Evidence for the long-term optical curve variability and colour behaviour of the BL Lac object Mkn 421 is presented. Our results show that the amplitude of the optical variations of Mkn 421 is only about △B=4.7 magni...Evidence for the long-term optical curve variability and colour behaviour of the BL Lac object Mkn 421 is presented. Our results show that the amplitude of the optical variations of Mkn 421 is only about △B=4.7 magnitude in the B-band for its photometric history from 1899 to 2002. The results of optical photometric monitoring of the Mkn 421 from April 2000 to Jan 2002 are provided. During our observation, Mkn 421 shows significant rapid variations and exhibits short time variability of 2.42 hours in the B band. A strong correlation between the B - V colour index and the magnitude in the B band is found. Our charge coupled device （CCD） photometry of Mkn 421 shows that the measured results are in good agreement with the predicted optical variability period of about 23 years.展开更多
Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivi...Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivity, information transmission, and physiological functions of neural circuits in vivo. However, current techniques for optogenetic stimulation and neuronal activity recording mostly operate independently. Here, we report an all-fiber-transmission photometry system for simultaneous optogenetic manipulation and multi-color recording of neuronal activities and the neurotransmitter release in a freely moving animal. We have designed and manufactured a wavelength-independent multi-branch fiber bundle to enable simultaneous optogenetic manipulation and multi-color recording at different wavelengths. Further, we combine a laser of narrow linewidth with the lock-in amplification method to suppress the optogenetic stimulation-induced artifacts and channel crosstalk. We show that the collection efficiency of our system outperforms a traditional epi-fluorescence system. Further, we demonstrate successful recording of dynamic dopamine(DA) responses to unexpected rewards in the nucleus accumbens(NAc) in a freely moving mouse. We also show simultaneous dual-color recording of neuronal Ca2+ signals and DA dynamics in the NAc upon delivering an unexpected reward and the simultaneous optogenetic activating at dopaminergic terminals in the same location. Thus, our multi-function fiber photometry system provides a compatible, efficient, and flexible solution for neuroscientists to study neural circuits and neurological diseases.展开更多
In vito fber photometry is a powerful technique to analyze the dy namics of population neurons during fiunctional study of neuroscience.Here,we introduced a detailed protocol for fiber photometry-based calciun reordin...In vito fber photometry is a powerful technique to analyze the dy namics of population neurons during fiunctional study of neuroscience.Here,we introduced a detailed protocol for fiber photometry-based calciun reording in freely moving mice,covering from virus injection,fiber stub insertion,optogenetical stimulation to data procurement and analysis.Furthemnore,we applied this protocol to explore neuronal activity of mice latenal-posterior(LP)thalaric nucleus in response to optogenetical stimulation of primary visual cortex(V1)neurons,and explore axon clusters activity of optogenetically evoked V1 neurons.Final confirmation of virus-based protein expression in V1 and precise fber insertion indicated that the surgery procedure of this protocol is reliable for functional calcium recording.The scripts for data analysis and some tips in our protocol are provided in details.Together,this protocol is simple,low-cost,and effective for neuronal activity detection by fiber photometry,which will hep neuroscience researchers to carry out fiunctional and behavioral study in vivo.展开更多
In the central nervous system,the A6 noradrenaline(NA)and the B3 serotonin(5-HT)cell groups are well-recognized players in the descending antinociceptive system,while other NA/5-HT cell groups are not well characteriz...In the central nervous system,the A6 noradrenaline(NA)and the B3 serotonin(5-HT)cell groups are well-recognized players in the descending antinociceptive system,while other NA/5-HT cell groups are not well characterized.A5/A7 NA and B25-HT cells project to the spinal horn and form descending pathways.We recorded G-Ca MP6 green fluorescence signal intensities in the A5/A7 NA and the B25-HT cell groups of awake mice in response to acute tail pinch stimuli,acute heat stimuli,and in the context of a non-noxious control test,using fiber photometry with a calcium imaging system.We first introduced G-Ca MP6 in the A5/A7 NA or B25-HT neuronal soma,using transgenic mice carrying the tetracycline-controlled transactivator transgene under the control of either a dopamineβ-hydroxylase or a tryptophan hydroxylase-2 promoters and by the site-specific injection of adeno-associated virus(AAV-Tet O(3 G)-G-Ca MP6).After confirming the specific expression patterns of G-Ca MP6,we recorded G-Ca MP6 green fluorescence signals in these sites in awake mice in response to acute nociceptive stimuli.G-Ca MP6 fluorescence intensity in the A5,A7,and B2 cell groups was rapidly increased in response to acute nociceptive stimuli and soon after,it returned to baseline fluorescence intensity.This was not observed in the non-noxious control test.The results indicate that acute nociceptive stimuli rapidly increase the activities of A5/A7 NA or B25-HT neurons but the non-noxious stimuli do not.The present study suggests that A5/A7 NA or B25-HT neurons play important roles in nociceptive processing in the central nervous system.We suggest that A5/A7/B2 neurons may be new therapeutic targets.All performed procedures were approved by the Institutional Animal Use Committee of Kagoshima University(MD17105)on February 22,2018.展开更多
Based upon broad-band CCD observation data of comet Hale-Bopp obtained by a 60cm telescope at Xinglong Station of Beijing Astronomical Observatory during March-August, 1996, the photometric results of comet Hale-Bopp ...Based upon broad-band CCD observation data of comet Hale-Bopp obtained by a 60cm telescope at Xinglong Station of Beijing Astronomical Observatory during March-August, 1996, the photometric results of comet Hale-Bopp are reported. Using the photometric results, the dust production rate, radius and mass of nucleus of comet Hale-Bopp are estimated as follows: dM/dt～1.5×10 5 kgs -1, R n～35.5km, and M n～5.65×10 16kg.展开更多
Because of the 3D nature of galaxies, an algorithm for constructing spatial density distribution models of galaxies on the basis of galaxy images has many advan- tages over approximations of the surface density distri...Because of the 3D nature of galaxies, an algorithm for constructing spatial density distribution models of galaxies on the basis of galaxy images has many advan- tages over approximations of the surface density distribution. We present a method for deriving the spatial structure and overall parameters of galaxies from images and estimate its accuracy and derived parameter degeneracies on a sample of idealised model galaxies. The test galaxies consist component with varying proportions and of a disc-like component and a spheroidal properties. Both components are assumed to be axially symmetric and coplanar. We simulate these test galaxies as if they had been observed in the SDSS project through ugriz filters, thus gaining a set of realis- tically imperfect images of galaxies with known intrinsic properties. These artificial SDSS galaxies were thereafter remodelled by approximating the surface brightness distribution with a 2D projection of a bulge＋disc spatial distribution model and the restored parameters were compared to the initial ones. Down to the r-band limiting magnitude of 18, errors in the restored integral luminosities and colour indices re- main within 0.05 mag and errors in the luminosities of individual components within 0.2 mag. Accuracy of the restored bulge-to-disc luminosity ratio （B/D） is within 40% in most cases, and becomes worse for galaxies with low B/D, but the general balance between bulges and discs is not shifted systematically. Assuming that the intrinsic disc axial ratio is ≤ 0.3, then the inclination angles can be estimated with errors 〈 5° for most of the galaxies with B/D 〈 2 and with errors 〈 15° up to B/D = 6. Errors in the recovered sizes of the galactic components are below 10% in most cases. The axial ratios and the shape parameter N of Einasto＇s distribution （similar to the Sersic index） are relatively inaccurate, but can provide statistical estimates for large samples. In general, models of disc components are more accurate than models of spheroidal components for geometrical reasons.展开更多
In this paper, surface photometry and dynamical properties of Lenticular galaxies will be developed and applied to NGC3245. In this respect, we established new relation between the intensity distribution I and the sem...In this paper, surface photometry and dynamical properties of Lenticular galaxies will be developed and applied to NGC3245. In this respect, we established new relation between the intensity distribution I and the semi-major axis a Moreover, some basic statistics of both independent and the dependent variables of the relation are also given. In addition to the I(a) relation , the Sérsic r1/n model is applied for the intensity profile I(r) resulting in an estimation of the effective radius, re, and the surface brightness it encloses, μe. Both relations (I(a) and I(r)) are accurate as judged by the precision criteria which are: the probable errors for the coefficients , the estimated variance of the fit and the Q value (the square distance between the exact solution and the least square estimated solution) where all very satisfactory. Correlation coefficients between some parameters of the isophotes are also computed. Finally as examples of applications of surface photometry we determined the dynamical properties: mass, density, potential distributions, as well as distributions of escape and circular speeds in terms of Sérsic model.展开更多
We present UBVI surface photometry over a 20.5' × 20.5' area of the late-type spiral galaxy NGC 300. We have derived isophotal maps, surface brightness profiles, ellipticity profiles, position angle profi...We present UBVI surface photometry over a 20.5' × 20.5' area of the late-type spiral galaxy NGC 300. We have derived isophotal maps, surface brightness profiles, ellipticity profiles, position angle profiles, and color profiles. By merging our I-band measurements with those of Boker et al. based on Hubble Space Telescope observations, we have obtained combined I-band surface brightness profiles for the region 0.02' < r < 500' and have decomposed the profiles into three components: a nucleus, a bulge, and an exponential disk.展开更多
Dome A,in Antarctica,offers an exceptional site for ground-based infrared astronomy,with its extremely low atmospheric infrared background noise and excellent seeing conditions.However,deploying near-infrared telescop...Dome A,in Antarctica,offers an exceptional site for ground-based infrared astronomy,with its extremely low atmospheric infrared background noise and excellent seeing conditions.However,deploying near-infrared telescopes in the harsh environment of Antarctica faces the critical challenge of frost accumulation on optical mirrors.While indium tin oxide heating films effectively defrost visible-band Antarctic astronomical telescopes,their thermal radiation at infrared wavelengths introduces significant stray light,severely degrading the signal-to-noise ratio for infrared observations.To address this limitation,we have designed a mechanical snow-removal system capable of efficiently clearing frost from sealing window surfaces at temperatures as low as–80°C.Aperture photometry of target sources,Canopus and HD 2151,revealed that after six days without intervention,floating snow extinction reduced target brightness by up to 3 magnitudes.Following mechanical defrosting,the source flux recovered to stable levels,with measured magnitudes showing rapid initial improvement followed by stabilization.Data analysis indicates that a frost removal strategy operating every 48 h,with each operation consisting of 4–6 cycles,enables efficient removal of frost and snow without introducing additional thermal noise.Future work will focus on optimizing the adaptive control algorithm and exploring novel low-temperature defrosting materials to extend the periods during which Antarctic infrared telescopes can operate unattended.展开更多
The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain functio...The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with emotions.Specifically, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal mice.After knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was obviously mitigated. Additionally, administration of an excitatory amino acid transporter 2 inhibitor in the basolateral amygdala yielded a notable reduction in anxiety level among mice subjected to stress. These results suggest that basolateral amygdala astrocytic excitatory amino acid transporter 2 plays a role in in the regulation of unpredictable chronic mild stress-induced anxiety-like behavior by impacting the activity of local glutamatergic neurons, and targeting excitatory amino acid transporter 2 in the basolateral amygdala holds therapeutic promise for addressing anxiety disorders.展开更多
This paper presents a comprehensive analysis of the photometric system of the University of Chinese Academy of Sciences 70 cm Telescope located at the Yan-qi Lake campus of the University of Chinese Academy of Science...This paper presents a comprehensive analysis of the photometric system of the University of Chinese Academy of Sciences 70 cm Telescope located at the Yan-qi Lake campus of the University of Chinese Academy of Sciences.We evaluated the linearity,bias stability,and dark current of the camera.Utilizing the Johnson-Cousins Blue-Visible-Red-Infrared filter system and an Andor DZ936 charge-coupled device camera,we conducted extensive observations of Landolt standard stars to determine the color terms,atmospheric extinction coefficients,photometric zero-points,and the sky background brightness.The results indicate that this telescope demonstrates excellent performance in photometric calibration and good system performance overall,meeting the requirements for limited scientific research and teaching purposes.展开更多
Light emitting diode(LED)lighting is becoming more and more popular,as incandescent lamps are being phased out globally.LEDs have several advantages over incandescent lamps,including energy efficiency,robustness,long ...Light emitting diode(LED)lighting is becoming more and more popular,as incandescent lamps are being phased out globally.LEDs have several advantages over incandescent lamps,including energy efficiency,robustness,long lifetime,and good temporal stability.The three latter features make LEDs attractive candidates as new photometric standards.Because the spectra of white LEDs are limited to the visible wavelength range,a novel method for the realization of photometric units based on the predictable quantum efficient detector(PQED)can be utilized.The method eliminates the need of photometric filters that are traditionally used in photometry,and instead relies on carrying out the photometric weighting numerically based on the measured relative spectrum of the source.The PQED-based realization simplifies the traceability chain of photometric measurements significantly as compared with the traditional filter-based method.The measured illuminance values of a white LED deviate by only 0.03%when determined by the new and the traditional methods.The new PQED method has significantly lower expanded uncertainty of 0.26%(k=52)as compared with that of the traditional filter-based method of 0.42%(k=52).Furthermore,when filtered photometers that measure LED lighting are calibrated using LED lamps as calibration sources instead of incandescent lamps,a significant decrease in the uncertainty related to the spectral mismatch correction can be obtained.The maximum spectral mismatch errors of LED measurements decreased on average by a factor of 3 when switching from an incandescent lamp to an LED calibration source.展开更多
Based upon broad-band CCD observation data of comet Hale-Bopp obtained by a 60 cm telescope at Xinglong Station of Beijing Astronomical Observatory during March-August, 1996, the photometric results of comet Hale-Bopp...Based upon broad-band CCD observation data of comet Hale-Bopp obtained by a 60 cm telescope at Xinglong Station of Beijing Astronomical Observatory during March-August, 1996, the photometric results of comet Hale-Bopp are reported. Using the photometric results, the dust production rate, radius and mass of nucleus of comet Hale-Bopp are estimated as follows: dM/dt × 1.5 × 105 kgs?1, Rn × 35.5 km, and Mn × 5.65 × 1016 kg.展开更多
We present a study of star-forming galaxies (SFGs) with pseudobulges (bulges with Sérsic index n<2) in a loca close major-merger galaxy pair sample (H-KPAIR).With data from new aperture photometries in the opt...We present a study of star-forming galaxies (SFGs) with pseudobulges (bulges with Sérsic index n<2) in a loca close major-merger galaxy pair sample (H-KPAIR).With data from new aperture photometries in the optical and near-infrared bands (aperture size of 7 kpc) and from the literature,we find that the mean Age of central stellar populations in Spirals with pseudobulges is consistent with that of disky galaxies and is nearly constant against the bulge-to-total ratio (B/T).Paired Spirals have a slightly lower fraction of pure disk galaxies (B/T≤0.1) than their counterparts in the control sample.Compared to SFGs with classical bulges,those with pseudobulges have a higher (>2σ) mean of specific star formation rate (sSFR) enhancement (sSFR_(enh)=0.33±0.07 versus sSFR_(enh)=0.12±0.06) and broader scatter (by~1 dex).The eight SFGs that have the highest sSFR_(enh)in the sample all have pseudobulges.A majority (69%) of paired SFGs with strong enhancement (having sSFR more than5 times the median of the control galaxies) have pseudobulges.The Spitzer data show that the pseudobulges in these galaxies are tightly linked to nuclear/circum-nuclear starbursts.Pseudobulge SFGs in S+S and in S+E pairs have significantly (>3σ) different sSFR enhancement,with the means of sSFR_(enh)=0.45±0.08 and-0.04±0.11respectively.We find a decrease in the sSFR enhancements with the density of the environment for SFGs with pseudobulges.Since a high fraction (5/11) of pseudobulge SFGs in S+E pairs are in rich groups/clusters (loca density N_(1Mpc)≥7),the dense environment might be the cause for their low s SFR_(enh).展开更多
Mitochondrial membrane potential(MMP)plays a crucial role in the function of cells and organelles,involving various cellular physiological processes,including energy production,formation of reactive oxygen species(ROS...Mitochondrial membrane potential(MMP)plays a crucial role in the function of cells and organelles,involving various cellular physiological processes,including energy production,formation of reactive oxygen species(ROS),unfolded protein stress,and cell survival.Currently,there is a lack of genetically encoded fluorescence indicators(GEVIs)for MMP.In our screening of various GEVIs for their potential monitoring MMP,the Accelerated Sensor of Action Potentials(ASAP)demonstrated optimal performance in targeting mitochondria and sensitivity to depolarization in multiple cell types.However,mitochondrial ASAPs also displayed sensitivity to ROS in cardiomyocytes.Therefore,two ASAP mutants resistant to ROS were generated.A double mutant ASAP3-ST exhibited the highest voltage sensitivity but weaker fluorescence.Overall,four GEVIs capable of targeting mitochondria were obtained and named mitochondrial potential indicators 1-4(MPI-1-4).In vivo,fiber photometry experiments utilizing MPI-2 revealed a mitochondrial depolarization during isoflurane-induced narcosis in the M2 cortex.展开更多
A survey was carried out with the aim of assessing the level of the total phenolic compounds （TPC）, essential （Na, K, Ca, Mg, Cu, Zn） and toxic （Cd and Pb） elements in 10 Latvian whole grain rye and/or wheat flo...A survey was carried out with the aim of assessing the level of the total phenolic compounds （TPC）, essential （Na, K, Ca, Mg, Cu, Zn） and toxic （Cd and Pb） elements in 10 Latvian whole grain rye and/or wheat flour bread samples, obtained from the local market. The quantities of the TPC were determined by spectrophotometry. Mineral content was determined by flame photometry, flame atom absorption spectrometry （FAAS） and electrothermal atomic absorption spectrometry （ETAA） after wet digestion in concentrated HNO3. The values were comparable to the literature data. The average content in all the samples was （on a basis of weight of the product） 547 ± 28 mg/100 g for Na; 280 ±20 mg/100 g for K; 32 ± 2 mg/100 g for Ca; 88 ±5 mg/100 g for Mg; 0.49 ± 0.09 mg/100 g for Cu; 1.6 ±0.2 mg/100 g for Zn. Both Pb and Cd were determined below the limit of detection （LOD 5 0.10 mg/100 g）. The LOD values were determined for each element. The TPC content in defatted samples was slightly lower than the non-defatted ones, showing the dependence on the sample preparation. The calculated average levels of the elements were compared with the maximum levels recommended or regulated by the national legislation.展开更多
This is a prospective interventional clinical study evaluating intraocular inflammation developed after Ultrasound Cyclo Plasty(UCP) for the treatment of glaucoma. Eighteen eyes of 18 patients were treated with UCP se...This is a prospective interventional clinical study evaluating intraocular inflammation developed after Ultrasound Cyclo Plasty(UCP) for the treatment of glaucoma. Eighteen eyes of 18 patients were treated with UCP second-generation probes(Eye OP1). After treatment, the mean intraocular pressure(IOP) significantly decreased from 26.8±7.2 to 18.8±6.1 mm Hg at day 1 and to 14.7±3.4 mm Hg at month 6(all P<0.001). Mean laser flare-cell photometry value steeply increased after surgery from 12.1±7.5 to 64.1±53.9 ph/ms(P=0.001) at day 1, and then progressively decreased to respectively 60.6±49.7 at day 7, 43.5±38.5 at day 14 and 28.2±18.3 at month 1(all P<0.05), returning at levels similar to baseline ones at month 3 and month 6(respectively 16.7±6.2 and 12.8±10.2, both P>0.05). A significant negative correlation was found between postoperative increase of aqueous flare values and anterior chamber depth(R=-0.568, P=0.014). This timeframe may be considered reasonable for repeating UCP treatment, when required.展开更多
Deep brain stimulation of the subthalamic nucleus(STN)is an effective therapy for motor deficits in Parkinson’s disease(PD),but commonly causes weight gain in late-phase PD patients probably by increasing feeding mot...Deep brain stimulation of the subthalamic nucleus(STN)is an effective therapy for motor deficits in Parkinson’s disease(PD),but commonly causes weight gain in late-phase PD patients probably by increasing feeding motivation.It is unclear how STN neurons represent and modulate feeding behavior in different internal states.In the present study,we found that feeding caused a robust activation of STN neurons in mice(GCaMP6 signal increased by 48.4%±7.2%,n=9,P=0.0003),and the extent varied with the size,valence,and palatability of food,but not with the repetition of feeding.Interestingly,energy deprivation increased the spontaneous firing rate(8.5±1.5 Hz,n=17,versus 4.7±0.7 Hz,n=18,P=0.03)and the depolarization-induced spikes in STN neurons,as well as enhanced the STN responses to feeding.Optogenetic experiments revealed that stimulation and inhibition of STN neurons respectively reduced(by 11%±6%,n=6,P=0.02)and enhanced(by 36%±15%,n=7,P=0.03)food intake only in the dark phase.In conclusion,our results support the hypothesis that STN neurons are activated by feeding behavior,depending on energy homeostatic status and the palatability of food,and modulation of these neurons is sufficient to regulate food intake.展开更多
基金Project supported by an CRCG grant of the University of Hong Kong, Yunnan Provincial Foundation of Science of China （Grant No 2004A0027M） and the National Natural Science Foundation of China（Grant No 10363002）, and the Natural Science Foundation of China Education Department（Grant No 02142）.
文摘Evidence for the long-term optical curve variability and colour behaviour of the BL Lac object Mkn 421 is presented. Our results show that the amplitude of the optical variations of Mkn 421 is only about △B=4.7 magnitude in the B-band for its photometric history from 1899 to 2002. The results of optical photometric monitoring of the Mkn 421 from April 2000 to Jan 2002 are provided. During our observation, Mkn 421 shows significant rapid variations and exhibits short time variability of 2.42 hours in the B band. A strong correlation between the B - V colour index and the magnitude in the B band is found. Our charge coupled device （CCD） photometry of Mkn 421 shows that the measured results are in good agreement with the predicted optical variability period of about 23 years.
基金supported by Beijing Municipal Governmentsupported by the National Natural Science Foundation of China(Grant Nos.61890952)the Director Fund of WNLO。
文摘Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivity, information transmission, and physiological functions of neural circuits in vivo. However, current techniques for optogenetic stimulation and neuronal activity recording mostly operate independently. Here, we report an all-fiber-transmission photometry system for simultaneous optogenetic manipulation and multi-color recording of neuronal activities and the neurotransmitter release in a freely moving animal. We have designed and manufactured a wavelength-independent multi-branch fiber bundle to enable simultaneous optogenetic manipulation and multi-color recording at different wavelengths. Further, we combine a laser of narrow linewidth with the lock-in amplification method to suppress the optogenetic stimulation-induced artifacts and channel crosstalk. We show that the collection efficiency of our system outperforms a traditional epi-fluorescence system. Further, we demonstrate successful recording of dynamic dopamine(DA) responses to unexpected rewards in the nucleus accumbens(NAc) in a freely moving mouse. We also show simultaneous dual-color recording of neuronal Ca2+ signals and DA dynamics in the NAc upon delivering an unexpected reward and the simultaneous optogenetic activating at dopaminergic terminals in the same location. Thus, our multi-function fiber photometry system provides a compatible, efficient, and flexible solution for neuroscientists to study neural circuits and neurological diseases.
基金supported by the National Natural Science Foundation of China (Grant Nos.31371106 and 91632110)HZAU Independent Innovation Fund (2014BQ019).
文摘In vito fber photometry is a powerful technique to analyze the dy namics of population neurons during fiunctional study of neuroscience.Here,we introduced a detailed protocol for fiber photometry-based calciun reording in freely moving mice,covering from virus injection,fiber stub insertion,optogenetical stimulation to data procurement and analysis.Furthemnore,we applied this protocol to explore neuronal activity of mice latenal-posterior(LP)thalaric nucleus in response to optogenetical stimulation of primary visual cortex(V1)neurons,and explore axon clusters activity of optogenetically evoked V1 neurons.Final confirmation of virus-based protein expression in V1 and precise fber insertion indicated that the surgery procedure of this protocol is reliable for functional calcium recording.The scripts for data analysis and some tips in our protocol are provided in details.Together,this protocol is simple,low-cost,and effective for neuronal activity detection by fiber photometry,which will hep neuroscience researchers to carry out fiunctional and behavioral study in vivo.
基金supported by JSPS KAKENHI grants(Nos.19K17093 to SM20K06858 to AYamashita16H05130 to TK)and CREST JST(No.JPMJCR1656 to AYamanaka)。
文摘In the central nervous system,the A6 noradrenaline(NA)and the B3 serotonin(5-HT)cell groups are well-recognized players in the descending antinociceptive system,while other NA/5-HT cell groups are not well characterized.A5/A7 NA and B25-HT cells project to the spinal horn and form descending pathways.We recorded G-Ca MP6 green fluorescence signal intensities in the A5/A7 NA and the B25-HT cell groups of awake mice in response to acute tail pinch stimuli,acute heat stimuli,and in the context of a non-noxious control test,using fiber photometry with a calcium imaging system.We first introduced G-Ca MP6 in the A5/A7 NA or B25-HT neuronal soma,using transgenic mice carrying the tetracycline-controlled transactivator transgene under the control of either a dopamineβ-hydroxylase or a tryptophan hydroxylase-2 promoters and by the site-specific injection of adeno-associated virus(AAV-Tet O(3 G)-G-Ca MP6).After confirming the specific expression patterns of G-Ca MP6,we recorded G-Ca MP6 green fluorescence signals in these sites in awake mice in response to acute nociceptive stimuli.G-Ca MP6 fluorescence intensity in the A5,A7,and B2 cell groups was rapidly increased in response to acute nociceptive stimuli and soon after,it returned to baseline fluorescence intensity.This was not observed in the non-noxious control test.The results indicate that acute nociceptive stimuli rapidly increase the activities of A5/A7 NA or B25-HT neurons but the non-noxious stimuli do not.The present study suggests that A5/A7 NA or B25-HT neurons play important roles in nociceptive processing in the central nervous system.We suggest that A5/A7/B2 neurons may be new therapeutic targets.All performed procedures were approved by the Institutional Animal Use Committee of Kagoshima University(MD17105)on February 22,2018.
文摘Based upon broad-band CCD observation data of comet Hale-Bopp obtained by a 60cm telescope at Xinglong Station of Beijing Astronomical Observatory during March-August, 1996, the photometric results of comet Hale-Bopp are reported. Using the photometric results, the dust production rate, radius and mass of nucleus of comet Hale-Bopp are estimated as follows: dM/dt～1.5×10 5 kgs -1, R n～35.5km, and M n～5.65×10 16kg.
基金supported by the Estonian Science Foundationprojects IUT26-2 and IUT40-2support by the Centre of Excellence of Dark Matter in(Astro)particle Physics and Cosmology(TK120)+3 种基金Funding for SDSS-Ⅲ has been provided by the Alfred P.Sloan Foundationthe Participating Institutionsthe National Science Foundationthe U.S.Department of Energy Office of Science
文摘Because of the 3D nature of galaxies, an algorithm for constructing spatial density distribution models of galaxies on the basis of galaxy images has many advan- tages over approximations of the surface density distribution. We present a method for deriving the spatial structure and overall parameters of galaxies from images and estimate its accuracy and derived parameter degeneracies on a sample of idealised model galaxies. The test galaxies consist component with varying proportions and of a disc-like component and a spheroidal properties. Both components are assumed to be axially symmetric and coplanar. We simulate these test galaxies as if they had been observed in the SDSS project through ugriz filters, thus gaining a set of realis- tically imperfect images of galaxies with known intrinsic properties. These artificial SDSS galaxies were thereafter remodelled by approximating the surface brightness distribution with a 2D projection of a bulge＋disc spatial distribution model and the restored parameters were compared to the initial ones. Down to the r-band limiting magnitude of 18, errors in the restored integral luminosities and colour indices re- main within 0.05 mag and errors in the luminosities of individual components within 0.2 mag. Accuracy of the restored bulge-to-disc luminosity ratio （B/D） is within 40% in most cases, and becomes worse for galaxies with low B/D, but the general balance between bulges and discs is not shifted systematically. Assuming that the intrinsic disc axial ratio is ≤ 0.3, then the inclination angles can be estimated with errors 〈 5° for most of the galaxies with B/D 〈 2 and with errors 〈 15° up to B/D = 6. Errors in the recovered sizes of the galactic components are below 10% in most cases. The axial ratios and the shape parameter N of Einasto＇s distribution （similar to the Sersic index） are relatively inaccurate, but can provide statistical estimates for large samples. In general, models of disc components are more accurate than models of spheroidal components for geometrical reasons.
文摘In this paper, surface photometry and dynamical properties of Lenticular galaxies will be developed and applied to NGC3245. In this respect, we established new relation between the intensity distribution I and the semi-major axis a Moreover, some basic statistics of both independent and the dependent variables of the relation are also given. In addition to the I(a) relation , the Sérsic r1/n model is applied for the intensity profile I(r) resulting in an estimation of the effective radius, re, and the surface brightness it encloses, μe. Both relations (I(a) and I(r)) are accurate as judged by the precision criteria which are: the probable errors for the coefficients , the estimated variance of the fit and the Q value (the square distance between the exact solution and the least square estimated solution) where all very satisfactory. Correlation coefficients between some parameters of the isophotes are also computed. Finally as examples of applications of surface photometry we determined the dynamical properties: mass, density, potential distributions, as well as distributions of escape and circular speeds in terms of Sérsic model.
文摘We present UBVI surface photometry over a 20.5' × 20.5' area of the late-type spiral galaxy NGC 300. We have derived isophotal maps, surface brightness profiles, ellipticity profiles, position angle profiles, and color profiles. By merging our I-band measurements with those of Boker et al. based on Hubble Space Telescope observations, we have obtained combined I-band surface brightness profiles for the region 0.02' < r < 500' and have decomposed the profiles into three components: a nucleus, a bulge, and an exponential disk.
基金supported by the Space Debris Research Project,China(KJSP2020010102)the National Key R&D Program of China(2022YFC2807300)the National Natural Science Foundation of China(12573081)。
文摘Dome A,in Antarctica,offers an exceptional site for ground-based infrared astronomy,with its extremely low atmospheric infrared background noise and excellent seeing conditions.However,deploying near-infrared telescopes in the harsh environment of Antarctica faces the critical challenge of frost accumulation on optical mirrors.While indium tin oxide heating films effectively defrost visible-band Antarctic astronomical telescopes,their thermal radiation at infrared wavelengths introduces significant stray light,severely degrading the signal-to-noise ratio for infrared observations.To address this limitation,we have designed a mechanical snow-removal system capable of efficiently clearing frost from sealing window surfaces at temperatures as low as–80°C.Aperture photometry of target sources,Canopus and HD 2151,revealed that after six days without intervention,floating snow extinction reduced target brightness by up to 3 magnitudes.Following mechanical defrosting,the source flux recovered to stable levels,with measured magnitudes showing rapid initial improvement followed by stabilization.Data analysis indicates that a frost removal strategy operating every 48 h,with each operation consisting of 4–6 cycles,enables efficient removal of frost and snow without introducing additional thermal noise.Future work will focus on optimizing the adaptive control algorithm and exploring novel low-temperature defrosting materials to extend the periods during which Antarctic infrared telescopes can operate unattended.
基金supported by the National Natural Science Foundation of China,Nos.32371070 (to JT),31761163005 (to JT),32100824 (to QX)the Shenzhen Science and Technology Program,Nos.RCBS20210609104606024 (to QX),JCY20210324101813035 (to DL)+4 种基金the Guangdong Provincial Key S&T Program,No.2018B030336001 (to JT)the Key Basic Research Program of Shenzhen Science and Technology Innovation Commission,Nos.JCYJ20200109115405930 (to JT),JCYJ20220818101615033 (to DL),JCYJ20210324115811031 (to QX),JCYJ20200109150717745 (to QX)Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases,No.ZDSYS20220304163558001 (to JT)Guangdong Provincial Key Laboratory of Brain Connectome and Behavior,No.2023B1212060055 (to JT)the China Postdoctoral Science Foundation,No.2021M693298 (to QX)。
文摘The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with emotions.Specifically, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal mice.After knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was obviously mitigated. Additionally, administration of an excitatory amino acid transporter 2 inhibitor in the basolateral amygdala yielded a notable reduction in anxiety level among mice subjected to stress. These results suggest that basolateral amygdala astrocytic excitatory amino acid transporter 2 plays a role in in the regulation of unpredictable chronic mild stress-induced anxiety-like behavior by impacting the activity of local glutamatergic neurons, and targeting excitatory amino acid transporter 2 in the basolateral amygdala holds therapeutic promise for addressing anxiety disorders.
基金supported by National Key R&D Program of China(2023YFA1609700)Research and Education Integration Funding。
文摘This paper presents a comprehensive analysis of the photometric system of the University of Chinese Academy of Sciences 70 cm Telescope located at the Yan-qi Lake campus of the University of Chinese Academy of Sciences.We evaluated the linearity,bias stability,and dark current of the camera.Utilizing the Johnson-Cousins Blue-Visible-Red-Infrared filter system and an Andor DZ936 charge-coupled device camera,we conducted extensive observations of Landolt standard stars to determine the color terms,atmospheric extinction coefficients,photometric zero-points,and the sky background brightness.The results indicate that this telescope demonstrates excellent performance in photometric calibration and good system performance overall,meeting the requirements for limited scientific research and teaching purposes.
基金The research leading to these results has received partial funding from the European Metrology Research Programme(EMRP)project SIB57‘New Primary Standards and Traceability for Radiometry’The EMRP is jointly funded by the EMRP participating countries within EURAMET and the European Union.
文摘Light emitting diode(LED)lighting is becoming more and more popular,as incandescent lamps are being phased out globally.LEDs have several advantages over incandescent lamps,including energy efficiency,robustness,long lifetime,and good temporal stability.The three latter features make LEDs attractive candidates as new photometric standards.Because the spectra of white LEDs are limited to the visible wavelength range,a novel method for the realization of photometric units based on the predictable quantum efficient detector(PQED)can be utilized.The method eliminates the need of photometric filters that are traditionally used in photometry,and instead relies on carrying out the photometric weighting numerically based on the measured relative spectrum of the source.The PQED-based realization simplifies the traceability chain of photometric measurements significantly as compared with the traditional filter-based method.The measured illuminance values of a white LED deviate by only 0.03%when determined by the new and the traditional methods.The new PQED method has significantly lower expanded uncertainty of 0.26%(k=52)as compared with that of the traditional filter-based method of 0.42%(k=52).Furthermore,when filtered photometers that measure LED lighting are calibrated using LED lamps as calibration sources instead of incandescent lamps,a significant decrease in the uncertainty related to the spectral mismatch correction can be obtained.The maximum spectral mismatch errors of LED measurements decreased on average by a factor of 3 when switching from an incandescent lamp to an LED calibration source.
文摘Based upon broad-band CCD observation data of comet Hale-Bopp obtained by a 60 cm telescope at Xinglong Station of Beijing Astronomical Observatory during March-August, 1996, the photometric results of comet Hale-Bopp are reported. Using the photometric results, the dust production rate, radius and mass of nucleus of comet Hale-Bopp are estimated as follows: dM/dt × 1.5 × 105 kgs?1, Rn × 35.5 km, and Mn × 5.65 × 1016 kg.
基金supported by the National Natural Science Foundation of China(NSFC)No.11873055 and No.11933003sponsored(in part)by the Chinese Academy of Sciences(CAS)through a grant to the CAS South America Center for Astronomy(CASSACA)+4 种基金support from project PID2020-114414GB-100,financed by MCIN/AEI/10.13039/501100011033the Junta de Andaluciaía(Spain)grant FQM108support by the National Key R&D Program of China No.2017YFA0402600the National Natural Science Foundation of China(NSFC)grant Nos.11890692,12133008,and 12221003China Manned Space Project No.CMS-CSST2021-A04。
文摘We present a study of star-forming galaxies (SFGs) with pseudobulges (bulges with Sérsic index n<2) in a loca close major-merger galaxy pair sample (H-KPAIR).With data from new aperture photometries in the optical and near-infrared bands (aperture size of 7 kpc) and from the literature,we find that the mean Age of central stellar populations in Spirals with pseudobulges is consistent with that of disky galaxies and is nearly constant against the bulge-to-total ratio (B/T).Paired Spirals have a slightly lower fraction of pure disk galaxies (B/T≤0.1) than their counterparts in the control sample.Compared to SFGs with classical bulges,those with pseudobulges have a higher (>2σ) mean of specific star formation rate (sSFR) enhancement (sSFR_(enh)=0.33±0.07 versus sSFR_(enh)=0.12±0.06) and broader scatter (by~1 dex).The eight SFGs that have the highest sSFR_(enh)in the sample all have pseudobulges.A majority (69%) of paired SFGs with strong enhancement (having sSFR more than5 times the median of the control galaxies) have pseudobulges.The Spitzer data show that the pseudobulges in these galaxies are tightly linked to nuclear/circum-nuclear starbursts.Pseudobulge SFGs in S+S and in S+E pairs have significantly (>3σ) different sSFR enhancement,with the means of sSFR_(enh)=0.45±0.08 and-0.04±0.11respectively.We find a decrease in the sSFR enhancements with the density of the environment for SFGs with pseudobulges.Since a high fraction (5/11) of pseudobulge SFGs in S+E pairs are in rich groups/clusters (loca density N_(1Mpc)≥7),the dense environment might be the cause for their low s SFR_(enh).
基金supported by the National Natural Science Foundation (NSF)of China:JSK (32071137 and 92054103)Funding for Scientific Research and Innovation Team of The First Affliated Hospital of Zhengzhou University:JSK (ZYCXTD2023014)。
文摘Mitochondrial membrane potential(MMP)plays a crucial role in the function of cells and organelles,involving various cellular physiological processes,including energy production,formation of reactive oxygen species(ROS),unfolded protein stress,and cell survival.Currently,there is a lack of genetically encoded fluorescence indicators(GEVIs)for MMP.In our screening of various GEVIs for their potential monitoring MMP,the Accelerated Sensor of Action Potentials(ASAP)demonstrated optimal performance in targeting mitochondria and sensitivity to depolarization in multiple cell types.However,mitochondrial ASAPs also displayed sensitivity to ROS in cardiomyocytes.Therefore,two ASAP mutants resistant to ROS were generated.A double mutant ASAP3-ST exhibited the highest voltage sensitivity but weaker fluorescence.Overall,four GEVIs capable of targeting mitochondria were obtained and named mitochondrial potential indicators 1-4(MPI-1-4).In vivo,fiber photometry experiments utilizing MPI-2 revealed a mitochondrial depolarization during isoflurane-induced narcosis in the M2 cortex.
文摘A survey was carried out with the aim of assessing the level of the total phenolic compounds （TPC）, essential （Na, K, Ca, Mg, Cu, Zn） and toxic （Cd and Pb） elements in 10 Latvian whole grain rye and/or wheat flour bread samples, obtained from the local market. The quantities of the TPC were determined by spectrophotometry. Mineral content was determined by flame photometry, flame atom absorption spectrometry （FAAS） and electrothermal atomic absorption spectrometry （ETAA） after wet digestion in concentrated HNO3. The values were comparable to the literature data. The average content in all the samples was （on a basis of weight of the product） 547 ± 28 mg/100 g for Na; 280 ±20 mg/100 g for K; 32 ± 2 mg/100 g for Ca; 88 ±5 mg/100 g for Mg; 0.49 ± 0.09 mg/100 g for Cu; 1.6 ±0.2 mg/100 g for Zn. Both Pb and Cd were determined below the limit of detection （LOD 5 0.10 mg/100 g）. The LOD values were determined for each element. The TPC content in defatted samples was slightly lower than the non-defatted ones, showing the dependence on the sample preparation. The calculated average levels of the elements were compared with the maximum levels recommended or regulated by the national legislation.
文摘This is a prospective interventional clinical study evaluating intraocular inflammation developed after Ultrasound Cyclo Plasty(UCP) for the treatment of glaucoma. Eighteen eyes of 18 patients were treated with UCP second-generation probes(Eye OP1). After treatment, the mean intraocular pressure(IOP) significantly decreased from 26.8±7.2 to 18.8±6.1 mm Hg at day 1 and to 14.7±3.4 mm Hg at month 6(all P<0.001). Mean laser flare-cell photometry value steeply increased after surgery from 12.1±7.5 to 64.1±53.9 ph/ms(P=0.001) at day 1, and then progressively decreased to respectively 60.6±49.7 at day 7, 43.5±38.5 at day 14 and 28.2±18.3 at month 1(all P<0.05), returning at levels similar to baseline ones at month 3 and month 6(respectively 16.7±6.2 and 12.8±10.2, both P>0.05). A significant negative correlation was found between postoperative increase of aqueous flare values and anterior chamber depth(R=-0.568, P=0.014). This timeframe may be considered reasonable for repeating UCP treatment, when required.
基金the National Natural Science Foundation of China(81701100,81870891,and 81971038)the Fund for Jiangsu Province Specially-Appointed Professor(2016 and 2018)+3 种基金the Natural Science Foundation of Jiangsu Province,China(BK20171160)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(17KJA320007 and 18KJA320009)the Jiangsu Province Fund for Dominant Discipline(Anesthesiology)Academic Startup Packages from Xuzhou Medical University,China(D2017009 and D2017010)。
文摘Deep brain stimulation of the subthalamic nucleus(STN)is an effective therapy for motor deficits in Parkinson’s disease(PD),but commonly causes weight gain in late-phase PD patients probably by increasing feeding motivation.It is unclear how STN neurons represent and modulate feeding behavior in different internal states.In the present study,we found that feeding caused a robust activation of STN neurons in mice(GCaMP6 signal increased by 48.4%±7.2%,n=9,P=0.0003),and the extent varied with the size,valence,and palatability of food,but not with the repetition of feeding.Interestingly,energy deprivation increased the spontaneous firing rate(8.5±1.5 Hz,n=17,versus 4.7±0.7 Hz,n=18,P=0.03)and the depolarization-induced spikes in STN neurons,as well as enhanced the STN responses to feeding.Optogenetic experiments revealed that stimulation and inhibition of STN neurons respectively reduced(by 11%±6%,n=6,P=0.02)and enhanced(by 36%±15%,n=7,P=0.03)food intake only in the dark phase.In conclusion,our results support the hypothesis that STN neurons are activated by feeding behavior,depending on energy homeostatic status and the palatability of food,and modulation of these neurons is sufficient to regulate food intake.
