in the presence of glucose or sucrose,irrespective of the light conditions.This differentiation was inhibited,even in the presence of sugars,upon application of an inhibitor of respiration.Following the testing of different sugars,the effects of different lights were examined.It was found that 5-10μmol·m^-2·s^-1 photon flux density was optimal for hormogonia differentiation.One hundred percent differentiation was obtained with white light irradiation,in contrast with irradiation with green light(80%differentiation)and red light(0-10%differentiation).Although they showed different efficiencies in inducing hormogonia differentiation in N.sphaeroides,the green and red radiation did not display antagonistic effects.When the additional aspect of time dependence was investigated through the application of different fight radiations and an inhibitor of protein synthesis,it was found that the initial 6 h of the differentiation process was crucial for hormogonia differentiation.Taken together,these results show that hormogonia differentiation in N.sphaeroides is either a photoregulated or an energy deoendent orocess.展开更多
The exploration of low-cost and metal-free nanozymes with oxidase-mimicking activity is highly desired due to their attractive properties and potential applications.However,it is still challenging and remains unexploi...The exploration of low-cost and metal-free nanozymes with oxidase-mimicking activity is highly desired due to their attractive properties and potential applications.However,it is still challenging and remains unexploited to fully realize oxidase-like nanozyme in the emerging covalent organic frameworks(COFs)due to their polymeric nature and weak photoelectric activity.We herein report the first example of the preparation and oxidase-mimicking activity of novel ultrathin two-dimensional(2D)COF(termed as TTPA-COF)nanosheets.The ultrathin TTPA-COF nanosheets with hexagonal layered structure are constructed from two flexible photoactive(diarylamino)benzene-based linkers,and exhibit remarkable catalytic activity toward the oxidation of 3,3',5,5'-tetramethylbenzidine(TMB)in the presence of O_(2) due to their large specific surface areas and abundant active sites.Moreover,it is worth noting that the nanozyme activity could be regulated by external light irradiation.Based on the oxidasemimicking activity of TTPA-COF nanosheets,a green colorimetric sensor is proposed for the sensitive and selective determination of glutathione(GSH)in a wide linear range of 0.5–40μM with a detection limit of 0.5μM.This work reported here would open new avenues for the exploration of low-cost and high-efficiency nanozymes,as well as extend the application of 2D COF nanosheets in the fields of catalysis and sensing.展开更多
基金supported by the National Natural Science Foundation of China(No90713009)
文摘Green fluorescent protein（GFP） plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore（BHQ） for controlling its expression with exact spatiotemporal resolution.In vitro and in vivo experiments clearly verified that,comparing with Bhc caging, the expression level of caged GFP plasmid was dramatically decreased and then efficiently restored after subsequent photolysis.
基金supported by the National Natural Science Foundation of China(22171085,22472057)Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism(Shanghai Municipal Education Commission,Grant 2021 Sci&Tech 0328)。
文摘Inspired by nature,biomimetic photoregulated ion transporters have attracted great interest because of the spatial and temporal control over ion transport,in which photocontrollable anion transport remains a very challenging task.Herein,we demonstrate an anionophore AZC for photoregulating anion transport with a high selectivity for chloride over hydroxide across the lipid membranes.Our strategy is based on a well-designed bidentate iodo-triazole anion receptor equipped with an azobenzene moiety,which recognizes Cl^(-)by C-I halogen bonding and C-H hydrogen bonding and switches its states in the lipid membranes from inactive aggregates to active single molecules through azobenzene photoisomerization,thereby achieving controllable Cl^(-)transport.Compared with trans-AZC,cis-AZC exhibits much higher ion transport activity with an EC_(50)(the effective concentration required for 50%activity)value at 28 n M,which is one of the highest reported activities to date for halogen bonding anionophores.Finally,it allows the modulation of Cl^(-)transport upon reversible photoisomerization of AZC using alternative ultraviolet and visible light irradiation.This work provides a new strategy for designing stimuli-responsive anion transporters and paves the way for potential applications in remotely controlled membrane transport and treatment of related diseases.
基金Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(KZCX1-SW-12)the State Key Basic Research and Development Plan of China(2OO2CB4123O6)the Hi-tech Research and Development(863)Program of China(2OO2AA6O1O13).
文摘Hormogonium,which was thought to play an important role in the dispersal and survival of these microorganisms in their natural habitats,is a distinguishable developmental stage of heterocystous cyanobacteria.The present study examined the effects of different light conditions and sugars on the differentiation ofNostoc sphaeroides Kiitzing to the hormogonia stage.Results showed that differentiation of hormogonia was light dependent in the absence of sugar,but that close to 100%of cyanobacteria differentiated to hormogonia in the presence of glucose or sucrose,irrespective of the light conditions.This differentiation was inhibited,even in the presence of sugars,upon application of an inhibitor of respiration.Following the testing of different sugars,the effects of different lights were examined.It was found that 5-10μmol·m^-2·s^-1 photon flux density was optimal for hormogonia differentiation.One hundred percent differentiation was obtained with white light irradiation,in contrast with irradiation with green light(80%differentiation)and red light(0-10%differentiation).Although they showed different efficiencies in inducing hormogonia differentiation in N.sphaeroides,the green and red radiation did not display antagonistic effects.When the additional aspect of time dependence was investigated through the application of different fight radiations and an inhibitor of protein synthesis,it was found that the initial 6 h of the differentiation process was crucial for hormogonia differentiation.Taken together,these results show that hormogonia differentiation in N.sphaeroides is either a photoregulated or an energy deoendent orocess.
基金supported by the financial support from the National Natural Science Foundation of China(NSFC)(Nos.21976166,22006122,and 21405144)the research funding of“Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang”(No.2020R01002)+4 种基金the Class D of Qianjiang Talent Program(No.ZD20011250001)the Science Challenge Project(No.TZ2016004)he Project of State Key Laboratory of Environment-friendly Energy Materials(No.18zd320303)the Scientific Research Starting Foundation for Returned Overseas Chinese Scholars of Sichuan Province(No.19zd3200)support from the Opening Project of Jiangsu Province Engineering Research Center of Agricultural Breeding Pollution Control and Resource(No.2021ABPCR004).
文摘The exploration of low-cost and metal-free nanozymes with oxidase-mimicking activity is highly desired due to their attractive properties and potential applications.However,it is still challenging and remains unexploited to fully realize oxidase-like nanozyme in the emerging covalent organic frameworks(COFs)due to their polymeric nature and weak photoelectric activity.We herein report the first example of the preparation and oxidase-mimicking activity of novel ultrathin two-dimensional(2D)COF(termed as TTPA-COF)nanosheets.The ultrathin TTPA-COF nanosheets with hexagonal layered structure are constructed from two flexible photoactive(diarylamino)benzene-based linkers,and exhibit remarkable catalytic activity toward the oxidation of 3,3',5,5'-tetramethylbenzidine(TMB)in the presence of O_(2) due to their large specific surface areas and abundant active sites.Moreover,it is worth noting that the nanozyme activity could be regulated by external light irradiation.Based on the oxidasemimicking activity of TTPA-COF nanosheets,a green colorimetric sensor is proposed for the sensitive and selective determination of glutathione(GSH)in a wide linear range of 0.5–40μM with a detection limit of 0.5μM.This work reported here would open new avenues for the exploration of low-cost and high-efficiency nanozymes,as well as extend the application of 2D COF nanosheets in the fields of catalysis and sensing.
