Multiphoton absorption of femtosecond laser pulses focused through an objective with high numerical aperture(NA)can be used to image and manipulate cellular and intracellular objects.This review highlights recent adva...Multiphoton absorption of femtosecond laser pulses focused through an objective with high numerical aperture(NA)can be used to image and manipulate cellular and intracellular objects.This review highlights recent advances in intracellular manipulation,including nanosurgery and labeling in living cells with femtosecond lasers.展开更多
A special device with photocurrent amplification function is reported. The device with long base region structure consists of dual route photodetectors and their amplifier. Two photodetectors with a space of 50 μm ...A special device with photocurrent amplification function is reported. The device with long base region structure consists of dual route photodetectors and their amplifier. Two photodetectors with a space of 50 μm are precisely located in this device. The device with current sensitivity of S ≥15 A/lm,static state current transmission coefficient of h FE ≥5 000, single route dark current of I D≥1 μA, high frequency current transmission coefficient modulus of | h fe |≥1 at 400 MHz is obtained. At present, the device has been tried out in展开更多
Probes that can undergo photoconversion in situ within cells are advantageous tools for live cell imaging in terms of precise spatial and temporal control.We herein present a new concept to construct photoconvertible ...Probes that can undergo photoconversion in situ within cells are advantageous tools for live cell imaging in terms of precise spatial and temporal control.We herein present a new concept to construct photoconvertible probes based on intramolecular oxygen direct arylation within cells.Xanthones bearing aryls(XO-Ars)were designed and prepared.XO-Ars undergo oxygen direct arylation under visible light irradiation in cells to afford xanthene derivatives(XE-Ars).XO-Ars initially accumulate in the endoplasmic reticulum(ER)with green emission and then migrate to the mitochondria with bright red emission.Sequential single-cell lighting up experiments show high spatiotemporal control ability and utility in single or multi-cell tracking.In addition,the photoproducts irradiated with optimized wavelength light source show excellent photodynamic therapy effects.As the duration of light exposure increases,the cells begin to undergo apoptosis.These innovative photoconvertible probes capable of migrating from ER to mitochondria driven by light provide a feasible approach for the in-situ monitoring of subcellular physiological events and cell apoptosis.展开更多
文摘Multiphoton absorption of femtosecond laser pulses focused through an objective with high numerical aperture(NA)can be used to image and manipulate cellular and intracellular objects.This review highlights recent advances in intracellular manipulation,including nanosurgery and labeling in living cells with femtosecond lasers.
文摘A special device with photocurrent amplification function is reported. The device with long base region structure consists of dual route photodetectors and their amplifier. Two photodetectors with a space of 50 μm are precisely located in this device. The device with current sensitivity of S ≥15 A/lm,static state current transmission coefficient of h FE ≥5 000, single route dark current of I D≥1 μA, high frequency current transmission coefficient modulus of | h fe |≥1 at 400 MHz is obtained. At present, the device has been tried out in
基金financially supported by National Natural Science Foundation of China(No.U21A20308)Foundation from Science and Technology Major Project of Xizang Autonomous Region of China(No.XZ202201ZD0001G)。
文摘Probes that can undergo photoconversion in situ within cells are advantageous tools for live cell imaging in terms of precise spatial and temporal control.We herein present a new concept to construct photoconvertible probes based on intramolecular oxygen direct arylation within cells.Xanthones bearing aryls(XO-Ars)were designed and prepared.XO-Ars undergo oxygen direct arylation under visible light irradiation in cells to afford xanthene derivatives(XE-Ars).XO-Ars initially accumulate in the endoplasmic reticulum(ER)with green emission and then migrate to the mitochondria with bright red emission.Sequential single-cell lighting up experiments show high spatiotemporal control ability and utility in single or multi-cell tracking.In addition,the photoproducts irradiated with optimized wavelength light source show excellent photodynamic therapy effects.As the duration of light exposure increases,the cells begin to undergo apoptosis.These innovative photoconvertible probes capable of migrating from ER to mitochondria driven by light provide a feasible approach for the in-situ monitoring of subcellular physiological events and cell apoptosis.
